prevention of dental caries is one of the main strategies in contemporary pediatric dental practice .
the dental plaque is a structure of vital significance as a contributing factor to at least the initiation of the carious lesion .
the major strains of streptococci present in plaque are : streptococcus mutans , streptococcus sanguis , streptococcus mitior , streptococcus milleri and streptococcus salivarius . of these ,
although mechanical plaque control by tooth brushing is the most dependable and commonly practiced oral hygiene measure , numerous anti - plaque agents have been tried for improving oral health .
these agents are capable of preventing bacterial adhesion , colonization and metabolism , and thus affect the bacterial growth .
the efficacy of these antimicrobial agents depends on factors like vehicle used , concentration of active agents , substantivity of the agent and duration of the treatment .
an ideal anti - plaque agent for regular use in children should not interfere with biologic processes occurring in the mouth , be harmless to oral mucosa , should have low toxicity if accidentally swallowed , and should be both sugar and alcohol free .
fluoride is one of the most important and effective components of preventive dental programs in children .
sodium fluoride mouth rinses are effective in reducing caries and inhibit carbohydrate utilization of oral microorganisms by blocking enzymes involved in the bacterial gylcolytic pathway .
xylitol is a naturally occurring non - cariogenic sugar substitute that can not be metabolized by oral bacteria .
xylitol reduces the amount of adherent extracellular polysaccharides , lipoteichoic acids , resulting in the formation of loosely attached bio - films to the tooth surfaces .
most in vivo studies have focused on the application of xylitol chewing gums , inextricably combining physicochemical with mechanical effects .
although it is incorporated in certain toothpastes , data available on mouth rinses containing triclosan are limited .
thus , the aim of this study was to investigate the efficacy of a newly introduced xylitol , sodium fluoride and triclosan containing mouth rinse in reducing the levels of plaque s. mutans and to compare it with that of a 0.12% chlorhexidine mouth rinse .
eighty - five normal children aged between 7 and 13 years and residing in an orphanage in bangalore city were screened as part of a routine dental examination .
these children had the same dietary pattern and followed similar oral hygiene practices . prior to the study ,
ethical clearance was obtained from the ethical committee of the institution . to be included in the study ,
each child had to have a dental caries score ( deft / dmft score ) equal to or greater than 3 . 

medically compromised childrenchildren with a history of taking antibiotics 3 months prior to and during the study periodchildren undergoing orthodontic treatment or with an intraoral prosthesischildren who could not brush their teeth or rinse on their ownpresence of any intraoral soft tissue pathology 
  medically compromised children children with a history of taking antibiotics 3 months prior to and during the study period children undergoing orthodontic treatment or with an intraoral prosthesis children who could not brush their teeth or rinse on their own presence of any intraoral soft tissue pathology thirty children formed the study group .
the authorities of the orphanage were instructed not to take these children for any dental treatment during the study period . at the onset of the study , each child was given a new soft toothbrush and non - fluoridated toothpaste .
demonstration of tooth brushing using scrub technique was given to all children by the investigator .
they were instructed to brush before breakfast in the morning and after meals in the night . prior to commencement of the oral rinse regime ,
autoclaved wooden toothpicks were used to take plaque samples from the buccal surface of a noncarious permanent mandibular first molar or a primary mandibular second molar .
the samples were taken to the laboratory within an hour , serial dilutions prepared and vortexed .
one milliliter of the dilution was inoculated onto mitis salivarius agar and incubated at 37c for 48 hours .
colony forming units ( cfus ) of s. mutans were counted at baseline with the help of a digital colony counter . in this single - blind trial , the children were randomly divided into two groups of 15 children each .
group i ( study group ) was given a mouth rinse containing xylitol ( 5% ) , sodium fluoride ( 0.05% ) and triclosan ( 0.03% ) ( kidodent mouthwash , indoco remedies ltd , mumbai , india ) , and group ii ( control group ) was given a chlorhexidine ( 0.12% ) mouth rinse ( nitra hex , micro labs limited , bangalore , india ) .
the children were asked to gather in a large room , where each child was given 10 ml of the mouth rinse and asked to rinse for 1 minute .
mouth rinsing was carried out twice daily , half an hour after breakfast and half an hour following dinner , for a period of 21 days under the supervision of the investigator . on the 22 day , prior to breakfast ,
plaque samples were collected , processed and s. mutans counts were assessed and compared with baseline values .
data obtained were subjected to statistical analysis using student 's t - test . in order to ascertain preference of mouth rinse ,
all the children were asked to taste both mouth rinses at the end of the study .

 medically compromised childrenchildren with a history of taking antibiotics 3 months prior to and during the study periodchildren undergoing orthodontic treatment or with an intraoral prosthesischildren who could not brush their teeth or rinse on their ownpresence of any intraoral soft tissue pathology 
  medically compromised children children with a history of taking antibiotics 3 months prior to and during the study period children undergoing orthodontic treatment or with an intraoral prosthesis children who could not brush their teeth or rinse on their own presence of any intraoral soft tissue pathology thirty children formed the study group .
the authorities of the orphanage were instructed not to take these children for any dental treatment during the study period . at the onset of the study , each child was given a new soft toothbrush and non - fluoridated toothpaste .
demonstration of tooth brushing using scrub technique was given to all children by the investigator .
they were instructed to brush before breakfast in the morning and after meals in the night .
prior to commencement of the oral rinse regime , autoclaved wooden toothpicks were used to take plaque samples from the buccal surface of a noncarious permanent mandibular first molar or a primary mandibular second molar .
the samples were taken to the laboratory within an hour , serial dilutions prepared and vortexed .
one milliliter of the dilution was inoculated onto mitis salivarius agar and incubated at 37c for 48 hours .
colony forming units ( cfus ) of s. mutans were counted at baseline with the help of a digital colony counter . in this single - blind trial , the children were randomly divided into two groups of 15 children each .
group i ( study group ) was given a mouth rinse containing xylitol ( 5% ) , sodium fluoride ( 0.05% ) and triclosan ( 0.03% ) ( kidodent mouthwash , indoco remedies ltd , mumbai , india ) , and group ii ( control group ) was given a chlorhexidine ( 0.12% ) mouth rinse ( nitra hex , micro labs limited , bangalore , india ) .
the children were asked to gather in a large room , where each child was given 10 ml of the mouth rinse and asked to rinse for 1 minute .
mouth rinsing was carried out twice daily , half an hour after breakfast and half an hour following dinner , for a period of 21 days under the supervision of the investigator .
on the 22 day , prior to breakfast , plaque samples were collected , processed and s. mutans counts were assessed and compared with baseline values .
data obtained were subjected to statistical analysis using student 's t - test . in order to ascertain preference of mouth rinse ,
all the children were asked to taste both mouth rinses at the end of the study .
in both groups , there was a significant reduction in the mean s. mutans count at the end of 21 days ( p < 0.001 ) .
of the 30 children , 83.33% preferred the taste of xylitol , sodium fluoride and triclosan containing mouth rinse , while 16.67% preferred the taste of 0.12% chlorhexidine mouth rinse [ figure 1 ] .
it is important for these products to be effective and safe for regular use in children .
mouth rinses are recommended only for those who have the ability to swish and expectorate without swallowing .
most mouth rinses contain alcohol as one of their ingredients and they are also available  over the counter . 
the major side effects of alcohol containing mouth rinses include the presence of oral pain , burning sensation , difficulty of use in patients with oral sensitivity and the risk of accidental alcohol ingestion in children.[1012 ] the extrahepatic metabolism of alcohol has been demonstrated in oral tissue . in the human mouth , aldehyde dehydrogenase ( aldh ) , an enzyme that converts acetaldehyde into a nontoxic acetate compound , occurs less frequently than alcohol dehydrogenase ( adh ) .
this imbalance allows for the accumulation in oral tissues of a toxic , reactive and irritating acetaldehyde .
due to a point mutation , aldehyde dehydrogenase 2 ( aldh2 ) isoenzyme is deficient in 3050% of asians .
these individuals have a genetic inability to remove acetaldehyde and consequently have very high salivary acetaldehyde levels after moderate dose of alcohol .
hence , in this study , it was primarily necessary to select two mouth rinses that were alcohol free . in recent times , various polyalcohols , particularly xylitol , have been incorporated in a number of different products for children , including mouth rinses .
the nonspecific effect of xylitol is due its non - fermentability , and thus does not encourage bacterial growth . the selective effect on mutans streptococci results in the development of mutant resistant strains which may be less virulent in the oral environment .
 metabolic cycle in which xylitol is taken into the cell , phosphorylated to xylitol-5-phosphate , split into sugar phosphate phosphatases and the resulting xylitol is expelled from the cell .
the ability of plaque to produce acids by the metabolism of sugars is reduced by xylitol .
xylitol appears to have a unique effect in reducing adhesion and it is expected that other polyols might not show this clinical effect .
the concentrations of ammonia and basic amino acids increase when plaque is exposed to xylitol , resulting in neutralization of plaque acids .
the presence of fluoride in this mouth rinse could have also contributed to the reduction of s. mutans in plaque .
the effects of fluoride on streptococcal cells are partly ascribed to the inhibition of enolase , one of the series of gylcolytic enzymes .
this inhibition decreases the intracellular level of phosphoenolpyruvate ( pep ) , and thus decreases bacterial sugar uptake via pep - dependent phosphotransferase system ( pep - pts ) .
in addition , fluoride can directly inhibit bacterial proton - translocating atpase that is considered to partly contribute to the proton excertion out of the cells , leading to acidification of intracellular ph .
the dissociation of unionized hydrofluoric acid into h+ and f in the cells also promotes intracellular acidification .
analyses of intracellular gylcolytic intermediates revealed that xylitol inhibited the upper part of the gylcolytic pathway , while fluoride inhibited the lower part .
this suggests that fluoride and xylitol together have synergistic inhibitory effects on the acid production of mutans streptococci and that xylitol has the potential to enhance inhibitory effects of low concentrations of fluoride .
however , fluoride mouth rinses should be reserved for use with children judged to be at moderate or high risk for dental caries .
it is also important to select a fluoridated mouth rinse with a fluoride concentration of 0.05% for regular use .
the other ingredient present is triclosan , a broad - spectrum antimicrobial , having an anti - plaque potential .
triclosan ( 2,4,4-trichloro 2-hydroxydiphenyl ether ) is used to increase the ability of mouthwashes to bind to the oral mucosa , and thus be available for longer periods of time .
jenkins et al . compared the magnitude and duration of salivary bacterial count reductions produced by a single rinse of 0.2% triclosan , 1% sodium lauryl sulfate ( sls ) and 0.2% chlorhexidine mouthwashes .
they found considerable reductions in bacterial counts which remained significant for 3 hours with triclosan and for 7 hours with sls and chlorhexidine .
the use of 0.3% triclosan mouth rinse showed significant reduction in salivary mutans streptococci count .
hence , the significant reduction in plaque s. mutans observed in our study could be attributed to the synergistic effect of all three constituents , viz .
it is an effective anti - plaque agent and is widely available as a mouth rinse .
chlorhexidine is a bis - biguanide which is effective against gram - positive bacteria , gram - negative bacteria and yeast . at relatively high concentration , chlorhexidine is bactericidal ; but at low concentration , it is bacteriostatic .
low concentrations of chlorhexidine allow cytoplasmic constituents to leak out , while a high concentration coagulates them .
it inhibits the membrane atpase and anaerobic process . in a study on 1214-year - old children with high caries risk ,
0.12% chlorhexidine mouth rinse was more efficient in reducing mutans streptococci count in saliva , as compared to other mouth rinses .
however , it gave more negative findings when taste sensation and side effects were compared .
a recent review concluded that due to the current lack of long - term clinical evidence for caries prevention and reported side effects , chlorhexidine rinses should not be recommended for caries prevention .
since we found no significant difference between both the mouth rinses , with regard to their efficacy in reducing s. mutans , the use of a low fluoride  xylitol based mouth rinse appears to be a suitable choice for regular use in children .
also , children preferred the taste of the bubble gum flavored , xylitol - based mouth rinse . since this study was a brief clinical trial on a newly introduced mouth rinse for children , studies of longer duration
further research on individual components and their effect on saliva , teeth and remineralization potential would enable clinicians to select an appropriate mouth rinse for use in children .
significant reduction inplaque levels of s. mutans was observed with the use of xylitol , sodium fluoride and triclosan containing mouth rinse .
 introduction : prevention of dental caries is one of the main strategies in contemporary pediatric dental practice . 
 mouth rinses are widely used as an adjunct to maintain oral hygiene . 
 it is important for these products to be effective and safe for regular use in children.objective:the aim of the study was to investigate the efficacy of a newly introduced xylitol , sodium fluoride and triclosan containing mouth rinse in reducing levels of plaque streptococcus mutans and to compare it with that of a 0.12% chlorhexidine mouth rinse.materials and methods : thirty children were randomly divided into two groups of 15 children each . 
 group i ( study group ) was given a mouth rinse containing xylitol ( 5% ) , sodium fluoride ( 0.05% ) and triclosan ( 0.03% ) and group ii ( control group ) was given a chlorhexidine ( 0.12% ) mouth rinse . 
 both mouth rinses were alcohol free . 
 mouth rinsing was carried out twice daily , half an hour after breakfast and half an hour following dinner , for a period of 21 days under the supervision of the investigator.results:in both groups , there was a significant reduction in the mean s. mutans count at the end of 21 days ( p < 0.001 ) . 
 no significant difference was observed between the two mouth rinses.conclusion:the use of a low fluoride  xylitol based mouth rinse appears to be a suitable choice for regular use in children . 
Introduction
Materials and Methods
Exclusion criteria
Results
Discussion
Conclusion