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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
NEW STRAND SYNTHESIS. Now that they are separated, the two single DNA strands can act as templates for the production of two new, complementary DNA strands. Remember that the double helix consists of two antiparallel DNA strands with complementary 5â to 3â strands running in opposite directions.he point where the DNA is separated into single strands, and where new DNA will be synthesized, is known as the replication fork. Single-strand binding proteins, or SSBs, quickly coat the newly exposed single strands. SSBs maintain the separated strands during DNA replication.