Detects therapeutic and illicitly synthesized forms of acetyl fentanyl and fentanyl. Useful in many situations: pain management, dependency/ rehabilitation, methadone clinics, and drug courts. Fentanyl is screened via enzyme immunoassay (EIA). Acetyl fentanyl and fentanyl are confirmed on GC-MS equipment.
To use this method, make a diluted bleach solution with one part bleach and one to two parts water. Fill your plastic container with the solution and let it soak for about half an hour. After itâs done soaking in the solution, give the container a good scrubbing with hot water and dish soap.
When you begin handling the Catechol solution you will need to use gloves and safety glasses or goggles as it is a poison with a splash hazard. Report all spills to your instructor immediately. Any solution contaminated with catechol must be disposed of in the toxic waste beaker in the hood. Mix 1 mL of the supernatant (crude extract) with 19 mL of ice cold deionized water (see bottle in refrigerator) to make 20 mL of dilute extract. 2  This will be your enzyme solution so keep it on ice. 3  Set vortexer to touch and the speed to between 5 & 6.