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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
DNA ligase is responsible for joining together fragments of DNA. In DNA replication, after the primers are replaced by DNA Polymerase I, DNA ligase assists in the formation ph â¦ osphodiester bonds between the fragments.This is essential for creating one continuous strand.NA helicase unzip, or separate, a strand of DNA at positions  called origins. This means that the hydrogen bonds between  complementary base pairs are removed (DNA is dou â¦ ble stranded!).  When they separate double-stranded DNA into single strands, it  allows each strand to be copied (replication).