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Classification of culture media used in Microbiology laboratory on the basis of consistency. 1  Solid medium. solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. 2  Semisolid media. They are prepared with agar at concentrations of 0.5% or less. 3  Liquid (Broth) medium.
This list of simple media is all you need to maintain a culture collection. At the peak, in 1985, my collection of microbial cultures had 2000 strains of 250 species. These are the only media I used for stock cultures. ** marks the media I use for many species, * marks media I use for a few species.I needed the other media only for rare species. Notice the abbreviation listed before each medium. I mark that abbreviation on each tube of the medium and use it on all my web pages.A Nutrient Agar. Supports the growth of many common bacteria from the intestines and many other species. Nutrient agar is the most widely used medium in highschools, but not in my laboratory. TGY supports the growth of many more species.