Talk with your caregiver about the best time to wean your baby. 1  Introducing solid foods: Weaning should begin when your baby is about 6 months old.  Weaning from breast milk: Your baby needs nutrients from breast milk until he is at least 12 months old.
Enzyme-linked secondary antibodies are applied as detection antibodies that also bind specifically to the antibody's Fc region (nonspecific). The plate is washed to remove the unbound antibody-enzyme conjugates. A chemical is added to be converted by the enzyme into a color or fluorescent or electrochemical signal.
Overview of ELISA. ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones.Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.n an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product.