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From Wikipedia, the free encyclopedia. A true breeding organism, sometimes also called a purebred, is an organism that always passes down certain phenotypic traits (i.e. physically expressed traits) to its offspring.n organism is referred to as true breeding for each trait to which this applies, and the term true breeding is also used to describe individual genetic traits.
A pure culture is one in which only one kind of microbial species is found whereas in mixed culture two or more microbial species formed colonies. pure culture is a culture consisting of only one strain. YOU CAN OBTAIN A PURE CULTURE BY PICKING OUT A SMALL PORTION OF THE MIXED CULTURE(which will form the pure culture) AND GROWING THEM ON A NEW CULTURE MEDIA.