Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Meyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.eyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.
Generally Meyer Lemon Trees need water every one to two weeks. Leaves can be an indicator as to how your tree feels. If the leaves are drooping like theyâre too heavy for the branches, and turning dark brown or black the tree is getting too much water.