Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Missouri's Tax-Free Weekends. Save Money During Missouri's Sales Tax Holidays. Each year, the state of Missouri offers at least one three-day tax holiday intended for back-to-school buyers. In some years, it also runs a Show Me Green tax holiday for purchases related to energy conservation.