The hydrogen peroxide-oxidation of o-phenylenediamine (OPD) catalyzed by horseradish peroxidase (HRP) at 37 Â°C in 50 mM phosphate buffer (pH 7.0) was studied by calorimetry.
The Spartan Beast is 10+ miles. The Super Spartan is 8-10 miles and the Spartan Sprint is 5k. The distances are estimate because no two courses are the same. The race I completed was the Super Spartan. The course was at Vail Lake Resort in Temecula, CA. Upon registering for the run you select start times.
Mendez trained the SPARTAN's until 2525 when, at the age of fourteen, they would go through the toughest part of their training: the biological augmentation procedures, which would kill 30 of the 75 conscripted children and cripple 12 others who would wash out of the SPARTAN-II program.