It is for this reason that is referred to as a complementary DNA or cDNA. Though there are numerous processes for synthesizing cDNA, the best way of doing so is by using mature or fully spliced mRNA. This is usually done by using the enzyme reverse transcriptase.
Ammonia is used as refrigerant prominently in the refrigeration systems of food industry like dairies, ice creams plants, frozen food production plants, cold storage warehouses, processors of fish, poultry and meat and number of other applications.
HFO Refrigerants. HFO (hydrofluoro-olefin) refrigerants are the fourth generation of fluorine-based refrigerants. HFC refrigerants are composed of hydrogen, fluorine and carbon atoms connected by single bonds between the atoms.