The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Report Abuse. The northernmost point on land is Kaffeklubben Island, north of Greenland (83Â°40â²N, 29Â°50â²W). At 70Â° 39' 48, Hammerfest is the northernmost city in the world The southernmost point of the World and the southernmost point on land is the geographic South Pole, which is on the continent of Antarctica.t 70Â° 39' 48, Hammerfest is the northernmost city in the world The southernmost point of the World and the southernmost point on land is the geographic South Pole, which is on the continent of Antarctica.