obesity is a worldwide phenomenon , with an incidence increasing in parallel with obesity - associated diseases such as insulin resistance , type 2 diabetes mellitus , and hypertension .
the combination of obesity and hypertension places patients at a higher risk of hypertensive end - organ damage and vascular events [ 2 , 3 ] .
blood pressure ( bp ) is subject to diurnal variation , and studies using ambulatory bp monitoring ( abpm ) have demonstrated the clinical significance of disturbances in the diurnal bp profile and have associated nondipping with a progression in end - organ damage [ 4 , 5 ] .
moreover , a nondipping pattern of bp has been reported to occur in several conditions that are frequently associated with obesity .
although body mass index ( bmi ) is associated with the severity of ambulatory hypertension and an increase in daytime bp , bmi does not reflect body fat distribution .
epicardial adipose tissue is related to visceral fat , rather than total adiposity , and shares microcirculation with myocardial tissue and coronary vessels [ 6 , 7 ] . because elevated bp is associated with ectopic fat accumulation in the intrathoracic and epicardial areas , a possibility of association of epicardial adipose tissue with hypertension as well as diurnal bp patterns [ 10 , 11 ] has been found in some recent studies [ 8 , 9 ] .
epidemiological studies have found that epicardial fat is two to three times more common among females than males and that excessive body weight increases the risk of diabetes mellitus ( dm ) and cardiovascular diseases approximately one - and - a - half to two times higher in women than it does in men .
furthermore , a higher prevalence of hypertension among overweight adults is found in women than in men , which may indicate different susceptibilities based on gender . given these facts
, we aimed to investigate the influence of obesity on the association between epicardial fat thickness ( eft ) and circadian bp changes in patients with recently diagnosed essential hypertension ( eh ) , with a gender subgroup analysis .
this cross - sectional observational single - center cohort study included patients who underwent 24 h abpm and echocardiography between january 2008 and october 2013 .
demographic characteristics recorded at the first visit included age , sex , height , weight , medication , smoking history , and other diseases .
blood was drawn for measurement of total serum cholesterol , triglycerides , high - density lipoprotein ( hdl ) and low - density lipoprotein ( ldl ) cholesterol , blood glucose , creatinine , uric acid , and high sensitivity c - reactive protein ( hs - crp ) .
body mass index ( bmi ) was calculated as the ratio of dry weight in kilograms to height squared ( in square meters ) .
a total of 441 consecutive patients , who underwent office bp measurements , 24-hour abpm , and laboratory measurements for cardiovascular risk factors and echocardiography , were analyzed .
following the recommendations of the european society of hypertension , a normotensive state was defined as a mean daytime ambulatory systolic and diastolic bp < 135/85  mmhg by abpm , associated with
true ht was assigned if the average daytime bp was higher than 135/85  mmhg and the average nighttime bp was above 120/75  mmhg .
obesity was defined as a bmi above 25  kg / m , as per the korean society for the study of obesity .
the patients with the following diseases were excluded from the study : secondary hypertension , significant liver disease , neurologic disorder , malignant disease , valvular heart disease , heart failure , and history of acute coronary syndrome , myocardial infarction , coronary revascularization procedure , carotid revascularization procedure , ischemic leg ulcer , peripheral revascularization , or amputation .
because the presence of type 2 dm is known to be associated with increased eft regardless of obesity [ 6 , 7 ] , we also excluded overt type 2 dm from the analysis to avoid its possible confounding impact on the association between eft and circadian bp variability in our hypertensive patients with or without obesity .
office bp measurements were performed using an automated device ( easy x 800 ( r / l ) , jawon medical , seoul , korea ) .
measurements were taken after patients had rested for 10  min in a sitting position , with the arm comfortably placed at the heart level .
each set of two measurements was averaged to give the office systolic and diastolic bp .
abpm was performed on each patient 's nondominant arm using an automatically oscillometric device ( tonoport v , par medizintechnik , berlin , germany ) on a normal working day .
all subjects were instructed to rest or sleep between 10:00 pm and 7:00 am ( nighttime ) and to continue their usual activities between 7:00 am and 10:00 pm ( daytime ) .
the accuracy of the device was checked against the standard auscultatory method to ensure that the difference in bp measurements between methods did not exceed 5  mmhg .
the device was set to obtain bp readings at 20 min intervals during the day ( 7:00 am10:00 pm ) and at 40 min intervals during the night ( 10:00 pm7:00 am ) .
each abpm dataset was first automatically scanned to remove artifactual readings according to preselected editing criteria .
data were edited by omitting all readings of zero , all heart rate readings < 20 or > 200 , diastolic bp readings > 150 and < 40  mmhg , systolic bp readings > 240 and < 70  mmhg , and all readings where the difference between systolic and diastolic bps was less than 10  mmhg .
the following abpm parameters were evaluated : average ambulatory 24-hour systolic and diastolic bp levels , average ambulatory daytime systolic and diastolic bp levels , average ambulatory nighttime systolic and diastolic bp levels , and mean ambulatory 24-hour , daytime , and nighttime bps .
additionally , for both sbp and dbp , the magnitude of the nocturnal decline in bp (  nocturnal decline ) was calculated as follows : daytime average bp minus nighttime average ; the percentage change in bp from day to night ( % day  night bp ) was calculated as ( daytime bp  nighttime bp ) 100/daytime bp . with this latter data , a normal dipper pattern was diagnosed when the reduction in the average sbp and dbp during the nighttime was 10% of the average daytime values . the nocturnal bp status was also assessed and expressed as either nocturnal normotension or hypertension , with nocturnal hypertension defined as a nighttime bp of 120/70  mmhg .
standard two - dimensional ( d ) and strain echocardiography were performed on all subjects while lying in the left lateral decubitus position using a 3.5 mhz transducer ( philips ie33 , philips medical systems , bothell , wa , usa ) , and the echocardiography examiners were blinded to patient information
. measurements of the thickness of interventricular septum and posterior wall , the diameter of the lv cavity , and the lv mass index ( lvmi ) were performed according to american society of echocardiography criteria .
pulsed wave doppler of the transmitral lv inflow was performed in an apical 4-chamber view with the sample volume placed at the level of the mitral valve tips , and the following measurements of global lv diastolic function were determined : peak early ( e ) and late ( a ) diastolic mitral flow velocity , and their ratio , e / a ; early ( ea ) diastolic mitral annular velocity ; deceleration time of the e wave ; and lv isovolumetric relaxation time ( ivrt ) .
echocardiographic assessments of the eft were measured perpendicularly from the free wall of the right ventricle at end - systole in three cardiac cycles according to the method previously described by iacobellis et al .  
epicardial fat was described as the echo - free space between the outer wall of the myocardium and the visceral layer of the pericardium .
the maximum eft was also measured from the point on the free wall of the right ventricle along the midline of the ultrasound beam perpendicular to the aortic annulus as the anatomic landmark ( figure 1(b ) ) and from the apical 4-chamber view focused on the right ventricle ( figure 1(c ) ) , because one of the critical issues in eft measurement is the inconsistency in the measurement location , and mean eft was averaged from the parasternal long axis , parasternal short axis , and apical 4-chamber view images .
the offline measurement of eft was performed by two cardiologists ( ik shim and ki cho ) who were unaware of the clinical data .
statistical analyses were performed with the commercially available computer program spss 18.0 for windows ( spss inc .
values are expressed as mean  standard deviation or as a percentage ( % ) .
parameter differences among the 3 groups were evaluated using the one - way anova test for normally distributed variables and the kruskal - wallis test for non - normally distributed variables .
values are expressed as means ( standard deviation ) if numerical variables or as the number of subjects and their percentages ( % ) , if categorical .
pearson correlations analysis was performed to determine factors that potentially influenced bp and hr variability .
multivariate logistic regression models for bp variability were built to determine which variables were independently associated with this status .
a total of 441 patients with eh ( male / female , 236/205 ; mean age , 50.7  13.8 ) and 83 normotensive normal weight patients ( controls , male / female , 42/41 ; mean age , 51.0  13.3 ) were analyzed , and their clinical features and ambulatory blood pressure parameters according to gender are given in table 1 ( male ) and table 2 ( female ) .
circadian bp profile and bp variability assessed by 24-hour mean bps variation were increased in hypertensive patients , especially in hypertensive obese patients , without gender difference ( all p 's < 0.05 ) . moreover , the proportion of patients with a nondipping pattern was the highest among the hypertensive obese patients .
although there were no significant differences in hr variability among the groups , hypertensive obese patients had higher daytime and 24-hour mean hr and male hypertensive obese patients also had higher nighttime hr than control patients ( all p 's < 0.05 ) .
although total cholesterol levels were higher in hypertensive obese patients of both genders than in the controls , male hypertensive obese patients had higher triglycerides and female hypertensive obese patients had lower hdl cholesterol than controls ( all p 's < 0.05 ) .
although there was no significant difference in systolic function , hypertensive patients showed significantly increased wall thickness and enlarged left atrial diameter , which were more prominent among hypertensive obese patients , without gender difference ( tables 3 and 4 ) .
interestingly , eft was higher in female than in male patients ( 7.0  2.5 versus 5.9  2.2  mm , p < 0.001 ) . among women , eft was highest in the obese eh group ( 7.5  2.6  mm )
whereas that of the control was 6.4  2.8  mm and that of the nonobese eh group was 6.7  2.8  mm ; however , eft did not vary significantly among males ( 5.9  1.9 versus 6.0  2.7 versus 5.9  2.4  mm , resp .
, p = 0.937 ) . when we compared the 24-hour abmp parameters and eft between male and female patients , female patients had significantly higher eft ( 7.0  2.6 versus 5.9  2.2  mm , p < 0.001 ) and a greater daytime bp variation ( 15.1  4.72 versus 13.6  4.45  mmhg , p < 0.001 ) than male subjects
; males had higher 24-hour mean bp ( 138.8  14.7 versus 137.3  15.3  mmhg , p = 0.043 ) and hr variability ( 15.6  7.45 versus 14.3  6.02 , p = 0.023 ) than female subjects ( table 5 ) .
variable circadian bp profiles and la size were significantly related to bmi in both females and males ( all p 's < 0.05 , figures 2(a ) and 2(b ) , table 6 ) .
however , eft was significantly correlated with 24-hour mean bp ( both day and night ) as well as variability ( mainly night ) only in female patients ( figure 2(c ) ) .
when we investigated the association of quotient of eft / bmi with bp values , eft / bmi showed positive correlation with 24-hour systolic bp ( r = 0.172 ; p = 0.009 ) , 24-hour mean bp ( r = 0.134 ; p = 0.041 ) , day systolic bp ( r = 0.150 ; p = 0.022 ) , day systolic bp variation ( r = 0.133 ; p = 0.043 ) , night systolic bp ( r = 0.217 ; p = 0.001 ) , night systolic bp variation ( r = 0.181 ; p = 0.006 ) , night diastolic bp ( r = 0.132 ; p = 0.047 ) , and night diastolic bp variation ( r = 0.179 ; p = 0.007 ) .
however , no correlation was shown between eft / bmi and 24-hour abpm parameters in male .
multivariate logistic regression analysis demonstrated that the 24-hour mean bp variability was associated with sbp ( standardized  coefficient = 0.199 ; p = 0.018 ) and eft ( standardized  coefficient = 0.175 ; p = 0.016 ) in female patients , but not in male ones ( table 7 ) .
when we performed binary logistic regression analysis to identify the independent determinants of nocturnal nondipping bp pattern , eft was independent contributor to the nondipping pattern only in female ( odds ratio 7.034 , 95% confidence interval 2.258 to 21.909 , p = 0.001 ) ( table 8) .
the most relevant information obtained from this study is that relationships among eft , obesity , and circadian bp variability are affected by gender in different manners .
although circadian bp profile and bp variability were increased in hypertensive obese patients without gender differences , female patients had significantly higher eft and greater daytime bp variability than male subjects .
bp variability was associated with sbp and eft only in female patients ; therefore , eft may be a more valuable parameter in the evaluation of bp severity and obesity in women than in men .
bmi is known to be associated with the severity of ambulatory hypertension and increased daytime bp . in the present study
, we investigated the relationship between the parameters derived from 24-hour abpm ( 24-hour sbp and dbp , and bp variability ) in nondiabetic hypertensive patients with or without obesity .
in general , bmi is correlated with bp level , and individuals with abdominal obesity have increased bp levels or are at risk for hypertension [ 3 , 5 ] . the mechanism behind obesity - related bp elevation has not been fully established .
obesity may induce autonomic dysfunction secondary to the elevation of plasma insulin concentration , because hyperinsulinemia has been associated with sympathetic activation [ 18 , 19 ] and marked increases in hr . vice versa , sympathetic hyperactivity is also an independent contributor to the insulin resistance associated with obesity [ 21 , 22 ] .
thus , it is possible that mean arterial pressure and nighttime hr might remain at daytime levels as a consequence of sustained elevation of plasma insulin concentration .
our hypertensive obese patients had higher proportions of nondipping patterns and higher daytime and 24-hour mean hr ; moreover , male hypertensive obese patients also had higher nighttime hr than the controls .
these findings are similar to the report of antic et al . , who showed that the normal dipping pattern of bp and hr is rapidly lost following a switch to a high fat diet , without affecting daytime values .
moreover , obesity suppresses nighttime parasympathetic activity and increases nighttime hr values . because diurnal variations in mean arterial pressure and hr appear to be autonomically mediated , the loss of nocturnal dipping of mean arterial pressure and hr in our obese patients must represent an underlying change in autonomic function .
since the nighttime mean bp was altered in obese patients , the presence of hypertension in obese patients might be missed by office bp measurements that were made only during the daytime .
thus , our results emphasize the importance of continuous 24-hour monitoring of cardiovascular variables , especially in obese patients .
regarding gender differences , obese females showed a higher tendency towards bp variability than obese males .
one interesting finding was that females showed a significant relationship between increased eft , obesity , and bp variability , but this was not the case in males .
still yet , there is no consensus in the literature on the impact of gender on the amount of epicardial fat .
iacobellis et al . showed bigger size of eft in males than in females , and our result showed the greater values of eft in females .
recent study by akilli et al .   showed larger eft in females than in males .
thus , these findings may indicate different gender susceptibilities related to obesity and the regional differences in fat distribution , especially eft distribution .
several studies have reported gender differences regarding the effects of obesity and change in body weight on bp [ 13 , 26 ] and cardiovascular outcome [ 12 , 27 ] .
it has been reported that obese women have a higher relative risk of diabetes and heart failure with increasing abdominal circumference than men   and that obesity has an association with diastolic dysfunction and lvmi only in women . from these results
, women can be considered to be more susceptible to cardiovascular adverse effects related to obesity . in our study , accentuated bp variability in obese hypertensive women may have indicated a role in cardiovascular vulnerability . however , the exact explanation for these gender differences is still unclear .
one possible mechanism is that such gender differences result from abrupt hormonal and body fat composition changes after menopause .
thus , obese females have a greater amount of visceral adipose tissue which may lead to insulin resistance .
insulin resistance and changes in the autonomic nervous system as a result of redistribution of body fat may have an impact on the cardiovascular system in obese women .
excess fat has traditionally been understood to be found in intra - abdominal organs such as the liver and in subcutaneous tissue .
recently , however , epicardial adipose tissue has been found to reflect visceral adiposity and has been proposed as a new cardiometabolic risk factor . increased plasma fatty acid levels
may stimulate the cardiac autonomic nervous system activity through an increase in plasma catecholamine concentrations , which may be related to impaired diurnal bp patterns .
several reports have found a relationship between bp pattern and eft [ 10 , 31 ] , and echocardiographically assessed elevated eft was independently associated with impaired diurnal blood pressure profiles in hypertensive individuals . also , increased eft has been reported to be associated with diastolic dysfunction and la dilatation due to local or systemic effects in untreated hypertensive patients . in our results , variable circadian bp profiles and la size
were significantly related tobmi in both males and females , but eft was significantly correlated with la diameter and 24-hour mean bp with variability as well as nocturnal nondipping bp pattern , only in female patients .
the present study suggests that there would be a sex - dependent regulation of bp associated with epicardial adipose tissue , and a recent study demonstrated a sex - dependent regulation of diet - induced lvh associated with sexual dimorphic expression of adipocytokines in epicardial adipose tissue .
although our patient sample seems sufficiently large compared to other studies in the literature , in this cross - sectional study we observed predictive factors and outcome variables simultaneously and had no follow - up data .
, although we suggested that the hyperinsulinemia would be the possible main cause of elevated nighttime hr levels , we did not measure the plasma insulin levels . because both pre- and postmenopausal women were included in the study , we can not assume the impact of estrogen on epicardial fat . finally , all participants were korean , and we thus can not generalize our results to populations of other ethnic groups . in conclusion ,
the relationship among eft , obesity , and circadian bp variability was affected by gender in different manners .
eft may be a more valuable parameter in the evaluation of bp severity and obesity in women than in men .
 this study aimed to investigate the effects of gender on the association between epicardial fat thickness ( eft ) and circadian blood pressure ( bp ) changes in patients with recently diagnosed essential hypertension ( eh ) . 
 a total of 441 patients with eh ( male / female : 236/205 , mean age : 50.7  13.8 ) and 83 control patients underwent 24-hour ambulatory bp monitoring and echocardiography . 
 obese eh patients had higher circadian bp profile with bp variability , wall thickness , and left ventricular mass than nonobese eh patients and controls ( all p 's < 0.05 ) without gender differences . 
 eft was higher in female than in male patients ( 7.0  2.5 versus 5.9  2.2  mm , p < 0.001 ) and higher in the obese female eh group ( 7.5  2.6  mm ) than in the control ( 6.4  2.8  mm ) or nonobese eh group ( 6.7  2.8  mm ) among women , whereas eft did not vary among males ( 5.9  1.9 versus 6.0  2.7 versus 5.9  2.4  mm , p = 0.937 ) . 
 multivariate logistic regression analysis demonstrated that the 24-hour mean bp variability was associated with sbp ( p = 0.018 ) and eft ( p = 0.016 ) in female patients , but not in male patients . 
 the relationships among circadian bp variability , obesity , and eft were affected by gender in different manners . 
 eft may be a more valuable parameter in the evaluation of bp severity and obesity in women than in men . 
1. Introduction
2. Methods
3. Results
4. Discussion