Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Meyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.eyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.
Wait until the soil around your tree is dry to the touch before watering your Meyer lemon tree. The soil should be moist but not soggy. Do not wait until the ground is parched before watering as this can lead to overwatering.eyer lemon trees (Citrus x meyeri) are an excellent choice for hobby gardeners. These small trees (6 to 10 feet tall), which thrive in U.S. Department of Agriculture hardiness zones 9 to 11, pack a big punch and bear fruit at an earlier age than other comparable lemon trees.