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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
During DNA replication, each parent strand acts as a template for the synthesis of the other strand by way of complementary base pairing. Complementary base pairing (def) refers to DNA nucleotides with the base adenine only forming hydrogen bonds with nucleotides having the base thymine (A-T).