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What is the best time to take Insulin? The best time to take ordinary insulin is 30 minutes before food, so the level of insulin and sugar in the blood after food intake matches . But in case you have eaten already, it is better to take the injection immediately after, rather than not taking.
Often, you use an insulin that has about a 12 hour duration. This way you can give 2 shots a day and not have the activity from the first dose overlap the activity of the second dose. During each of these 12 hour cycles, the insulin will have an onset, peak, and duration. All these times depend on your pet and on the type of insulin you are using. With some pets, the insulin duration is close to 24 hours, so only one shot is given each day.