6.Spin 5 minutes in microfuge. Function: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting.Function: sets an emulsion; biphasic solution.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Related Terms: Initial Public Offering; Leveraged Buyouts. Mezzanine financing is a hybrid between debt and equity. In a multi-tiered financing of an operation, for instances, the sources of money will be senior debt, senior subordinated debt, subordinated debt, mezzanine debt, and finally the owner's own equity.
Mezzanine financing is a sometimes confusing part of the capital structure in a real estate transaction. Part of the reason for this is that the term mezzanine is really a catch-all for an entire category of non-senior mortgage debt, non-common equity instruments that can fill a capitalization gap between them. Mezzanine (âmiddleâ) financing can take the form of debt or equity, more specifically: