Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Meyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.eyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.
When should I expect my Meyer lemons to be ripe? How will I know when they are ready to harvest? Meyer lemons ripen, between November and April. You probably noticed they flowered over a long period of time, and obviously the first fruit to set would be the ones that ripened in November. The fruit will change color as it becomes ripe. In commercial groves, a device that checks the sugar levels in the fruit is used. It costs too much for the homeowner, so our best option is to pick fruit and try it to check ripeness. Meyer lemons are much sweeter than conventional lemons.