Students will read excerpts from Frederick Douglassâs groundbreaking autobiography, Narrative of. the Life of Frederick Douglass, an American Slave, which provides vivid firstâhand accounts of the. lives of slaves and the cruel actions of slave owners. Students will demonstrate their understanding.
Key Concepts: Bacterial DNA polymerase I enzymes are multidomain proteins with separate polymerisation, proofreading and 5â²â3â² exonuclease functions. They perform a vital function in bacteria by carrying out DNA repair and some aspects of chromosomal replication.
Just prior to the addition of 14 N and periodically thereafter, as the bacterial cells grew and replicated, Meselson and Stahl sampled DNA for use in equilibrium density gradient centrifugation to determine how much 15 N (from the original or old DNA) versus 14 N (from the new DNA) was present.