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What muscles does running work? Running works the quadriceps, hamstrings, gluteals, hip flexors, abs, calves, tibialis anterior and poroneal muscles. Although the exercise does not build these muscles or make them stronger, it does make them capable of greater endurance.
And the gluteus maximus, the largest muscle in the human body, is primarily engaged only during running. âYour butt is a running muscle; you barely use it when you walk,â Dr. Lieberman said. âThere are so many features in our bodies from our heads to our toes that make us good at running.â.