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Types of Base Pairs. Each nitrogenous base has a partner. In DNA, adenine and thymine pair up, and so do guanine and cytosine. In the related molecule RNA, thymine is replaced by its close relative uracil, so the pairs are adenine-uracil and guanine-cytosine.
The nitrogenous bases point inward on the ladder and form pairs with bases on the other side, like rungs. Each base pair is formed from two complementary nucleotides (purine with pyrimidine) bound together by hydrogen bonds. The base pairs in DNA are adenine with thymine and cytosine with guanine. DNA has a spiral staircase-like structure. The steps are formed by the nitrogen bases of the nucleotides where adenine pairs with thymine and cytosine with guanine.