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12.6 Include an unopened negative control plate for each sampling day. 13 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.3 INCUBATION OF PLATES 13.1 Incubate inverted TSA and SDA Rodac plates for 5-7 days at 20-25Â°C. 13.2 After the incubation period above, move the TSA Rodac plates for incubation for at least 2-3 days at 30-35Â°C.
From this step on there are two commonly used detection systems, immunofluorescence or immunoperoxidase. For the immunofluorescence method, slides are next incubated with 10% normal goat serum for 37oC for 45 min to block nonspecific binding. This is followed by incubation with an appropriate dilution of adduct specific antibody in blocking solution for 45 min at 37oC or at 4oC overnight.