
 n - acetyl - l - cysteine ( nac ) is an endogenous aminothiol present both in human plasma and in urine .
n-(2-mercaptopropionyl)glycine ( mpg ) , also known as tiopronin , is a synthetic aminothiol antioxidant .
nac has been in clinical use for more than 40 years , primarily as a mucolytic agent in a variety of respiratory illness .
intravenous and oral administration of nac have been extensively used in the management of paracetamol ( acetaminophen ) poisoning .
mpg is primarily used in the treatment of cystinuria , but studies have shown that mpg can be used as a chelating , cardioprotecting , and radioprotecting agent , as well as an antidote to heavy metal poisoning . a number of electrochemical [ 59 ] , fluorometric [ 1012 ] , chemiluminescence [ 1315 ] , and liquid chromatographic [ 1618 ] methods have been developed for the determination of nac and mpg in biological samples and pharmaceuticals .
spectrophotometry is the most widely used technique in pharmaceutical analysis because it is simple , economic , and easily available to most quality control laboratories .
spectrophotometric methods have also been reported for the determination of nac and mpg in pharmaceutical formulations [ 1925 ] .  
a coupled redox - complexation reaction has been reported for the spectrophotometric analysis of nac and mpg using 1,10-phenanthroline as the chromogenic reagent . in the present work ,
we report a simple and cost - effective spectrophotometric method for the reliable analysis of nac and mpg in pharmaceutical formulations .
the method is also based on the coupled redox - complexation reaction between nac or mpg and fe(iii ) but uses 2,4,6-tripyridyl - s - triazine ( tptz ) as the chromogenic reagent .
collins et al . have introduced tptz as chromogenic reagent for determination of fe(ii ) .
the fe(ii ) complex with tptz has a twice higher molar absorptivity coefficient ( 2.2  10  l  mol  cm ) than the fe(ii ) complex with 1,10-phenanthroline ( 1.1  10  l  mol  cm ) .
all spectrophotometric studies were carried out on an ultraviolet - visible , double - beam spectrophotometer ( uv-1601 shimadzu , kyoto , japan ) , and using 1  cm quartz cells .
measurements of ph were carried out with a mettler toledo sevenmulti potentiometer ( mettler toledo , schwerzenbach , switzerland ) equipped with a combined glass electrode mettler toledo in lab 413 .
a thermostated water bath ( mgw lauda , germany ) was used to keep a constant cuvette temperature of 25  0.5c .
all chemicals were of analytical - reagent grade , and solutions were prepared in milliq deionised water .
separate 10  mm stock solutions of nac and mpg were prepared by dissolving 163.2  mg ( 1  mmol ) of nac ( merck , darmstadt , germany ) or 163.2  mg ( 1  mmol ) of mpg ( sigma - aldrich , st .
louis , usa ) in deionised water up to 100.0  ml volume and stored in the dark bottle at 4c .
dilutions were prepared daily in deionised water .   stock solution of fe(iii ) ( 10  mm ) was prepared by dissolving 270.3  mg ( 1  mmol ) of fecl3  6 h2o ( kemika , zagreb , croatia ) in 50.0  ml deionised water .
then 0.5  ml of concentrated hcl was added and the volume was adjusted to 100.0  ml with deionised water . a stock solution of 10  mm tptz ( merck , darmstadt , germany )
was prepared by dissolving 312.3  mg ( 1  mmol ) tptz in 2.0  ml of a 6.0  m hcl , followed by addition of deionised water up to a total volume of 100.0  ml .  
m ) was used to cover the ph range 3.24.0 . for solutions of ph 1.0 and 2.0 ,
two different pharmaceutical formulations of nac were analysed by the present spectrophotometric method , that is , fluimukan 200  mg granules , and fluimukan akut 600  mg dispersible tablets ( lek , ljubljana , slovenia ) .
an accurately weighed portion of the powder containing about 200  mg of nac was transferred into a 500  ml volumetric flask , and nac was dissolved in and diluted to the nominal volume with deionised water .
ten tablets of the mpg - containing drug captimer ( mit gesundheit gmbh , germany ) were weighed and pulverised .
a powder quantity equivalent to 100  mg of mpg was dissolved in 300  ml of deionised water , filtered through filter paper ( blue ribbon , s&s , germany ) , and the filtrate collected in a 500  ml volumetric flask was diluted by deionised water to the nominal volume .
it is noteworthy that such solutions are not stable and should be analysed within 24 hours .
these solutions were further diluted quantitatively with water to obtain suitable concentrations for the analysis by the proposed spectrophotometric method .
acetate buffer ( 20.0  ml , ph 3.6 ) was pipetted into a 25.0  ml calibrated flask . then 1.25  ml of 10.0  mm fe(iii )
, 1.25  ml of 10.0  mm tptz , and 1.0  ml of nac or mpg solutions were added .
the flask with reaction solution was filled to the nominal volume with deionised water , mixed well , and kept at room temperature ( about 25c ) for 30  min ( mpg ) or 60  min ( nac ) .
the absorbance of the produced fe(ii)-tptz complex was measured at  = 593  nm against a blank solution , prepared in the same manner with 1.0  ml water instead of 1.0  ml sample solution .
nac and mpg concentrations in pharmaceutical preparations were determined by using daily prepared calibration curves .
the eleven solution of every analyte were prepared for the concentration range from 1.0  m to 100.0  m .
the proposed method is based on the coupled redox - complexation reaction . in the first ( redox ) step of the reaction ( see ( 1 ) ) , rsh compound ( nac or mpg ) reduces fe(iii ) to fe(ii ) whereas rsh molecules themselves oxidize to thiyl radicals rs which combine to form the disulfide rssr . in the second step of the reaction ( see ( 2 ) ) , in situ formed fe(ii ) is immediately complexed by 2 molecules of tptz to form the deep - blue coloured , highly stable fe(tptz)2 
  complex which absorbs light at  
 max at 593  nm .
the net overall reaction can be expressed by reaction ( 3 ) : 
 ( 1)fe3++rshfe2++h++rs 
 ( 2)fe2++2  tptzfe(tptz)22 + 
 ( 3)2  fe3++2  rsh+4  tptz2  fe(tptz)22++rssr+2  h+ 
  krishnamurti and huang have reported that the complexation of tptz is specific for fe(ii ) so that this reaction can be performed in the presence of large amounts of fe(iii ) .
the results of the present study confirm these previous results for the drugs nac and mpg serving as the reducing agents .   in the literature , we were not able to find the standard reduction potential for nac and mpg .
however , the calculated formal potential of the fe(iii)/fe(ii ) couple of 0.578  v , equations ( 4 ) and ( 5 ) indicate that its oxidizing power in solution with tptz is more negative than in solution with 1,10-phenanthroline ( 1.197  v ) .
this means that the proposed method with the tptz is selective for the determination of nac and mpg .
thiols or other reducing substances with standard ( formal ) potentials higher than 0.6  v would not interfere in the proposed method ( see ( 6 ) ) ;  
 ( 4)e10=efe3+/fe2 + 00.05922log  ( fe2+fe3+[tptz]2)2 , 
 ( 5)e10=0.771  v0.0592log  1.2651050.077(3104)2=0.578  v 
 ( 6)e20=erssr / rsh00.0592ph . 
  in previous work
, we found that the initial redox - complexation reaction rate is higher with mpg compared to nac [ 28 , 29 ] . in the coupled redox - complexation reaction with mpg , the steady state value of the absorbance is reached after 30  min while in the reaction with nac the steady state value of the absorbance is reached after 60  min . with both thiols ,
.   equation ( 6 ) indicates that the potential for the redox system rsh / rssr depends upon the ph value of the reaction mixture .
the effect of the ph was therefore investigated over the range 1.04.0 using 0.1  m hcl for ph 1 , 0.01  m hcl for ph 2 , and acetate buffer for the ph values 3.2 , 3.5 and 3.6 . in this experiment mpg
the absorbance at 593  nm increased with increasing ph up to the value of 3.6 . however , precipitation of iron hydroxide occurred at the ph above 3.8 .
therefore , a buffered reaction medium of ph 3.6 was chosen as a compromise for keeping fe(iii ) in solution and achieving quantitative formation of the fe(tptz)2 
  complex which is stable in the ph range 3.45.8 .
the influence of the fe(iii ) concentration on the determination of nac and mpg at the fixed concentration of 40  m each was studied in the concentration range from 20  m to 400  m , allowing a molar ratio fe(iii)/rsh from 0.5 to 10 . in figure 2 , the absorbance measured at 593  nm is plotted versus the molar fe(iii)/rsh ratio for nac and mpg .
figure 2 shows that the reaction can be forced to completion by increasing the fe(iii)/rsh ratio , for instance by increasing the fe(iii ) concentration .  
the influence of the tptz concentration on the analysis of nac and mpg at the fixed concentration of 40  m rsh compound was studied in the range from 20  m to 400  m allowing a tptz / rsh molar ratio of 0.5 to 10 .  
figure 3 shows that absorbance increased with increasing tptz / rsh molar ratio , that is , with increasing tptz concentration , to reach its maximal value at a molar excess of five .
the effect of the reaction temperature on the signal intensity was examined by varying the temperature from 25c to 40c using the thermostated water pump .
we found that the reaction rate increased by elevating reaction temperature ( see [ 28 , 29 ] ) .
since the proposed method is an equilibrium method , signal is recorded when the reaction reaches the state of equilibrium .
the signal intensity in the state of equilibrium is the same for all the examined temperatures .
the linearity of the method was investigated under the optimized conditions for nac and mpg in the concentration range from 1.0 to 100.0  m .
straight lines were obtained from linear regression analysis of the absorbance at 593  nm and the drug concentration ( table 1 ) .
the lowest quantifiable concentration of nac and mpg by this method was 1.0  m each .
the effect of some possible interfering cations and anions on the analysis of a fixed concentration of 40.0  m for nac and mpg was investigated for the maximum molar ratio of foreign ions .
the influence of excipients that can commonly accompany nac and mpg in pharmaceutical formulations was also studied .  
the tolerance is defined as the foreign - ion / excipient concentration causing an error smaller than 5% for the determination of the analyte of interest .
the tolerable concentration of kno3 and na2so4 was 40.0  mm ( molar ratio , 1000  :  1 ) .
the tolerable concentration of glucose , fructose , sucrose , boric acid , and acetic acid was 20.0  mm ( molar ratio , 500  :  1 ) .
thus , the commonly excipients glucose , fructose , and sucrose do not interfere with the analysis of nac and mpg because they essentially do not react with the oxidizing agents .
it should be emphasized that the contaminant / analyte concentration ratios studied in the present work are much higher than those normally found in commercial pharmaceutical products .
the tolerable concentration of some other thiols , that is , d - penicillamine , l - glutathione , and l - cysteine , was 40.0  m ( molar ratio , 1  :  1 ) .
thiols or other reducing substances with standard ( formal ) potentials higher than 0.6  v would not interfere in the proposed method . in order to evaluate the potential of the proposed method to the analysis of real samples , the method was applied to three pharmaceutical formulations of the drugs nac and mpg .
the results of these analyses are presented in tables 2 and 3 .   for comparison , the spectrophotometric method reported by raggi et al .  
these authors used 1,10-phenantroline as an chromogenic reagent , instead of tptz which is used in the present work .
as shown in table 2 , there were no significant differences between the values obtained by the reported method   and those obtained by the proposed method ( p > 0.1 , student t - test ) .
this actually suggests that the proposed method is accurate and precise as the earlier reported method .
the accuracy of the method was further ascertained through the recovery studies . to the drug solutions of the granules or the tablet powder , the standard solutions of the synthetic nac or mpg
these results indicate that the proposed method is accurate for the determination of nac and mpg in their commercially available pharmaceutical preparations without any significant interference by common pharmaceutical excipients which do not absorb light in the visible region .  
performance characteristic of existing equilibrium spectrophotometric methods [ 19 , 2124 ] and the proposed method are compared in table 4 .
the proposed method is free from drastic experimental conditions such as heating unlike some of reported methods .
some other thiol compounds do not interfere in the proposed method at molar ratio 1  :  1 .
it is also worth mentioning that the proposed method was performed in the visible region (  = 593  nm ) away from the uv - absorbance of the uv - absorbing interfering excipient materials , which might be dissolved from pharmaceutical formulations .
undoubtedly , hplc is one of the most widely used techniques in routine analysis of pharmaceuticals , but it involves expensive instrumental set which many laboratories in developing and underdeveloped countries can not afford . the proposed equilibrium spectrophotometric method based on the coupled redox - complexation reaction of the thiol drug nac or mpg with fe(iii ) and tptz can be applied in every analytical laboratory as a reliable method for the determination of nac or mpg in pharmaceutical preparations . due to the use of tptz
the coloured fe(tptz)2 
  complex is stable in an extended period of time up to 24 hours .
regular excipients and additives present in the pharmaceutical preparations of nac and mpg do not interfere in this method .
 a simple spectrophotometric method for the determination of 
 n - acetyl - l - cysteine ( nac ) and 
 n-(2-mercaptopropionyl)glycine ( mpg ) in pharmaceutical preparations was developed , validated , and used . 
 the proposed equilibrium method is based on a coupled two - step redox and complexation reaction . in the first step , fe(iii ) 
 is reduced to fe(ii ) by nac or mpg . 
 subsequently , fe(ii ) is complexed with 2,4,6-tripyridyl - s - triazine ( tptz ) . 
 several analytical parameters of the method were optimized for nac and mpg analysis in the concentration range from 1.0  m to 100.0  m . 
 regression analysis of the calibration data showed a good correlation coefficient ( 0.9999 ) . 
 the detection limit of the method was 0.14  m for nac and 0.13  m for mpg . 
 the method was successfully applied to quantify nac and mpg in pharmaceutical preparations . 
 no interferences were observed from common pharmaceutical excipients . 
1. Introduction
2. Material and Methods
3. Results and Discussion
4. Conclusion