visceral leishmaniasis ( vl ) is the vector - borne disease and has great importance in public health due to the fatality among children .
although there is an increasing interest to vl but there is not enough approach for control measures .
there are various epidemiological features of vl which indicating the needs for defining different control measures ( who 1990 ) .
kala - azar is a mediterranean - form in iran which the leishmania infantum isolated from vl patients ( 

 mohebali et al .
2005 ) , the sand flies species from subgenera larroussius and paraphlebotomus acts as vectors ( 

 azizi et al .
2006 ) and the dogs and wild canids are considered as principal reservoirs ( 

 edrissian et al .
asymptomic infected dogs are suspected as the main reservoir host for sand flies to establish the transmission cycle to humans ( 

 moshfeh et al .
the confirmed endemic foci of vl are distributed in different provinces and districts of iran including : ardebil province , east azerbaijan province ( kaleibar , ahar , azarshahr districts ) , fars province ( ghir - karzin , jahrom ) semnan province , bushehr province ( poshtkooh ) , qom province , kerman province ( baft ) and alborz ( savejbolagh district ) ( 

 mohebali 2013 ) .
moreover , the sporadic cases are reported from different parts of iran annually . the isolated parasite from animal reservoir
was identified as l. infantum lon49 using biochemical assay ( isoenzyme ) in ardabil and azarbaijan provinces .
the isolated parasite is exactly the same strain which previously isolated from human cases , so it can certainly be considered that the canine family is the most important reservoirs for humans ( 

 mohebali et al .
, the interruption of the transmission cycle seems to be the most effective ( ramezani et al .
, the common control strategies between humans and carnivores vl ( zvl ) due to l. infantum is limited to early diagnosis and treatment with expensive and potentially toxic pentavalent antimonial drugs ( 

 killick - kendrick et al .
treatment of infected dogs with meglumine antimobiate ( glucantime ) may be led to decrease of sensitivity of l. infantum to these drugs ( 

 gramiccia et al .
there is no record for using of flumethrin on dogs as intervention method against control of sand flies in vl foci around the world .
continuous efforts for the control of leishmaniasis and the applicability of these studies will be needed more than ever in the new and the old world .
as surely as the vectors could be spread and transmitted the parasite from dogs to man ( 

 braga et al .
1998 ) . at the present , the methods that can prevent the dogs against visceral leishmaniasis could not be prevented spreading of infected sandflies ( 

 reithinger et al .
prophylactic soaps , shampoos and sprays with base of pyrethroids have been used on dogs with different success around the world ( 

 ashford 1989 ) . yet ,
any protective measure which could protected completely the dogs against vl , is not introduced ( 

 christopher 1996 ) . in the endemic area of the vl foci
, it is recommended that all seropositive dogs are eliminated from infected areas . in southern
europe the prevalence rate of vl is about 40 % but the elimination of dogs has not been accepted in these communities . in the most endemic areas , the health policy administration system is based on the elimination of seropositive dogs ( dietze et al .
1998 ) , but due to resource and financial limitations and failure of availability of necessary supplies are not be realized with suitable methods ( 

 david et al .
1998 ) . in europe , the chemical treatment of dogs using antimonial components have been used for dog treatment , but this method is not recommended due to drug resistant of the parasites among dogs .
the drug therapy of vl among dogs may led to temporary treatment of infected dogs ( 

 gramiccia et al .
1992 , 

 alessandro et al . 1997 ) but this drugs do not prevent recrudesce of vl .
important notice that the use of these drugs does not stop the infection of sand flies that feed on infected dogs ( 

 alvar et al .
application of residual insecticides is advisable for the control of endophagic sandflies in human dwelling , stables , poultries ( marcondes and nascimento 1995 , 

 jin et al .
2004 ) but are not applicable for the exophagic sandflies which include most of vl vectors that their resting places is outdoor ( 

 jin et al .
the present study was carried out in order to evaluate the pour - on formulation of flumethrin on owned dogs in meshkinshahr district during the activity of sandflies and effects of this method was assessed based on blood - feeding and mortality of exposed sand flies treated dogs .
totally 118 dogs of the same race and gender were selected . due to higher age ( over one year ) 3 dogs
the blood samples was prepared on filter paper in order to detect the positive cases of vl among selected dogs using direct agglutination test ( dat ) which was done in laboratory of medical parasitology , school of public health , tehran university of medical sciences , iran . before application of flumethrin ,
the parazyquantel and albendazole was administered to the dogs in order to protect the research team and it is recommended to the family to bury the dogs feces up to 48 hours in a sanitary manner .
the location of experiments in mazrae - khalaf village was selected in front of the house where the cattle stables as well as the nest of sheep dog placed adjacent to it .
the bed net size of 2  2  2 meters was installed and a sheet was spread on top of dogs for collection of knockdown sand flies .
the live sand flies were collected using suction tube from indoor and outdoor early morning as well as evening and transferred to the laboratory and fed on 5 % sucrose solution during three successive days .
the sugar pad was removed 12 hours before test . at the dusk ( 19:30 pm )
which is synchronize with activity of sand flies , first the control dogs and then the treated dogs were anesthetized with xylazine and transferred to the inside of each net .
after this stage , 150 live sand flies were released inside each bed net and the environmental condition including temperature and humidity was recorded .
subsequently the exposed sand flies were transferred to the paper caps covered with net according to physiological conditions eg blood - fed or unfed . the blood feeding condition was observed immediately after 2 hours after experiments .
before pour - on application of flumethrin , titer of the serum in dogs was determinted indicating negative . for determination of flumethrin effect on vector ,
two indicators were considered in relation to blood feeding and viability of exposed sand flies compared to control group . the blood - feeding index ( bfi ) of sand flies were 345 out of 620 ( 55.6% ) and 105 out of 614 ( 17.1% ) from 20 up to 80 days post - treatment in control and treated groups ( table 1 ) .
the bfi was varied from 56.5 to 53.5 % and from 12.3 to 25 % respectively in control and treatment groups ( fig .
the difference between bfi in control and treated groups was significant ( p < 0.0001 ) .
blood - feeding index ( bfi ) of sand flies after application of pour on flumethrin on dogs using bed net trap , meshkinshahr , ardabil province , 2013 blood - feeding index ( bfi ) of sand flies after application of pour on flumethrin in dogs using bed net traps , meshkinshahr , ardabil province , 2013 application of pour - on flumethrin during the peak of blood - feeding activity of sand flies were assessed at four interval times post - treatment ( fig .
the ibf of sand flies on treated dogs was 75.0 to 87.7 % and on untreated dogs was 46.541.3 % respectively .
the ibf of sand flies was stasistically differenent between treated and control dogs ( p < 0.0001 ) . inhibition of blood - feeding rate ( ibf ) of sand flies after application of pour - on flumethrin on dogs , in bed net trap , meshkinshahr , ardabil province , 2013 the mortality rate of unfed and blood - fed sand flies was studied .
the total number of dead blood - fed sand flies was 115 out of 620 in treated dogs and 100 out of 614 respectively in control and treated groups .
the variation of mortality rate in the dead blood - fed sandflies during four interval times was 2532 % and 1538 % respectively in control and treated groups ( table 2 , fig .
mortality rate of blood - fed and unfed sand flies after application pour - on flumethrin on dogs compared to control group using bed net traps , meshkinshahr , ardabil province , 2013 mortality rate of sand flies after application pour - on flumethrin on dogs using bed net traps , meshkinshahr , ardabil province , 2013 the mortality rate of dead unfed sand flies was varied from 5 to 51 % and 94.8 to 100 % respectively in control and treated groups .
the difference in total mortality rate in control and treated sand flies was significant ( p < 0.001 ) .
the combined effect of inhibition of blood feeding and mortality rate of sand flies exposed to treated dogs compared with control group .
the combined effect was varied between 94.8 to 100 % and 19.5 to 53.3 % respectively in control and treated groups .
the difference of combined effect between four interval times of observation was not significant either treated neither group nor control except for 20 days in control group ( p < 0.001 ) .
before pour - on application of flumethrin , titer of the serum in dogs was determinted indicating negative . for determination of flumethrin effect on vector ,
two indicators were considered in relation to blood feeding and viability of exposed sand flies compared to control group .
the blood - feeding index ( bfi ) of sand flies were 345 out of 620 ( 55.6% ) and 105 out of 614 ( 17.1% ) from 20 up to 80 days post - treatment in control and treated groups ( table 1 ) .
the bfi was varied from 56.5 to 53.5 % and from 12.3 to 25 % respectively in control and treatment groups ( fig .
the difference between bfi in control and treated groups was significant ( p < 0.0001 ) .
blood - feeding index ( bfi ) of sand flies after application of pour on flumethrin on dogs using bed net trap , meshkinshahr , ardabil province , 2013 blood - feeding index ( bfi ) of sand flies after application of pour on flumethrin in dogs using bed net traps , meshkinshahr , ardabil province , 2013
application of pour - on flumethrin during the peak of blood - feeding activity of sand flies were assessed at four interval times post - treatment ( fig .
the ibf of sand flies on treated dogs was 75.0 to 87.7 % and on untreated dogs was 46.541.3 % respectively .
the ibf of sand flies was stasistically differenent between treated and control dogs ( p < 0.0001 ) . inhibition of blood - feeding rate ( ibf ) of sand flies after application of pour - on flumethrin on dogs , in bed net trap , meshkinshahr , ardabil province , 2013
the total number of dead blood - fed sand flies was 115 out of 620 in treated dogs and 100 out of 614 respectively in control and treated groups .
the variation of mortality rate in the dead blood - fed sandflies during four interval times was 2532 % and 1538 % respectively in control and treated groups ( table 2 , fig .
mortality rate of blood - fed and unfed sand flies after application pour - on flumethrin on dogs compared to control group using bed net traps , meshkinshahr , ardabil province , 2013 mortality rate of sand flies after application pour - on flumethrin on dogs using bed net traps , meshkinshahr , ardabil province , 2013 the mortality rate of dead unfed sand flies was varied from 5 to 51 % and 94.8 to 100 % respectively in control and treated groups .
the difference in total mortality rate in control and treated sand flies was significant ( p < 0.001 ) .
the combined effect of inhibition of blood feeding and mortality rate of sand flies exposed to treated dogs compared with control group .
the combined effect was varied between 94.8 to 100 % and 19.5 to 53.3 % respectively in control and treated groups .
the difference of combined effect between four interval times of observation was not significant either treated neither group nor control except for 20 days in control group ( p < 0.001 ) .
major are the responsible for the transmission of the disease ( 

 nadim et al .
1997 , 

 2000 , 

 2005 , 

 2012 , 

 azizi et al .
according to the census of iranian center for control of disease , during the 1988 to 2005 , the total number of recorded cases of vl in the country was 2056 cases which 624 of them ( 30% ) related to ardabil province ( 

 mohebali 2013 ) . in a seroepidomiological study to determine the prevalence of vl between the years of 2002 to 2005 , the more positive cases related to the northwest , especially ardabil province ( 

 mohebali et al .
, the control approach of vl due to l. infantum is reserved to early detection and treatment of the patients with expensive and potentially toxic of pentavalent antimonial drugs . in some countries , where the disease is endemic situation ,
the main control strategies has been established for indoor residual spraying as well as reservoir population control ( infected dog culling ) . in brazil , in spite of residual spraying of indoor places and annually elimination of the dogs , the trend of zvl has been increased over the past 20 years ( 

 marcelino et al .
methods for the elimination of dogs was questionable due to availability of facilities as well as social acceptance ( 

 reithinger et al .
treatment of leishmania - infected dogs with anti - leishmania drugs was not effective due to recrudescence of vl ( up to 74.0% ) ( 

 mohebali et al . 2002 ) .
while waiting for a vaccine against leishmania , the new ways switch to interrupt the vl transmission , by passing the dogs from livestock deltamethrin impregnated bath ( 

 david et al .
1994 , 

 guanghua et al . 1994 ) as well as topical application of pyrethroid lotions .
epidemiological effects of topical application of insecticide on dogs , depends on not only reducing the number of sand flies blood - fed on dog , but also survival rate of sand flies infected with leishmania ( 

 reithinger et al .
the main way for the control of vl is protection human and dosmetic reservoir from bite of sand flies ( 

 poli et al .
1997 ) . despite the nature of vl epidemiology which the special species of sand flies are responsible for transmission of the disease from infected dogs to others including human . nevertheless due to specific behavior of the vectors , control of vl
different methods including vaccinations , treatment of dogs , separation of infected dogs has been used with different results in the vl foci around the world ( 

 ashford et al .
in the recent decade , the application of deltamethrin impregnated collars has been successfully used with high social acceptance as prevention measure for the dogs in different countries .
the latter method has the repellency and killing effects against sand flies and so protected the dogs against vl ) ramezani et al .
the feeding rate of sand flies on treated dogs was 3.3 folds lower than control .
the inhibition of feeding rate on treated dogs was 1.8 fold higher than controls respectively .
the difference between total mortality of treated and control groups was highly significant ( p= 0.0001 ) . in this study ,
the immediate mortality rate of sand flies was 5 fold more than control group during 2 hours exposure time .
the delayed mortality rate of sand flies was known as a best indicator for the evaluation .
the delayed mortality rate in blood - fed sand flies was 94.8 % compared to 27.5 % in control group .
it seems the age of dogs is an important factor for pour - on application of flumethrin .
it also appears that inhibition of blood feeding or combined effects of flumethrin is prior to killing effect . according to the results
, this method could be a good alternative for control of sand flies of vl with high social acceptance by dog owners .
 background : visceral leishmaniasis ( vl ) is one of the most important parasitic zoonotic diseases in the world . 
 domestic dogs are the main domestic reservoirs of vl in endemic foci of iran . 
 various methods , including vaccination , treatment of dogs , detection and removal of infected dogs have different results around the world . general policy on control of canine visceral leishmaniasis is protection of them from sand fly bites . 
 the aim of this study was evaluation of pour - on application of flumethrin on dogs against blood - feeding and mortality of field - caught sand flies.methods:once every 20 days from may untill september 2013 , the treated and control dogs were exposed with field caught sandflies for 2 hours under bed net traps . after the exposure time , both alive and dead sand flies were transferred in netted cups to the laboratory . 
 the mortality rate of them was assessed after 24 hours . 
 the blood - fed or unfed conditions were determined 2 hours after exposure to the dogs under stereomicroscope.results:the blood feeding index was varied from 12.0 to 25.0 % and 53.0 to 58.0 % for treated and control dogs respectively ( p < 0.0001 ) . 
 the blood feeding inhibition was 75.087.0 % and 41.046.0 % for the control and treated dogs ( p < 
 0.0001 ) , respectively.the total mortality rate was 94.0100 % and 19.058.0 % respectively for the treated and control groups ( p < 0.001).conclustion : application of pour - on flumethrin on dogs caused 90100 % mortality until 2.5 month and inhibited the blood - feeding of sand flies . 
Introduction
Materials and Methods
Results
1- Direct Agglutination Test (DAT):
2- Blood feeding index of sandflies:
3- The effects of application of pour-on flumethrin on inhibition of blood-feeding (IBF) of sand flies:
4- Estimation of mortality rate:
5- Estimation of the combined effect of flumethrin pour-on:
Discussion
Conclusion