We donât eat a lot of bacon, but when we do I try to use turkey bacon. Lean, lean, lean turkey bacon. But the problem with the âlean-nessâ is that it doesnât release much fat to cook itself, like pork bacon does. Iâve tried cooking turkey bacon in the microwave on paper towels.
But from another perspective library preparation is anything but straightforward. Starting from total RNA, say you are interested in microRNA (which is a somewhat outdated term now, âsmall RNAâ or ânon-coding RNAâ are better descriptive terms). How does that get converted to cDNA with synthetic adapters?
But extracting that particular DNA sequence or gene of interest from the whole lot of DNA sequences present in the library is quite a challenging task which is overcome by the development and application of various screening procedures and screening a genomic library and cDNA library have specific protocols.