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A mode-locked laser is capable of emitting extremely short pulses on the order of tens of picoseconds down to less than 10 femtoseconds. These pulses will repeat at the round trip time, that is, the time that it takes light to complete one round trip between the mirrors comprising the resonator.
Real-time polymerase chain reaction - Basic principles. 1  Real-time PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore.