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The spectral sensitivity is typically expressed as a QE Curve, in which the probability to detect a photon of a particular wavelength is expressed as a percentage.So for example if one in every 10 photons is detected this is expressed as a QE of 10%. The curve for a typical CCD is shown in the diagram on the below:
In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. double beam spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample. A single-beam spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted.