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Column chromatography is one of the most common methods of protein purification. Like many of the techniques on this site, it is as much an art form as a science. Proteins vary hugely in their properties, and the different types of column chromatography allow you to exploit those differences.
PAPER CHROMATOGRAPHY This page is an introduction to paper chromatography-including two way chromatography. Carrying out paper chromatography Background Chromatography is used to separate mixtures of substances into their components.All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).he mobile phase is a suitable liquid solvent or mixture of solvents. Producing a paper chromatogram You probably used paper chromatography as one of the first things you ever did in chemistry to separate out mixtures of coloured dyes-for example, the dyes which make up a particular ink.