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12.6 Include an unopened negative control plate for each sampling day. 13 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.3 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.
Make 6 identical plates and streak the same 8 bacteria on each plate then incubate each plate at a different temperature. Here are some suggested temperatures. 1  several days at 4 C. 2  2 days at 4 C; then 1 or 2 days at r.t.( 3 r.t. is room temperature). 4  two days at room temperture. 5  one or two days at 37 C.ake 6 identical plates and streak the same 8 bacteria on each plate then incubate each plate at a different temperature. Here are some suggested temperatures. 1  several days at 4 C. 2  2 days at 4 C; then 1 or 2 days at r.t.