If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
February 2018 - Nixa, Missouri - Sunrise and sunset calendar. Sunrise and sunset times, civil twilight start and end times as well as solar noon, and day length for every day of February in Nixa, MO. In Nixa, MO, the first day of February is 10 hours, 24 minutes long. The last day of the month is 11 hours, 23 minutes, so the length of the days gets 58 minutes longer in February 2018. Â« January 2018