
 escherichia coli o157:h7 is an enterohemorrhagic strain of   e. coli   that produces a powerful shiga - like toxin .
it is capable of causing bloody stools , hemorrhagic colitis , and hemolytic uremic syndrome .
most outbreaks have been associated with the consumption of contaminated , undercooked , bovine food products .
there also have been reports of e. coli o157:h7 outbreaks associated with both drinking and recreational water [ 37 ] . 

e. coli o157:h7 is ubiquitous on farms where healthy cattle and sheep harbor the pathogen in their gastrointestinal tracts . as a consequence ,
farm animal manure is a source for spreading e. coli o157:h7 into the environment and potentially to the human food chain .
one of the most common modes by which e. coli o157:h7 is introduced onto food crops is through contaminated irrigation water .
in addition , the propagation of this pathogen through the environment has been linked to runoff contaminated with bovine manure or by use as soil amendment .
the contamination of surface and ground water in rural areas of the united states is becoming increasingly more common as a result of concentrated animal feeding operations .   

escherichia coli o157:h7 can survive in varying habitats under a wide range of conditions . in the environment
, cells are exposed to rapidly changing conditions such as changes in ph , nutrient availability , temperature , oxidative stress , and osmotic challenge .
e. coli o157:h7 pollution of water and soil is dependent on the ability of this pathogen to adapt to these changes .
however , there is limited information concerning the survival of e. coli o157:h7 in soil and water as growth of bacteria , if any , in these environments is not well understood or documented .
e. coli o157:h7 may respond to adverse conditions in the environment by expressing various stress response genes that enable survival .
the master regulator of the general stress response is an alternative sigma factor  ( rpos ) .  
this sigma factor may be induced in response to stresses such as weak acids , starvation , high osmolarity , and high or low temperature .  
there is evidence that stress responses may enable survival under more severe conditions , increase pathogenicity , and enhance resistance to secondary stresses . in other words ,
when e. coli cells are stressed , they become harder to kill and are more resistant to starvation and toxic chemicals typically used in distribution systems such as chlorine .
this has significant public health implications because e. coli o157:h7 could develop a disinfectant - resistant phenotype during transport to water treatment plants .
therefore , understanding the effects of stress on gene expression in response to altered environmental conditions may be crucial in understanding the survival of this organism as it moves from one environment to another .   
limited work has been conducted to investigate e. coli o157:h7 survival and functional genomics in the environment .
further research is needed to understand the mechanisms that enable e. coli o157:h7 to survive in such a wide variety of environments .
this study compared genetic expression profiles of escherichia coli o157:h7 under two environmental conditions ( soil and natural water ) to expression in growth media using dna microarrays .  
in addition , we investigated the long - term survival of e. coli o157:h7 in microcosms simulating these environments .
soil and water were collected from newby ditch in june of 2006 within 24 hours of a rainfall event ( 1.93  cm or 0.76 inches ) .
newby ditch is located within the mississinewa watershed on the tipton till plain of east central indiana ( usa ) .
environmental samples were placed in sterile bottles and stored in a cooler with ice packs for transport to the lab .  
the samples were processed within 24 hours for use as soil and water microcosms according to the methods described below . 

e. coli o157:h7 strain atcc 35150 was used to investigate survival and differences in gene expression under two different environmental conditions compared to luria broth ( lb ) ( bd , rockville , md ) .   for long - term storage , the culture
was maintained in 10% glycerol at 50c .   in the short term , cells were cultured on nutrient agar ( bd ) slants at 4c .  
the e. coli o157:h7 experiment cultures were prepared by transferring a loopful of a slant culture into a 100  ml flask containing 30  ml of lb .  
this culture was used to inoculate the three treatment conditions : 30  ml lb control , 90  ml sterile stream water microcosms , and 100-g sterile soil microcosms .
the 30  ml lb control ( 100  ml flask ) was inoculated with 1  ml of the culture and incubated at 15c for 48 hours with shaking at 60  rpm .
the remaining cells were harvested by centrifugation at 10,000    g for 10 minutes at 4c ( sorvall rc-5b ) , washed twice with sterile deionized water , and resuspended in 10  ml of sterile stream water or 10  ml of sterile deionized water .
the control and water experiments were performed using ten replicates each , four for microarray analysis and six to monitor survival with replacement .  
the soil treatment was prepared with 34 replicates , four for microarray analysis and 30 to monitor survival without replacement .
the water microcosms were incubated at 15c with shaking at 60  rpm , and the soil microcosms were incubated at 15c without shaking .  
four microcosms , each of soil and water , were removed after 14 days for dna microarray analysis .  
four flasks of the 15c lb control were removed after 48 hours for dna microarray analysis .
aliquots of water ( 90  ml ) were placed into 250  ml sterile bottles and autoclaved at 121c at 15  psi for 15 minutes . following sterilization ,
the water microcosms were stored at 4c until inoculation . following inoculation with 8.8  10  cfu / ml of e. coli o157:h7 atcc 35150
the soil used in this study was a fox silt loam with a ph of 6.95 .
it is a well - drained soil with a moderate available water capacity , medium runoff , and poor filtering capacity .
soil ph was determined by placing 20  g of soil into a 50  ml beaker , adding 20  ml of deionized water , and stirring for 30 minutes .
this suspension was allowed to settle for an hour , and ph was measured with a ph meter .
soil moisture content was determined using the gravimetric method ; soil was weighed , oven dried at 105c , and then reweighed until the sample weight was constant .
the soil moisture content was determined to be 0.323 ( 32.3% ) , which is a slightly moist soil .  
large rocks and debris were removed from the dried soil , and 100-g aliquots were placed in 250  ml glass bottles .
the soil microcosms were autoclaved at 121c at 15  psi for 15 minutes following sterilization , the soil was returned to the drying oven for 24 hours at 105c to remove any residual moisture .
sterile soil , as checked by plate counting , was stored at room temperature prior to inoculation .
soil moisture content was adjusted to field conditions by adding 32.3  ml of sterile deionized water . following inoculation with 8.8  10  cfu / ml of e. coli o157:h7 atcc 35150
the initial ( zero - time postinoculation ) concentration e. coli o157:h7 was determined for each condition prior to incubation .
e. coli o157:h7 concentrations were determined for each condition by using the spread plate method .  
for extraction of bacteria from soil , 100  ml 0.1% polyethylene glycol ( peg ) was added to 100  g of soil .
this suspension was shaken ( 150  rpm ) for 15 minutes at 15c and then allowed to settle for five minutes .
the supernatant from this suspension was centrifuged for ten minutes ( 10,000    g , 4c ) , and the resulting supernatant was used to enumerate the cells .
viable cell counts were performed in duplicate by serial dilution in 0.9% sterile saline and spread plate culturing 0.1  ml onto r2a agar ( bd ) plates .
the plates were incubated at 37c for 48 hours . following incubation , plates with colony counts between 25 and 250
the average number of colonies was divided by the volume or mass of the original solution to estimate the number of cfu / ml and cfu / g , respectively .
total rna was isolated using the fastrna pro soil - direct kit ( qbiogene , inc . , ca ) with minor modifications ( described below ) to improve quality and yield .  
the kit - supplied lysing matrix e tubes were placed at 10c for 2 days prior to rna extraction to minimize heating and rna degradation .  
one gram of the sample was placed into the kit - supplied lysing matrix e tubes for lysis .
sample lysis was performed using a mini - beadbeater-1 instrument ( biospec products , inc . ,
bartlesville , ok ) for 80 seconds at a speed setting of 48 ( maximum speed ) .
rna was centrifuged through the kit - supplied quick - clean spin filters to remove residual inhibitors following extraction and stored in 100  l of depc - h20 at 50c .  
rna quality was determined by 1.0% ( w / v ) agarose gel electrophoresis and by spectrophotometric analysis ( od260/od280 ) using a thermo scientific spectronic genesys 5 spectrophotometer .  
howard edenberg 's laboratory at the indiana university school of medicine center for medical genomics where the microarray analysis was performed .
dna microarrays were used to evaluate the genetic expression profiles of e. coli o157:h7 atcc 35150 maintained under three environmental conditions .  
the microarray system used for e. coli was the genechip e. coli genome 2.0 array ( affymetrix , inc . ,
the microarray analysis was performed with four biologically - independent replicates ( with respect to e. coli growth , rna isolation , sample preparation , and array hybridization ) for each treatment condition .    the standard protocol for prokaryotic sample and array processing recommended by affymetrix in their genechip expression analysis technical manual ( affymetrix , santa clara , ca ) was used .
cdna was synthesized using a t7 promoter - dt24 oligonucleotide as a primer with the invitrogen life technologies superscrip choice system .  
following second - strand cdna synthesis and incubation with t4 dna polymerase , the products were purified using the affymetrix cleanup module .   biotinylated crna was made using the affymetrix ivt kit .  
the crna was purified using the qiagen rneasy column , quantitated , and then fragmented by incubation at high temperature with magnesium .
biotinylated crna was then added to a hybridization solution and hybridized to the genechip after adding control oligonucleotides at 45c for 17 hours with constant rotation
. the hybridization mixture was removed , and the genechip was washed and stained with phycoerythrin - labeled streptavidin using the affymetrix fluidics station . the genechip was washed again , incubated with biotinylated antistreptavidin , and then restained with phycoerythrin - labeled streptavidin to amplify the signals .
the microarray expression data were generated using affymetrix gcos software . the affymetrix microarray suite algorithm was used to analyze the hybridization intensity data from genechip expression probe arrays and to calculate a set of metrics to describe probe set performance .
the average intensity of each array was normalized by global scaling to a target intensity of 1000 .
an average expression value for each treatment group was calculated via geometric mean because it is better applied to data with large fluctuations .
two treatments were compared by determining the log  2 ratio of gene expression for the corresponding averaged intensities for each treatment .
fold change was calculated from log  2 data such that values for induction range from 1 to 100 while values for repression are restricted to the space between 0 and 1 .
for example , a value of 2 indicates a 2-fold upregulation while a value of 0.5 indicates a 2-fold downregulation for a gene comparing the environmental condition to the luria broth .
a t - test on the log  2 transformed data was performed using microsoft excel .
significant gene selection was performed using the microsoft excel filter function to select for genes with greater than or equal to a 2-fold up- or downregulation and a p - value less than .05 .   
functions of significantly expressed genes were determined using the affymetrixs netaffx analysis center ( http://www.affymetrix.com/analysis/netaffx/index.affx ) and ecocyc ( http://ecocyc.org/ ) database .  
soil microcosms were inoculated with 10 ml of 8.8    10  cfu / g of e. coli o157:h7 strain atcc 35150 .   immediately after inoculation ,
the average preincubation concentration of e. coli o157:h7 was 1.8    10  cfu / g .  
following incubation at 15c , survival was monitored on a regular basis for 179 days .
no significant decrease in the e. coli o157:h7 concentration was observed during the first 30 days of incubation ( figure 1 ) .
the last measurement was taken on day 179 , and the average concentration of cells was 7.7  10  cfu / g ( data not shown ) .
sterile water was inoculated with 10  ml of 8.8  10  cfu / ml of e. coli o157:h7 strain atcc 35150 . on day 0 ,
the mean concentration of e. coli o157:h7 was 1.1  10  cfu / ml .
the microcosms were incubated at 15c with gentle shaking at 60  rpm , and survival was monitored for 234 days . a decrease in the e. coli o157:h7 concentration below the postinoculation concentration
the final measurement was taken on day 234 ( nearly 8 months following inoculation ) , and the mean concentration of cells was 2.98  10  cfu / ml ( data not shown ) .  
the genomic expression profiles of e. coli o157:h7 atcc 35150 incubated in sterile soil , water , and lb were evaluated .
the log expression ratios of the e. coli o157:h7 genome for cells grown in lb at 15c for 48 hours with shaking at 60  rpm versus fourteen - day incubation in sterile stream water at 15c are illustrated in figure 3 .
the whole genome analysis indicated that 705 genes were more highly expressed in lb ( plotted with a negative value ) while 751 genes were more highly expressed in sterile stream water ( plotted with a positive value ) ( figure 3 ) .
figure 4 shows the log expression ratios of the e. coli o157:h7 genome for cells grown in lb at 15c for 48 hours versus fourteen - day incubation in sterile soil at 15c .
the analysis of the whole genome indicated that 2,664 genes were more highly expressed in lb ( plotted with a negative value ) while 1,823 genes were more highly expressed in sterile soil ( plotted with a positive value ) .  
an analysis of gene ratios with significant expression levels (  2 folds and p - value <  .05 ) revealed that the majority of genes did not differ significantly between conditions .
this was especially true of the cells grown in lb compared with the cells incubated in sterile stream water ; 26 genes were more significantly expressed in lb compared to 12 genes in cells incubated in sterile stream water ( table 1 ) .
the comparison of cells incubated in sterile soil compared to cells grown in lb yielded more differences in expression ; 89 genes were expressed at significantly higher levels in lb while 308 genes were more highly expressed in sterile soil ( table 2 ) .   
a functional group analysis was performed for significantly expressed genes in lb versus sterile soil ( table 2 ) and lb versus sterile stream water ( table 1 ) .
functional group analysis of lb versus sterile soil revealed that cells incubated in sterile soil expressed more genes for antibiotic resistance , biosynthesis , dna replication / repair and restriction / modification , metabolism , pathogenesis and virulence , phages , transposons , and plasmids , ribosomal proteins , stress response , transcription , rna processing , and degradation , translation and posttranslational modification , and transport and binding proteins ( table 2 ) .
functional group analysis of lb versus sterile stream water revealed that cells incubated in sterile stream water expressed more genes of unknown function while cells grown in lb expressed more genes for ribosomal proteins , signaling and motility , stress response , transcription , rna processing , and degradation , translation and posttranslational modification , and transport and binding proteins ( table 1 ) .
the nature of these differences in genomic expression is described in detail in tables 3 and 4 .
for example , seven of the genes for amino acid biosynthesis were expressed at significantly higher levels in soil than in lb ( table 3 ) .
there were no differences in expression of these amino acid biosynthesis genes between cells grown in lb and cells incubated in sterile natural water ( table 4 ) .
of the 55 genes that are known to encode ribosomal proteins , 45 were expressed at significantly higher levels in soil compared to luria broth ( table 2 ) . only one ribosomal protein gene ( rpmc )
was significantly expressed in cells grown in lb compared to water ( table 1 ) .  
the genes responsible for the stress response include those that function in temperature shock , acid tolerance , the sos response , and osmotic challenge .
eighteen stress response genes were significantly expressed in cells incubated in sterile soil compared to lb ( table 2 ) . on the other hand ,
three stress response genes were more significantly expressed in lb compared to cells incubated in sterile natural water ( table 1 ) .
the rpos gene is induced in response to entry into stationary phase and also by stresses such as weak acids , starvation , osmotic challenge , and temperature changes .
the expression of rpos was significantly elevated in soil ( 2.68-fold induction ) ( table 3 ) .
the rpoh heat shock sigma factor 32 (  ) , which regulates the heat shock response , was more highly expressed in soil compared to lb ( 3.19-fold induction ) ( table 3 ) .
cells grown in soil expressed heat shock genes dnak and htpx at significantly greater levels ( table 3 ) .
in addition , table 3 shows that rsea , an antisigma regulator of the rpoe envelope heat stress system , was induced in cells incubated in soil ( 3.29-fold induction ) .
numerous cold shock genes were significantly expressed in cells incubated in soil compared to cells grown in lb :   cspa , cspe , cspg , ymce , dead , yfia ( table 3 ) .
only one cold shock gene ( cspc ) was expressed at significantly lower levels in cells incubated in sterile water compared to cells grown in lb ( 0.48-fold repression ) ( table 4 ) .
two genes involved in the sos response were significantly expressed in cells grown in soil compared to lb :   reca and sula .
the osmotically inducible gene osmb was expressed at significantly higher levels in cells incubated in soil ( 4.77-fold induction ) .
seven genes that aid in pathogenesis and virulence were significantly expressed in cells incubated in soil compared to those grown in lb ( table 2 ) . in particular , the vacb gene was expressed ( 2.26-fold induction ) ( table 3 ) .
tola , a gene involved in colicin production , was significantly expressed in cells incubated in soil compared to cells grown in lb ( 2.26-fold induction ) ( table 3 ) .
also , the soxs gene was more highly expressed in cells incubated in sterile soil microcosms ( 2.44-fold induction ) ( table 3 ) .
finally , three antibiotic resistance genes ( marr , mara , and marb ) were expressed at significantly higher levels in cells incubated in sterile soil microcosms compared to lb ( table 2 ) .

 e. coli o157:h7 may encounter conditions that are less than optimal for growth in soil and water and must adapt to these conditions in order to survive .
various stress response mechanisms allow this pathogen to adapt to sublethal environmental conditions .   extended exposure to these stresses enables e. coli o157:h7 to survive under more severe conditions , increases its pathogenesis , and enhances its resistance to chemicals typically used in water distribution systems .
this has significant public health implications because e. coli o157:h7 could develop a disinfectant - resistant phenotype during transport to water treatment plants .
therefore , this study investigated the survival and genetic expression profiles of e. coli o157:h7 in sterile soil and sterile natural water .
our results indicate that e. coli o157:h7 can persist for long periods of time in sterile soil and sterile stream water .  
in addition , we found that e. coli o157:h7 exhibits differential gene expression profiles in sterile soil and sterile stream water compared to cells freshly grown in lb .
this survival does not account for the possible effects of competition with other bacteria or interactions with predatory protozoa . under natural conditions , where predators and other bacteria are present ,
it is also possible that the environmental persistence of e. coli o157:h7 cells initially grown in fecal extracts could be different .
microarray analysis revealed that cells incubated in sterile soil for 14 days remain very active .
in fact , 308 genes were found to be more highly expressed in these cells compared to cells grown in lb .
a functional group analysis revealed that the majority of these genes were involved in amino acid biosynthesis , dna replication and repair , pathogenesis and virulence , the stress response , ribosomal proteins , antibiotic resistance , transcription , and translation . on the other hand , microarray analysis of cells placed in sterile stream water for 14 days
typically , faster - growing cells synthesize proteins more rapidly and contain more ribosomes [ 19 , 20 ] .
these researchers found that 42 ribosomal protein genes were expressed at significantly higher levels in cells grown under high nutrient conditions .
the present study , on the other hand , revealed that 45 ribosomal protein genes were more highly expressed in cells incubated in sterile soil compared to cells grown in lb .
the exception to growth - rate - dependent regulation of ribosome number occurs at very low growth rates .
when e. coli cells adjust to a slow growth rate from a fast one , rna accumulation is attenuated for a short time until the rna content is reduced to that characteristic of cells grown at the slower rate .   
it is thought that the same mechanism that functions during amino acid starvation also functions during growth rate transitions .
in fact , the continued accumulation of rna in cells under partial amino acid starvation has been shown to be accompanied by a continued synthesis in ribosomal proteins .
this could account for the higher expression of genes for ribosomal proteins observed in the present study as genes encoding the enzymes needed for amino acid biosynthesis were more highly expressed in cells incubated in sterile soil microcosms . in e. coli , there are 97 known genes responsible for encoding the enzymes needed for amino acid biosynthesis .
previous results   indicate that these genes are induced for growth in low nutrient environments as appears to be the case in the present study .
growth conditions that lead to a decreased rate of ribosome synthesis typically result in an excess of ribosomal proteins , and their transcript levels are higher in faster growing cells .    
the regulatory mechanism that controls the general stress response is the rpos sigma factor (  ) and is encoded by the rpos gene .
this gene , which controls the expression of more than 50 proteins , is induced in response to entry into stationary phase and also by stresses such as weak acids , starvation , osmotic challenge , and temperature changes .
the expression of rpos in cells in sterile soil microcosms was 2.68-fold higher when compared to cells cultured in luria broth .
in addition , the expression of 18 genes involved in the stress response was more highly expressed in cells from soil .  
these genes regulate cellular response to cold shock , heat shock , acid tolerance , osmotic challenge , and the sos response .
this indicates that the soil environment stressed these cells , and they turned on genes to cope with sublethal environmental conditions .     
the heat shock response is a protective mechanism to cope with heat - induced damage to proteins ; however , there is evidence suggesting that these genes are also induced in response to acidic conditions , sos - inducing treatments , and sublethal exposure to chlorine .
most heat shock proteins act as molecular chaperones that bind to and stabilize unfolded proteins and promote protein refolding and proper assembly .  
the dnak gene product has been shown to regulate other heat shock proteins , such as htpx , and play a major role in digesting irreversibly heat damaged polypeptides .
in addition to heath shock proteins , numerous cold shock genes were significantly expressed in cells incubated in soil compared to cells grown in lb : cspa , cspe , cspg , ymce , dead , yfia .
a specific sigma factor has not yet been identified in the case of the cold shock response .
two genes involved in the sos response were significantly expressed in cells grown in soil compared to lb :   reca and sula .
the reca protein functions as a positive control for sos regulation , is required for all homologous recombination in e. coli , and catalyzes synapsis and strand exchange between homologous molecules .
when cells are exposed to sos - inducing environments , they will continue to elongate but fail to septate and thus form filaments .  
several genes responsible for the pathogenesis and virulence of e. coli o157:h7 were significantly expressed in cells from sterile soil microcosms .
the vacb gene , which is required for the full expression of the virulence phenotype in e. coli , was highly expressed in cells incubated in soil . moreover , a gene involved in colonic acid biosynthesis , wcal , was more highly expressed in soil compared to lb .  
colanic acid forms a protective capsule around the bacterial cell surface and plays a role in pathogenesis .
.   demonstrated that colonic acid synthesis is upregulated in biofilms and is not synthesized in planktonic cells under normal laboratory conditions .
this may account for the differences in colonic acid gene expression observed between the cells incubated in sterile soil microcosms ( in which biofilms are likely ) compared to the lb control .
a gene involved in colicin production , tola , was significantly expressed at a higher level in cells incubated in soil compared to cells grown in luria broth .
colicins are antibacterial proteins produced by some strains of e. coli that kill competing strains of bacteria by inhibiting energy metabolism , protein synthesis , or dna synthesis .
in addition , three genes ( marr , mara , and marb ) responsible for multiple antibiotic resistance were more highly expressed in sterile soil .   the multiple antibiotic resistance ( mar ) locus in e. coli is composed of two operons ( marc and marrab ) .
moreover , the elevated expression of the soxs gene product has been associated with the multiple antibiotic resistance ( mar ) phenotype .
the collective expression of these genes and the genes involved in the general stress response may contribute to bacterial survival and virulence during infection .
in fact , there is evidence that antibiotic treatment increases the development of hemolytic uremic syndrome ( hus ) in children with e. coli o157:h7 infection .    in conclusion ,
affymetrix genechip e. coli genome arrays were used to demonstrate that e. coli o157:h7 cells placed in sterile soil and water microcosms at 15c for 14 days exhibit differential gene expression compared to cells grown in lb at 15c for 48 hours .
the cells incubated in sterile soil microcosms were undoubtedly stressed and therefore in a different physiological state than cells grown in lb at 15c for 48 hours .
these cells exhibit a phenotype that may lead to stress - associated disinfection resistance , increased pathogenesis , and virulence .
this has important implications in water treatment and public health because surface and ground waters are the source for municipal drinking water .
further research on the mechanisms and regulation of the stress response of e. coli o157:h7 is needed to prevent potential risk of disease .
it is also possible that the genetics expression could be different in nonsterile environments , and this needs to be investigated .
 the in vitro survival of escherichia coli o157:h7 at 15c 
  under two experimental conditions ( sterile soil and sterile natural water ) was examined . 
 dna microarrays of the entire set of e. coli o157:h7 genes were used to measure the genomic expression patterns after 14 days . 
 although the populations declined , some e. coli o157:h7 cells survived in sterile stream water up to 234 days and in sterile soil for up to 179 days . 
 cells incubated in soil microcosms for 14 days expressed genes for antibiotic resistance , biosynthesis , dna replication and modification , metabolism , phages , transposons , plasmids , pathogenesis and virulence , antibiotic resistance , ribosomal proteins , the stress response , transcription , translation , and transport and binding proteins at significantly higher levels than cells grown in luria broth . 
 these results suggest that e. coli o157:h7 may develop a different phenotype during transport through the environment . 
 furthermore , this pathogen may become more resistant to antibiotics making subsequent infections more difficult to treat . 
1. Introduction
2. Materials and Methods
3. Results
4. Discussion