Talk with your caregiver about the best time to wean your baby. 1  Introducing solid foods: Weaning should begin when your baby is about 6 months old.  Weaning from breast milk: Your baby needs nutrients from breast milk until he is at least 12 months old.
Enzyme-linked secondary antibodies are applied as detection antibodies that also bind specifically to the antibody's Fc region (nonspecific). The plate is washed to remove the unbound antibody-enzyme conjugates. A chemical is added to be converted by the enzyme into a color or fluorescent or electrochemical signal.
1 A specific antibody is added, and binds to antigen (hence the 'sandwich': the Ag is stuck between two antibodies); 2  Apply enzyme-linked secondary antibodies as detection antibodies that also bind specifically to the antibody's Fc region (non-specific).andwich ELISA Protocol is shown in a different section. Sandwich ELISA is a common tool to diagnose Influenza, e.g. H5N1 (Avian Flu) Hemagglutinin ELISA kit. In addition, a description of the application of sandwich ELISA to home pregnancy test can be found here.