Muse, Muses, Of Song. Muse with barbiton, Paestan red-figure lekanis C4th B.C., MusÃ©e du Louvre. THE MOUSAI (Muses) were the goddesses of music, song and dance, and the source of inspiration to poets. They were also goddesses of knowledge, who remembered all things that had come to pass.
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.
Since PCR is, basically, replication of DNA in a test-tube, all the usual ingredients needed for DNA replication are required: A template (the DNA that is being copied) Primers (to initiate the synthesis of the new DNA strands) DNA polymerase (to carry out the synthesis) dNTPs (nucleotide precursors). Cycling temperatures: Of the three temperatures used in a cycle of PCR, the denaturing and extension temperatures remain unchanged for the most part, since most DNA is denatured at 94-95 degrees C, and the temperature optimum for the Taq DNA polymerase lies between 72 and 74 degrees C.