The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Blup Blup Island is a small forested island of the northern coast of Papua New Guinea about 30 km offshore from Cape Girgir and is considered part of the Schouten Islands. It is located at Latitude S 3Â°30'46 and Longitude E 144Â°35'16.