The LSRPA is a non-profit professional membership organization established to provide information, education and technical resources to LSRPs, other professionals involved in environmental remediation in the State of New Jersey and students pursuing an education in a discipline related to environmental remediation.
Western blotting and enzyme-linked immunosorbent assay (ELISA) are widely used in the protein detection (Savige, 1998). The name of Western blotting, it is also called Western blot. ELISA is the abbreviation of enzyme-linked immunosorbent assay (Ma and Shieh, 2006).
WESTERN BLOTTING TIPS AND TROUBLESHOOTING GUIDE TIPS FOR SUCCESSFUL WESTERB BLOTS TROUBLESHOOTING GUIDE 1. Suboptimal protein transfer. This is the most common complaint with western blotting and could be due to many different problems listed below. a) Low quality membrane used for transfer. High quality PVDF membrane is the blotting membrane of choice as low molecular weight proteins are sometimes transferred through nitrocellulose membranes and therefore may be not visible on the blot. b) Improper Western transfer procedure. c) For semi-dry Western transfers make sure the filter paper is wet.