Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Iowa Sales Tax Holidays. Iowa Sales Tax Holidays Ken Colborn. A sales tax holiday is a specific period of time during which select goods are exempt from state sales and use tax. These tax-free periods may or may not extend to local sales and use taxes in addition to state sales and use tax.