An association football (soccer) match is presided over by a referee, whom the Laws of the Game give full authority to enforce the Laws of the Game in connection with the match to which he has been appointed (Law 5). The referee is oftentimes assisted by two assistant referees, and sometimes by a fourth official.
Barfoed's test. Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu2O), which forms a brick-red precipitate.
The ultimate purpose of the biuret test is to determine the concentration of protein in solutions. In order to do that, you must first construct from your data a standard curve, which provides a reference, or baseline, that you can compare unknown solutions against.