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For Eukaryotes the electron transport chain occurs in the Inner Membrane of a mitochondria. (mitochondria has 2 membranes both outer and inner). For Prokaryotes, it occurs in the plasma membrane as prokaryotes do not have mitochondria.
The electron transport chain is located in the cristae of the inner mitochondrial membrane. The NADH and FADH 2 produced by the citric acid cycle in the matrix release a proton and electron to regenerate NAD + and FAD.