-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
They dated for a few years and attended events, like the 2002 NBA Finals. Trump, who had been married twice previously, finally popped the question in 2004. Melania's ring is huge. On January 22, 2005, Donald and Melania tied the knot at Trump's Mar-A-Lago estate in Palm Beach, Florida. Melania wore a $200,000 gown by Christian Dior. The couple welcomed their son, Barron William Trump, on March 20, 2006. Melania stood by Donald as he received his star on the Hollywood Walk of Fame in 2007.