the study was conducted at the department of ophthalmology of a medical college in north - east india between september 2012 and june 2014 .
approval from the institute 's ethical committee was obtained , and the study was carried out in accordance with the ethical standards of the 1964 declaration of helsinki .
the inclusion criteria were as follows : ( 1 ) patients with unilateral csc , either acute or chronic ; and ( 2 ) patients who were willing to provide written informed consent .
exclusion criteria were as follows : ( 1 ) patients with both acute and chronic csc together in one or both eyes ; ( 2 ) patients who had a history of using corticosteroids , steroid hormones in any form - systemic steroids ( like oral / intravenous / intramuscular ) , topical eye drops , skin creams , intranasal / inhalational sprays , etc .
, in the past one month ; ( 3 ) any recent surgery or trauma ; ( 4 ) alcohol abuse or dependence ; ( 5 ) major depression ; and ( 6 ) other major eye or systemic diseases .
the refractive status was determined with both automated and retinoscopic methods , using appropriate cycloplegia when needed .
detailed slit - lamp biomicroscopy and indirect ophthalmoscopy were conducted after the pupils had been dilated .
fundus fluorescein angiography ( ffa ) was carried out using a fundus camera visucam lite ( carl zeiss meditec ag , jena , germany ) and optical coherence tomography ( oct ) with spectral domain oct ( model 500 ; cirrus hd - oct , carl zeiss meditec , dublin , ca , usa ) .
the cases were classified as acute or chronic depending on the clinical , ffa and oct findings .
an acute case of csc was defined as the first episode of csc that had symptom duration of less than three months with a well - circumscribed round or oval area of serous elevation of the neurosensory retina at the macular region .
these acute cases also showed dye leakage either as a single leak or multiple leaks on ffa , with the leakage pattern being either the inkblot type or the smokestack type , as well as the presence of serous neurosensory retinal detachments either with or without rpe detachments on oct .
chronic csc was defined as csc that had persisted for more than three months and showed clinical elevation of the neurosensory retina with fine rpe changes , like mottling and atrophy with pigmentation , revealing window defects with small leaks and mottled atrophic changes of rpe of a non - specific pattern on ffa and the presence of serous neurosensory retinal detachments with or without rpe detachments on oct .
estimations of the 8:00 to 9:00 a.m. serum cortisol and testosterone levels were carried out in all the patients using a chemiluminescent immunoassay method ( beckman coulter , fullerton , ca , usa ; supplied by beckman coulter india pvt .
ltd . , mumbai , india ) ; 5 ml blood were collected in a plain vial and allowed to clot for 15 minutes at room temperature .
the serum was separated by centrifuging the sample at the rate of 2,500 rpm for ten minutes .
the serum was used for the analysis of cortisol and total testosterone levels in an automated access 2 immunoassay system ( beckman coulter ) as per the manufacturer 's protocol .
proper standardization and internal quality control were ascertained as per these guidelines before the assays were conducted .
the normal reference levels of serum cortisol and testosterone at our laboratory were determined as 6.70 to 22.60 g / dl and 1.75 to 7.81 ng / dl , respectively .
an independent sample t - test was employed to identify any difference between the two groups .
a partial correlation test was used to determine the difference in the levels of serum cortisol and testosterone by controlling for the effect of age and gender . a 5% level of significance
an acute case of csc was defined as the first episode of csc that had symptom duration of less than three months with a well - circumscribed round or oval area of serous elevation of the neurosensory retina at the macular region .
these acute cases also showed dye leakage either as a single leak or multiple leaks on ffa , with the leakage pattern being either the inkblot type or the smokestack type , as well as the presence of serous neurosensory retinal detachments either with or without rpe detachments on oct .
chronic csc was defined as csc that had persisted for more than three months and showed clinical elevation of the neurosensory retina with fine rpe changes , like mottling and atrophy with pigmentation , revealing window defects with small leaks and mottled atrophic changes of rpe of a non - specific pattern on ffa and the presence of serous neurosensory retinal detachments with or without rpe detachments on oct .
estimations of the 8:00 to 9:00 a.m. serum cortisol and testosterone levels were carried out in all the patients using a chemiluminescent immunoassay method ( beckman coulter , fullerton , ca , usa ; supplied by beckman coulter india pvt . ltd . , mumbai , india ) ; 5 ml blood were collected in a plain vial and allowed to clot for 15 minutes at room temperature .
the serum was separated by centrifuging the sample at the rate of 2,500 rpm for ten minutes .
the serum was used for the analysis of cortisol and total testosterone levels in an automated access 2 immunoassay system ( beckman coulter ) as per the manufacturer 's protocol .
proper standardization and internal quality control were ascertained as per these guidelines before the assays were conducted .
the normal reference levels of serum cortisol and testosterone at our laboratory were determined as 6.70 to 22.60 g / dl and 1.75 to 7.81 ng / dl , respectively .
an independent sample t - test was employed to identify any difference between the two groups .
a partial correlation test was used to determine the difference in the levels of serum cortisol and testosterone by controlling for the effect of age and gender . a 5% level of significance
a summary of the characteristics of the patients along with their serum cortisol and testosterone levels is given in table 1 .
the mean age was 42.43  6.37 years ( range , 32 to 56 years ) .
the mean 8:00 to 9:00 a.m. serum cortisol level was 12.61  4.74 g / dl ( range , 6.58 to 27.42 g / dl ) .
the mean serum testosterone level was 5.88  1.57 ng / dl ( range , 2.81 to 9.94 ng / dl ) .
the mean visual acuity was 20 / 65.07  40.56 ( range , 20 / 25 to 20 / 200 ) .
there was no statistically significant difference in the mean levels of serum cortisol and testosterone between the acute and chronic cases ( p > 0.05 ) , but there was a statistically significant difference in the mean presenting visual acuity in the two groups ( p < 0.05 ) .
no statistically significant difference in the levels of these hormones could be found by controlling for the effect of age ( p = 0.937 for serum cortisol and p = 0.545 for testosterone ) and gender ( p = 0.963 for serum cortisol and p = 0.491 for testosterone ) using a partial correlation test .
clinically , acute cases revealed a well - circumscribed round or oval area of serous elevation of neurosensory retina at the macular region .
chronic cases showed a mild elevation of the neurosensory retina with fine rpe changes , like mottling and atrophy with pigmentation .
ffa revealed leakage of dye either as a single leak or multiple leaks in acute csc .
chronic cases showed window defects with small leaks and mottled atrophic changes of the rpe with a nonspecific pattern ( fig .
csc is an idiopathic disease characterized by serous detachment of the neurosensory retina in the macular region secondary to a focal retinal pigment epithelial defect .
risk factors are type a personality , corticosteroid use in various forms , age , male gender , increased endogenous cortisol production , systemic lupus erythematosus , pregnancy , antibiotic use , alcohol use , allergic respiratory disease , untreated hypertension , psychopharmacologic medication , organ transplantation , hemodialisis , and cortisol - producing tumors .
it is usually self - limited with spontaneous resolution at four to six months , but a few cases may progress to chronic disease with prolonged serous macular detachment .
csc is said to predominantly affect young and middle - aged males between 20 and 50 years , and its incidence declines with advancing age . however , there are reports of csc occurring in patients 60 years or older . in our study , the age of patients ranged from 32 to 56 years ( mean , 42.43  6.37 years ) .
the treatment of csc is not established uniformly . in a retrospective follow - up study , gilbert et al
even centuries after von graefe described recurrent serous detachment of the macula as " recurrent central retinitis " in 1866 , the etiology of this condition continues to elude clinicians despite active research .
determined the endogenous cortisol levels in urine and plasma samples in 30 patients with acute csc and compared them with 30 age and sex matched controls .
they found that the mean plasma and urine cortisol levels were significantly higher in patients with acute csc compared to age - matched controls .
similarly , kapetanios et al .   evaluated the levels of endogenous cortisol in 16 patients with acute csc and compared them with the age and sex matched control group .
however , chalisgaonkar et al .   did not find a precise correlation of the serum cortisol with csc .
similarly , tufan et al .   could not identify any correlation of the serum cortisol with chronic csc . from these studies
, it can be inferred that there is an inconsistent relationship between the levels of serum cortisol and csc . in our study , 30 patients with either acute or chronic csc were evaluated . there were 17 cases with acute csc ( 56.7% ) and 13 cases with chronic csc ( 43.3% ) .
all except case no . 6 ( 96.67% ) had serum cortisol levels within the normal range .
.   identified corticosteroid use as a risk factor associated with csc that was distinct from psychopharmacologic medication use and hypertension in their retrospective study of 230 consecutive patients with csc .
similarly , in an observational case series of 24 patients , haimovici et al .   reported that the serum testosterone levels were within the normal range in 23 of their 24 patients .
were the first to estimate the serum testosterone levels in patients suffering from csc and compare them with age and sex matched controls .
the mean serum levels of testosterone were observed to be within the normal range in both csc patients and controls .
however , they found 32% of csc patients and 18% of controls to have lower than normal levels of testosterone . in their study , tufan et al .   reported serum testosterone levels within the normal range in patients with chronic csc
they concluded that the association between these hormones and chronic csc might be weak . in our study , all cases ( 100% ) had serum testosterone levels in the normal range .
we carried out the present study because no study thus far has revealed a difference in the levels of serum cortisol and testosterone in acute and chronic csc .
if a difference is found , it might shed some further light on the etiopathogenesis of csc and explain why some patients with csc have recurrences whereas others do not .
however , we did not find any statistically significant difference in the levels of serum cortisol and testosterone in acute and chronic csc , even after controlling for the effects of age and gender . in conclusion
, it may be inferred from our study that there is unlikely to be any statistically significant difference in the mean levels of serum cortisol and testosterone between the acute and chronic cases of csc , but there may be a statistically significant difference in the mean presenting visual acuity in the two groups .
 purposeto compare the levels of serum cortisol and testosterone in acute and chronic central serous chorio - retinopathy ( csc).methodsserum cortisol and testosterone levels in 30 patients with either acute or chronic csc were evaluated using chemiluminescent immunoassay.resultsthe mean age was 42.43  6.37 years ( range , 32 to 56 years ) . 
 the mean 8:00 to 9.00 a.m. serum cortisol level was 12.61  4.74 g / dl ( range , 6.58 to 27.42 g / dl ) . 
 the mean serum testosterone level was 5.88  1.57 ng / dl ( range , 2.81 to 9.94 ng / dl ) . 
 the mean visual acuity was 20 / 65.07  40.56 ( range , 20 / 25 to 20 / 200 ) . 
 there was no statistically significant difference in the mean levels of serum cortisol and testosterone between the acute and chronic cases ( p > 0.05 ) , but there was a statistically significant difference in the mean presenting visual acuity in the two groups ( p < 0.05).conclusionsall except one patient in the acute group had normal levels of serum cortisol . 
 testosterone levels were within the normal range in both the acute and chronic cases of csc . 
 there is unlikely to be any statistically significant difference in the mean levels of serum cortisol and testosterone between the acute and chronic cases , but there may be a statistically significant difference in the mean presenting visual acuity in these groups . 
Materials and Methods
Definitions for acute and chronic central serous chorio-retinopathy in the study
Statistical analysis
Results
Discussion