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Protein in the Roast Beef Sandwich. A 6-inch Subway roast beef sandwich contains 24 grams of protein. That's 52 percent of the 46 grams of protein that women need each day, according to the Centers for Disease Control and Prevention. It's 43 percent of the 56 grams of protein men should aim for each day.
Portion size is also important, as too large a sandwich could provide you with more calories than you need. Half of a sandwich made from one slice of whole wheat bread and 2 ounces of turkey with mustard contains about 200 calories. Your sandwich will be healthier if you add some vegetables, and more filling as well.