There are very few of Ca dependent proteases. I am not sure kinases need metals as cofactors (activation is a different thing; that's signaling). Alkaline phosphatase needs Zn. But I guess this is not as common as the dependence of nucleases on metal ions. In any case we do use a protein inhibitor cocktail while doing extraction.
Summary. 1  Incorporation and corporation cannot be differentiated as one leads to the other. 2  While incorporation is the process, corporation is the organisation formed out of this process.
LLC (Limited Liability Company) and an S corporation are both corporate structures that, in the United States, allow pass-through taxation. The main differences between an S corp. and LLC are: 1  S corporations are more restrictive on who the shareholders (owners) of the company can be.2  S corporations are required to pay a salary to those owners who work for the company and own more than 2% of the company. LLC members are the owners of the LLC much as shareholders are the owners of a corporation or the partners of a partnership. 2  Like shareholders, a member's liability to repay the LLC's obligations is limited to his or her capital contribution. 3  Members may be natural persons, corporations, partnerships, or other LLCs.