The hydrogen peroxide-oxidation of o-phenylenediamine (OPD) catalyzed by horseradish peroxidase (HRP) at 37 Â°C in 50 mM phosphate buffer (pH 7.0) was studied by calorimetry.
The Spartan Beast is 10+ miles. The Super Spartan is 8-10 miles and the Spartan Sprint is 5k. The distances are estimate because no two courses are the same. The race I completed was the Super Spartan. The course was at Vail Lake Resort in Temecula, CA. Upon registering for the run you select start times.
Minivans are considerably more expensive than they once were. Though some offer spartan models starting in the low-$20,000 range, most start in the upper $20,000s. From there, slathering on equipment can leave luxury-lined models passing the $45,000 mark.