The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Svalbard is an archipelago positioned in the Arctic Ocean to the north of mainland Europe, about midway between Norway and the North Pole. The archipelago is the northernmost part of the Kingdom of Norway. Total population is just over 2,200, with most people living in Longyearbyen on the rugged island of Spitsbergen.