1 Breathe in deeply through your nose, expanding your abdomen and then filling your lungs with air. 2  Hold your breath for a couple of seconds. 3  Breathe out slowly through your mouth and empty your lungs completely.  Repeat for 5 to 10 minutes.
PCR is the enzymatic amplification of a specific DNA sequence in vitro9. This process uses multiple cycles of template denaturation, primer annealing, and primer elongation to amplify DNA sequences. It is an exponential process since the amplified products from each previous cycle serve as templates for the next cycle of amplification,
.At a lower temperature, each strand is then used as the template in DNA synthesis by the DNA polymerase to selectively amplify the target DNA. The selectivity of PCR results from the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions. ^ Thus, the region of DNA to be synthesized is defined by the primers, which specifically anneal themselves to their complementary sequences on template strand, limiting the DNA fragment that will be amplified 9,46 .