Talk with your caregiver about the best time to wean your baby. 1  Introducing solid foods: Weaning should begin when your baby is about 6 months old.  Weaning from breast milk: Your baby needs nutrients from breast milk until he is at least 12 months old.
Enzyme-linked secondary antibodies are applied as detection antibodies that also bind specifically to the antibody's Fc region (nonspecific). The plate is washed to remove the unbound antibody-enzyme conjugates. A chemical is added to be converted by the enzyme into a color or fluorescent or electrochemical signal.
Introduction. Sandwich ELISA (enzyme-linked immunosorbent assay) involves attachment of a capture antibody to a microplate. Then, samples containing unknown amount of the target protein or analyte of interest are added and bind to the capture antibody.he ELISA method is a benchmark for quantitation of antigens. ELISAs are adaptable to high-throughput screening because results are rapid, consistent and relatively easy to analyze. The best results have been obtained with the sandwich format, utilizing highly purified, pre-matched capture and detection antibodies.