If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Missouri's state flag was chosen to be Missouri's state flag on March 22, 1913. The flag was designed by Marie Elizabeth Oliver. The flag has one red stripe, one blue stripe, and one white stripe. The flag has a picture of Missouri's state seal on it. This flag is not the original flag though the real flag is at the secretary of offices in Jefferson city. Miss ouri's state flow er is the White Hawthorn Blossom. It be came Missouri's state flower on March 16, 1923. This flower grows in bunches. It is mostly found in Southern Missouri.