As substrate is used up, the enzyme's active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between B and C. To measure enzyme activity ideally, the measurements must be made in that portion of the curve where the reaction is zero order.
About CCRT. Canadian Chihuahua Rescue and Transport (CCRT) is a network of volunteers working together to rescue, transport, foster and provide necessary veterinary care for homeless, abandoned, neglected, or abused Chihuahuas. The idea was created by a Canadian who realized the need in 1996.
BIRT is a top-level software project within the Eclipse Foundation, an independent not-for-profit consortium of software industry vendors and an open source community. The project's stated goals are to address a wide range of reporting needs within a typical application, ranging from operational or enterprise reporting to multi-dimensional online analytical processing .