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Penicillin Mode of Action - Enzyme Inhibition: All penicillin derivatives produce their bacteriocidal effects by inhibition of bacterial cell wall synthesis. Specifically, the cross linking of peptides on the mucosaccharide chains is prevented.
Mechanism of Action. â¢ Penicillins are dipeptide analogs of D-ala-D-ala. â¢ Radiolabelled Penicillin G binds to several different proteins in the cell wall of bacteria (Table 1). â¢ Bactericidal action is primarily due to inhibition of PBPs 1a & 1b and PBP3 that function as. transpeptidases in the biosynthesis of the peptidoglycan.