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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Iron at low temperature. Iron at maximum sole plate temperature of 110 Â°C. Corresponding to the âPolyacryl, polyamide (nylon), acetateâ setting: if necessary, shiny or pressure-sensitive pieces should be ironed with a press cloth or inside out. Caution when using steam irons (as a rule, work without steam).