6.Spin 5 minutes in microfuge. Function: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting.Function: sets an emulsion; biphasic solution.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Related Terms: Initial Public Offering; Leveraged Buyouts. Mezzanine financing is a hybrid between debt and equity. In a multi-tiered financing of an operation, for instances, the sources of money will be senior debt, senior subordinated debt, subordinated debt, mezzanine debt, and finally the owner's own equity.
Salt Lending, the Ethereum based platform that aims to provide crypto asset backed loans to the public, has completed its ICO. Its native SALT tokens are now available on the market and on several exchanges, including the popular ShapeShift. Also read: Celsius Lending: The Battle Against Big Banks Has Begun