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The notion that sex could be determined by chromosomes was a new idea. Prior to Henking, McClung, and Sutton's reports, sex determination was attributed to factors other than gametes, such as the environment in which egg cells existed.
It is the protein that determines the trait. This is called central dogma of life. Fig 1.a.2 Central dogma of life. Notes: Genes are DNA sequences instruct cells to produce particular proteins, which in turn determine traits. Chromosomes are strings of genes. Mutations are changes in gene's DNA sequence.