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Methyl red (2-(N,N-dimethyl-4-aminophenyl) azobenzenecarboxylic acid) , also called C.I. Acid Red 2, is an indicator dye that turns red in acidic solutions.It is an azo dye, and is a dark red crystalline powder. Methyl red is a pH indicator; it is red in pH under 4.4, yellow in pH over 6.2, and orange in between, with a pKa of 5.1.ethyl red (2-(N,N-dimethyl-4-aminophenyl) azobenzenecarboxylic acid) , also called C.I. Acid Red 2, is an indicator dye that turns red in acidic solutions.
When methyl red is added, if acidic end products are present, the methyl red will stay red. NOTE: Methyl red differs from Phenol red (which is used in the fermentation test and the MSA plates) in that it is yellow at pH 6.2 and above and red at pH 4.4 and below.Phenol red turns yellow below a pH of 6.8.ethyl Red (MR) and Voges-Proskauer (VP) broth is used as a part of the IMViC tests as the medium in which both the Methyl Red and Voges-Prosakuer tests can be performed. It is a simple broth that contains peptone, buffers, and dextrose or glucose.