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BioFiles 2006, 1.4, 17. Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere with the processes at the 30S subunit or 50S subunit of the 70S bacterial ribosome. The aminoacyltRNA synthetases that activate each amino acid required for peptide synthesis are not antibiotic targets.