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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
Difference between Leading strand and Lagging strand. The DNA replication process is generally referred to as discontinuous, because the polymerizing enzyme can add nucleotides only in the 5â-3â direction, synthesis in one strand(leading strand is continuous in the 5â-3â direction towards the fork. In the other strand (lagging strand), as the forks opens, multiple sites of initiation are exposed.