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The cooling is thought to work by slowing down the operation of enzymes involved in sex determination. The cooling has to be done very precisely. Chickens try to keep their eggs at a steady 37.5C below their body. This is the temperature used in commercial incubators. drop in temperature can change the sex of chickens. A brief, chilly spell can change the sex of chickens in the egg, the festival learnt yesterday.
Although not absolutely proven, it seems the female embryo does not survive the extra temperature during development. Note that the hatch will be reduced by the number of potential hen chicks that do not survive. Optimum temperature for best hatch, male and female is 100.5 degrees F. with a 60%+ humidity.1 person found this useful.etired in 2005, breeding Araucana chickens. Some studies have shown that a slight increase in incubator temperature over the full 21 day incubation period will result in a higher male to female ratio.