ENGINEERING.com has updated it's forum. To post a question please visit the new Ask@ Forum. With a database of over 10000 questions the library will remain available for an extended period.
Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. It is the polymerase used by a bacteria, Thermus auquatics, which... Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.
Taq makes DNA products that have A (adenine) overhangs at their 3' ends. This may be useful in TA cloning, whereby a cloning vector (such as a plasmid) that has a T (thymine) 3' overhang is used, which complements with the A overhang of the PCR product, thus enabling ligation of the PCR product into the plasmid vector.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity. Originally its error rate was measured at about 1 in 9,000 nucleotides.