As substrate is used up, the enzyme's active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between B and C. To measure enzyme activity ideally, the measurements must be made in that portion of the curve where the reaction is zero order.
About CCRT. Canadian Chihuahua Rescue and Transport (CCRT) is a network of volunteers working together to rescue, transport, foster and provide necessary veterinary care for homeless, abandoned, neglected, or abused Chihuahuas. The idea was created by a Canadian who realized the need in 1996.
The Industrial Control Systems Cyber Emergency Response Team (ICS-CERT) operates within the National Cybersecurity and Integration Center (NCCIC) , a division of the Department of Homeland Security's Office of Cybersecurity and Communications (DHS CS&C).CCIC/ICS-CERT is a key component of the DHS Strategy for Securing Control Systems. The primary goal of the Strategy is to build a long-term common vision where effective risk management of control systems security can be realized through successful coordination efforts.