in this study , somatic cell cloned embryos were successfully produced on the basis of 
 a previous report . briefly
, femoral 
 fibroblasts of an 11-year - old holstein dairy cow from koiwai farm were used as donor 
 cells for somatic cell cloned embryos .
two cloned holstein cows , cody 1 and cody 
 2 , were derived from femoral fibroblasts of the donor holstein cow with the somatic 
 cell cloning technique .
birth weights ( bw ) and other after - birth data of the 
 offspring of cody 2 and of cody 2 are shown in the pedigree charts ( fig .
birth weights ( bw ) and other after - birth data of the somatic cell cloned 
 holstein cow ( cody 2 ) and the offspring are shown in the pedigree 
 charts .
*  holstein cow derived from multiple ovulations for cody 2 and 
 embryo transfer . * * holstein cow and calf derived from artificial 
 insemination ( ai ) for cody 2 . ) .
co1 was born after 
 multiple ovulation treatments on cody 2 and embryo transfer into recipient cows , 
 whereas co2 was born through artificial insemination ( ai ) of cody 2 .
growth performance of the two somatic cell cloned 
 heifers did not differ from that of other cattle born within the same month , with no 
 specific apparent clinical abnormalities .  
birth weights ( bw ) and other after - birth data of the somatic cell cloned 
 holstein cow ( cody 2 ) and the offspring are shown in the pedigree 
 charts .
*  holstein cow derived from multiple ovulations for cody 2 and 
 embryo transfer . *
* holstein cow and calf derived from artificial 
 insemination ( ai ) for cody 2 .
the second generation offspring of the somatic cell clone heifers calved 
 successfully , with co1 producing a male japanese black crossbred calf in the first 
 parturition and then producing a female holstein calf via ai in the second 
 parturition .
reproduction and milk production data were collected from the donor cow ( first  sixth 
 lactation ) , cody 2 ( first  third lactation ) , co1 and co2 ( first  second lactation ) . 

milking cows from koiwai farm ( 563 cows with a mean parity of 2.6 ) were used as the 
 control group .
the primipara age of the month and calving intervals ( days ) were 
 collected as reproduction data .
the calving intervals ( days ) of the donor holstein 
 cow ( first  sixth lactation period ) and the control group were 523 days and 427 days , 
 respectively .
the reproduction performance was particularly reduced in the two 
 somatic cell cloned holstein heifers . for milk productivity , data for the lactation curve and transitions of monthly milk 
 compositions during the first lactation ( fig . 

1 ) were collected on the basis of the dairy herd performance test every 
 month .
the milk yield ( kg ) over 305 days and milk composition rates ( fat , protein 
 and snf ) for each lactation period were measured ( table 1 ) .
the mean milk yield and milk composition rates ( fat , protein and snf ) of the 
 donor holstein cow from the first to sixth lactation periods were 11,015 kg and 
 3.5% , 3.2% and 8.7% , respectively , whereas they were 8,128 kg and 3.7% , 3.3% and 
 8.9% , respectively , for the control group .
 abstract this study examined two female offspring of a somatic cell cloned holstein cow 
 that had reproduction problems and milk production performance issues . 
 the two 
 offspring heifers , which showed healthy appearances and normal reproductive 
 characteristics , calved on two separate occasions . 
 the mean milk yields of the 
 heifers in the first lactation period were 9,037 kg and 7,228 kg . 
 the relative 
 mean milk yields of these cows were 111.2% and 88.9% , respectively , when 
 compared with that of the control group . 
 no particular clinical abnormalities 
 were revealed in milk yields and milk composition rate [ e.g. , fat , protein and 
 solids - not - fat ( snf ) ] , and reproductive characteristics of the offspring of the 
 somatic cell cloned holstein cow suggested that the cloned offspring had normal 
 milk production . 
Methods