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The presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end ligation is carried out at 12Â°C to 16Â°C to maintain a good balance between annealing of ends and activity of the enzyme.
Consult medical history. Explain procedure and anticipated effect. Dry area with 2x2 gauze for a maximum effectiveness of topical. Apply topical with cotton tip applicator. Wait for anesthetic to take effect for 3-5 minutes. _____________ is achieved by injecting the solution directly into the tissue at the site of the dental procedure.