they are characterized by the presence of the following 
 ( 1 ) 
 hydrazone schiff bases of acyl , aroyl , and heteroaroyl compounds have an addition donor sites like c = o .
this versatility has made hydrazones good polydentate chelating agents that can form a variety of complexes with various transition and inner transition metals and have attracted the attention of many researchers .  
a wide varieties of hydrazones and their metal complexes have been studied because of their important properties which can be used in different applications , such as , extraction of some metal ions , microdetermination of metal ions , determination of titanium in bauxite , portland cement , amphibolites granites , and different biological activities , such as antimicrobial [ 2 , 47 ] , antifungal [ 8 , 9 ] , antitumor [ 10 , 11 ] , and insecticides . for these applications
, we are extending this field of compounds for synthesising novel macrocyclic hydrazones .   five dissymmetric tridentate schiff base ligands containing a mixed donor set of onn and ono
were prepared by the reaction of benzohydrazide with the appropriate salicylaldehyde and pyridine-2-carbaldehyde and characterized by ft - ir , h nmr , and c nmr .  
inoue et al .   reported the synthesis and spectroscopic characterization of complexes of ni , cu , zn , and cd with hydrazones schiff bases derived from 6-amino-5-formyl-1,3-dimethyluracil , nicotinic and isonicotinic acids hydrazides .
reported the synthesis and characterization of a macrocyclic hydrazone schiff bases prepared by condensation of 1,4-dicarbonyl phenyl dihydrazide with 2,6-diformyl-4-methyl phenol .
reported [ 1618 ] formation of binuclear complexes for the ligands derived from 4,6-diacetylresorcinol , where the ligands were prepared by condensation of 4,6-diacetylresorcinol ( dar ) with oxalyldihydrazine ( odh ) in the molar ratios ( 1  :  1 ) and ( 1  :  2 ) to afford the corresponding hydrazone , h6la and h4 lb , ligands , respectively .
otomo and nakayama et al .   reported formation of three terdentate hydrazones , all containing the 1-phthalazino grouping in the hydrazine moiety but differing in the heterocyclic substituent in the aldehyde moiety , where the ligands were used as analytical reagents for palladium(ii ) , the optimal conditions for the extractive spectrophotometric determination of palladium(ii ) in the presence of chloride ions being deduced .
these compounds were highly selective and sensitive reagents for palladium(ii ) , since they were not extracted into chloroform from sulfuric acid solutions and did not react with other platinum group metals .
odashima and ishii   reported the synthesis and spectroscopic characterization of four new hydrazones , 2-pyridinecarbaldehyde 3-nitro-2-pyridylhydrazone , 2-pyridinecarbaldehyde 3,5-dinitro-2-pyridylhydrazone ( pa-3,5-nph ) , 2-quinolinecarbaldehyde 5-nitro-2-pyridylhydrazone , and 6-phenanthridinecarbaldehyde 5-nitro-2-pyridylhydrazone .
also useful information on the molecular design of the hydrazone reagent was obtained . a highly sensitive and practical extraction - spectrophotometric method for the determination of nickel with pa-3,5-nph developed and applied successfully to the determination of nickel in steel samples .   in the context of the above applications ,
we have reported here the synthesis and characterization of novel macrocyclic hydrazone schiff bases and their extraction study of some transition metal cations .
all the chemicals used were of analar grade and procured from sigma - aldrich and fluka .
the solvents were saturated with each other before use in order to prevent volume changes of the phases during extraction .
the c , h , and n were analyzed on a carlo - erba 1106 elemental analyzer .
h and c - nmr spectra of ligands in cdcl3 solution were recorded on a bruker dt-400  mhz spectrometer , and chemical shifts are indicated in ppm relative to tetramethylsilane .
aa 929 unicam spectrometer was used for faas measurements with an air - acetylene flame . a ph meter ( metrohm 691 ph meter )
all extractions experiments were performed by using a mechanical flask agitator in 50  cm stoppered glass flasks .
a succinic acid ( 9.19  mmol ) in absolute dry ethanol ( 60  ml ) containing 2 - 3 drops of concentrated h2so4 was refluxed , continuing the heating for 2030 minutes . when the reflux time is complete ,
place about 5  ml chipped ice in a 50-ml beaker and mix it with 15  ml 10% aqueous na2co3 ( sodium carbonate ) ( the ph should be above 7 . if it is not , add more ( 10% ) na2co3 dropwise until the solution is basic ) .
then dry the ether solution in the centrifuge tube by adding several spatula - tip loads of anhydrous na2so4 .  
adipic acid ( 7.92  mmol ) in absolute dry ethanol ( 60  ml ) containing 2 - 3 drops of concentrated h2so4 was refluxed , then continue the heating for 2030 minutes .
when the reflux time is complete , remove the flask from the heating mantle , allow it to cool briefly .
place about 5  ml chipped ice in a 50-ml beaker and mix it with 15  ml 10% aqueous na2co3 ( sodium carbonate)(the ph should be above 7 . if it is not , add more 10% na2co3 dropwise until the solution is basic ) .
then dry the ether solution in the centrifuge tube by adding several spatula - tip loads of anhydrous na2so4 .
mixture of diethyl ester of succinic acid ( 2.22  g ) and hydrazine hydrate ( 98% 2  cc ) in ethanol ( 80  ml ) was refluxed for 4 - 5  h. the reaction mixture was allowed to cool in room temperature , then the cooled solution was poured onto ice cold water .
a mixture of diethyl ester of adipic acid ( 2.22  g ) and hydrazine hydrate ( 98% 2  cc ) in ethanol ( 80  ml ) was refluxed for 4 - 5  h. the reaction mixture was allowed to cool in room temperature , then the cooled solution was poured onto ice cold water .
the dihydrazide of adipic acid obtained was filtered and recrystallized from ethanol . the hot ethanolic solution ( 20  ml ) , of acetylacetone
( 2  mmol , 0.2  g ) , and a hot ethanolic solution ( 20  ml ) , of dihydrazide of succinic acid ( 2  mmol , 0.292  g ) , were mixed slowly with constant stirring .
this mixture was refluxed at ~75c for 9  h in presence of few drops of concentrated hydrochloric acid ( ph~3 ) .
the reaction mixture was allowed to cool in room temperature , then the cooled solution was poured onto ice cold water . on cooling , white - colored precipitate is separated out , filtered , washed with cold etoh , and dried under vacuum over p2o5 yield 50% .
elemental analysis found % ( found atomic mass 420  amu ) , c 51.14 h 5.64 ; n 26.53 for c18h28n8o4 .
the hot ethanolic solution ( 20  ml ) , of acetylacetone ( 2  mmol , 0.2  g ) , and a hot ethanolic solution(20  ml ) , of dihydrazide of adipic acid ( 2  mmol , 0.348  g ) , were mixed slowly with constant stirring .
this mixture was refluxed at ~75c for 9  h in presence of concentrated hydrochloric acid ( ph~3 ) .
the reaction mixture was allowed to cool in room temperature , then the cooled solution was poured onto ice cold water . on cooling , white - colored precipitate is separated out , filtered , washed with cold etoh , and dried under vacuum .
elemental analysis found % ( found atomic mass 420  amu ) , c 55.69 h 6.66 ; n 22.62 for c22h36n8o4 .
aqueous solutions containing 1.5  10  mol l metal chloride in appropriate buffer were equilibrated with equal volumes of the chloroform and dichloromethane solutions of the ligand 4  10  m by shaking in a mechanical shaker at 25c .
in most cases , distribution equilibrium was attained in less than 180  min and a shaking time of 120  min .
the ionic strength of the aqueous phase was 0.1  m kcl in all experiments except those in which the effect of ionic strength was studied .
the copper(ii ) and chrome(iii ) concentrations of the aqueous phase were determined by faas and that of the organic phase from the difference by considering the mass balance .
the preparation of two ligands containing nitrogen and oxygen donor atoms are shown in scheme 1 .
the structures of new compounds were characterized by a combination of ir , ms , h nmr , and c nmr spectral data .
ligand(i)the spectrum showed a strong bands at 1715 and 1587  cm in the spectrum of the schiff base assigned to (c = o ) of carbonyl and azomethine (c = n ) vibrations , respectively .
an intense band at 3111  cm is due to the nh - vibrations of the hydrazine group , a broad medium intense band was at 2930  cm due to methylene groups , and the band at 1068  cm is assigned to hydrazinic (n 
n ) of the ligand [ 2125 ] . in the electron impact spectrum ( figure 1 ) of the ligand
, we confirm the probable formula by showing a peak at 420  amu , corresponding to macrocyclic moiety [ ( c18h28n8o4 ) , calculated atomic mass 420 ] .
the series of peaks in the range , that is , 37 , 40 , 41 , 42 , 44 , 55 , 95 , 96 , 97 , 101 , 121 , 132 , 150 , 179 , 201  amu , and so forth , may be assigned to various fragments .
their intensity gives an idea of stability of fragments ( figure 1 ) . in the h nmr spectrum the ligand(i )
exhibits signals at 2.24  ppm due to ch3c(6h ) , 2.54 due to ch2(4h ) , 3.55 due to ch2co(8h ) and at 5.9  ppm due to nh protons ( figure 3(a)).in the c nmr spectrum ( figure 3(b ) ) of ligand(i ) , and indicated new resonances are 13.80 , 14.58(ch3 ) , 23.74 , 34.90(ch2 ) , 110.96 , 143.99(ch2co ) , 151.77(c = n ) , 173.71(co 
the spectrum showed a strong bands at 1715 and 1587  cm in the spectrum of the schiff base assigned to (c = o ) of carbonyl and azomethine (c = n ) vibrations , respectively .
an intense band at 3111  cm is due to the nh - vibrations of the hydrazine group , a broad medium intense band was at 2930  cm due to methylene groups , and the band at 1068  cm is assigned to hydrazinic (n 
n ) of the ligand [ 2125 ] .   in the electron impact spectrum ( figure 1 ) of the ligand
, we confirm the probable formula by showing a peak at 420  amu , corresponding to macrocyclic moiety [ ( c18h28n8o4 ) , calculated atomic mass 420 ] .
the series of peaks in the range , that is , 37 , 40 , 41 , 42 , 44 , 55 , 95 , 96 , 97 , 101 , 121 , 132 , 150 , 179 , 201  amu , and so forth , may be assigned to various fragments .
their intensity gives an idea of stability of fragments ( figure 1 ) .   in the h nmr spectrum the ligand(i )
exhibits signals at 2.24  ppm due to ch3c(6h ) , 2.54 due to ch2(4h ) , 3.55 due to ch2co(8h ) and at 5.9  ppm due to nh protons ( figure 3(a ) ) . in the c nmr spectrum ( figure 3(b ) ) of ligand(i ) , and indicated new resonances are 13.80 , 14.58(ch3 ) , 23.74 , 34.90(ch2 ) , 110.96 , 143.99(ch2co ) , 151.77(c = n ) , 173.71(co  nh ) . 

ligand(ii)the spectrum showed strong bands at 1717 and 1581  cm in the spectrum of the schiff base assigned to (c = o ) of carbonyl and azomethine (c = n ) vibrations , respectively .
a broad medium intense bands were at 2959 and 2873  cm due to methyl groups and band at 1136  cm is assigned to hydrazinic (n 
n ) of the schiff base [ 2125].in the electron impact spectrum ( figure 2 ) of the ligand(ii ) , we confirm the probable formula by showing a peak at 476  amu , corresponding to macrocyclic moiety [ ( c22h36n8o4 ) , calculated atomic mass 476 ] .
the series of peaks in the range , that is , 38 , 39 , 40 , 41 , 42 , 43 , 54 , 55 , 56 , 96 , 97 , 111 , 121 , 138 , 150 , 165 , 179 , 201 , 205 , 251 , 301 , 302  amu , and so forth , may be assigned to various fragments .
their intensity gives an idea of stability of fragments ( figure 2 ) . in the h nmr spectrum , ligand(ii )
exhibits signals at 2.24 due to ch3c(12h ) , 3.17  ppm due to co 
ch2ch2(8h ) , 2.54  ppm due to ch2cn(4h ) , and 5.9  ppm due to nh protons signal ( figure 4(a ) ) .
all these observations support the infrared conclusions.in the c nmr spectrum figure 4(b ) of ligand , indicated new resonances are 13.82 , 14.18 , 14.45 , 28.58 , 29.88 , 30.47 , 60.66(ch2 ) , 111.04 , 144.08(ch2co ) , 152.07(n =
the spectrum showed strong bands at 1717 and 1581  cm in the spectrum of the schiff base assigned to (c = o ) of carbonyl and azomethine (c = n ) vibrations , respectively .
a broad medium intense bands were at 2959 and 2873  cm due to methyl groups and band at 1136  cm is assigned to hydrazinic (n 
n ) of the schiff base [ 2125 ] . in the electron impact spectrum ( figure 2 ) of the ligand(ii ) , we confirm the probable formula by showing a peak at 476  amu , corresponding to macrocyclic moiety [ ( c22h36n8o4 ) , calculated atomic mass 476 ] . the series of peaks in the range , that is , 38 , 39 , 40 , 41 , 42 , 43 , 54 , 55 , 56 , 96 , 97 , 111 , 121 , 138 , 150 , 165 , 179 , 201 , 205 , 251 , 301 , 302  amu , and so forth , may be assigned to various fragments .
their intensity gives an idea of stability of fragments ( figure 2 ) .   in the h nmr spectrum , ligand(ii )
exhibits signals at 2.24 due to ch3c(12h ) , 3.17  ppm due to co 
ch2ch2(8h ) , 2.54  ppm due to ch2cn(4h ) , and 5.9  ppm due to nh protons signal ( figure 4(a ) ) .
all these observations support the infrared conclusions . in the c nmr spectrum figure 4(b ) of ligand , indicated new resonances are 13.82 , 14.18 , 14.45 , 28.58 , 29.88 , 30.47 , 60.66(ch2 ) , 111.04 , 144.08(ch2co ) , 152.07(n =
c ) , and 172.60(co  nh ) . to the best of our knowledge ,
the stability of a transition metal complex with a polydentate chelate ligand in organic phase depends on a range of factors including : number and type of the donor atoms present , the number and size of the chelate rings formed on complexation . in addition , the stability and selectivity of complexations strongly depend on the donor ability , dielectric constant of the solvent , and shape and size of the solvent molecules . in this research ,
liquid - liquid extraction experiments were performed to examine the efficiency and selectivity of hydrazones ( i ) and ( ii ) in transferring s - metal ions ( li , na , and k ) and d - metal ions ( cu and cr ) from aqueous phase into chloroform .
the results show that the alkali metals ions are not extracted by hydrazones ( e < 3.5% ) .  
also the results ( figures 5(a ) and 5(b ) ) show the extractability of cu and cr from the aqueous phase into organic phase by macrocyclic hydrazones ( i ) and ( ii ) .
it is clear that the extractability results of hydrazones are different for both of the organic solvents . when dichloromethane was used as organic solvent , macrocyclic schiff bases extracted cu ion 98% and cr 94% .
it is interesting that the percentage of the extraction of the metal ions with both hydrazones ( i ) and ( ii ) is high .
these results suggest that n donor ( the presence of the lone electron pair on the nitrogen atom in hydrazone molecules provides their basic properties ) increases the percentage of the extraction of the metal ions .
it can be seen from ( figures 5(a ) and 5(b ) ) that the solvent has an important effect upon the cation extractability .
dichloromethane having a high dielectric constants is favored for the extraction of all the metal ions ; there are similar results in the literature . on the other hand
, the better solvation of the complexes by dichloromethane may be a valuable reason for better extraction . from the extraction data shown in the figures ,
it is clear that the ligands which have n8o4 donor sets show that both of the cationcavity size and the type of binding sites in the ring contribute to the ability of cu ions and cr ions binding .
as shown in the figures , the copper and chrome extraction is high within the ph range of 5.26 , 57(cu ) , 7 - 8 , 77.5(cr ) , respectively .
moreover , transition metals were extracted in the order cu > cr , which are in the same order of decreasing ionic radius .  
if only mononuclear species are extracted , under the condition in which chloride does not take part in the distribution equilibrium , the extraction process may be represented by equation 
 ( 2)cu2+(w)+hnl(o)cul(o)+nh+(w ) , 
 where hnl ( i ) represents the extractant reagent and subscripts ( w ) and ( o ) denote the aqueous and organic phases , respectively .
the extraction constant of the species cul is given by 
 ( 3)k  ext  = [ cul]o[h+]wn[cu2+]w[hnl]o . 
 
when cul is the only extractable species and the metal is present in the aqueous phase predominantly as the cation cu , the metal distribution ratio ( d ) and the extraction constant are related by 
 ( 4)logd = logkext+nph+log[h2l]o . 
  the effect of ph on the extraction of cu and cr ions from kcl media of ionic strength ( i = 0.1  m ) has been studied , the logarithm of the d values obtained was plotted against the corresponding ph values ( according to ( 4 ) a plot of logd against ph at constant 4  10  m of [ hnl ] ) .
a straight line with a slope of about 2 was obtained at i = 0.1 of cu and cr , as shown figures 6(a ) and 6(b ) .
the values represent the number of hydrogen ions released during the formation of metal - ligand complex and intercept log[hnl ] + logk 
 ext .
also figure 7 shows the evolution of logd when increasing the concentration of ligands at constant chloride metal concentration with two different organic solvents . as seen from the plots
, there is a linear relationship between logd and log[l]org , and the slope should be equal to the number of ligand molecules per cation in the extracted species .
therefore , ligands form a 1  :  2 ( l : m ) complex with cu for both solvents .
the influence of kcl in the concentration range of 0.11.0  m on the extraction efficiency of cu was studied in solutions containing 1.5  10  m cu and cr with 4  10 m ligand in organic phase .
the extraction efficiency decreases with increase in ionic strength of the aqueous medium . taking into account ( 5 )
, the extraction constant ( k 
 ext 
 ) at zero ionic strength for this reaction can be correlated with the ionic strength(i ) by 
 ( 5)cu2+(w)+h2l(o)cul(o)+2h+(w ) , 
 ( 6)kext0=kexth+ncu2 + , 
 ( 7)kext = kext0cu2+h+n . 
 according to the debye - huckel limiting law given 
 ( 8)log=0.5zi2  i. 
  the activity coefficient (  
  ) decreases with increase in ionic strength . at the constant ph ,
the activity coefficient of cu decreases as the ionic strength increase , hence k 
 ext decreases .
these symmetrical compounds were obtained with yield more than 50% in some cases . two imines ( schiff bases ) were synthesized .
this is confirmed by a precise review of the scientific background concerning this category of compounds .
the results indicate that h2l(i , ii ) in organic phase extracts efficiently cu and cr in aqueous phase containing 0.1  mol l kcl in the ph range of approximately 57 and 7 - 8 , respectively , at 25c and 2  h stirring .
 two new macrocyclic hydrazone schiff bases were synthesized by reaction of succindihydrazide and adipdihydrazide with acetylacetone . 
 hydrazones have been characterized by elemental analyses and ir , mass , 1h  nmr , and 13c  nmr spectral data . 
 hydrazones have been studied by liquid - liquid extraction towards the s - metal ions ( li+ , na+ , and k+ ) and d - metal ions ( cu2 + and cr3 + ) from aqueous phase to organic phase . 
 the effect of chloroform and dichloromethane as organic solvents over the metal chlorides extraction was investigated at 25  0.1c by using flame atomic absorption . 
 we found differences between the two solvents in extraction selectivity . 
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusion