6.Spin 5 minutes in microfuge. Function: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting.Function: sets an emulsion; biphasic solution.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Related Terms: Initial Public Offering; Leveraged Buyouts. Mezzanine financing is a hybrid between debt and equity. In a multi-tiered financing of an operation, for instances, the sources of money will be senior debt, senior subordinated debt, subordinated debt, mezzanine debt, and finally the owner's own equity.
In finance, mezzanine capital is any subordinated debt or preferred equity instrument that represents a claim on a company's assets which is senior only to that of the common shares.Mezzanine financings can be structured either as debt (typically an unsecured and subordinated note) or preferred stock.Mezzanine capital is often a more expensive financing source for a company than secured debt or senior debt.ezzanine financings can be structured either as debt (typically an unsecured and subordinated note) or preferred stock. Mezzanine capital is often a more expensive financing source for a company than secured debt or senior debt.