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Bacterial neutral proteases are active in a narrow pH range (pH 5 to 8) and have relatively low thermotolerance. Due to their intermediate rate of reaction, neutral proteases generate less bitterness in hydrolyzed food proteins than do the animal proteinases and hence are valuable for use in the food industry. Neutrase, a neutral protease, is insensitive to the natural plant proteinase inhibitors and is therefore useful in the brewing industry. The bacterial neutral proteases are characterized by their high affinity for hydrophobic amino acid pairs.
MacConkey Agar. This is an agar upon which only Gram-negative bacteria can grow. What is more is that E.coli will grow into red colonies, as there is a pH indicator present. It should be mentioned that MacConkey agar powder comes in two versions: one with the sugar lactose in it, and one without any added sugars.ne might ask why agar, as opposed to regular gelatin (like that found in Jello), is used for culturing bacteria. The answer is agar, unlike gelatin, won't be degraded (eaten) by bacteria. Also, agar is firmer and stronger than gelatin.