With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
Since DNA methylation analyses are just getting started after bisulfite conversion, itâs useful to first understand what just happened to your sample during the conversion. Bisulfite conversion is a relatively harsh process that will dramatically change the chemical and physical properties of your DNA sample. During the process, your genomic DNA transitions from large and stable double-strand molecules to an assortment of randomly fragmented single-stranded molecules.