1 Breathe in deeply through your nose, expanding your abdomen and then filling your lungs with air. 2  Hold your breath for a couple of seconds. 3  Breathe out slowly through your mouth and empty your lungs completely.  Repeat for 5 to 10 minutes.
PCR is the enzymatic amplification of a specific DNA sequence in vitro9. This process uses multiple cycles of template denaturation, primer annealing, and primer elongation to amplify DNA sequences. It is an exponential process since the amplified products from each previous cycle serve as templates for the next cycle of amplification,
Another method for in vitro clonal amplification is bridge PCR, in which fragments are amplified upon primers attached to a solid surface and form  DNA colonies  or DNA clusters. This method is used in the Illumina Genome Analyzer sequencers.