The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Kotzebue, Alaska sits on a 3-mile-long spit at the northwest end of Baldwin Peninsula in Kotzebue Sound and about 30 miles above the Arctic Circle. It may look isolated, but this town is the hub of commerce, culture and outdoor activity for the northwestern Arctic region. Kotzebue is a popular stop for visitors on airline package tours who want to see a bit of the Arctic landscape and visit the Innaigvik Education and Information Center to learn about the people. The northwest Arctic is a haven for wildlife and a heaven for who subsist on it or merely want to observe it.