Product managers often start with a product requirements document (PRD). A product requirements document defines the product you are about to build: It outlines the product's purpose, its features, functionalities, and behavior. Next, you share the PRD with (and seek input from) stakeholders - business and technical teams who will help build, launch or market your product.
For bacteria: The two major families of common bacteria are distinguished by the chemistry of their walls, specifically the ability of iodine to mordant their staining with crystal violet and prevent its being removed with strong solvent. Gram positives include staph and strep, while gram negatives include e. Coli.
A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope. Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain. In Gram staining, crystal violet stains only Gram-positive bacteria, and safranin counterstain is applied which stains all cells, allowing the identification of Gram-negative bacteria as well. An alternative method uses dilute carbofluozide.