After proteins are digested on the Enzymate BEH Pepsin Column, the valve is switched and the peptic peptides are separated using reversed-phase UPLC. The trap column used is a VanGuard 2.1 x 5 mm Pre-Column containing 1.7 Î¼m ACQUITY UPLC BEH C 18 packing material (part number 186003975).
Sweet Caroline by Neil Diamond. Diamond wrote this song about his second wife, Marcia Murphey, who he married in 1969 (they divorced in 1995). He needed a three-syllable name to fit the melody, however, so Sweet Marcia didn't work.
Caroline Bingley is Bingley's youngest sister. She is a superficial and selfish girl, possessing all of Darcy's class prejudice but none of his honor and virtue. Throughout the novel, she panders to Darcy in an attempt to win his affections, but to no avail. Her cruelty towards Jane and Elizabeth marks her as a generally unpleasant character.