Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
EST is the Eastern standard time and is 5 hours behind the GMT. EDT vs EST. EDT and EST are parts of the Eastern Time Zone that is observed in 17 states of the country lying in the eastern region. In the northern parts of this time zone, clocks are turned back by an hour during spring to save daylight.