A young goat is called a kid. A female kid is called a doeling, and an uncastrated male is called a buckling. Kaylee Askew 0 7 years ago
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research.It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, potassium chloride and potassium phosphate.he simplest way to prepare a PBS solution is to use PBS buffer tablets or pouches. They are formulated to give a ready-to-use PBS solution upon dissolution in a specified quantity of distilled water. They are available in the standard volumes: 100, 200, 500 and 1000 mL, and 10, 25, 50 and 100 L.
Prepare the Phosphate Buffer. Decide on the concentration of the buffer. Most buffers are used at a concentration between 0.1 M and 10 M. If you make up a concentrated buffer solution, you can dilute it as needed. Decide on the pH for your buffer. This pH should be within one pH unit from the pKa of the acid/conjugate base. So, you can prepare a buffer at pH 2 or pH 7, for example, but pH 9 would be pushing it. Use the Henderson-Hasselbach equation to calculate how much acid and base you need. You can simplify the calculation if you make 1 liter of buffer. Select the pKa value that is closest to the pH of your buffer.