Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
Converting EST to MST. This time zone converter lets you visually and very quickly convert EST to MST and vice-versa. Simply mouse over the colored hour-tiles and glance at the hours selected by the column... and done! EST stands for Eastern Standard Time. MST is known as Mountain Standard Time. MST is 2 hours behind EST.