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Automated fraction collector and sampler for chromatographic techniques. Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms.
Background. Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).he mobile phase is a suitable liquid solvent or mixture of solvents. Producing a paper chromatogram You probably used paper chromatography as one of the first things you ever did in chemistry to separate out mixtures of coloured dyes-for example, the dyes which make up a particular ink.