Use the chart below for amounts. Paste up your dye with the urea water (see below), then add rest of water and stir 'till thoroughly dissolved. Pour into squeeze bottles with a funnel. An already wet, tied up adult tee will absorb about 4 oz of liquid dye, depending on how much you apply. Use this as a guide to help you decide how much to mix up.
Bdellovibrio bacteriovorus is a small, motile, deltaproteobacterium that is a predatory invader of other gram-negative bacteria. It occupies an intraperiplasmic niche and kills, digests, and lyses its host, the prey cell. The predatory (host-dependent, or HD) process is summarized in Figure 1.
More than 35 years later, Bdellovibrio biology is undergoing a revival owing to the original, pioneering genetics work of Thomashow and colleagues (13), which has been followed up by genome sequencing and the development of new gene inactivation methods to study the function of these bacteria (32, 42, 60).