The proportion of Tris and glycine in the transfer buffer is not necessarily the same as for wet transfer; consult the apparatus manufacturer's protocol. A standard recipe is 48 mM Tris, 39 mM glycine, 0.04% SDS, 20% methanol. Two types of membrane are available: nitrocellulose and PVDF (positively charged nylon).
Spitsbergen (formerly known as West Spitsbergen; Norwegian: Vest Spitsbergen or Vestspitsbergen) is the largest and only permanently populated island of the Svalbard archipelago in northern Norway.
The Galapagos Archipelago is a cluster of some 13 volcanic islands and associated islets and rocks located just under the equator, about 600 miles (1000km) west of Ecuador in South America. The oldest of the islands are about 4 million years old and the youngest are still in the process of being formed.