-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Such was especially true for Donald and Melania's January 2005 nuptials at Trump's resort in Palm Beach, Florida. For the wedding, Melania wore agown made by John Galliano, which reportedly took 1,000 hours to make, 550 hours of which were dedicated to hand-sewing 1,500 crystal rhinestones and pearls.