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Making the world better, one answer at a time. I think both phosphorylases as well water molecules (hydrolysis) can break those P-O-P bonds in ATP.
ATP is hydrolyzed into ADP in the following reaction: ATP+H 2 OâADP+P i +free energy. Like most chemical reactions, the hydrolysis of ATP to ADP is reversible. The reverse reaction combines ADP + P i to regenerate ATP from ADP. Since ATP hydrolysis releases energy, ATP synthesis must require an input of free energy. ADP is combined with a phosphate to form ATP in the following reaction: ADP+P i +free energyâATP+H 2 O. ATP and Energy Coupling