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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Close Radius Pipe Bending A pipe bend is classified according to the centerline radius (CLR) of the bend as a ratio to the nominal pipe diameter. 4 N.P.S. pipe that is bent on a 6 CLR is classified as a 1 D bend or 1 times the nominal pipe diameter.his allows for the substitution of a pipe bend for a Long Radius 90 and 45 Butt Weld Elbow Fitting. (The centerline radius of a Long Radius 90 or 45 Elbow that is manufactured to ASME/ANSI B16.9 is also 1 times the nominal pipe size. A 4 Long Radius 90 Elbow is 6 center-to-face.).