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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
This is the most common type of autoclave found in dental offices in the United States. A typical cycle for wrapped instruments includes heat-up and pressurization time, followed by a 15-to-30-minute cycle during which sterilization is taking place (121Â°C at 15 psi). The fine tactile sensitivity needed during dental procedures is not necessary during instrument cleaning and sterilization; therefore, heavy-duty gloves pose no problem in this regard. Additionally, nitrile utility gloves are available in a variety of sizes, allowing a more secure fit.