Operating on spiritual side of your individuality can bring you to the great heights, and drop you off if you neglect your spiritual identity. You are always looking for an opportunity to investigate the unknown, to use and show your mental abilities, to find the purpose and meaning of life. You want to grow wise and to understand people and things.
Typically, PCR consists of usually 20-40 repeated temperature changes, called cycles, where each cycles usually contains three steps. It is necessary to perform many cycles because that way the end results will consist mainly of the desired DNA.
The number of cycles depends on the amount of DNA available and how much PCR product you want to yield. To carry out the PCR we will use a thermal cycler that is programmed with a protocol that goes through all three steps of a cycle, for a total of 35 cycles. See PCR animation. Other reagents necessary for PCR.