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Mannitol Injection USP is one of the nonelectrolyte, obligatory, osmotic diuretics. It is freely filterable at the renal glomerulus, only poorly reabsorbed by the renal tubule, not secreted by the tubule, and is pharmacologically inert.
After the addition the nitrate reagents the liquid turned from yellow to red, indicating a positive result. This test eliminated E. coli, leaving the others showing a positive reaction. The mannitol test was used to show if a bacterium could ferment mannitol. For this test, I was able to finalize the result of my unknown to be Proteus vulgaris.