the developing vertebrate heart tube contains an outer layer of myocardium and an inner layer of endocardium separated by an extracellular matrix ( ecm ) referred to as the cardiac jelly . during heart valve formation
, a subset of endocardial cells in the atrioventricular ( av ) canal ( avc ) region is specified to delaminate , differentiate , and migrate into the cardiac jelly in a process called endocardial - mesenchymal transition ( emt ) ( armstrong and bischoff , 2004 ; combs and yutzey , 2009 ) .
for av valve formation , locally swollen cardiac jelly together with mesenchymal cells derived from avc endocardial cells form the endocardial cushion ; however the extensive remodeling process that occurs subsequently is not clearly understood ( armstrong and bischoff , 2004 ; combs and yutzey , 2009 ) .
signal molecules produced from both the myocardium and endocardium in the avc region are necessary for proper endocardial cushion formation and emt of endocardial cells ( krug et al . , 1985 ) . in the initial step of endocardial cushion formation ,
a bone morphogenetic protein ( bmp ) signal from avc myocardium inhibits the expression of chamber - specific genes in the avc region , while the endocardial cushion is enlarged by increased deposition of ecm or cardiac jelly between the myocardium and endocardium layers in the avc . upon receiving a myocardially derived bmp signal ,
a subset of avc endocardium cells undergo the emt process by producing multiple signals such as transforming growth factor  , notch , and wnt/-catenin , and then migrate into the cardiac jelly where they transition into mesenchymal cells that form valve precursors during later development ( armstrong and bischoff , 2004 ; combs and yutzey , 2009 ; dlot , 2003 ; nakajima et al . , 2000 ) .
therefore , deficiencies in bmp or notch signal - related genes inhibit or disrupt normal endocardial cushion formation and/or failure of avc endocardium emt in the mouse , chick , and zebrafish ( jiao et al . , 2003 ; ma et al . , 2005 ;
in addition , fgfs , vegfs , nfat , and hdac are also prerequisites for normal heart valve development ( combs and yutzey , 2009 ; kim et al . , 2012b ) .
glycosaminoglycan hyaluronan exists as a hydrated gel that expands the extracellular space , regulates ligand availability , and interacts with numerous ecm components including versican , a major constituent of cardiac jelly ( armstrong and bischoff , 2004 ) .
disruption of hyaluronan synthase 2 ( has2 ) or versican in mice and zebrafish is reported to abrogate avc endocardial cushion formation and emt - associated mesenchymal cell migration , suggesting that an appropriate ecm environment is important for both emt of avc endocardium development and formation of the endocardial cushion ( camenisch et al . , 2000 ;
although there is some debate on whether emt is a prerequisite for the migration of avc endocardial cells into the cardiac jelly during endocardial cushion formation in zebrafish , the mature av valve of the 2-chambered zebrafish adult heart and 4-chambered mammalian valves are structurally similar .
in addition , cellular and molecular signaling events occurring during heart valve development are largely conserved between zebrafish and mammals ( beis et al .
cysteine - rich protein 2 ( crip2 ) is a crip - type subgroup of the lim domain protein family and has two conserved cysteine - rich motifs that contain cysteine and histidine residues with zinc - binding properties ( karim et al . , 1996 ; tsui et al .
mouse crip2 is reported to be highly expressed in the heart , brain , testis , kidney , and lung , and is involved in the dynamics of the cortical actin cytoskeleton by binding ptp - bl , a protein tyrosine phosphatase ( van ham et al . , 2003 ) . in zebrafish , crip2 is reported to be a target of wnt3a signaling and regulates smooth muscle cell differentiation ( kihara et al . , 2011 ) and the development of cardiac neural crest cells during early embryogenesis ( sun et al . , 2008 ) .
however , the direct role of crip2 in the av valve formation has not yet been elucidated .
zebrafish is a useful animal model for studying development of the heart and other circulation systems , including blood and lymphatic vessels ( kim and kim , 2014 , kim et al .
, 2012a ; 2013a ; 2013b ; ) because the embryos are transparent and a multitude of transgenic lines that express fluorescent proteins in tissue- and cell - specific manners are readily available . in zebra - fish ,
the heart can be observed under a dissecting microscope soon after formation of the primitive heart tube .
the heart starts beating at 24 h post - fertilization ( hpf ) , heart looping begins at 36 hpf , functional valves are formed by 48 hpf , and heart valve development is complete by approximately 55 hpf ( stainier et al . , 2002 ) .
in this study , we found that crip2 expression in the avc endocardial cells , but not in the avc myocardial cells , in late heart development is critical for heart valve development in zebrafish embryos .
furthermore , crip2-deficient embryos showed markedly increased expression of the has2 and versican a genes , which are responsible for the synthesis of versican a and hyaluronan .
we provide data that crip2 expressed in the avc endothelial cells plays an important role in cardiac valve formation by suppressing genes involved in the synthesis of versican a and hyaluronan , which are essential ecm components in the cardiac jelly and endocardial cushion .
zebrafish ( danio rerio ) were raised and maintained at 28.5c , and embryos were staged as described in a previous study ( kimmel et al . , 1995 ) .
embryos were obtained from spontaneous spawnings and the embryos were manually dechorionated at appropriate stages by using watchmaker s forceps and then fixed using 4% paraformaldehyde in phosphate - buffered saline .
tg ( cmlc2:egfp ) ( huang et al . , 2003 ) and tg ( flk1:egfp ) ( jin et al . , 2005 ) embryos were used for phenotypic analyses and immunostaining .
antisense digoxigenin - labeled rna probes for crip2 , vmhc , cmlc2 , cdh5 , bmp4 , tbx2b , notch1b , versican a , and has2 were generated using in vitro transcription with t3 , t7 , or sp6 rna polymerase , according to the manufacturer s instructions ( roche , germany ) .
whole - mount in situ hybridization was performed at the desired embryonic stages , as described by jowett and lettice ( 1994 ) . for fluorescent immunostaining
after in situ hybridization , transgenic embryos were immunostained by anti - gfp antibody ( santa cruz biotechnology , inc .
processed embryos were photographed using a microscope ( axioskop , zeiss , germany ) outfitted with a nikon coolpix 4500 digital camera system and a laser - scanning confocal microscope ( lsm 5 pascal ) .
crip2 antisense morpholino oligonucleotide ( crip2 mo ) was designed and ontained from gene tools llc .
( usa ) to block the splicing of crip2 exon 2 and intron 2 and had the following sequence : 5-catgccgaggtgagtaaaacagaga-3. to generate crip2 sense mrna , the full open reading frame of crip2 ( nm_213497 ) was cloned into the pcs2 + expression vector .
the construct was linearized using noti digestion and capped rna was synthesized using mg ( 5 ) ppp ( 5 ) g ( roche ) and sp6 rna polymerase ( roche ) .
the rna was diluted to 100 ng/l and phenol red was added to a final concentration of 0.2% immediately before injection .
the synthesized crip2 mo and sense mrna were injected into one- or two - cell stage embryos by using a gas driven microinjector ( world precision instruments , usa ) .
zebrafish ( danio rerio ) were raised and maintained at 28.5c , and embryos were staged as described in a previous study ( kimmel et al . , 1995 ) .
embryos were obtained from spontaneous spawnings and the embryos were manually dechorionated at appropriate stages by using watchmaker s forceps and then fixed using 4% paraformaldehyde in phosphate - buffered saline .
tg ( cmlc2:egfp ) ( huang et al . , 2003 ) and tg ( flk1:egfp ) ( jin et al . , 2005 ) embryos were used for phenotypic analyses and immunostaining .
antisense digoxigenin - labeled rna probes for crip2 , vmhc , cmlc2 , cdh5 , bmp4 , tbx2b , notch1b , versican a , and has2 were generated using in vitro transcription with t3 , t7 , or sp6 rna polymerase , according to the manufacturer s instructions ( roche , germany ) .
whole - mount in situ hybridization was performed at the desired embryonic stages , as described by jowett and lettice ( 1994 ) . for fluorescent immunostaining
after in situ hybridization , transgenic embryos were immunostained by anti - gfp antibody ( santa cruz biotechnology , inc .
processed embryos were photographed using a microscope ( axioskop , zeiss , germany ) outfitted with a nikon coolpix 4500 digital camera system and a laser - scanning confocal microscope ( lsm 5 pascal ) .
crip2 antisense morpholino oligonucleotide ( crip2 mo ) was designed and ontained from gene tools llc .
( usa ) to block the splicing of crip2 exon 2 and intron 2 and had the following sequence : 5-catgccgaggtgagtaaaacagaga-3. to generate crip2 sense mrna , the full open reading frame of crip2 ( nm_213497 ) was cloned into the pcs2 + expression vector .
the construct was linearized using noti digestion and capped rna was synthesized using mg ( 5 ) ppp ( 5 ) g ( roche ) and sp6 rna polymerase ( roche ) .
the rna was diluted to 100 ng/l and phenol red was added to a final concentration of 0.2% immediately before injection . the synthesized crip2 mo and sense mrna were injected into one- or two - cell stage embryos by using a gas driven microinjector ( world precision instruments , usa ) .
in an in situ hybridization - based screen for candidate genes that regulate heart valve development , we found that crip2 expression was restricted to the avc region in 48-hpf zebrafish embryos ( fig . 1 and data not shown ) .
detailed analysis of the gene expression profile showed that crip2 mrna was detected in zebrafish embryos at the one - cell stage , indicating that crip2 was maternally expressed ( fig .
at the shield stage , crip2 was ubiquitously expressed in the entire embryonic cell ( fig .
1b ) , but its expression became restricted to the mesodermal region along the anterior - posterior axis at the 10 somite - stage ( ss ) ( fig .
crip2 expression became further restricted to the head and trunk mesoderm , including the somites , lateral plate mesoderm , and notochord posterior edge ( fig .
transverse sections of the posterior trunk region further confirmed its expression in the somitic muscle ( fig .
1h ) , and new expression appeared in the pharyngeal arch blood vessels ( fig .
these gene expression profiling data suggest a function of zygotic crip2 in mesoderm - derived tissue formation during early embryogenesis . during heart development , crip2 expression was observed in the linear heart tube in 24 hpf embryos ( fig .
1k ) and its strong expression persisted in the ventricle and av canal regions at 36 hpf , but was barely detected in the atrium ( fig .
crip2 expression was decreased in the ventricle and remained only at the avc and outflow tract ( figs .
when heart valve development was completed at 55 hpf , crip2 expression at the avc and outflow tract had started to decrease ( fig .
1o ) . in tg(flk1:egfp ) transgenic zebrafish embryos , green fluorescent protein ( gfp ) is expressed in all endothelial cells ( jin et al . , 2005 ) .
to verify which cells express crip2 , 48 hpf tg ( flk1:egfp ) embryos were immunostained with gfp antibodies and then whole - mount in situ hybridization was performed using a fluorescent crip2 riboprobe .
endocardial cells in the ventricle , atrium , and avc region were visualized using the gfp antibody ( fig .
1p ) , and expression of crip2 was detected in cells at the avc and outflow tract ( fig .
1p and 1q , we confirmed that crip2 is expressed specifically in the endocardium of the avc and outflow tract and not in the heart chamber myocardium or endocardium during the late stage of heart development ( figs .
to evaluate the contribution of crip2 to late heart development , we designed a crip2 splice morpholino ( crip2 mo ) ( fig .
the crip2 mo effectively blocked splicing between exon 2 and intron 2 and resulted in a decrease of crip2 mrna transcript ( fig .
2b , * * ) and an increase in the aberrant transcript containing intron 2 ( fig . 2b , * ) in 24 hpf embryos .
similarly , the abundance of the crip2 mrna transcripts was clearly reduced by the crip2 mo ( figs .
2d and 2d ) in 24 hpf embryos when compared to non - injection control embryos ( nics ; figs .
2c and 2c ) examined using whole - mount in situ hybridization . to analyze the effect of crip2 knockdown on heart tube formation , we used tg ( cmlc2:egfp ) transgenic embryos that express gfp in myocardial cells in both ventricular and atrial portions of the heart tube ( huang et al . , 2003 )
. depending on the amount of crip2 mo injected into one - cell stage embryos , three variable phenotypes were induced in 48 hpf embryos : normal ( fig .
2g ) and typical ring structures in the avc region ( arrow ) were observed ( fig .
2 g ) . however , typical ring structures were not formed in the type 1 and type 2 embryos because heart tubes failed to undergo looping ( figs . 2 h , 2h , 2j , and 2j ) . because type 2 morphant embryos ( fig .
2i ) showed more severe developmental defects than type 1 morphants , type 1 embryos were selected for further analysis of crip2 function in heart development .
the ratios of the dose - dependent phenotypes were calculated and these data are summarized in fig .
2k . collectively , our data demonstrating that morphological heart valve defects occur in crip2 morphant embryos and that crip2 expression becomes spatiotemporally restricted to the endocardium of the avc and outflow tract strongly suggest an important role for crip2 in heart valve development . to further assess the function of crip2 in late heart development , including heart valve formation
cardiac myosin light chain 2 ( cmlc2 ) is expressed in myocardia throughout both ventricular and atrial portions of the heart tube , whereas ventricular myosin heavy chain ( vmhc ) is restricted to the myocardia in the ventricular portion ( yelon et al . , 1999 ) .
ve - cadherin ( cdh5 ) , on the other hand , is specifically expressed in the endocardium during heart development ( larson et al . , 2004 ) . in zebrafish embryos ,
the total abundance of cdh5 mrna was also not changed by crip2 mo ( figs . 3e and 3f ) , suggesting that crip2 is not involved in the development of myocardium and endocardium comprising the outer layers of heart chambers .
next , we examined the expression of genes required for the development of the heart valve .
bmp4 is known to be highly expressed specifically in the avc myocardium in zebrafish embryos ( larson et al . , 2004 ) .
in the crip2 morphant embryos , bmp4 was expressed in the avc region ( fig .
3h , arrows ) , in a manner similar to that of the control embryos ( fig .
3 g ) . in agreement with this , expression of tbx2b , a marker of the avc myocardium ( walsh and stainier , 2001 ) , was also not altered ( figs . 3i and 3j , arrows ) . in mice
, notch promotes emt during heart valve development and inhibition of notch activity prevents valve formation in mice and zebrafish ( timmerman et al . , 2004 ) .
notch1b , a mouse notch1 ortholog , was expressed in the avc endocardium of 48 hpf zebrafish embryos ( hurlstone et al .
, 2003 ) , thus we tested the expression of notch1b in crip2 morphant embryos .
we found that the expression level of notch1b detected in the avc region of morphant embryos was comparable to that of control embryos at 48 hpf ( figs . 3k and 3l ) .
this observation indicated that crip2 is not involved in regulating the emt process in the avc endocardium .
an appropriate ecm environment in the endocardial cushion is reported to be required for normal heart valve formation ( camenisch et al .
in zebrafish , it is well established that versican a and has2 synthesize versican a and hyaluronan , respectively , and that these genes have restricted expression in the avc region in 48 hpf embryos ( hurlstone et al . , 2003 ) .
to further investigate whether crip2 regulates synthesis of ecm components , we analyzed the expression of versican a and has2 in crip2 morphant embryos .
the expression of versican a in the avc region was significantly increased in the morphant embryos ( fig .
4a ) . similar to versican a , the expression of has2 in the avc region was markedly upregulated by the knockdown of crip2 ( figs . 4c and 4d ) .
these results imply that crip2 functions as a negative regulator of ecm - related gene expression during av valve development .
we also tested whether overexpression of crip2 could down - regulate versican a and has2 expression .
in wild - type embryos , overexpression of crip2 did not decrease the expression of versican a and has 2 , although severe developmental defects , including curled body and cardiac edema , were observed ( supplementary fig .
these results suggested that the expression level of crip2 in wild - type embryos is sufficient to suppress the expression of versican a and has 2 ; thus , further suppression of these ecm genes could not be achieved by the overexpression of crip2 .
alternatively , crip2 might require a cofactor to suppress versican a and has 2 expression .
therefore , overexpression of crip2 alone can not further suppress the expression of versican a and has 2 .
adenomatous polyposis coli ( apc ) , a well - known tumor suppressor protein , negatively regulates wnt/-catenin signaling ( hurlstone et al . , 2003 ) .
moreover , heart valve defects in apc null mice are reported to be caused by increased versican a and has2 expression in the avc region , indicating that versican a and has2 are transcriptional targets of the wnt/-catenin pathway ( hurlstone et al .
according to our crip2 knockdown data , expression of versican a and has2 at the avc was markedly upregulated , suggesting an antagonistic role of crip2 towards wnt/-catenin signaling that activates versican a and has2 expression .
our results , together with that of previous reports , indicate that either deficiency or over - production of versican and has2 causes heart valve defects ( hurlstone et al . , 2003 ; schroeder et al . ,
, we report a novel function of crip2 in suppressing the expression of versican a and has2 in the avc region , which is essential for normal heart valve development . through its spatiotemporal regulation of gene expression ,
crip2 likely contributes to the formation of an appropriate ecm environment in the endocardial cushion region during multiple stages of heart valve development .
 the initial step of atrioventricular ( av ) valve development involves the deposition of extracellular matrix ( ecm ) components of the endocardial cushion and the endocardialmesenchymal transition . 
 while the appropriately regulated expression of the major ecm components , versican and hyaluronan , that form the endocardial cushion is important for heart valve development , the underlying mechanism that regulates ecm gene expression remains unclear . 
 we found that zebrafish crip2 expression is restricted to a subset of cells in the av canal ( avc ) endocardium at 55 hours post - fertilization ( hpf ) . 
 knockdown of crip2 induced a heart - looping defect in zebrafish embryos , although the development of cardiac chambers appeared to be normal . in the avc of crip2-deficient embryos , the expression of both versican a and hyaluronan synthase 2 ( has2 ) was highly upregulated , but the expression of bone morphogenetic protein 4 ( bmp4 ) and t - box 2b ( tbx2b ) in the myocardium and of notch1b in the endocardium in the avc did not change . taken together , 
 these results indicate that crip2 plays an important role in av valve development by downregulating the expression of ecm components in the endocardial cushion . 
INTRODUCTION
MATERIALS AND METHODS
Maintenance of zebrafish and collection of embryos
Whole-mount 
Antisense morpholino oligonucleotide (MO) and sense mRNA injections
RESULTS AND DISCUSSION
Supplementary