The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Bouvet Island (Norwegian: BouvetÃ¸ya ) is an uninhabited subantarctic high island and dependency of Norway located in the South Atlantic Ocean at 54Â°25.8â²S 3Â°22.8â²E / 54.4300Â°S 3.3800Â°E / -54.4300; 3.3800Coordinates: 54Â°25.8â²S 3Â°22.8â²E / 54.4300Â°S 3.3800Â°E / -54.4300; 3.3800, thus putting it north of and outside the Antarctic Treaty System.