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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
DNA ligase is an enzyme which seals gaps in the sugar-phosphate backbone of DNA molecules. This is necessary in the replication of DNA as the lagging strand is synthesised i â¦ n short fragments by DNA polymerase. The gaps between the fragments are then sealed by DNA ligase.