with an ageing population , alzheimer 's disease ( ad ) and related neurodegenerative disorders represent a serious social and economic threat to most societies .
the cost of caring for an increasing number of people with dementia continues to rise . at present
, there are an estimated 30 million people with dementia worldwide , with numbers expected to further increase to 80 million by 2040 . the countries or regions with the largest numbers of affected individuals are china and the developing western pacific , western europe , and the unites states .
the two core histopathological hallmarks of ad are the amyloid- ( a ) plaques and the tau - containing neurofibrillary tangles ( nfts ) , both of which have been described by alois alzheimer more than a century ago .
disease - causing mutations in familial cases of ad have been described two decades ago ; yet there is still no cure for this debilitating disease .
the current treatment is limited to acetylcholine esterase ( ache ) inhibitors ( donepezil , rivastigmine , and galantamine ) and the nmda receptor antagonist , memantine ; yet neither of these strategies halts the degenerative process that characterizes ad .
what are the characteristics of the major component of the two hallmark lesions in ad and what causes the familial cases ?
the 3942 amino acid a peptide in the plaques is fibrillar , as is tau , a microtubule - associated protein that becomes hyperphosphorylated in the disease process and eventually aggregates to form nfts , neuropil threads , and the abnormal neurites that are associated with some amyloid- plaques .
although tau has been generally perceived as a neuronal protein , with mainly an axonal localization , we have recently challenged this view by demonstrating an essential dendritic function under physiological conditions and in mediating a toxicity [ 5 , 6 ] .
in addition to plaques and nfts , the ad brain is characterized by massive neuronal cell and synapse loss .
tau pathology , in the absence of overt a plaques , characterizes a series of diseases that are collectively known as tauopathies .
this includes ftld - tau ( frontotemporal lobar degeneration with tau inclusions ) with an inherited form known as ftdp-17 ( frontotemporal dementia with parkinsonism linked to chromosome 17 ) .
there is a second , major subset of ftld characterized by tau - negative yet ubiquitin - positive lesions . in this subset , the transcription and splicing factor tdp-43 ( tar dna - binding protein 43 ) has been identified as the aggregating protein , and consequently
, this form of ftld has been named ftld - tdp . similar to tau ,
tdp-43 in the aggregates is hyperphosphorylated and fragmented , a process believed to be linked to toxicity [ 811 ] . finally , in a third subset of ftld , ftld - fus , the nuclear protein fus ( fused - in - sarcoma ) has been identified as aggregated protein . while both proteins form also aggregates in subsets of amyotrophic lateral sclerosis ( als ) ,
the majority of ad cases are sporadic ( sad ) , with familial ( fad ) cases likely accounting for less than 1% . in three genes ,
autosomal dominant mutations have been identified : amyloid precursor protein ( app ) , presenilin 1 ( psen1 ) as well as presenilin 2 ( psen2 ) .
it is the amyloid precursor protein , app , from which the a peptide is derived by proteolytic cleavage .
presenilins are components of the multimeric -secretase complex that generates a. in addition to the three fad genes , apolipoprotein e ( apoe ) has been unanimously confirmed as a susceptibility gene in sporadic ad ( sad ) .
more recently , additional risk factor genes have been identified , with clu encoding clusterin , picalm the phosphatidylinositol - binding clathrin assembly protein , cr1 the complement component ( 3b/4b ) receptor 1 , and tomm40 a channel - forming subunit of the translocase of the mitochondrial outer membrane ( tom ) complex [ 1517 ] . at present , a total of ten genes , mostly with roles in the immune system and in cholesterol metabolism , are established risk genes for ad .
compared to ad , ftld is a more heterogeneous disorder , with variants that are , for example , characterized by language or behavioural problems .
ad and ftld both present with a progressive decline of memory function leading to dementia , although in ftld this is often preceded by motor symptoms such as parkinsonism .
it was several years after the first mutations had been identified in the app and psen genes in ad , that the first mutations were identified in a subset of ftld - tau , ftdp-17 , where they were found in exonic and intronic sequences of the mapt gene that encodes tau .
another subset of familial ftld is characterized by mutations in the pgrn gene that encodes the pleiotropic protein progranulin , in the vcp gene that encodes valosin - containing protein , and also in tardp encoding tdp-43 ; these ftld - tdp cases are characterized by inclusions of tdp-43 . finally , mutations in chmp2b encoding chromatin modifying protein 2b lead to ftld , in the absence of either tau or tdp-43 inclusions . in this paper
, we will be discussing a few selected tau transgenic mouse models with memory and motor phenotypes and how one of these , the k369i mutant human tau expressing k3 strain , has been employed in an enu modifier screen , outlining the strategy from breeding to gene identification .
as the focus of this review article is the application of enu mutagenesis to one of our mutant tau transgenic mouse strains , k3 , we will be comparing this particular strain with a few other tau transgenic strains we have established in the past .
mouse strains that are otherwise available in the field including those characterized by a plaque formation have been discussed by us in detail elsewhere [ 2427 ] . what has the ambition been in developing transgenic mouse strains for the tau pathology of ad in the first place ?
the aim has always been to authentically model the human pathology and to gain a deeper understanding of what causes tau pathology , how this is related to neurodegeneration and functional impairment , and ultimately , how pathogenic processes can be prevented or delayed .
when we generated the very first tau transgenic mouse model in 1995 by expressing the longest human tau isoform in mice , we reproduced a major aspect of the human pathology in a subset of neurons : somatodendritic accumulation of hyperphosphorylated forms of tau , a feature of pathological tau in ad brains .
we failed however to reproduce nft formation , neurodegeneration , and functional impairment . even by using a stronger promoter to drive human tau expression ,
this goal of the early days was not achieved , although the mice developed a motor phenotype and amyotrophy [ 29 , 30 ] . only with the identification of pathogenic mutations in ftld - tau in the mapt gene encoding tau , by expressing mutant forms such as g272v or p301l tau , we achieved nft formation , as determined by gallyas silver impregnations and electron microscopy [ 31 , 32 ] .
while we discontinued the g272v mutant mice , the p301l tau transgenic pr5 strain was employed to provide support for the amyloid cascade hypothesis that places a upstream of tau , to reveal behavioural impairments in amygdala- and hippocampus - dependent tasks [ 34 , 35 ] , to assess the role of site - specific phosphorylation and in particular of protein phosphatase 2a ( pp2a ) in this process [ 36 , 37 ] , to determine the degree of the cholinergic pathology , and to reveal an impaired stress - related unfolded protein response   and a mitochondrial dysfunction caused by pathological tau [ 4042 ] . assessing the role of mirnas in ad adds an additional level of complexity [ 43 , 44 ] .
while the pr5 mice display memory impairment as a major clinical feature of ad , another feature , parkinsonism , that characterizes a significant subset of ftld cases , has been modelled in k369i mutant tau transgenic k3 mice .
we established this strain based on the identification of the k369i mutation of tau in a single patient with pick 's disease ( pid ) , a form of ftld .
as is known for pid in general , the tau lesions in the mutation carrier 's brain showed a remarkable feature when analysed histologically : silver impregnation revealed that the tau lesions were bielschowsky - positive and gallyas - negative ; moreover , tau was phosphorylated at many epitopes but not 12e8 ( ser262/ser356 ) .
we succeeded in reproducing this distinct pathology fully in the k3 mice that express k369i mutant tau .
owing to a unique expression pattern of the k369i transgene that includes the substantia nigra , the k3 transgenic mice also modelled early - onset parkinsonism , that is , resting tremor , bradykinesia , postural instability , and gait anomalies .
they showed an increased cataleptic response to haloperidol and an early , but not late , response to l - dopa , indicating that the dopaminergic system is impaired in these mice . in recent years , impaired axonal transport has been identified as a central pathomechanism in ad [ 4651 ] .
conceptually , impaired axonal transport is linked to oxidative stress and mitochondrial dysfunction : mitochondria need to be efficiently transported along the long axonal processes for neurons to function ; furthermore they are a major source of reactive oxygen species ( ros ) [ 53 , 54 ] .
we found a selectively impaired axonal transport of distinct cargos including mitochondria and th- ( tyrosine hydroxylase- ) containing vesicles . at the molecular level
, we found that this impaired transport phenotype is due to trapping of a component of the kinesin motor machinery , the adapter protein jip1 , by elevated levels of phosphorylated tau , preventing jip1 from executing its physiological function in the axon .
a pathological interaction between tau and jip1 was further revealed in ad and not , control brain , highlighting the validity of transgenic animal models in dissecting pathomechanisms in ad .
as tau only traps jip1 when it is hyperphosphorylated , this study presents inhibition of abnormal hyperphosphorylation of tau as a promising therapeutic strategy in ad .
with the pr5 and the k3 mice we do have two complementary models at hand that present themselves for therapeutic intervention : both strains are characterized by hyperphosphorylation and increased insolubility of tau as well as silver - positive inclusions , gallyas - positive nfts in the case of r5 mice , and bielschowsky - positive inclusions predominantly resembling pick bodies in k3 mice .
the pr5 mice show an accelerated extinction in the conditioned taste aversion ( cta ) paradigm , while the k3 mice have a memory phenotype as determined by the novel object recognition test , as well as a motor phenotype that can be easily monitored either by scoring of clasping , gait , and tremor ( table 1 ) or on the rota - rod [ 22 , 57 ] .
in addition , as the k3 mice age , they lose substantial numbers of neurons both in the substantia nigra ( th - positive neurons ) and in the cerebellum ( basket cells ) [ 22 , 57 ] .
experimental diabetes exacerbates the tau pathology as shown for pr5 mice . as therapeutic strategies ,
different approaches can be envisaged such as the transplantation of cells with the potential to differentiate in situ either into neuronal or glial cell types .
this strategy has been successfully applied to mice with a combined tau and a pathology in which neuronal stem cell transplantation has been shown to improve cognition via brain - derived neurotrophic factor ( bdnf ) .
we used both active and passive immunization and monitored efficacy of this approach biochemically , histologically , and behaviourally .
we have also been testing a small bioavailable compound , sodium selenate that can be supplied with the drinking water .
chronic treatment over a period of four months significantly improved the pathology in both young and old k3 mice .
this was associated with reduced tau hyperphosphorylation and decreased numbers of tau inclusions including spheroids .
in fact , spheroids were completely absent from frontal cortex , suggesting that selenate reverts the functional impairment leading to spheroids .
k3 mice are characterized by a substantial , age - dependent degeneration of cerebellar basket neurons , resulting in the absence of pinceau terminals formed by clustered axons surrounding purkinje cells .
these findings show that selenate reduces tau phosphorylation and deposition , mitigates pathological spheroid formation , and prevents axonal degeneration of distinct neuronal populations in k3 mice .
we also treated 8-month - old pr5 mice with sodium selenate and found that treatment caused a significant impairment in the cta paradigm .
as for the k3 mice , selenate both caused reduced hyperphosphorylation and numbers in tau deposits ( nfts ) as well as increased tau solubility .
nft numbers in 12-month - old selenate - treated pr5 mice were similar to those in 8-month - old - untreated pr5 mice , suggesting that treatment had halted disease progression .
we further showed that the selenate - induced improvements require protein phosphatase 2a ( pp2a ) , by performing in vitro experiments and showing inefficacy of selenate in mice with a combined tau pathology and reductions in pp2a activity [ 66 , 67 ] .
while in the ad and ageing field mouse strains are available with traits that arose spontaneously and have been further established by specific breeding programs such as the senescence - accelerated sam mice , the rate at which new models arise spontaneously means that , even with a concerted effort , discovery is on an asymptomatic trajectory towards a phenotype gap .
the concept of genomewide mutagenesis is well established in invertebrates such as the fruit fly drosophila melanogaster and in the nematode worm caenorhabditis elegans , with enu mutagenesis mainly producing a to t and t to a transversions .
this mutagenesis strategy was used to create the first genetic maps of mendelian inheritance in drosophila , revealing many of the key genes controlling early embryonic patterning and development .
what makes it possible to carry out comparable efforts in mice is the extraordinary mutagenic efficiency of enu in mouse spermatogonial stem cells . by expanding the library of alleles with chemical mutagenesis using n
- ethyl - n - nitosourea ( enu ) , identification of each of the single nucleotide changes that result in a particular phenotype is feasible in mice and is now providing novel insights into not only immunity , where this approach has been used widely , but also the functioning of the brain .
genomewide , random mutagenesis with the ethylating chemical enu provides a way to produce and screen genetic variants in the mouse .
intraperitoneal injection of male founder mice ( g0 ) causes random base pair substitutions in the dna of spermatogonial stem cells .
informative mutations are identified by screening members of the pedigrees propagated from these founders for phenotypes of interest .
introducing point mutations has the advantage that it most often inactivates or alters the function of individual protein domains rather than eliminating the protein altogether ( as is the case for complete knock - outs ) , making it harder for related proteins to compensate , better mimicking the effects of drugs or natural genetic variants , illuminating active sites in proteins , and revealing specific functions of alternatively spliced forms .
we have been investigating a phenotype modulation of our k3 mice by crossing enu male founder mice with k3 females as discussed hereinafter . here , and in general ,
dominant mutations are expected to manifest in the first generation offspring ( g1 ) , while recessive mutations are brought to homozygosity in g3 mice by either g1
. the rate of recessive mutations has been established based on genotype - driven projects using libraries of g1 gametes generated from an enu - treated founder .
sequencing of sentinel regions of the genome of g1 sperm provides quantitative and qualitative characteristics of enu mutagenesis and information about the probable yield of mutations from phenotype - driven screens .
the consensus is a mutation rate of 0.51.0  mutations / mbp ( 7.5  107 
mutations / bp / g0 gamete , or 15003000  mutations / g1 ) from a standard regimen ( 100  mg / kg / week  3 for a c57bl/6 founder ) . assuming a 1600  cm 2.6 gb haploid mouse genome ,
of which approximately 1.5% is coding , this comes down to approximately 50 functional mutations in each g1 .
the efficiency of enu - induced mutation means that the generation of allelic series is highly feasible .
based on a conservative estimate of 20 point mutations per g1 gamete , a survey of 17400 g3s would yield a series of 5 functional alleles per gene with > 99% probability . limiting factors of the enu mutagenesis approach are infertility and lethality , with the standard enu regimen resulting in azoospermia ( absence of motile sperm in semen ) for up to 10 weeks , with fertility being resumed when the testes are repopulated by spermatogonia .
the presence of mutations that confer lethality before weaning sets the workable upper limit of the enu dose even more stringently .
for example , one study found that 1/13 g1 mice contained lethal mutations in the balancer chromosome 11 region that contains 5% of the genome , with the consequence that 18% of the g3 population will not survive because of lethal mutations .
for phenotype - driven strategies ( such as a motor phenotype , see the following ) , f1 and f3 mice are examined for dominant - acting and recessive - acting mutations , respectively [ 23 , 82 ] .
the recovery rate of dominant mutations is low ( 2% ) ; on the other hand however , costs for identifying recessive mutations are much higher , due to much higher mouse numbers and sample size to be screened and sequenced . in an ideal screen ,
the phenotypes are qualtitative ( such as having a motor phenotype ) or binomial ( rejection of transplanted tissue or not ) ; they are free of false positives and are sufficiently penetrant to permit meiotic mapping .
the latter is typically achieved first by outcrossing and then an f1 intercrossing to yield f2 mice ; 50 f2 mice are required to obtain a 10  mb mapping resolution with a 95% confidence , although haplotype mapping might enable an estimation of the chromosomal location using as few as 10 affected f2 mice . for a more detailed discussion of the likelihood of a confounding mutation cosegregating with the phenotype
the reader is referred to two excellent reviews on the topic [ 23 , 69 ] .
what makes the approach feasible is a simple read - out , and in this respect , motor phenotypes are much easier to monitor than memory phenotypes . as in our k3 mice ,
k369i mutant tau expression causes both a motor and memory impairment , changes to the motor phenotype by any intervention although interesting in itself can also serve as a surrogate marker for changes in memory functions .
we initiated an enu mutagenesis program in k3 mice that generated transgene - dependent and -independent pedigrees . in the following we will be discussing five pedigrees , enu164 ( partial rescue , transgene - related ) , enu67 ( slow weight gain , transgene - related ) , enu37 ( circling , not transgene - related ) , enu12/30a ( motor phenotype , not transgene - related ) , and enu16/069 ( motor phenotype , not transgene - related ) . to first establish that the k3 phenotype is fully penetrant on the background chosen for enu mutagenesis , we established backcrosses with 20 mice each , using c57bl/10 , c3h / h3h , fvb , c57led , and balb / b mice , all of which are established enu strains at the apf ( australian phenomics facility , canberra ) .
we decided to use balb / b as the strain for enu mutagenesis , as the k3 phenotype of parkinsonism is fully penetrant on this background , both with regards to phenotype and age - of - onset .
therefore , balb / b males were injected with enu and paired with the k3 mice ( that are on a c57bl/6 background ) , and the f1 offspring was analyzed .
the litters born were scored for three read - outs of parkinsonism in mice , tremor ( staging 03 ) , gait anomalies ( staging 03 ) , limb clasping ( staging 05 ) , as well as body weight ( table 1 ) .
we had previously phenotyped wild - type balb / b mice for the three parkinsonism characteristics and weight .
although none of them displayed abnormal gait or resting tremor , 37% did clasp their hind limbs when stimulated and 5% clasped their hind limbs without stimulation , while their front paws grasped the cage wire .
therefore , to ensure accurate identification of the phenotype , the hindlimb clasping alone was not used as sole indicator , but in conjunction with tremor and abnormal gait .
phenotyping was done at weaning , and then at one - week intervals until around 8 weeks of age , depending on the scoring outcome .
any mice exhibiting all three phenotypes and genotyped k3-tau positive were culled , as were any mice failing to display any of the phenotypes and genotyped k3-tau negative .
mice which showed a genotype / phenotype mismatch were genotyped a second time for confirmation and phenotyped for up to 3 months .
any mice which did not display the phenotype until after 5 weeks of age were also noted as having delayed onset of the phenotype and were flagged as a putant ( potential mutant ) of interest .
this was followed by next generation whole exome sequencing as a means to identifying mutations .
we would like to make the comment that while , previously , we have been mapping the enu mutations down to a chromosomal region for most strains these days it is much faster to directly  next generation sequence  the exomes of one to two affected mice instead .
this has greatly accelerated the speed of discovery from years to months and reduced costs of identifying causal mutations .
the snps identified with the sequencing were then validated against the phenotype with an amplifluor snp assay for each snp .
hereinafter we will be discussing five pedigrees for which we have arrived at different stages of out - crossing and identification of the underlying gene mutations , starting with those pedigrees with which we have the least progressed . 

enu164 pedigreethis pedigree is characterized by a partial rescue of the parkinsonism phenotype that characterizes the k3 mice , because some transgene - positive mice in the pedigree showed an improvement in our vigorous weekly scorings ; for example , at week 6 they would show a scoring of 1/2/1 for gait / clasp / tremor instead of 3/4/3 as is typical for k3 mice .
as mentioned above , consecutive backcrossing onto c57bl/6 reduces the number of  functional  enu - induced point mutations such that in the 3rd generation ( f3 ) they are reduced to 3 - 4 mutations . as the k3 mice have been generated on a c57bl/6 background , while enu mutagenesis has been on a balb / b inbred genetic background , strain - specific snp detection enabled the genomic mapping of the causative mutation location on the balb / b background .
this narrows down the linked mutation - carrying region to less than 20  mb , at which point candidate genes in the region will be sequenced to detect mutant alleles , and eventually , the causative mutation .
6 of these showed the inherited partial rescue phenotype ( ~15% inheritance ) . from f2 # 483
we screened 11 k3-positive mice , with 3 showing partial rescue ( ~27% ) , from f2 # 430 20 k3-positive mice ( ~35% ) .
exome ( ngs ) next generation sequencing is ongoing to identify the one single gene mutation that causes the k3 modulating phenotype . 
 
this pedigree is characterized by a partial rescue of the parkinsonism phenotype that characterizes the k3 mice , because some transgene - positive mice in the pedigree showed an improvement in our vigorous weekly scorings ; for example , at week 6 they would show a scoring of 1/2/1 for gait / clasp / tremor instead of 3/4/3 as is typical for k3 mice .
as mentioned above , consecutive backcrossing onto c57bl/6 reduces the number of  functional  enu - induced point mutations such that in the 3rd generation ( f3 ) they are reduced to 3 - 4 mutations . as the k3 mice have been generated on a c57bl/6 background , while enu mutagenesis has been on a balb / b inbred genetic background , strain - specific snp detection enabled the genomic mapping of the causative mutation location on the balb / b background .
this narrows down the linked mutation - carrying region to less than 20  mb , at which point candidate genes in the region will be sequenced to detect mutant alleles , and eventually , the causative mutation .
6 of these showed the inherited partial rescue phenotype ( ~15% inheritance ) . from f2 # 483
we screened 11 k3-positive mice , with 3 showing partial rescue ( ~27% ) , from f2 # 430 20 k3-positive mice ( ~35% ) .
exome ( ngs ) next generation sequencing is ongoing to identify the one single gene mutation that causes the k3 modulating phenotype . 

enu67 pedigreethis pedigree shows a worsening of the weight phenotype that characterises the k3 mice .
for example , while the average weight of transgene - negative mice is 19,0 grams at six weeks of age , this is reduced to 15.8 grams for transgene - positive k3 mice and is even further reduced in the enu67 pedigree ( k3-positive ) that shows an average weight of only 11.7 grams .
both k3-positive mice ( # 347 , # 350 ) were identified as slow to gain weight , and we are currently crossing further to determine the underlying gene mutation . 
 
for example , while the average weight of transgene - negative mice is 19,0 grams at six weeks of age , this is reduced to 15.8 grams for transgene - positive k3 mice and is even further reduced in the enu67 pedigree ( k3-positive ) that shows an average weight of only 11.7 grams .
both k3-positive mice ( # 347 , # 350 ) were identified as slow to gain weight , and we are currently crossing further to determine the underlying gene mutation . 

the trait shows mendelian inheritance over three generations . following deep sequencing of the exome of 2 affected mice we identified possible causal point mutations in the following genes:5730455p16rik ( riken cdna);tcof1 ( treacher collins franceschetti syndrome 1 ) , responsible for the production of treacle , which plays a role in the formation of tissue in the face .
the human disease is also autosomal dominant;tbx1 ( t - box 1 gene ) t - box genes encode transcription factors involved in the regulation of developmental processes .
chromosomal deletions in this region have been linked to neural - crest - related defects .
a point mutation could potentially cause a mild neural phenotype;senp7 ( sumo1/sentrin specific peptidase 7 gene ) involved in the posttranslational modification of proteins by the addition of ubiquitin - like sumo proteins;ggt7 ( gamma - glutamyltransferase 7 gene ) : this gene is a member of a family that encodes enzymes involved in both the metabolism of glutathione and in the transpeptidation of amino acids ; changes in the activity of   -glutamyltransferase may signal preneoplastic or toxic conditions in the liver or kidney in humans;hkdc1 ( hexokinase domain containing 1 gene ) ; chrna4 ( cholinergic receptor , nicotinic , alpha polypeptide 4 gene ) : this gene encodes a nicotinic acetylcholine receptor .
polymorphisms in this gene provide protection against nicotine addiction in humans.amplifluor primers were designed to the above snps and affected and unaffected mice genotyped , which led to the confirmation of the causal mutation in the tbx1 gene , with a t to a substitution of bp 857 ( coding position ) resulting in an asn to ile change of amino acid 276 .
a complete knock - out of tbx1 is available and is a model of digeorge syndrome ; it is further characterized by perinatal death [ 87 , 88 ] .
the digeorge syndrome is caused by a 1.5 to 3.0  mb hemizygous deletion of chromosome 22q11.2 .
haploinsufficiency of the tbx1 gene in particular is responsible for most of the physical malformations .
there is evidence that point mutations in the tbx1 gene can also cause the disorder .
digeorge syndrome is the most frequent microdeletion syndrome in humans , and is characterized by cardiovascular , thymic and parathyroid , and craniofacial anomalies .
the underlying cause is disturbed formation of the pharyngeal apparatus , a transient structure present during vertebrate development that gives rise to endocrine glands , craniofacial tissue , and the cardiac outflow tract .
the pharyngeal apparatus is composed of derivatives of ectoderm , endoderm , mesoderm , and the neural crest .
thus , complex interactions between cell types from different origins have to be orchestrated in the correct spatiotemporal manner to establish proper formation of the pharyngeal system .
the analysis of engineered mouse mutants developing a phenotype resembling digeorge syndrome has revealed genes and signalling pathways crucial for this process .
intriguingly , these mouse models reveal that interference with either of two distinct phases of pharyngeal apparatus development can contribute to the aetiology of digeorge syndrome . with regards to ad ,
furthermore , string 9.0 known and predicted protein - protein interactions for tbx1 identify sept5 which is also among 29 genes that are deregulated in pr5 tau transgenic mice ( jg and lim , manuscript submitted ) .
the value of the enu37 strain lies in the absence of a perinatal phenotype which allows for studies which are not feasible with the complete knockout . 
 
the trait shows mendelian inheritance over three generations . following deep sequencing of the exome of 2 affected mice we identified possible causal point mutations in the following genes:5730455p16rik ( riken cdna);tcof1 ( treacher collins franceschetti syndrome 1 ) , responsible for the production of treacle , which plays a role in the formation of tissue in the face .
the human disease is also autosomal dominant;tbx1 ( t - box 1 gene ) t - box genes encode transcription factors involved in the regulation of developmental processes .
chromosomal deletions in this region have been linked to neural - crest - related defects .
a point mutation could potentially cause a mild neural phenotype;senp7 ( sumo1/sentrin specific peptidase 7 gene ) involved in the posttranslational modification of proteins by the addition of ubiquitin - like sumo proteins;ggt7 ( gamma - glutamyltransferase 7 gene ) : this gene is a member of a family that encodes enzymes involved in both the metabolism of glutathione and in the transpeptidation of amino acids ; changes in the activity of   -glutamyltransferase may signal preneoplastic or toxic conditions in the liver or kidney in humans;hkdc1 ( hexokinase domain containing 1 gene ) ; chrna4 ( cholinergic receptor , nicotinic , alpha polypeptide 4 gene ) : this gene encodes a nicotinic acetylcholine receptor .
polymorphisms in this gene provide protection against nicotine addiction in humans.amplifluor primers were designed to the above snps and affected and unaffected mice genotyped , which led to the confirmation of the causal mutation in the tbx1 gene , with a t to a substitution of bp 857 ( coding position ) resulting in an asn to ile change of amino acid 276 .
a complete knock - out of tbx1 is available and is a model of digeorge syndrome ; it is further characterized by perinatal death [ 87 , 88 ] .
the digeorge syndrome is caused by a 1.5 to 3.0  mb hemizygous deletion of chromosome 22q11.2 .
haploinsufficiency of the tbx1 gene in particular is responsible for most of the physical malformations .
there is evidence that point mutations in the tbx1 gene can also cause the disorder .
digeorge syndrome is the most frequent microdeletion syndrome in humans , and is characterized by cardiovascular , thymic and parathyroid , and craniofacial anomalies .
the underlying cause is disturbed formation of the pharyngeal apparatus , a transient structure present during vertebrate development that gives rise to endocrine glands , craniofacial tissue , and the cardiac outflow tract .
the pharyngeal apparatus is composed of derivatives of ectoderm , endoderm , mesoderm , and the neural crest .
thus , complex interactions between cell types from different origins have to be orchestrated in the correct spatiotemporal manner to establish proper formation of the pharyngeal system .
the analysis of engineered mouse mutants developing a phenotype resembling digeorge syndrome has revealed genes and signalling pathways crucial for this process .
intriguingly , these mouse models reveal that interference with either of two distinct phases of pharyngeal apparatus development can contribute to the aetiology of digeorge syndrome . with regards to ad ,
furthermore , string 9.0 known and predicted protein - protein interactions for tbx1 identify sept5 which is also among 29 genes that are deregulated in pr5 tau transgenic mice ( jg and lim , manuscript submitted ) .
the value of the enu37 strain lies in the absence of a perinatal phenotype which allows for studies which are not feasible with the complete knockout .
5730455p16rik ( riken cdna ) ; tcof1 ( treacher collins franceschetti syndrome 1 ) , responsible for the production of treacle , which plays a role in the formation of tissue in the face .
the human disease is also autosomal dominant ; tbx1 ( t - box 1 gene ) t - box genes encode transcription factors involved in the regulation of developmental processes .
chromosomal deletions in this region have been linked to neural - crest - related defects .
a point mutation could potentially cause a mild neural phenotype ; senp7 ( sumo1/sentrin specific peptidase 7 gene ) involved in the posttranslational modification of proteins by the addition of ubiquitin - like sumo proteins ; ggt7 ( gamma - glutamyltransferase 7 gene ) : this gene is a member of a family that encodes enzymes involved in both the metabolism of glutathione and in the transpeptidation of amino acids ; changes in the activity of   -glutamyltransferase may signal preneoplastic or toxic conditions in the liver or kidney in humans ; hkdc1 ( hexokinase domain containing 1 gene ) ;   chrna4 ( cholinergic receptor , nicotinic , alpha polypeptide 4 gene ) : this gene encodes a nicotinic acetylcholine receptor .
enu12/301 pedigree ( in short : enu12)enu12 mice , under mild stress , show sudden jerky movements when moving around the cage and appear to tremor constantly .
some mice show what could be described as opisthotonus ( a type of spasm in which the head and heels arch backwards in extreme hyperextension and the body forms a reverse bow ) . the mice display varying degrees of severity ; even when severely affected , they are able to move freely and quickly around the cage .
they occasionally fall to either side and circle around the cage in very fast movements when highly stressed , such as when the cage is changed .
the enu12 phenotype can be recognised at weaning.deep sequencing identified an a to t substitution of bp 1408 in exon 10 of the kcnq1 gene that results in a lys to stop change of amino acid 435 .
the kcnq1 gene encodes a voltage - gated potassium channel protein required for the repolarisation phase of the cardiac action potential .
the gene product can form multimers with two other potassium channel proteins , kcne1 and kcne3 . inherited forms of a human condition known as long qt syndrome ( lqts ) ,
an abnormality in the cardiac ventricular repolarisation characterised by qt interval prolongation and abnormal t - waves on an electrocardiogram ( ecg ) , are most commonly associated with mutations in kcqn1 .
some patients show only a cardiac defect ( romano - ward syndrome , rws , dominant ) ; others may suffer from severe deafness due to a lack of endolymph in the vestibular and auditory compartment ( jervell and lange - nielsen syndrome , jlns , recessive ) .the complete knockout is a model of jnls , but the mice show an additional circling ( shaker / waltzer ) and rapid head blobbing phenotype . introducing the familial a340e point mutation by a knock - in approach models rws ( cardiac phenotype , but no inner ear defect ) .
this point mutation is in the amino - terminus of kcnq1 that contains the six transmembrane domains s1-s6 and the pore domain p .
a comparative analysis of the strains allowed the authors to draw conclusions with regards to the etiology of the cardiac phenotype .
the k434stop mutation in the enu12 mice is in the carboxy - terminus that contains four helical regions , of which two form coiled - coil assemblies .
the enu12 strain has a phenotype similar to the complete knock - out demonstrating an essential role for the helical domain in kcnq1 function . 
 
enu12 mice , under mild stress , show sudden jerky movements when moving around the cage and appear to tremor constantly .
some mice show what could be described as opisthotonus ( a type of spasm in which the head and heels arch backwards in extreme hyperextension and the body forms a reverse bow ) . the mice display varying degrees of severity ; even when severely affected , they are able to move freely and quickly around the cage .
they occasionally fall to either side and circle around the cage in very fast movements when highly stressed , such as when the cage is changed .
deep sequencing identified an a to t substitution of bp 1408 in exon 10 of the kcnq1 gene that results in a lys to stop change of amino acid 435 .
the kcnq1 gene encodes a voltage - gated potassium channel protein required for the repolarisation phase of the cardiac action potential .
the gene product can form multimers with two other potassium channel proteins , kcne1 and kcne3 . inherited forms of a human condition known as long qt syndrome ( lqts ) ,
an abnormality in the cardiac ventricular repolarisation characterised by qt interval prolongation and abnormal t - waves on an electrocardiogram ( ecg ) , are most commonly associated with mutations in kcqn1 .
some patients show only a cardiac defect ( romano - ward syndrome , rws , dominant ) ; others may suffer from severe deafness due to a lack of endolymph in the vestibular and auditory compartment ( jervell and lange - nielsen syndrome , jlns , recessive ) .
the complete knockout is a model of jnls , but the mice show an additional circling ( shaker / waltzer ) and rapid head blobbing phenotype . introducing the familial a340e point mutation by a knock - in approach models rws ( cardiac phenotype , but no inner ear defect ) .
this point mutation is in the amino - terminus of kcnq1 that contains the six transmembrane domains s1-s6 and the pore domain p .
a comparative analysis of the strains allowed the authors to draw conclusions with regards to the etiology of the cardiac phenotype .
the k434stop mutation in the enu12 mice is in the carboxy - terminus that contains four helical regions , of which two form coiled - coil assemblies .
the enu12 strain has a phenotype similar to the complete knock - out demonstrating an essential role for the helical domain in kcnq1 function . 

enu16/069 pedigree ( in short : enu16)with minimal stress , enu16 mice display a mild action tremor . with moderate stress the tremor worsens and the mice display an unusual , hypermetric gait in their hind limbs . upon restraining of the tail either one or
both hind limbs show dramatic  clasping  , and when returned to the cage , it takes some time for the mice to regain control over the limbs with what looks to be a sporadic and short - term lack of control of the hind limbs . within seconds of being
returned to the cage post restraint the gait appears to improve but can persist and be quite unsteady and  waddle - like  .
the phenotype is noticeable from 4 to 5 weeks of age.deep sequencing identified an a to g substitution of bp 530 in exon 5 of the mpz ( myelin protein zero ) gene resulting in an asp to gly change of aa 121 .
aliases of mpz are mpp , p - zero , and p0 , among others .
schwann cells , the glia of the peripheral nervous system ( pns ) , play pivotal roles in the development and maintenance of the pns .
in addition to forming the myelin sheath , schwann cells are involved in neuronal survival where they also provide support to axons during development and throughout adulthood .
the mpz gene encodes a transmembrane glycoprotein that is the major structural protein of peripheral myelin .
mutations in the mpz gene result in the autosomal dominant form of charcot - marie - tooth disease type 1 ( cmt1b ) .
this domain is important for the homotypic interactions that convey the self - adhesive properties that are essential to maintain myelin compaction and integrity .the complete p0 knockout causes a severe demyelinating neuropathy , but the function of p0 is still not fully resolved .
axons in their peripheral nerves are severely hypomyelinated , and a subset contain myelin - like figures and axons degenerate .
a second strain is available from jackson laboratories : b6.cg-mpz/grsrj mice carry the spontaneous totterer mutation .
homozygous mice are poor breeders , reproducing at most once or twice , and as females are poor mothers , use of the strain is limited .
besides the totterer strain , for p0 there is only a complete knock - out available , and not a knock - in strain .
the enu16 strain has an n121 g point mutation that is juxtaposed to the only known glycosylation site in the protein .
we found however by immunohistochemistry and western blot analysis that the mutated protein ( p0 n121 g ) is expressed at a pattern and at levels similar to wild - type .
this allows us to employ the enu16 strain in studies ( such as of adhesion ) that are not feasible with the complete knockout . 
  with minimal stress ,
the tremor worsens and the mice display an unusual , hypermetric gait in their hind limbs . upon restraining of the tail
either one or both hind limbs show dramatic  clasping  , and when returned to the cage , it takes some time for the mice to regain control over the limbs with what looks to be a sporadic and short - term lack of control of the hind limbs . within seconds of being
returned to the cage post restraint the gait appears to improve but can persist and be quite unsteady and  waddle - like  .
deep sequencing identified an a to g substitution of bp 530 in exon 5 of the mpz ( myelin protein zero ) gene resulting in an asp to gly change of aa 121 .
aliases of mpz are mpp , p - zero , and p0 , among others .
schwann cells , the glia of the peripheral nervous system ( pns ) , play pivotal roles in the development and maintenance of the pns .
in addition to forming the myelin sheath , schwann cells are involved in neuronal survival where they also provide support to axons during development and throughout adulthood .
the mpz gene encodes a transmembrane glycoprotein that is the major structural protein of peripheral myelin .
mutations in the mpz gene result in the autosomal dominant form of charcot - marie - tooth disease type 1 ( cmt1b ) .
this domain is important for the homotypic interactions that convey the self - adhesive properties that are essential to maintain myelin compaction and integrity .
the complete p0 knockout causes a severe demyelinating neuropathy , but the function of p0 is still not fully resolved .
axons in their peripheral nerves are severely hypomyelinated , and a subset contain myelin - like figures and axons degenerate .
a second strain is available from jackson laboratories : b6.cg-mpz/grsrj mice carry the spontaneous totterer mutation .
homozygous mice are poor breeders , reproducing at most once or twice , and as females are poor mothers , use of the strain is limited .
besides the totterer strain , for p0 there is only a complete knock - out available , and not a knock - in strain .
the enu16 strain has an n121 g point mutation that is juxtaposed to the only known glycosylation site in the protein .
we found however by immunohistochemistry and western blot analysis that the mutated protein ( p0 n121 g ) is expressed at a pattern and at levels similar to wild - type .
this allows us to employ the enu16 strain in studies ( such as of adhesion ) that are not feasible with the complete knockout .
this overview of pedigrees with motor phenotypes reveals that mice are amenable to enu mutagenesis and that it is possible to establish interesting phenotypes .
compared to the functional genomics approaches we have pursued in the past [ 99102 ] , enu mutagenesis is more directed as it not only identifies gene mutations that modulate an existing pathology but also has the added advantage of establishing mutant mouse strains at the same time .
as outlined above , enu tends to introduce point mutations rather than causing deletions ; this results in the expression of mutant proteins at levels that are high enough to perform functional studies .
a limitation in a more wide - spread application of enu mutagenesis though to mice is the high costs associated with establishing the mutants and identifying the underlying gene mutation and the fact that not all phenotypes are amenable to a robust and fast read - out .
therefore , using the motor phenotype as a surrogate marker for memory functions ( as for the k3 transgene - dependent pedigrees ) is an avenue that can be pursued in the field of ad mouse models .
what are the challenges of enu mutagenesis in transgenic models of neurodegeneration and what issues both practical and conceptual did we encounter while doing these studies ?
in hindsight it appears to have been the preferred strategy to start with as many enu - mutagenised males for cross - breeding with k3 mice as possible in order to saturate the genome with mutations .
also , we had initially planned to identify both dominant and recessive mutations , but considering the extensive breeding needed , we realised that such an undertaking is not affordable and therefore we concentrated solely on dominantly inherited traits . in principle
the question that may be raised is which other transgenic models could be easily screened and used for enu mutagenesis .
we believe that any strain with a phenotype that can be easily picked up is amenable to this strategy . in our case
we have been using mice that are characterized by a robust tau - dependent motor and memory phenotype , so we assumed that any modification of the motor phenotype would also modify memory functions , a trait , which would be tested after the modification of the motor phenotype has been confirmed .
another question is what the expectations are beyond finding possible new targets and how we see the field evolving in the years ahead .
we have started to establish caenorhabditis elegans in the laboratory as a system complementary to mice and it seems that overall a mutagenesis screen in the roundworm system is easier . we still believe that there is a potential in applying enu mutagenesis to mouse models . these models might nicely complement ongoing concerted efforts such as those of the international knockout mouse consortium ( http://www.knockoutmouse.org/ ) .
the main objectives of this consortium are to generate , archive , and distribute worldwide up to 12.000 conditional mutations across the mouse genome in mouse embryonic stem ( es ) cells .
the main mutagenesis strategies pursued are : ( i ) conditional gene trapping  a random approach for expressed genes ; ( ii ) conditional - targeted trapping 
a directed approach , used for expressed genes ; and ( iii ) conditional gene targeting  a directed approach , used for non - expressed genes hopefully , these concerted efforts help in developing a cure for a disease for which only symptomatic treatment is available .
 modifier screening is a powerful genetic tool . 
 while not widely used in the vertebrate system , we applied these tools to transgenic mouse strains that recapitulate key aspects of alzheimer 's disease ( ad ) , such as tau - expressing mice . 
 these are characterized by a robust pathology including both motor and memory impairment . 
 the phenotype can be modulated by enu mutagenesis , which results in novel mutant mouse strains and allows identifying the underlying gene / mutation . here 
 we discuss this strategy in detail . 
 we firstly obtained pedigrees that modify the tau - related motor phenotype , with mapping ongoing . 
 we further obtained transgene - independent motor pedigrees : ( i ) hyperactive , circling enu 37 mice with a causal mutation in the tbx1 gene  
 the complete knock - out of tbx1 models digeorge syndrome ; ( ii ) enu12/301 mice that show sudden jerky movements and tremor constantly ; they have a causal mutation in the kcnq1 gene , modelling aspects of the romano - ward and jervell and lange - nielsen syndromes ; and ( iii ) enu16/069 mice with tremor and hypermetric gait that have a causal mutation in the mpz ( myelin protein zero ) gene , modelling charcot - marie - tooth disease type 1 ( cmt1b ) . 
 together , we provide evidence for a real potential of an enu mutagenesis to dissect motor functions in wild - type and tau mutant mice . 
1. Introduction
2. Tau Transgenic Mouse Models with Memory and Motor Phenotypes
3. Modulation of Transgenic Phenotype by Therapeutic Intervention
4. ENU Mutagenesis Strategies in Mice
5. ENU Mutagenesis Applied to K3 Mice Identifies Transgene-Dependent and -Independent Pedigrees
6. Outlook