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Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. It is the polymerase used by a bacteria, Thermus auquatics, which... Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.
The standard PCR enzymes are not surprisingly quite accurate. Even Thermus aquaticus (Taq) DNA polymerase, a low fidelity PCR polymerase, only makes a mistake once in approximately 100,000 nucleotide insertions, depending on reaction conditions (4).The basis of polymerase accuracy is an exciting area of investigation.uring PCR, Taq DNA Polymerase will misincorporate nucleotides at a particular rate leading to mismatch formation. This is termed âerror rateâ. Taq, and polymerases in general, will stall at these mismatched bases and are more likely to dissociate before extending as compared to correctly base paired 3Â´ ends.