Operating on spiritual side of your individuality can bring you to the great heights, and drop you off if you neglect your spiritual identity. You are always looking for an opportunity to investigate the unknown, to use and show your mental abilities, to find the purpose and meaning of life. You want to grow wise and to understand people and things.
Typically, PCR consists of usually 20-40 repeated temperature changes, called cycles, where each cycles usually contains three steps. It is necessary to perform many cycles because that way the end results will consist mainly of the desired DNA.
Commercial real-time PCR instruments typically monitor fluorescence once each cycle for quantification and at the end of PCR for melting analysis. Measurements for quantification are taken at the same temperature each cycle to avoid any effect of temperature on fluorescence.