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Put the following steps of Koch's postulates in order: a. The suspected infectious agent must be isolated and grown outside the host. b. The suspected infectious agent causes the disease when it is introduced to a healthy, experimental host. c. The suspected infectious agent must be found in every case of the disease.
Leaf spot pathogens infect through natural plant openings such as stomates or by penetrating directly through the host cuticle and epidermal cell wall. In order to penetrate directly, fungi produce hydrolytic enzymesâcutinases, cellulases, pectinases and proteasesâfor breaking down the host tissue.