The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
The Hovgaard Ridge is a plateau in the deep Arctic Sea, located a good 400 kilometres off of Greenland's eastern coast. Found at a depth of 1,200 metres the five lineaments are the deepest iceberg scours found to date in the Arctic. The scours are as much as four kilometres long and 15 metres in depth. Such scours are a window into the past.