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DHEA supplementation can increase testosterone levels quite significantly in middle-aged and older men, and in menopausal women. However, these effects are â for some reason â highly unreliable in younger guys.
Although higher testosterone levels are seen with abstinence, orgasm does not acutely affect testosterone levels in the blood. Although there is not much evidence for the spike in testosterone during abstinence, there does not appear to be counter evidence at the moment; it is an understudied topic.