thermal spring 's microbiomes have been in attention of the scientific community since past few decades .
these springs , being hub of diverse microflora , increase the probability of vast gene pool of uncultured microbiota .
metagenomics have helped the microbiologist to reveal the genome of the rest of the 99% of non - cultivable microbes which further helped to better understand the global microbial ecology and also helped in meeting the current demand for novel enzymes .
thus , with the advances in metagenomics all the hidden facts of the microbial ecology have been faced off .
odisha being rich in bio - diversity due to seasonal / climatic variations possesses a variety of hot - springs located in different geographical locations and vary both in physio - chemical and microbial ecology parameters .
mainly four major hot - springs have been reported in odisha i.e. deulajhari hot spring in angul , taptapani hot spring in ganjam , atri hot spring in khurda and tarabalo in nayagarh .
deulajhari hot spring is located at about 6  km from athamallik and between 203 north latitude and 8449 east longitude . in our study ,
sediment sample from deulajhari hot spring ( latitude 20.74199  n , longitude 84.49206 e ) was collected from the hot spring having 69  c temperature .
the v3v4 region of the 16s rrna was amplified using primers 341f , 5-cctacgggaggcagcag-3 and 518r , 5-attaccgcggctgctgg-3 with 50  ng of metagenomic dna .
the amplified pcr product was purified by gel elution using minelute column ( qiagen , india ) and further leads to 150 nucleotide paired end multiplex sequencing using illumina gaiix sequencer at genotypic technology pvt .
krona tool was used for plotting krona graph as depicted in fig . 1 
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high quality reads obtained from the hot spring with temperature of 69  c were used for further analysis . out of the total , proteobacteria ( 88.12% ) , bacteriodetes ( 10.76% ) , firmicutes ( 0.35% ) , spirochetes ( 0.18% ) , thermi ( 0.13% ) and chloroflexi ( 0.11% ) were identified at the phylum level . of the total 216 genera in this deulajhari hot spring
only 53.7% were identified and 46.29% were unidentified at the genus level . since all the phylotypes , retrieved through the sequencing , do not contribute to all the taxonomic groups known till date , it indicates the significance of the diversity of deulajhari hot spring explored .
 insights about the distribution of the microbial community prove to be the major goal of understanding microbial ecology which remains to be fully deciphered . 
 hot springs being hub for the thermophilic microbiota attract the attention of the microbiologists . 
 deulajhari hot spring cluster is located in the angul district of odisha . covered within a wooded area , 
 deulajhari hot spring is also fed by the plant litter resulting in a relatively high amount of total organic content ( toc ) . 
 for the first time , illumina sequencing based biodiversity analysis of microbial composition is studied through amplicon metagenome sequencing of 16s rrna targeting v3v4 region using metagenomic dna from the hot spring sediment . over 28 phyla 
 were detected through the amplicon metagenome sequencing of which the most dominating phyla at the existing physiochemical parameters like ; temperature 69  c , ph  8.09 , electroconductivity 0.025  dsm  1 and total organic carbon 0.356% , were proteobacteria ( 88.12% ) , bacteriodetes ( 10.76% ) , firmicutes ( 0.35% ) , spirochetes ( 0.18% ) and chloroflexi ( 0.11% ) . 
 approximately 713 species were observed at the above physiochemical parameters . 
 the analysis of the metagenome provides the quantitative insights into microbial populations based on the sequence data in deulajhari hot spring . 
 metagenome sequence is deposited to sra database which is available at ncbi with accession no . 
 srx1459736 . 
Direct link to deposited data
Experimental design, materials and methods