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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
Each strand can therefore serve as a template, or mold, for the synthesis of a new complementary strand. In other words, if we designate the two DNA strands as S and S Ê¹, strand S can serve as a template for making a new strand S Ê¹, while strand S Ê¹ can serve as a template for making a new strand S (Figure 6â2).