Muse, Muses, Of Song. Muse with barbiton, Paestan red-figure lekanis C4th B.C., MusÃ©e du Louvre. THE MOUSAI (Muses) were the goddesses of music, song and dance, and the source of inspiration to poets. They were also goddesses of knowledge, who remembered all things that had come to pass.
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.
In the presence of Mn 2+ , Tfl DNA polymerase can use RNA as a template. Tfl DNA polymerase exhibits a 5â²â3â² exonuclease activity but lacks a 3â²â5â² exonuclease activity. This enzyme is commonly used in PCR (Gaensslen et al. 1992), where its activity is similar to that of Taq DNA polymerase.Tfl DNA polymerase is used in the Access and AccessQuickâ¢ RT-PCR Systems.aq DNA polymerase is suitable for most PCR applications that do not require a high-fidelity enzyme, such as detecting specific DNA or RNA sequences. The error rate of Taq DNA polymerase is approximately 1 Ã 10 â5 errors/base, although the fidelity does depend somewhat on the reaction conditions.