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12.6 Include an unopened negative control plate for each sampling day. 13 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.3 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.
Agar Plates. In agar plates, the agar (for solidification) and liquid growth medium of the desired bacteria for growth combine. Agar plates are then incubated at the desired temperature for growth.hen scientists want to grow bacteria, a number of factors affect the incubation technique used. Individual temperature requirements, food required for growth and pH requirements represent just a few of the things to consider before incubation.