-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Melania Trump is the current First Lady of the United States and wife of the 45th U.S. President Donald Trump. She was born in Novo Mesto, Slovenia and grew up in Sevnica, in the Lower Sava Valley. She worked as a fashion model for agencies in Milan and Paris, later moving to New York City in 1996. Her modeling career was associated with Irene Marie Models and Trump Model Management. In 2001, she became a permanent resident of the United States. She married Donald Trump in 2005 and obtained U.S. citizenship in 2006. She is the first naturalized U.S. citizen to become First Lady of the United States.