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Starch Hydrolysis Medium. Purpose: To determine if a bacterium produces the enzyme amylase, which breaks down starch to glucose. Principle: Starch Hydrolysis medium is a simple medium that contains beef extract, soluble starch and agar.Because starch is such a large molecule it cannot be directly transported into a bacterial cell.tarch Hydrolysis Medium. Purpose: To determine if a bacterium produces the enzyme amylase, which breaks down starch to glucose. Principle: Starch Hydrolysis medium is a simple medium that contains beef extract, soluble starch and agar.
Some starches, for instance from potato, contain covalently bound phosphate in small amounts (0.2% approximately), which has significant effects on the physical properties of the starch but does not interfere with its hydrolysis. Acid hydrolysis of starch has had widespread use in the past.