The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
Longyearbyen (Norwegian pronunciation: (literally The Longyear Town) is the largest settlement and the administrative centre of Svalbard, Norway. As of December 2015, the town had a population of 2,144. Longyearbyen is located in the Longyear Valley and on the shore of Adventfjorden, a bay of Isfjorden located on the west coast of Spitsbergen. Since 2002, Longyearbyen Community Council has had many of the same responsibilities of a municipality, including utilities, education, cultural facilitie