28598 County Road 4, Pequot Lakes, MN 56472 View this property at 28598 County Road 4, Pequot Lakes, MN 56472
CRISPR/Cas9, a gene-editing technique that can target and modify DNA with groundbreaking accuracy, is both the newest darling and the newest villain of genetics research.Invented in 2012 by scientists at the University of California, Berkeley, CRISPR/Cas9 has received a lot of attention this year.f scientists are able to nail down the use of CRISPR/Cas9 in human germ cells, thereâs no question that it could confer major benefits. Namely, the technology could eradicate hereditary diseases such as cystic fibrosis, sickle-cell anemia, and Huntingtonâs disease from a family line altogether.
CRISPR is a bacterial system that bacteria use to fight viruses. It consists of an enzyme called Cas9 and a guiding RNA. Cas9 works together in a complex with the guide RNA to be directed to the complementary sequence of a gene that is being targeted where a ds break will be generated. Target sequence must have a PAM sequence, which is just a 3 nt sequence and varies per bacteria.