Gel Analysis of Bisulfite Converted DNA. When using an agarose gel to analyze the converted DNA, use a 2% gel with a 100 bp marker. When using gels it can be necessary to load up to 100 ng of sample to visualize the DNA. Many researchers worry when at first they canât see any band in their gel.
Converting PST to EST. This time zone converter lets you visually and very quickly convert PST to EST and vice-versa. Simply mouse over the colored hour-tiles and glance at the hours selected by the column... and done! PST stands for Pacific Standard Time. EST is known as Eastern Standard Time. EST is 3 hours ahead of PST.
Central time or CST is two-hours ahead of Pacific time or PST. Therefore, if it is 7am PST, it is 9am CST. For reference, the east coast is three hours ahead of the west coast and the east coast is one-hour ahead of central time.