Muse, Muses, Of Song. Muse with barbiton, Paestan red-figure lekanis C4th B.C., MusÃ©e du Louvre. THE MOUSAI (Muses) were the goddesses of music, song and dance, and the source of inspiration to poets. They were also goddesses of knowledge, who remembered all things that had come to pass.
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.
Aliquot 22.75 Âµl of the PCR Master Mix without Taq Polymerase to the No EnzymeControls.10. Add Taq Polymerase (0.25 Âµl x number of remaining reactions) to the PCR âMasterMixâ and mix. Aliquot 23Âµl of the mixture into tubes containing experimental samplesand Control Template dilutions.