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The main enzymes involved in DNA replication are: Helicase: unwinds the double helix into two strands Polymerase: adds nucleotides to an existing strand Ligase: brings togeth â¦ er the Okazaki fragments Topoisomerase: cuts and rejoins the helix RNA primase: catalyzes the synthesis of RNA primers Hope this helps!
DNA Polymerase III is responsible for adding new nucleotides to the strand being synthesised. Also involved in DNA replication are DNA Polymerase I which replaces primers wi â¦ th nucleotides, and DNA Ligase which joins fragments of DNA together.1 person found this useful.NA Polymerase III is responsible for adding new nucleotides to the strand being synthesised. Also involved in DNA replication are DNA Polymerase I which replaces primers wi â¦ th nucleotides, and DNA Ligase which joins fragments of DNA together. 1 person found this useful.