After proteins are digested on the Enzymate BEH Pepsin Column, the valve is switched and the peptic peptides are separated using reversed-phase UPLC. The trap column used is a VanGuard 2.1 x 5 mm Pre-Column containing 1.7 Î¼m ACQUITY UPLC BEH C 18 packing material (part number 186003975).
Sweet Caroline by Neil Diamond. Diamond wrote this song about his second wife, Marcia Murphey, who he married in 1969 (they divorced in 1995). He needed a three-syllable name to fit the melody, however, so Sweet Marcia didn't work.
Dear Jessie is a song by American singer Madonna from her fourth studio album Like a Prayer (1989). It was released as the fifth single from the album on December 10, 1989 by Sire Records. Written and produced by Madonna and Patrick Leonard, the song was inspired by Leonard's daughter Jessie. The release of Dear Jessie was limited to the United Kingdom, certain other European countries, Australia and Japan.