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The main enzymes involved in DNA replication are: Helicase: unwinds the double helix into two strands Polymerase: adds nucleotides to an existing strand Ligase: brings togeth â¦ er the Okazaki fragments Topoisomerase: cuts and rejoins the helix RNA primase: catalyzes the synthesis of RNA primers Hope this helps!
DNA polymerase III (not DNA polymerase) is an enzyme that works in association with other enzymes during the replication of a DNA molecule. DNA replication begins when the enz â¦ yme, known as helicase unwinds a DNA strand.Helicase unwinds a DNA strand, thus, in the process, separating the two DNA templates. polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand.   DNA polymerase differs from RNA polymerase in two major respects:   * â¦ Like all enzymes, DNA polymerase is substrate-specific.