The Cas9 nuclease and requirements for RNA-guided genome-editing. Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences (Deltcheva et al.2011). A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex (Jinek et al. 2012).he Cas9 nuclease and requirements for RNA-guided genome-editing. Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences (Deltcheva et al. 2011).
Solid Surface Bathroom Countertops: These countertops are made from synthetic materials, so they are manufactured in many colors and styles. The pros of solid surface countertops include the choices, strength and a non-porous surface that cleans up quickly.
Recycled materials are now making their way into kitchen countertop designs. Recycled glass countertops use up to 90% recycled material. Other solid countertop types are made from a high percentage of recycled paper, particle board, wood and metal. There are very attractive options with each recycled and repurposed material that are also very friendly to the environment.