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ï¼ 10 % salivary amylase solution. Page ï§ 11. Estimation of salivary amylase activity. ï¼A mixture of 5.0 mL 1% starch, 2 mL 1% NaCl solution and 2 mL phosphate buffer put in a test tube and then placed in a water bath ï¼At 38oC, 1 mL salivary enzyme solution added to the solution. An enzyme that breaks down starch into oligosaccharides through hydrolysis ï§ Secreted by the humanâs parotid glands and the pancreas ï§ Î±-Amylase ï§ Î²-Amylase ï§ Î³-Amylase. Page ï§ 8. Factors that may affect catalysis rates. ï§ Temperature ï§ pH ï§ Enzyme concentration ï§ Amount of substrate.