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From Wikipedia, the free encyclopedia. A true breeding organism, sometimes also called a purebred, is an organism that always passes down certain phenotypic traits (i.e. physically expressed traits) to its offspring.
Mendel then crossed a true-breeding purple flower plant and a true-breeding white flower plant. This is called a monohybrid experiment. A test cross is performed between two plants that breed true for one trait, and the resulting trait for each offspring plant is determined. The first, true-breeding generation, is called the parent, P generation.