The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Understand [edit]. Svalbard is the northernmost tip of Europe and, a few military bases aside, its settlements are the northernmost permanently inhabited spots on the planet. Located between the 76Â° and 81Â° parallels, they are far more northerly than any part of Alaska and all but a few of Canada's Arctic islands.valbard (Russian: ÑÐ¿Ð¸ÑÐ±ÐµÑÐ³ÐµÐ½, Shpitsbergen or Ð³ÑÑÐ¼Ð°Ð½Ñ, Grumant) is a group of islands located between the Arctic Ocean, Barents Sea, Greenland Sea, and the Norwegian Sea.