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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
Confidence votes 21.0K. Ligase here means the reconnection or more specifically the re-splicing together of Dna 'fragments'. Dna ligase's role in Dna replication is to reconnect the Okazaki fragments that are created by the pattern of replication that takes place upon the COMPLEMENTARY 5'-3' strand of DNA.This happens because DNA polymerases can only read the DNA template from the 5' to the 3' end and add free nucleotides in this direction.NA ligase is responsible for joining together fragments of DNA. In DNA replication, after the primers are replaced by DNA Polymerase I, DNA ligase assists in the formation ph â¦ osphodiester bonds between the fragments. This is essential for creating one continuous strand.