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Column chromatography is one of the most common methods of protein purification. Like many of the techniques on this site, it is as much an art form as a science. Proteins vary hugely in their properties, and the different types of column chromatography allow you to exploit those differences.
Paper chromatography. This form of chromatography is one of the first methods of separating compounds. Both paper and thin layer chromatography are examples of planar chromatography. However, compared with thin layer chromatography that works in similar way, paper chromatography is less sensitive and slower.