These answers are actually quite wrong. 1) Proteins are soluble and most stable when folded (laws of thermodynamics, entropy gains by folding), nobody wants to purify unfolded, catalytically inactive protein. 2) DTT does not cause unfolding. Does not. Proteins are often found in highly reducing environments, so DTT stops oxidation of the protein (especially helpful in metal containing proteins) and the formation of unwanted disulfide bonds. If your protein requires disulfide bonds then you may not want to use DTT.
A cigarette has two flavors, regular and menthol while pipe tobacco has many flavors, ranging from spicy to fruity to sweet. 3. The flavor and aroma of cigarettes are added chemically while the flavor and aroma of pipe tobacco are added while the plant is still growing by spraying them on the leaves.
Hookah smoking is not safer than cigarette smoking. Also known as a narghile, shisha or goza, a hookah is a water pipe with a smoke chamber, a bowl, a pipe and a hose. Specially made tobacco is heated, and the smoke passes through water and is then drawn through a rubber hose to a mouthpiece. The tobacco is no less toxic in a hookah pipe than in a cigarette, and the water in the hookah does not filter out the toxic ingredients in the tobacco smoke. Hookah smokers may actually inhale more tobacco smoke than cigarette smokers do because of the large volume of smoke they inhale in one smoking session, which can last as long as 60 minutes.