several imaging modalities including pa , occlusal and panoramic radiography , cone beam computed tomography ( cbct ) and ct are used before and after implant treatment as well as in the maintenance sessions . after successful implant placement ,
periapical radiography is usually a suitable imaging modality for long - term follow - ups .
radiographically , a thin radiolucent margin around the implant indicates implant mobility and is an important sign of failed osseointegration [ 13 ] .
the popularity of digital radiography in dentistry is mainly due to the adjustability of digital images by using image inversion , embossed tools , brightness , contrast and magnification enhancement filters [ 49 ] . by applying image inversion to digital images ,
inverted digital images are used for various purposes in dentistry such as the measurement of bone loss due to periodontal disease and localization of the mandibular canal and mental foramen [ 10,1117 ] .
several previous studies have compared the diagnostic accuracy of unprocessed digital images ( psp and charge - coupled device or ccd ) , film - based radiographs and inverted images for detection of peri - implant bone defects and simulated periodontal lesions using image tool and adobe photoshop software programs [ 1214 ] .
this study aimed to compare the diagnostic value of unprocessed ( psp ) and inverted digital images for detection of peri - implant defects using scanora software .
soft tissue residues were removed and the rib was trimmed to prepare bone segments suitable for the placement of 30 implants . in between imaging stages , the rib segments were stored at 1c temperature to minimize moisture loss .
osteotomy sites were marked on the superior border of the rib with 15 mm distance from one another .
a total of 30 implant placement sites including 10 control sites , 10 sites with 0.425 mm peri - implant space and 10 sites with 0.725 mm peri - implant space were created .
the sicace  screw implants ( sicace , basel switzerland ) ( 3.4mm/14.5 mm ; cylindrical parallel walled ) were placed in the marked areas .
osteotomy sites were prepared in two steps . in step 1 , holes were drilled using sic drill system with 3.1 mm diameter and 14.5 mm length ( sic extension drill 3.10 mm , short ) . in step 2 of osteotomy ,
the coronal 8 mm of 10 osteotomy sites was enlarged using a 4.25 mm drill ( sic extension drill , basel , switzerland)(4.25 mm , short ) to create 0.425 mm peri - implant space .
the coronal 8 mm of another 10 sites was enlarged using a 4.85 mm diameter drill ( astra tech drill , stockholm , sweden ) ( 4.85 , 819 mm ) to create 0.725 mm peri - implant space . to stabilize fixtures in bone , drilling
was performed at a diameter of 3.1 mm and height of 6 mm for the apical part of fixtures and the remaining coronal 8 mm was prepared with larger drills to form a gap .
all fixtures measuring 3.414.5 mm ( the sicace screw implants 3.4mm/14.5 mm incl . ) were placed into the osteotomy sites at the level of the superior border of the rib ( bone level ) and cover screws were inserted ( figure 1 ) . to simulate soft tissue , an acrylic block ( polymethyl methacrylate ) with 1 cm thickness
periapical radiographs were obtained from the bone segments at the same day the fixtures were placed .
all radiographs were obtained using digora optime imaging system ( soredex corporation , helsinki , finland ) , psp size 2 sensor ( 40.0mm30.0 mm ) and minray dental x ray system ( soredex , tuusula , finland ) with exposure settings of 60 kv and 7ma for 0.16s . digital intraoral sensor holder ( kerr dental europe , bioggio , switzerland )
the x ray tube ( 2 ) the simulated soft tissue ( 3 ) bone segment with implant fixtures placed .
after exposure of sensors , images were stored in dicom files on a computer using a scanora lite software ( palodex , tuusula , finland ) with standard resolution . by applying image inversion , 60 digital images including 30 unprocessed and 30 inverted images were obtained .
four oral and maxillofacial radiologists with at least two years of work experience evaluated all 60 images for presence or absence of peri - implant defects using a efive - point scale : 
 definite defectprobable defectnot sureprobably no defectdefinitely no defect 
  examiners viewed the images on an 18.5 samsung monitor ( samsung syncmaster e1945nx , 1360 768 ) under adequate lighting .
the examiners were only allowed to adjust the brightness and contrast of digital images ( figure 3 ) .
study the basis of calculating the absolute sensitivity / specificity was the number of the definitely correct diagnoses and the complete sensitivity / specificity was determined based on the total number of definite and probable correct answers .
the sensitivity and specificity for detection of small and large peri - implant defects were calculated and compared .
independent t - test was used to compare the diagnostic value of unprocessed and inverted digital imaging modalities .
soft tissue residues were removed and the rib was trimmed to prepare bone segments suitable for the placement of 30 implants . in between imaging stages , the rib segments were stored at 1c temperature to minimize moisture loss .
osteotomy sites were marked on the superior border of the rib with 15 mm distance from one another .
a total of 30 implant placement sites including 10 control sites , 10 sites with 0.425 mm peri - implant space and 10 sites with 0.725 mm peri - implant space were created .
the sicace  screw implants ( sicace , basel switzerland ) ( 3.4mm/14.5 mm ; cylindrical parallel walled ) were placed in the marked areas .
osteotomy sites were prepared in two steps . in step 1 , holes were drilled using sic drill system with 3.1 mm diameter and 14.5 mm length ( sic extension drill 3.10 mm , short ) . in step 2 of osteotomy ,
the coronal 8 mm of 10 osteotomy sites was enlarged using a 4.25 mm drill ( sic extension drill , basel , switzerland)(4.25 mm , short ) to create 0.425 mm peri - implant space .
the coronal 8 mm of another 10 sites was enlarged using a 4.85 mm diameter drill ( astra tech drill , stockholm , sweden ) ( 4.85 , 819 mm ) to create 0.725 mm peri - implant space .
to stabilize fixtures in bone , drilling was performed at a diameter of 3.1 mm and height of 6 mm for the apical part of fixtures and the remaining coronal 8 mm was prepared with larger drills to form a gap . all fixtures measuring 3.414.5 mm ( the sicace screw implants 3.4mm/14.5 mm incl . )
were placed into the osteotomy sites at the level of the superior border of the rib ( bone level ) and cover screws were inserted ( figure 1 ) . to simulate soft tissue , an acrylic block ( polymethyl methacrylate ) with 1 cm thickness
periapical radiographs were obtained from the bone segments at the same day the fixtures were placed .
all radiographs were obtained using digora optime imaging system ( soredex corporation , helsinki , finland ) , psp size 2 sensor ( 40.0mm30.0 mm ) and minray dental x ray system ( soredex , tuusula , finland ) with exposure settings of 60 kv and 7ma for 0.16s . digital intraoral sensor holder ( kerr dental europe , bioggio , switzerland )
the x ray tube ( 2 ) the simulated soft tissue ( 3 ) bone segment with implant fixtures placed .
after exposure of sensors , images were stored in dicom files on a computer using a scanora lite software ( palodex , tuusula , finland ) with standard resolution . by applying image inversion , 60 digital images including 30 unprocessed and 30 inverted images were obtained .
four oral and maxillofacial radiologists with at least two years of work experience evaluated all 60 images for presence or absence of peri - implant defects using a efive - point scale : 
 definite defectprobable defectnot sureprobably no defectdefinitely no defect 
  examiners viewed the images on an 18.5 samsung monitor ( samsung syncmaster e1945nx , 1360 768 ) under adequate lighting .
the examiners were only allowed to adjust the brightness and contrast of digital images ( figure 3 ) .
in this study the basis of calculating the absolute sensitivity / specificity was the number of the definitely correct diagnoses and the complete sensitivity / specificity was determined based on the total number of definite and probable correct answers .
the sensitivity and specificity for detection of small and large peri - implant defects were calculated and compared .
independent t - test was used to compare the diagnostic value of unprocessed and inverted digital imaging modalities .
a total of 60 digital pa radiographs including 30 unprocessed and 30 inverted radiographs were obtained of 30 implants with the same diameter and length : 10 implants with no defects , 10 with small ( 0.425 mm ) diameter peri - implant defects in the coronal 8 mm and 10 with large ( 0.725 mm ) diameter peri - implant defects in the coronal 8 mm .
based on the results , unprocessed images had a higher diagnostic value than inverted images . according to the t - test , in the equality of the means , no significant difference was detected in the sensitivity of unprocessed and inverted images for detection of peri - implant defects ( absolute positive predictive value ) and for detection of a probable lesion ( complete positive predictive value)(p>0.05 ) .
considering the equality of variances , the t - test showed that unprocessed images had higher absolute negative predictive value ( p=0.049 ) and complete negative predictive value ( p=0.017 ) than inverted images ( table 1 ) .
the mean  standard deviation of absolute and complete negative and positive predictive values of unprocessed and inverted images the mean  standard deviation of sensitivity and specificity of unprocessed and inverted images for detection of small and large defects significantly different from zero ( p<0.05 ) considering the equality of variances , the t - test showed that unprocessed images had a higher absolute sensitivity for detection of small defects than inverted images ( p=0.48 ) .
however , the mean complete sensitivity of the two modalities for detection of small defects despite the non - equality of variances ( p=0.001 ) and equality of the means , was not significantly different ( p=0.81 ) ( table 2 ) . considering the equality of variances ( p=0.055 ) , the t - test failed to find a significant difference in the absolute sensitivity for detection of large defects between the unprocessed and inverted images ( p=1.0 ) .
however , despite the equality of variances ( p=0.003 ) , the t - test found a statistically significant difference in complete sensitivity for detection of large defects and unprocessed images had a higher mean value in this respect ( p=0.019 ) ( table 2 ) . in the non - equality of the means ,
the mean absolute specificity of unprocessed and inverted images was significantly different based on the t - test ( p<0.05 ) and unprocessed images had a higher diagnostic value .
however , in the equality of the means , the complete specificity of unprocessed and inverted images was not significantly different ( p>0.05 ) ( table 2 ) .
periapical radiography is conventionally used as the standard follow up radiography after implant placement to assess the peri - implant tissue status .
the digital imaging techniques and different enhancement filters have enabled more accurate diagnoses in dentistry .
this study showed that unprocessed images had a higher diagnostic value than inverted images for detection of small and large peri - implant defects .
this finding may be due to the unfamiliarity of the inverted images to the eyes of the observers .
our obtained results were in accord with those of kavadella et al ,   de molon et al ,   and jorgenson et al ,   despite the differences in the type of receptors ( film and digital sensor ) , type of defects and method of creating the defects .
however , our results were in contrast to those of scaf et al , in 2007 .
compared unprocessed and inverted digitized images for detection of bone loss due to periodontal disease and found no significant difference in the diagnostic value of unprocessed and inverted images .
scaf et al , in their study used pa radiographs available in the records of patients with periodontal disease .
the difference between our results and those of scaf et al . may be attributed to the different methodology and the software programs used .
kavadella et al ,   in their in - vitro study compared film - based conventional radiography with unprocessed and inverted digital radiography with ccd sensors for detection of peri - implant lesions .
our results had some differences with those of kavadella et al . in our study ,
the sensitivity and specificity of unprocessed images were higher than those of inverted digital images .
the complete sensitivity for detection of small defects was 0.90 for unprocessed and 0.62 for inverted images .
the complete sensitivity for large defects was 1 for unprocessed and 0.85 for inverted images .
. however , kavadella et al .   showed high specificity and low sensitivity values .
such differences may be due to the methodology of studies i.e. method of defect formation , using fresh bone in our study and use of magnification enhancement ( x2 ) by the observers in the study by kavadella et al .
however , our results regarding the lower accuracy of inverted digital images compared to unprocessed images were similar to those of kavadella et al .
molon et al ,   in their study compared the diagnostic accuracy of unprocessed and converted ( cmos sensor ) images with film - based conventional radiographs for detection of bone loss due to simulated periodontal disease .
the results showed that inverted digital images had lower accuracy than film - based radiographs . despite the difference between the type of sensors used in our study and the study by molon et al ,
similar results were obtained indicating the higher diagnostic accuracy of unprocessed digital images and film - based radiographs than inverted images .
jorgenson et al ,   in their comparative study evaluated the diagnostic accuracy of conventional f - speed film radiographs , unprocessed digital radiographs and inverted digital images ( psp , digora ) in patients with vertical bone defects .
the digital sensor used in our study was similar to that used by jorgenson et al , and our results were in accord with their findings .
based on the results , we found that unprocessed digital images had higher diagnostic accuracy than inverted images for detection of small and large peri - implant defects .
 objectives : this study aimed to compare the diagnostic accuracy of inverted and unprocessed digitized periapical radiographs for detection of peri - implant defects.materials and methods : a total of 30 osteotomy sites were prepared in three groups of control , study group 1 with 0.425 mm defects and study group 2 with 0.725 mm defects using the sic and astra tech drill systems with 4.25 mm and 4.85 mm diameters . 
 small and large defects were randomly created in the coronal 8 mm of 20 implant sites ; implants ( 3.4 mm diameter , 14.5 mm length ) were then placed . 
 thirty periapical ( pa ) radiographs were obtained using digora imaging system ( soredex corporation , helsinki , finland ) , size 2 photostimulable storage phosphor ( psp ) plate sensors ( 40.0mm30.0 mm ) and scanora software . 
 unprocessed images were inverted using scanora software by applying image inversion and a total of 60 images were obtained and randomly evaluated by four oral and maxillofacial radiologists . 
 data were analyzed using the t-test.results:significant differences were observed in absolute and complete sensitivity and specificity of the two imaging modalities for detection of small and large defects ( p<0.05 ) . 
 unprocessed digital images had a higher mean in terms of absolute sensitivity for detection of small defects , complete sensitivity for detection of large peri - implant defects and definite rule out of defects compared with inverted images.conclusion:unprocessed digital images have a higher diagnostic value for detection of small and large peri - implant defects and also for definite rule out of defects compared with inverted images . 
INTRODUCTION
MATERIALS AND METHODS
Preparation of bone segments:
Preparation of osteotomy sites and implant placement:
Radiographic procedure:
Radiographic evaluation:
Data analysis:
RESULTS
DISCUSSION
CONCLUSION