Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Missouri Tax-Free Shopping Weekend. If you want to stay informed of St. Louis information, subscribe to the Arch City Homes weekly email so you donât miss any posts! In order to help ease the Back to School spending burden on Missouri families, the state offers an annual tax-free shopping weekend the 1st weekend in August.