After proteins are digested on the Enzymate BEH Pepsin Column, the valve is switched and the peptic peptides are separated using reversed-phase UPLC. The trap column used is a VanGuard 2.1 x 5 mm Pre-Column containing 1.7 Î¼m ACQUITY UPLC BEH C 18 packing material (part number 186003975).
Sweet Caroline by Neil Diamond. Diamond wrote this song about his second wife, Marcia Murphey, who he married in 1969 (they divorced in 1995). He needed a three-syllable name to fit the melody, however, so Sweet Marcia didn't work.
Dear Jessie by Madonna. Madonna wrote this with Patrick Leonard, who collaborated with her on several of her '80s hits. The song is a pop lullaby which displays Madonna's maternal side. It was written for Patrick Leonard's daughter, Jessie.