1 I don't know what 10 year old boys are into anymore; my advice is to ask him what we wants for the cake or cupcakes. 2  If it's a surprise (or if he doesn't care as long... 3  10 is a hard age for cake because he's getting big, but I've had some recent luck impressing some 13 year old boys. 10 is a hard age for cake because he's getting big, but I've had some recent luck impressing some 13 year old boys. 2  You can see them on my myspace page (you donât h... 3  First, Pump it up is a great place. 4  My daughter celebrated her 6th birthday there.
Negative Staining- Principle, Reagents, Procedure and Result 3.95/5 (38) Negative Staining- Principle, Reagents, Procedure and Result. The main purpose of Negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. eg: Spirilla. It can also be used to stain cells that are too delicate to be heat-fixed.
1 Coomassie blue-stains proteins a brilliant blue, and is often used in gel electrophoresis. 2  Crystal violet-stains cell walls purple when combined with a mordant. 3  This stain is used in Gram staining. 4  DAPI-a fluorescent nuclear stain that is excited by ultraviolet light, showing blue fluorescence when bound to DNA. Hematoxylin-a nuclear stain that, with a mordant, stains nuclei blue-violet or brown. 2  Hoechst stains-two types of fluorescent stains, 33258 and 33342, these are used to stain DNA in living cells. 3  Iodine-used as a starch indicator. 4  When in solution, starch and iodine turn a dark blue color.