Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
DNA polymerase uses the complementary nature of DNA strands to replicate DNA. The polymerase enzyme does not contain any information about the order of base sequences. Without a template strand of DNA the enzyme is incapable of selecting which base to add to the sequence next. DNA polymerase binds to a DNA strands and adds new material to it in order to build a complementary strand. The complementary nature of the four bases provides the information to build the correct sequence guanines, cytosines, adenines, and thymines.