The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Discover our favourite destinations in Norway. 1  Spitsbergen. 2  Lyngenfjord and Alps. 3  SommarÃ¸y. 4  Alta. 5  Kirkenes. 6  Senja. 7  TromsÃ¸. 8  Oslo.