1 This will occur only if the egg has been united with a male sperm cell (fertilized). 2  A chick will hatch from a fertile egg if the egg is incubated at 100.5 degrees F for 21 days. 3  You can tell if an egg is fertile by candling and identifying a mass or small spot in the egg.
I. TYPES OF CELLS GROWN IN CULTURE. Tissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often used interchangeably. Cell cultures are derived from either primary tissue explants or cell suspensions.Primary cell cultures typically will have a finite life span in culture whereas continuous cell lines are, by definition, abnormal and are often transformed cell lines.ell cultures are derived from either primary tissue explants or cell suspensions. Primary cell cultures typically will have a finite life span in culture whereas continuous cell lines are, by definition, abnormal and are often transformed cell lines.
5.1 Primary Cultures. Primary cultures are derived directly from excised, normal animal tissue and cultures either as an explant culture or following dissociation into a single cell suspension by enzyme digestion. Such cultures are initially heterogeneous but later become dominated by fi broblasts..3 Culture Morphology. In terms of growth mode cell cultures take one of two forms, growing either in suspension (as single cells or small free fl oating clumps) or as a monolayer that is attached to the tissue culture fl ask.