If you took the long trip with Barbas to Haemar's Shame you will have fought an awful lot of enemies with Barbasâ help. If you fast-traveled there, you will find Barbas in the inner depths of the cavern, at the foot of the Shrine of Clavicus Vile, busy killing the last of the vampire worshippers that inhabit the cave.
Excessive masturbation can cause chroinc prostatitis, so please reduce the times of masturbation. What's more, if you also have other symptoms as freqeunt or urgent urination, or testicular pain,....
All sorts of myths exist around masturbation, most of which were created by religious banter and cultural historical beliefs, and not by true scientific facts and research. To answer your question more specifically, masturbation in fact does not affect significantly the amount of testosterone your body produces.