DNA Polymerase III of Escherichia coli PURIFICATION AND IDENTIFICATION OF SUBUNITS* (Received for publication, July IO, 1978) DNA polymerase III, the core of the DNA polymerase III holoenzyme, has been purified Z&000-fold to 97% homogeneity from Escherichiu coli HMS-83.he denaturing second dimension. DNA Polymerase III of E. coli 1751 1st dimension polymerase III 7 r impurity I B - --a ---\--, = , impurities / ,130,000 - 94,000 - 30,000 I IL 0.93 0.63 0.17 Rf I FIG. 3. A, two-dimensional electrophoresis of DNA polymerase III.
lift, raise, rear, elevate, hoist, heave, boost mean to move from a lower to a higher place or position. lift usually implies exerting effort to overcome resistance of weight. lift the chair while I vacuum; raise carries a stronger implication of bringing up to the vertical or to a high position. scouts raising a flagpole
Climb(verb) to ascend as if with effort; to rise to a higher point. Climb(verb) to ascend or creep upward by twining about a support, or by attaching itself by tendrils, rootlets, etc., to a support or upright surface. Climb(verb) to ascend, as by means of the hands and feet, or laboriously or slowly; to mount.