splicing, is currently the most commonly used fusion splicing technology. It is more expensive for its complex technology, more powerful and flexible, and less sensitive to variations in the cable and environment.
DNA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA. It accomplishes this by creating phosphodiester bonds between the 3' end of one nucleotide and the 5' end of the next nucleotide.
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule.hen an incorrect base pair is recognized, DNA polymerase moves backwards by one base pair of DNA. The 3'-5' exonuclease activity of the enzyme allows the incorrect base pair to be excised (this activity is known as proofreading).