1 A cubic yard of driveway-grade crushed gravel costs about $10 -$60 or more, depending on whether it is picked up by the customer at the gravel yard or delivered to the job site, and the size and type of gravel used.( 2 A cubic yard of gravel is 3'x3'x3' and weighs roughly 1.2 to 1.7 tons or more). (A cubic yard of gravel is 3'x3'x3' and weighs roughly 1.2 to 1.7 tons or more). 2  A 12'x25' single-car driveway 9 thick requires an estimated 10-14 tons, which can cost $100 -$850.
HMG-CoA reductase is anchored in the membrane of the endoplasmic reticulum, and was long regarded as having seven transmembrane domains, with the active site located in a long carboxyl terminal domain in the cytosol. More recent evidence shows it to contain eight transmembrane domains.
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon.It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening. Applications. â¢ Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies.t inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.