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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
How to Clean Surgical Instruments. The Elmasonic S 70 H Ultrasonic Cleaner. Proper surgical instrument cleaning is an exacting process that should be spelled out in standard operating procedures at hospital and healthcare facilities including dental clinics where instruments are reused. Higher temperatures i.e. 60â°C (140â°F) are required to remove dried blood (which is why instruments should be kept wet before cleaning). 2  Cleaning time depends on the load and extent of the contamination. 3  Let experience be your guide. 4  Cavitation ceases at the end of the set time period.