The specific rotation of a substance is defined as the observed angle of optical rotation Î± when linear-. polarized light is passed through a sample cell of 1 decimeter (=100 mm) optical path length at a sample. concentration of 1 gram per 1 deciliter (=100 mL). Specific rotation of a pure material is a physical property of that material at a given wavelength and. temperature and can be looked up in literature.
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