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Column chromatography is one of the most common methods of protein purification. Like many of the techniques on this site, it is as much an art form as a science. Proteins vary hugely in their properties, and the different types of column chromatography allow you to exploit those differences.
Chromatography (/ËkroÊmÉËtÉÉ¡rÉfi/ ; from Greek ÏÏá¿¶Î¼Î± chroma which means color and Î³ÏÎ¬ÏÎµÎ¹Î½ graphein to write) is the collective term for a set of laboratory techniques for the separation of mixtures.he purpose of preparative chromatography is to separate the components of a mixture for more advanced use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture.