Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
2017 ARKANSAS SALES TAX HOLIDAY. Arkansas will hold its annual sales tax holiday, beginning Saturday, August 5, 2017 at 12:01 a.m. and ending. Sunday, August 6, 2017 at 11:59 p.m. State and local sales tax will not be collected during this 48-hour period on. the sale of: (1) Clothing and footwear if the sales price is less than one hundred dollars ($100) per item; (2) Clothing. accessories and equipment if the sales price is less than fifty dollars ($50) per item; (3) School supplies; (4) School.