What does it mean for a car to be Shackled up? just heard a song on the radio -- it had the line about a shackeled up GTO -- was just wondering -- What does it mean for a car to be Shackled up .. Thanks for any good or bad answers.. Update: Her Daddy didn't like me much, In my shackled up GTO.
The behavior of a temperature-sensitive cdc mutant. (A) At the permissive (low) temperature, the cells divide normally and are found in all phases of the cycle (the phase of the cell is indicated by its color).
Thus, in general, two copies of a particular gene of interest must be altered to observe the mutant phenotype. In contrast to C. albicans, C. glabrata, an important opportunistic pathogen responsible for 10-15% of symptomatic vaginal and systemic candidiasis (Redondo-Lopezet al.. glabrata is a significant cause of human disease in spite of the fact that it is phylogenetically closely related to the nonpathogenic S. cerevisiae. In this investigation, we used standard one-and two-step disruptions to generate ura3 and his3 auxotrophs in which the coding regions are deleted.