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Temperature in the range of 40â50Â°C did not affect enzyme activity. T. polysporum was found as the most promising strain to produce chitosanase with maximal enzyme activity of about 1.4 IU/gds, followed by T. viride (~1.2 IU/gds) and T. harzianum (1.06 IU/gds).emperature in the range of 40â50Â°C did not affect enzyme activity. T. polysporum was found as the most promising strain to produce chitosanase with maximal enzyme activity of about 1.4 IU/gds, followed by T. viride (~1.2 IU/gds) and T. harzianum (1.06 IU/gds).
This was received at pH 2. The pH has an affect on enzymes as the three-dimensional shape that enzymes molecular structure is based on, is partly the result of hydrogen bonding. PH, which is a measure of hydrogen ion concentration, will affect the enzyme by changing the concentration of hydrogen in that enzyme.Any change in pH can effectively denature enzymes. My anomalous results were identified and a justification for the reason behind it was explained.ther variable like temperature, enzyme concentration, substrate concentration, the affect of competitive and non-competitive inhibitors are all possibilities on the affect of enzymes. Concluding my investigating, my results support my hypothesis. At a pH level either too high or too low level would denature the enzyme.