Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Meyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.eyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.
Basics of Meyer Lemon Growing. Meyer lemon tree care includes finding the right location for your tree. Whether grown in a container or planted in the ground, Meyer lemon growing requires at least six hours of sunlight. In the hottest summer areas, morning sun and afternoon shade is best for growing Meyer lemons.asics of Meyer Lemon Growing. Meyer lemon tree care includes finding the right location for your tree. Whether grown in a container or planted in the ground, Meyer lemon growing requires at least six hours of sunlight. In the hottest summer areas, morning sun and afternoon shade is best for growing Meyer lemons.