Operating on spiritual side of your individuality can bring you to the great heights, and drop you off if you neglect your spiritual identity. You are always looking for an opportunity to investigate the unknown, to use and show your mental abilities, to find the purpose and meaning of life. You want to grow wise and to understand people and things.
Typically, PCR consists of usually 20-40 repeated temperature changes, called cycles, where each cycles usually contains three steps. It is necessary to perform many cycles because that way the end results will consist mainly of the desired DNA.
A third reason your temperature gauge reads high could be because the thermostat is broken. If this is the case, you may need a coolant temperature switch replacement. The last reason the temperature gauge could read high is because of a water pump, or water pump gasket failure.