Preparation of MacConkey Agar. 1  Suspend the measured amount of powder (See in the agar bottle and generally 50 gram) in 1 L of purified water and mix thoroughly. 2  Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3  Autoclave at 121Â°C for 15 minutes.
All USDA loans (purchase and refinance) require a one-time Guarantee Fee that is added to the borrowers loan. The current amount of the USDA guarantee fee is 2%. Example â if a borrower was purchasing a home 100% financing for $150,000, the adjusted loan amount with the 2% USDA guarantee fee would be $153,000. $3,000 added to the base loan amount. In addition to this one time Guarantee Fee, USDA loans also require a annual fee that is added to the monthly mortgage payment. Basically the âannual feeâ is monthly mortgage insurance â or more commonly known as âPMIâ Again, like the above it can have many different names. The current amount of this fee is .5%.
The one-time fee is added to the total balance of the mortgage and financed into the loan. On Oct.1, that 3.5 percent fee will decrease to 2 percent. But the USDA will implement a new annual fee of 0.3 percent of the balance of the loan. This fee will be added to the monthly payments.Refinancers will have to pay the annual 0.3 percent fee, plus the current one-time upfront guarantee fee of 1 percent of the total of the mortgage.The upfront fee will stay unchanged for refinances.f you are refinancing: Refinancers will have to pay the annual 0.3 percent fee, plus the current one-time upfront guarantee fee of 1 percent of the total of the mortgage. The upfront fee will stay unchanged for refinances.