The hydrogen peroxide-oxidation of o-phenylenediamine (OPD) catalyzed by horseradish peroxidase (HRP) at 37 Â°C in 50 mM phosphate buffer (pH 7.0) was studied by calorimetry.
The Spartan Beast is 10+ miles. The Super Spartan is 8-10 miles and the Spartan Sprint is 5k. The distances are estimate because no two courses are the same. The race I completed was the Super Spartan. The course was at Vail Lake Resort in Temecula, CA. Upon registering for the run you select start times.
Equipment received from the Barrows minigame is fully repaired and can be used for around 15 hours of combat (100,000 charges) until it degrades completely and requires repair before it can be used again. Barrows armour degrades even if the player is using protection prayers or not taking damage.