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One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy.
Each double stranded DNA molecule holds the same genetic information. Therefore each strand can serve as a template for the construction of a new strand. The template (original) strands are separated and preserved, while the new strands are assembled from nucleotides.