After proteins are digested on the Enzymate BEH Pepsin Column, the valve is switched and the peptic peptides are separated using reversed-phase UPLC. The trap column used is a VanGuard 2.1 x 5 mm Pre-Column containing 1.7 Î¼m ACQUITY UPLC BEH C 18 packing material (part number 186003975).
Sweet Caroline by Neil Diamond. Diamond wrote this song about his second wife, Marcia Murphey, who he married in 1969 (they divorced in 1995). He needed a three-syllable name to fit the melody, however, so Sweet Marcia didn't work.
In the original book series, Caroline's parents were Henry and Charlotte Quiner. She was the fifth of eight children. She had two sisters, Eliza and Martha, and three brothers, Joseph, Thomas (known as Uncle Tom in These Happy Golden Years) and Henry (known as Uncle Henry in Little House in the Big Woods and On the Shores of Silver Lake). She had a stepsister, Lottie, and a sister, Martha, who died before Caroline was born.