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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
As discussed in Chapter 3, DNA replication is a semiconservative process in which each parental strand serves as a template for the synthesis of a new complementary daughter strand.ction of topoisomerases during DNA replication. (A) As the two strands of template DNA unwind, the DNA ahead of the replication fork is forced to rotate in the opposite direction, causing circular molecules to become twisted around themselves.