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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
DNase is an enzyme (a protein-like substance) that cuts the DNA present in the mucus. At first DNase was made from cows, but many patients had allergic reactions to it. Then a company separated the gene for human deoxyribonuclease, which chops up the protein but does not cause allergic reactions. DNase is delivered to the patient in aerosol form, and it improves lung function by breaking up the thick mucus in the lungs.