The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
The archipelago is the northernmost part of Norway. Three islands are populated: Spitsbergen, Bear Island and Hopen. The largest settlement is Longyearbyen. The Svalbard Treaty recognises Norwegian sovereignty over Svalbard and the 1925 Svalbard Act makes Svalbard a full part of the Norwegian Kingdom.valbard consists of a group of islands in the Arctic Ocean ranging from 76Â° to 81Â° North and 10Â° to 35Â° East, and forms the northern-most part of Norway and the northern-most lands of Europe.