The steps of using recombinant DNA technology are: 1. isolation and cutting of DNA of required gene 2. introduce cut DNA into a vector 3. amplification of gene with the â¦ help of PCR 4. introduce into compotetant host 5. obtaning gene product 6. down processing. 20 people found this useful.
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In smaller e-cigs, it is not uncommon for the filler material to be recessed inside the cartridge. In this case, take a paper clip and insert it into the hole you would suck through on the cartridge. The inner cup will push up and you can hold it up higher where it is easier to drip liquid into it.