Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
But these thermophile DNA polymerases work at 100C, a temperature at which DNA is denatured (in linear form). This thermophilic DNA polymerase is called Taq polymerase, named after T hermus aq uaticus, the bacteria it is derived from.Taq polymerase, however, has no proofreading ability. Other thermally stable polymerases, such as Vent and Pfu, have been discovered to both work for PCR and to proofread.his is the enzyme that is in charge of replicating DNA. This is the polymerase part of the name polymerase chain reaction. 4. Nucleotides. You'll need to add nucleotides (dNTPs) so the DNA polymerase has building blocks to work with.