IHC: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:150 with AutoProbe III (GTX28620). Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.
Symmetry arguments suggest that most ligands engage metals via multiple bonds. The term 'metal ligand multiple bond is often reserved for ligands of the type CR n and NR n (n = 0, 1, 2) and OR n (n = 0, 1) where R is H or an organic substituent, or pseudohalide.s a cautionary note, the classification of a metal ligand bond as being multiple bond order is ambiguous and even arbitrary because bond order is a formalism.
Metallic bonds. Metallic bonds fall into two categories. The first is the case in which the valence electrons are from the sp-shells of the metal ions; this bonding is quite weak. In the second category the valence electrons are from partially filled d-shells, and this bonding is quite strong.