Curley + Associates (GMCA), and OMSI, this unique and interactive experience showcases areas of forensic science that enabled Sherlock Holmes to solve crimes, as well as the historic underpinnings of author Sir Arthur Conan Doyle's rich and vibrant stories.
Many substances absorb light. However some of them, after absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy. Such substances are called âfluorescent substancesâ. Application of this phenomenon is the basis of fluorescence microscope.
The emission filter further screens the light, the emitted light is measured by the detector, and the fluorescence value is displayed on the instrument. Figure 2 illustrates the key components of a filter fluorometer: light source/lamp; excitation and emission filters; sample cell/cuvette; and light detector.or example, if the substance is linear to 250 ppm and measurable with a calibration curve to 1000 ppm, you might calibrate with a 200 ppm standard and take readings at 500, 750, and 1000 ppm for a calibration curve. In some cases, calibration with a standard of known concentration is not necessary.