Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Sales Tax Holiday. The state of Tennessee's annual sales tax holiday is held every year, beginning at 12:01 a.m. on the last Friday in July and ending at 11:59 p.m. the following Sunday night. During this weekend, certain goods may be purchased tax free. This year's tax-free holiday weekend begins at 12:01 a.m. on Friday, July 28 and ends Sunday, July 30 at 11:59 p.m.