-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Born on April 26, 1970, Melania is 46 - meaning there is a 24 year age gap to her 70-year-old husband. She was raised by her Communist party member father Viktor Knavs, who managed car dealerships for a state-owned vehicle manufacturer, and her mother Amalija Ulnik, a pattern maker at a textile factory.