If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Tax-free holiday in Missouri this weekend. This is an archived article and the information in the article may be outdated. Please look at the time stamp on the story to see when it was last updated. (KTVI) â The Missouri Sales-Tax Holiday is this weekend, August 1-3. That means sales tax on certain back-to-school products will be waived. Eligible items include clothing, footwear, computers and traditional school supplies. The tax waiver includes online orders as well.