The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Homer Spit, Homer, Alaska The Homer Spit is a geographical landmark located in Homer, Alaska on the southern tip of the Kenai Peninsula. The spit is a 4.5-mile (7.2 km) long piece of land jutting out into Kachemak Bay. The spit is also home to the Homer Boat Harbor.