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The main enzymes involved in DNA replication are: Helicase: unwinds the double helix into two strands Polymerase: adds nucleotides to an existing strand Ligase: brings togeth â¦ er the Okazaki fragments Topoisomerase: cuts and rejoins the helix RNA primase: catalyzes the synthesis of RNA primers Hope this helps!
(It is the 3Â´ -OH group thatis required to create the next phosphodiester bond.) The RNA is subsequentlyremoved and replaced with DNA so that no gaps exist in the final product. 4. Helicases are enzymes that disrupt the hydrogen bonds that hold the two DNAstrands together in a double helix.This breakage is required for both RNA andDNA synthesis. Topoisomerases are enzymes that create and relax supercoiling inthe DNA double helix.It is the 3Â´ -OH group thatis required to create the next phosphodiester bond.) The RNA is subsequentlyremoved and replaced with DNA so that no gaps exist in the final product. 4. Helicases are enzymes that disrupt the hydrogen bonds that hold the two DNAstrands together in a double helix.