splicing, is currently the most commonly used fusion splicing technology. It is more expensive for its complex technology, more powerful and flexible, and less sensitive to variations in the cable and environment.
DNA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA. It accomplishes this by creating phosphodiester bonds between the 3' end of one nucleotide and the 5' end of the next nucleotide.
LIG4 DNA ligase 4 [ (human)] Gene ID: 3981, updated on 29-Jan-2017. The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ).