Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
How 2017 Sales taxes are calculated in Missouri. The state general sales tax rate of Missouri is 4.23%. Cities and/or municipalities of Missouri are allowed to collect their own rate that can get up to 4.45% in city sales tax. Every 2017 combined rates mentioned above are the results of Missouri state rate (4.23%), the county rate (0% to 3.13%), the Missouri cities rate (0% to 4.45%), and in some case, special rate (0% to 2%).