our initial sample consisted of 87 hcv+ participants who were recruited from several hepatology clinics and infectious disease clinics located throughout the greater los angeles catchment area .
controls ( n = 21 ) were recruited from the community through advertisements and flyers . all procedures received prior approval by the university of california , los angeles and va greater los angeles healthcare system institutional review board committees for studies involving human subjects .
all hcv+ patients participating in this study met clinical criteria for initiating hcv therapy but had not yet begun treatment .
all data reported in the current study were collected at participants ' baseline visit . as part of procedures outlined in the parent study ,
there were no statistically significant differences between hcv+ participants who underwent neuroimaging and those who did not on key demographic variables such as age , sex , ethnicity , past drug abuse , current drug use , model for end - stage liver disease ( meld ) score , and psychiatric functioning ( all p values > 0.05 ) .
inclusion criteria were as follows : ( 1 ) 18 years of age , ( 2 ) able to read and write in english , ( 3 ) and completed at least 6th grade education .
exclusion criteria were as follows : ( 1 ) decompensated cirrhosis / liver failure ( detected by blood tests or liver biopsy ) with meld scores > 12 ; ( 2 ) current or past psychotic spectrum disorder ; ( 3 ) significant depression as judged by the study psychiatrists / psychologists ( defined as current moderate or severe major depressive disorder ) or suicidal ideation ( factors that were controlled for initiating interferon treatment ) ; ( 4 ) history of learning disability , seizure disorder , closed - head injury with loss of consciousness in excess of 30 minutes , or any other neurologic disease ; ( 5 ) evidence of any other cns opportunistic infection or neoplasm ; ( 6 ) hepatitis b infection ; ( 7 ) diagnosis of hiv infection as evidenced by hiv antibody testing ; ( 8) recent illicit drug use ( confirmed by urine toxicology ) ; and ( 9 ) contraindications for mri ( for the nested cohort who underwent neuroimaging ) . after applying exclusion criteria , our final sample ( those eligible for analysis ) included 76 hcv+ participants and 20 controls .
ten hcv+ participants were determined to be ineligible due to liver cirrhosis , and 1 control participant tested positive for stimulants at study visit .
brain mri was performed using a 3-tesla trio mri scanner ( siemens medical system , erlangen , germany ) .
high - resolution mri included t1-weighted images using a magnetization - prepared rapid acquisition gradient - echo sequence using the following parameters : repetition time ( tr)/echo time ( te ) = 2,220/2.2 msec , inversion time = 900 msec , average = 1 , matrix size = 256  256 , field of view ( fov ) = 240  240 mm , slice thickness = 1 mm , number of slices = 176 .
dti was acquired using a single - shot echo planar dual spin echo sequence with ramp sampling .
the b - factor was set to 1,000 s / mm , tr = 9,600 msec , te = 90 msec , flip angle = 90 , and averages = 1 .
a total of 71 axial sections were acquired using an image matrix of 130  130 , a slice thickness of 2 mm with no interslice gap , and an fov of 256  256 mm .
proton spectra were collected using the 2d proton magnetic resonance spectroscopy imaging ( mrsi ) technique with the volume of interest ( voi ) preselected by means of point - resolved spectroscopy ( press ) .
volume - selective 2d mrsi was performed on a 20-mm slab superior to the ventricles with te of 30 msec and tr of 2,000 msec .
the nominal voxel size was 2.82 cm ( 16  16 phase encode steps over an 18  20 cm fov ) .
outer - volume saturation bands were applied to all 6 sides of the voi localized by the press sequence to suppress the lipid contamination .
we assessed attention using the wechsler adult intelligence scale - third edition ( wais - iii ) letter - number sequencing subtest and the paced auditory serial addition test ( only the first 50 trials ) ; processing speed using the wais - iii digit symbol and symbol search subtests , trail making test part a , and stroop color naming and word reading ; learning and memory using the hopkins verbal learning test - revised and the brief visuospatial memory test - revised ; verbal / language fluency using the controlled oral word association test , executive functioning using trail making test part b and stroop color - word interference test ; and motor speed using the grooved pegboard test .
raw scores were converted into demographically adjusted t scores , grouped by neurocognitive domain , and averaged to create domain t scores .
the vas contains 18 visual analogue lines with bipolar anchors with descriptors relating to energy and fatigue .
internal consistency of the vas in our sample was high ( cronbach  = 0.86 ) .
the total score from the vas - fatigue subscale was examined in the current study .
we used roieditor for preprocessing procedures according to a modified version of the active contour method and diffeomap .
white matter parcellation map was used to segment the brain into 130 regions based on anatomical labeling , which included both the gray and white matter .
once these regions were extracted , the cortex and the surrounding white matter were segmented using an fa threshold of 0.25 .
we focused our analyses on regions of the corona radiata , insula , internal capsule , external capsule , striatum , fronto - occiptial fasciculus , cingulum , thalamus , hippocampus , and amygdala based on prior studies .
for mrsi , metabolites were quantitated in each voxel using the frequency - domain fitting routine called lc - model algorithm , which analyzes the in vivo brain spectrum as a linear combination of individual simulated metabolite spectra that constitute the basis set or prior knowledge .
we were able to quantify total naa_naag ( naa + naa glutamate ) , total choline ( gpc_pch ) , mi , and glutamate / glutamine ( glu_gln ) for regions of frontal white and gray matter , parietal white and gray matter , and basal ganglia ( see figure 2 ) .
the accuracy of the quantitation was characterized using cramer - rao lower bound ( crlb ) , and metabolite ratios with crlb < 30% were considered for further analysis .
no statistically significant differences were found for potential confounding variables such as age , years of education , ethnicity , and estimated premorbid functioning ( as measured by the wechsler test of adult reading ) ( all p values > 0.05 ) .
groups also did not differ in current drug use . between - groups analysis of variance ( anova )
was used to examine differences between hcv and control groups on our 31 outcome variables of interest : neuroimaging ( 23 variables ) , neurocognitive assessment ( 7 variables ) , and fatigue ( 1 variable ) .
outcome variables were all analyzed on a continuous scale and assumptions for performing anova and pearson correlations were checked prior to running analyses .
demographic and drug use characteristics of the sample we used the false discovery rate ( fdr ) to correct for multiple comparisons with our outcome measures .
fdr is sometimes preferred over family - wise error rate methods ( e.g. , bonferroni ) because it provides greater statistical power and reduces the chance of making type ii errors .
given our available sample size of 96 for the neurocognitive and fatigue data , adjusted  level of 0.01 ( for multiple comparisons ) , and power of 0.80 , we had enough power to detect medium effects ( f = 0.31 ) .
this is consistent with effect sizes reported in previous investigations that have examined neurocognitive differences between hcv+ patients and controls . for neuroimaging analyses , with a sample of 49
this is also consistent with the effect sizes reported by neuroimaging studies that have compared hcv+ patients to controls .
all procedures received prior approval by the university of california , los angeles and va greater los angeles healthcare system institutional review board committees for studies involving human subjects .
all hcv+ patients participating in this study met clinical criteria for initiating hcv therapy but had not yet begun treatment .
all data reported in the current study were collected at participants ' baseline visit . as part of procedures outlined in the parent study ,
there were no statistically significant differences between hcv+ participants who underwent neuroimaging and those who did not on key demographic variables such as age , sex , ethnicity , past drug abuse , current drug use , model for end - stage liver disease ( meld ) score , and psychiatric functioning ( all p values > 0.05 ) .
inclusion criteria were as follows : ( 1 ) 18 years of age , ( 2 ) able to read and write in english , ( 3 ) and completed at least 6th grade education .
exclusion criteria were as follows : ( 1 ) decompensated cirrhosis / liver failure ( detected by blood tests or liver biopsy ) with meld scores > 12 ; ( 2 ) current or past psychotic spectrum disorder ; ( 3 ) significant depression as judged by the study psychiatrists / psychologists ( defined as current moderate or severe major depressive disorder ) or suicidal ideation ( factors that were controlled for initiating interferon treatment ) ; ( 4 ) history of learning disability , seizure disorder , closed - head injury with loss of consciousness in excess of 30 minutes , or any other neurologic disease ; ( 5 ) evidence of any other cns opportunistic infection or neoplasm ; ( 6 ) hepatitis b infection ; ( 7 ) diagnosis of hiv infection as evidenced by hiv antibody testing ; ( 8) recent illicit drug use ( confirmed by urine toxicology ) ; and ( 9 ) contraindications for mri ( for the nested cohort who underwent neuroimaging ) .
after applying exclusion criteria , our final sample ( those eligible for analysis ) included 76 hcv+ participants and 20 controls .
ten hcv+ participants were determined to be ineligible due to liver cirrhosis , and 1 control participant tested positive for stimulants at study visit .
brain mri was performed using a 3-tesla trio mri scanner ( siemens medical system , erlangen , germany ) .
high - resolution mri included t1-weighted images using a magnetization - prepared rapid acquisition gradient - echo sequence using the following parameters : repetition time ( tr)/echo time ( te ) = 2,220/2.2 msec , inversion time = 900 msec , average = 1 , matrix size = 256  256 , field of view ( fov ) = 240  240 mm , slice thickness = 1 mm , number of slices = 176 .
dti was acquired using a single - shot echo planar dual spin echo sequence with ramp sampling .
the b - factor was set to 1,000 s / mm , tr = 9,600 msec , te = 90 msec , flip angle = 90 , and averages = 1 .
a total of 71 axial sections were acquired using an image matrix of 130  130 , a slice thickness of 2 mm with no interslice gap , and an fov of 256  256 mm .
proton spectra were collected using the 2d proton magnetic resonance spectroscopy imaging ( mrsi ) technique with the volume of interest ( voi ) preselected by means of point - resolved spectroscopy ( press ) .
volume - selective 2d mrsi was performed on a 20-mm slab superior to the ventricles with te of 30 msec and tr of 2,000 msec .
the nominal voxel size was 2.82 cm ( 16  16 phase encode steps over an 18  20 cm fov ) .
outer - volume saturation bands were applied to all 6 sides of the voi localized by the press sequence to suppress the lipid contamination .
we assessed attention using the wechsler adult intelligence scale - third edition ( wais - iii ) letter - number sequencing subtest and the paced auditory serial addition test ( only the first 50 trials ) ; processing speed using the wais - iii digit symbol and symbol search subtests , trail making test part a , and stroop color naming and word reading ; learning and memory using the hopkins verbal learning test - revised and the brief visuospatial memory test - revised ; verbal / language fluency using the controlled oral word association test , executive functioning using trail making test part b and stroop color - word interference test ; and motor speed using the grooved pegboard test .
raw scores were converted into demographically adjusted t scores , grouped by neurocognitive domain , and averaged to create domain t scores .
the vas contains 18 visual analogue lines with bipolar anchors with descriptors relating to energy and fatigue .
internal consistency of the vas in our sample was high ( cronbach  = 0.86 ) .
the total score from the vas - fatigue subscale was examined in the current study .
we used roieditor for preprocessing procedures according to a modified version of the active contour method and diffeomap .
white matter parcellation map was used to segment the brain into 130 regions based on anatomical labeling , which included both the gray and white matter . once these regions were extracted , the cortex and the surrounding white matter were segmented using an fa threshold of 0.25 .
we focused our analyses on regions of the corona radiata , insula , internal capsule , external capsule , striatum , fronto - occiptial fasciculus , cingulum , thalamus , hippocampus , and amygdala based on prior studies . for mrsi
, metabolites were quantitated in each voxel using the frequency - domain fitting routine called lc - model algorithm , which analyzes the in vivo brain spectrum as a linear combination of individual simulated metabolite spectra that constitute the basis set or prior knowledge .
we were able to quantify total naa_naag ( naa + naa glutamate ) , total choline ( gpc_pch ) , mi , and glutamate / glutamine ( glu_gln ) for regions of frontal white and gray matter , parietal white and gray matter , and basal ganglia ( see figure 2 ) .
the accuracy of the quantitation was characterized using cramer - rao lower bound ( crlb ) , and metabolite ratios with crlb < 30% were considered for further analysis .
no statistically significant differences were found for potential confounding variables such as age , years of education , ethnicity , and estimated premorbid functioning ( as measured by the wechsler test of adult reading ) ( all p values > 0.05 ) .
groups also did not differ in current drug use . between - groups analysis of variance ( anova )
was used to examine differences between hcv and control groups on our 31 outcome variables of interest : neuroimaging ( 23 variables ) , neurocognitive assessment ( 7 variables ) , and fatigue ( 1 variable ) .
outcome variables were all analyzed on a continuous scale and assumptions for performing anova and pearson correlations were checked prior to running analyses .
demographic and drug use characteristics of the sample we used the false discovery rate ( fdr ) to correct for multiple comparisons with our outcome measures .
fdr is sometimes preferred over family - wise error rate methods ( e.g. , bonferroni ) because it provides greater statistical power and reduces the chance of making type ii errors .
given our available sample size of 96 for the neurocognitive and fatigue data , adjusted  level of 0.01 ( for multiple comparisons ) , and power of 0.80 , we had enough power to detect medium effects ( f = 0.31 ) .
this is consistent with effect sizes reported in previous investigations that have examined neurocognitive differences between hcv+ patients and controls . for neuroimaging analyses , with a sample of 49
this is also consistent with the effect sizes reported by neuroimaging studies that have compared hcv+ patients to controls .
there were statistically significant group differences in fa in the striatum ( f1,48 = 8.49 , p < 0.005 ) , with the hcv group demonstrating higher fa levels than controls . while group differences in fa in the thalamus ( f1,48 = 4.6 , p = 0.04 ) and insula ( f1,48 = 3.86 , p = 0.05 ) emerged , these did not survive multiple comparisons correction . there were no statistically significant group differences in fa values among our other brain regions of interest ( see figure 1a and table e-2 ) .
proton magnetic resonance spectroscopy imaging ( mrsi ) regions of interest of frontal white , parietal white , and basal ganglia .
dti = diffusion tensor imaging ; fa = fractional anisotropy ; fdr = false discovery rate . among the hcv+ group ,
higher levels of fa in the striatum were correlated with poorer overall neuropsychological performance ( r29 = 0.45 , p = 0.01 ; r29 = 0.53 , p = 0.003 ) and the domain of language fluency ( r29 = 0.41 , p = 0.02 ) .
individuals with chronic hcv infection demonstrated greater md in the fronto - occiptal fasciculus ( f1,48 = 18.6 , p < 0.0001 ) and external capsule ( f1,48 = 10.8 , p = 0.002 ) than controls ( see figure 1b ) .
there was a statistical trend toward group md differences in the insula ( f1,48 = 3.86 , p = 0.05 ) ; however , this did not survive correction for multiple comparisons .
there were no statistically significant relationships between md in our regions of interest and neurocognitive performance .
hcv+ participants demonstrated lower levels of naa in bilateral parietal white matter ( f1,48 = 5.37 , p = 0.02 ) and elevations of mi in bilateral frontal white matter ( f1,48 = 9.48 , p = 0.004 ) compared to controls ( see figure 3 and table e-3 ) .
for the hcv+ group , greater levels of frontal white matter mi were significantly correlated with poorer cognitive performance in the domains of processing speed ( r29 = 0.43 , p = 0.02 ) and verbal / language fluency ( r29 = 0.41 , p = 0.007 ) .
higher naa in bilateral parietal white matter was significantly correlated with lower diffusivity in the fronto - occipital fasciculus ( r29 = 0.36 , p = 0.02 ) , whereas greater levels of frontal white matter mi were significantly correlated with higher diffusivity in the fronto - occipital fasciculus ( r29 = 0.47 , p = 0.01 ) .
glu_gln = glutamate + glutamine ; gpc_pch = gylcerophosphocholine + phosphocholine ( total choline ) ; mrsi = proton magnetic resonance spectroscopy imaging ; naa_naag = n - acetylaspertate + n - acetylaspartate glutamate .
hcv - infected individuals demonstrated poorer overall neurocognitive performance than controls ( f1,94 = 5.6 , p = 0.02 ) .
cognitive domains that contributed to global findings included reduced processing speed ( f1,94 = 3.91 , p = 0.04 ) and verbal fluency ( f1,94 = 8.52 , p = 0.005 ) ( see figure 4 ) .
neurocognitive performance differences between the hepatitis c group and controls . * = statistically significant at fdr - adjusted p value .
as expected , hcv+ participants reported greater fatigue ( f1,94 = 7.41 , p = 0.008 ) than controls . among the hcv+ group ,
fatigue was not correlated with any of the dti regions ( all p values > 0.05 ) .
higher fatigue scores correlated with higher levels of mi in frontal white matter ( r29 = 0.53 , p < 0.01 ) . among hcv+ patients , higher fatigue scores correlated with poorer overall neurocognitive performance ( r75 = 0.36 , p < 0.001 ) and domains of attention ( r75 = 0.26 , p = 0.01 ) , processing speed ( r75 = 0.31 , p = 0.002 ) , and executive functioning ( r75 = 0.40 , p < 0.001 ) .
there were statistically significant group differences in fa in the striatum ( f1,48 = 8.49 , p < 0.005 ) , with the hcv group demonstrating higher fa levels than controls . while group differences in fa in the thalamus ( f1,48 = 4.6 , p = 0.04 ) and insula ( f1,48 = 3.86 , p = 0.05 ) emerged , these did not survive multiple comparisons correction . there were no statistically significant group differences in fa values among our other brain regions of interest ( see figure 1a and table e-2 ) .
proton magnetic resonance spectroscopy imaging ( mrsi ) regions of interest of frontal white , parietal white , and basal ganglia .
dti = diffusion tensor imaging ; fa = fractional anisotropy ; fdr = false discovery rate . among the hcv+ group ,
higher levels of fa in the striatum were correlated with poorer overall neuropsychological performance ( r29 = 0.45 , p = 0.01 ; r29 = 0.53 , p = 0.003 ) and the domain of language fluency ( r29 = 0.41 , p = 0.02 ) .
individuals with chronic hcv infection demonstrated greater md in the fronto - occiptal fasciculus ( f1,48 = 18.6 , p < 0.0001 ) and external capsule ( f1,48 = 10.8 , p = 0.002 ) than controls ( see figure 1b ) .
there was a statistical trend toward group md differences in the insula ( f1,48 = 3.86 , p = 0.05 ) ; however , this did not survive correction for multiple comparisons .
there were no statistically significant relationships between md in our regions of interest and neurocognitive performance .
hcv+ participants demonstrated lower levels of naa in bilateral parietal white matter ( f1,48 = 5.37 , p = 0.02 ) and elevations of mi in bilateral frontal white matter ( f1,48 = 9.48 , p = 0.004 ) compared to controls ( see figure 3 and table e-3 ) .
for the hcv+ group , greater levels of frontal white matter mi were significantly correlated with poorer cognitive performance in the domains of processing speed ( r29 = 0.43 , p = 0.02 ) and verbal / language fluency ( r29 = 0.41 , p = 0.007 ) .
higher naa in bilateral parietal white matter was significantly correlated with lower diffusivity in the fronto - occipital fasciculus ( r29 = 0.36 , p = 0.02 ) , whereas greater levels of frontal white matter mi were significantly correlated with higher diffusivity in the fronto - occipital fasciculus ( r29 = 0.47 , p = 0.01 ) .
glu_gln = glutamate + glutamine ; gpc_pch = gylcerophosphocholine + phosphocholine ( total choline ) ; mrsi = proton magnetic resonance spectroscopy imaging ; naa_naag = n - acetylaspertate + n - acetylaspartate glutamate . * = statistically significant at fdr - adjusted p value .
hcv - infected individuals demonstrated poorer overall neurocognitive performance than controls ( f1,94 = 5.6 , p = 0.02 ) .
cognitive domains that contributed to global findings included reduced processing speed ( f1,94 = 3.91 , p = 0.04 ) and verbal fluency ( f1,94 = 8.52 , p = 0.005 ) ( see figure 4 ) .
neurocognitive performance differences between the hepatitis c group and controls . * = statistically significant at fdr - adjusted p value .
as expected , hcv+ participants reported greater fatigue ( f1,94 = 7.41 , p = 0.008 ) than controls . among the hcv+ group ,
fatigue was not correlated with any of the dti regions ( all p values > 0.05 ) .
higher fatigue scores correlated with higher levels of mi in frontal white matter ( r29 = 0.53 , p < 0.01 ) . among hcv+ patients , higher fatigue scores correlated with poorer overall neurocognitive performance ( r75 = 0.36 , p < 0.001 ) and domains of attention ( r75 = 0.26 , p = 0.01 ) , processing speed ( r75 = 0.31 , p = 0.002 ) , and executive functioning ( r75 = 0.40 , p < 0.001 ) . there was no statistically significant correlation between fatigue and motor functioning .
the current study examined the effects of chronic hcv infection on microstructural brain abnormalities , cerebral metabolites , fatigue , and neurocognitive performance .
major strengths of the current investigation include the use of dti and mrsi in combination with measures of neurocognitive functioning and fatigue , and the use of a control group for comparison . as hypothesized based on prior literature , we observed microstructural abnormalities in such areas as the striatum , external capsule , and fronto - occipital fasciculus , which is consistent with previous dti studies of hcv and findings among individuals with hiv infection .
we observed greater fa in gray matter regions of the striatum in hcv+ patients compared to healthy volunteers .
higher fa in the striatum has been found among patients with huntington disease and is thought to be due to degeneration of efferent pathways that increase the coherence of gray matter structures . in a study of patients with chronic subdural hematoma , increased fa was found in the striatum , which reduced following surgical intervention .
therefore , our findings are consistent with other investigations of neuropathology in regions that are affected in hcv
. increased diffusivity in the fronto - occipital tract and external capsule was also found in the hcv+ group compared to controls .
the fronto - occipital tract has been suggested as modulating frontal lobe  related inhibitory control and occipital lobe  related sensory inputs .
alterations of this tract may interfere with integrating sensory information and inhibiting control over impulses and emotion , which is problematic among drug abusers .
the external capsule contains a variety of different nerve bundles and pathways connecting the cerebral cortex to subcortical nuclei as well as connecting different parts of the cortex to each other .
therefore , disruption to fibers of the external capsule may result in dysfunction of frontal - subcortical circuitry .
hcv+ participants demonstrated lower levels of naa in bilateral parietal white matter and elevations of mi in bilateral frontal white matter compared to controls , which was associated with poorer performance in the cognitive domains of processing speed and verbal / language fluency .
specifically , higher naa in parietal white matter was significantly correlated with lower diffusivity in the fronto - occipital fasciculus , whereas greater frontal white matter mi was significantly correlated with higher diffusivity in the fronto - occipital fasciculus .
that stated , our mrsi results were generally consistent with previous mrsi studies of hcv+ cohorts , although we did not observe abnormal cerebral metabolite levels in basal ganglia as was expected .
however , in the current study we were careful to exclude participants with medical ( e.g. , cirrhosis ) and psychiatric conditions that potentially could have confounded interpretation of the neuroimaging findings . through this process
we may have excluded hcv+ individuals with more severe neurologic impairments and neuropathologic changes in subcortical structures that are detectable by h - mrs . although hcv+ patients demonstrated poorer global neurocognitive performance than controls , examination of performance data suggests normal range of performance ( i.e. , t > 40 ) . again , because of the use of stringent inclusion / exclusion criteria , this group may not be fully representative of the general hcv+ population . despite the potential recruitment of higher - functioning hcv+ individuals
, we still found the poorer performance in the cognitive domains of processing speed and verbal fluency ( relative to controls ) that has been reported across other studies , and this performance was independent of such factors as liver fibrosis and history of substance abuse .
participants also reported greater fatigue than controls , which was associated with abnormalities in frontal white matter , whereas poorer cognitive performance was associated with abnormalities in both frontal white matter and subcortical structures .
these results suggest that hcv - associated neurologic complications that are specific to changes in frontal - subcortical structures give rise to both reduced cognitive performance and fatigue .
the specific cognitive deficits observed in verbal / language fluency and information processing speed are all regulated by frontal - striatal structures . in our sample ,
verbal fluency demonstrated the greatest degree of performance difference between hcv+ and control groups and the strongest correlation with elevated levels of mi in frontal white matter .
first , while structural neuroimaging methods are helpful in identifying microstructural pathology that may not be detected on standard mri , they do not provide a clear understanding about the functions of these neural circuits .
hence , existing disruptions in a neural circuit may make a patient more vulnerable to developing symptoms such as fatigue .
second , although we attempted to control for a number of demographic variables between hcv patients and controls , we recognize that there are a myriad of psychosocial differences ( e.g. , stress , past drug use ) that may account for the reduced cognitive performance and structural brain differences that were observed in the current study .
for instance , we were unable to examine past drug abuse differences between our hcv+ and control groups because information on past drug abuse was not collected from the controls .
we recognize that in order to precisely rule out the effects of past drug abuse we would have needed to recruit a sample of past drug abusers who were hcv. however , considering that 61% of our hcv+ patients reported a lifetime history of cocaine or opiate use , we attempted to address this concern by examining the effects of past drug abuse within this subgroup .
while we did not find significant differences in our neuroimaging or neurocognitive data as a function of past drug abuse ( all p values > 0.10 ) , we can not rule out the residual confounding effects of distant substance use on neurologic function . despite these limitations ,
the current study represents a significant extension of the extant literature on hcv 's effects on neurologic and neurobehavioral functioning by demonstrating how abnormalities in frontal / parietal and subcortical structures have independent and overlapping relationships with cognitive performance and fatigue .
it has long been known that hcv is hepatotoxic ; increasingly there is reason to believe that it is neurotoxic as well . while the precise pathophysiologic mechanism remains unclear , findings from the current study as well as
while advances in the pharmacologic treatment of hcv hold incredible promise , there remain millions of hcv - infected adults in the united states and approximately 100 million worldwide .
thames was involved in the study design , was responsible for conducting statistical analyses of study hypotheses , assisted with processing of neuroimaging data , and drafted the initial version of the manuscript .
dr . castellon was involved in the conceptualization of the parent study ; provided scientific input on study design , data collection , and interpretation ; provided consultation for statistical analyses in the current study ; provided critical revisions to the write - up of study findings ; and gave final approval of the manuscript for submission .
dr . singer was involved in the conceptualization of the parent study design , provided scientific input on clinical and medical data collection and interpretation , provided critical revisions to the write - up of study findings , and gave final approval of the manuscript for submission .
nagarajan was involved in the study design of the mr spectroscopy data acquisition and mr spectroscopy postprocessing , provided critical revisions to the write - up of mr spectroscopy findings and interpretation , and gave final approval of the manuscript for submission .
sarma was involved in the study design of the diffusion tensor imaging data acquisition and processing , assisted with the analyses , provided critical revisions to the write - up of diffusion tensor imaging findings and interpretation , and gave final approval of the manuscript for submission .
smith was involved in the interpretation of clinical data , provided critical revisions to the manuscript draft , and gave final approval of the manuscript for submission .
thaler assisted with statistical analyses of the neuropsychological data , assisted with the write - up of results , provided critical revisions to manuscript drafts , and gave final approval of the manuscript for submission .
truong provided scientific input on medical data acquisition , provided critical revisions to the manuscript draft , and gave final approval of the manuscript for submission .
schonfeld was involved in data acquisition , formatted the tables and figures , assisted with the write - up of the methods for the manuscript , and gave final approval of the manuscript for submission .
thomas was responsible for the conceptualization and study design of the neuroimaging data , provided scientific input on sequence parameters and data analysis , provided critical revisions to manuscript drafts , and gave final approval of the manuscript for submission .
dr . hinkin is the pi of the parent project and was responsible for the conceptualization of the study design , provided scientific oversight for all study phases , provided critical portions of manuscript drafts , and gave final approval of the manuscript for submission .
funding support for the current study was provided through the nih ( ro1mh083553 , pi : c.h .
thames has received research support from the nimh and the american psychological association society for clinical neuropsychology .
e. singer is on the nih study section advisory board , is a reviewer for orau grants , and receives funding from nimh , minds , and nida .
 objective : this study examined neurologic abnormalities ( as measured by proton magnetic resonance spectroscopy imaging and diffusion tensor imaging ) , neurocognitive performance , and fatigue among a sample of adults with hepatitis c virus ( hcv ) . 
 we hypothesized that hcv+ individuals would demonstrate structural brain abnormalities and neurocognitive compromise consistent with frontostriatal dysfunction as well as increased fatigue compared to controls.method:participants were 76 individuals diagnosed with hcv and 20 controls who underwent a comprehensive neurocognitive evaluation and clinical assessments . 
 a subset of the hcv+ participants ( n = 29 ) and all controls underwent mri.results:individuals diagnosed with chronic hcv infection demonstrated greater fractional anisotropy in the striatum as well as greater mean diffusivity in the fronto - occiptal fasciculus and external capsule compared to hcv controls . 
 hcv+ participants also demonstrated lower levels of n - acetylaspartate in bilateral parietal white matter and elevations in myo - inosital ( mi ) in bilateral frontal white matter compared to hcv controls ( all p values < 0.05 ) . 
 hcv+ participants also demonstrated significantly poorer neuropsychological performance , particularly in processing speed and verbal fluency . 
 hcv+ patients reported higher levels of fatigue than controls , and fatigue was significantly correlated with diffusivity in the superior fronto - occipital fasciculus , elevations in mi in frontal white matter , and overall cognitive performance.conclusions:our results suggest that hcv - associated neurologic complications disrupt frontostriatal structures , which may result in increased fatigue and poorer cognitive performance , particularly in those cognitive domains regulated by frontostriatal regions . 
METHODS
Standard protocol approvals, registrations, and patient consents.
DTI and MRSI postprocessing.
Preliminary analyses.
RESULTS
DTI.
MRSI.
Neurocognition.
Fatigue.
DISCUSSION
Supplementary Material
AUTHOR CONTRIBUTIONS
STUDY FUNDING
DISCLOSURE