Product managers often start with a product requirements document (PRD). A product requirements document defines the product you are about to build: It outlines the product's purpose, its features, functionalities, and behavior. Next, you share the PRD with (and seek input from) stakeholders - business and technical teams who will help build, launch or market your product.
For bacteria: The two major families of common bacteria are distinguished by the chemistry of their walls, specifically the ability of iodine to mordant their staining with crystal violet and prevent its being removed with strong solvent. Gram positives include staph and strep, while gram negatives include e. Coli.
. Water is used to decolorize. the cells; as the endospores are resistant to staining, the endospores are equally resistant to de-staining and will retain the primary dye while the vegetative cells will lose the stain.The addition of a counterstain or secondary stain (safranin) is used to stain the decolorized vegetative cells.hen vegetative cells are subjected to environmental stresses such as nutrient deprivation they eventually die. However, some bacteria such as the Bacillus spp. and the Clostridium spp can circumvent the problems associated with environmental stress by forming. endospores. .