Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
Such is the history of Taq DNA polymerase. The original report of this enzyme, purified from the hot springs bacterium Thermus aquaticus, was published in 1976. Roughly 10 years later, the polymerase chain reaction was developed and shortly thereafter Taq became a household word in molecular biology circles.Currently, the world market for Taq polymerase is in the hundreds of millions of dollars each year.s would be expected from first principles, The total error rate of Taq polymerase has been variously reported between 1 x 10 -4 to 2 x 10 -5 errors per base pair. Pfu polymerase appears to have the lowest error rate at roughly 1.5 x 10 -6 error per base pair, and Vent is probably intermediate between Taq and Pfu.