Increase the salt concentration to elute the bound proteins. It is best to use a salt gradient to gradually elute proteins with different ionic strengths. At the end bump the system with a very high salt concentration (2-3M) to make sure all proteins are off the column. 4. Remove salts. Use dialysis to remove the salts from your protein solution.
See a physical therapist, chiropractor, or osteopathic doctor for this type of care. Ask your doctor if you can take acetaminophen (such as Tylenol). Ibuprofen or another anti-inflammatory medicine can also help relieve pain. To treat chronic neck pain, your doctor may prescribe medicine to relax your neck muscles.
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