in recent years , the results after surgery for rectal cancer in norway , with a 5-year overall survival ( os ) rate of 60.1% , has surpassed that of colon cancer at 57.5% .
this has been achieved because the surgical technique has been standardized according to total mesorectal excision ( tme ) with subsequent dramatic reductions of local recurrences . beginning in 2007 ,
all colon cancers were to be reported separately to the norwegian national cancer registry in an effort to systematically survey and hopefully improve results .
nevertheless , a national strategy to standardize surgical treatment along the lines of radical surgery has neither been implemented in detail nor been generally accepted [ 2 , 3 ] . in this respect ,
the number of lymph nodes retrieved may act as a surrogate measure of radical surgery .
the survival benefit of a large lymph node harvest has been shown in several reports [ 24 ] .
it has been accepted nationally to offer patients with tumor  node  metastasis ( tnm ) stage iii below a certain age , usually 75  years , adjuvant chemotherapy .
it has been decided that a rather arbitrary level of 12 retrieved nodes is enough to obtain adequate surgery and staging .
pathologists may be a key factor for optimal lymph node harvest , and a conjoined effort between surgeon and pathologist would be ideal to improve results [ 26 ] .
the aim of the study was to examine , after modest radical colon surgery removing mesocolic nodes and focus on lymph node yield , what would influence survival and where surgical improvement might be possible using data from a cohort of patients from three large norwegian teaching hospitals .
patients from a national cohort were operated in 2000 , and follow - up was until december 2007 , a mean of 7.5  years later .
three teaching community hospitals , haraldsplass deaconal hospital , stavanger university hospital , and akershus university hospital contributed patients .
all three hospitals are teaching community hospitals , and the patients were operated with an open access by a large number of surgeons . at that time , extra radical surgery was unusual , and it is fair to assume that radical surgery usually constituted a moderate mesocolic resection . if metastases were diagnosed , patients and tumor conditions were assessed regarding feasibility for resection .
patients usually went to the outpatient clinic every third month for the first 2  years and then every sixth month until 5  years had passed .
blood tests with carcino - embryonic antigen measurement and ultrasonography of the liver and chest x - ray were carried out .
elderly patients are stead - bound and even if a few of them were not followed up frequently , they could be tracked and life status ascertained through their identity number in the official national population registry .
the specimen was examined and rinsed by the surgeons on the back table before being mounted on a board and placed in a box filled with enough formaldehyde for secure fixation .
the specimen was examined by a junior pathologist ; after 4872  h , assisted by the consultant .
tissue was paraffin - embedded , and hematoxylin  eosin staining was used routinely before sections were examined microscopically .
metastatic deposits were defined as lymph nodes if these structures resembled nodes but without containing visible lymphatic tissue .
patients younger than 75  years of age that were classified as tnm stage iii were offered 12 courses of adjuvant treatment with 5-fluorouracil plus calsiumfolinate ( flv ) .
the regional committee for medical and health research ethics of western norway and the data inspectorate for national registries approved the study .
the chi - square test was used to compare groups with respect to categorical variables and analysis of variance for continuous variables .
the following variables were analysed with respect to survival : hospital , age , gender , location , no of lymph nodes and lymph node ratio ( lnr ) , t stage , and tnm stage .
survival curves were estimated by the kaplan  meier method   and compared using the log - rank test .
multiple prognostic factors were analysed with the cox proportional hazards model   using the spss 17 package .
all three hospitals are teaching community hospitals , and the patients were operated with an open access by a large number of surgeons . at that time , extra radical surgery was unusual , and it is fair to assume that radical surgery usually constituted a moderate mesocolic resection . if metastases were diagnosed , patients and tumor conditions were assessed regarding feasibility for resection .
patients usually went to the outpatient clinic every third month for the first 2  years and then every sixth month until 5  years had passed .
blood tests with carcino - embryonic antigen measurement and ultrasonography of the liver and chest x - ray were carried out .
elderly patients are stead - bound and even if a few of them were not followed up frequently , they could be tracked and life status ascertained through their identity number in the official national population registry .
the specimen was examined and rinsed by the surgeons on the back table before being mounted on a board and placed in a box filled with enough formaldehyde for secure fixation .
the specimen was examined by a junior pathologist ; after 4872  h , assisted by the consultant .
tissue was paraffin - embedded , and hematoxylin  eosin staining was used routinely before sections were examined microscopically .
metastatic deposits were defined as lymph nodes if these structures resembled nodes but without containing visible lymphatic tissue .
patients younger than 75  years of age that were classified as tnm stage iii were offered 12 courses of adjuvant treatment with 5-fluorouracil plus calsiumfolinate ( flv ) .
the regional committee for medical and health research ethics of western norway and the data inspectorate for national registries approved the study .
the chi - square test was used to compare groups with respect to categorical variables and analysis of variance for continuous variables .
the following variables were analysed with respect to survival : hospital , age , gender , location , no of lymph nodes and lymph node ratio ( lnr ) , t stage , and tnm stage .
survival curves were estimated by the kaplan  meier method   and compared using the log - rank test .
multiple prognostic factors were analysed with the cox proportional hazards model   using the spss 17 package .
two hundred sixty - nine patients , 152 ( 56.5% ) women and 117 men , with a mean age of 71  years ( range , 2093  years ) were studied .
one of the hospitals operated male patients that were younger , with a mean of 67  years .
tumor locations were not different between the hospitals ( p  =  0.059 ) .
locoregional r0 resections for a single tumor location were done in 264 patients , while double resections ( n  =  4 ) and a suspected locoregional r1 resection ( n  =  1 ) were done in five patients . 

table  1tumor locations and pathology variables of patients resected for colon cancer at three teaching community hospitals in 2000variablesall patientschi - square testn  =  269p valuelocation0.059  right hemicolon115 ( 42.8 )  transverse colon including flexures44 ( 16.4 )  descending colon9 ( 3.3 )  sigmoid colon and rectosigmoid96 ( 35.7 )  multiple or r125 ( 1.9)t category0.021  t111 ( 4.1 )  t230 ( 11.2 )  t3180 ( 66.9 )  t448 ( 17.8)tnm stage0.098  i34 ( 12.6 )  ii116 ( 43.1 )  iii93 ( 34.6 )  iv26 ( 9.7)resection of two tumors in separate locationsno radical loco - regional tumor resection tumor locations and pathology variables of patients resected for colon cancer at three teaching community hospitals in 2000 resection of two tumors in separate locations no radical loco - regional tumor resection there were no significant differences between hospitals relating to tnm stage distribution : 34 patients ( 12.6% ) were stage i , 116 patients ( 43.1% ) were stage ii , 93 patients ( 34.6% ) were stage iii , and 26 patients ( 9.6% ) were stage iv .
the number of lymph nodes harvested for various stages were 8.7 ( stage i ) , 10.3 ( stage ii ) , 10.9 ( stage iii ) , and 10.3 ( stage iv ) . in 11 patients ,
the pathologist had classified the t category and tnm stage but omitted to specify the number of lymph nodes present .
twelve or more lymph nodes were examined in 41.1% ( 106/258 ) of the resected specimens . significantly fewer lymph nodes ( p  < 
0.001 ) were harvested at one of the hospitals . otherwise , the three patient populations had similar characteristics .
urgent surgery had a mortality of 12.5% ( 3/24 ) , whereas the elective group had a mortality of 4.5% ( 11/245 ) .
did not differ between the hospitals ( log - rank p  =  0.372 ; fig . 
1kaplan  meier survival curve for 269 patients resected for colon cancer in three norwegian hospitals in 2000 kaplan  meier survival curve for 269 patients resected for colon cancer in three norwegian hospitals in 2000 whether categorizing number of lymph nodes harvested in three groups ( < 10 , 1019 , 20 or more ) or in two ( < 12 , 12 or more ) , no differences was found in os ( log - rank p  =  0.423 and 0.270 , respectively ) .
this was still the case after adjusting for hospital or tnm stage ( log - rank p  =  0.449 ) .
the uncategorized number of lymph nodes was not significant in a simple cox regression ( likelihood ratio p  =  0.129 ) .
however , in one hospital , better survival was found when the lymph node harvest was 12 compared to < 12 ( log - rank p  =  0.037 ) as shown in fig .  2 .
stage ii patients had os of 72.7% with 12 nodes harvested and 58.3% with < 12 nodes
( p  =  0.124 ) , and stage iii patients had a 5-year os of 61.5% and 55.6% , respectively ( p  =  0.508 ) . 

2kaplan  meier survival curve for 103 patients resected for colon cancer in one of three norwegian hospitals in 2000 . patients with a lymph node harvest of 12 or more showed significantly better overall survival ( log - rank test p  =  0.037 ) kaplan  meier survival curve for 103 patients resected for colon cancer in one of three norwegian hospitals in 2000 .
patients with a lymph node harvest of 12 or more showed significantly better overall survival ( log - rank test p  =  0.037 ) in stage iii patients , the lymph node ratio ( lnr ) was highly significant for patient survival , and os for lnr 1 ( < 0.25 ) was 83.3.5% , lnr 2 ( 0.250.50 ) 63.3% , lnr 3 ( 0.510.75 ) 18.8% , and lnr 4 ( 0.761 ) 18.2% ( log - rank test p  <  0.001 ) .
two of the hospitals did more resections of t4 tumors ( chi - square test p  =  0.021 ) .
adjusted for hospital , t4 tumors compared to t1t3 , was a significant adverse factor for survival in the log - rank test ( p  =  0.049 ) . survival according to the different tnm stages is shown in table  2 .
the 5-year os was 58.1% for stage iii and 63.8% for stage ii without differences between the hospitals in uni- and multivariate analyses .
os for stage iv was significantly worse ( p  <  0.001 ) . 

table  2five - year overall survival ( os ) according to the different tnm stages for 269 patients treated with resection for colon cancer in three norwegian teaching community hospitals in 2000tnm stagen ( % ) % osi34 ( 12.6)76.5ii116 ( 43.1)63.8iii93 ( 34.6)58.1iv26 ( 9.7)7.7all stages269 ( 100)58.0 five - year overall survival ( os ) according to the different tnm stages for 269 patients treated with resection for colon cancer in three norwegian teaching community hospitals in 2000 the results of univariate and multivariate cox - regression analyses are shown in table  3 . in the univariate cox regression old age , t category , high lnr , and tnm stage
age as a variable was highly significant both as a continuous variable and with a cutoff value of 69  years .
locations of the tumors with regard to the various resected segments were not significant ( table 3 ) . 

table  3univariate ( n  =  269 patients ) and multivariate ( n  =  258 ) cox regression models for analysis of overall survival for patients resected for colon cancer in three norwegian teaching community hospitals in 2000  univariatemultivariatelr testhr 95% cip valuehr 95% cip valueno . of sampled lymph nodes per increments of 100.79 ( 0.58 , 1.08)0.1290.82 ( 0.57 , 1.17)0.266 t category<0.0010.129  t1t21 ( reference)1 ( reference )  t31.69 ( 0.94 , 3.03)1.84 ( 0.55 , 6.15 )  t43.38 ( 1.79 , 6.40)2.64 ( 0.78 , 8.99)tumor location0.5680.716  right colon1 ( reference)1 ( reference )  transverse colon0.89 ( 0.55 , 1.46)0.79 ( 0.47 , 1.32 )  descending colon0.97 ( 0.39 , 2.42)1.44 ( 0.57 , 3.66 )  sigmoid colon0.70 ( 0.45 , 1.10)0.81 ( 0.50 , 1.31 )  rectosigmoid0.82 ( 0.44 , 1.53)0.62 ( 0.31 , 1.26 )  other / double0.96 ( 0.23 , 3.93)0.67 ( 0.15 , 2.91 )  r25.83 ( 0.79 , 42.81)0.61 ( 0.08 , 4.94)age per 10  years1.46 ( 1.23 , 1.74)0.0011.68 ( 1.38 , 2.03)<0.001gender0.1920.031  females1 ( reference)1 ( reference )  males1.26 ( 0.89 , 1.77)1.52 ( 1.04 , 2.23)tnm stage<0.001<0.001  i1 ( reference)1 ( reference )  ii1.61 ( 0.82 , 3.18)0.91 ( 0.23 , 3.69 )  iii2.07 ( 1.05 , 4.12)1.49 ( 0.39 , 5.66 )  iv10.50 ( 4.94 , 22.32)9.26 ( 2.15 , 39.85)hospital0.3900.169  ahus1 ( reference)1 ( reference )  sus1.06 ( 0.71 , 1.60)0.72 ( 0.46 , 1.14 )  hds1.34 ( 0.87 , 2.07)0.62 ( 0.37 , 1.03)hr hazard ratio ; ci confidence interval ; lr likelihood ratio ; sus stavanger university hospital ; hds haraldsplass deaconal hospital ; ahus akershus university hospitalanalyses based on 258 patients because of lack of specified number of lymph nodesfig . 
3kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to hospital ( log - rank test p  =  0.372 ) univariate ( n  =  269 patients ) and multivariate ( n  =  258 ) cox regression models for analysis of overall survival for patients resected for colon cancer in three norwegian teaching community hospitals in 2000 hr hazard ratio ; ci confidence interval ; lr likelihood ratio ; sus stavanger university hospital ; hds haraldsplass deaconal hospital ; ahus akershus university hospital analyses based on 258 patients because of lack of specified number of lymph nodes kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to hospital ( log - rank test p  =  0.372 ) in the multivariate model age , male gender , high lnr , and tnm stage were adverse factors ( figs .  4 , 5 , and 6 ) . in the fully adjusted multivariate analysis ,
t category was not significant , but when removing tnm stage from the multivariate model , the t category again became significant ( p  =  0.047 ) .
these variables were not significant : hospital , tumor location , or number of harvested lymph nodes in these categories ( < 10 , 1019 , 20 ) .
lnr was also highly significant for stage iii patients ( n  =  93 ) when adjusted for all the variables in table  3 in a multivariate cox regression with hrs ( 95% cis ) of 1.72 ( 0.80 , 3.67 ) , 5.16 ( 2.48 , 10.74 ) , and 4.80 ( 1.92 , 11.99 ) for lnr 24 vs. lnr 1 , respectively ( p  <  0.001 ) . 

4kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to age ( log - rank test p  <  0.001)fig . 
5kaplan  meier survival curves for 93 patients resected for colon cancer in three norwegian hospitals in 2000 according to lymph node ratio groups : < 0.25 ( group 1 ) , 0.250.5 ( group 2 ) , 0.510.75 ( group 3 ) , and > 0.75 ( group 4 ) ( log - rank test p  <  0.001)fig . 
6kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to tnm stage ( log - rank test p 
<  0.001 ) kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to age ( log - rank test p  <  0.001 ) kaplan  meier survival curves for 93 patients resected for colon cancer in three norwegian hospitals in 2000 according to lymph node ratio groups : < 0.25 ( group 1 ) , 0.250.5 ( group 2 ) , 0.510.75 ( group 3 ) , and > 0.75 ( group 4 ) ( log - rank test p  < 
0.001 ) kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to tnm stage ( log - rank test p  <  0.001 ) the study may have been underpowered for detection of an assumed difference between patients with a lymph node harvest of < 12 lymph nodes and those with > 12 . with the number of patients included in our study
, a power of at least 0.84 would be necessary to detect a difference in 5-year survival of 50% vs. 68% . an increase in sample size to detect a difference of 50% vs. 60% with a power of 0.80 in a one - tailed chi - square test
tumor locations were not different between the hospitals ( p  =  0.059 ) .
locoregional r0 resections for a single tumor location were done in 264 patients , while double resections ( n  =  4 ) and a suspected locoregional r1 resection ( n  =  1 ) were done in five patients . 

table  1tumor locations and pathology variables of patients resected for colon cancer at three teaching community hospitals in 2000variablesall patientschi - square testn  =  269p valuelocation0.059  right hemicolon115 ( 42.8 )  transverse colon including flexures44 ( 16.4 )  descending colon9 ( 3.3 )  sigmoid colon and rectosigmoid96 ( 35.7 )  multiple or r125 ( 1.9)t category0.021  t111 ( 4.1 )  t230 ( 11.2 )  t3180 ( 66.9 )  t448 ( 17.8)tnm stage0.098  i34 ( 12.6 )  ii116 ( 43.1 )  iii93 ( 34.6 )  iv26 ( 9.7)resection of two tumors in separate locationsno radical loco - regional tumor resection tumor locations and pathology variables of patients resected for colon cancer at three teaching community hospitals in 2000 resection of two tumors in separate locations no radical loco - regional tumor resection
there were no significant differences between hospitals relating to tnm stage distribution : 34 patients ( 12.6% ) were stage i , 116 patients ( 43.1% ) were stage ii , 93 patients ( 34.6% ) were stage iii , and 26 patients ( 9.6% ) were stage iv .
the number of lymph nodes harvested for various stages were 8.7 ( stage i ) , 10.3 ( stage ii ) , 10.9 ( stage iii ) , and 10.3 ( stage iv ) . in 11 patients ,
the pathologist had classified the t category and tnm stage but omitted to specify the number of lymph nodes present .
twelve or more lymph nodes were examined in 41.1% ( 106/258 ) of the resected specimens .
significantly fewer lymph nodes ( p  <  0.001 ) were harvested at one of the hospitals .
urgent surgery had a mortality of 12.5% ( 3/24 ) , whereas the elective group had a mortality of 4.5% ( 11/245 ) .
the 5-year os was 58.0% ( fig .  1 ) and did not differ between the hospitals ( log - rank p  =  0.372 ; fig . 
1kaplan  meier survival curve for 269 patients resected for colon cancer in three norwegian hospitals in 2000 kaplan  meier survival curve for 269 patients resected for colon cancer in three norwegian hospitals in 2000 whether categorizing number of lymph nodes harvested in three groups ( < 10 , 1019 , 20 or more ) or in two ( < 12 , 12 or more ) , no differences was found in os ( log - rank p  =  0.423 and 0.270 , respectively ) .
this was still the case after adjusting for hospital or tnm stage ( log - rank p  =  0.449 ) .
the uncategorized number of lymph nodes was not significant in a simple cox regression ( likelihood ratio p  =  0.129 ) .
however , in one hospital , better survival was found when the lymph node harvest was 12 compared to < 12 ( log - rank p  =  0.037 ) as shown in fig .  2 .
stage ii patients had os of 72.7% with 12 nodes harvested and 58.3% with < 12 nodes ( p  =  0.124 ) , and stage iii patients had a 5-year os of 61.5% and 55.6% , respectively ( p  =  0.508 ) . 

2kaplan  meier survival curve for 103 patients resected for colon cancer in one of three norwegian hospitals in 2000 .
patients with a lymph node harvest of 12 or more showed significantly better overall survival ( log - rank test p  =  0.037 ) kaplan  meier survival curve for 103 patients resected for colon cancer in one of three norwegian hospitals in 2000 .
patients with a lymph node harvest of 12 or more showed significantly better overall survival ( log - rank test p  =  0.037 ) in stage iii patients , the lymph node ratio ( lnr ) was highly significant for patient survival , and os for lnr 1 ( < 0.25 ) was 83.3.5% , lnr 2 ( 0.250.50 ) 63.3% , lnr 3 ( 0.510.75 ) 18.8% , and lnr 4 ( 0.761 ) 18.2% ( log - rank test p  <  0.001 ) .
two of the hospitals did more resections of t4 tumors ( chi - square test p  =  0.021 ) .
adjusted for hospital , t4 tumors compared to t1t3 , was a significant adverse factor for survival in the log - rank test ( p  =  0.049 ) .
the 5-year os was 58.1% for stage iii and 63.8% for stage ii without differences between the hospitals in uni- and multivariate analyses .
os for stage iv was significantly worse ( p  <  0.001 ) . 

table  2five - year overall survival ( os ) according to the different tnm stages for 269 patients treated with resection for colon cancer in three norwegian teaching community hospitals in 2000tnm stagen ( % ) % osi34 ( 12.6)76.5ii116 ( 43.1)63.8iii93 ( 34.6)58.1iv26 ( 9.7)7.7all stages269 ( 100)58.0 five - year overall survival ( os ) according to the different tnm stages for 269 patients treated with resection for colon cancer in three norwegian teaching community hospitals in 2000 the results of univariate and multivariate cox - regression analyses are shown in table  3 . in the univariate cox regression old age , t category , high lnr , and tnm stage were adverse factors for survival .
age as a variable was highly significant both as a continuous variable and with a cutoff value of 69  years .
locations of the tumors with regard to the various resected segments were not significant ( table 3 )
. 
 table  3univariate ( n  =  269 patients ) and multivariate ( n  =  258 ) cox regression models for analysis of overall survival for patients resected for colon cancer in three norwegian teaching community hospitals in 2000  univariatemultivariatelr testhr 95% cip valuehr 95% cip valueno . of sampled lymph nodes per increments of 100.79 ( 0.58 , 1.08)0.1290.82 ( 0.57 , 1.17)0.266 t category<0.0010.129  t1t21 ( reference)1 ( reference )  t31.69 ( 0.94 , 3.03)1.84 ( 0.55 , 6.15 )  t43.38 ( 1.79 , 6.40)2.64 ( 0.78 , 8.99)tumor location0.5680.716  right colon1 ( reference)1 ( reference )  transverse colon0.89 ( 0.55 , 1.46)0.79 ( 0.47 , 1.32 )  descending colon0.97 ( 0.39 , 2.42)1.44 ( 0.57 , 3.66 )  sigmoid colon0.70 ( 0.45 , 1.10)0.81 ( 0.50 , 1.31 )  rectosigmoid0.82 ( 0.44 , 1.53)0.62 ( 0.31 , 1.26 )  other / double0.96 ( 0.23 , 3.93)0.67 ( 0.15 , 2.91 )  r25.83 ( 0.79 , 42.81)0.61 ( 0.08 , 4.94)age per 10  years1.46 ( 1.23 , 1.74)0.0011.68 ( 1.38 , 2.03)<0.001gender0.1920.031  females1 ( reference)1 ( reference )  males1.26 ( 0.89 , 1.77)1.52 ( 1.04 , 2.23)tnm stage<0.001<0.001  i1 ( reference)1 ( reference )  ii1.61 ( 0.82 , 3.18)0.91 ( 0.23 , 3.69 )  iii2.07 ( 1.05 , 4.12)1.49 ( 0.39 , 5.66 )  iv10.50 ( 4.94 , 22.32)9.26 ( 2.15 , 39.85)hospital0.3900.169  ahus1 ( reference)1 ( reference )  sus1.06 ( 0.71 , 1.60)0.72 ( 0.46 , 1.14 )  hds1.34 ( 0.87 , 2.07)0.62 ( 0.37 , 1.03)hr hazard ratio ; ci confidence interval ; lr likelihood ratio ; sus stavanger university hospital ; hds haraldsplass deaconal hospital ; ahus akershus university hospitalanalyses based on 258 patients because of lack of specified number of lymph nodesfig .  3kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to hospital ( log - rank test p  =  0.372 ) univariate ( n  =  269 patients ) and multivariate ( n  =  258 ) cox regression models for analysis of overall survival for patients resected for colon cancer in three norwegian teaching community hospitals in 2000 hr hazard ratio ; ci confidence interval ; lr likelihood ratio ; sus stavanger university hospital ; hds haraldsplass deaconal hospital ; ahus akershus university hospital analyses based on 258 patients because of lack of specified number of lymph nodes kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to hospital ( log - rank test p  =  0.372 ) in the multivariate model age , male gender , high lnr , and tnm stage were adverse factors ( figs .  4 , 5 , and 6 ) . in the fully adjusted multivariate analysis ,
t category was not significant , but when removing tnm stage from the multivariate model , the t category again became significant ( p  =  0.047 ) .
these variables were not significant : hospital , tumor location , or number of harvested lymph nodes in these categories ( < 10 , 1019 , 20 ) .
lnr was also highly significant for stage iii patients ( n  =  93 ) when adjusted for all the variables in table  3 in a multivariate cox regression with hrs ( 95% cis ) of 1.72 ( 0.80 , 3.67 ) , 5.16 ( 2.48 , 10.74 ) , and 4.80 ( 1.92 , 11.99 ) for lnr 24 vs. lnr 1 , respectively ( p  <  0.001 ) . 

4kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to age ( log - rank test p  <  0.001)fig . 
5kaplan  meier survival curves for 93 patients resected for colon cancer in three norwegian hospitals in 2000 according to lymph node ratio groups : < 0.25 ( group 1 ) , 0.250.5 ( group 2 ) , 0.510.75 ( group 3 ) , and > 0.75 ( group 4 ) ( log - rank test p  <  0.001)fig . 
6kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to tnm stage ( log - rank test p  <  0.001 ) kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to age ( log - rank test p  <  0.001 ) kaplan  meier survival curves for 93 patients resected for colon cancer in three norwegian hospitals in 2000 according to lymph node ratio groups : < 0.25 ( group 1 ) , 0.250.5 ( group 2 ) , 0.510.75 ( group 3 ) , and > 0.75 ( group 4 ) ( log - rank test p  < 
0.001 ) kaplan  meier survival curves for 269 patients resected for colon cancer in three norwegian hospitals in 2000 according to tnm stage ( log - rank test p  <  0.001 )
the study may have been underpowered for detection of an assumed difference between patients with a lymph node harvest of < 12 lymph nodes and those with > 12 . with the number of patients included in our study
, a power of at least 0.84 would be necessary to detect a difference in 5-year survival of 50% vs. 68% . an increase in sample size to detect a difference of 50% vs. 60% with a power of 0.80 in a one - tailed chi - square test
even though several groups have pioneered radical surgery for colon cancer ( complete mesocolic excision and a central tie ) with its potential benefits similar to ( tme ) of rectal cancer surgery , such an approach has not been widely adopted in our country .
therefore , we wanted to examine our background results based on a less standardized approach and potential areas for improvement before embarking on more radical procedures as championed by others .
advanced tnm stage had an adverse influence on outcome , as the results for stage iv versus i  iii and stage iii versus i  ii were significant in the multivariate analysis . for stage iii patients , the 5-year os was 58.1% . in one hospital ,
the 5-year os for stage iii was worse with 47.8% . in the multivariate analysis , however , when adjusted for hospital , there was no significant survival difference .
a recently published international multicenter study of a colorectal population   reported an identical os of 58.8% for patients treated in 20002002 .
others have found a 5-year os of 90.796.3% for stage ii and 64.671.7% for stage iii using radical surgical procedures .
thus , we consider our figures to show room for improvement , although where improvement may be best realised is debatable .
an advanced stage may theoretically be better treated with more radical surgery even though the presence of skip lesions in rare instances may hardly explain this . radical or complete mesocolic excision has been shown to increase both the absolute lymph node harvest and prognosis for the patients [ 2 , 3 ] .
however , it has been contested that patients will benefit from more radical surgery in advanced cases . in a swedish population - based retrospective study ,
a median number of six lymph nodes were detected in the specimens from 1,856 patients operated between 1996 and 2000 .
there were a very low number of nodes in the specimens from the left colon , whereas in the right flexure tumors , the lymph node count was not inferior . in both instances ,
the feeding vessel areas were not divided completely , i.e. , the inferior mesenteric artery proximally and the root of the medial colic artery . in a population - based study from the netherlands by kelder and co - workers , the median lymph node harvest was six and in only 21% of the specimens were 12 or more nodes examined .
thus , we consider that the principle of radical colon surgery may have been violated in these studies when tumors were in those locations . more radical surgery for colon cancer has been recommended in reports from the usa , europe , and japan [ 2 , 15 , 16 ] . a retrospective national report based on a small number with supposedly radical colon surgery found that this approach significantly increased survival compared with a much larger and older group subject to a  standard 
the number of lymph nodes may be seen only as a surrogate marker for the extent of surgery without a proper oncologic explanation for the importance of a large harvest [ 2 , 3 ] .
the node count has been correlated to survival both for stage ii and iii disease [ 2 , 18 ] .
the ontario cancer registry study   showed better survival with a lymph node harvest of more than ten lymph nodes . increasing
in contrast , there is good evidence reported by others that increasing the total number of lymph nodes increases survival significantly [ 2 , 4 ] . in one study , a mean harvest of more than 28 lymph nodes
data from the seer database demonstrated that a cutoff value of 15 was useful , and perhaps , there is an inherent limit to the number of nodes necessary to achieve this effect on survival .
nevertheless , the hypothesis that a larger ( negative ) lymph node yield is beneficial is contradicted even from highly rated institutions .
in the univariate analysis , one of the hospitals achieved a significantly better survival with a harvest of more than 12 lymph nodes .
however , no significant survival benefit was found with harvest of more than 12 lymph nodes in the multivariate analysis .
many pathologists were involved in this trial even though the specimen handling was supposed to be equal . in a recent study ,
the pathologist was found to be the dependent factor in lymph node harvest in multivariate analysis , not the operating surgeon .
the overall poor lymph node harvest may also indicate that more extensive surgery is one way to improve outcome as found in one of the hospitals .
it may be speculated that the poorer outcome in the other two hospitals may have levelled out any benefit by an improved lymph node harvest , i.e. , more radical surgery .
patients below 75  years were given flv chemotherapy , and staging was considered important for that reason . for proper staging of colon cancer ,
the minimum number of lymph nodes needed has been somewhat arbitrarily suggested as 12 by a national cancer institute expert panel .
although some authors [ 14 , 18 ] have concluded with stage migration as an explanation for an increased survival benefit , other studies have shown that detection of positive nodes beyond six or seven lymph nodes examined had no effect on staging [ 19 , 23 ] .
doubtless , a standardized surgical approach in cooperation with a dedicated pathology service is necessary if a minimum number of nodes shall be found and consequently help in outcome assessment .
studies have shown that the lnr is an independent and better marker than pn+ for survival . in our study , lnr was highly significant for os when adjusted for hospitals in the multivariate analysis .
five - year os varied from 83.5% in lnr group 1 , 63.3% in lnr 2 , to 18.8% in lnr group 3 . according to wang et al .
[ 26 , 27 ] , the prognostic effect of lnr did not depend on the total number of lymph nodes nor on the number of positive nodes .
in contrast , berger et al .   found that lnr was not a significant prognostic indicator with less than ten examined nodes but became highly significant for os and disease - free survival with more than ten lymph nodes harvested .
a mayo clinic study   found a positive correlation between the number of positive lymph nodes and survival but did not analyze the effect of lnr .
fifty - eight percent of our stage iii patients had less than 12 lymph nodes examined .
still , we found a significant survival difference of 44.5% between lnr group 2 and 3 and 65.3% between group 1 and 4 .
a study from new zealand   found that both the absolute number of lymph nodes retrieved up to 16 , as well as the lnr , were important for prognosis .
the share of stage iii compared to stage ii cancers in their patients amounted to 54.9% , higher than in most other reports .
it seems obvious that a large negative lymph node yield will down - regulate lnr , but the importance of this with regard to outcome may need to be examined in more detail .
another national report of colon cancer from a single institution found that an emergency operation , some colon locations , blood transfusion of more than two units , old age , and tnm stage were negative predictive factors .
we did not study the effect of blood transfusion , and emergency operations were not separated from the rest partly because they were few , i.e. , 15% reported by sjo et al . , and the definition may be debatable .
the different colon locations found to be statistically significant ( transverse , left flexure , and descending colon ) were not so in our multivariate analysis . we suspect that this is partly because they were too few , 20% , to make an impact .
even though the number of urgently operated patients was small in this series , as expected , this small group had a higher mortality of 12.5% compared to 4.5% in the elective patients .
severe co - morbidities in old age may have contributed to these figures as has been reported by others .
however , it should be possible to set an optimal target of < 3% in elective cases .
the average age of our study population was 71  years and that may be older than in series of selected patients .
the average age of men and women in our country for those that have reached the age of 62 ( earliest oap retirement age ) is 81 and 85  years . to circumvent the analytic problem of age , sjo et al
thus , their survival figures improved from 62% actuarial survival to 74% for women and 79% for men .
the method is cumbersome , as it necessitates life tables to calculate this for every patient year .
three - year disease - free survival has been shown to parallel overall 5-year survival .
however , it requires close follow - up with ct instead of the conventional ultrasonography and chest x - ray examinations .
close follow - up may even result in better treatment of metastases according to japanese results .
only 41% had a lymph node harvest of 12 or more lymph nodes , and although this influenced os in one hospital , it did not overall .
more radical surgery may increase the lymph node yield , but if this has the potential to increase overall survival in our patients remains to be seen in the prospective registry .
mortality should be kept low with adequate assessment and treatment of co - morbidities as well as meticulous surgery to avoid complications .
 backgrounda national surveillance program of colon cancer treatment was introduced in 2007 . 
 we examined prognostic factors for colon cancer operated in 2000 with an aim of improving survival in the new program and a special focus on the merit of lymph node yield.methodsa cohort of 269 patients , 152 women ( 56.5% ) , with a mean age of 71  years , was operated for colon cancer in 2000 at three teaching hospitals and followed up for 7  years.resultsoverall 5-year survival was 58.0% , and overall hospital mortality was 5.2% , with 4.5% in elective cases and 12.5% after urgent surgery . in only 41.1% of the specimens were 12 or more lymph nodes retrieved , but this did not affect survival in the combined cohort , although one of the hospitals achieved a significantly better result with a harvest of 12 or more lymph nodes . in a multivariate analysis , old age , 
 gender , a high lymph node ratio ( lnr ) at stage iii , and tumor  node  metastasis stage were adverse factors for survival.conclusionsthe operative mortality was high and should be reassessed . 
 the lymph node count did not have a significant impact on outcome overall , whereas the lnr proved significant for stage iii . a prospective protocol using overall lymph node yield as a surrogate measure for more radical surgery 
 , nevertheless , seems warranted to improve the lymph node harvest according to international recommendations . 
Introduction
Material and methods
Surgery
Follow-up
Pathology
Oncology
Ethics
Statistical analysis
Results
Surgery
Pathology
Survival analysis
Statistical analysis
Discussion
Conclusion