INSTANTER defined on the Free Online Law Dictionary. law terminology definitions including drugs, abbreviations, equipment, devices, and more from almost all law specialties. law-dictionary.org Law Dictionary
A northern blot is a laboratory method used to detect specific RNA molecules among a mixture of RNA. Northern blotting can be used to analyze a sample of RNA from a particular tissue or cell type in order to measure the RNA expression of particular genes.
Northern blotting is used for detecting RNA fragments, instead of DNA fragments. The technique is called Northern simply because it is similar to Southern, not because it was invented by a person named Northern.In the Southern blotting, DNA fragments are denatured with alkaline solution.b) The DNA fragments in the gel are denatured with alkaline solution and transferred onto a nitrocellulose filter or nylon membrane by blotting, preserving the distribution of the DNA fragments in the gel. (c) The nitrocellulose filter is incubated with a specific probe.