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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
The final and third. step is applying a counter stain. A counter stain is used to stain all unstained biological. materials. Counter stains are usually a contrasting color to the primary stain. With the. Gram stain, the primary stain colors the cells violet and the counter stained bacteria are. red. Violet cells are âGram positiveâ because they have reacted to the stain.