Muse, Muses, Of Song. Muse with barbiton, Paestan red-figure lekanis C4th B.C., MusÃ©e du Louvre. THE MOUSAI (Muses) were the goddesses of music, song and dance, and the source of inspiration to poets. They were also goddesses of knowledge, who remembered all things that had come to pass.
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.
The following example outlines how to calculate the volumes of primers and probe needed such that the primers and probe will be at a desired final concentration in a 5â nuclease reaction. This example is presented simply to demonstrate how to dilute TaqManÂ® primers and probes in a PCR reagent cocktail.he following example demonstrates how to dilute probes to a desired working stock concentration. A common range of probe working stock concentration is from 2 Î¼molar to 10 Î¼molar. Example: Diluting already reconstituted TaqManÂ® probe with a concentration of 100 Î¼M to a working stock concentration of 10 Î¼M.