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Taking a puppy away from its mother in the transitional stage (2-4 weeks) can create a number of problems for later life. Any time before 8 weeks even, can cause problems â so that a puppy is pre-disposed to become fearful of other dogs. Ideally the whole litter should be kept together until at least 8 weeks.
If she has milk,then it shouldn't be long.Call your vet just in case there is a problem,and take her and the pups t the vet within 48 hours after the birth to get the mom a shot of pitocin,which will help to expel any retained placentas,puppies and other gunk, and to make sure that the pups are all healthy.