Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
The job of Taq polymerase is to move along the strand of DNA and use it as a template for assembling a new stand that is complimentary to the template. This is the chain reaction in the name polymerase chain reaction. PCR is so efficient because it multiplies the DNA exponentially for each of the 25 to 75 cycles.his is the polymerase part of the name polymerase chain reaction. 4. Nucleotides. You'll need to add nucleotides (dNTPs) so the DNA polymerase has building blocks to work with. There are three major steps to PCR and they are repeated over and over again, usually 25 to 75 times.