this study was conducted during 14 months ( october 2014 to november 2015 ) on patients with ag ( n=39 ) and gc ( n=40 ) at two medical oncology and gastroenterology facilities in the northwestern region of algeria .
infection with h. pylori was confirmed by histopathology of biopsies sampled during diagnostic endoscopy ( at least four biopsies of the antrum and body gastric mucosa should be available for each case ) and anti - hp igg serum antibody tests .
the corresponding tissue sections were stained for slide reading in the anatomopathology department of the university hospital of sidi - bel - abbes , algeria .
the existence of h. pylori dna in gastric biopsies and buccal swabs were analyzed by pcr amplification of the urec gene using primers as described by lage et al .
( 16 ) ( forward : 5-aagcttttaggggtgttaggggttt-3 and reverse : 5-aagcttactttctaacactaacgc-3 ) at a hybridization temperature of 55c .
we employed a dna volume of 25 l according to pcr criteria , as described below .
the amplified fragments were visualized by using the gelred nucleic acid gel stain , 10,000 x ( biotium , hayward , ca , usa ) , and 2.5% agarose gel .
information about personal data , sociodemographic characteristics , educational level , blood type , rhesus factor , eating habits , smoking , and alcohol consumption were evaluated using a predetermined questionnaire .
furthermore , all participants gave their consent after the study protocol had been explained to them .
387 ( article 25 ) dated 31 july 2006 about ethical trials in algeria , we obtained the required access authorizations to the concerned health facilities in order to accomplish our study protocol .
furthermore , we obtained informed written consent from all participants and their treating physicians after the study protocol had been explained to them . il-1-31 and il-1-511 polymorphisms are single nucleotide changes in the promoter region , while the il-1rn polymorphism is a repetition of 86 bp in the second intron .
the dna was extracted from 200 l whole blood samples , 20 mg of fresh gastric biopsy , or buccal swab in h. pylori transport medium using an extraction kit ( magazorbe genomic dna , promega , fitchburg , wisconsin , usa ) stored at 20c .
genotyping of the two polymorphisms ( il-1-31 and il-1-511 ) was performed using the restriction fragment length polymorphism method ( pcr - rflp ) ( table 1 ) ( 6 , 10 ) .
briefly , 2550 ng of dna was used in a volume of 25 l with 150 mm dntp , 6.25 pmol of each primer , 1 u of dna polymerase gotaq g2 hot start polymerase ( promega , fitchburg , wisconsin , usa ) , 5% dmso , 4 mm mgcl2 , and 5 l of 5x pcr buffer gotaq .
the amplified / digested fragments were visualized with the gelred nucleic acid gel stain , 10,000 x ( biotium , hayward , ca , usa ) in 2.5% agarose gel . primers and conditions for analysis of the il-1 and il-1rn polymorphisms
tm : melting temperature ; il-1-31 : cluster of gene encoding interleukin-1 in the promoter region of tata box at the 31 position ; il-1-511 : cluster of gene encoding interleukin-1 in the promoter region of tata box at the 511 position ; il-1rn : gene encoding the anti - inflammatory antagonist ( interleukin-1ra ) .
variable numbers of tandem repeats ( vntr ) analysis in the second intron of the il-1rn gene was carried out by pcr .
the different alleles were identified by the size of the pcr fragments and encoded in a conventional manner ( 6 , 13 ) as follows : allele 1 ( four repetitions ) , allele 2 ( two repetitions ) , allele 3 ( five repetitions ) , allele 4 ( three repetitions ) , and allele 5 ( six repetitions ) . according to previous studies ( 6 , 13 ) , as described in table 1 , the il-1rn alleles were dichotomized into two categories : long genotype ( l:>three repetitions ; alleles 1 , 3 , 4 , 5 ) and short genotype ( 2 : two repetitions ; allele 2 ) .
data were processed and analyzed using spss 22.0 ( statistical package for the social sciences , ibm corporation ; chicago , il , august 2013 ) .
chi - square test was used to evaluate differences in the distribution of polymorphisms between different groups .
all allelic distributions were examined for the corresponding differences of their hardy - weinberg equilibrium .
the relative risk was evaluated by multivariate logistic regression based on categorical reference predictor models , adjusting for sex and age with a confidence interval ( ci ) of 95% and statistical significance set at p<0.05 .
allelic combinations of models of the three variants closely related polymorphisms of il-1 and il-1rn were analyzed .
the study of the haplotypes and linkage disequilibrium between loci ( estimated by r 
  and d ) was carried out via the web interface in the linkdos ( www.genepop.curtin.edu.au/linkc.html ) .
this study was conducted during 14 months ( october 2014 to november 2015 ) on patients with ag ( n=39 ) and gc ( n=40 ) at two medical oncology and gastroenterology facilities in the northwestern region of algeria .
infection with h. pylori was confirmed by histopathology of biopsies sampled during diagnostic endoscopy ( at least four biopsies of the antrum and body gastric mucosa should be available for each case ) and anti - hp igg serum antibody tests .
the corresponding tissue sections were stained for slide reading in the anatomopathology department of the university hospital of sidi - bel - abbes , algeria .
the existence of h. pylori dna in gastric biopsies and buccal swabs were analyzed by pcr amplification of the urec gene using primers as described by lage et al .
( 16 ) ( forward : 5-aagcttttaggggtgttaggggttt-3 and reverse : 5-aagcttactttctaacactaacgc-3 ) at a hybridization temperature of 55c .
we employed a dna volume of 25 l according to pcr criteria , as described below .
the amplified fragments were visualized by using the gelred nucleic acid gel stain , 10,000 x ( biotium , hayward , ca , usa ) , and 2.5% agarose gel .
information about personal data , sociodemographic characteristics , educational level , blood type , rhesus factor , eating habits , smoking , and alcohol consumption were evaluated using a predetermined questionnaire .
furthermore , all participants gave their consent after the study protocol had been explained to them .
387 ( article 25 ) dated 31 july 2006 about ethical trials in algeria , we obtained the required access authorizations to the concerned health facilities in order to accomplish our study protocol .
furthermore , we obtained informed written consent from all participants and their treating physicians after the study protocol had been explained to them .
il-1-31 and il-1-511 polymorphisms are single nucleotide changes in the promoter region , while the il-1rn polymorphism is a repetition of 86 bp in the second intron . the dna was extracted from 200 l whole blood samples , 20 mg of fresh gastric biopsy , or buccal swab in h. pylori transport medium using an extraction kit ( magazorbe genomic dna , promega , fitchburg , wisconsin , usa ) stored at 20c .
genotyping of the two polymorphisms ( il-1-31 and il-1-511 ) was performed using the restriction fragment length polymorphism method ( pcr - rflp ) ( table 1 ) ( 6 , 10 ) .
briefly , 2550 ng of dna was used in a volume of 25 l with 150 mm dntp , 6.25 pmol of each primer , 1 u of dna polymerase gotaq g2 hot start polymerase ( promega , fitchburg , wisconsin , usa ) , 5% dmso , 4 mm mgcl2 , and 5 l of 5x pcr buffer gotaq .
the amplified / digested fragments were visualized with the gelred nucleic acid gel stain , 10,000 x ( biotium , hayward , ca , usa ) in 2.5% agarose gel .
primers and conditions for analysis of the il-1 and il-1rn polymorphisms tm : melting temperature ; il-1-31 : cluster of gene encoding interleukin-1 in the promoter region of tata box at the 31 position ; il-1-511 : cluster of gene encoding interleukin-1 in the promoter region of tata box at the 511 position ; il-1rn : gene encoding the anti - inflammatory antagonist ( interleukin-1ra ) . variable numbers of tandem repeats ( vntr ) analysis in the second intron of the il-1rn gene was carried out by pcr .
the different alleles were identified by the size of the pcr fragments and encoded in a conventional manner ( 6 , 13 ) as follows : allele 1 ( four repetitions ) , allele 2 ( two repetitions ) , allele 3 ( five repetitions ) , allele 4 ( three repetitions ) , and allele 5 ( six repetitions ) . according to previous studies ( 6 , 13 ) , as described in table 1 , the il-1rn alleles were dichotomized into two categories : long genotype ( l:>three repetitions ; alleles 1 , 3 , 4 , 5 ) and short genotype ( 2 : two repetitions ; allele 2 ) .
data were processed and analyzed using spss 22.0 ( statistical package for the social sciences , ibm corporation ; chicago , il , august 2013 ) .
chi - square test was used to evaluate differences in the distribution of polymorphisms between different groups .
all allelic distributions were examined for the corresponding differences of their hardy - weinberg equilibrium .
the relative risk was evaluated by multivariate logistic regression based on categorical reference predictor models , adjusting for sex and age with a confidence interval ( ci ) of 95% and statistical significance set at p<0.05 .
allelic combinations of models of the three variants closely related polymorphisms of il-1 and il-1rn were analyzed .
the study of the haplotypes and linkage disequilibrium between loci ( estimated by r 
  and d ) was carried out via the web interface in the linkdos ( www.genepop.curtin.edu.au/linkc.html ) .
the mean age of all participants was 45.017.67 years , and ranged from 16 to 86 years .
in healthy individuals , the mean age was 35.812.72 years with a male - to - female ratio of 1:1 ( table 2 ) .
however , in patients with ag and/or gc , mean ages were 49.319.35 ( male - to - female ratio of 0.56:1 ) and 59.314.00 years ( male - to - female ratio of 2.27:1 ) , respectively .
comparison between groups according to gender ag : atrophic gastritis ; gc : gastric carcinoma ; hc : healthy controls comparison between the three groups using chi - square test .
the frequencies of the tested genotypes were in hardy - weinberg disequilibrium according to the following results .
linkage disequilibrium analysis between il-1-31 and il-1-511 for ag patients gave the following results : r=0.6804 , d=0.46294 ; moreover , analysis of linkage disequilibrium between il-1-31 and il-1-511 in gc patients : r=0.69357 , d=0.48 .
considering the whole studied population , il-1rn was positively correlated ( r=0.424 , r=0.180 ) with il-1-31 ( p=0.023 ) and with il-1-511 ( p=0.006 ) . in patients with gc , we noticed weak positive correlations ( r=0.377 , r=0.142 ) of il-1rn with il-1-31 ( p=0.550 ) and il-1-511 ( p=0.056 ) , respectively .
non - significant correlations ( r=0.454 , r=0.206 ) between il-1rn , on the one hand , and il-1-31 ( p=0.703 ) and il-1 -511 ( p=0.072 ) , on the other hand , were observed in patients with ag .
nevertheless , the correlations were non - significant in healthy subjects ( p=0.134 for il-1-31 and p=0.058 for il-1-511 ) .
as depicted in table 3 , the frequency of l / l wild - type pattern of il-1rn was 87.5% in controls compared to 62.5% in gc and 82.1% in ag patients .
the il-1rn2 allele rates , for either heterozygous or homozygous , were 37.5% in gc , 17.9% in ag , and 12.5% in controls .
distribution of the genotypes in the study population ag : atrophic gastritis ; gc : gastric carcinoma ; all : atrophic gastritis and gastric carcinoma ; hc : healthy controls ; il-1-31 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the -31 position ; il-1-511 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 511 position ; il-1rn : gene encoding for the anti - inflammatory antagonist ( interleukin-1ra ) ; l : long genotype ( more than three repetitions ; alleles 1 , 3 , 4 , 5 ) ; 2 : short genotype ( two repetitions ; allele 2 ) ; c or t : single nucleotide polymorphisms . regarding the il-1-511 ,
the proportions of c / c wild - type pattern were 56.3% in healthy subjects , 47.5% in gc , and 23.1% in ag patients .
individuals carrying the allele il-1-511 t are represented by 76.9% of ag , 52.5% of gc , and 43.8% of healthy controls .
our results show that 53.1% of healthy subjects , 35.9% of patients with ag , and 25.0% of patients with gc have the t / t wild - type pattern of il-1-31 . as illustrated in table 4 , we investigated major genotypes implicated as risk factors for having gc and ag compared to controls .
when multivariate logistic regression analysis was performed , our results showed that the c / c homozygous 4.614 ( 95% ci , 1.43714.817 ) and the c / t heterozygous 1.974 ( 95% ci , 0.5626.939 ) genotypes of the il-1-31 each indicated a strong contribution to gc .
likewise , the same genotypes have non - significant risk of having ag ( c / c : 2.429 [ 95% ci , 0.7697.673 ] and c / t : 1.455 [ 95% ci , 0.4025.260 ] ) .
however , the association between the two homozygous and heterozygous genotypes c / t and t / t of il-1 -511 was 4.286 ( 95% ci , 1.54411.898 ) times more at risk of having ag compared to controls .
regression analysis of the distribution of the genotypes in the study ag : atrophic gastritis ; gc : gastric carcinoma ; hc : healthy controls ; or : odds ratio .
gc * hc : regression analysis of combined gastric carcinoma and healthy controls , ag * hc : regression analysis of combined atrophic gastritis and healthy controls , ag * gc : regression analysis of combined atrophic gastritis and gastric carcinoma . il-1-31 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 31 position ; il-1-511 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 511 position ; il-1rn : gene encoding the anti - inflammatory antagonist ( interleukin-1ra ) ; l : long genotype ( more than three repetitions ; alleles 1 , 3 , 4 , 5 ) ; 2 : short genotype ( two repetitions ; allele 2 ) ; c or t : single nucleotide polymorphisms . concerning the heterozygous genotype l/2 of il-1rn , our findings show that the odds ratio of having gc was 4.200 ( 95% ci , 1.23014.337 ) . further analysis of combined genotype between il-1rn and il-1-31 ( table 5 ) and il-1rn and il-1-511 ( table 6 ) was performed using  test .
the association between il-1-31 and il-1rn was significant in ag patients ( p=0.028 ) and in controls ( p=0.021 ) .
furthermore , the relationship between il-1rn and il-1-511 was significant for the same groups ( ag : p=0.014 , and hc : p=0.015 ) .
distribution of genotypes among patient groups regression analysis of combined ( il-1rn vs. il-1-31 ) chi - square test .
ag : atrophic gastritis ; gc : gastric carcinoma ; hc : healthy controls ; il-1-31 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 31 position ; il-1-511 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 511 position ; il-1rn : gene encoding for the anti - inflammatory antagonist ( interleukin-1ra ) ; l : long genotype ( more than three repetitions ; alleles 1 , 3 , 4 , 5 ) ; 2 : short genotype ( two repetitions ; allele 2 ) ; c or t : single nucleotide polymorphisms .
distribution of genotype among patient groups regression analysis of combined ( il-1rn and il-1-511 ) chi - square test .
ag : atrophic gastritis ; gc : gastric carcinoma ; hc : healthy controls ; il-1-31 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 31 position ; il-1-511 : cluster in gene encoding interleukin-1 in the promoter region of tata box at the 511 position ; il-1rn : gene encoding for the anti - inflammatory antagonist ( interleukin-1ra ) ; l : long genotype ( more than three repetitions ; alleles 1 , 3 , 4 , 5 ) ; 2 : short genotype ( two repetitions ; allele 2 ) ; c or t : single nucleotide polymorphisms .
the paradigm of previous studies on gc with regard to different haplotypes of il-1rn and il-1 has been investigated since 2000 on scottish and polish populations ( 6 , 7 ) .
the genotype carriers of homozygous il-1-31c and il-1rn2 have a high risk of hypochlorhydria as a result of h. pylori infection with evidence for genetic
machado et al . ( 13 ) reported results on a portuguese population by studying the etiological role of il-1-511 t allele , il-1rn2 allele , and their association with an increased risk of developing intestinal gc .
until now , polymorphisms in genes of the pro - inflammatory cytokines il-1 and tnf- constitute an increased risk of developing gc in different gastric anatomical sites when the patients ethnicity is not considered ( 1719 ) .
several studies on the association between il-1 and the il-1rn genotype in asian and caucasian populations show that il-1-511 t is significantly associated with the risk of gc ( 20 ) .
other studies showed that il-1-511 t and il-1rn2 were associated with an increased risk of developing gc in caucasians .
similarly , a significantly increased risk was noticed when analyses showed positive associated with h. pylori infection with a moderate increase among hispanics , but not among asians ( 15 , 2123 ) .
to the best of our knowledge , only one study on the il-1 and il-1rn polymorphisms and their association with gastric cancer was performed in an arab population , namely in oman .
the authors found that the il-1rn2 polymorphism increases the risk of gc , but the il-1-31c allele was not associated with the risk of developing gc ( 24 ) . in africa , kimang'a studied the association of these polymorphisms with h. pylori infection with different pathologies of the gastric mucosa without considering gc .
he reported a significant effect of the il-1-511 t allele on gastritis , gastrointestinal reflux and gastric ulcers ( 25 ) . in this study
, we evaluated the association between il-1 and il-1rn vntr polymorphisms and the risk of ag and cg in northwestern algeria . according to globocan 2012 ,
gc is the fourth most common cancer ( both genders ) in algeria , with about 686 cases per year ( 26 ) .
our results show that the il-1-31c allele is associated with an increased risk of developing gc ( or=4.614 ; p=0.01 ) . the il-1rn2 heterozygous allele type is significantly associated with ag ( or=4.2 ; p=0.022 ) .
these findings are in concordance with results on caucasians but different from those of asian and arab populations in oman ( 24 , 27 ) .
considering the il-1-511 t allele , no significant effect on gc was noticed in the studied patients .
this result is in agreement with studies on japanese and korean populations ( 27 , 28 ) , but different from results from caucasian populations ( 7 , 13 ) .
individuals carrying the il-1-511 t allele have more risk of developing ag ( or=4.286 ; p=0.005 ) than gc .
contrariwise , in a recent study from mexico , individuals carrying il-1-511c and il-1-31 t alleles separately are more likely to develop ag and gastric ulcers ( 30 ) .
moreover , this work investigated the association between h. pylori infection and polymorphisms in only two genes , il-1 and il-1rn , without invoking other carcinogenic factors ; this could be considered as another limitation . in conclusion
, the presence of il-1 and il-1rn polymorphisms with h. pylori infection of the gastric mucosa contributes to the development of chronic ag and gcs in an algerian population .
individuals carrying the il-1-31c and il-1rn2 alleles are more likely to have an increased risk of developing gc . on the other hand
these results highlight the importance of ethnicity studies about the effect of il-1 and il-1rn polymorphisms associated with h. pylori infection on gastric carcinogenesis .
thus , large investigations needed to gain a proper understanding of gastric mucosa carcinogenesis in our region .
the authors have not received any funding or benefits from industry or elsewhere to conduct this study .
 backgroundinfection with helicobacter pylori is considered a potential risk of developing gastric cancer in association with contributing host genetic factor . il-1 and 
 il-1rn polymorphisms appear to maintain and promote helicobacter pylori infection and to stimulate neoplastic growth of the gastric mucosa.objective and methodsin order to elucidate the effect of these polymorphisms in combination with gastric cancer in a population from northwestern algeria , a case - control study was carried out on 79 patients infected with h. pylori with chronic atrophic gastritis and/or gastric carcinoma , and 32 subjects were recruited as case - control . 
 il-1-31 bi - allelic and il-1-511 bi - allelic polymorphisms and il-1rn penta - allelic were genotyped.resultsil-1-31c was associated with an increased risk of developing gastric carcinoma ( or=4.614 [ 1.4314.81 ] , p=0.01 ) . 
 however , il-1rn2 heterozygous allele type was significantly associated with chronic atrophic gastritis ( or=4.2 [ 1.233.61 ] , p=0.022 ) . 
 il-1-511 t was associated with an increased risk of development of chronic atrophic gastritis ( or=4.286 [ 1.5411.89 ] , p=0.005).conclusionil-1 and il-1rn polymorphisms associated with h. pylori infection contribute to the development of chronic atrophic gastritis and gastric carcinomas in an algerian population . 
 the alleles il-1-31c and il-1rn were associated with an increased risk of developing gastric carcinoma , and il-1-511 t with an increased risk of developing chronic atrophic gastritis with no significant association of developing gastric carcinoma . 
Methods
Patients
Analysis of IL-1
Statistical analysis
Results
Discussion
Conflict of interest and funding