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What muscles does running work? Running works the quadriceps, hamstrings, gluteals, hip flexors, abs, calves, tibialis anterior and poroneal muscles. Although the exercise does not build these muscles or make them stronger, it does make them capable of greater endurance.
Your hip flexors move your knees into your chest and also move your legs from front to back and side to side. Because many people spend the majority of their work days sitting down, the hip flexors can become tight and prone to injury. Runners should pay special attention to stretching this muscle group.