patient 1 was a 60-year - old man who was hospitalized on 5 august 2013 , because of two weeks of fever , arthralgias and malaise .
furthermore , no plasmodium parasite was observed on the giemsa stained blood smears obtained on the first day of hospitalization .
the patient had a temperature of 38.5c , low platelet count ( 6.910/l ) and hematocrit ( 39.0 % ) , but other routine blood examinations were normal .
he also had increased levels of direct bilirubin ( 6.10  mol / l ) and alanine aminotransferase ( 100  u / l ) .
the patient recalled that he had travelled to myanmar and back to china frequently , but had never received any blood transfusions or blood products .
he also recalled multiple tick bites about 2 months prior to diagnosis , particularly after outdoor activities in the jungle along border areas of china  myanmar .
patient 2 was a 30-year - old man who was hospitalized on 9 april 2013 , because of two days of fever , diaphoresis , myalgias , progressive dyspnea and fatigue .
the patient had a temperature of 38.4c , a slightly elevated leukocyte count ( 1.1110/l ) , but normal levels of other routine blood parameters .
originally from tengchong village , he had travelled to myanmar 2 months ago , returning home at the beginning of april .
he recalled multiple tick bites in the recent past and had also received blood transfusion and blood products for the treatment of renal - malaria caused by infection with plasmodium falciparum during the summer of 2012 . a nested polymerase chain reaction ( pcr ) with two sets of babesia microti
specific primers for the small subunit ribosomal rna ( ssu rrna ) was conducted on blood samples .
positive samples were tested using another set of nested pcr primers to detect the beta - tubulin gene of b. microti . to identify the clades to which the isolates belong
, a more accurate analysis of the sequence encoding the 18s rrna gene of the babesia parasite from the patients was applied with the nested pcr primers piro1f
second round pcr products that were full - length cdnas for the 18s rrna gene ( 1700  bp ) and the beta - tubulin gene ( 590  bp ) were sequenced .
the blood smears showed intraerythrocytic ring form and tetrad typical of small babesia parasites ( figure 1 ) .
sequences from ssu rrna and beta - tubulin gene obtained by direct sequencing of pcr products from the two patients were aligned by using the clustalw method ( embl - ebi , hixton , cambridge , uk ) .
those sequences were deposited in genbank with accession nos kf410825 , kj128385 and kj128387 , respectively .
the sequences of our two isolates and other sequences were clustered following the pattern described by hunfeld et al . to identify the group of our isolates .
phylogenetic trees were produced by using the neighbor joining method in mega version 5.1 ( http://mega.software.informer.com/5.1b/ ) .
the case samples ( denoted as yunnan china ) clustered with other b. microti isolates .
infection of human babesiosis has only been reported as a few sporadic cases in china . because malaria rdt was negative for each case , and the blood smears failed to find any protozoan parasites , neither patient was treated with any anti - protozoa drugs .
atovaquone , the drug of first choice for treatment of babesiosis is not available in china .
however , a combination of penicilin , quinolone and other symptomatic treatments , such as oral paracetamol tablets for the high temperature and supplemental vitamin c and vitamin b1 , were administered to these two patients .
both patients had no significant immune deficiency ; babebsia infections were relatively benign since fever and other symptoms resolved within 1 month of therapy .
the severity of human babesiosis depends on the immune status of the host and on the babesia species causing the infection , ranging from an asymptomatic infection to a severe life threatening disease . in some areas ,
b. microti is known to elicit no symptoms in about half of children and a quarter of adults .
most cases reported on b. divergens infection are severe and most of these severe babesiosis cases occurred in people who lack a spleen .
babesiosis is now classified as a notifiable disease in the united states and is recognized as an emerging health risk in other parts of the world . in asia , b. microti - like organisms have caused illness in japan , taiwan and zhejiang province of china .
importantly , human babesiosis has sometimes been diagnosed initially as malaria because of the similarity between the two diseases or the two parasites , but little is known about the prevalence of babesia spp .
infections in malaria - endemic areas , where misidentification as plasmodium infection is most probable .
myanmar border areas of yunnan , china , where is endemic area for malaria , suggesting that babesiosis and malaria co - exist in this region .
both cases presented with malaria - like symptoms ; babesia infections were confirmed by blood smears , pcr amplification and amplicon sequencing .
, their blood smears showed intraerythrocytic ring forms and tetrads , the latter forms being an uncommon finding that is considered pathognomonic of small babesia spp .
these two cases reveal that human babesiosis caused by b. microti occurs in areas along the china 
the lessons learnt from this finding are two - fold : first , diagnosis of babesiasis is usually detected by blood smear , but the ring forms of both p. falciparum and babesia spp .
previous study showed that at least 300 microscopical fields need to be reviewed to detect low level parasitemia .
the confirmation of two cases by pcr indicated that human babesiosis can be distinguished from malaria with the assistance of pcr - based diagnostics .
b. microti was regarded as a single species before , but then regarded as a genetically diverse species complex .
clade 1 of b. microti contained mostly rodent parasites and also the majority of strains thought to be zoonotic .
clade 2 contained carnivore parasites , and clade 3 contained rodent parasites that are probably not zoonotic .
as expected , the b. microti isolates reported herein belong to the clade that contains the majority of zoonotic strains .
second , babesiosis does not respond to chloroquine which might cause its misidentification as drug - resistant malaria , especially in syndemic areas .
both cases were treated with penicillin and quinolone , although these drugs have not been shown to be effective against babesiosis .
it must also be noted that most antimalarial drugs , such as chloroquine and mefloquine have no effect on babesiosis . the first regimen effective against babesiosis consists of atovaquone and azithromycin whereas the second consists of clindamycin and quinine . from the treatment standpoint ,
particularly , artemisinin - resistant p. falciparum malaria has emerged in cambodia and thailand  myanmar border . 

b. microti appears to be responsive to artemisinin derivatives , as per the study on a mouse model .
but there is still not enough data or studies to confirm that artemisinin derivatives are effective to eliminate babesia infection in humans . in conclusion ,
babesiosis and malaria are caused by pathogens with their similar morphology and overlapping symptoms , but require different treatments .
 babesiosis is a tick - borne , zoonotic disease caused by babesia spp . 
 two cases of babesiosis were detected by nested polymerase chain reaction ( pcr ) in yunnan province , china , and further confirmed by molecular assay . 
 the blood smears showed intraerythrocytic ring form and tetrads typical of small b. microti . in both cases , 
 the rapid diagnostic test ( rdt ) ruled out the possibility of co - infections with malaria . 
 neither case was initially diagnosed because of the low babesia parasitemia . 
 these two cases of babesiosis in areas along the myanmar  china border pose the question of the emergence of this under recognized infection in countries or areas where malaria is endemic . 
CASE REPORT
DISCUSSION AND CONCLUSION