The reason for this is that the genomic RNA in cells with an active replicon is a mixture of molecules. One fraction is synthesized from the runoff MG by T7 RNA polymerase (input RNA), and the other fraction results from the replication of the antigenomic RNA by Lassa virus polymerase.n inhibitory effect of Z protein was previously demonstrated with the LCMV and Tacaribe virus replicon systems (12, 32). We therefore wondered whether a similar effect is also observed in the Lassa virus replicon system. Various amounts of pCITE-Z were cotransfected with pCITE-NP, pCITE-L, and genomic MG DNA or RNA.
The North Carolina Minimum Wage is the lowest hourly wage that an employee working in North Carolina can legally be paid. Both North Carolina and the federal government have set a legal minimum wage - North Carolina's is $7.25 per hour, while the federal minimum wage is $7.25 per hour.
The current minimum wage in West Virginia is $8.75 per hour. West Virginia raised their minimum wage above the $7.25 federal minimum wage for the first time on December 31, 2014. The state minimum wage was raised to $8.75 on December 31, 2015. West Virginia's minimum wage is applicable to all employers of 6 or more employees, unless they are specifically exempt.