calcium phosphate ceramics have seen extensive clinical application as synthetic bone fillers and graft extenders [ 13 ] .
the biocompatibility as well as osteoconductive and osteoinductive properties of these ceramics have been well documented [ 412 ] .
bone tissue engineering research has capitalized on these qualities , making porous calcium phosphate ceramics a popular choice of scaffold [ 1316 ] .
porous ceramics for medical applications have been manufactured for decades using a variety of traditional methods .
conversion of natural structures , such as coral [ 17 , 18 ] , and trabecular bone [ 19 , 20 ] yield porous ceramics with organic architectures that appear very similar to that of the bone that is being replaced .
synthetic manufacturing methods such as foaming [ 2123 ] , dual - phase mixing   and the slip - casting of polymer foams and particles [ 2527 ] , may also be used to produce porous ceramics
. however , conversion and synthetic techniques result in highly complex macroporous structures that are difficult to define quantitatively . despite the complex nature of the porous ceramics produced by conventional means ,
quite some information is available regarding the influence the porous structure has on osteoconduction [ 2835 ] , bmp induced osteogenesis [ 26 , 3638 ] , and osteoinduction [ 11 , 39 ] .
rapid prototyping ( rp ) , also termed free form fabrication , refers to a variety of technologies capable of producing three - dimensional ( 3d ) physical constructs directly from 3d computer aided models . in recent years
, rapid prototyping has been proposed for the production of both scaffolds with controlled porous architectures [ 4042 ] as well as porous implants with patient specific geometries [ 4345 ] .
several rapid prototyping techniques have been developed to produce ceramic scaffolds for bone tissue engineering research [ 4649 ] .
the aim of the current study was to produce porous ceramic scaffolds from different calcium phosphate materials with sufficiently similar macroporous architectures as to be able to reasonably eliminate the macroporous architecture as a confounding variable in future tissue engineering studies .
the scaffolds were produced by casting four different calcium phosphate materials into identical molds produced using a rapid prototyping technique .
the resulting macroporous structures as well as the chemistry before and after manufacture were evaluated .
briefly , the scaffolds specifications called for an interconnecting network of 400  m square cross - section channels oriented along the orthogonal axes and separated from each other and the exterior by 400  m .
six , four , and three channels were incorporated in the x , y , and z axis directions , resulting in overall design dimensions of 5.2    3.6    2.8  mm , respectively .
1 . molds , with cavities for the production of six scaffolds each , were designed using the rhinoceros computer aided design software ( robert mcneel & associates , usa ) .
the mold model was scaled to account for shrinkage of approximately 20% during thermal processing demonstrated previously by our hydroxyapatite ceramics .
this resulted in pre - thermal processing scaffold dimensions of 6.5    4.5    3.5  mm in the x , y , and z axis directions .
multiple copies of the mold were produced using a modelmaker ii rapid prototyping system ( solidscape inc .
1schematic of the designed scaffolds including the three orthogonal planes used to define the scaffold surfaces schematic of the designed scaffolds including the three orthogonal planes used to define the scaffold surfaces ceramic scaffolds were manufactured to achieve four conditions through combinations of calcium phosphate ceramic compositions and sintering temperatures as outlined in table  1 .
hydroxyapatite powder ( ha , merck , germany ) , beta - tricalcium phosphate powder ( tcp , merck , germany ) and biphasic calcium phosphate powder ( bcp , wt% 85/15 ha / tcp , isotis sa ) were obtained commercially . the ha and
tcp raw powders were calcined by heating from ambient to 900c at a rate of 100c / hour and then cooled naturally with no dwell period .
aqueous slurries of ha , tcp , and bcp powders were prepared as previously described for the production of cast plates . in brief
, the slurry components detailed below were slowly admixed until a homogenous blend was achieved .
the ha and tcp slurries consisted of 67.1 wt% calcined ha powder , 28.6 wt% demineralized water , 2.6 wt% ammonia solution ( 25% , merck ) , 1.5 wt% deflocculant ( dolapix , aschimmer & schwarz gmbh , germany ) .
once a homogeneous blend was obtained , a cmc binder was added ( 0.15 wt% , pomosin bv , the netherlands ) to the ha slurry and the slurry further mixed until homogeneous .
the bcp slurry consisted of 56.4 wt% ceramic powder , 37.6 wt% demineralized water , 3.9 wt% ammonia solution , and 2.1 wt% deflocculant .
all slurries were stored in covered beakers until there use within the same day.table  1scaffold dimensions , shrinkage , volume , weight , and apparent porosity of scaffolds compared to the solids in slurry and sintering temperature during manufacturingmaterialsolids in slurry ( wt%)sintering temp .
( c)exterior dimensions ( mm    sd)srinkage ( from  as molded , % ) volume ( mm    sd)weight ( mg    sd)apparent porosity ( %    sd)ha h67.11250x : 5.05    0.05x : 22.3547.92    0.8776.16    2.8949.65    1.76y :
3.48    0.03y : 22.68z : 2.73    0.03 ha l67.11150x : 6.14    0.05x : 5.4884.63    3.1773.82    5.4072.39    1.27y : 4.23   
0.13y : 6.04z : 3.26    0.11bcp56.41150x : 5.45    0.05x : 16.2158.96    1.3848.86    2.3373.72    1.03y : 3.73    0.06y : 7.86z : 2.90    0.06tcp67.11150x : 6.05    0.04x : 6.8880.53    3.0569.49    4.5472.54    1.10y : 4.15    0.13y : 7.86z : 3.21    0.09 scaffold dimensions , shrinkage , volume , weight , and apparent porosity of scaffolds compared to the solids in slurry and sintering temperature during manufacturing the molds were filled using a simple vacuum device .
france ) were divided in half and the filter paper removed to expose the perforated interior surface .
the open face of a mold was carefully placed against the interior surface of a filter half and secured by circumferentially wrapping with wax laboratory film .
the mold / filter constructs were attached to 50  ml syringes and flushed with demineralized water . during casting ,
the beakers containing slurry were placed on a porex vibrating table ( renfert , germany ) .
this assisted in mold filling by imparting shear energy and thus lowering the viscosity of the pseudoplastic ( shear thinning ) slurries .
the molds were filled by submerging the open face of each mold in slurry and then drawing vacuum pressure using the syringe .
the molds were then placed on a sheet wax laboratory film and the syringes and filter halves removed .
the molds were allowed to air dry overnight at room temperature and were then further dried for 24  h at 50c in air .
excess slurry from each ceramic composition was processed identically to the molded ceramics to serve as controls when examining material chemistry and to further examine the previously observed influence of the rapid prototyping wax on the material composition .
debinding and sintering of the ceramics were performed in two steps in a high temperature furnace ( nabertherm 1400 , germany ) .
debinding of all ceramics was performed by heating at a rate of 0.5c / minute to 400c and then cooling naturally with no dwell period .
the ceramics were then sintered using a 600  min heating phase with a 480  min dwell period at the final sintering temperature followed by natural cooling .
one set of ha scaffold was sintered at 1250c , designate ha h , while a sintering temperature of 1150c was used for a second set of ha scaffolds , designate ha l , as well as all of the tcp and bcp scaffolds .
excess ceramic , occasionally present on the scaffold faces corresponding to the open sides of the molds , was removed using a rotary polisher ( labopol-5 , struers , denmark ) with 1200 grit waterproof silicon carbide paper ( struers ) .
the ceramics were cleaned by ultrasound for 15  min each in acetone , 100% ethanol and deionized water , and then dried in air at 50c .
the exterior scaffold dimensions were measured using a digital caliper ( cd-15c , mitutoyo ltd . , uk ) and used to calculate the shrinkage resulting from the combined debinding and sintering processes . scanning electron microscopy ( sem , xl 30 esem - feg , philips , the netherlands ) was used to examine the macro - architecture and surface micro - structure of the scaffolds .
the dimensions of the macroporosity were measured in each of the orthogonal planes ( fig .  1 ) .
the apparent porosity of the scaffolds was determined by comparing the apparent density of each scaffold ( dry weight / measured volume ) and the theoretical density of ha ( 3.156  g / cm ) , tcp ( 3.14  g / cm3 ) , and bcp ( 85% ha ,
the chemistry of raw ceramic powder , calcined ceramic powder , non - molded sintered ceramic and molded scaffolds were evaluated by x - ray diffraction ( xrd , miniflex , rigaku , japan ) .
finally , the potential contamination of the ceramics by residues from the wax mold material was investigated by performing energy - dispersive x - ray spectroscopy ( edx , xl 30 esem - feg , philips , the netherlands ) on the surface of cast and non - cast ( not exposed to wax mold material ) ceramics specimens .
briefly , the scaffolds specifications called for an interconnecting network of 400  m square cross - section channels oriented along the orthogonal axes and separated from each other and the exterior by 400  m .
six , four , and three channels were incorporated in the x , y , and z axis directions , resulting in overall design dimensions of 5.2    3.6    2.8  mm , respectively .
1 . molds , with cavities for the production of six scaffolds each , were designed using the rhinoceros computer aided design software ( robert mcneel & associates , usa ) .
the mold model was scaled to account for shrinkage of approximately 20% during thermal processing demonstrated previously by our hydroxyapatite ceramics .
this resulted in pre - thermal processing scaffold dimensions of 6.5    4.5    3.5  mm in the x , y , and z axis directions .
multiple copies of the mold were produced using a modelmaker ii rapid prototyping system ( solidscape inc .
1schematic of the designed scaffolds including the three orthogonal planes used to define the scaffold surfaces schematic of the designed scaffolds including the three orthogonal planes used to define the scaffold surfaces
ceramic scaffolds were manufactured to achieve four conditions through combinations of calcium phosphate ceramic compositions and sintering temperatures as outlined in table  1 .
hydroxyapatite powder ( ha , merck , germany ) , beta - tricalcium phosphate powder ( tcp , merck , germany ) and biphasic calcium phosphate powder ( bcp , wt% 85/15 ha / tcp , isotis sa ) were obtained commercially . the ha and
tcp raw powders were calcined by heating from ambient to 900c at a rate of 100c / hour and then cooled naturally with no dwell period .
aqueous slurries of ha , tcp , and bcp powders were prepared as previously described for the production of cast plates . in brief
, the slurry components detailed below were slowly admixed until a homogenous blend was achieved .
the ha and tcp slurries consisted of 67.1 wt% calcined ha powder , 28.6 wt% demineralized water , 2.6 wt% ammonia solution ( 25% , merck ) , 1.5 wt% deflocculant ( dolapix , aschimmer & schwarz gmbh , germany ) .
once a homogeneous blend was obtained , a cmc binder was added ( 0.15 wt% , pomosin bv , the netherlands ) to the ha slurry and the slurry further mixed until homogeneous .
the bcp slurry consisted of 56.4 wt% ceramic powder , 37.6 wt% demineralized water , 3.9 wt% ammonia solution , and 2.1 wt% deflocculant .
all slurries were stored in covered beakers until there use within the same day.table  1scaffold dimensions , shrinkage , volume , weight , and apparent porosity of scaffolds compared to the solids in slurry and sintering temperature during manufacturingmaterialsolids in slurry ( wt%)sintering temp .
( c)exterior dimensions ( mm    sd)srinkage ( from  as molded , % ) volume ( mm    sd)weight ( mg    sd)apparent porosity ( %    sd)ha h67.11250x : 5.05    0.05x : 22.3547.92    0.8776.16    2.8949.65    1.76y : 3.48    0.03y : 22.68z : 2.73    0.03 ha l67.11150x : 6.14    0.05x : 5.4884.63    3.1773.82    5.4072.39    1.27y : 4.23    0.13y : 6.04z : 3.26    0.11bcp56.41150x : 5.45    0.05x : 16.2158.96    1.3848.86    2.3373.72    1.03y : 3.73    0.06y : 7.86z : 2.90    0.06tcp67.11150x : 6.05    0.04x : 6.8880.53   
3.0569.49    4.5472.54    1.10y : 4.15    0.13y : 7.86z : 3.21    0.09 scaffold dimensions , shrinkage , volume , weight , and apparent porosity of scaffolds compared to the solids in slurry and sintering temperature during manufacturing
france ) were divided in half and the filter paper removed to expose the perforated interior surface .
the open face of a mold was carefully placed against the interior surface of a filter half and secured by circumferentially wrapping with wax laboratory film .
the mold / filter constructs were attached to 50  ml syringes and flushed with demineralized water . during casting ,
the beakers containing slurry were placed on a porex vibrating table ( renfert , germany ) .
this assisted in mold filling by imparting shear energy and thus lowering the viscosity of the pseudoplastic ( shear thinning ) slurries .
the molds were filled by submerging the open face of each mold in slurry and then drawing vacuum pressure using the syringe .
the molds were then placed on a sheet wax laboratory film and the syringes and filter halves removed .
the molds were allowed to air dry overnight at room temperature and were then further dried for 24  h at 50c in air .
excess slurry from each ceramic composition was processed identically to the molded ceramics to serve as controls when examining material chemistry and to further examine the previously observed influence of the rapid prototyping wax on the material composition .
debinding and sintering of the ceramics were performed in two steps in a high temperature furnace ( nabertherm 1400 , germany ) .
debinding of all ceramics was performed by heating at a rate of 0.5c / minute to 400c and then cooling naturally with no dwell period .
the ceramics were then sintered using a 600  min heating phase with a 480  min dwell period at the final sintering temperature followed by natural cooling .
one set of ha scaffold was sintered at 1250c , designate ha h , while a sintering temperature of 1150c was used for a second set of ha scaffolds , designate ha l , as well as all of the tcp and bcp scaffolds .
excess ceramic , occasionally present on the scaffold faces corresponding to the open sides of the molds , was removed using a rotary polisher ( labopol-5 , struers , denmark ) with 1200 grit waterproof silicon carbide paper ( struers ) .
the ceramics were cleaned by ultrasound for 15  min each in acetone , 100% ethanol and deionized water , and then dried in air at 50c .
the exterior scaffold dimensions were measured using a digital caliper ( cd-15c , mitutoyo ltd . , uk ) and used to calculate the shrinkage resulting from the combined debinding and sintering processes . scanning electron microscopy
( sem , xl 30 esem - feg , philips , the netherlands ) was used to examine the macro - architecture and surface micro - structure of the scaffolds .
the dimensions of the macroporosity were measured in each of the orthogonal planes ( fig .  1 ) .
the apparent porosity of the scaffolds was determined by comparing the apparent density of each scaffold ( dry weight / measured volume ) and the theoretical density of ha ( 3.156  g / cm ) , tcp ( 3.14  g / cm3 ) , and bcp ( 85% ha , 15% tcp ) .
the chemistry of raw ceramic powder , calcined ceramic powder , non - molded sintered ceramic and molded scaffolds were evaluated by x - ray diffraction ( xrd , miniflex , rigaku , japan ) .
finally , the potential contamination of the ceramics by residues from the wax mold material was investigated by performing energy - dispersive x - ray spectroscopy ( edx , xl 30 esem - feg , philips , the netherlands ) on the surface of cast and non - cast ( not exposed to wax mold material ) ceramics specimens .
the manufacturing process resulted in scaffolds with remarkably similar structural appearances ( fig .  2 ) .
scaffold dimensions , shrinkage , volume , weight and apparent porosity values are summarized in table  1 .
the bcp scaffolds also demonstrated considerable shrinkage but maintained a high apparent porosity similar to the low sintering temperature ha and tcp scaffolds . the low sintering temperature ha and tcp scaffolds exhibited the lowest shrinkage .
shrinkage in the z - direction and volumetric shrinkage were not calculated since the respective surfaces were manually polished to remove excess ceramic and therefore do not represent the  as cast properties . in order to evaluate whether the various treatments influenced the ratio of macroporosity to total porosity , computer models of the porous scaffolds were created using the measured exterior and macropore dimensions in tables  1 and 2 , respectively .
table  3 shows the volumes approximated by the computer models for the various treatments and compares the resulting macroporosity to the measured apparent porosity.fig . 
note the similarity of the scaffold structures and the differences in the scaffold colorstable  2pore dimensions by orthogonal plane ( fig . 
1)materialpore dimensions ( m    sd)x  y planex  z planey  z planeha hx : 286    15x : 353    28y : 394    24y : 280    16z : 339    17z : 376    30 ha lx : 414    44x : 470    37y : 484    29y : 416    34z : 496    21z : 486   
27bcpx : 366    24x : 444    47y : 432    37y : 377    18z : 433    20z : 414    42tcpx : 405    43x : 460    36y : 474    29y : 408    33z : 486    21z : 476    26table  3scaffold volumes and macroporosity calculated form computer models compared to the measured total apparent porosity and other porosity ( difference between macro and apparent porosity)materialtotal volume ( mm)material volume ( mm)pole volume ( mm)macro - porosity ( % ) apparent porosity ( % ) other porosity ( % ) ha h47.9831.7816.1933.7549.6515.90 ha l84.6750.1934.4840.7272.3931.67bcp58.9534.8224.1440.9473.7232.78tcp80.6048.0832.5140.3472.5432.20 the four ceramic compositions all in 25 well plates .
note the similarity of the scaffold structures and the differences in the scaffold colors pore dimensions by orthogonal plane ( fig . 
1 ) scaffold volumes and macroporosity calculated form computer models compared to the measured total apparent porosity and other porosity ( difference between macro and apparent porosity ) sem images of the resulting scaffolds are shown in fig .  3 .
these scaffolds are discussed in the following text using the axes and orthogonal planes depicted in fig .  1 . a distinctive texture of parallel ridges and valleys was observed in sem micrographs on all vertical scaffold surfaces , i.e. , surfaces parallel to the x  z and y  z planes .
this texture is an impression of the rapid prototyped mold and a consequence of the layer - by - layer manufacturing of the mold .
the cross - sectional geometry of the channels was dependant upon the orientation of the channel . channels running in the x- and y - directions were square in cross section with textured vertical surfaces and smooth horizontal surfaces .
rows top to bottom   ha h , ha l , bcp , and tcp .
first column perspective view of scaffolds at 50 magnification ( bar 50  m ) .
fourth column scaffold surfaces at 1000 magnification ( bar 20  m ) sem micrographs of the four scaffold materials .
rows top to bottom   ha h , ha l , bcp , and tcp .
first column perspective view of scaffolds at 50 magnification ( bar 50  m ) .
second column scaffold structures at 100 magnification ( bar 200  m ) . note the regular surface texture on the scaffolds .
fourth column scaffold surfaces at 1000 magnification ( bar 20  m ) the surface microporosity of the scaffolds , as observed by sem , varied with material composition and sintering temperature ( fig .  3 ) .
the bcp scaffolds exhibited a spectrum of surface microporosity features from approximately 1 to 10  m in size .
the surface features of the low sintering temperature ha scaffolds were similar to the bcp with perhaps somewhat less and smaller surface microporosity , approximately 0.55  m .
the tcp material , in contrast to the other materials sintered at low temperatures , appeared very similar to the high sintering temperature ha with very little surface microporosity .
the xrd patterns for the different ceramic chemistries , shown in fig .  4 , were generally as expected .
figure  4a shows the patterns for the ha ceramic raw powder , calcined powder , non - molded material sintered at 1150c , scaffolds sintered at 1150c , non - molded material sintered at 1250c , and scaffolds sintered at 1150c .
several peaks associated with tcp formation were observed in the patterns for the cast ha scaffold materials at both the 1150 and 1250c sintering temperatures ( vertical lines in fig . 
the bcp ceramics also demonstrated these tcp peaks in the cast scaffolds ( fig .  4b , vertical lines ) .
the tcp ceramics exhibited changes , relative to the raw powder , that were consistent with the calcination and sintering process temperatures ( fig . 
the xrd patterns for the four scaffold conditions are shown in fig .  5 for clarity .
edx of the surfaces of both cast and non - cast ceramic specimens showed identical spectra consistent with the calcium phosphate materials .
4xrd patterns of a ha h ( 1250 ) and ha l ( 1150 ) , b bcp , and c tcp . shown
are xrd patterns of the raw powder , calcined powder and molded ceramics ( scaffolds ) .
xrd patterns of non - molded ceramics are also shown in ( a ) for the ha h ( 1250 ) and ha l ( 1150 ) materials .
vertical dotted lines indicate additional peaks associated with beta - tcp formation that are only present in the molded specimens ( scaffolds)fig . 
5xrd patterns of the four scaffold materials . vertical dotted lines indicate beta - tcp peaks which form in the ha and bcp material as a result of the molding process xrd patterns of a ha h ( 1250 ) and ha l ( 1150 ) , b bcp , and c tcp .
shown are xrd patterns of the raw powder , calcined powder and molded ceramics ( scaffolds ) .
xrd patterns of non - molded ceramics are also shown in ( a ) for the ha h ( 1250 ) and ha l ( 1150 ) materials .
vertical dotted lines indicate additional peaks associated with beta - tcp formation that are only present in the molded specimens ( scaffolds ) xrd patterns of the four scaffold materials .
vertical dotted lines indicate beta - tcp peaks which form in the ha and bcp material as a result of the molding process
the present study demonstrates the application of computer aided design and rapid prototyping technologies for the production of ceramic scaffolds from different chemistries but with defined , virtually identical , macro - architectures .
other than producing macroporosities with pore dimension in the range suggested in the literature for osteoconduction , i.e. , between 50 and 500  mm [ 4 , 28 , 30 , 31 , 34 ] , we did not attempt to produce optimal or ideal porous structures .
the purpose of this study was to manufacture porous scaffolds in which the macroporous architecture was designed and sufficiently similar to be able to reasonable exclude the macroporous architecture as a confounding variable in future research studies .
the material chemistries and thermal processing methods employed in this study were chosen to provide continuity with materials used in past and ongoing research [ 4954 ] .
although the visual appearance of the scaffolds was similar with regard to structure , there were differences in shrinkage and therefore the macroporous dimensions .
as expected from our previous work , a sintering temperature of 1250c resulted in a shrinkage of just over 22% for the ha material compared to approximately 6% shrinkage for ha and tcp sintered at 1150c .
the relatively large shrinkage of 1617% for the bcp scaffolds , also sintered at 1150c , can almost completely be accounted for by the lower solids loading of the bcp slurry ( 56.4 wt% ) compared to the ha and tct slurries ( 67.1 wt% ) .
the lower solids loading was necessary to achieve appropriate rheological properties for the casting of scaffolds from slurries of the non - calcined bcp powder .
interestingly , the apparent porosity of bcp scaffolds was very similar to the tcp and low sintering temperature ha scaffolds despite the much higher shrinkage ( table  2 ) . comparing the porosity resulting from the measured macroporous structure to the total apparent porosity , table  3 , reveals that the a much greater proportion of the apparent porosity of the high sintering temperature ha is likely due to the macroporosity compared to the lower sintering temperature materials .
the bcp , tcp and low sintering temperature ha all had similar proportions of macroporosity despite the much higher shrinkage of the bcp material .
the texture exhibited on the vertical surfaces of the scaffold ( fig .  1 ) , as well as the rounded nature of the macropores in the z - direction , are a consequence of the rapid prototyping technique used to manufacture the molds .
this technique jets molten droplets of wax material , which flatten and spread when they strike the surface , to build each layer of the molds .
as molds are built up vertically layer by layer , this results in a texture on the vertical surfaces ( x  z and y  z planes ) which is subsequently cast into the ceramic .
the rounded corners of channels running in the z - direction ( cross - sections parallel to the x  y plane ) result from the coalescing or pooling of adjacent droplets prior to solidification , resulting in rounding of both inside and outside corners within the printed layers .
these rounded mold corners are then cast into the resulting ceramic scaffolds and observed in channels running parallel to the z - direction .
ha and bcp scaffolds indicated that a tcp phase had been introduced . since the xrd patterns of the non - molded specimens did not show the tcp phase and the molded and non - molded materials were treated identically with the exception of the molding process , it is likely that the presence of tcp phase after molding results from the exposure of the ha and bcp materials to the wax mold material itself , despite elemental analysis of cast and non - cast specimens demonstrated there was no direct contamination of the ceramics by the mold material .
the mechanism for this is not clear but is consistent with our previous findings for ha materials . in conclusion
, we have demonstrated a rapid prototyping method for fabricating ceramic scaffolds with virtually identical , 3-dimensional , macroporous architectures from different calcium phosphate ceramics .
scaffolds produced by this method will not only enhance research aimed at optimizing macroporous architectures and material compositions but will improve many other aspects of tissue engineering research by eliminate differences in macroporous structure as a confounding variable .
 calcium phosphate ceramics , commonly applied as bone graft substitutes , are a natural choice of scaffolding material for bone tissue engineering . 
 evidence shows that the chemical composition , macroporosity and microporosity of these ceramics influences their behavior as bone graft substitutes and bone tissue engineering scaffolds but little has been done to optimize these parameters . 
 one method of optimization is to place focus on a particular parameter by normalizing the influence , as much as possible , of confounding parameters . 
 this is difficult to accomplish with traditional fabrication techniques . in this study 
 we describe a design based rapid prototyping method of manufacturing scaffolds with virtually identical macroporous architectures from different calcium phosphate ceramic compositions . 
 beta - tricalcium phosphate , hydroxyapatite ( at two sintering temperatures ) and biphasic calcium phosphate scaffolds were manufactured . 
 the macro- and micro - architectures of the scaffolds were characterized as well as the influence of the manufacturing method on the chemistries of the calcium phosphate compositions . 
 the structural characteristics of the resulting scaffolds were remarkably similar . 
 the manufacturing process had little influence on the composition of the materials except for the consistent but small addition of , or increase in , a beta - tricalcium phosphate phase . among other applications , scaffolds produced by the method described 
 provide a means of examining the influence of different calcium phosphate compositions while confidently excluding the influence of the macroporous structure of the scaffolds . 
Introduction
Materials and methods
Scaffold design and mold fabrication
Ceramic slurries
Scaffold fabrication
Scaffold characterization
Results
Discussion and conclusions