1 Breathe in deeply through your nose, expanding your abdomen and then filling your lungs with air. 2  Hold your breath for a couple of seconds. 3  Breathe out slowly through your mouth and empty your lungs completely.  Repeat for 5 to 10 minutes.
PCR is the enzymatic amplification of a specific DNA sequence in vitro9. This process uses multiple cycles of template denaturation, primer annealing, and primer elongation to amplify DNA sequences. It is an exponential process since the amplified products from each previous cycle serve as templates for the next cycle of amplification,
385 EXPERIMENT 24 Amplification of a DNA Fragment Using Polymerase Chain Reaction Theory Polymerase chain reaction (PCR) is a techniquethat allows the amplification of a specific fragmentof double-stranded DNA in a matter of hours.ny biological sample recovered from a crime scenethat contains DNA (a single hair, a drop of blood,skin cell, saliva, etc.) can be subjected to PCR.Primers complementary to repetitive DNA se-quences found throughout the human genome arechange a single base pair in the gene of interest(Fig. 24-4 d).