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Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. It is the polymerase used by a bacteria, Thermus auquatics, which... Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.
In the presence of Mn 2+ , Tfl DNA polymerase can use RNA as a template. Tfl DNA polymerase exhibits a 5â²â3â² exonuclease activity but lacks a 3â²â5â² exonuclease activity. This enzyme is commonly used in PCR (Gaensslen et al. 1992), where its activity is similar to that of Taq DNA polymerase.Tfl DNA polymerase is used in the Access and AccessQuickâ¢ RT-PCR Systems.aq DNA polymerase is suitable for most PCR applications that do not require a high-fidelity enzyme, such as detecting specific DNA or RNA sequences. The error rate of Taq DNA polymerase is approximately 1 Ã 10 â5 errors/base, although the fidelity does depend somewhat on the reaction conditions.