splicing, is currently the most commonly used fusion splicing technology. It is more expensive for its complex technology, more powerful and flexible, and less sensitive to variations in the cable and environment.
DNA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA. It accomplishes this by creating phosphodiester bonds between the 3' end of one nucleotide and the 5' end of the next nucleotide.
In the final step of DNA ligation, unadenylyl-. ated DNA ligase is required for the generation of a phosphodiester bond. and catalyzes displacement of the AMP residue through attack by the ad-. jacent 3 -hydroxyl group on the adenylylated site (Fig. 1). The active site of mammalian DNA ligase I has been identified by.