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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
DNA ligase adds on nucleotides in the usual 5' to 3' direction along the DNA strand. In addition to bridging gaps during the process of DNA replication, DNA ligase also works to do the same thing during DNA repair. When DNA is repaired, a portion of the DNA where the error or damage occurred is removed.New DNA is synthesized that is not damaged and contains the correct DNA sequence.NA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA.