Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Texas' Tax-Free Weekend 2016. Find out everything you need to know about the Texas sales tax holiday August 5-7, 2016. Everythingâs bigger in Texas, and that includes the upcoming sales tax holiday. From August 5-7, Texas shoppers can say adios to about 8% sales tax on most clothing, footwear, backpacks, and other school supplies priced at $100 or less.