As substrate is used up, the enzyme's active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between B and C. To measure enzyme activity ideally, the measurements must be made in that portion of the curve where the reaction is zero order.
About CCRT. Canadian Chihuahua Rescue and Transport (CCRT) is a network of volunteers working together to rescue, transport, foster and provide necessary veterinary care for homeless, abandoned, neglected, or abused Chihuahuas. The idea was created by a Canadian who realized the need in 1996.
CERT (pronounced SUHRT ), officially called the CERT Coordination Center, is the Internet's official emergency team. CERT (which stands for Computer Emergency Readiness Team) was formed by the Defense Advanced Research Projects Agency ( DARPA ) in November 1988 after the Internet was assaulted in the Internet worm incident.