, early evidence characterizing spontaneous physical activity in children has shown that children take frequent ( 83 to 89 bouts per hour ) , short - duration ( mean duration of 20 to 21  s ) bouts of movement of variable but mostly low intensity , interspersed by comparatively long intervals of nonactive time [ 2 , 3 ] .  
this pattern of movement was termed the  tempo  of physical activity , that is , the frequency with which an activity event occurs and the intervals between these .
it has been suggested that these pattern characteristics may be physiologically more important than composite measures of physical activity .
for instance , dynamic heart rate recovery times   were noted to be of similar length to the nonactive intervals between bouts of movement .  
more recent evidence has shown that bouts of movement were shorter and less intense in overweight compared to lean boys .  
evidence from spontaneous walking in adults has also shown that the duration of each walking bout resulted in obese adults walking about 2  hrs per day less than the lean , equivalent to approximately 3.5 fewer miles walked per day .   in the same study
, participants were overfed for a period of 8 weeks resulting in weight gain and a decrease in the distance walked per day in the obese , suggestive of a mechanistic link between obesity , weight control , and spontaneous physical activity .   whereas various social , psychological , and environmental factors have been shown to explain interindividual differences in childhood physical activity levels , intra - individual variation in free - living movement enables scrutiny of the extent to
available data suggests that total physical activity shows low intra - individual variation , with values between 20 and 25% in both children and adults [ 9 , 10 ] .  
similarly , a lack of variation in daily physical activity in response to changes in the physical environment in children has been used to support the argument that there is a physical activity phenotype .   what is not known is the degree to which the tempo of spontaneous physical activity varies within a child and how the tempo of physical activity differs with weight status .  
the primary objective of this study , therefore , was to provide information on the pattern characteristics of physical activity in lean and obese girls and boys during the school day and over the weekend and the intra - individual variation in these .
specifically , we hypothesized that ( i ) lean children would have more frequent and intense movement bouts and shorter intervals between these in comparison to the obese , independent of sex and location ( school versus home ) ; ( ii ) intra - individual variability in the pattern characteristics of physical activity would be similar in the lean and obese , independent of sex and location . to achieve this , we utilized cluster recognition algorithms to characterize the tempo of physical activity from second - by - second triaxial accelerometer output .
the algorithms are based on the work of veldhuis and johnson , which were originally used to characterize the pulsatile patterns of hormone release but have since been used to identify activity pattern characteristics .
chinese children aged 7 to 9 years were recruited from three government primary schools in hong kong . from a potential of 600 7- to 9-year olds , 180 volunteered to participate .  
height and weight were assessed in school in all 180 pupils , and those above the 90th percentile for age- and sex - specific bmi cutoffs , as well as a group of age- and sex - matched lean children , were invited to join the study .
eighty - four children ( 42 lean and 42 obese , 50% male ) agreed to have physical activity assessed for three weeks .
the experimental protocols were approved by the institutional review board for human ethics and written parental consent was obtained for all children . following recruitment , the children met with research staff in the early morning at school prior to the start of lessons .
baseline anthropometric measurements were taken on the first day , and the children were instructed as to the correct care and usage of the accelerometer .
all children were asked to wear the accelerometer for three weeks with 9 of the possible 15 weekdays and 3 of the possible 6 weekend days randomly chosen for each child and used for the analyses .
body mass was determined barefoot and in light clothing with an accuracy of 0.1  kg using digital scales ( tanita tbf-401 , japan ) .
height was measured with an accuracy of 0.1  cm on a fixed stadiometer ( invicta 2007246 , leicester , uk ) .
waist circumference was measured at the narrowest point between the bottom of the ribcage and the top of the iliac crest and taken at the end of expiration .
hip circumference was measured at the level of the greater trochanters , where the buttocks protruded most .
each circumference was taken twice and recorded to the nearest 0.1  cm . a triaxial accelerometer ( rt3 , stayhealthy inc .
this small , light piezoelectric device is designed to detect accelerations in three axes and combine these to provide a triaxial vector magnitude count .
the rt3 has been shown to be a valid and reliable device for assessing physical activity in caucasian and chinese children [ 1517 ] .
intermonitor variability , however , does exist , and in an attempt to counter this we ensured that each child used the same device throughout the measurement period ( changed only if the device was not working satisfactorily ) .  
additionally , before commencement and at the finish of each measurement day , every rt3 device was checked to determine the deviation in activity counts for each axis .
devices were passed if all axes were kept within 5 counts , which ensured minimal intermonitor variability .
the study began with 40 rt3 devices , and four failed the intermonitor variability check during the course of the study , leaving 36 devices by the end of the study .
after the intermonitor variability check , devices were initialized according to manufacturer specifications and set in the vector magnitude , 1-s epoch mode .
the rt3 was placed in a close - fitting bag , attached to a belt , and fastened firmly on the right hip , immediately superior to the iliac crest on the midaxilla line .  
the children were asked to wear the device at school from 7.30  am until they returned home , which was usually 4.30  pm . on the weekend , they were asked to wear the device from the moment they got out of bed until when they went to bed in the evening . a daily monitoring period of 9 hours
was delineated by the memory capacity of the accelerometer . to try to control   wear time , we considered periods of consecutive zeros exceeding 60 minutes as nonwear time .
four children recorded a day with more than 60 minutes of consecutive zeros , and when asked about these periods they all reported taking the monitor off .
this method involves using a series of algorithms to search for significant increases and decreases within the data series . these peaks and troughs
identification and classification is based upon the threshold values which we have previously published , where nonactive behavior is defined as < 7.0  rt3vmag  s ; low - intensity activity is defined as 7.0 to < 31  rt3vmag  s , moderate intensity as 31 to < 68.5  rt3vmag  s , and vigorous intensity activity is defined as 68.5  rt3vmag  s. a bout was , therefore , defined as a continuous segment whose element value was above h , the preset nonactive threshold 7.0  rt3vmag  s. the duration of each bout was denoted as td , and the duration of the nonactive interval between two adjacent bouts was denoted as ti ; the maximum element value or peak amplitude of an activity bout was denoted as vp .
the variables computed by the program include the total number of bouts per day , mean duration of daily bouts in seconds ( s ) , mean duration of the rest intervals between bouts in seconds ( s ) , and the mean peak amplitude of daily bouts ( rt3vmag  s ) .
we also report the total minutes spent sedentary , in low , moderate , and vigorous activity for lean and obese .
baseline anthropometric data were compared between the lean and obese using one - way analyses of variance .
factorial analyses of variance with repeated measures were used to determine the main effects of weight status ( lean and obese ) , sex and location ( school versus home ) on activity pattern characteristics , and the variance in these activity pattern characteristics ( expressed as the coefficient of variation ) .
eighty - four children ( 42 lean and 42 obese ) completed the anthropometric assessment .
there was no significant difference in age , although the obese were taller and heavier and had a greater waist and hip circumference compared to the lean .
physical activity pattern characteristics derived from the bout identification algorithms are presented in table 2 .  
all 84 children completed the school - day monitoring , whilst 40 lean and 39 obese children completed the weekend monitoring .
factorial anovas revealed significant main effects for location ( school versus home ) for the number of activity bouts ( f(1,75 ) = 79.58 ; p < .001 ,  0.515 ) , the mean length of activity bouts ( f(1,75 ) = 9.93 ; p < .01 ,  0.117 ) , and the rest interval between bouts ( f(1,75 ) = 21.76 ; p < .001 ,  0.225 ) .
followup analyses indicated that the number and length of activity bouts were greater at school than at home , whilst the rest intervals were longer at home compared to school ( see table 2 ) .
a significant main effect for weight status was apparent for the number of activity bouts per day ( f(1,75 ) = 38.48 ; p < .001 ,  0.275 ) , with the lean engaging in more bouts of activity during school and at home in comparison to the obese ( p < .05 ) .  
a significant main effect for weight status was also apparent for the duration of rest intervals between bouts ( f(1,75 ) = 11.74 ; p < .01 ,  0.135 ) , with the obese taking longer rest intervals than the lean both at school and at home ( p < .05 )
.   there were no significant location by sex interactions for any variable , or weight status by sex , but there was a significant location by weight status interaction for the duration of the rest intervals between bouts ( f(1,75 ) = 8.12 ; p < .05 ,  0.098 ) .  
followup analyses showed that between - bout intervals were significantly longer in the obese and lean at home compared to school , but the difference between home and school was much more pronounced in the obese ( see table 2 ; p < .05 ) .
the mean minutes per day spent sedentary or in low , moderate , or vigorous physical activity are provided in table 3 .
there was no main effects for weight status , sex , or location for the total minutes spent sedentary , engaged in low , moderate , or vigorous activity ( p > .05 ) .  
the intra - individual variation in day - to - day activity pattern characteristics are shown in table 4 .
the factorial anova revealed significant main effects for location for the variance in the number of activity bouts per day ( f(1.71 ) = 55.9 ; p < .001 ,  0.441 ) , the duration of bouts ( f(1.71 ) = 25.3 ; p <
.001 ,  0.263 ) , interval between bouts of activity ( f(1.71 ) = 82.9 ; p < .001 ,  0.539 ) , and bout intensity ( f(1.71 ) = 13.5 ; p < .001 ,  0.159 ) .
followup analyses showed that variance increased at home compared to the school regardless of sex ( p < .05 ) .
the only interaction was for bout peak intensity , where a significant location by sex interaction was present ( f(1,71 ) = 7.70 ; p < .01 ,  0.098 ) .
followup analyses showed that intra - individual variance in the peak intensity of activity bouts was constant in boys whether at school or at home ( p > .05 ) .  
in girls , however , variance in the peak intensity of activity bouts increased significantly at home in comparison to school ( p < .05 ) . a significant main effect for sex in intra - individual variance existed for the mean duration of an activity bout ( f(1,71 ) = 3.97 ; p < .05 ,  0.053 ) , where variance was greater in the girls ( p < .05 ) .   similarly , significant main effect for sex in intra - individual variance in the peak intensity of an activity bout was apparent ( f(1,71 ) = 14.22 ; p < .001 ,  0.167 ) , with variance greater in the girls when at home ( p < .05 ) .   
significant main effects for weight status were apparent in the intra - individual variance in the number of bouts per day ( f(1.71 ) = 5.00 ; p < .05 ,  0.066 ) and the rest interval between these activity bouts ( f(1,71 ) = 6.1 ; p < .05 ,  0.079 ) . in both cases , variance was higher in the obese than the lean ( p < .05 ) .
free - living movement in children has been shown to comprise combinations of movements of varying intensities that together form bouts of activity .
these bouts have previously been found to be transient and largely composed of low - intensity walking .
the activity pattern characteristics that we report for the school day are remarkably similar to the previous observation studies [ 2 , 3 ] in terms of the number of bouts per hour , the duration of the rest interval between bouts , and the intensity of bouts .
the average physical activity bout length in our study of between 10 and 17  s corresponds well with bailey et al .
's finding that 95% of children 's physical activity bouts last less than 15  s .   previous work using heart rate monitoring
has already confirmed that children rarely sustain 10- or 20-minute bouts of moderate - intensity activity [ 1921 ] .
the data from our study provide further evidence that children 's free - living movement patterns are comprised of many , low - intensity , very short - duration bouts .
in contrast to the work of stone et al . , we found that the length and intensity of activity bouts were similar in the lean and obese children , and these showed low intra - individual variation , regardless of location .
we found that the intra - individual variance in activity pattern characteristics during school days were generally modest , varying from 9 to 15% in the lean and 8 and 26% in the obese .
these are not dissimilar from previous findings of intra - individual variability in composite markers of physical activity , which are reported to be between 20 and 25% in children .   
less frequent movement bouts , coupled with longer rest periods , distinguished the obese from the lean .
it is noteworthy that the length of time spent resting between bouts of movement shows the greatest intra - individual variation . at the weekend
, the frequency of activity bouts declined in both the lean and obese , girls and boys alike .  
similarly , the rest interval between movement bouts increased over the weekend in both the lean and obese , but this increase was substantially greater in the obese .
this is the first study , to our knowledge , that has reported the variance in the duration of sedentary intervals between bouts of activity in lean and obese children , and our findings suggest that the amount of time spent resting between movement bouts appears to be a key characteristic differentiating lean from obese children .
obese adults have been found to sit for about 2 hours more than the lean , and our findings suggest that a similar , albeit less pronounced , difference may exist in lean and obese children .
the cross - sectional design of the present study limits the ability to fully understand the relationship between rest intervals and weight status in the young , and experimental or longitudinal work is recommended .  
the likelihood of increasing aspects of physical activity that are uncommon in daily movement patterns , such as longer duration bouts of more intense physical activity , is probably poor .
similarly , aspects of physical activity that vary little within an individual are less likely to respond to intervention .  
attention may be better focused upon decreasing the periods of rest between bouts of movement and increasing the number of short - duration bouts of movement per day .
the primary limitation of this study is the relatively small sample which may increase the chance of type ii errors .
the data are also restricted to 79-year - old hong kong chinese children , and this may limit the generalisability of the findings . it is notable that our primary findings are similar to those of bailey et al .
; however , direct comparisons of our findings to other data sets are challenging because data collection approaches are not uniform .
the advent of more sophisticated and affordable monitoring tools , such as triaxial accelerometers with high sampling rates and large memory capacity , will certainly improve this situation and should encourage greater harmonization of accelerometry methodology .
we choose not to assess the physical activity patterns at home during the weekday because we felt hong kong children were less likely to be active at this time given the homework that is completed during the week ; however , these data may provide valuable information about the interplay between movement bouts and inactivity during periods of likely high sedentary behavior and should be considered in future studies .
to conclude , this study has shown that , similar to previous findings , chinese children engage in many predominantly low - intensity bouts of short - duration activity .
small lifestyle changes are quite likely to have considerable impact on activity pattern characteristics and identifying the biological and environmental ( both physical and sociocultural ) antecedents of this pattern of movement is an important next step .
the obese child engages in fewer activity bouts and takes longer rest intervals between bouts , both during the school day and at home . as the prevalence of obesity continues to rise
, efforts should also be directed toward understanding the consequences of extended rest periods between bouts of movement and fewer movement bouts a day , as well as finding ways to reverse these .
 physical activity and sedentary behavior are central components of lifetime weight control ; however , our understanding of dimensions of these behaviors in childhood is limited . 
 this study investigated free - living activity pattern characteristics and the individual variability of these characteristics in 84 lean and obese chinese children ( 79  y ) during the school day and over the weekend . 
 activity pattern characteristics were established from triaxial accelerometry ( stayhealthy rt3 ) . results indicated that children 's free - living activity is characterized by many short - duration , low - intensity bouts of movement . 
 obese children take longer rest intervals between bouts and engage in fewer activity bouts both at school and at home .   
 intraindividual variability in activity patterns was low during school days but high for the rest intervals between bouts and number of activity bouts per day at the weekend . finding ways to reduce the rest time between bouts of movement and increase the number of movement bouts a child experiences each day is an important next step . 
1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusion