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Classification of culture media used in Microbiology laboratory on the basis of consistency. 1  Solid medium. solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. 2  Semisolid media. They are prepared with agar at concentrations of 0.5% or less. 3  Liquid (Broth) medium.
Microbiology Theory: Media Preparation. Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for their needs, in the laboratory, however, these requirements must be met by a culture medium.