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The choice of the second buffer depends on your application: if you measure values below pH 7, use pH 4 as a second buffer. If you measure values above pH 7, use pH 10 as your second buffer. Some of our pH meter use 3 points calibration, keep in mind to always start with pH 7 first and others after.
Rinse and place the clean electrode in a pH 4 buffer. Allow 30 seconds to. stabilize, adjust the meter to read 4.0 and record the mV reading. The 4.0 pH mV reading should be 159 mV higher than value in pH 7 mV reading. ex. Example: pH 7 buffer is 10 mV; pH 4 buffer should read 169 mV or more.