Direct emissions are produced from residential and commercial activities in a variety of ways: 1  Combustion of natural gas and petroleum products for heating and cooking needs emits carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O).
Of red blood cell number. Classically this was the packed cell volume. blood was placed in a microtube and spun in a centrifuge. The rbcs settle to the bottom and one measures the height in the tube and reported as a percentage. Now determined by an automated machine. A typical hct is about 3 x the hemoglobin (hgb).
conversion into cell number is convenient (see below). Fig. 1: Growth curve of CHO cells in bioreactors obtained by mini PCV tubes from TPP. Samples were centrifuged during 1 minute at 2500 g in a swing bucket rotor before reading the packed cell volume. The maximum cell density as measured by the trypan blue method was about 8 million cells per ml. Are dilutions needed? No dilutions are necessary! If the packed cell volume exceeds the capacity of the capillary (e.g. for a 1 ml sample this is the case if the Vo PCV is higher than 0,5Vo),