several species of bacteria have been linked to chronic infections of colon and have been shown to increase the risk of colon cancer .
streptococcus bovis has been evaluated as one of the possible etiologic agents for colorectal cancer . 

s. bovis is a gram - positive bacterium , which is considered as a normal inhabitant of the human gastrointestinal tract but is less frequently present than other streptococcus species .
whatever , s. bovis has been shown to be an increased cause of endocarditis and bacteraemia and found to be   associated with gastrointestinal diseases [ 36 ] or with the colorectal cancer [ 710 ] .
although a number of bacteria have been associated with cancer , their possible role in carcinogenesis is unclear . in some cases
helicobacter pylori may cause stomach cancer [ 11 , 12 ] since animal models have demonstrated koch 's third and fourth postulates for the role of h. pylori in the causation of stomach cancer .
moreover , it has been found also that salmonella typhi is associated with gallbladder cancer   and escherichia coli with crohn 's disease as well as colon cancer [ 15 , 16 ] .  
mccoy and mason first reported a case of the association between streptococcal endocarditis and colon cancer in 1951 .
many studies have examined the presence of s. bovis in stool samples obtained from patients with colorectal cancer to find a relationship between this bacterium and the risk of colorectal cancer .   in 1977 , klein et al . found a significantly strong association of s. bovis in stool samples of patients with colon cancer compared with healthy controls and patients with nonmalignant gastrointestinal disease .
in contrast , other studies did not find any significant association between faecal s. bovis and human colorectal cancer [ 2124 ] .
the aims of the current study were to isolate s. bovis from stool specimens collected from patients with malignant and non - malignant gastrointestinal diseases and from a healthy group to compare the association of the bacterium in malignant and non - malignant gastrointestinal diseases and to determine the susceptibility of the isolated strains to different antimicrobial agents .
stool specimens were collected in sterile containers before the initiation of therapy from inpatients with malignant and non - malignant gastrointestinal diseases in ibn - sina specialized hospital , khartoum , sudan .
the stool samples were from 28 inpatients with malignant gastrointestinal diseases ( table 1 ) , 27 inpatients with non - malignant gastrointestinal diseases ( table 2 ) , and 50 controls .
20 out of the 50 controls were outpatients who came to the gastric intestinal tract clinic suffering from the gastrointestinal tract problems and the medical examination and laboratory tests showed that they did not have any type of cancer . the inpatients and the 20 controls were working in agriculture and animal husbandry and aged between 25 and 65 years .
5% of each inpatients and controls aged from 25 to 50 years and 95% aged from 51 to 65 years .
30 out of the 50 controls were apparently - healthy farmers aged 5086 years and the mean age was 64  10 years .
the stool samples were collected during the medical survey for infectious and tropical diseases organized by university of medical sciences and technology in sudan from gezira agriculture scheme with a population of 40 thousand people .
the stool samples were cultured on macconkey agar plates ( himedia limited , bombay ) and incubated aerobically at 37c overnight .
antimicrobial susceptibility to penicillin and trimethoprim - sulfamethoxazole was carried out on the isolated strains by disk diffusion method on mueller - hinton agar plates ( himedia limited , bombay ) .
sensitivity of bacteria to each antibiotic was carried out by measuring the diameter of inhibition of bacterial growth around the disc .
a diameter of 26 mm was considered as the cut - off point for sensitivity for the penicillin and a diameter of 24 mm for the trimethoprim - sulfamethoxazole according to national committee for clinical laboratory standard ( nccls ) method . 

 
					
					  test was used for significant prevalence of s. bovis in stool specimens from patients with malignant or with non - malignant gastrointestinal diseases .
the statistical analysis was also performed to compare between presence of s. bovis in stool specimens from patients with noncolonic cancer and with non - malignant gastrointestinal diseases .
stool specimens were collected from fifty - five patients with malignant   and non - malignant gastrointestinal diseases and from fifty persons as control group .
the specimens were examined to the prevalence of streptococcus bovis strains and their susceptibility to the used antibiotics .
all the isolated s. bovis strains were found to be sensitive to penicillin and trimethoprim - sulfamethoxazole with diameter of inhibition from 26 to 32  mm and from 24 to 32  mm , respectively .
s. bovis was not detected in the control group but it was detected in the inpatients aged 5165 years .
bacteriological analysis of stool specimens from the patients with malignant gastrointestinal diseases showed that out of 28 specimens s. bovis was isolated from 10 patients .
six positive were from patients with carcinoma of the stomach , and one positive was from each patient with carcinoma of rectum , pancreas , colon , and liver .
the prevalence of s. bovis in stool from the patients with carcinoma in stomach , rectum , pancreas , colon , polyps , and hepatocytes was 55% , 25% , 14% , 50% , 0% , and 50% , respectively ( table 3 ) .
analysis of stool specimens from the patients with non - malignant gastrointestinal diseases showed that out of 27 specimens s. bovis was isolated from 5 patients .
two positive from patients with liver cirrhosis , two positive from patients with obstructive jaundice , and one from patient with portal hypertension were found .
the prevalence of s. bovis in stool from the patients with crohn 's disease , liver cirrhosis , obstructive jaundice , portal hypertension , and viral hepatitis was 0% , 28% , 33% , 14% , and 0% , respectively ( table 4 ) . 
					
					
from analysing the prevalence of s. bovis in stool from the patients with malignant and non - malignant gastrointestinal diseases and healthy controls was 36% , 18% , and 0% , respectively ( figure 1 ) .
the statistical analysis showed that the prevalence of s. bovis in stool specimens from patients with malignant or with non - malignant gastrointestinal diseases was statistically significant ( p value of  test was 0.02 ) .
colorectal cancer is the fourth most common cancer among men and third most common among women worldwide .
a large number of previous studies point to association of s. bovis with gastrointestinal diseases [ 5 , 6 ] and cancer of the human colon .
the current study finds a correlative relationship between existence of s. bovis and malignant gastrointestinal diseases since prevalence of the bacterium in stool from the patients with carcinoma in stomach , rectum , colon , and hepatocytes was 55% , 25% , 50% , and 50% , respectively . in comparison ,
the prevalence of s. bovis in stool from the patients with crohn 's disease , liver cirrhosis , obstructive jaundice , portal hypertension , and viral hepatitis was 0% , 28% , 33% , 14% , and 0% , respectively .   
				 from analysis the prevalence of s. bovis in stool from the patients with malignant and non - malignant gastrointestinal diseases and healthy controls was 36% , 18% , and 0% , respectively .
the  test statistical analysis showed that the prevalence of s. bovis in stool specimens from patients with malignant or with non - malignant gastrointestinal diseases was statistically significant ( p = 0.02 ) .
the current findings confirm the previous data that correlated the association of s. bovis with colorectal cancer specially klein study 1977   and the later studies [ 1820 ] .
in contrast to the literature regarding an association with colorectal cancer , less is written about associations of s. bovis with other gastrointestinal diseases or with other cancers except klein study   1977   but klein 1987 reported also the lack of association of s. bovis with noncolonic gastrointestinal carcinoma . however , alazmi et al . , 2006 found that s. bovis bacteremia in adults was frequently associated with hepatic dysfunction , and zarkin et al .
, postulated a triad of s. bovis bacteremia , liver disease , and colonic pathology whereby the liver disease might account for the increased fecal carriage , entry to the portal venous system , or passage from the portal to systemic circulation of s.   bovis . in this context ,
our findings add more information about the association of s. bovis   with   non - malignant gastrointestinal diseases and with non - colonic cancer : since the current study isolated s. bovis from the stool of   patients with liver cirrhosis , obstructive jaundice , and portal hypertension ( table 4 ) and the bacterium was isolated from stool of   patients with carcinoma in stomach , rectum , and hepatocytes ( table 3 ) ,   the association of s. bovis   with   non - malignant gastrointestinal diseases or with noncolonic cancer was not significantly different by    test ( p > 0.05 ) . however , klein 1977 isolated s. bovis from stool of patients with inflammatory bowel disease , other gastrointestinal disorders and noncolonic neoplasms   and he found that presence of s. bovis in stool of patients with non - colonic cancer was not significantly different from that in controls .
tjalsma and colleagues detected immune reactions against s. bovis antigens in sera of 11 out of 12 colon cancer patients and in 3 out of 4 patients with colon polyps .
they found that one of the diagnostic antigens represents a surface - exposed heparin - binding protein that , according to their speculation , might be involved in the attachment of s. bovis to tumor cells . they claim that profiling of the humoral immune response against s. bovis infections may represent a promising diagnostic tool in early detection of human colon cancer .
however , research has not yet determined if s. bovis is a causative agent of colon cancer or if preexisting cancer makes the lumen of the large intestine more hospitable to s. bovis outgrowth .
previous findings ( reviewed in ) suggest an active role of s. bovis in the promotion of intestinal carcinogenesis when adult rats were treated with azoxymethane for 2 weeks and subsequently received injections with either s. bovis bacteria or wall - extracted antigens twice weekly .
the authors observed progression of preneoplastic lesions , enhanced expression of proliferation markers , and increased production of interleukin-8 in the colonic mucosa in these rats .
the same group used a partially purified s. bovis s300 fraction representing 12 different proteins and triggered the synthesis of proinflammatory proteins ( human interleukin-8 and prostaglandin e2 ) , correlated with the in vitro overexpression of cyclooxygenase-2 in human colon carcinoma cells and in rat colonic mucosa .
these data could point to a role of oxygen radicals in colon carcinogenesis induced by a chronic infection with s. bovis .
the mechanism could be similar to the one suspected for the development of gastric carcinomas after persisting h. pylori infections since a cecropin - like h. pylori peptide , hp ( 220 ) , was found to be a monocyte chemoattractant and activated the monocyte nadph oxidase to produce oxygen radicals .
presently it would still be important to know whether the increased presence of s. bovis in colonic cancers and polyps results from the preferential bacterial colonization of these cancers and their precursors or whether s. bovis represents a carcinogen that is causally involved in gastrointestinal cancer .
however , our study presents a significant association of streptococcus bovis with malignant gastrointestinal diseases .
the significant association of s. bovis with malignant gastrointestinal diseases compared to its association with non - malignant gastrointestinal diseases presented in this study confirms the previous studies about the association between this bacterium and colorectal cancer and may support the idea that there is correlation between this bacterium and the malignant gastrointestinal diseases .
 
 streptococcus bovis is a gram - positive bacterium causing serious human infections , including endocarditis and bacteremia , and is usually associated with underlying disease . 
 the aims of the current study were to compare prevalence of the bacterium associated with malignant and nonmalignant gastrointestinal diseases and to determine the susceptibility of the isolated strains to different antimicrobial agents . 
 the result showed that the prevalence of s. bovis in stool specimens from patients with malignant or with nonmalignant gastrointestinal diseases was statistically significant . 
 this result may support the idea that there is correlation between s. bovis and the malignant gastrointestinal diseases . 
1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions