1 Breathe in deeply through your nose, expanding your abdomen and then filling your lungs with air. 2  Hold your breath for a couple of seconds. 3  Breathe out slowly through your mouth and empty your lungs completely.  Repeat for 5 to 10 minutes.
PCR is the enzymatic amplification of a specific DNA sequence in vitro9. This process uses multiple cycles of template denaturation, primer annealing, and primer elongation to amplify DNA sequences. It is an exponential process since the amplified products from each previous cycle serve as templates for the next cycle of amplification,
Molecular cloning refers to the process of making multiple molecules. Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA.loning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms.