The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Norway. Norway is located in Northern Europe on the Scandinavian Peninsula between the North Sea and the North Atlantic Ocean. It has an area of 125,020 square miles (323,802 sq km) and 15,626 miles (25,148 km) of coastline.inland is a Northern European country that is located between Sweden and Russia and northern border with Norway. It covers a total land area of 130,558 square miles (338,145 sq km) and has 776 miles (1,250 km) of coastline.