Adjustments to the concentration of water and salts within the urine takes place mainly in the distal parts of the tubules and in the collecting tubules, by altering the permeability of the membranes of cells lining the nephron walls. These changes are brought upon to two hormones, ADH and aldosterone.
Use for Colorimetric and Turbidimetric Determinations. These photometric colorimeters use specific light filters to give readings on a unique KlettTM scale. The readings are directly proportional to the concentration or optical density of a solution, in accordance with Beers Law.
After the sample has been applied to the paper and the paper has been equilibrated with the buffer. The current is switched on. Commonly used buffers are, The device providing stable voltage (or) current is available.) Quantitative estimation: The color density of the zone may be multiplied with the area of the zone and the resulting value would be a rough estimate of the concentration of the component. Paper Electrophoresis is one of the zone electrophoresis. This is very important method in all laboratories.