What does it mean for a car to be Shackled up? just heard a song on the radio -- it had the line about a shackeled up GTO -- was just wondering -- What does it mean for a car to be Shackled up .. Thanks for any good or bad answers.. Update: Her Daddy didn't like me much, In my shackled up GTO.
The behavior of a temperature-sensitive cdc mutant. (A) At the permissive (low) temperature, the cells divide normally and are found in all phases of the cycle (the phase of the cell is indicated by its color).
Unlike the rpsL counterselection, the mutants pheS phenotype is dominant meaning that the presence of the mutant gene will still cause toxicity when the wild-type version is also present. This strategy has been used in both Gram-negative and Gram-positive bacteria.