If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
The Missouri Compromise was an agreement between the North and the  South and passed by Congress in 1820 that allowed Missouri to be  admitted as the 24th state in 1821. One â¦ slave state (Missouri) and  one free state (Maine) were admitted to the Union, maintaining the  balance.  So the Compromise set a bunch of rules about slavery, but the big  thing it did was it separated Maine from Massachusetts and allowed  it to become a free state. Missouri was allowed to become a slave  state.