If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Top 7 Missouri Festivals and events not to miss in 2017. 1  Northwest Missouri State Fair 2017. 2  Missouri Music Festival. 3  Apple Butter Festival.  Missouri Bluegrass 1  festival. Mosaics Festival For the Arts in Missouri.  Missouri Renaissance 1  Festival. Washington County MO State  Fair.