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To determine âcessation of all functions of the entire brain, including the brain stem,â physicians must determine the presence of unresponsive coma, the absence of brainstem reflexes, and the absence of respiratory drive after a CO 2 challenge.
Quick Answer. According to TransWeb, people typically develop serious and possibly irreversible brain damage after 5 to 10 minutes of not breathing. Brain cells begin to die after 1 minute without oxygen. Serious brain damage is likely within 3 minutes without breathing.