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Each of these activities is required during the process of reverse transcription to convert the single-stranded RNA genome into a double DNA copy, which in turn becomes integrated into the host chromosome of the infected cell catalyzed by a second pol gene-encoded enzyme, called integrase.everse Transcriptase. Reverse transcriptase catalyzes the formation of double-stranded deoxyribonucleic acid (DNA) from a single-stranded ribonucleic acid (RNA) genome.
The polymerase chain reaction catalyzed by Taq DNA polymerase has been used for site-specific mutagenesis. The amplification was primed by two oligodeoxyribonucleotides complementary to insulin receptor cDNA.To direct the synthesis of mutant DNA, mismatches were introduced into one of the primers.se of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis. Kadowaki H, Kadowaki T, Wondisford FE, Taylor SI. Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD.