The theory goes that these weren't normal rocks but rather the equivalent of cosmic sponges, loaded with water that was released on impact. While astronomers have confirmed that some asteroids and comets hold water, some scientists think the theory doesn't.
Western blot using an antibody that recognizes proteins modified with lipoic acid. The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract.It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide.he name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern.
WESTERN BLOTTING TIPS AND TROUBLESHOOTING GUIDE TIPS FOR SUCCESSFUL WESTERB BLOTS TROUBLESHOOTING GUIDE 1. Suboptimal protein transfer. This is the most common complaint with western blotting and could be due to many different problems listed below. a) Low quality membrane used for transfer. High quality PVDF membrane is the blotting membrane of choice as low molecular weight proteins are sometimes transferred through nitrocellulose membranes and therefore may be not visible on the blot. b) Improper Western transfer procedure. c) For semi-dry Western transfers make sure the filter paper is wet.