Fluorite Properties Fluorite is made up of calcium fluoride and has a transparent or translucent appearance. Sometimes it can be a colorless crystal, but other times it can also be purple, blue, green, yellow, or pink, or other combinations of these colors. Fluorite is sometimes called fluorspar. The name is derived from the Latin word âfluoâ, meaning to flow.
A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light.These parameters are used to identify the presence and the amount of specific molecules in a medium.he machine is constructed like this to decrease the stray light from the upper beam that may strike the detector. The optimal angle is 90 degrees. There are two different approaches to handling the selection of incident light that gives way to different types fluorometers.
They measure the absorbance, which is known as the optical density (OD), on a spectrophotometer. One can tell from the OD whether the bacteria are actively dividing or whether they are starting to die. Spectrophotometers use a light source to shine an array of wavelengths through a monochromator.This device then transmits a narrow band of light, and the spectrophotometer compares the light intensity passing through the sample to that passing through a reference compound.hey measure the absorbance, which is known as the optical density (OD), on a spectrophotometer. One can tell from the OD whether the bacteria are actively dividing or whether they are starting to die. Spectrophotometers use a light source to shine an array of wavelengths through a monochromator.