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ATR generally allows qualitative or quantitativeanalysis of samples with little or no sample preparation, whichgreatly speeds sample analysis. The main beneï¬t of ATR samplingcomes from the very thin sampling pathlength and depth of penetration of the IR beam into the sample.
Because unrelated people will almost definitely differ in their number of repeats, STRs can be used to confidently discriminate between unrelated persons. DNA is isolated from material collected at the crime scene. The STR loci are amplified by PCR using sequence-specific primers. These primers are designed in such a way that they can amplify the STR loci from any individual. The resulting DNA fragments are then processed using electrophoresis.