the cyclic amp ( camp ) pathway plays fundamental roles in the nervous system , where it is prominently involved in synaptic plasticity and memory formation ( kahsai and zars , 2011 , kandel et  al . , 2014 ) .
previous studies in vertebrate and invertebrate models have shown that camp can propagate from dendrites to the cell body of neurons ( bacskai et  al . , 1993 , hempel et  al . , 1996 ) , in line with the properties of a small diffusible molecule .
however , a local mode of action for camp has also been proposed , whereby camp signals are localized to the periphery of neurons  namely , dendrites  creating a camp microdomain ( castro et  al . , 2010 , li et  al . , 2015 , neves et  al . , 2008 , nicol et  al . , 2011 , nicoll and schmitz , 2005 , tomchik and davis , 2009 ) . while the existence of camp microdomains in neuronal dendrites is disputed based on the experimental and theoretical data ( calebiro and maiellaro , 2014 , gervasi et  al . , 2010 ,
2014 ) , very little is known about possible camp compartmentation in axons and how this may exert local effects at the presynaptic site . in particular , it is unclear how biochemical signals may spread from presynaptic boutons through the axon . to investigate this question in  vivo
, we used the neuromuscular junction ( nmj ) of drosophila melanogaster , which displays different forms of synaptic plasticity ( collins and diantonio , 2007 , frank , 2013 , koon et  al . , 2011 ,
2013 ) , many of which are dependent on camp signaling ( griffith and budnik , 2006 , olsen and keshishian , 2012 ) .
both structural and functional properties of larval neuromuscular synapses are heterogeneous , varying between boutons belonging to the same motor neuron ( paul et  al . , 2015 ,
how such site - specific synaptic differentiation may be achieved at high spatial resolution is currently unknown , though it is tempting to speculate that local camp signals play a role .
thus , the developing drosophila nmj is a powerful model to investigate the role of camp in synaptic plasticity under physiological conditions . in this study
, we focused on glutamatergic type ib motor neurons ( hoang and chiba , 2001 ) , which are structurally regulated via g - protein - coupled receptors ( gpcrs ) for octopamine ( koon et  al .
stimulation of these receptors has been shown to induce synaptic bouton outgrowth via a camp- and creb - dependent pathway ( koon et  al . , 2011 ) .
therefore , we set out to measure spatiotemporal patterns of octopamine - induced camp signals in these neurons .
to this end , we transgenically expressed a fret ( frster resonance energy transfer)-based sensor for camp ( epac1-camps ; nikolaev et  al . ,
2004 ) , which has previously been used to image camp levels in central drosophila neurons ( shafer et  al . , 2008 ) .
our results reveal that camp signals are confined to their initiation site , the individual synaptic bouton , and suggest a highly efficient  local mechanism for controlling site - specific synaptic plasticity .
to investigate camp changes in  vivo and in real time upon octopamine stimulation , we expressed epac1-camps ( nikolaev et  al . , 2004 ) in third instar drosophila larvae using a motor - neuron - specific promoter ( figures 1a and 1h ) .
the whole larvae were perfused with increasing concentrations of octopamine applied to the bath with a pipette . for normalization at the end of each experiment ,
the preparations were stimulated with the broad phosphodiesterase ( pde ) inhibitor ibmx , followed by the direct adenylyl cyclase ( ac ) activator forskolin to maximally activate the epac1-camps sensor ( figures 1b and 1i ) .
in particular , a 10 m concentration of octopamine produced a clear response in the boutons but only a minor effect in the distal axon , while stimulation with higher concentrations ( 10 m ) elicited robust camp responses in both compartments ( figures 1b and 1c ) . the potency of octopamine in eliciting camp fret signals showed a 3-fold higher sensitivity in the boutons compared to the distal axon ( figure  1c ) .
next , we aimed to understand whether camp fret signals generated in the periphery of the motor neuron could reach the cell body . to this end
, we analyzed the extent of the camp fret signals , in space and time , evoked by 10 m octopamine , a concentration that induces structural neuronal plasticity ( koon et  al . , 2011 ) .
we found that , upon generalized application of octopamine , the amplitude of the camp response was similar in the different synaptic boutons analyzed ( figure  1d , blue region ) .
however , in the axon , the amplitude of the camp response decreased nearly exponentially with distance , reaching virtually undetectable levels at 50  m from the most proximal bouton ( figure  1d , green region ) .
similar to the signal amplitude , the speed of the camp response was higher in the boutons and in the distal portion of the axon than in more proximal regions ( figures 1e and 1f ) .
to better illustrate the propagation of camp , instead of showing the direct fret change at different time points after stimulation , we calculated the first time derivative of the fret data . with this alternative method
, the color and intensity of each pixel indicate the rate at which camp levels were increasing ( red ) or decreasing ( blue ) at a given time point ( figure  1 g ; movie s1 ) .
the analysis clearly showed that the production of camp began in the boutons and only later reached a peak in the nearby portion of the axon ( figure  1 g ; movie s1 ) .
finally , we monitored the octopamine - induced camp fret signals directly in the cell body localized at the level of the ventral nerve cord .
stimulation of transgenic larval preparations with a concentration of 10 m octopamine produced no detectable camp increases in the cell  body , unless pdes were inhibited with ibmx ( figure  1i ) .
moreover , the concentration - response curve showed that the cell body was 100-fold less sensitive toward octopamine than  the boutons ( figure  1j ) .
control experiments excluded the  possibility that the intracellular biosensor concentration or differences in the saturation of the sensor at rest might affect the amplitude and the kinetics of the camp response in the different intracellular compartments ( figures s1a  s1d ) . in order to determine whether camp fret signals in the cell body induced by high concentrations of octopamine
might be caused by camp generated in the bouton , we repeated the camp measurements in neurons with a severed axon .
the resulting concentration - response curve of octopamine was indistinguishable from that obtained in intact neurons , suggesting that the camp in the cell body did not originate from the periphery ( figure  1j , dashed line ) .
taken together , our data reveal the formation of a steep camp  gradient upon generalized stimulation with octopamine , where  camp fret signals triggered in the boutons propagated only into the first portion of the axon but did not reach the cell body . to investigate the degree of camp signal compartmentalization , we performed a series of experiments in which octopamine was applied locally by means of iontophoresis ( figure  2a ) .
the delivery of octopamine with increasing ejection currents was well within the linear range of the iontophoresis system ( figure  s2a ) . for our experiments we chose a short stimulation ( 1 s ) to minimize the extracellular diffusion of octopamine ( figure  s2b ) .
such stimuli produced full activation of the receptors located on a single bouton but no equilibrium in camp levels ( figures 2b and 2c ) .
stimulation of the most distal bouton with a saturating octopamine pulse ( 100 na , 1 s ) resulted in a rapid and confined increase of camp within the targeted bouton ( figures 2c2e ) , which reverted to baseline within approximately 20  s ( figures 2c and 2e ) .
the very high sensitivity of the sensor allowed us to fully detect the physiologically relevant range in camp ( figures s2c  s2e ) , which can lead to activation of downstream targets .
remarkably , the response to such localized stimuli was confined to the stimulated bouton and spread no further than the adjacent one ( figures 2c2e ) , where a minor response was seen which could be attributed to the diffusion of octopamine ( figure  s2b ) .
overall , the camp gradient extended to no more than 15  m from the stimulated bouton ( figure  2d ) .
the time - derivative analysis of the fret data reveals the very local increase of camp ( indicated in red ) in the stimulated bouton ( figure  2e ; movie s2 ) , which was followed by a slower return to basal levels ( indicated in blue in figure  2e ) .
taken together , our results provide direct evidence that camp can be confined to a single synaptic bouton of a neuronal axon and that , in these circumstances , camp diffusion in  vivo must be significantly hindered .
the data presented earlier show that the boutons , axon , and cell body are independent camp signaling compartments that can , at least under certain circumstances , signal differentially .
it has been suggested that cell morphology and local differences in the surface - to - volume ratio lead to the formation of camp gradients between different parts of a cell , with higher camp concentrations in finer structures ( neves et  al . ,
, we found larger camp fret signals in the boutons than in the cell body ( figure  1j ) .
however , we observed a stronger camp response in boutons , which have a larger diameter than the axon ( 3- versus 1 m  , respectively ; figures 1b and 1d ) .
furthermore , we monitored a greater camp response in big ( > 2 m  ) than in small ( < 2 m  ) boutons ( figure  3a ) .
this indicates that , in our system , camp fret signals are potentiated in larger boutons .
we then evaluated the contribution of the proteins responsible for the formation and degradation of camp signals , i.e. , receptors , acs , and pdes ( calebiro and maiellaro , 2014 ) .
first , we assessed the contribution of octopamine receptors to the camp gradient induced by generalized octopamine stimulation .
to do so , we monitored camp changes in bouton , axon , and cell body to increasing concentrations of forskolin ; hence , downstream of octopamine receptors .
bypassing receptor activation led to the formation of a gradient between bouton and cell body ( figure  3b ) that was similar to the one seen by octopamine activation , suggesting that mechanisms other than the localization of the receptors are responsible for the differences observed between these two compartments .
however , forskolin induced similar changes in bouton and axon ( figure  3b ) , implying that receptor localization plays a role in the octopamine - induced camp gradient formation between these regions . to further corroborate this hypothesis , we compared the camp response induced by iontophoretic delivery of octopamine to the bouton or to the axon .
the axon showed virtually no local camp response ( figure  3c ) ; therefore , we can infer the absence of functional octopamine receptors in this compartment .
next , we analyzed the contribution of pdes to the octopamine - induced camp gradient .
although it is undisputed that pdes play a critical role in the dynamics of the camp signal , their contribution to diffusion and generation of camp gradients or compartments remains less clear ( conti et  al . , 2014 ) .
when pde activity was blocked with ibmx in the presence of octopamine , the camp fret signals were uniformly elevated along the motor neurons ( figure  3d ; for comparison , see figures 1e and 1i ) .
the camp signal in the cell body was of the same magnitude as in the periphery , regardless of the applied octopamine concentration ( figure  s3 ) .
furthermore , we compared the initial rate of octopamine - induced camp accumulation in the presence of ibmx in the bouton versus that in the cell body as a readout of ac activity .
no significant differences were observed in the rate of camp accumulation in the cell body and bouton , indicating that octopamine - induced ac activity was similar in the analyzed compartments ( figure  3e ) .
additionally , we analyzed the expression pattern of the pdes . to achieve this , we took advantage of a newly developed drosophila line in which the endogenous pde dunce is tagged with egfp ( dnc - egfp - dnc ; nagarkar - jaiswal et  al . ,
staining against horseradish peroxidase ( hrp ) was used to visualize presynaptic arborizations , and anti - gfp staining showed the expression of the dnc - egfp - dnc protein ( figure  3f ) .
we found that pdes appeared largely concentrated at the plasma membrane of the synaptic boutons .
interestingly , pdes were also localized between two neighboring boutons , reminiscent of a  fence ,  which could obstruct the diffusion of camp ( figure  3f , yellow arrows ) .
the dnc - egfp - dnc protein was undetectable in the axons ( figure  3f , white arrow ) , while it showed a strong signal in the cell bodies ( figure  s4a ) .
together , these data indicate that high pde activity contributes to a low camp concentration in the cell body and that the pde distribution at the nmj hinders camp diffusion from one bouton to the next . at the drosophila nmj , glutamate
release from motor neurons is regulated at the level of individual active zones , the specialized presynaptic sites of vesicle exocytosis ( ehmann et  al . , 2014 ,
, 2013 , peled and isacoff , 2011 , schmid et  al . , 2008 ) .
several studies have described that active - zone properties are not homogeneously distributed across motor neurons .
average ultrastructural and functional active - zone features differ between boutons ( ehmann et  al . , 2014 , paul et  al . , 2015 , peled and isacoff , 2011 ) and functionally similar active zones can cluster within boutons ( melom et  al . , 2013 )
the confinement of camp fret signals to a single synaptic bouton might explain how structural and functional diversity is achieved among different boutons . to test this hypothesis , we evaluated whether disturbing the camp metabolism could , consequently , perturb the structural gradient observed along the type ib motor neuron on muscle 6/7 .
this gradient is manifested as an increase in size and active - zone number of terminal boutons compared to more proximal boutons ( paul et  al . ,
we compared wild - type ( wt ) and pde mutant flies ( dunce ) ( davis and kiger , 1981 ) .
it hydrolyzes camp at a significantly slower rate than the wt and should , therefore , be less efficient in segregating camp signals .
mutant animals show impaired learning , memory deficits , and altered motor neuron terminal growth ( zhong et  al . , 1992 , zhong and wu , 2004 ) .
however , whether structural differentiation of synaptic boutons is preserved in the mutants has , to our knowledge , so far not been addressed . indeed , in dunce mutants , the wt gradient in active - zone numbers per bouton was diminished , and the difference in bouton area was reduced ( figures 4a and 4b ) .
these data demonstrate that less efficient degradation of camp , which will lead to a spreading out of local camp increases , perturbs the structural heterogeneity of synaptic boutons .
thus , these results are consistent with a site - specific control of synaptic plasticity by local camp .
in this study , we genetically expressed the camp sensor , epac1-camps , at the drosophila nmj to monitor and quantify spatiotemporal camp dynamics induced by octopamine stimulation .
our results reveal an unexpectedly high degree of camp compartmentalization in motor neurons , which may serve as a basis for local synaptic plasticity , with camp fret signals being ultimately limited to single synaptic boutons .
we identify three camp signaling compartments within the motor neuron : boutons , axon , and cell body . for each cellular compartment
specifically , we find that boutons constitute the most reactive compartment of the motor neuron in terms of camp accumulation .
our results demonstrate that the production of camp is heterogeneous among boutons , with stronger responses to octopamine in large boutons than in smaller ones , possibly related to the increased synaptic strength measured at large boutons ( paul et  al . , 2015 ) .
moreover , the activity and the specific localization of pdes within the synaptic bouton prevent the propagation of camp to the cell body and its diffusion from one bouton to the next .
in contrast to the boutons , octopamine receptors seem to be absent or inaccessible in the axon , and pdes were not detected . accordingly , camp fret signals recorded in the axon differ from those in boutons in terms of amplitude and kinetics .
hence , the axon emerges as the second independent , but not isolated , camp signaling compartment .
interestingly , and in contrast to dendrites ( bacskai et  al . , 1993 , hempel et  al . , 1996 ,
it remains to be clarified whether this may relate to functional differences between axons and dendrites , between species , or between specific neuron types .
finally , we determined the cell body as the third camp signaling compartment within the motor neuron .
we show that the cell body has a very low sensitivity toward octopamine and demonstrate that camp fret signals generated in the cell body are not affected by its physical isolation from the axon .
high pde activity in the cell body contributes to this local suppression of camp signaling and may prevent spillover activation of camp effectors in the cell body and in the nucleus .
our evidence of camp microdomains restricted to single boutons provides a biophysical basis for the local control of synaptic plasticity .
we show that spatially constrained camp changes help to establish differences in morphology and synaptic content of boutons , suggesting that local camp , bouton structure , and synapse formation are intimately linked ( ehmann et  al . , 2014 ,
the observed confinement of camp supports the notion that individual synaptic boutons may represent largely autonomous signaling units , which can receive and integrate signals independently of the other .
the concept that camp could act as a local messenger was postulated almost 40 years ago ( corbin et  al . , 1977 ) .
the existence of camp microdomains has been demonstrated in other cell types ( e.g. , cardiomyocytes ) ( zaccolo , 2011 ) .
however , in neurons , there are contradicting experimental lines of evidence and simulations concerning the existence of camp microdomains ( calebiro and maiellaro , 2014 ) .
our data clearly show that camp can act as a local messenger upon physiological stimulation of a neuron .
the detailed spatiotemporal analysis of the dynamics of camp reveals that this messenger can be restricted at the micrometer level to induce highly localized physiological responses .
a dna fragment containing the whole epac1-camps coding sequence flanked by an agei site at the 5 end and a kpni site at the 3 end was generated by pcr amplification using epac1-camps in pcdna3 ( invitrogen ) as a template and the following primers:5 acataccggtgacactatagaatagggcc ; 3 aagagggtaccaatcgaaattaatacgactcac .
the amplified dna fragment was then cloned downstream of five copies of the upstream activation sequence ( uas ) in the pjfrc7-derived ptl412 vector , by inserting it between the kpni and agei sites .
the obtained plasmid was sent to bestgene to generate the 20xuas - epac1-camps flies by targeted germline transformation .
flies were reared on cornmeal / agar media supplemented with yeast and kept at 25c . to obtain selective expression of epac1-camps in motor neurons , we crossed flies expressing gal4 under the control of the ok6 promoter ( sanyal , 2009 ) .
the dnc - egfp - dnc ( ywmi{pt - gfstf.2}dnc ) line was obtained from the bloomington drosophila stock center .
dunce and canton - s stocks ( figure 4 ) were provided by andreas thum . in order to expose the nmj ,
third instar larvae were dissected in ice - cold calcium - free hl-3 solution ( stewart et  al . , 1994 ) .
the larvae were immobilized , dorsal side up , on a sylgard 184 ( sigma - aldrich ) pad with two sharp pins .
a cut was made along the dorsal midline , and after pinning down the body wall , the internal organs were carefully removed while severing the tracheal connections to the muscles .
larvae were placed in a recording chamber and imaged at room temperature in 2  ml hl-3 solution supplemented with 10  mm l - glutamate to desensitize postsynaptic receptors and thus prevent movement of the larvae .
agonists were applied in the bath with a pipette . unless otherwise noted , the experiments were performed on type ib motor neurons innervating muscle 13 in hemisegments a2 and a3 of male third instar larvae .
the time constant (  ) of camp accumulation was calculated using a non - linear regression ( monoexponential phase decay ) .
ratiometric fret imaging was performed using an upright epifluorescence microscope ( axio observer , zeiss ) equipped with a water - immersion objective ( 63/1.1 numerical aperture ) ; a xenon lamp coupled to a monochromator ( visiview , visichrome ) ; filters for cfp ( 436/20 , 455lp dichroic ) and yellow fluorescent protein ( yfp ; 500/20 , 515lp dichroic ) excitation ; a beam splitter ( dualview , photometrics ) with a 505lp dichroic mirror and emission filters for cfp ( 480/30 ) and yfp ( 535/40 ) ; and an electron - multiplied charge - coupled device ( emccd ) camera ( evolve 512 , photometrics ) .
cfp and yfp images upon cfp excitation were captured every 5  s or every 300  ms with 80  ms of illumination time .
fret was monitored in real time with the metafluor 5.0 software ( molecular devices ) as the ratio between yfp and cfp emissions .
the yfp emission was corrected for direct excitation of yfp at 436  nm and the bleedthrough of cfp emission into the yfp channel , as previously described ( brner et  al . , 2011 ) .
local delivery of octopamine and rhodamine was performed using a high - speed iontophoresis system with capacitance compensation ( mvcs-02c , npi electronic ) .
the headstage was mounted on a micromanipulator ( nmn-21 , narishige ) , allowing precise positioning of the glass microelectrode .
high - resistance ( 7090 m ) microelectrodes were filled with 10 m octopamine or rhodamine dissolved in 150  mm na2hpo4 ( ph 7.2 ) , supplemented with 5% bsa . to allow visualization of the microelectrode tip during fret imaging , the tip of the glass microelectrode was coated with alexa fluor 488-labeled bsa as previously described ( sasaki et  al . , 2012 ) . during the experiments ,
larvae were continuously superfused with hl-3 solution supplemented with 10  mm glutamate in the opposite direction of the iontophoretic delivery , using a custom - made perfusion system . for the visualization of bouton structure and active zones
( 1:1,000 ; life technologies , # a11122 ) and mouse monoclonal antibody ( mab ) brp ( 1:250 ; wagh et  al . , 2006 ) .
secondary antibodies used were cy3-conjugated goat -mouse ( invitrogen ) , af488-conjugated goat -rabbit ( 1:500 ; life technologies , # a11008 ) , and cy3- or alexa fluor 488-conjugated goat -horseradish peroxidase ( -hrp ) ( 1:250 ; jackson immunoresearch laboratories antibodies ) .
bruchpilot ( brp ) puncta per bouton were quantified manually using the four terminal boutons of type ib branches .
distal boutons were located at the end of bouton chains , whereas proximal boutons were closer to the entry site of the motor neuron at the nmj .
different genotypes were stained under the same conditions in the same vial and were analyzed by blinded observers .
confocal image stacks were obtained with a line - scanning confocal sp5 system ( leica ) equipped with a 1.2 numerical aperture 63 water - immersion objective . statistical analyses and curve fitting
differences between means were assessed by a two - tailed student s t test ( for two groups ) and one- or two - way anova , followed by a bonferroni s post hoc test ( for three or more groups ) .
 summarythe second messenger cyclic amp ( camp ) plays an important role in synaptic plasticity . 
 although there is evidence for local control of synaptic transmission and plasticity , it is less clear whether a similar spatial confinement of camp signaling exists . here 
 , we suggest a possible biophysical basis for the site - specific regulation of synaptic plasticity by camp , a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner . by exploiting the octopaminergic system of drosophila , which mediates structural synaptic plasticity via a camp - dependent pathway 
 , we demonstrate the existence of local camp signaling compartments of micrometer dimensions within single motor neurons . 
 in addition , we provide evidence that heterogeneous octopamine receptor localization , coupled with local differences in phosphodiesterase activity , underlies the observed differences in camp signaling in the axon , cell body , and boutons . 
Introduction
Results
Discussion
Experimental Procedures
Author Contributions