Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
The standard PCR enzymes are not surprisingly quite accurate. Even Thermus aquaticus (Taq) DNA polymerase, a low fidelity PCR polymerase, only makes a mistake once in approximately 100,000 nucleotide insertions, depending on reaction conditions (4).The basis of polymerase accuracy is an exciting area of investigation.uring PCR, Taq DNA Polymerase will misincorporate nucleotides at a particular rate leading to mismatch formation. This is termed âerror rateâ. Taq, and polymerases in general, will stall at these mismatched bases and are more likely to dissociate before extending as compared to correctly base paired 3Â´ ends.