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Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. It is the polymerase used by a bacteria, Thermus auquatics, which... Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.
Aliquot 22.75 Âµl of the PCR Master Mix without Taq Polymerase to the No EnzymeControls.10. Add Taq Polymerase (0.25 Âµl x number of remaining reactions) to the PCR âMasterMixâ and mix. Aliquot 23Âµl of the mixture into tubes containing experimental samplesand Control Template dilutions.