Absolute location describes the position of a place in a way that never changes, no matter your location. The location is identified by specific coordinates. The most common coordinate system is longitude and latitude, which describes a specific place on the earth's surface.
Nucleic Acids. Nucleotides join together through phosphodiester linkages between the 5' and 3' carbon atoms to form nucleic acids. The 3' -OH of the sugar group forms a bond with one of the negatively charged oxygens of the phosphate group attached to the 5' carbon of another sugar.
The negative charge on the phosphate group is stabilized as the charge is distributed all over the molecule. As DNA is negatively charged, it gets attracted towards positive electrode or anode in electrophoresis. The oxygen atoms in the phosphate group are negatively charged. In solution, DNA forms a phosphoric ester where a H atom of phosphoric acid is replaced by a nitrogen base and sugar molecule.