The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
qaanaaq Qaanaaq is located in the northwest of the country in the Baffin Bay on the Hayes Peninsula at Inglefield Fjord , about 200 kilometers southwest of the geomagnetic North Pole . Near the village, about 90 miles as the crow flies to the south, is the Thule Air Base .