The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
RELATIVE LOCATION: Located entirely on the Scandinavian Peninsula in far northern Europe, Norway is geographically positioned in both the northern and eastern hemispheres. Norway is bordered by the countries of Finland, Sweden and Russia, and by the Atlantic Ocean, Arctic Ocean, as well as the Barents Sea, North Sea and Norwegian Sea.