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In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.
DNA Polymerase-The enzyme responsible for catalyzing the addition of nucleotide substrates to DNA both during and after DNA replication. Primase-The enzyme responsible for initiating synthesis of RNA primers on the lagging strand during DNA replication.Holoenzyme-A term used to describe a collection of different enzymes that work together in a given process such as DNA replication.NA Polymerase-The enzyme responsible for catalyzing the addition of nucleotide substrates to DNA both during and after DNA replication. Primase-The enzyme responsible for initiating synthesis of RNA primers on the lagging strand during DNA replication.