heidelberger and his postdoctoral fellow forrest kendall  who had been rendered one handed ( but , by all accounts , no less dextrous ) by a farm threshing machine  took advantage of purified bacterial polysaccharides ( isolated during their earlier studies ) for their quantitative experiments .
they incubated varying proportions of purified antigen and antibody and determined the nitrogen content ( i.e. , antibody content ) of the resulting precipitate .
the data derived from these assays showed that the precipitin reaction could be expressed based on simple equations derived from the laws of mass action and that antibodies and antigens were multivalent .
when they repeated the experiment with whole serum , their calculations held up , thus assuring them that their equations were not exclusively applicable to purified solutions ( 1,2 ) .
 this was all very well for antibodies to polysaccharides ,  noted heidelberger in a 1979 article ,  but what about those elicited by the vast numbers of protein antigens ?  ( 3 ) .
as n had not yet been discovered , heidelberger and kendall instead used a colorful trick to distinguish antigen - derived nitrogen from antibody - derived nitrogen .
hen egg albumin  against which they raised specific antibodies in rabbits ( 4 ) .
the amount of antigen - derived nitrogen in the redissolved precipitate could then be determined by comparing its color ( by eye , as colorimeters did not yet exist ) with solutions containing known concentrations of the dye ; the remainder of the total nitrogen content was attributed to the antibody ( 5 ) .
heidelberger later took on his first graduate student , elvin kabat . together , they helped settle a long - standing debate concerning whether serum  precipitins  and  agglutinins  ( antibodies that agglutinate bacteria ) were the same or different . at the time , many people thought that these distinct functional properties of antibacterial antiserum could be ascribed to separate entities .
returning to the pneumococcal bacteria , the duo showed that precipitins and agglutinins were present in identical amounts in antipneumococcal serum .
reduction of one activity  by adsorbing the serum with a bacterial or polysaccharide solution  resulted in an equivalent reduction in the other activity , suggesting that the two functions were properties of the same antibody molecules ( 6 ) .
kabat went on to show that antibodies in serum came in two sizes  large and small ( now known as igm and igg)based on their mass and sedimentation rate ( 7 ) .
 heidelberger 's work ,  said former colleague herman eisner ( massachusetts institute of technology ) in a 2001 article ,  changed the concept of the antibody from an essentially ill - defined set of serum activities to a protein molecule , measurable in conventional chemical units  whose recognition of antigens could be analyzed in molecular terms 
heidelberger , who was also a talented musician and linguist , worked in the lab until his death at age 103 . at his 100th birthday party , he was reportedly asked how many papers he had published in his lifetime .  three hundred and four ,  he answered , adding slyly ,  so far 
 having defined the protein nature of antibodies under the tutelage of oswald avery , michael heidelberger was the first to apply mathematics to the reaction of antibodies and their antigens ( the  precipitin reaction  ) . 
 heidelberger 's calculations launched decades of research that helped reveal the specificity , function , and origin of antibodies . 
Obeying the law of mass action
An eye for color
Multitalented proteins