Students will read excerpts from Frederick Douglassâs groundbreaking autobiography, Narrative of. the Life of Frederick Douglass, an American Slave, which provides vivid firstâhand accounts of the. lives of slaves and the cruel actions of slave owners. Students will demonstrate their understanding.
Key Concepts: Bacterial DNA polymerase I enzymes are multidomain proteins with separate polymerisation, proofreading and 5â²â3â² exonuclease functions. They perform a vital function in bacteria by carrying out DNA repair and some aspects of chromosomal replication.
Background Information. In order to use your Addgene plasmid, you will need to isolate plasmid DNA from the bacterial cells. To do this, you will lyse the host bacterial cells and separate the proteins, RNA and chromosomal DNA from the plasmid DNA.This procedure is generally referred to as a mini-prep.ackground Information. In order to use your Addgene plasmid, you will need to isolate plasmid DNA from the bacterial cells. To do this, you will lyse the host bacterial cells and separate the proteins, RNA and chromosomal DNA from the plasmid DNA.