Talk with your caregiver about the best time to wean your baby. 1  Introducing solid foods: Weaning should begin when your baby is about 6 months old.  Weaning from breast milk: Your baby needs nutrients from breast milk until he is at least 12 months old.
Enzyme-linked secondary antibodies are applied as detection antibodies that also bind specifically to the antibody's Fc region (nonspecific). The plate is washed to remove the unbound antibody-enzyme conjugates. A chemical is added to be converted by the enzyme into a color or fluorescent or electrochemical signal.
After the remaining binding sites are blocked with a detergent or protein reagent, the blot is probed with the primary antibody and then washed. The antibody-antigen complexes are subsequently identified by horseradish peroxidase (HRP) or alkaline phosphatase (AP) coupled to the secondary anti-IgG antibody.