fetal cholelithiasis is uncommon during pregnancy although its frequency of diagnosis has increased over the last years , probably due to both the improved accuracy and the increasing use of ultrasound examination in clinical practice.15 despite the remarkable number of fetal scans performed annually worldwide , little is known about the causes , natural history , and clinical significance of echogenic foci in the gallbladder during fetal life , unlike in the pediatric or adult patient.68 we report a case of cholelithiasis in the fetal gallbladder identified sonographically in the late third trimester of pregnancy during a routine obstetric scan .
a 34-year - old - woman , gravid 2 , para 1 , was referred at 35 weeks of gestation for a routine obstetric ultrasound examination in our prenatal center .
high risk pregnancy as the result of a preexisting maternal medical condition or one that had appeared later was excluded .
a healthy prepregnancy body mass index ( bmi ) and a correct weight gain during pregnancy were recorded . a previous ultrasound examination performed in another center at 23 weeks demonstrated a live singleton fetus with no apparent structural abnormalities and normal morphological development .
an ultrasound evaluation was achieved before 35 weeks . in the third trimester , a traditional scan and traditional organ system evaluation scan of the fetus was unremarkable except for a large nonshadowing multiple small echogenic foci within the gallbladder in a transverse image through the fetal abdomen ( fig .
was realized , using different 3-d imaging techniques ( rendering , tomographic ultrasound imaging [ tui ] , virtual organ computer - aided analysis [ vocal ] , angio - power doppler ) ( figs .
a female infant weighing 3650 grams was born at 38 weeks of gestation by cesarean section for previous abdominal delivery , with apgar scores of 9 and 10 at 1 and 5 minutes , respectively . at 1 day of age
a satisfactory health status was confirmed by bilirubin and neonatal screening test for metabolic pathologies . at 2 days of life ,
a normal - sized gallbladder with minimal neonatal cholelithiasis was detected . in consideration of the liver chemistry including hematological indices within normal ranges and reassuring ultrasound result , the infant was discharged on the third day of life .
an ultrasound follow - up was performed at 2 months of life , revealing a normal size gallbladder deprived of echogenic stones , biliary tract anomalies , or obstructions . in consideration of the resolution of abnormal findings , further evaluation was not planned .
in a review in 1928 , potter identified several descriptions of neonatal gallstones and cited 2 poorly documented cases of fetal cholelithiasis diagnosed at the time of autopsy9 , though the first prenatal diagnosis by beretsky and lankin occurred in 1983.2 anomalies of the gallbladder , including sludge and gallstones , are extremely uncommon in fetal life , with an incidence of 0.42% , as reported recently in a large cohort of 9235 pregnancies , with a slightly greater male predilection.5,10 in the last decades , maternal conditions and fetal or obstetrical predisposing risk factors have been proposed to have a causative role , but none of them appears conclusive ( table 1 ) . in the condition placental abruption , fanaroff suggested that the placental cross of hemoglobin transformed into bilirubin increases the indirect bilirubin levels , responsible for sludge or gallstones in the fetal gallbladder.11 conversely , brown proposed that an increase in estrogen levels might predispose to the formation of pigment stones stimulating the secretion of cholesterol and reducing the synthesis of biliary acids.5 in some cases , maternal narcotic use , methadone particularly , should modify gastrointestinal activity with an increased transition time and the formation of solid echogenic material.12,13 augmented cellular degradation is known to predispose to calculus formation in postnatal life .
thus , a corresponding situation could be involved during hemolytic anemia and blood group incompatibilities ; both conditions are recognized as predisposing factors in the development of echogenic material in the bile.14 in addition to the risk factors previously described in the literature , kiserud suggested that chromosomal aberrations , cardiac malformations , gastroschisis , influence of prostaglandin , and possibly prenatal leukemoid reaction may cause the presence of material in the fetal gallbladder.14 taking into consideration the largest single series reported by brown and the equally significant results reported by kiserud , characterized by scans performed for indications , it can be concluded that in the centers receiving a variety of referrals , more than half of the cases with echodense contents of the fetal gallbladder have maternal or fetal complications.5,14 sonographically , after 14 weeks gestation , the fetal gallbladder can be recognized as an anechoic , oblong , teardrop - like structure with a thin echogenic wall , located at the inferior surface of the right lobe of the liver . accurate assessment and identification of the fetal gallbladder require prior visualization of the umbilical vein , because they have a similar appearance .
after 20 weeks of gestation , the fetal gallbladder can be visualized in 37.5% to 64.7% of the cases.4 stones and sludge identified in the fetal gallbladder are almost absent in the second trimester , resulting in a specific phenomenon of the third trimester due to abnormal production , composition , and mode of transportation of bile in the biliary tract.2,15 a wide spectrum of findings regarding homogeneity , echogenicity , and degree of acoustic shadowing can be detected.5,16 echogenic material in the gallbladder may be visualized by transverse sections through the fetal abdomen in the form of single , multiple , or diffuse foci.8 frequently , the echogenic material completely fills the gallbladder lumen.6 unlike in the adult patient , this material produces different degrees of acoustic shadowing .
small echogenic foci from 2 to 3 mm in size and sludge may not cause shadowing if they do not lie in the center of , or in the focal range of , the beam , while foci greater than 3 mm may produce acoustic .
schirmer underlined the importance of the nature of the calculi , concluding that none of calcified ( radiopaque ) stones disappeared spontaneously after birth , contrary to the cholesterol , urate , or pigment stones ( nonradiopaque).17 in the literature , cases of fetal gallstone diagnoses complicated by contracted gallbladder with a stone localization have been reported as no clear finding.1,15 in this condition , the calculi could be confused with liver calcification , calcified liver mass , or meconium peritonitis . in an attempt to precisely specify the relationship between the gallbladder , its content , and nearby structures , we speculated whether a 3-d approach might provide more information in diagnostic processing than a traditional 2-d ultrasonography . by an offline processing , 3-d rendering surface and tui showed the relationship between the gallbladder and the cholelithiasis .
the volume of the gallbladder was measured by vocal , and the visualization of the vessels surrounding the gallbladder was demonstrated by angio - power doppler .
overall , in contrast to conventional 2-d ultrasound that only allows imaging of single planes , the 3-d approach offers several image displays that do not exist in 2-d imaging . in obstetrics , in particular
, it offers a precise demonstration of the normal and abnormal anatomy of the fetus to the obstetrician .
digital storage of volumes permits virtual examinations by reloading of volumes and navigating through them in the absence of the patient .
the value of a 3-d scan is indisputable in specific clinical situations when the traditional scan results are poorly defined , not precise , or not clerly interpreted . in literature
, there is evidence suggesting that 3-d ultrasound improves the diagnosis increasing the visualization quality of the anatomical landmarks , with minimal impact on the examination time required .
furthermore , cardiac or brain volumes can be successfully analyzed off - line locally or sent by internet to experts for review.18 to date , after an initial enthusiasm for the innovative techniques in obstetric ultrasound and their application in several conditions , an in - depth revaluation about the possible risks related to use , or better the abuse , has been required.18 3-d modalities should be used only in selected cases requiring an effective investigation and analyzed postprocessing only by skilled operators . generally , fetal cholelithiasis appears as a quite clear finding , detectable easily for anatomical reasons and for advanced gestational age at diagnosis time.18 complications are very rare and , in most series , gallstones disappear spontaneously within the first year of life .
only 1 case of a laparoscopic cholecystectomy in a 16-day - old infant has been reported.19 in the review of suchet et al , 30 cases of fetal cholelithiasis with a long - term follow - up in 21 out of 30 babies were described.12 in only 3 patients , a persistent echogenic material in the gallbladder after 2 weeks of life was observed without any abdominal symptoms.12 stringer et al reported a series of 3 male fetuses examined sonographically in late gestation with evidence of gallbladder cholelithiasis.13 postnatally , all 3 were asymptomatic , but they had variable ultrasound findings . in 2 of these , the abnormalities noted sonographically resolved spontaneously in 6 weeks , while in third , a persistent gallbladder  calculus  until 6 months has been detected.15 in the 2 cases described by suma et al , the fetal gallstones showed complete resolution at the follow - up sonography at 2 and 3 months of life without any treatment.3 munjuluri et al described 2 cases treated with ursodeoxycholic acid ( dosage 57 mg / kg / die ) , reporting the complete resolution of gallstones at 2 and 4 months of life , respectively.16 recently , iroh tam and angelides described the first case of fetal gallstones in 3 siblings ( a male term neonate with gallstones and 2 male twins by dizygotic dichorionic pregnancy , twin a with gallstones and twin b with sludge in the gallbladder).1 both siblings with gallstones were treated with ursodeoxycholic acid after birth with complete resolution . in both cases ,
the therapeutic decision was based on undocumented side effects of the infant health and for obtaining a more rapid resolution of the stones.16 in consideration of previous studies and in our experience , fetal cholelithiasis is confirmed to be a self - limiting disease without complications and with a spontaneous resolution in the first year of life . although no morbidity that would be amenable to treatment has been documented , there are still theoretical neonatal risks related to the cholelithiasis therapy , including the possibility of recurrance by the end of treatment with a rare long - term freedom from stones.20 therefore , if further investigations are necessary in order to clarify the potential risks of the pharmacological approach , we discourage some forms of therapy such as ursodeoxycholic acid administration due to unknown and potential side effects in childhood or adulthood . in conclusion , several questions remain unanswered about the etiology , the risks of further gallstone formation , and long - term effects of pharmacological treatment in cholelithiasis detected during pregnancy .
a close follow - up is indicated in these patients until spontaneous resolution is demonstrated by traditional ultrasound .
the 3-d imaging techniques might show some advantages in difficult clinical conditions , through an off - line processing of stored images . considering the high rate of spontaneous resolution ,
the best practice is to reassure parents and to observe the clinical evolution with periodic ultrasound examinations without any treatment .
only future follow - up studies will be able to respond fully to current open questions .
 fetal gallstones and cholelithiasis , detected by routine third trimester ultrasound , have been described in the literature with controversial clinical significance . 
 we report a case of fetal cholelithiasis detected at 35 weeks gestation during a routine scan . 
 the diagnosis was performed using an integrated 2-dimensional ( 2-d ) and 3-dimensional ( 3-d ) ultrasound approach in order to obtain a better definition of the fetal gallbladder and its content . 
 a neonatal follow - up was achieved . 
 the present study has a twofold purpose : firstly , to update the diagnostic approach using the innovative 3-d modalities and secondly , to review the management of this condition during fetal and postnatal life . 
Introduction
Case Report
Discussion