-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
First Lady Melania Trump. First Lady of the United States Melania Trump is the wife of President Donald J. Trump, and was born on April 26, 1970 in Slovenia. Melania Knavs began her modeling career at the age of sixteen.