-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Melania Trump is the exception to her husbandâs nativist politics. By Lauren Collins. She is the ultimate embodiment of Donald Trumpâs bargain with the electorate. In July of 2002, two years before Donald Trump became engaged to the Slovenian model Melania Knauss, he visited her native country for three hours. The couple had been in London. At around 8 P.M. on a Monday night, they landed at Ljubljanaâs Brnik airport in Trumpâs Boeing 727.