Preparation of MacConkey Agar. 1  Suspend the measured amount of powder (See in the agar bottle and generally 50 gram) in 1 L of purified water and mix thoroughly. 2  Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3  Autoclave at 121Â°C for 15 minutes.
All USDA loans (purchase and refinance) require a one-time Guarantee Fee that is added to the borrowers loan. The current amount of the USDA guarantee fee is 2%. Example â if a borrower was purchasing a home 100% financing for $150,000, the adjusted loan amount with the 2% USDA guarantee fee would be $153,000. $3,000 added to the base loan amount. In addition to this one time Guarantee Fee, USDA loans also require a annual fee that is added to the monthly mortgage payment. Basically the âannual feeâ is monthly mortgage insurance â or more commonly known as âPMIâ Again, like the above it can have many different names. The current amount of this fee is .5%.
USDA Purchase & Refinance Transactions: new fee effective October 1st, 2014 .50% (up from .40%). An example of how the annual fee is calculated. For a loan amount of $153,000, the new annual fee would be .50%, or $153,000 x .05 = $765.00 divided by 12 for a monthly figure of $63.75 per month.he upfront USDA Guarantee Fee for all new Florida USDA 502 Guarantee purchase and refinance loans as of October 1 2014 â October 1 2015 will be as follows: