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From Wikipedia, the free encyclopedia. A true breeding organism, sometimes also called a purebred, is an organism that always passes down certain phenotypic traits (i.e. physically expressed traits) to its offspring.
That is, they bred true. Also, 1/4 of the F2 (2nd generation) plants in Mendel's experiments bred true as tall plants and 1/4 bred true as short plants. The same pattern occurred for the other traits Mendel measured. For example, parent plants produced only smooth (S) or wrinkled (s) seeds when self-pollinated.