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About the time of birth in mammals, a secondary ossification center appears in each end (epiphysis) of long bones. Periosteal buds carry mesenchyme and blood vessels in and the process is similar to that occurring in a primary ossification center.
What is the second stage of Intramembranous Ossification. Primary center of ossification has osteoprogenitor cells that give rise to osteoblasts that lay down organic bone matrix by the alkaline phosphatase enzyme that allows calcification and this leads to imprisonment of osteoblast, making them osteocytes. What is the third stage of Intramembranous Ossification.