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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Also, Hamilton point style AS and SGE cone-tip needles are specially designed for this application. When using an autosampler, consult the instrument manual for the preferred point style.In syringes with point style 5, conical with side port, liquid samples can be ï¬ lled and dispensed through the hole in the side.he hand-ï¬ tting process is ï¬ nely controlled to create a liquid-tight seal between the barrel and the plunger. Microliter syringes are for use with liquids and incorporates a stainless steel plunger that is individually ï¬ tted to its matching syringe barrel.