this study was approved by the local ethics committee , and each patient gave informed consent for participation .
the clinical diagnosis of pd was made according to the uk brain bank criteria.11 at the time of the study , none of the patients had ever taken medications for pd .
all patients underwent a detailed clinical evaluation , including laboratory tests for lipid profiles and serum homocysteine . at the time of study enrollment , all included patients
were evaluated using the unified parkinson s disease rating scale ( updrs ) and the modified hoehn and yahr staging score .
patients fasted the night before the test , except for a small amount of water , if they were thirsty and abstained from drinking alcohol or coffee the day before the study .
continuous electrocardiographic and non - invasive blood pressure monitoring was used in each patient ( ym6000 , mediana tech . , san antonio , tx , usa ) . after 30 min of supine rest
, the test ( 20 min at 60 ) was performed using the manumed special tilt1-section ( enraf nonius , rotterdam , the netherlands ) . by consensus ,
orthostatic hypotension was defined as a fall in blood pressure of at least 20 mm hg systolic pressure and/or 10 mm hg diastolic pressure between 2 and 5 minutes after tilt.12 all patients enrolled in the study underwent conventional mri with a 3.0-t system ( magnetom verio , 3 t , siemens ) .
axial t2 weighted images , complemented with coronal fluid attenuated recovery images , were used to rate the wmh .
the mr images were rated using the semiquantitative visual rating system of scheltens et al.13 this rating scale provides four sum scores in a semiquantitative way ; periventricular hyperintensities ( 06 ) , deep wmh ( 024 ) , basal ganglia hyperintensities ( 030 ) , and infratentorial hyperintensities ( 030 ) .
periventricular hyperintensities were identified as continuous , confluent areas of high signal intensity adjacent to the anterior or posterior horns of the lateral ventricles (  cap  ) and along the lateral ventricles (  bands  ) .
absence of lesions was a score of 0 ,  5 mm lesions was a score of 1 , and a score of 2 was given for lesions > 5 mm .
wmh , located in the deep and subcortical white matter , were separately rated in the frontal , temporal , parietal , and occipital regions according to the previously described criteria.13 these rating criteria were also applied to regions of the basal ganglia ( caudate , putamen , globus pallidus , thalamus , and internal capsule ) and infratentorial regions ( cerebellum , midbrain , pons , and medulla ) . the independent sample t - test or pearson s
chi - square test was used to compare the differences between the groups categorized by the blood pressure response during orthostasis ( non - oh and oh ) . given the potential influence of several variables on the results of wmh , we also conducted group comparisons with an analysis of covariance ( ancova ) including age , the presence of hypertension and diabetes , low density lipoprotein ( ldl)-cholesterol , and serum homocysteine levels as confounding covariates .
patients were also divided into no to mild and severe wmhs groups based on two extremes by tertile distribution .
the relationship between blood pressure profiles monitored during tilt table testing tilt and wmh were tested using spearman s rank correlation coefficients .
the statistical analysis was performed with spss software version 15.0 ( spss , inc . ,
this study was approved by the local ethics committee , and each patient gave informed consent for participation .
the clinical diagnosis of pd was made according to the uk brain bank criteria.11 at the time of the study , none of the patients had ever taken medications for pd .
all patients underwent a detailed clinical evaluation , including laboratory tests for lipid profiles and serum homocysteine . at the time of study enrollment , all included patients
were evaluated using the unified parkinson s disease rating scale ( updrs ) and the modified hoehn and yahr staging score .
patients fasted the night before the test , except for a small amount of water , if they were thirsty and abstained from drinking alcohol or coffee the day before the study .
continuous electrocardiographic and non - invasive blood pressure monitoring was used in each patient ( ym6000 , mediana tech . , san antonio , tx , usa ) .
after 30 min of supine rest , the test ( 20 min at 60 ) was performed using the manumed special tilt1-section ( enraf nonius , rotterdam , the netherlands ) . by consensus ,
orthostatic hypotension was defined as a fall in blood pressure of at least 20 mm hg systolic pressure and/or 10 mm hg diastolic pressure between 2 and 5 minutes after tilt.12
all patients enrolled in the study underwent conventional mri with a 3.0-t system ( magnetom verio , 3 t , siemens ) .
axial t2 weighted images , complemented with coronal fluid attenuated recovery images , were used to rate the wmh .
the mr images were rated using the semiquantitative visual rating system of scheltens et al.13 this rating scale provides four sum scores in a semiquantitative way ; periventricular hyperintensities ( 06 ) , deep wmh ( 024 ) , basal ganglia hyperintensities ( 030 ) , and infratentorial hyperintensities ( 030 ) .
periventricular hyperintensities were identified as continuous , confluent areas of high signal intensity adjacent to the anterior or posterior horns of the lateral ventricles (  cap  ) and along the lateral ventricles (  bands  ) .
absence of lesions was a score of 0 ,  5 mm lesions was a score of 1 , and a score of 2 was given for lesions > 5 mm .
wmh , located in the deep and subcortical white matter , were separately rated in the frontal , temporal , parietal , and occipital regions according to the previously described criteria.13 these rating criteria were also applied to regions of the basal ganglia ( caudate , putamen , globus pallidus , thalamus , and internal capsule ) and infratentorial regions ( cerebellum , midbrain , pons , and medulla ) .
the independent sample t - test or pearson s chi - square test was used to compare the differences between the groups categorized by the blood pressure response during orthostasis ( non - oh and oh ) . given the potential influence of several variables on the results of wmh , we also conducted group comparisons with an analysis of covariance ( ancova ) including age , the presence of hypertension and diabetes , low density lipoprotein ( ldl)-cholesterol , and serum homocysteine levels as confounding covariates .
patients were also divided into no to mild and severe wmhs groups based on two extremes by tertile distribution .
the relationship between blood pressure profiles monitored during tilt table testing tilt and wmh were tested using spearman s rank correlation coefficients .
the statistical analysis was performed with spss software version 15.0 ( spss , inc . ,
among the 117 patients enrolled , 46 were men and 71 were women . the mean age at examination was 68.5  10.1 years , and the mean duration of motor symptoms was 2.1  1.0 years .
the severities of parkinsonian motor symptoms were as follows : mean total updrs score was 24.7  17.9 ( part 1 , 2.2  2.3 ; part 2 , 7.2  5.8 ; part 3 , 14.8  10.7 ) .
twenty - nine patients had hypertension ( hbp ) , 24 had diabetes mellitus ( dm ) , and 106 were non - smokers . the presence or absence of hypertension and diabetes , and current / past smoking habits did not affect cerebral wmh ( hbp vs. no hbp group : 14.9  8.6 vs. 11.9  9.0 , f = 1.295 , p = 0.258 ; dm vs. no dm group : 15.8  6.7 vs. 11.8  9.3 , f = 2.118 , p = 0.148 ; non - smoker vs. ex - smoker vs. current - smoker 12.9  8.9 vs. 9.8  10.1 vs. 11.7  8.4 , f = 0.259 , p = 0.772 , by ancova controlling for age , ldl - cholesterol and serum homocysteine ) .
higher ldl - cholesterol and serum homocysteine levels also did not correspond to increasing wmh scores ( r = 0.004 , p = 0.965 in ldl - cholesterol ; r = 0.071 , p = 0.494 in homocysteine by spearman s rank correlation ) .
clinical and laboratory profiles of the groups with and without orthostatic hypotension are presented in table 1 .
data from patients with and without orthostatic hypotension were similar for age , gender , duration of illness , severity of parkinsonian motor symptoms , and proportion of patients with hypertension and diabetes . among the 42 patients with oh , 29 were clinically symptomatic ( 69.1% ) .
the wmh scores were higher in the orthostatic hypotension group compared to those in the non - oh group after controlling for age , the presence of hypertension and diabetes , ldl - cholesterol and serum homocysteine . in addition , a trend of more wmh lesions in the supratentorial region of the oh group was also found ( table 2 ) . on correlation analysis ,
orthostatic diastolic blood pressure change was positively correlated with white matter changes ( figure 1a and b ) . on the other hand ,
supine blood pressures were closely correlated with the wmh scores of the whole brain ( figure 1c and d ) . when comparing groups with extremes of wmhs , the severe wmh group had higher supine systolic bp and larger fall in bp during orthostasis ( figure 2 ) .
epidemiological investigations in healthy populations have shown that wmh predict the risk of cerebrovascular disease , cognitive dysfunction , and mortality.1 advanced age , arterial hypertension , diabetes , and elevated serum homocysteine levels are risk factors for increasing wmh.24,14,15 however , these associations remain unclear in patients with pd.5,6 in this study , the presence or absence of cerebrovascular risk factors was not associated with wmh in patients with pd .
this finding is consistent with previous studies and suggests that other nonvascular factors contribute to the vascular brain damage in patients with pd .
orthostatic hypotension is common in patients with pd and appears more frequently as disease progresses and influence subjective symptoms , quality of life , and disease treatment.16 recent investigations in patients with multiple system atrophy indicate that supine systolic blood pressure and/or blood pressure changes during orthostasis are significantly and independently correlated with wmh.17,18 however , a similar phenomenon was not confirmed in patients with pd.18 prior studies were not performed in patients with early stage and the enrolled patients were not free from drug effects . in this study , we enrolled only newly diagnosed patients with early stage pd who had not taken any dopaminergic medication .
because dopaminergic drugs can aggravate orthostatic hypotension , this result suggests that this association was independent from anti - parkinsonian treatment .
we found that the orthostatic hypotension group had more severe wmh changes and that the differences in wmh were more significant in the deep white matter region .
although the hemodynamic consequences during orthostasis have not been clarified , it was reported that systemic blood flow is inversely associated with wmhs in the elderly.19 because orthostatic hypotension leads to recurrent blood pressure drops , this could result in cerebral hypoperfusion and impact neurocirculatory failures , perhaps via cerebral small - vessel disease.20 supine blood pressures can also contribute myriad episodes of cerebral hypo- and hyper - perfusion to white matter injuries .
in addition to neurocirculatory insufficiency , degenerative processes associated with aging and disease itself may contribute to the generation of wmh in pd . in summary ,
our results indicate that hemodynamic changes associated with orthostatic hypotension may be associated with white matter changes in patients with pd .
 background and purpose : several reports on the elderly population have suggested that orthostatic hypotension is associated with white matter hyperintensities ( wmh ) ; however , little information is available on patients with parkinson s disease ( pd).methods : we analyzed the association blood pressure profiles during tilt table testing with wmh scores in 117 patients with pd . 
 wmh were rated using the semiquantitative visual rating system proposed by scheltens et al.results:the presence of orthostatic hypotension was associated with increasing tendency of wmh score and the blood pressure changes during tilting and supine blood pressure were positively correlated with increasing wmh score.conclusions:this finding indicates that hemodynamic changes associated with orthostatic hypotension may be associated with white matter changes in patients with pd . 
Methods
Patients
Tilt table testing
MRI and WMH rating
Data analysis
Results
Discussion