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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
19 DePuy Synthes TFNA â Proximal Femoral Nailing System Surgical Technique 4 Determine nail length(for long nails 260 mmâ480 mm)Instrument 03.037.006 Radiographic RulerMove the image intensiï¬ er to the distal femur, place the proximal end of the radiographic ruler at the skin mark, and take an AP image.4 DePuy Synthes TFNA â Proximal Femoral Nailing System Surgical Technique Alternative techniqueInstruments 03.037.008 Awl B 8/4.7 mm, curved, cannulated or03.037.007 Awl B 8/4.7 mm, straight, cannulatedThe entry point can also be determined by using the awl.