If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Local jurisdictions can choose to participate in the Show-Me Green Sales Tax Holiday. tax free weekend april 2016 missouri. Missouri Tax Free Weekend, August 5-7, 2016. Starting today, shoppers will be out in droves to purchase everything from pencil cases to laptops during the annual Missouri tax-free weekend.