Jay's debut album on Nu-Groove, Stay Awhile, rocked the Contemporary Jazz Charts for over a year. Soto returns in 2009 with his sophomore release, Mesmerized, produced by Darren Rahn and Grammy winner Michael Broening.
A primer is a short nucleic acid sequence that provides a starting point for DNA synthesis. In living organisms, primers are short strands of RNA. A primer must be synthesized by an enzyme called primase, which is a type of RNA polymerase, before DNA replication can occur.
Template DNA. This is the DNA that you design your primers to. It is the DNA that your polymerase will read and copy. Your template DNA can be genomic, plasmid or cDNA, but whatever your source quality counts.The more intact and purer your template DNA the easier it is to get good PCR results.ypically, DNA polymerase can copy 1,000 base pairs per minute. Therefore you need to allow at least 1 minute of extension time per 1,000 bases. At the end of this incubation new double-stranded pieces of DNA will have been created, consisting of both template and new DNA.