As substrate is used up, the enzyme's active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between B and C. To measure enzyme activity ideally, the measurements must be made in that portion of the curve where the reaction is zero order.
About CCRT. Canadian Chihuahua Rescue and Transport (CCRT) is a network of volunteers working together to rescue, transport, foster and provide necessary veterinary care for homeless, abandoned, neglected, or abused Chihuahuas. The idea was created by a Canadian who realized the need in 1996.
A computer incident response team (CIRT) is a group that handles events involving computer security breaches. Although most organizations have measures in place to prevent security problems, such events may still occur unexpectedly and must be handled efficiently by CIRT experts, which include team members from specified departments and specialties.