After proteins are digested on the Enzymate BEH Pepsin Column, the valve is switched and the peptic peptides are separated using reversed-phase UPLC. The trap column used is a VanGuard 2.1 x 5 mm Pre-Column containing 1.7 Î¼m ACQUITY UPLC BEH C 18 packing material (part number 186003975).
Sweet Caroline by Neil Diamond. Diamond wrote this song about his second wife, Marcia Murphey, who he married in 1969 (they divorced in 1995). He needed a three-syllable name to fit the melody, however, so Sweet Marcia didn't work.
In the autumn of 1969, Diamond performed Sweet Caroline on several television shows. It later reached No. 8 on the UK singles chart in 1971. In a 2007 interview, Diamond stated the inspiration for his song was John F. Kennedy's daughter, Caroline, who was eleven years old at the time it was released.