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The brain heart infusion, peptone and dextrose components of the medium provide the nutrients to BHI Agar. Organic nitrogen, carbon, sulfur, vitamins and trace substances are provided by the peptones and infusion. Dextrose provides the carbohydrate source for fermentative microorganisms.
Characteristics of the components used in nutrient agar/broth. 1  Beef extract is an aqueous extract of lean beef tissues. 2  Peptone is made by digesting proteinaceous materials e.g., meat, casein, gelatin, using acids or enzymes. 3  Agar is a complex carbohydrate obtained from certain marine algae. It is used as a solidifying agent for media and does not have any nutritive value. Agar gels when the temperature of media reaches 45Â°C and melts when the temperature reaches 95 Â°C. Preparation of Nutrient Agar