As substrate is used up, the enzyme's active sites are no longer saturated, substrate concentration becomes rate limiting, and the reaction becomes first order between B and C. To measure enzyme activity ideally, the measurements must be made in that portion of the curve where the reaction is zero order.
About CCRT. Canadian Chihuahua Rescue and Transport (CCRT) is a network of volunteers working together to rescue, transport, foster and provide necessary veterinary care for homeless, abandoned, neglected, or abused Chihuahuas. The idea was created by a Canadian who realized the need in 1996.
The CLTS approach. What is CLTS? Community Led Total Sanitation (CLTS) is an innovative methodology for mobilising communities to completely eliminate open defecation (OD). Communities are facilitated to conduct their own appraisal and analysis of open defecation (OD) and take their own action to become ODF (open defecation free).