The reason for this is that the genomic RNA in cells with an active replicon is a mixture of molecules. One fraction is synthesized from the runoff MG by T7 RNA polymerase (input RNA), and the other fraction results from the replication of the antigenomic RNA by Lassa virus polymerase.n inhibitory effect of Z protein was previously demonstrated with the LCMV and Tacaribe virus replicon systems (12, 32). We therefore wondered whether a similar effect is also observed in the Lassa virus replicon system. Various amounts of pCITE-Z were cotransfected with pCITE-NP, pCITE-L, and genomic MG DNA or RNA.
The North Carolina Minimum Wage is the lowest hourly wage that an employee working in North Carolina can legally be paid. Both North Carolina and the federal government have set a legal minimum wage - North Carolina's is $7.25 per hour, while the federal minimum wage is $7.25 per hour.
The West Virginia Minimum Wage is greater than the Federal Minimum Wage of $7.25 per hour, so employees in West Virginia are entitled to the higher minimum wage of $8.00 per hour. Please Note: Twenty states have raised their minimum wage as of January 1, 2015.