splicing, is currently the most commonly used fusion splicing technology. It is more expensive for its complex technology, more powerful and flexible, and less sensitive to variations in the cable and environment.
DNA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA. It accomplishes this by creating phosphodiester bonds between the 3' end of one nucleotide and the 5' end of the next nucleotide.
DNA Helicase-The enzyme responsible for separating the two strands of DNA in a helix so that they can be copied during DNA replication. DNA Ligase-The enzyme responsible for sealing together breaks or nicks in a DNA strand.