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To determine âcessation of all functions of the entire brain, including the brain stem,â physicians must determine the presence of unresponsive coma, the absence of brainstem reflexes, and the absence of respiratory drive after a CO 2 challenge.
In addition, there should be evidence that the patient has experienced a disease or injury that could cause brain death. A final determination of brain death must involve demonstration of the total lack of electrical activity in the brain by two electroencephalographs (EEGs) taken twelve to twenty-four hours apart.