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Western blotting and enzyme-linked immunosorbent assay (ELISA) are widely used in the protein detection (Savige, 1998). The name of Western blotting, it is also called Western blot. ELISA is the abbreviation of enzyme-linked immunosorbent assay (Ma and Shieh, 2006).
Like the ELISA procedure, the western blot is an antibody detection test. However, unlike the ELISA method, the viral proteins are separated first and immobilized. In subsequent steps, the binding of serum antibodies to specific HIV proteins is visualized.ike the ELISA procedure, the western blot is an antibody detection test. However, unlike the ELISA method, the viral proteins are separated first and immobilized. In subsequent steps, the binding of serum antibodies to specific HIV proteins is visualized.