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Ethylene Oxide sterilization is a chemical process consisting of four primary variables: gas concentration, humidity, temperature and time. Ethylene oxide is an alkylating agent that disrupts the DNA of microorganisms, which prevents them from reproducing.
Ethylene oxide is still widely used by medical device manufacturers . Since ethylene oxide is explosive at concentrations above 3%, ethylene oxide was traditionally supplied with an inert carrier gas such as a CFC or HCFC. The use of CFCs or HCFCs as the carrier gas was banned because of concerns of ozone depletion.