Additionally, Creon may be feeling quite defensive since he is in the wrong: the brothers fight and kill each other because Creon supports Eteocles' refusal to honor a legal, joint royal power-sharing arrangement with twin brother Polyneices.
Thermostable DNA polymerases that have a proofreading function, such as Pfu and Tli (a) Â® -T Vectors if they are first tailed with dATP using Taq DNA Polymerase (1). This A-tailing protocol can also be used to subclone DNA fragments generated by restriction enzymes which produce blunt ends (1).igure 2. Analysis of recombinants obtained after cloning of an A-tailed PCR fragment into the pGEM Â® -T Easy Vector. A 1,200bp fragment was generated by amplification with Pfu DNA polymerase. This fragment was tailed using the alternative procedure described in Figure 1B.
A nucleotide can be added to DNA polymerase only on a pre-existing 3â-OH group which requires a primer so that it may add to the nucleotide. The so called primers do contain DNA and RNA base. DNA has the base thymine while RNA has uracil as its base. nucleotide can be added to DNA polymerase only on a pre-existing 3â-OH group which requires a primer so that it may add to the nucleotide. The so called primers do contain DNA and RNA base. DNA has the base thymine while RNA has uracil as its base.