90408028 to ct zhang and 10747150 to f gao ) . funding for open access charge
 the genome of sorangium cellulosum has recently been completely sequenced , and it is the largest bacterial genome sequenced so far . in their report , schneiker et al . 
 ( in complete genome sequence of the myxobacterium sorangium cellulosum , nat . 
 biotechnol . , 
 2007 , 25 , 12811289 ) concluded that  in the absence of the gc - skew inversion typically seen at the replication origin of bacterial chromosomes , it was not possible to discern the location of oric. in addition , the complete genome of microcystis aeruginosa nies-843 has also been recently sequenced , and in this report , kaneko et al . 
 ( in complete genomic structure of the bloom - forming toxic cyanobacterium microcystis aeruginosa nies-843 , dna res . , 
 2007 , 14 , 247256 ) concluded that  there was no characteristic pattern , according to gc skew analysis. therefore , oric locations of the above genomes remain unsolved . using ori - finder , a recently developed computer program , in both genomes , we have identified candidate oric regions that have almost all sequence hallmarks of bacterial orics , such as asymmetrical nucleotide distributions , being adjacent to the dnan gene , and containing dnaa boxes and repeat elements . 
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