endophytic actinobacteria have a capacity to produce numerous secondary metabolites with a mass of biological activity , such as antibiotics , antitumor and anti - infection agents , plant growth promoters and enzymes , and may promote plant establishment under adverse environmental stresses . introducing such bacterial strains to plant tissues can result in increased plant growth , usually due to suppression of plant pathogenic microorganisms .
it seems to be pivotal for obtaining a healthy microfloral balance within plants , soil appearing to be an important and moderating source of bacterial endophytes .
recently , our group has isolated from a carrot sample from xinjiang uyghur autonomous region ( china ) a novel species of paenibacillus dauci sp .
100608  =  jcm30283 ) , which can produce potential antimicrobial substances playing the part of endophytic actinobacteria . comparisons with 16s rrna gene sequences as shown in fig .
1 revealed that the novel strain had the highest similarity to paenibacillus hunanensis fel05 ( 97% ) .
however , the phylogenetic distances from recognized species ( fig . 2 ) indicated that p. dauci sp . nov . is not affiliated to any of these recognized species .
we can therefore conclude that this strain represents a novel species of the genus paenibacillus . what 's more , high nitrogenase activity , strong antagonism against plant pathogenic fungi , extensive carbon source utilization , and stress resistance were also uncovered . in consequence , investigation of the genetic information and characteristics of p. dauci
knowledge of the genome sequence and bioinformatics will be of great help in this regard . here
we present the draft genome sequence of strain p. dauci h9 obtained using the illumina hiseq 2000 system , which was performed by shenzhen bgi . tech .
the reads were assembled with soapdenovo , , the version is 2.04 , and the sequence was annotated using the rast annotation server ( fig .
sequencing was performed based on the paired - end strategy of 473 reads to produce 790  mb of filtered sequences , representing a 126-fold coverage of the genome .
the sequence of paenibacillus algorifonticola xj259 is 5,449,237 bases with a g  +  c content of 46.5% , which was assembled into 26 contigs and 19 scaffolds .
it contains 4766 open reading frames ( orfs ) , 77 trna genes , and 1 rrna gene ( table 1 ) identified by glimmer 3.02 , genemark , trnascan - se , and rnammer .
according to the genomic analysis of strain p. dauci , we analyzed 36 orfs related to antibiotic metabolic process .
additionally , 12 orfs were also discovered related to trehalose , which makes us believe that it could be related to the shock - resistant mechanism since the trehalose is regarded as a molecular chaperone . what 's more , the biosynthesis of vitamin b was annotated in the strain p. dauci as there were 19 orfs related to vitamin b12 production and vitamin b6 metabolism .
further studies will be performed to confirm their functions , and a complete genome sequence will be included in the future to reveal the unique molecular characteristics of strain p. dauci .
this whole genome shotgun project has been deposited at ddbj / embl / genbank under accession number laqq00000000 .
the version described in this paper is the first version , with accession number laqq01000000 .
the authors declare that there is no conflict of interest on any work published in this paper .
 paenibacillus dauci sp . nov . , 
 a new kind of endophytic actinobacteria , is separated from the inner tissues of carrot sample , which forms intimated associations with carrot acting as biological control agents . 
 here we report a 5.37-mb assembly of its genome sequence and other useful information , including the coding sequences ( cdss ) responsible for biological processes such as antibiotic metabolic process , antimicrobial metabolism , anaerobic regulation and the biosynthesis of vitamin b and polysaccharide . 
 this novel strain can be a potential source of novel lead products for exploitation in the field of pharmaceutical , agriculture and industry . 
Direct link to deposited data
Nucleotide sequence accession numbers
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