1 Rhythmic periods of pulling and resting are usually applied, with each position being held for up to 10 seconds.  Mechanical Cervical Traction. Mechanical traction for your neck involves using a harness that attaches to your head and neck while you're lying down on your back.
Sandwich ELISA Protocol. An Enzyme-linked immunosorbent assay or Sandwich ELISA is a scientific technique used by researchers to detect the presence of an antibody or an antigen in a sample.An ELISA can also be used as a diagnostic tool in medicine.andwich ELISA Protocol. An Enzyme-linked immunosorbent assay or Sandwich ELISA is a scientific technique used by researchers to detect the presence of an antibody or an antigen in a sample.
ELISA is a popular format of wet-lab type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. The secondary antibody, specific to the primary antibody, is added. 2  This second antibody is coupled to the enzyme. 3  A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. 4  The reaction is stopped to prevent eventual saturation of the signal.