Retention on TLC plates is measured in Rf (retention factor), whereas in column chromatography, it is usually expressed in CV (column volumes): R f = (distance traveled by desired compound) / (distance traveled by solvent) CV = 1 / R f
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As the investment is depreciated, the original basis minus accumulated depreciation results in an adjusted basis. For example, a property purchased for $297,000 with acquisition costs of 3,000 would have an original basis of $300,000.