If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Missouri Energy Star Sales Tax Holiday 2016. Section 144.526.1 creates the âShow-Me Green Sales Tax Holidayâ or the Missouri Energy Star Sales Tax Holiday. Beginning in 2009, during a seven day period starting on April 19th and ending April 25th, sales of qualifying Energy Star certified new appliances will be exempt from state sales tax.