The Cas9 nuclease and requirements for RNA-guided genome-editing. Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences (Deltcheva et al.2011). A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex (Jinek et al. 2012).he Cas9 nuclease and requirements for RNA-guided genome-editing. Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences (Deltcheva et al. 2011).
Solid Surface Bathroom Countertops: These countertops are made from synthetic materials, so they are manufactured in many colors and styles. The pros of solid surface countertops include the choices, strength and a non-porous surface that cleans up quickly.
How to Caulk a Bathroom Sink. Drop-in sinks are made to hang from the top of a counter by their rims. While these rims appear to sit flush to the counter, there is actually a small gap between the rim and counter where water and debris can collect.Caulk fills this gap to make your counter and sink watertight, while helping to eliminate any movement of the rim of the sink.To help prevent leaks or damage to your counter, seal and fill this gap with waterproof caulk whenever the old caulk begins to crack, discolor or peel. Ad.aulk fills this gap to make your counter and sink watertight, while helping to eliminate any movement of the rim of the sink. To help prevent leaks or damage to your counter, seal and fill this gap with waterproof caulk whenever the old caulk begins to crack, discolor or peel. Ad.