Muse, Muses, Of Song. Muse with barbiton, Paestan red-figure lekanis C4th B.C., MusÃ©e du Louvre. THE MOUSAI (Muses) were the goddesses of music, song and dance, and the source of inspiration to poets. They were also goddesses of knowledge, who remembered all things that had come to pass.
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.
1 The extension time depends both on the DNA polymerase used and on the length of the DNA fragment to amplify. 2  As a rule-of-thumb, at its optimum temperature, the DNA polymerase polymerizes a thousand bases per minute. Miniprimer PCR: uses a thermostable polymerase (S-Tbr) that can extend from short primers (smalligos) as short as 9 or 10 nucleotides. 2  This method permits PCR targeting to smaller primer binding regions, and is used to amplify conserved DNA sequences, such as the 16S (or eukaryotic 18S) rRNA gene.