The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Bouvet Island is an uninhabited subantarctic high island and dependency of Norway located in the South Atlantic Ocean at 54Â°25.8â²S 3Â°22.8â²Eï»¿ / ï»¿54.4300Â°S 3.3800Â°Eï»¿ / -54.4300; 3.3800Coordinates: 54Â°25.8â²S 3Â°22.8â²Eï»¿ / ï»¿54.4300Â°S 3.3800Â°Eï»¿ / -54.4300; 3.3800, thus putting it north of and outside the Antarctic Treaty System. It lies at the southern end of the Mid-Atlantic Ridge and is the most remote island in the world, approximately 2,600 kilometres south-southwest ...