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What muscles does running work? Running works the quadriceps, hamstrings, gluteals, hip flexors, abs, calves, tibialis anterior and poroneal muscles. Although the exercise does not build these muscles or make them stronger, it does make them capable of greater endurance.
Hamstrings. Three muscles called the semitendinosus, semimembranosus and biceps femoris make up your hamstrings. These muscles run along the back of your thighs and are easily injured or strained. They run from your pelvis to the back of your knees and together work to stabilize the hip joint. The hamstrings are the primary muscles that perform hip extension during normal walking. In addition to hip extension, the hamstrings also bend your knees. These muscles propel your body forward and increase your walking speed.