Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Iowa's sales tax weekend is Friday and Saturday, August 6th and 7th. Illinois' is from August 6th through the 15th. The Illinois holiday does include school supplies, which Iowa's tax-free weekend does not. Also a good summary of all state tax holidays here.