With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
1/4. Introduction. The quantification of DNA or RNA by the spectroscopy is a well-established routine method in many. laboratories. The absorbance of UV light at wavelength of 260 nm by a pure DNA or RNA provides a. simple and accurate estimation of the concentration of nucleic acids in a sample. For example, the. absorption of 1 OD (A) is approximately equivalent to 50 Âµg/ml dsDNA, 33 Âµg/ml ssDNA, 40 Âµg/ml. RNA or 30 Âµg/ml for oligonucleotides. Besides, the quality of a DNA or RNA sample can also be checked by the spectroscopy.