the maxillary anterior teeth should be positioned as close as possible to the positions originally occupied by natural teeth to achieve a natural appearance in making complete dentures.1 it is necessary to refer to anatomic landmarks to achieve this goal.2 the incisive papilla is a small , pear shaped eminence composed of a pad of fibrous connective tissue overlying bony exit of nasopalatine blood vessels - nerves and one of the significant anatomical landmark in locating maxillary anterior central incisors position in complete denture fabrication procedure .
harper3 stated that the incisive papilla was stable that was obtained by caliper measurements on pre - extraction and post - resorption models of the same cases over seven years .
although the shape and the localization of the papilla shows a wide range of variation,58 the centre of the papilla is commonly used as a reference point in denture construction and studies.24,915 when artificial teeth is set in proper positions , which were determined from the incisive papilla , the foundation is correctly laid for natural speech , pleasing appearances and normal function.3 the horizontal relationship between incisive papilla and maxillary central incisors has been already investigated by several authors .
harper3 suggested that the incisal edges of the maxillary central incisors should be 5 to 8 mm at the horizontal direction in front of the centre of the papilla .
ortman and tsao6 stated that the most anterior part of the maxillary central incisors and the posterior of the incisive papilla was 12.45 mm .
many studies showed that incisal edges of the maxillary central incisors were positioned 8 to 10 mm in front of the centre of the incisive papilla.2,4,68,1020 another anatomical landmark that have been suggested in complete denture construction is pterygomaxillary notch - incisive papilla plane.21 fu et al22 found that the pterygomaxillary notch - incisive papilla plane tends to be more parallel to the occlusal plane that uses mesio - labial incisal edge of maxillary right central incisor as anterior reference point and mesio - buccal cusp tips of maxillary first or second molars as posterior reference point .
although , several studies investigated the horizontal relationship between incisive papilla and the maxillary central incisors,24,10,11,16,17,20,21,2325 the authors did not find specific information in the literature relative to vertical distance between the incisive papilla and the maxillary central incisors .
thus , the aim of the present study was to determine the vertical distance between the maxillary central incisors and centre of the incisive papilla .
dental student volunteers from suleyman demirel university faculty of dentistry were solicited by a written announcement to participate in the study .
the inclusion criteria were : no restoration and tooth loss on the upper jaw , well - arranged maxillary anterior teeth , no orthodontic treatment , angle class i maxillomandibular relationship , no pathology that affects the dentition or the surface texture and shape of teeth , no incisal wear on the dentition and no gummy smile in the subjects .
the volunteers were examined by one of the investigators of the study and one hundred volunteers ( 58 women , 42 men ) were selected from the students that have the inclusion criteria with a draw .
the ages of the subjects were ranged from 19 to 22 years , with a mean age of 20.371.1 years .
upper jaw impressions were taken on the subjects by using stock impression trays ( teknik dental rostfrei , istanbul , turkey ) and irreversible hydrocolloid impression material ( tulip , cavex holland , haarlem , holland ) . in order to correctly register the incisive papilla and reduce soft tissue distortion
the stone casts were obtained using ada type iii dental stone ( gilidur , fachbereich dental , ludwigshafen , germany ) .
each stone cast was trimmed following the same procedures to produce a flat base that was parallel to the occlusal plane ( anterior reference point : mesio - labial incisal edge of upper right central incisors ; posterior reference points : mesio - buccal cusp tips of upper first molars ) .
two mm standard transparent resin plate and a spirit level ( veto , istanbul , turkey ) were used to achieve the parallelism of the described occlusal plane to the base of the stone casts .
the centre of the incisive papilla was found measuring the planar distance between the most anterior and posterior borders of the incisive papilla that was on the median line of the palate using a digital caliper which had a precision level at 0.01 mm ( mitutoyo corporation , tokyo , japan ) ( figure 1 ) and halving this distance .
the midpoints of the incisive papilla were marked on each stone cast ( figure 2 ) .
a digital caliper that was fixed perpendicular to the horizontal bar of a surveyor ( rotaks dent a.s .
, istanbul , turkey ) was used as a measurement instrument ( figure 3 ) .
stone casts were mounted to the surveyor by the help of a spirit level to obtain a certain level in the horizontal plane .
the midpoint of the flat edge panel of the depth caliper was marked with graphite .
the flat edge plane of the depth caliper had contacted to the mesial incisal edges of the maxillary central incisors and the midpoint of the flat edge plane of the depth caliper was matched with the midpoint of the maxillary central incisors ( figure 4 ) .
the pin of the depth caliper was touched to the midpoint mark of the incisive papilla .
the mounted casts were moved by sliding and the first measurement accepted as a starting point .
the measurements were done by two investigators using the same instrument at two separate times .
the average values of two investigators measurements were calculated and analyzed with spss 13.0 for windows ( spss inc . ,
the kappa scores for the assessment of intra and inter observer agreement were higher than 0.75 which implies substantial agreement between the observers ( tables 1 and 2 ) .
the mean vertical distance between maxillary central incisors and midpoint of the incisive papilla on the stone casts was 6.700.81 mm .
a common average vertical position of the maxillary anterior teeth to the constant landmarks has been found by measuring lots of casts of natural healthy teeth .
it is important to orient the stone casts in a standardized manner during measurements so that the results achieved can be applied when artificial teeth are being set in complete denture bases .
the occlusal plane is a common reference for mounting stone casts and in general , occlusal plane done parallel to the bases of stone cast in standardize manner .
fu et al22 found the pterygomaxillary notch - incisive - papilla plane ( hip ) tends to be parallel to the natural occlusal plane that defined as anterior reference point was mesio - labial incisal edge of upper right central incisor and posterior reference points were mesio - buccal cusp tips of upper first or second molars . in the present study
, a standard transparent resin sheet was positioned in contact with the maxillary right central incisors and the mesio - buccal cusp tips of first molars to orient the stone cast to the standardized occlusal plane .
this occlusal plane orientation was used , for it is more comparable to the pterygomaxillary notch - incisive - papilla occlusal plane.22 harper3 stated that the incisive papilla is a dependable basis for reproducing the horizontal and vertical position of the maxillary central incisors .
watt9 stated that the anterior portion of the maxilla undergoes extensive resorptive changes following tooth removal and the anatomic location of the incisive papilla can be used only as a guide in the setting of denture teeth .
grave16 suggested that using the border of the incisive papilla as the part likely to be least affected by the changes in anatomy of the region . in the previous studies , the midpoint of the incisive papilla has been used as a reference point for tooth arrangement .
most of the previous studies used the midpoint of the incisive papilla as a reference point.2,4,1015,25,26 the midpoint of the incisive papilla has also been used as a reference point for tooth arrangement and occlusion rims in contemporary dental practice .
pterygomaxillary notch - incisive - papilla occlusal plane also uses the centre of the incisive papilla as anterior reference point.22 for these reasons ; the centre of the incisive papilla was used as a reference point for measurements in the present study .
ortman and tsao6 stated that the most anterior part of the maxillary central incisors and the posterior of the incisive papilla was 12.45 mm .
the measurement methods of all these studies were in 2-dimensional , however park et al28 found similar results ( 11.961.37 ) in their 3-dimensional measurement on a virtual model between the maxillary anterior teeth and incisive papilla .
the results of these studies that used 2 and 3 dimensional measuring methods show that a sensitive measuring method is reliable and can be repeatable .
chalsuthipan and boonsiri29 investigated the relationship between the incisive papilla , maxillary central incisors and canines in thai population .
the authors found that the vertical distance from the most distal point of the incisive papilla to incisal edge of the central incisors was ranged from 6.94 to 7.23 mm with the mean of 7.08 mm.29 the present study supports the findings of chalsuthipan and boonsiri.29 the mean value difference between chalsuthipan and boonsiri s study29 and the present study was 0.38 mm .
the mean value variation may be caused by the reference point differences on the incisive papilla because the authors used the most posterior border of the incisive papilla .
the most posterior part of incisive papilla is the most far from the occlusal plane and maxillary central incisors . on the anterior region of the maxilla ,
the vertical relationship between incisive papilla and central incisors can be used in determination of occlusal plane with the reference of other anatomical landmarks together ; however the clinical applicability of this relationship in complete denture construction needs further investigation .
this study investigated the vertical distance between maxillary central incisors and centre of incisive papilla .
the dentist and laboratory technician can be use the results of this study as a guideline in fabrication of maxillary occlusal rims and in determination of the position of the occlusal plane with the reference of other anatomical landmarks together .
however , the wax rim should be modified intraorally to incorporate individual characteristics , and the anterior tooth should be arranged on modified wax rim . also , further investigations are needed in different age , gender , race , dental and skeletal morphology groups .
 objectivesthe aim of the present study was to determine the vertical distance between maxillary central incisors and incisive papilla.methodsthe vertical distance between incisal edges of maxillary central incisors and the centre of the incisive papilla was measured by a digital caliper on the stone casts that were obtained from dentate subjects.resultsthe mean vertical distance between maxillary central incisors and incisive papilla on the stone casts was 6.700.81 mm . 
 the vertical distance range was 5.51 mm to 8.89 mm.conclusionsthe clinical relevance of this study lies in application to complete denture construction . 
 the dentist and laboratory technician can be use the results of this study as a guideline in fabrication of maxillary occlusal rims and in determination of the position of the occlusal plane with the reference of other anatomical landmarks together . 
 however , the wax rim should be modified intraorally to incorporate individual characteristics and the anterior tooth should be arranged on modified wax rim . 
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONCLUSIONS