The major subject areas include: 1  The history of the franchise and its officers and directors. 2  A complete description of the business to be franchised. 3  All costs and fees that you will be subject to under the agreement. 4  All obligations of either party to the other during the term of the agreement and thereafter.
The information about collection date of the sample is critical and should be included. A precaution should be taken for the measurement of enzyme activity, related to the blood concentration in filter paper, which is not always constant. This concentration depends mainly on the sample's collection procedures [13] .ackground. Mucopolysaccharidosis IVA (MPS IVA), or Morquio Syndrome type A, is an autosomal recessive disease caused by deficiency of the lysosomal enzyme N-acetylgalactosamine-6-sulfatase (GALNS), resulting in excessive lysosomal storage of keratan sulfate in many tissues and organs.
This assay must only be performed by an authorised radioisotope user and safety procedures for 3H-thymidine must be followed in a designated radioactive area. This assay measures IL-2 and Phytohemagglutinin (PHA) induced proliferation of primary chicken thymocytes by incorporation of 3H-thymidine. To ensure proliferation is due to IL-2 and not other cytokines e.g IL-1Ã, pre-incubation of samples with a neutralizing antibody is essential.