precise evaluation of the developmental stage forms an integral part of both diagnosis and treatment plan for pediatric patients .
different authors have advocated various methods of determining skeletal maturity based on radiographs of specific structures , such as epiphysis - diaphysis fusion of long bones , medial extremity of the clavicle , epiphyseal head of the first rib , epiphyseal union of the anterior iliac crest , and fusion of the sphenoid bone with the basilar part of the occipital bone . however , these skeletal methods not only present some inconveniences in view of the variability of bone maturation , as influenced by environmental factors , but necessitate additional radiographs as well .
the hand - wrist radiograph has been one of the most commonly used methods for skeletal developmental assessment .
panoramic and lateral cephalometric radiographs form a part of essential diagnostic examination for the comprehensive diagnosis and treatment planning of an orthodontic patient .
apart from its use in studying skeletal , dental , and soft - tissue structures , the usefulness of lateral cephalometric radiographs for assessing maturation has also been studied .
a distinct advantage of cervical maturity evaluation is that it does not entail extra radiation exposure for the orthodontic patient .
hand - wrist and cervical maturation methods are highly correlated , therefore it does not justify to take an extra hand wrist radiograph for skeletal maturation determination .
hassel and farman suggested six stages of classification of the cervical vertebrae based on the shape of the second to the fourth vertebrae on lateral cephalogram .
apart from viewing maxillomandibular structures , an orthopantomogram can also be used to assess the dental maturity of the patient .
dental development can be assessed from either the phase of tooth eruption or the stage of tooth calcification , with the latter being more reliable .
proposed a method for estimation of the subject 's dental maturity based on eight calcification stages , which span from the first sign of tooth calcification to apex closure for the seven left permanent mandibular teeth .
however , short time intervals between different developmental stages may adversely affect the possibility of identifying the relationship between tooth development and maturation .
the third molar offers a unique advantage over other teeth because its development tends to continue for a longer period and until a later age ( 8 - 18 years ) .
the continuation of third molar development during adolescence provides a different point of reference from the other teeth . if a positive correlation between third molar development and general growth can be evaluated , it would be possible to use the third molar as a growth indicator in pubertal patients .
this study has been conducted to estimate dental maturity using the demirjian index ( di ) for the mandibular third molar , to investigate the relationships between dental maturity and cervical vertebral skeletal maturity among growing patients , and to evaluate the clinical value of the third molar as a growth evaluation index .
the aims and objectives of this study were : 
 to estimate dental maturity using the di for the mandibular third molarto investigate the relationship between dental maturity and skeletal maturity among growing patientsto evaluate the use of the mandibular third molar as an adjunctive tool for assessment of adolescent growth in combination with cervical vertebraeto evaluate the clinical value of the third molar as a growth evaluation index . 
  to estimate dental maturity using the di for the mandibular third molar to investigate the relationship between dental maturity and skeletal maturity among growing patients to evaluate the use of the mandibular third molar as an adjunctive tool for assessment of adolescent growth in combination with cervical vertebrae to evaluate the clinical value of the third molar as a growth evaluation index .
the samples were derived from panoramic , lateral cephalometric radiographs of 300 male and 315 female subjects registered as patients at the department of orthodontics at government dental college and hospital , ahmedabad , gujarat , india .
the age range of the sample in females was 9 - 18 years with the mean age of 14 years , and in males was 10 - 18 years with the mean age of 13.4 years .
the following criteria in individuals were used for the selection of subjects : 
 well - nourished and free of any known serious diseasenormal growth and dental development ; no missing teeth or supernumerary teethno congenital oral or maxillofacial anomalies such as cleft lip and palate . 
 
well - nourished and free of any known serious disease normal growth and dental development ; no missing teeth or supernumerary teeth no congenital oral or maxillofacial anomalies such as cleft lip and palate . in this study ,
the lower left third molar has been used as a sample because of higher estimation errors in calculating the maturation of the upper molar than the lower molar .
the root of the upper third molar may overlap with anatomic structures such as the palate , the inferior border of the zygomatic arch , or the maxillary sinus septum .
cases in which the development of the left and right third molars differed remarkably or in which developmental anomalies were observed were excluded .
in which one of eight stages of calcification ( a to h ) was assigned to the third molar tooth [ table 1 and figure 1 ] .
dental calcification stages using di dental calcification stages using the di the cervical vertebrae maturation indicators ( cvmi ) were evaluated by dividing the second , third , and fourth vertebrae into six groups depending on their maturation patterns on lateral cephalogram using the classification of hassel and farman [ table 2 and figure 2 ] .
each sample is assigned di ( a to h ) based on the mandibular third molar development and cervical maturation stage based on skeletal maturation .
all the data thus compiled from the lateral cephalogram and panoramic radiographs of the samples were subjected to appropriate statistical analysis .
we performed statistical analysis using microsoft office excel 2007 ( microsoft ) and ibm - spss version 22 software ( ibm inc . ) .
the mean values of chronologic age in males and females were calculated at each stage of the di .
the median values of cvmi stages were calculated at each stage of the di in males and females . to study the relationship between di and the cvmi , the frequency and the percentage distribution of the stages of calcification were recorded for each tooth , and
the pearson chi - square test ( x ) value and correlation coefficient ( r ) were estimated to determine the relationships between di and cvmi .
independent sample t - tests were performed for comparison of mean age at different stages of di between males and females .
the following criteria in individuals were used for the selection of subjects : 
 well - nourished and free of any known serious diseasenormal growth and dental development ; no missing teeth or supernumerary teethno congenital oral or maxillofacial anomalies such as cleft lip and palate . 
 
well - nourished and free of any known serious disease normal growth and dental development ; no missing teeth or supernumerary teeth no congenital oral or maxillofacial anomalies such as cleft lip and palate . in this study ,
the lower left third molar has been used as a sample because of higher estimation errors in calculating the maturation of the upper molar than the lower molar .
the root of the upper third molar may overlap with anatomic structures such as the palate , the inferior border of the zygomatic arch , or the maxillary sinus septum .
cases in which the development of the left and right third molars differed remarkably or in which developmental anomalies were observed were excluded .
in which one of eight stages of calcification ( a to h ) was assigned to the third molar tooth [ table 1 and figure 1 ] .
dental calcification stages using di dental calcification stages using the di the cervical vertebrae maturation indicators ( cvmi ) were evaluated by dividing the second , third , and fourth vertebrae into six groups depending on their maturation patterns on lateral cephalogram using the classification of hassel and farman [ table 2 and figure 2 ] .
each sample is assigned di ( a to h ) based on the mandibular third molar development and cervical maturation stage based on skeletal maturation .
all the data thus compiled from the lateral cephalogram and panoramic radiographs of the samples were subjected to appropriate statistical analysis .
in this study , the lower left third molar has been used as a sample because of higher estimation errors in calculating the maturation of the upper molar than the lower molar .
the root of the upper third molar may overlap with anatomic structures such as the palate , the inferior border of the zygomatic arch , or the maxillary sinus septum .
cases in which the development of the left and right third molars differed remarkably or in which developmental anomalies were observed were excluded .
in which one of eight stages of calcification ( a to h ) was assigned to the third molar tooth [ table 1 and figure 1 ] .
the cervical vertebrae maturation indicators ( cvmi ) were evaluated by dividing the second , third , and fourth vertebrae into six groups depending on their maturation patterns on lateral cephalogram using the classification of hassel and farman [ table 2 and figure 2 ] . each sample is assigned di ( a to h ) based on the mandibular third molar development and cervical maturation stage based on skeletal maturation .
all the data thus compiled from the lateral cephalogram and panoramic radiographs of the samples were subjected to appropriate statistical analysis .
we performed statistical analysis using microsoft office excel 2007 ( microsoft ) and ibm - spss version 22 software ( ibm inc . ) .
the mean values of chronologic age in males and females were calculated at each stage of the di .
the median values of cvmi stages were calculated at each stage of the di in males and females . to study the relationship between di and the cvmi , the frequency and the percentage distribution of the stages of calcification were recorded for each tooth , and
cross - tabular statistics were performed . the pearson chi - square test ( x ) value and correlation coefficient ( r ) were estimated to determine the relationships between di and cvmi .
independent sample t - tests were performed for comparison of mean age at different stages of di between males and females .
tables 3 and 4 show the mean age for each stage of di in males and females , respectively . although the mean ages at different stages of di individually indicate that lower third molar development is completed earlier in males than in females , it was statistically insignificant except in stage d [ table 5 ] . mean chronological age along with the development of the third molar ( di ) ( male )
mean chronological age along with the development of the third molar ( di ) ( female ) comparison of the mean age between males and females at different stages of di in this study , the results of the chi - square test show the statistically significant interdependence of di and cvmi in males ( p < 0.0001 ) [ degree of freedom ( df ) = 35 ] [ table 6 ] as well as in females ( p < 0.0001 ) ( df = 28 ) [ table 7 ] .
interdependence between di and cvmi ( male ) , chi - square test interdependence between di and cvmi ( female ) , chi - square test there was found a statistically significant positive correlation between the development of the third molar ( di ) and cvmi in both males ( r = 0.81 ) ( p < 0.0001 ) and females ( r = 0.72 ) ( p
< 0.0001 ) . di and mean age also have a high positive correlation in both males ( r = 0.79 ) ( p < 0.0001 ) and females ( r = 0.80 ) ( p < 0.0001 ) .
in addition , cvmi and mean age have a high positive correlation in both males ( r = 0.84 ) ( p < 0.0001 ) and females ( r = 0.84 ) ( p
correlation between di , cvmi , and chronological age ( male ) , pearson correlation statistical test correlation between di , cvmi , and chronological age ( female ) in males , distribution of cvmi to di [ table 10 ] shows that di stage b is characterized by their concentration of cvmi stage 2 ( 66.7% ) , di stage c is characterized by their concentration of cvmi stage 3 ( 60.0% ) , and di stage d is characterized by their concentration of cvmi stage 3 ( 40.9% ) and stage 4 ( 50% ) . in females , distribution of cvmi stage to di [ table 11 ] shows that di stage b is characterized by their concentration of cvmi stage 2 ( 42.9% ) and stage 3 ( 42.9% ) , di stage c is characterized by their concentration of cvmi stage 3 ( 53.3% ) and stage 4 ( 33.3% ) , di stage d is characterized by their concentration of cvmi stage 4 ( 31.8% ) and stage 5 ( 43.2% ) , and di stage e is characterized by their concentration of cvmi stage 5 ( 42.9% ) and stage 6 ( 42.9% ) .
frequency distribution of cvmi by di ( male ) frequency distribution of cvmi by di ( female )
maturational indices have been proposed to evaluate skeletal maturity in the growing patient when planning orthodontic / orthopedic treatment or for clinical and research purposes .
the ease of recognizing the stages of dental development and the availability of panoramic radiography are practical reasons for attempting to assess physiologic maturity without resorting to hand - wrist radiograph .
( alara ) principle is especially important for children and young adults , and thus high - radiation methods should not be used frequently to assess growth .
dental maturity , in particular , has an advantage of being easily evaluable during regular dental treatment .
dental maturity is determined by the stage of tooth eruption or the stage of tooth formation .
chertkow indicated that the completion of root formation in the mandibular canines prior to apical closure may be used clinically as a maturity indicator for pubertal growth spurts with the same degree of confidence as the indicators described for hand - wrist radiographs among white children .
however , root formation and apex closure of mandibular canines are completed by the age of 13 years , and most children exhibit active growth up to the age of 16 - 17 years . a study by bolanos et al .
showed that the root formation of the third molar is complete at an average of 18.5 years .
the third molar has a unique feature compared to other teeth in that its development tends to continue over a long period and until a later age .
the continuation of third molar development during adolescence provides a different point of reference from the other teeth .
recent studies have verified that demirjian 's classification system shows the least intraexaminer and interexaminer errors and a high correlation with biological age .
therefore , dental maturity in this study was determined by evaluating the stages of tooth formation of the third molar by demirjian 's method .
the results also show a statistically significant positive correlation between dental maturity ( di ) and skeletal maturity ( cvmi ) consistent with the findings of engstrom et al . , krailassiri et al . , uysal et al . , and kalinowska et al . , who also have suggested a strong relationship between dental maturity and skeletal maturity . for both sexes ,
di stage b showed the highest percentage distribution at stage 2 of cvmi [ tables 10 and 11 ] .
the relationship between skeletal maturity and peak height velocity ( phv ) is well established .
fishman and hagg and taranger found that the appearance of the adductor sesamoid of the thumb indicates the beginning of the pubertal growth spurt ( onset of phv ) , which corresponds to stage 2 of cvmi . in the present study ,
hence , di stage b signifies the prepeak of the pubertal growth spurt or onset of phv in both the sexes . in the present study , for male subjects
, stages c and d corresponded to cvmi stage 3 [ table 10 ] , while for female subjects , stages b and c corresponded to cvmi stage 3 [ table 11 ] .
bjork and helm found that the mp3cap stage heralds the peak of the pubertal growth spurt , which corresponds to fishman 's skeletal maturity indicator 6 ( stage 3 of the cvmi ) .
it can be inferred that di stages c and d in males represent the peak of the pubertal growth spurt , while di stages b and c in females show that the peak of the pubertal growth spurt has not been passed . in this study in males , the samples in demirjian stages e or above are predicted to occur at the stages higher than cvmi stage 3 , and the samples in demirjian stages f are predicted to occur at the stages higher than cvmi stage 4 [ table 10 ] . in females , the samples in demirjian stages e or above
are predicted to occur at stages higher than cvmi stage 4 [ table 11 ] .
in contrast to the study by sun - mi cho et al . where it was shown that in females , the samples in demirjian stages e or above are predicted to occur at the higher stages of cvmi stage 5 .
thus it can be concluded that in females di stage e and in males di stage f correlate that the peak of the pubertal growth spurt has been passed .
the mean ages at different stages of di individually indicate that lower third molar development is complete earlier in males than in females , but it is statistically insignificant except for stage d , which signifies the peak of the pubertal growth spurt in males .
this finding is consistent with the results of bolanos et al . , which showed for males and females similar age distribution , mean age , and developmental stages , with negligible differences in the mean degree of formation of the four molars .
however , findings by mincer et al . , solari et al . , sisman et al . , and golovcencu et al
. showed that lower third molar development is complete earlier in males than in females .
the revealed correlation between lower third molar development and skeletal maturity in this study will allow clinicians to use the mandibular third molar as an adjunctive tool to assess adolescent growth , in combination with cervical vertebrae evaluations .
individual variations should be taken into consideration when using the developmental stage of the third molar in growth evaluations , as third molars are known for their many variations , based on previous studies .
this cross - sectional study has limitations in terms of the evaluation of results because the subjects in this study were in the pubertal growth period and mainly concentrated in di stages c , d , and e. increasing the number of subjects in each stage would further increase the accuracy of results obtained . further longitudinal study with a larger sample size
is recommended for establishing specific guidelines to use the di of the mandibular third molar for growth assessment .

 both males and females show statistically significant positive correlation between the development of the third molar ( di ) and cvmidi stage b signifies the prepeak of pubertal growth spurt in both males and femalesin males , di stages c and d represent the peak of the pubertal growth spurtin females , stages b and c correlate that the peak of the pubertal growth spurt has not been passeddi stage e in females and di stage f in males correlate that the peak of the pubertal growth spurt has been passedboth sexes present similar developmental pace of the third molar , except for di stage d in males that signifies the peak of puberty . 
 
both males and females show statistically significant positive correlation between the development of the third molar ( di ) and cvmi di stage b signifies the prepeak of pubertal growth spurt in both males and females in males , di stages c and d represent the peak of the pubertal growth spurt in females , stages b and c correlate that the peak of the pubertal growth spurt has not been passed di stage e in females and di stage f in males correlate that the peak of the pubertal growth spurt has been passed both sexes present similar developmental pace of the third molar , except for di stage d in males that signifies the peak of puberty .
dental maturity evaluation using the mandibular third molar can be used as an adjunctive tool for adolescent growth assessment in clinical orthodontics .
 objective : this study was done with the following objectives : to estimate dental maturity using the demirjian index ( di ) for the mandibular third molar ; to investigate the relationship between dental maturity and skeletal maturity among growing patients ; to evaluate the use of the mandibular third molar as an adjunctive tool for adolescent growth assessment in combination with the cervical vertebrae ; to evaluate the clinical value of the third molar as a growth evaluation index.materials and methods : samples were derived from panoramic radiographs and lateral cephalograms of 615 subjects ( 300 males and 315 females ) of ages ranging 9 - 18 years , and estimates of dental maturity ( di ) and skeletal maturity [ cervical vertebrae maturation indicators ( cvmi ) ] were made.results:a highly significant association ( r = 0.81 for males and r = 0.72 for females ) was found between di and cvmi . 
 di stage b corresponded to stage 2 of cvmi ( prepeak of pubertal growth spurt ) in both sexes . in males , di stages c and d represent the peak of the pubertal growth spurt . in females , 
 stages b and c show that the peak of the pubertal growth spurt has not been passed . 
 di stage e in females and di stage f in males correlate that the peak of the pubertal growth spurt has been passed.conclusion:a highly significant association exists between di and cvmi . 
 mandibular third molar di stages are reliable adjunctive indicators of skeletal maturity . 
Introduction
Aims and Objectives
Materials and Methods
Selection criteria
Dental maturity evaluation on panoramic radiograph
Cervical vertebrae maturity evaluation on lateral cephalogram
Statistical analysis
Results
Discussion
Conclusion
Financial support and sponsorship
Conflicts of interest