These answers are actually quite wrong. 1) Proteins are soluble and most stable when folded (laws of thermodynamics, entropy gains by folding), nobody wants to purify unfolded, catalytically inactive protein. 2) DTT does not cause unfolding. Does not. Proteins are often found in highly reducing environments, so DTT stops oxidation of the protein (especially helpful in metal containing proteins) and the formation of unwanted disulfide bonds. If your protein requires disulfide bonds then you may not want to use DTT.
A cigarette has two flavors, regular and menthol while pipe tobacco has many flavors, ranging from spicy to fruity to sweet. 3. The flavor and aroma of cigarettes are added chemically while the flavor and aroma of pipe tobacco are added while the plant is still growing by spraying them on the leaves.
Chewing tobacco. Any leaf tobacco that is not intended to be smoked. Pipe tobacco. Any tobacco which, because of its appearance, type, packaging, or labeling, is suitable for use and likely to be offered to, or purchased by, consumers as tobacco to be smoked in a pipe.