It is for this reason that is referred to as a complementary DNA or cDNA. Though there are numerous processes for synthesizing cDNA, the best way of doing so is by using mature or fully spliced mRNA. This is usually done by using the enzyme reverse transcriptase.
Ammonia is used as refrigerant prominently in the refrigeration systems of food industry like dairies, ice creams plants, frozen food production plants, cold storage warehouses, processors of fish, poultry and meat and number of other applications.
In some cases, 100 times that of CO2. These gases are controlled by legislation known as F Gas regulations thus placing strict limits on the use of these gases and the necessity to detect them. Ammonia has been used for over a century as refrigeration gas and has no global warming potential or effect on the ozone level.The drawback of Ammonia is that it is very toxic with a pungent odour and, in high concentrations, can be flammable. However, if the Ammonia can reach the food areas, low level of Ammonia detection is essential to prevent the large financial losses that can ensue from a leak no matter how small. 2  The plant room is always subject to small leaks due to moving equipment, valves and joints, so a background of Ammonia can be expected.