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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
Dna ligase. Dna ligase. (Science: enzyme molecular biology) enzyme involved in dna replication. The dna ligase of E. Coli seals nicks in one strand of double stranded dna, a reaction required for linking precursor fragments during discontinuous synthesis on the lagging strand.he dna ligase of E. Coli seals nicks in one strand of double stranded dna, a reaction required for linking precursor fragments during discontinuous synthesis on the lagging strand.