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What muscles does running work? Running works the quadriceps, hamstrings, gluteals, hip flexors, abs, calves, tibialis anterior and poroneal muscles. Although the exercise does not build these muscles or make them stronger, it does make them capable of greater endurance.
Photo: www.shutterstock.com. Hydration during running is not as complicated as you may have been led to believe. When you run, you sweat. The more you sweat, the more your blood volume decreases. The more your blood volume decreases, the harder your heart has to work to deliver oxygen to your working muscles.