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A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.It is mainly associated with retroviruses.elomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
Summary: cDNA stands for complementary DNA or copy DNA and can be single-stranded or double-stranded. cDNA is synthesized in vitro from a mRNA template using reverse transcriptase.The resulted cDNA is single-stranded. This process is called reverse transcription (RT) or first strand cDNA synthesis.In some experiments such as microarray, double-stranded cDNA is required and can be produced by another round of DNA synthesis following the first strand synthesis.he resulted cDNA is single-stranded. This process is called reverse transcription (RT) or first strand cDNA synthesis. In some experiments such as microarray, double-stranded cDNA is required and can be produced by another round of DNA synthesis following the first strand synthesis.