Brigitta gives Dean a second, stronger dose of poison through her touch and tells him, That's for our father, implying that the djinn Sam and Dean killed in 2.20 was her father, and the other two djinn with her are her brothers.
COMMON MECHANISMS 47 adduct is formed, which has been used as a biomarker of effect (Albrecht and Neumann, 1985, Neumann, 2000, Sarkar et al. (2006). The use of Hb-adducts as biomarkers clearly demonstrates that the general population is exposed to many monocyclic and polycyclic aromatic amines (Bryant et al.owever, the models help to verify the proposed mode of action and in fact support it. In summary, a common mode of action is at the basis of the carcinogenic properties of aromatic amines and a carcinogenic potential seems to be associated with this whole group of chemicals.
Bromination on aromatic ring: toluene has one methyl group on aromatic group on benzene ring which imparts positive inductive effect and hyperconjugation. Because of these two effects, methyl groups increases the electron density on aromatic ring and acts as activating group.he methyl group on benzene ring in toluene is ortho and para directing and activating group due to hypercojugation and positive inductive effect of methyl group. Out of ortho and para products, ortho products are formed as major products around 63%, with para 34% and 3% meta products.