The LSRPA is a non-profit professional membership organization established to provide information, education and technical resources to LSRPs, other professionals involved in environmental remediation in the State of New Jersey and students pursuing an education in a discipline related to environmental remediation.
Western blotting and enzyme-linked immunosorbent assay (ELISA) are widely used in the protein detection (Savige, 1998). The name of Western blotting, it is also called Western blot. ELISA is the abbreviation of enzyme-linked immunosorbent assay (Ma and Shieh, 2006).
Oh ya! But if you donât quantify your Western blots correctly youâll find yourself in an unpleasant, unrepeatable and totally meaningless place. And while some scientists are okay dwelling in a meaningless place, I hope you are not. Review these important concepts about how to correctly quantitate your next Western blot. 1. Find the Linear Range. For quantitate analysis of an image you must ensure your image was captured in a manner sensitive enough to detect change, in what we call the âlinear rangeâ.