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Column chromatography is one of the most common methods of protein purification. Like many of the techniques on this site, it is as much an art form as a science. Proteins vary hugely in their properties, and the different types of column chromatography allow you to exploit those differences.
One difference between column and thin layer chromatography is that  in column chromatography the stationary phase is powdered adsorbent  placed in a vertical glass column. â¦In thin lay chromatography, the  stationary phase is also powdered adsorbent, but it is fixed to a  plate made from glass, aluminum, or plastic.