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Semen is made up of sperm (male cells for sexual reproduction) floating in a fluid called seminal plasma. Sperm, also called spermatozoa, are made by a man's testicles and mature (grow up) in the epididymis. The fluids in seminal plasma come from different glands in the man's body: the seminal vesicles, prostate and bulbourethral glands (also called the Cowper's glands).
Testosterone is made primarily by the testicles in males. Small amounts are also made by the adrenal glands and the ovaries in females. In males it is the main sex hormone and plays an important role in the development of the male reproductive system.