Wal Mart de Mexico SAB de CV News. Mexico's Walmex says same-store sales rise 4.7 percent in March. MEXICO CITY Mexico's biggest retailer, Wal-Mart de Mexico, said on Thursday sales at its Mexican stores that have been open at least a year rose 4.7 percent in March compared to the same month last year.
There are several steps that need to be carried out when isolating mitochondrial DNA from tissue sample: 1) Tissue homogenization. Tissue homogenization represents the first step in the mitochondrial DNA (mtDNA) isolation workflow when starting with tissues e.g., muscle, heart, liver, brain, or kidney. To obtain sufficient amount of mtDNA for downstream analysis sufficient amount of mitochondria need to be prepared.
Isolation of plasmid DNA and analysis of isolated plasmid. Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words, this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells.You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a).To this end you have (1) isolated total chromosomal DNA from your assigned bacterial strain and digested this DNA with the restriction enzyme BamHI.o this end you will digest 10 uL of the isolated plasmid with restriction enzyme BamHI. The 40 uL of the plasmid DNA will be quantified using Nanodrop spectro-photometry. A predetermined amount of the DNA will be submitted for sequence analysis.