On the one hand, this type of organizational structure benefits from having individuals entirely dedicated to one function. In some cases, there can be cost savings and efficiency gains in combining functions in this way. However, this type of organizational structure is prone to conflict.
The alpha subunit (RpoA) initiates RNAP assembly by dimerising to form a platform on which the beta subunits can interact. The alpha subunit consists of a N-terminal domain (NTD) and a C-terminal domain (CTD), connected by a short linker.
In bacteria, transcription begins with the binding of RNA polymerase to the promoter in DNA. RNA polymerase is a core enzyme consisting of five subunits: 2 Î± subunits, 1 Î² subunit, 1 Î²' subunit, and 1 Ï subunit.uring transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. Transcription proceeds in the following general steps: 1  One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA.