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This unzipping takes place in both directions from the replication origins, creating a replication bubble. The replication is therefore said to be bi-directional. Once the two strands are separated a small piece of RNA, called an RNA primer, is attached to the DNA by an enzyme called DNA primase. These primers are the beginnings of all new DNA chains since the enzyme responsible for the copying of the DNA, DNA polymerase, can not start from scratch.
The newly made lagging strand still is synthesized 5'-3'. However, since the DNA is oriented in a manner that does not allow continual synthesis, only small sections can be read at a time. An RNA primer is placed on the DNA strand 3' to the origin of replication.Just as before, DNA Polymerase reads 3'-5' on the original DNA to produce a 5'-3' nascent strand. Polymerase reaches the origin of replication and stops replication until a new RNA primer is placed 3' to the last RNA primer.he newly made lagging strand still is synthesized 5'-3'. However, since the DNA is oriented in a manner that does not allow continual synthesis, only small sections can be read at a time. An RNA primer is placed on the DNA strand 3' to the origin of replication.