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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Cutting operation is optimal at 6500 RPM within a wide temperature range of 100-degrees down to 20-degress Fahrenheit. VCB3EV-AGGRESSIVE CUTTING 12 INCH BLADE WITH CARBIDE TIPPED TEETH FOR EASY CUTS IN FENCE POSTS. Designed specifically for cutting vinyl fence posts and rails. A neutral, no-hook-angle, 100-count carbide tipped tooth configuration and nominal .090â (2.3 mm) blade thickness ensures a consistent smooth cut with less pull into the vinyl material.