It is the amount a business charges above their direct cost. If your contractor has a 1.50 markup (which is reasonable for a remodeling contractor), that means that if the estimated cost for a job is $10,000, they'll multiply the $10,000 x 1.50 and arrive at a $15,000 sales price.ow many people who know little about business and even less about the costs of running a business will say, Oh, look at that crook. He is making $5,000 profit on my job.. Nope, not true.
Partition chromatography is often preferred for the latter, wherein an inert solid (often silica gel) is coated with a liquid phase. Hydrophobic interaction chromatography (HIC) is a special form of surface adsorption chromatography. The materials to be separated should be at least partially hydrophobic in nature.
These methods are based on gel filtration chromatography, also called molecular sieve chromatography, which is a form of size-exclusion chromatography. Desalting and buffer exchange are two of the most common gel filtration chromatography applications, and they can be performed using the same resin. Desalting and buffer exchange both entail recovering the components of a sample in whatever buffer is used to pre-equilibrate the small, porous polymer beads (resin). Desalting occurs when buffer salts and other small molecules are removed from a sample in exchange for water (with the resin being pre-equilibrated in water).