I actually tweaked this recipe a bit because I needed to use up some sour cream, so I substituted the whole milk yogurt with a 3/4 cup sour cream and 1/4 cup orange yoplait yogurt. Then I used orange zest and orange juice instead of lemon.
In general terms, your THR is 60-80% of your maximum heart rate. The Karvonen Method of calculating THR is one of the most effective methods to determine target heart rate because it takes into account resting heart rate. Ad.
Reverse transcription PCR (RT-PCR) is a modification of the standard PCR technique that can be used to amplify mRNA. The first step is to convert isolated mRNA to a complementary DNA (cDNA) molecule using an RNA-dependent DNA polymerase (also known as reverse transcriptase) during a process called reverse transcription (RT).