As substrate is used up, the enzyme's active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between B and C. To measure enzyme activity ideally, the measurements must be made in that portion of the curve where the reaction is zero order.
About CCRT. Canadian Chihuahua Rescue and Transport (CCRT) is a network of volunteers working together to rescue, transport, foster and provide necessary veterinary care for homeless, abandoned, neglected, or abused Chihuahuas. The idea was created by a Canadian who realized the need in 1996.
United States Computer Emergency Readiness Team. The United States Computer Emergency Readiness Team (US-CERT) is an organization within the Department of Homeland Securityâs (DHS) National Protection and Programs Directorate (NPPD). Specifically, US-CERT is a branch of the Office of Cybersecurity and Communications' (CS&C) National Cybersecurity and Communications Integration Center (NCCIC).[2]