Eluted DNA for each kit was pooled, and DNA concentration was determined using spectrophotometry and adjusted to 17.5 ng/Î¼L. The purified DNA (350 ng) underwent bisulfite conversion using either the MethylEdgeâ¢ Bisulfite Conversion System, kit A or kit B according to the manufacturers' instructions (n = 3 for each kit).
â¢ The time difference between Mountain Time and Eastern Time is two hours. â¢ Mountain Time and Eastern Time are two standard time zones in North America. â¢ The Mountain Time zone is ahead of the Pacific Time Zone by one hour and is behind the Central Time Zone by one hour.
â¢ There is 3 hours of time difference between Pacific Time and Eastern Time. â¢ Some states in United States belong to both time zones. For example. Washington D.C belongs to Eastern Time while Washington belongs to Pacific Time. â¢ Eastern Time in US and Canada. Eastern Standard Time (EST) = UTC â 5. Eastern Daylight Time (EDT) = UTC â 4