Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
385 EXPERIMENT 24 Amplification of a DNA Fragment Using Polymerase Chain Reaction Theory Polymerase chain reaction (PCR) is a techniquethat allows the amplification of a specific fragmentof double-stranded DNA in a matter of hours.ny biological sample recovered from a crime scenethat contains DNA (a single hair, a drop of blood,skin cell, saliva, etc.) can be subjected to PCR.Primers complementary to repetitive DNA se-quences found throughout the human genome arechange a single base pair in the gene of interest(Fig. 24-4 d).