Itâs easy to understand how insomnia might be linked to depression. âChronic sleep loss can lead to a loss of pleasure in life, one of the hallmarks of depression,â explains Stanford University research psychologist Tracy Kuo, PhD. âWhen people canât sleep, they often become anxious about not sleeping.
There is a clear need to design an improved controllable plasmid attachment system to add functional moieties to plasmid DNA in order to monitor and improve gene delivery and also for DNA vaccination to improve immunogenicity. We have devised an improved system based on ODNs of 100% locked nucleic acid (LNA) . LNA is an analogue of RNA or DNA. The term LNA is used to describe both nucleotide monomers, in which the ribose ring is constrained by a methylene linkage between the 2â²âoxygen and the 4â²âcarbon, and also ODNs that contain one or more monomers of LNA.
The PCR primer specificity provides a mechanism to enrich for mutation detection, and thereby provides enhanced sensitivities (LOD of 1â5% for mutant sequences) compared with other methods. Table 4 provides a summary of these and other methods used for analysis of KRAS mutation status.