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12.6 Include an unopened negative control plate for each sampling day. 13 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.3 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.
Sanders et al. (1978) found that theexperimental incubation period varied with the water temperature. At tempera-tures above 11Â°C. initial losses occurred 30 to 35 d after exposure. At tempera-tures between 7 and 11 C, the incubation period was 60 to 90 d or longer.SEASONAL Incidence Outbreaks of BKD occur in cultured trout populations more often in thespring than at any other time of the year. Reported cases more than double infrequency in March, peak in May, and nearly cease in July.ausing BKD is a small Gram-positive diplobacillus named Renibacterium salm-oninarum (Sanders and Fryer 1980). The pathogen can be transmitted from fishto fish (Mitchum and Sherman 1981) or from adults to their progeny via eggs(Bullock 1980; Bullock et al.