Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
The Eastern zone is one hour ahead of the Central zone. If it's 5:00 P.M in the CST zone, then it is 6:00 P.M in the EST zone. Always add one hour to the Central time to convert to the Eastern one. Both of these locations observe Daylight Saving Time, therefore there is a period of each year from March until November where the clocks are moved forward an hour.