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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Hold pipe and fitting together for 30 seconds to prevent pipe push-out â longer at low temperatures. Wipe off excess.10. Allow 15 minutes for good handling strength and 2 hours cure time at temperatures above 60Â°F before pressure testing up to 180 psi. Longer cure times may be required at temperatures below 60Â°F or with pipe above 3. DO NOT TEST WITH AIR.