Procedure: 1  Place 100 ml of homogenizing solution in a beaker heat the solution in a water bath until it reaches 60 C. 2  Mince the onion and add to the solution when it has reached 60 C. Stir and let sit for 15 minutes.3  After the heat treatment, immediately place the beaker into an ice bath for 5 minutes. 4  Pour the contents of the beaker into the 5  ... F The phosphate groups on the outside of DNA carry a negative charge. 2  These negative charges are attracted to and are neutralized by cations such as sodium. 3  When sodium is added to DNA it forms a protective shell around it. 4  On the other hand, protein molecules precipitate from solution in the presence of salt.
In general, a rural area or countryside is a geographic area that is located outside cities and the centers of towns.BI defines rural areas as those areas with a population of less than 49,000 (tier -3 to tier-6 cities). It is generally said that the rural areas house up to 70% of Indiaâs population. Rural India contributes a big chunk to Indiaâs GDP by way of agriculture, self-employment, services, construction etc.
Metropolitan statistical area. In the United States, a metropolitan statistical area (MSA) is a geographical region with a relatively high population density at its core and close economic ties throughout the area. Such regions are neither legally incorporated as a city or town would be, nor are they legal administrative divisions like counties or separate entities such as states.