Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Oklahoma Sales Tax Holiday 2016. The state of Oklahoma is offering one tax free weekend event during 2016. It applies to clothing and shoes and is intended to coincide with back-to-school shopping, when many parents stock up on kidsâ clothing and footwear. The Oklahoma Tax Commission has announced the dates for the Oklahoma Sales Tax Holiday 2016.