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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
Explain how the lagging strand is synthesized even though DNA polymerase can add nucleotides only to the 3' end. As replication bubble opens, polymerase molecule work its way away from replication fork and synthesize a short segment of DNA. DNA ligase enzyme that joins with sugar phosphate backbones of Okazaki fragments to create DNA strand