What would cause lack of power and rattling during acceleration? It depends on if you have a carburetor or fuel injection but it is probably in the throttle body. You could have trash in your throttle body or the throttle body is worn out.
In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.1.1) that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. DNA ligase III: complexes with DNA repair protein XRCC1 to aid in sealing DNA during the process of nucleotide excision repair and recombinant fragments. 2  Of the all known mammalian DNA ligases, only Lig III has been found to be present in mitochondria. 3  DNA ligase IV: complexes with XRCC4.
Cutting and Splicing DNA Molecules. If you want to insert a piece of new DNA (foreign DNA) into an existing strand you'd have to cut the existing strand, somehow position the foreign DNA, and then join the strands. This joining is called ligation and can be done with the enzyme DNA ligase. Restriction enzymes are produced by bacteria and function to protect bacteria from foreign DNA (like viruses). Restriction enzymes work by degrading foreign DNA. Restriction enzymes recognize and cut the sugar phosphate backbone of the DNA molecule at specific sites on a strand of DNA. The restriction enzyme recognition site is determined by a specific sequence of nucleotides. Thousands of restriction enzymes have been isolated, although many share the same recognition sequence.