An overloading of the gel with a soluble protein creates a semicircular band of this protein (e. g. in the marker lane of the image at 66 KDa), allowing other proteins with similar molecular weights to be covered.
Like arrowroot, tapioca also comes from the root of a plant, however, in this case it is the root of the Cassava plant. It is ground into powdered form and is often referred to as tapioca flour.
The biggest difference between spelt flour and wheat flour is that spelt flour has a harder outer shell on the actual crop. Taking these difference into account will help you understand how to use spelt flour in your cooking.