There are very few of Ca dependent proteases. I am not sure kinases need metals as cofactors (activation is a different thing; that's signaling). Alkaline phosphatase needs Zn. But I guess this is not as common as the dependence of nucleases on metal ions. In any case we do use a protein inhibitor cocktail while doing extraction.
Summary. 1  Incorporation and corporation cannot be differentiated as one leads to the other. 2  While incorporation is the process, corporation is the organisation formed out of this process.
How is a corporation different from a sole proprietorship? Corporations can sell stock to raise money for the business. Steven and his brothers want to start a plumbing business together.