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Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. It is the polymerase used by a bacteria, Thermus auquatics, which... Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.
Taq' s optimum temperature for activity is 75â80Â°C, with a half-life of greater than 2 hours at 92.5Â°C, 40 minutes at 95Â°C and 9 minutes at 97.5Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72Â°C.ne of Taq' s drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in relatively low replication fidelity.