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For Eukaryotes the electron transport chain occurs in the Inner Membrane of a mitochondria. (mitochondria has 2 membranes both outer and inner). For Prokaryotes, it occurs in the plasma membrane as prokaryotes do not have mitochondria.
In eukaryotes, an important electron transport chain is found in the inner mitochondrial membrane where it serves as the site of oxidative phosphorylation through the use of ATP synthase. It is also found in the thylakoid membrane of the chloroplast in photosynthetic eukaryotes.n mitochondria, it is the conversion of oxygen to water, NADH to NAD + and succinate to fumarate that are required to generate the proton gradient. Electron transport chains are major sites of premature electron leakage to oxygen, generating superoxide and potentially resulting in increased oxidative stress.