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Automated fraction collector and sampler for chromatographic techniques. Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms.
In flash column chromatography the most commonly used stationary phases are silica gel (SiO2) and alumina (Al2O3). Other stationary phases are available for specific uses (Table 1). Figure 2 shows the general structure of normal phase, reversed phase and specialized silica.