-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Born on April 26, 1970, First Lady Melania Trump is a former model and the third wife of U.S. President, real estate billionaire and former reality TV star Donald Trump. Early Life Melania Trump (born Melanija Knavs, Germanized to Melania Knauss) was born on April 26, 1970, in Novo Mesto, Slovenia (then part of communist Yugoslavia).