The hydrogen peroxide-oxidation of o-phenylenediamine (OPD) catalyzed by horseradish peroxidase (HRP) at 37 Â°C in 50 mM phosphate buffer (pH 7.0) was studied by calorimetry.
The Spartan Beast is 10+ miles. The Super Spartan is 8-10 miles and the Spartan Sprint is 5k. The distances are estimate because no two courses are the same. The race I completed was the Super Spartan. The course was at Vail Lake Resort in Temecula, CA. Upon registering for the run you select start times.
It took about 20 hours to do it an hour or so each day for a few weeks including writing down the pseudonumes and memorizing them as a phantasmagoria, or series of visual images. For the first 500 digits of pi, I used the Norwegian version of the Pocket Pseudonumer to find pseudonumes.