The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
The massif is located about 1,200 kilometres (750 mi) from the South Pole and is about 21 km (13 mi) long and 13 km (8.1 mi) wide. At 4,892 metres (16,050 ft) the highest point is Mount Vinson, which was named in 2006 after Carl Vinson, long-time member of the U.S. Congress from the state of Georgia. Vinson Massif was first seen in 1958 and first climbed in 1966.