There are very few of Ca dependent proteases. I am not sure kinases need metals as cofactors (activation is a different thing; that's signaling). Alkaline phosphatase needs Zn. But I guess this is not as common as the dependence of nucleases on metal ions. In any case we do use a protein inhibitor cocktail while doing extraction.
Summary. 1  Incorporation and corporation cannot be differentiated as one leads to the other. 2  While incorporation is the process, corporation is the organisation formed out of this process.
An incorporated business, or a corporation, is a separate entity from the business owner and has natural rights.Conversely, a business owner and an unincorporated business are the same, and the owner personally bears all results of the business.Unincorporated businesses are usually sole proprietor or partnership companies.The main difference between an incorporated and unincorporated business is the way owners shoulder business activities.n incorporated business, or a corporation, is a separate entity from the business owner and has natural rights.