A good rule of thumb is: 1  for difficult separations (Î´CV â¤ 1) use up to 100 g (~200 mL) of silica gel per gram of compound to be purified (1% load) 2  for normal separations (1 â¤ Î´CV â¤ 5) use from 20 to 90 g (~40-180 mL) of silica gel per gram of compound to be purified (2-9% load)
Typical costs: 1  According to the Academy of Managed Care Pharmacy , insulin pumps cost between $4,500 and $6,500 for individuals without insurance. The price varies depending upon the features, brand and size of the pump. 2  Some insurance plans will cover the cost of the pump.
Protamine insulin was first created in 1936 and NPH insulin in 1946. It is on the World Health Organization's List of Essential Medicines, the most important medications needed in a basic health system. The wholesale cost in the developing world is about 2.23 to 10.35 USD per 1,000 iu of NPH insulin.