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Column chromatography is one of the most common methods of protein purification. Like many of the techniques on this site, it is as much an art form as a science. Proteins vary hugely in their properties, and the different types of column chromatography allow you to exploit those differences.
Gas chromatography is suitable for analyzing organic compounds and uses a gaseous mobile phase and a liquid or solid stationary phase. High-performance liquid chromatography is a highly efficient method, as it uses high-pressure pumps to improve the separation process. Metal ions and organic compounds are separated by liquid chromatography, a method that uses a solvent as a mobile phase.