IHC: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:150 with AutoProbe III (GTX28620). Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.
Symmetry arguments suggest that most ligands engage metals via multiple bonds. The term 'metal ligand multiple bond is often reserved for ligands of the type CR n and NR n (n = 0, 1, 2) and OR n (n = 0, 1) where R is H or an organic substituent, or pseudohalide.s a cautionary note, the classification of a metal ligand bond as being multiple bond order is ambiguous and even arbitrary because bond order is a formalism.
The atom within a ligand that is bonded to the central atom or ion is called the donor atom. In a typical complex, a metal ion is bound to several donor atoms, which can be the same or different. Polydentate (multiple bonded) ligands consist of several donor atoms, several of which are bound to the central atom or ion.uperficially lanthanide complexes are similar to those of the transition metals in that some are coloured. However for the common Ln 3+ ions (Ln = lanthanide) the colors are all pale, and hardly influenced by the nature of the ligand.