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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
complementary strand. noun, Biochemistry. 1. either of the two chains that make up a double helix of DNA, with corresponding positions on the two chains being composed of a pair of complementary bases. 2. a section of one nucleic acid chain that is bonded to another by a sequence of base pairs.