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A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.It is mainly associated with retroviruses.elomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
cDNA Synthesis & RT-PCR. The ability to synthesize DNA from an RNA template, via reverse transcription, enables researchers to study RNA with the same molecular approaches used for DNA investigations.cDNA generated by reverse transcription can be amplified using polymerase chain reaction (PCR).he ability to synthesize DNA from an RNA template, via reverse transcription, enables researchers to study RNA with the same molecular approaches used for DNA investigations. cDNA generated by reverse transcription can be amplified using polymerase chain reaction (PCR).