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In the process, the nucleus of an egg is inserted into the cytoplasm of an egg from a donor female which has had its nucleus removed, but still contains the donor female's mtDNA. The composite egg is then fertilized with the male's sperm.
They produce sperm cells that contain either an X chromosome or no sex chromosome, which is designated as O. The females are XX and produce egg cells that contain an X chromosome. If an X sperm cell fertilizes an egg, the resulting zygote will be XX or female. If a sperm cell containing no sex chromosome fertilizes an egg, the resulting zygote will be XO or male. Sex Chromosomes Z-W