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In vivo, chymotrypsin is a proteolytic enzyme (Serine protease) acting in the digestive systems of many organisms. It facilitates the cleavage of peptide bonds by a hydrolysis reaction, which despite being thermodynamically favorable occurs extremely slowly in the absence of a catalyst.
[edit]. Î±-chymotrypsin. The labelled amino acids form the active site of the enzyme. 1  Enzymes are protein macromolecules. 2  They have a defined amino acid sequence, and are typically 100-500 amino acids long. 3  They have a defined three-dimensional structure. Enzymes are protein macromolecules. 2  They have a defined amino acid sequence, and are typically 100-500 amino acids long. 3  They have a defined three-dimensional structure. 4  Enzymes are catalysts. 5  They act as a catalyst to a chemical or biochemical reaction, with a defined mechanism.