The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Europe Nordic countries Norway. Svalbard (Russian: ÑÐ¿Ð¸ÑÐ±ÐµÑÐ³ÐµÐ½, Shpitsbergen or Ð³ÑÑÐ¼Ð°Ð½Ñ, Grumant) is a group of islands located between the Arctic Ocean, Barents Sea, Greenland Sea, and the Norwegian Sea. The area is sometimes referred to as Spitsbergen, the main island with all the settlements.ll settlements in Svalbard are located on the main island of Spitsbergen (or Vest-Spitsbergen). 1  Barentsburg â sole remaining Russian settlement, population 400. 2  Hornsund â Polish research station, population 10 in winter, around 20â30 in summer.