After proteins are digested on the Enzymate BEH Pepsin Column, the valve is switched and the peptic peptides are separated using reversed-phase UPLC. The trap column used is a VanGuard 2.1 x 5 mm Pre-Column containing 1.7 Î¼m ACQUITY UPLC BEH C 18 packing material (part number 186003975).
Sweet Caroline by Neil Diamond. Diamond wrote this song about his second wife, Marcia Murphey, who he married in 1969 (they divorced in 1995). He needed a three-syllable name to fit the melody, however, so Sweet Marcia didn't work.
Listen to âLast Kissâ by J Frank Wilson and the Cavaliers. âLast Kissâ was written about three teenagers who were killed in a car crash in 1956. The original version by Wayne Cochran didnât make the charts and was soon forgotten.ast Kiss-Pearl Jam. The history of âLast Kissâ is stranger than fiction. Written in 1960, the tragic song has hit the Billboard Top 40 twice, turning two bands into one-hit wonders.