The basic steps are: 1  Cut open the plasmid and paste in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA). 2  Transform the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.
Boric acid (which is not the same as borax) is known to be especially toxic to infants as their systems can eliminate it only slowly. It should not be used even in solution as a home remedy for childhood infections. Some people use boric acid for yeast infections.
If too much is used, borax will kill not only the creeping Charlie, but also the grass and other plants in the area. Since borax does not degrade, if too much is used it will poison the earth and nothing will grow in that spot for many, many years.f creeping Charlie is encroaching on your yard from a neighborâs lawn and it isnât yet widespread, you can control creeping Charlie just by consistently pulling up and discarding all parts of the plant.