comparison of dentine and bone in health and disease research on medical tissues in health and disease ( bone and osteoporosis ) is at least a magnitude more vivid than that in dentistry ( dentine and caries ) .
dental caries was the reason for the establishing the first dental school in baltimore , usa in the mid of 18th century .
this review article is an attempt to shed light on the role of enzymes during dentinogenesis and dentine steady state in health and caries taking models of enzyme functions from bones .
ectoderm covering the stomodeum of an embryo begins to proliferate , giving rise to dental laminae , which occurs in humans during the sixth week in utero .
reciprocal interactions between the ectoderm and mesoderm layers lead to placode formation , some of which develop into tooth germs , containing an enamel organ , dental papilla , and dental follicle .
therefore , teeth are made of ectodermal ( enamel organ ) and ecto - mesenchymal components , containing neural crest - derived cells , which display multipotent capabilities .
dental pulp is externally separated from dentine by odontoblasts and by hhls subodontoblastic cells which are preodontoblasts .
adjacent to this layer , the pulp is rich in collagen fibbers and poor in cells .
then a more internal layer contains progenitor cells and undifferentiated cells , some of which are considered stem cells .
the innermost layer is the core of the dental pulp and comprises the vascular plexus and nerves but some nerve branches and capillary vessels reach the odontoblast layer .
the final differentiation of tooth - forming cells , as well as matrix secretion and mineralization , takes place in the bell - stage .
the junction between the dental papilla and inner enamel epithelium determines the final crown shape and size of a tooth .
odontoblasts secrete a collagenous matrix , called predentine and at the onset mantle dentine , later term being dentine , which is then mineralized during primary dentinogenesis .
primary , secondary and tertiary dentinogenesis primary dentinogenesis in humans is completed when the tooth becomes functional and contacts are established between maxillary and mandibular teeth by late adolescence or early adulthood when it turns into secondary dentinogenesis .
nonproliferative odontoblasts reduce the speed of deposition or , may enter the quiescent state and may remain quiescent during their lifetime .
therefore , the term  post - mitotic  is often used to refer to both quiescent and senescent odontoblasts .
when primary dentinogenesis turns into secondary but nevertheless , these cells maintain the same denomination .
odontoblast is a nomenclature which has been established by waldeyer as early as in 1885 .
cyte  is employed in mineralized tissues to designate the quiescent stage of a cell that previously underwent an active secretory stage and then remained surrounded by the mineralized matrix like osteocytes and cementocytes .
however , in living cells , only the speed of deposition varies between the blast- and cyte- phases .
one exception exists : the cells of the growth plates of long bones proliferate rapidly and are termed chondrocytes , until they undergo apoptosis at the latest when the youngster turns into an adult . the suffix 
cyte  is now used in mitotic cells . in puberty , both the growth of humans and the elongation of their tooth roots will halt . while the dividing chondrocytes inside the mineralized matrix of bones go through apoptosis and
disappear , the odontoblasts which become quiescent are not renamed odontocytes . the present nomenclature may change in the future : recently larmas and sndor   suggested that odontoblasts during secondary dentinogenesis should be renamed as 
most mammalian teeth cease their elongation at the cessation of root formation . in some teeth , however , transdifferentiation from odontoblasts to odontocytes does not happen , which would explain why some mammalian teeth ( elephant tusks and rodent incisors ) are ever - growing
. during tertiary dentinogenesis , which is not physiological and occurs in response to a local stimulus , the situation changes again : the resting odontoblasts / odontocytes may return to an activated stage ( forming reactionary dentine ) and/or die out and may be replaced by other cells of pulpal origin . in this case
tertiary dentine is then called reparative dentine ( table 1 ) . 
 stages of dentinogenesis , cells , and enzymes involved in it 
 
do adult odontoblasts transdifferentiate into  odontocytes  in teeth with closed root apices , or are they then  secondary odontoblasts  and after injury  tertiary odontoblasts  or 
this textbook nomenclature is based on the original observations of couve   in growing teeth in humans and confirmed recently also in adult teeth .
since there were no interventions or randomized controlled trials involving the role of enzymes during dentinogenesis and dentin steady state identified in a preliminary literature search , the preferred reporting items for systematic reviews and metaanalysis ( prisma ) statement   or cochrane handbook   were not used in this review .
the keywords used for the searches ( in august , 2014 ) were  enzymes  and  odontoblasts  .
only articles written in english were included and their number restricted to enzymes , dentinogenesis and caries : ultimately a manual search of english language dental anatomy and textbooks was performed . using keywords 
enzymes  and  odontoblasts  pubmed hits were 598 while scopus hits were 371 . using  enzymes  and  dental caries 
enzymes  and  dentine caries  in pubmed gave 179 hits and scopus had 100 hits . 
enzymes  and  enamel caries  in pubmed resulted in 131 hits while scopus gave 81 hits .
 enzymes  and  dentinogenesis  in pubmed resulted in 183 hits while with scopus there were 91 hits . using key words 
enzymes  and  primary dentinogenesis  there were 18 hits in pubmed and in scopus there were 8 .  enzymes  and  secondary dentinogenesis  gave 20 hits in pubmed and 8 hits in scopus . 
enzymes  and  tertiary dentinogenesis  gave only 6 hits in pubmed and 2 in scopus .
scopus gave a total of 100 hits for  enzymes  and  dentine caries  and those were reported as follows : larmas 14 , mkinen 9 , and tjderhane 8 , all authors being from the same research group .
when the english language search was restricted to  enzymes  ,  dentinogenesis  and  caries  then only 3 - 4 hits were produced by pubmed and scopus respectively .
cellular events in the early differentiation phases the final differentiation of the tooth - forming cells from embryonic progenitor / stem cells take place in the bell stage of tooth development .
the columnar cells of the enamel organ adjacent to the dental papilla are known as the inner enamel epithelium , which are differentiated from stem cells of inner enamel epithelium , or ameloblasts , as they move outwards or upwards .
ectomesodermal stem cells differentiate into odontoblasts and secrete a collagenous matrix which is then mineralized into extracellular dentine .
first , the enamel - forming ameloblasts secrete an organic matrix that is then mineralized .
thereafter , during a distinct stage called enamel maturation , a sudden and massive influx of mineral ions takes place .
the maturation stage initiates in the cusps while the secretory phase continues in the more cervical parts of the enamel organ .
enzymes in undifferentiated dental cells in order to reach an integrated picture of cell metabolism at the onset of tooth ontogeny , several histochemical studies have been performed on enzymes in cells and structures in experimental animals .
enzymes participating in cell metabolism are normally demonstrable at the stage of active differentiation , but also in differentiated tooth forming cells .
however , it should be considered that quantification of enzyme activity by histochemical means is impossible because changes in enzyme activity may represent a direct change in the number of enzyme molecules , alterations in the physical state of the enzyme molecules or the effects of various co - factors in addition to technical assay problems .
histochemical studies of differentiating , developing and forming human teeth and supporting structures have also been performed .
several arylaminopeptidases and glucosidases were demonstrated in the basal cell layer of stomodeum , in the subjacent mesoderm , in the outer and inner enamel epithelium , in the dental papilla and lamina , and in the primordium of permanent tooth .
no arylaminopeptidase activity could be observed in ameloblasts , odontoblasts , enamel , dentine , stellate reticulum , and pulp , and the inner enamel epithelium also lost its activity during the stage of apposition .
all structures ( except enamel and dentine ) of the human tooth germ revealed dehydrogenases including lactate ( ldh ) , succinate ( sdh ) , glutamate ( gdh ) , and nadh2 activities in different patterns .
ldh activities decreased during the development in the epithelium and the subjacent mesoderm up to the stage of apposition , when ameloblasts and odontoblasts revealed intense ldh , weak sdh activity , and strong nadh2 - diaphorase activity .
these observations suggested that anaerobic glycolysis as an energy source dominated in mineralizing cells at the onset of enamel and dentine formation .
histochemical analyses are essential for understanding the localization of molecules , and biochemical studies are needed for the demonstration of real changes in enzyme activity . in experimental mice , a considerable rise in the membrane - bound enzyme , non - specific alkaline phosphatase ( apase ) activity in the mesenchymal part of tooth germs between the 18 prenatal day and the 1 day was seen .
that time corresponds to the onset of mantle dentine secretion by odontoblasts in mice . because the protein content of the samples also increased during this 2-day period
this stimulation further increased up to the 7 day , after which the protein content dramatically decreased in 4 days and the apase activity in 10 days , indicating that the stimulation period of apase activity in mesenchyme had ended .
the secretion of enamel in mice molars starts between the 1 and 2 postnatal days , at which time the increase in epithelial apase becomes pronounced and continues up to the 7 day .
the observed changes in apase activity in both ectodermal and ectomesenchymal parts of the tooth germs could be due to changes in the number of cells associated with the synthesis and/or activation of apase molecules .
epithelial and mesenchymal parts of teeth could not be isolated at day 11 , when the mice molars had not emerged , but their crown development was complete .
then a significant decrease in the apase activity was seen in the mesenchyme . because the mineralization of dentine matrices closely follows the secretory phase , the role of apase in either secretion or mineralization can not be distinguished .
the developmental stage in the cusp region is more advanced than in the cervical part .
the activity of matrix metalloproteinases ( mmps ) , a class of ca / zn - dependent endopeptidases that remain trapped within the mineralized dentine matrix , is regulated by members of the timp ( tissue inhibitors of metalloproteinase ) family .
the expression of gelatinase a ( mmp-2 ) and gelatinase b ( mmp-9 ) as well as timp-1 , -2 and -3 genes during different stages of mouse tooth development was analyzed .
gene expression was generally found in mesenchymal tissues except for timp-3 , which also was found in dental epithelial cells .
gelatinase b and timp-1 and timp-3 expression showed clear association with epithelial morphogenesis and was restricted to the mesenchyme at the tip of the growing tooth bud .
gelatinase a and timp-1 showed transient expression in secretory odontoblasts at the time of basement membrane degradation , while timp-2 expression continued throughout the dental papilla .
cellular events in primary dentinogenesis during primary dentin formation , odontoblasts retreat from the mineralization front , leaving behind cellular processes that remain trapped in dentinal tubules .
for example , estrogen deficiency , induced by ovariectomy , caused enhanced dentine formation in adult rats , suggesting that estrogen receptors may be present also in dental tissues .
odontoblasts are reported to reveal the expression of mineralization - related genes , including dentine sialophosphoprotein and dentine matrix protein-1 [ 18 - 20 ] .
odontoblasts are dividing in the elongating permanent tooth until the closure of the root apex .
after apical closure old odontoblasts or odontocytes continue to maintain their vital functions during secondary dentinogenesis , which maintains the mechano - modulatory role in adult dentine with a very low rate of dentine formation but contrary to bone , without dentine resorption .
the areas of a developed caries lesion and formed dentin under the lesion during the cariogenic challenge positively correlated in all molars up to 7 weeks after weaning of the rat pup .
then a dramatic drop in both the rate of caries progression and that of dentine formation was seen when compared to adult rats .
some genetic disorders may be linked to the stages of odontoblast differentiation such as dentinogenesis imperfecta in which teeth are blue - brown colour with pulp obliteration or large pulp chambers .
dentinogenesis imperfecta type ii and type iii usually occur in people without other inherited disorders .
this disturbance was connected to stages of odontoblast differentiation : if primary odontoblasts turn into odontocytes , primary dentinogenesis does not progress to secondary dentinogenesis causing type i and ii dentinogenesis imperfecta meaning that odontoblasts are evergrowing .
another genetic disorder , hypophosphataemic vitamin d - resistant rickets , is characterized by pulpal horns which extend to the enamel and by globular circumpulpal dentine in humans .
the globules were normally mineralized , whereas the interspaces between the globules were not mineralized at all .
mantle dentine below the enamel - dentine border was not globular until a width of 0.2 mm toward the pulp was reached .
evidently , ameloblasts or hertwigs epithelial cells resulted in the non - globular region by odontoblasts under the dentino - enamel and cementum - dentine junction .
recent studies have identified a number of molecules with phosphaturic activities , including fibroblast growth factor-23 ( fgf-23 ) and matrix extracellular phosphoglycoprotein .
fgf-23 has been implicated in various human diseases , including autosomal dominant hypophosphatemic rickets   and x - linked hypophosphatemia .
fgf-23 is mostly expressed in bone and connective tissue and prior to 2000 was known in the scientific community as phosphatonin .
enzymes in primary dentinogenesis a series of biochemical enzyme assays from samples taken from extracted human teeth was also performed .
samples of drilled dentine powder were taken from ( 1 ) crown enamel dentine , ( 2 ) crown dentine , ( 3 ) crown odontoblastic ( circumpulpal ) dentine , ( 4 ) root odontoblastic dentine , ( 5 ) apical odontoblastic dentine as well as soft carious dentine , from developing teeth and fully - formed teeth .
apase activity was highest in all circumpulpal samples from emerged but still developing sound human teeth   and a dramatic drop in the activity was seen in these samples .
this drop was so distinct that apase activity per protein or per wet weight of the sample was used as an indicator of the closure of the apex in clinically borderline cases : high activity represented open apex , low activity indicated the closure .
cell function in secondary dentinogenesis in the adult human teeth the cell division of old odontoblasts or odontocytes is zero and their secretory activity is limited , resulting in secondary dentine formation which is very slow when compared to that of primary dentine apposition .
odontoblasts begin as polygonal cells that leave behind processes in the dentine matrix with numerous branches .
compared early and late odontoblasts with osteoblasts and osteocytes , using two osteocyte markers , dmp1 , an acidic extracellular matrix protein , and e11/gp38 , a transmembrane glycoprotein   decreased mineral deposition and lower expression of e11 and other osteocyte markers such as dmp1 . a huge difference between odontoblasts and osteocytes exists since the odontoblasts / cytes are not completely embedded in mineralized matrix like osteocytes .
osteocytes communicate between each other and with osteoblasts / bone lining cells by gap junctions found in their plasma membrane in the end their processes inside the canaliculi , which does not occur in odontoblasts in the same manner .
however , a drop in the apase activity was used as an indicator of the closure of the tooth root apex even in the crown area : high activity represented open apex , low activity indicated the closure   and therefore , the existence of a communication system between odontoblasts is supported since some system exists linking odontoblasts in the apex and crown areas .
the remarkable similarities in morphology and the three - dimensional canalicular systems in both odontoblasts and osteocytes , together with a very similar expression pattern of two osteocyte markers , dmp1 and e-11 , in the processes of odontoblasts and dendrites of osteocytes , supported the differentiation pattern of osteoblasts into osteocytes in bone .
terminally differentiated adult odontoblasts , or odontocytes , were found to develop an autophagic - lysosomal system organized mainly by large autophagic vacuoles that are acid - phosphatase - positive to various degrees .
these vacuoles expressed the autophagosomal and lysosomal markers lc3 and lamp2 in an age - related pattern .
recently , couve et al .   confirmed these observations of an autophagic - lysosomal system and additionally found mitochondrial autophagy and lipofuscin accumulation in aging odontoblasts .
enzymes in secondary dentinogenesis in sound and carious human teeth histochemical methods of the localization of many enzymes in sound and carious human teeth in different developmental or pathological stages were conducted some decades ago ( figure 1 ) .
hydrolysis of numerous naphthol derivatives was histochemically detected in the dental plaque on advanced carious lesions and in the tubules of soft carious dentine , indicating the occurrence of arylaminopeptidases , carboxylic ester hydrolases , acid phosphatase , phosphoamidases , pyrophosphatases , glycosidases , and arylsulphatase   in dentinal tubules of soft carious dentine ( figure 1 ) .
dental plaque and dentinal tubules of carious dentine also revealed ldh , glucose-6-phosphate , and sdh activity ( figure 1e , 1f ) , ldh activity was demonstrable even in the area where no microorganisms ( figure 1f ) were seen under the carious lesion .
examples of the presence of certain hydrolases and oxido - reductases in soft carious dentine of extracted human molar teeth . a = arylaminopeptidase activity in the dentinal tubules of soft carious dentin .
b = higher magnification ( x165 ) of arylaminopeptidase on the borderland between hard ( under ) and soft carious dentine ( above ) . c = glocose-6-phosphate - dehyrogenase in soft carious dentine .
f = higher magnification ( x451 ) of lactate dehydrogenase activity on the borderland between hard ( fractured ) and soft carious dentine revealing enzyme activity in dentinal tubules , ahead microorganims .
the picture is reconstructed from the original slides published by larmas et al . in acta odontol
scand 26 : 127 - 136 , 1968 ( a and b ) and larmas in arch oral biol .
acid phosphatase , glucuronidase , and glycosidase substrates were observed to be hydrolyzed in the odontoblastic layer and the predentine , which area also revealed high apase and occasional succinate dehydrogenase activity .
no activity of any enzyme was demonstrated in hard dentine of sound or carious teeth .
quantitative enzyme activity determinations were also conducted with samples from both soft carious dentine , and from hard , pigmented carious dentine in fully - formed sound and enamel carious lesions , from teeth with dentine caries , teeth with pulpitis , and fully - formed teeth with necrotic pulps .
observations confirmed that apase was activated in peripulpal odontoblasts when caries was in enamel only , further activation was seen when the process reached dentine , which activation was also seen in the peripulpal area of the apex .
these observations were thought to support the view that apase in the entire circumpulpal odontoblast layer reacts as a network of cells in response to caries .
biochemical analysis of the rate of hydrolysis of arylaminopeptidase substrates revealed that enzyme activity was at least ten times higher in soft carious dentine than in non - carious coronal dentine .
approximately three times as much arylaminopeptidase activity was seen in carious lesions from necrotic teeth than lesions from vital teeth which indicated that these teeth harboured different types of microbes .
the occurrence of aminotransferases both in sound and carious human dentine was reported , too   indicating degradation of amino acids during the carious process .
changes of these enzyme activities indicated that both microbial metabolism and odontoblastic response mediated the process of progression of caries in dentine .
cell function in tertiary dentinogenesis the most common feature of pulp repair in injury is the formation of tertiary dentine , a subgroup of which is reactionary dentine .
the latter generally follows a mild injury ( for example carious lesions of only moderate progression ) , which odontoblasts survive after the stimuli .
the regulation of odontoblast / cyte activity is clearly central to tooth response against injury .
more intense injury that later leads to odontoblast death is followed by the formation of reparative dentine
. then more complex defence and healing responses occur , with recruitment of stem or progenitor cells from pulpal origin , first reported by gronthos et al . , their differentiation to odontoblast - like cells , and subsequent up - regulation of secretory activity and tissue mineralization which is called reparative dentinogenesis .
investigation of genes and/or signaling pathways which might represent targets for the switch from secondary to tertiary dentinogenesis is important .
endopeptidases in mature human odontoblasts and pulp tissue a couple of decades ago it was not possible to reveal any proteolytic enzyme activity in hard , fully - formed human dentine either by histochemical or biochemical enzyme assays but soft carious dentine revealed peptidase activity   and endopeptidases were also demonstrable by biochemical means . therefore , with the development of more sophisticated enzyme activity assays the focus was directed to mmps .
activation of the proteolytic enzymes may be a critical step that leads to dentine extracellular matrix breakdown , especially in the remodelling of predentine to allow dentine formation to occur .
first the presence of gelatinases ( mmp-2 , -9 ) was demonstrated   as well as collagenase-2 ( mmp-8 )   in mature healthy dentine .
more detailed analysis demonstrated that mmp-14 ( membrane type mmp , mt1-mmp ) mrna was expressed by native and cultured mature human odontoblasts and pulp tissue .
western blot analysis of human odontoblasts and pulp tissue detected pro- and active forms of mt1-mmp , and smaller truncated mt1-mmp forms .
bmp-2 down - regulated mt1-mmp expression in odontoblasts and pulp tissue , while tgf-1 , alone or with bmp-2 , decreased mt1-mmp mrna levels only slightly .
it was also demonstrated that mt1-mmp was capable of converting prommp-20 into a form corresponding to the active mmp-20 . in conclusion
, observations demonstrated the expression and differential regulation of mt1-mmp in human dentine - pulp complex cells , and the activation of mmp-20 by mt1-mmp .
some osteocyte markers , like bmp-2 , down - regulated mmp-14 expression in mature odontoblasts while tgf-1 alone or with bmp-2 decreased mmp-14 mrna levels slightly .
thus , old odontoblasts / cytes share similar features with osteocytes with regard to the mmp family , which may safeguard osteoblasts from apoptosis during transdifferentiation into osteocytes .
studied the presence and localization of mmp-20 in mature human teeth in health and disease . in immunohistochemistry ,
mmp-20-positive staining was observed most intensively in the radicular odontoblastic layer and also in dilated dentinal tubuli of caries lesions . by western
blotting , mmp-20 was detected in odontoblasts and pulp tissue of both sound and carious teeth , in dentinal fluid and dentine of sound teeth , but not in soft carious dentine .
mmp-20 produced during primary dentinogenesis was synthesized and secreted by ameloblasts and incorporated into dentine outer layer and may be released during caries progression .
the main cellular source of mmp-20 in the dentine - pulp complex was the odontoblasts , which secreted mmp-20 into the dentinal fluid .
the fei - sem analysis revealed positive immunolabelling patterns for mmp-3 , predominantly localized on the intertubular collagen fibrillar network showing mmp-3 directly or indirectly bound to the collagen fibrils .
in carious dentine , cysteine cathepsin activity increased with increasing depth and age in chronic lesions , but decreased with age in active lesions .
recently it was also reported that the abundance of cysteine cathepsins and mmps was markedly higher in caries - affected than in intact dentine .
cysteine cathepsin - b exhibited the highest percentage of co - localization with collagen , followed by mmp-9 , mmp-2 , and cysteine cathepsin - k .
the high expression of cysteine cathepsins and mmps in caries - affected teeth indicated that those host - derived enzymes were intensely involved with caries progression .
histochemical localization of apase in developing teeth with advanced dentinal caries when the carious process was so progressive that it reached the dental pulp in a developing human molar tooth , the histochemical distribution of apase was interesting : enzyme activity disappeared from the odontoblasts of the pulp exposure area , where cells evidently were not vital any more in these growing teeth .
all other odontoblasts around the pulp chamber exhibited strong apase activity , especially in the cells of the open root apices , but also periodontal structures on the root surface around the apex areas , exhibited high activity ( figure 2 ) .
a = a microradiograph of a developing human molar tooth with an advanced carious lesion .
b = localization of alkaline phosphatase activity in the odontoblasts , predentine , cementum , in remaining parts of the periodontal ligament , and some pulpal cells .
c = higher magnification of the localization of alkaline phosphatase activity in pulpal cells under the advanced carious lesion with pulp perforation and pulpal inflammation .
note the disappearance of enzyme activity in odontoblasts in the site of carious exposure and appearance in dentinal tubules in soft carious dentin .
d = higher magnification of the same area of pulp horn showing the presence of arylaminopeptidase activity in the fibroblasts / progenitor / stem cells or inflammatory cells in the inflamed pulp horn .
substrate : n - l - arginyl-2-naphthylamine , fast blue b , ph , 6.8 .
the picture is originally published in the anatomical record : advances in integrative anatomy and evolutionary biology , 296 , p. 565
, 2013 , and the reproduction granted by wiley/ the copyright clearence center inc , license number 3213520064351 . in the inflamed dental pulp , under the pulp perforation area ,
high apase activity was present in pulp fibroblasts or progenitor / stem cells around the inflamed area , where enzyme activity encircled the inflamed pulp horn area ( figure 2c ) .
the presence of pulpal inflammation was indicated by an elevated arylaminopeptidase activity in the cells inside the inflamed pulp horn ( figure 2d ) .
tooth - ache was the cause of the extraction of that tooth and established the presence of inflammation and the histochemical findings .
it seems that apase in human dental pulp was activated by some signalling pathway either in the pulp fibroblasts or in some progenitor pulp cells or in pulp stem cells   under the inflamed pulp horn in a developing and elongating ( growing ) tooth or else , by some circulating progenitor / stem cells .
what seems certain is that no apase activity occurred in the odontoblasts in the pulp exposure area , but odontoblasts in the crown or root area exhibited enzyme activity in the developing tooth , whereas pulp fibroblasts normally were without activity . in the advanced stage of carious injury , odontoblasts were non - vital and unable to produce any reactionary dentine or apase
. it still remains unsolved whether pulp fibroblasts or progenitor / stem cells are capable of producing some reparative dentine , for example in the form of pulp stones .
the enzyme spectrum in differentiating and differentiated odontoblasts and in soft carious dentine is broad .
evidently most of the enzymes in carious tissue originate from microorganisms or saliva , which have penetrated into dentine .
whether these enzymes are catabolic for dentine or meant to maintain the vital functions of the microbes remains unsolved .
the catabolic role of mmp enzymes was supported by the findings that dentine mmps may participate in the destruction of dentine collagen following demineralization by bacterial acids
the presence of lactate dehydrogenase deep in the carious lesion suggests that the production of lactic acid is possible there and enables the dissolution of the dentine wall perpendicular to dentinal tubules and thereby their dilation , which explains the undermining appearance found in carious lesions .
the localization and activation of apase in odontoblasts and pulpal fibroblasts or progenitor cells point to the role of apase in cell differentiation during dentine formation .
stem cell - based tooth regeneration is a promising approach to solving the problems of dentine caries and human skeletal muscle stem cells could also differentiate into odontoblasts but required an extracellular matrix scaffold and changes in their integrin profile that was evidenced by appearance of odontoblastic phenotypes .
though dental studies are scanty when compared to bone , the active role of large spectrum of enzymes in healthy and carious dentine was given support .
 abstractobjectivessearch in pubmed with keywords  enzymes , dentinogenesis , and dental caries 
  revealed only 4 items , but when combined with  enzymes , osteogenesis , and osteoporosis  as high as 404 items resulted . 
 dental caries was associated with an order of magnitude fewer studies than the chronic bone disease , osteoporosis . 
 this observation motivated this review.material and methodsa comprehensive review of the available literature on role of enzymes in dentinogenesis and dental caries was undertaken using medline ( pubmed ) and scopus . 
 keywords for the search were : enzymes and odontoblasts , enzymes and different forms of dentinogenesis as well as dental caries.resultssearch revealed studies which described odontoblasts harbouring numerous enzymes ( hydrolases , including metalloproteinases , transaminases and dehydrogenases ) during primary dentinogenesis . 
 alkaline phosphatase activity sharply decreased when odontoblasts turned into quiescent odontoblasts . 
 tertiary dentinogenesis was characterized first by reactionary dentine formation when alkaline phosphatase was highly reactivated . 
 then later some of these odontoblasts may die out and be replaced by other progenitor cells of pulpal origin . 
 this tertiary dentine was called reparative dentine . 
 pulpal progenitor / stem cells revealed alkaline phosphatase activity in areas encircling inflamed pulp sections . 
 soft carious dentine revealed high hydrolase , transaminase and dehyrogenase activities that may have originated from invading microbes , saliva or were endogenous . 
 proteolytic activity was especially demonstrable using histochemical and biochemical means . 
 specifically , matrix metalloproteases may have originated partly from activated proenzymes of host origin.conclusionsthough dental studies are scanty when compared to bone , the active role of large spectrum of enzymes in healthy and carious dentine was given support . 
INTRODUCTION
MATERIAL AND METHODS
RESULTS
DISCUSSION
CONCLUSIONS
ACKNOWLEDGMENTS AND DISCLOSURE STATEMENTS