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12.6 Include an unopened negative control plate for each sampling day. 13 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.3 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.
2 Keep plates at room temperature or incubate at 20-25 Â°C for 2-3 days. Fungi grow more successfully at lower temperatures. Do not incubate at human body temperature (or above 30 Â°C) â this reduces the risk of culturing microbes that are pathogens to humans.3 Reducing the temperature to 4 Â°C will slow the growth of any cultures â so you can show your students a 2-3 day growth if your lessons are a week apart.4 All inoculated plates must be taped before incubation to ensure they cannot be opened accidentally.r you can stop the growth of a culture completely by placing a piece of filter paper into the lid of the inverted plate. Add a little 40% methanal solution carefully to soak the filter paper and replace the base. Leave for 24 hours. Remove the filter paper, remove any surplus liquid, and reseal the plate.