4. Organisms may be harvested either from the blood agar or Spectrum agar sections of the plate. Do not push wand. into agar or drag across surface. If colonies are smaller than 1 mm, touch ten instead of five. For very small pinpoint. colonies use an alternative method or perform a direct sensitivity.
Frozen: 180 calories (all carbs) for a 4 oz serving. Considering the average margarita glass holds 12 oz you're looking at 540 calories. There are about 218 calories in a frozen margarita. Frozen: 180 calories (all carbs) for a 4 oz serving. Considering the average margarita glass holds 12 oz you're looking at 540 calories. <br />There are about 218 calories in a frozen margarita.
How many calories in Rum? 56 calories a shot glass   that answer is a little misleading for a 2.5ounce shot of rum/vodka/gin... there is an average of 100-150 calories per shot. There is an average â¦of 50 calories in a 1ounce shot which is something that you will not run into in a bar or restaurant. 14 people found this useful.