Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Beginning at 12:01 a.m. on Saturday August 5, 2017, and ending at 11:59 p.m. on Sunday August 6, 2017, the State of Arkansas will hold its sales tax holiday allowing shoppers the opportunity to purchase certain School Supplies, School Art Supplies, School Instructional Materials, and clothing free of state and local sales or use tax.