The Cas9 nuclease and requirements for RNA-guided genome-editing. Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences (Deltcheva et al.2011). A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex (Jinek et al. 2012).he Cas9 nuclease and requirements for RNA-guided genome-editing. Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences (Deltcheva et al. 2011).
Solid Surface Bathroom Countertops: These countertops are made from synthetic materials, so they are manufactured in many colors and styles. The pros of solid surface countertops include the choices, strength and a non-porous surface that cleans up quickly.
Quartz countertops, made from crushed quartz and resin binders, is a popular alternative to solid stone counters often found in home settings such as marble or granite. Quartz countertops, being made from a manufactured material, are less expensive than other stone types.emove the countertop from the cabinets and place on a sawhorse for cutting. Spray the tape along the cutting line with water to cool the saw blade. Put on a pair of work gloves and safety goggles to protect from possible quartz chips. Use a circular saw with a diamond blade attached to cut the quartz.