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Criteria for the formation of a salt bridge. A salt bridge is considered to be formed if the distance between any of the oxygen atoms of acidic residues and the nitrogen atoms of basic residues are within the cut-off distance (default 3.2 Angstroms) in at least one frame.The default distance cut-off can be changed by the user.he search can be restricted to a selection and/or a frame range given by the user. A salt bridge is considered to be formed if the distance between any of the oxygen atoms of acidic residues and the nitrogen atoms of basic residues are within the cut-off distance (default 3.2 Angstroms) in at least one frame.
Nitrate nitrogen (NO3-N) and ammonium nitrogen (NH4-N) are primary indicators of water quality. Ammonia: comes from sewage and landfill leachate. It is very toxic to aquatic life.