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The main enzymes involved in DNA replication are: Helicase: unwinds the double helix into two strands Polymerase: adds nucleotides to an existing strand Ligase: brings togeth â¦ er the Okazaki fragments Topoisomerase: cuts and rejoins the helix RNA primase: catalyzes the synthesis of RNA primers Hope this helps!
DNA Replication. INTRODUCTION. DNA replication is the process whereby an entire double-stranded DNA is copied to produce a second, identical DNA double helix. To understand the functions of the proteins responsible for DNA replication, including helicase, SSB protein, primase, the sliding clamp, DNA polymerase, Rnase H and DNA ligase. to understand why the leading strand is synthesized continuously and the lagging strand is synthesized discontinuously.