The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Kirkenes is located in the extreme north eastern part of Norway on the Boekfjorden, bÃ¸kfjorden a branch of The Varanger, fjord which is a vast bay connected to The Barents sea near The-Russian norwegian. Border the town is situated about 240 (miles 400) km north of The Arctic. Circle: Photo Monica / Horn Hurtigruten. archive