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In the process, the nucleus of an egg is inserted into the cytoplasm of an egg from a donor female which has had its nucleus removed, but still contains the donor female's mtDNA. The composite egg is then fertilized with the male's sperm.
In mammals, the sex of the offspring is determined by the sperm cell: a spermatozoon bearing a X chromosome will lead to a female (XX) offspring, while one bearing a Y chromosome will lead to a male (XY) offspring. Sperm cells were first observed by Anton van Leeuwenhoek in 1677.