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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
primase An enzyme that creates the short primer sequences needed for the discontinuous replication of DNA. It is an RNA polymerase that synthesizes a short segment of RNA, consisting of 10 to 12 nucleotides, attached to the lagging strand of the unwound DNA molecule.rimase An enzyme that creates the short primer sequences needed for the discontinuous replication of DNA. It is an RNA polymerase that synthesizes a short segment of RNA, consisting of 10 to 12 nucleotides, attached to the lagging strand of the unwound DNA molecule.