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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Choice of trim for heated abrasive service. Metal seated ball valves are guaranteed for a maximum. service temperature of 572ËF (Trim symbol 5H) and 932ËF. (Trim symbol 6H). For hard graphite seated ball valves, a. maximum service temperature of 932ËF is also guaranteed. (Trim symbol 3H).