Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Ohio to have sales tax holiday in August! Ohio is going to have a sales tax holiday this August, just in time for back-to-school! The holiday starts on Friday, August 5, 2016 at 12:01 a.m. and ends on Sunday, August 7, 2016 at 11:59 p.m. During this time only, you can buy certain items and not pay any sales or use tax.