I am doing some monitoring with prometheus and is trying to understand how to properly use the rate functions. Premise is this; I have a counter, configuration for this is set to ingest new values...
Enzymes. Enzymes are proteins, produced by living cells; they catalyze chemical reactions by lowering the activation energy. Activation energy is the energy required for a chemical reaction to occur. Enzymes are generally globular proteins with characteristic three-dimensional shapes.The active site is the binding site for the enzymeâs substrate. Enzymes are specific for substrate molecules.ctivation energy is the energy required for a chemical reaction to occur. Enzymes are generally globular proteins with characteristic three-dimensional shapes. The active site is the binding site for the enzymeâs substrate. Enzymes are specific for substrate molecules.
DNA Ligase, T4. The catalytic activity of the enzyme requires the presence of ATP and Mg++. DNAs that lack the required phosphate residues can be rendered capable of ligation by phosphorylation with T4 polynucleotide kinase. The enzyme also catalyzes an addition reaction of phosphate between pyrophosphate and ATP.