The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
We stop at Stykkisholmur town, built around a natural harbour by Danish and Norwegian merchants. This charming fishing town is the largest on the Snaefellsnes Peninsula, located on a promontory overlooking the beautiful Breidarfjordur bay. The majestic Snaefellsjokull gives you breathtaking views over the peninsula and people who believe in esoteric matters say this glacier is surrounded by a unique energy field.