chromobacterium violaceum is a gram negative , rod shaped , nonsporing , and motile bacteria that can utilize carbohydrate fermentatively and grows readily on the ordinary laboratory culture media at 35c37c.1 it naturally resides in soil and stagnant water of tropical and subtropical regions as saprophyte2 and produces a chemical antioxidant compound called violacein ( violet non - diffusible pigment).3 although first identified in 1881 , its pathogenic potential was illustrated only in 1905 by woolley from a fatal case of a buffalo in philippines.4 human case of infection caused by this pathogen was first established by lessler from malaysia in 1927.4 this organism is usually considered as nonpathogenic , but it is an opportunistic pathogen of extreme virulence for humans and animals when inoculated into bloodstream via open wound.5 several reports of serious and fatal infections have been reported worldwide , especially from asian and american countries . in majority of these cases , bacteria entered through the breached or abraded skins subsequently after exposure to contaminated soil or water.6 clinical disease caused by this organism ranges from a localized skin lesion to multiple abscesses in the vital organs and fatal septicemia.7,8 here we report a new case of bacteremia caused by c. violaceum at manmohan memorial medical college teaching hospital ( mmth ) , kathmandu , nepal . to the best of our knowledge , this is the first case of culture - proven bacteremia caused by c. violaceum from nepal . written informed consent was taken from the patient for necessary investigations and case publication in scientific journal .
a 36-year - old immunocompetent female presented at midnight of 24 august 2016 to the emergency department of mmth , a tertiary care referral hospital in kathmandu , the capital of nepal , with the clinical symptoms of fever , headache , chills , excessive sweating , burning sensation over epigastric region , and shortness of breath for the past 12 hours .
she denied any previous illness , but her husband recalled that she had previously been diagnosed with pulmonary tuberculosis and treated with isoniazid and ethambutol as a directly observed therapy 6 years back .
she lived in sitapaila , a nearby village , and spent her life as an active housewife until 3 days ago when she plucked her left toe while working in the paddy farmland .
thereafter , her left toe became inflamed and swollen with edematous ulceration . on examination , the patient was conscious and alert , but ill - looking .
local examination showed indurations above the toe region that was edematous , but not discharging pus or any serous fluid .
furthermore , she had blood pressure of 110/70 mmhg , pulse rate of 110 beats per minute , temperature of 39.6c , and respiratory rate of 22 breaths per minute .
the patient was provisionally diagnosed as a case of acute febrile illness with a suspicion of wound - related sepsis and immediate management was started .
after necessary physical examination , blood and urine samples were collected aseptically for laboratory investigations viz blood culture , urine culture , hematology parameters , widal test , and blood chemistry tests .
paracetamol ( 500 mg ) and ibuprofen ( 400 mg ) were administered by parenteral route along with electrolyte fluids . after specimen collection for laboratory investigations ,
antimicrobial regimen of 400 mg/24-hour of ciprofloxacin and 2 g/24-hour of ceftriaxone were initiated as empiric therapy for gram negative sepsis immediately after admission .
the hematological investigations showed a normal ( unremarkable ) level of total leukocyte count with adequate cellular distribution , but low hemoglobin concentration ( table 1 ) .
the clinical parameters of blood glucose , urea , electrolytes , and common liver enzymes were found normal ( table 2 ) .
widal test titer , serological test for enteric fever , was also insignificant in validating that there was no such infectious cause in the disease .
ten milliliters of blood sample was aseptically inoculated into brain heart infusion broth and incubated in aerobic atmosphere for 24 hours .
urine sample was inoculated into blood agar and macconkey agar plates semi - quantitatively and incubated aerobically for 24 hours at 37c .
blood culture broth was inspected next day , and blind subculture was made on blood agar , chocolate agar , and macconkey agar and incubated for next 24 hours .
urine culture plates were found with no growth of any bacterial species and reported as sterile .
after overnight incubation of subcultured plates from blood culture , the agar plates demonstrated numerous small colonies with a faint violet metallic pigmentation ( which later became dark violet on incubation ) with no hemolysis ( figure 1 ) .
for better demonstration of pigment , we transferred the colonies to fresh nutrient agar plate ( figure 2 ) and incubated aerobically for next 24 hours . some colonies on the nutrient agar were initially nonpigmented , but later converted to dark violet on prolonged incubation ( 72 hours ) at room temperature .
biochemical characterization of the bacteria was carried out by conventional biochemical tests such as catalase test , oxidase test , triple sugar iron agar test , citrate utilization , urea hydrolysis , sulfide indole motility , oxidation  fermentation test , methyl red - voges  proskauer test , decarboxylase tests ( lysine , arginine , and ornithine ) .
the oxidase test was performed by picking the single isolated colony with the sterile bamboo stick and rubbing over the surface of the filter paper disk impregnated with 1% tetra methyl paraphenylene diamine dihydrochloride reagent .
simultaneously , antimicrobial susceptibility test was performed by kirby - bauer disk diffusion method on mueller - hinton agar using commercially available antibiotic disks ( figures 4 ; 5 ) and the result of the susceptibility test was interpreted according to the clinical and laboratory standards institute guidelines for non - fermenting gram negatives.9 the patient was responding better after initial antimicrobial therapy , and fever subsidized after 8 hours .
based on the biochemical findings and microscopic observation ( gram staining , motility ) , the organism was confirmed as c. violaceum .
antimicrobial susceptibility test indicated that it is resistant to polymixin group of antibiotics , but susceptible to other therapeutic antibiotics ( table 4 ) .
additional blood culture was performed on day 6 to confirm the prognosis , but was negative for bacterial growth .
she was discharged on day 7 on oral ciprofloxacin ( 1,000 mg/24 hours ) and cefixime ( 500 mg/24 hours ) for next 9 days ( total 14 days ) . on followup visit ,
after completion of the therapy , she made absolute recovery without any complications and the antibiotics were discontinued .
the clinical parameters of blood glucose , urea , electrolytes , and common liver enzymes were found normal ( table 2 ) .
widal test titer , serological test for enteric fever , was also insignificant in validating that there was no such infectious cause in the disease .
ten milliliters of blood sample was aseptically inoculated into brain heart infusion broth and incubated in aerobic atmosphere for 24 hours .
urine sample was inoculated into blood agar and macconkey agar plates semi - quantitatively and incubated aerobically for 24 hours at 37c .
blood culture broth was inspected next day , and blind subculture was made on blood agar , chocolate agar , and macconkey agar and incubated for next 24 hours .
urine culture plates were found with no growth of any bacterial species and reported as sterile . after overnight incubation of subcultured plates from blood culture ,
the agar plates demonstrated numerous small colonies with a faint violet metallic pigmentation ( which later became dark violet on incubation ) with no hemolysis ( figure 1 ) .
for better demonstration of pigment , we transferred the colonies to fresh nutrient agar plate ( figure 2 ) and incubated aerobically for next 24 hours .
some colonies on the nutrient agar were initially nonpigmented , but later converted to dark violet on prolonged incubation ( 72 hours ) at room temperature .
biochemical characterization of the bacteria was carried out by conventional biochemical tests such as catalase test , oxidase test , triple sugar iron agar test , citrate utilization , urea hydrolysis , sulfide indole motility , oxidation  fermentation test , methyl red - voges  proskauer test , decarboxylase tests ( lysine , arginine , and ornithine ) .
the oxidase test was performed by picking the single isolated colony with the sterile bamboo stick and rubbing over the surface of the filter paper disk impregnated with 1% tetra methyl paraphenylene diamine dihydrochloride reagent .
simultaneously , antimicrobial susceptibility test was performed by kirby - bauer disk diffusion method on mueller - hinton agar using commercially available antibiotic disks ( figures 4 ; 5 ) and the result of the susceptibility test was interpreted according to the clinical and laboratory standards institute guidelines for non - fermenting gram negatives.9 the patient was responding better after initial antimicrobial therapy , and fever subsidized after 8 hours .
based on the biochemical findings and microscopic observation ( gram staining , motility ) , the organism was confirmed as c. violaceum .
antimicrobial susceptibility test indicated that it is resistant to polymixin group of antibiotics , but susceptible to other therapeutic antibiotics ( table 4 ) .
additional blood culture was performed on day 6 to confirm the prognosis , but was negative for bacterial growth .
she was discharged on day 7 on oral ciprofloxacin ( 1,000 mg/24 hours ) and cefixime ( 500 mg/24 hours ) for next 9 days ( total 14 days ) . on followup visit ,
after completion of the therapy , she made absolute recovery without any complications and the antibiotics were discontinued .
c. violaceum is the single species of the genus responsible for human infections.10 most clinicians are unaware of this rare bacterium despite its ubiquitous distribution.6 in the published literature , common clinical features in majority of the cases with fatal outcome appear to be sepsis , multiple liver abscesses , and diffuse pustular dermatitis.4 bacteremia associated with c. violaceum has been reported by chen et al and yang et al from taiwan,7,11 campbell et al from vietnam,5 ray et al from india,4 nanayakkara et al from sri lanka,12 and others .
most of the reported cases had the fatal outcome but successful therapeutic experience with the use of timely therapy has also been reported.5 although c. violaceum is confined to tropical countries , especially in south and southeast asia , there are only two previously reported cases from nepal . of the two cases ,
the wound sepsis case by ansari et al has demonstrated the fatality of the case while another asymptomatic bacteriuria by pant et al was self - limiting.13,14 blood culture confirmed bacteremia associated with this rare pathogen has not been reported from nepal yet . in this case ,
clinical features of the febrile woman appear to be similar to that of common enteric fever , but skin lesion and isolated bacterial strain helped in timely identification of the rare case of bacteremia . besides , previous studies suggested that the common predisposing factors for c. violaceum associated bacteremia were immunocompromised state , chronic granulomatous disease , steroid therapy , diabetes , and other systemic illnesses,6,10 but in this report there were no such factors to be noted .
consumption of contaminated water or food or through exposure of damaged skin to stagnant water or soil is a common route for transmission of this pathogen to healthy individuals .
moreover , severe infections after swimming in contaminated water , recreational or stagnant muddy water , and post - surgical cases have also been reported.6 in this case , too , there was sufficient evidence of inoculation of the pathogen and symptoms were also promising although undifferentiated . the ability of the organism to produce a peculiar metabolite violacein giving the colonies their distinctive purple color helped in proper identification of the bacteria .
nevertheless , nonpigmented strains of c. violaceum have also been involved in human infections.11,15 chromobacterium is usually susceptible to therapeutic antimicrobials , however genes associated with intrinsic resistance toward penicillins and early cephalosporins have been described.16 isolates have been found susceptible to common therapeutic antimicrobials like chloramphenicol , trimethoprim sulfamethoxazole , tetracyclines , ciprofloxacin , cefepime , and imipenem.17 therefore , broad - spectrum cephalosporins , carbapenems or fluoroquinolones can be used as an appropriate initial choice for c. violaceum infections due to the unavailability of recommended therapeutic guidelines . timely diagnosis and aggressive antimicrobial therapy would be a critical factor for an effective management of c. violaceum and reduce the high mortality associated with these infections.5,7 in our case , timely intervention with administration of broad - spectrum antibiotics to which the organism was sensitive , assisted in proper management of the illness , and the infection did not progress to septicemia and other complications .
although human infections with c. violaceum are rare , they are often associated with higher morbidity and mortality .
nevertheless , the clinical symptoms and presentation of the infection are not distinctive , it should be included in differential diagnosis of sepsis , especially if exposure to stagnant water and skin breaching is involved .
 chromobacterium violaceum is a gram negative saprophytic bacterium , prevalent in tropical and subtropical climates . 
 infections caused by c. violaceum are very uncommon , yet it can cause severe systemic infections with higher mortality when entered into the bloodstream through open wound . a case of symptomatic bacteremia in a woman caused by c. violaceum 
 was identified recently at a tertiary care teaching hospital in nepal . 
 timely diagnosis by microbiological methods and rapid administration of antimicrobials led to a successful treatment of this life - threatening infection in this case . from this experience , we suggest to include this bacterium in the differential diagnosis of sepsis , especially when abraded skin is exposed to soil or stagnant water in tropical areas . the precise antimicrobial selection and timely administration 
 should be considered when this infection is suspected . 
Introduction
Case report
Hematological findings
Blood chemistry findings
Microbiological findings
Discussion
Conclusion