splicing, is currently the most commonly used fusion splicing technology. It is more expensive for its complex technology, more powerful and flexible, and less sensitive to variations in the cable and environment.
DNA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA. It accomplishes this by creating phosphodiester bonds between the 3' end of one nucleotide and the 5' end of the next nucleotide.
1 The gaps where the primer(s) were are then filled by yet more complementary nucleotides. 2  The new strand is proofread to make sure there are no mistakes in the new DNA sequence.  Finally, an enzyme called DNA ligase seals up the sequence of DNA into two continuous double strands.