-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Melania Knauss-Trump life and biography. Melania Knauss-Trump biography. Melania Knauss-Trump, born Melanija Knavs, germanized to Melania Knauss is a Slovenian model and an American socialite. She is married to American real estate magnate Donald Trump. Better known these days as Donald Trump's wife, 5' 11 Slovenian model Melania Knauss grew up in a household where constant studying was the rule.