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The enzyme activity gradually increases with temperature up to around 37ÂºC, or body temperature. Then, as the temperature continues to rise, the rate of reaction falls rapidly as heat energy denatures the enzyme.Graph of enzyme activity against temperature.s the temperature increases, so does the rate of reaction. But very high temperatures denature denature: disable by changing the normal qualities or nature of something enzymes. The graph shows the typical change in an enzyme's activity with increasing temperature.
pH affect enzyme activity. . it leads to alteration in the shape of the enzyme.is known as the optimum pH. the pH level may also affect the charge properties and shape of the substrate.This is graphically illustrated in Figure 2.the point where the enzyme is most active.H affect enzyme activity. . it leads to alteration in the shape of the enzyme.is known as the optimum pH. the pH level may also affect the charge properties and shape of the substrate.