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Since the first therapeutic application of a r-protein produced in mammalian cells was approved in 1986. (human tissue plasminogen activator, tPA, Genentech), biopharmaceuticals have changed the. landscape for the treatment of many diseases, including many types of cancer, rheumatic conditions. and orphan diseases.
Production of ROS was dependent on the concentration of juglone; the highest concentration of juglone (250 Î¼M) caused the highest overproduction of ROS. Overproduction of ROS was closely connected with the changes in mitochondria and endoplasmic reticulum.