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What muscles does running work? Running works the quadriceps, hamstrings, gluteals, hip flexors, abs, calves, tibialis anterior and poroneal muscles. Although the exercise does not build these muscles or make them stronger, it does make them capable of greater endurance.
Primary lower-body muscles used in both sprinting and jogging include the quadriceps, hamstrings, glutes, iliopsoas and calves. Although you hit the same muscles in both exercises, you activate more muscle fibers during a sprint than you do during a long jog, leading to increased muscle growth.