It is for this reason that is referred to as a complementary DNA or cDNA. Though there are numerous processes for synthesizing cDNA, the best way of doing so is by using mature or fully spliced mRNA. This is usually done by using the enzyme reverse transcriptase.
Ammonia is used as refrigerant prominently in the refrigeration systems of food industry like dairies, ice creams plants, frozen food production plants, cold storage warehouses, processors of fish, poultry and meat and number of other applications.
Free ammonia is a chemical compound of nitrogen and hydrogen with the formula NH3. Technically ammonia in water is called Ammonium hydroxide. In the aquarium hobby the term ammonia also refers to an ionized form called ammonium (NH4+).These are held in equilibrium in the water [Citation needed]. ' Total ammonia ' (TA) or 'Total ammonia nitrogen' (TAN) refers to the total concentration of both NH3 and NH4+ in the aquarium water.Where you have Ammonia, you will also have Ammonium. The ratio of one to the other varies on pH and water temperature.So it is important to know these two readings when measuring Ammonia in your tank to gauge how serious the Ammonia levels are. See the Links section for a Toxic Ammonia calculator. Here is a table showing the varying levels of ammonia against ammonium.he ratio of one to the other varies on pH and water temperature. So it is important to know these two readings when measuring Ammonia in your tank to gauge how serious the Ammonia levels are. See the Links section for a Toxic Ammonia calculator. Here is a table showing the varying levels of ammonia against ammonium.