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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds. DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication. DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication. Alex Robert.