The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Norway is situated in the western part of the Scandinavian peninsula. It extends about 1,100 mi (1,770 km) from the North Sea along the Norwegian Sea to more than 300 mi (483 km) above the Arctic Circle, the farthest north of any European country. It is slightly larger than New Mexico.