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Answers. Best Answer: That is precisely what it requires as also denoted by the word oxidative phosphorylation. It happens through the electron transport chain process and the proton gradient it creates. Substrate level phosphorylation does not require oxygen, it occurs during glycolysis and the Krebs cycle.Source(s): greendawn Â· 7 years ago.t happens through the electron transport chain process and the proton gradient it creates. Substrate level phosphorylation does not require oxygen, it occurs during glycolysis and the Krebs cycle. Source(s): greendawn Â· 7 years ago.
Oxidative phosphorylation occurs in the mitochondria, and the two reactions (oxidation of NADH or FADH 2 and phosphorylation to generate ATP) are coupled by a proton gradient across the inner membrane of the mitochondria (Figure 9).he oxidation reaction for NADH has a larger, but negative, D G than the positive D G required for the formation of ATP from ADP and phosphate. This set of coupled reactions is so important that it has been given a special name: oxidative phosphorylation.