splicing, is currently the most commonly used fusion splicing technology. It is more expensive for its complex technology, more powerful and flexible, and less sensitive to variations in the cable and environment.
DNA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA. It accomplishes this by creating phosphodiester bonds between the 3' end of one nucleotide and the 5' end of the next nucleotide.
It is now time to put the p53 gene in the plasmid. Another enzyme, called ligase, assists in the formation of bonds between adjacent, matching DNA ends. Your tape will play the role of the ligase. Insert the p53 gene in the plasmid DNA in the appropriate place.