Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
The Eastern Time Zone (ET) is a time zone encompassing 17 U.S. states in the eastern part of the contiguous United States, parts of eastern Canada, the state of Quintana Roo in Mexico, Panama in Central America and the Caribbean Islands. Alabama: The entire state is officially in the Central Time Zone. 2  However, a handful of communities unofficially observe Eastern Time because they are part of the Columbus, Georgia metropolitan area â Phenix City, Smiths Station, Lanett, and Valley.