The hydrogen peroxide-oxidation of o-phenylenediamine (OPD) catalyzed by horseradish peroxidase (HRP) at 37 Â°C in 50 mM phosphate buffer (pH 7.0) was studied by calorimetry.
The Spartan Beast is 10+ miles. The Super Spartan is 8-10 miles and the Spartan Sprint is 5k. The distances are estimate because no two courses are the same. The race I completed was the Super Spartan. The course was at Vail Lake Resort in Temecula, CA. Upon registering for the run you select start times.
This skill is on of your higher damaging skills and it also does extra damage to knocked down targets so you should use Elven Rage beforehand. It does have a long cooldown of 21 â 23 seconds depending on the skill rank. It gives you a forward guard whilst using the skill and is also your 200% Black Spirit Rage skill.