The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
The Oslofjord (Norwegian: Oslofjorden, English: Oslo Fjord) is an inlet in the south-east of Norway, stretching from an imaginary line between the Torbjoernskjaer torbjÃ¸rnskjÃ¦r And faerder fÃ¦rder lighthouses and Down to langesund in the South to oslo in. the northhe Oslofjord has Norwayâs highest all year temperature: 7.5 degrees Celsius. February is the coldest month in the fjord with -1.3 degrees Celsius, while July normally has 17.2 degrees Celsius. The islands in the middle of the fjord are among Norwayâs warmest with high summer temperatures and moderate winters.