-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Melania Trump modeled for a number of publications, including FHM magazine in 2000. During the early part of her modeling career, she also changed her name to the more German-sounding Knauss. Melania came to New York in 1996, moved into an apartment by Union Square in Lower Manhattan and made her own way.