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Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. It is the polymerase used by a bacteria, Thermus auquatics, which... Taq polymerase is an enzyme that is used by scientists in the polymerase chain reaction because it is stable at high temperatures. Large amounts of a sample of DNA are necessary for molecular and genetic analyses, and the polymerase chain reaction is used to amplify very small amounts of DNA.
The polymerase chain reaction catalyzed by Taq DNA polymerase has been used for site-specific mutagenesis. The amplification was primed by two oligodeoxyribonucleotides complementary to insulin receptor cDNA.To direct the synthesis of mutant DNA, mismatches were introduced into one of the primers.se of polymerase chain reaction catalyzed by Taq DNA polymerase for site-specific mutagenesis. Kadowaki H, Kadowaki T, Wondisford FE, Taylor SI. Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD.