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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
Copying occurs at a localized region called the replication fork, which is a Y shaped structure where new DNA strands are synthesised by a multi-enzyme complex. Here the DNA to be copied enters the complex from the left.One new strand is leaving at the top of frame and the other new strand is leaving at bottom.The first step in DNA replication is the separation of the two strands by an enzyme called helicase.he other half of the DNA double helix, known as the lagging strand, has the opposite 3' to 5' orientation and consequently requires a more complicated copying mechanism. As it emerges from the helicase, the lagging strand is organised into sections called Okazaki fragments.