The basic steps are: 1  Cut open the plasmid and paste in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA). 2  Transform the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.
Boric acid (which is not the same as borax) is known to be especially toxic to infants as their systems can eliminate it only slowly. It should not be used even in solution as a home remedy for childhood infections. Some people use boric acid for yeast infections.
It doesnât break down, ever. If too much is used, borax will kill not only the creeping Charlie, but also the grass and other plants in the area. Since borax does not degrade, if too much is used it will poison the earth and nothing will grow in that spot for many, many years.t has often been suggested that boron can be used to kill creeping Charlie. Boron, or borax, is a naturally occurring crystalline mineral salt that was formed millions of years ago in the beds of ancient lakes. However, the disadvantages of using boron may outweigh the advantages.