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A basic stain stains the bacteria (the stain is a positively charged molecule that attaches to cells which are negatively charged or to negatively charged molecules in cells.) A negative stain does not stain cells (why its called a negative stain), it stains the background around the cells.
Gram-Negative. Gram-negative bacteria do not retain the crystal violet stain because of its physical makeup and will stain pink. Thus, why they are called gram-negative. You also need a microscope to see this stain coloring. The microbes are still very tiny and are usually placed on a slide prior to staining.ram-negative bacteria do not retain the crystal violet stain because of its physical makeup and will stain pink. Thus, why they are called gram-negative. You also need a microscope to see this stain coloring. The microbes are still very tiny and are usually placed on a slide prior to staining.