it is estimated that 6685  % of the world s population depends directly on plants as medicines [ 13 ] . since the existence of human civilization , plants and their by - products have been used by a large proportion of the population living in rural and urban areas for various purposes such as medicine , healthcare , food , clothing , shelter , agriculture , agrochemicals , pharmaceuticals , narcotics , etc .
medicinal plants are defined as plants having one or more organs containing substances that can be used for therapeutic purposes or which are precursors for the synthesis of useful drugs .
thus , medicinal plants represent , for the local population , a possibility of simple and cheap treatment . in addition
, they represent sources of potentially important new pharmaceutical substances since all parts of a plant , from roots to seed heads and flowers , are employed in traditional remedies and can therefore act as sources of lead compounds . more than 80  % of the world s populations , especially in developing countries , depend on traditional systems of medicine for the treatment of a variety of diseases .
this observation could be attributed to two main factors ; inaccessibility of modern drugs and the low purchasing power within the populations living in the rural areas . moreover ,
some of the local remedies work and so the populations have no need for anything different . from antiquity , mankind has been developing a traditional medicinal system , based on the knowledge of medicinal plants throughout the world .
africans , in particular , have used medicinal plants and animal - derived remedies in their struggle for survival and in their quest for religious experiences . the world health organization ( who ) defines traditional medicine as practices , knowledge and belief systems which use minerals , plants and animal based remedies , spiritual therapies and exercises to prevent , treat and
this knowledge became enriched over numerous generations due to experimentation but also through observations of animal behaviour .
most often , knowledge of traditional medicine is only inherited orally , thereby facing the danger of being lost in favor of western medicine ( wm ) .
many africans believe in the manifestation of life forces or spirits in every creation , and that these spirits constitute the heart of all life forms , natural events or non - living things .
this gives herbal medicine a vital role in health care delivery systems especially in remote areas where clinics and hospitals are sparsely located [ 8 , 11 ] . despite the advances in wm , african traditional medicine ( atm )
this could probably be due to the rapidly increasing awareness of the potential and curative abilities of alternative medicines , especially from the use of medicinal plants , as well as the inadequate access to wm and physicians and the high cost for western drugs .
the argument for the local african populations resorting to traditional remedies could also be partly justified by the fact that natural product inspired molecules represented about 80  % of drugs that had been put into the market [ 1 , 13 ] .
previous ethnobotanical studies of medicinal plants confirm the rational use of recipes by different people or groups from different communities .
numerous varieties of medicinal plants growing in africa are widely used against many diseases ranging from endemic tropical diseases like malaria   and leishmaniasis   to complex illnesses such as asthma , psychosis , hepatitis   and even cancer .
searching for new lead compounds to be developed as drugs or as templates for analogue synthesis and the evaluation of traditional medicine and herbal medicinal products , are the two basic reasons for the advancement of work on medicinal plants .
the use of plant preparations can be supported if it is safe and if their biological activity can be scientifically confirmed .
this calls for quality control and standardization . if the activity can not be confirmed , and certainly if there is a risk of toxicity , the use of herbal medicinal products should be discouraged .
the study of plants used traditionally as medicines is therefore an interesting discipline because of the possibility to find new drugs and also because of the strong adhesion of local populations , for the aforementioned reasons [ 1113 , 22 , 23 ] .
this has prompted many research teams to carry out studies on plants used in africa by traditional healers against diseases .
the united nations definition of western africa includes the following 16 countries : benin , burkina faso , cape verde , ivory coast , the gambia , ghana , guinea , guinea - bissau , liberia , mali , mauritania , niger , nigeria , senegal , sierra leone and togo .
these countries occupy an area of over 6,140,000  km and the natural environment in this area consists of subtropical and tropical regions with semi - arid and humid climates . in these communities ,
traditional herbalists operate closer to the people , taking advantage of the biodiversity of plant species in such areas to cure various diseases and ailments .
nigeria is located in west africa on the gulf of guinea and shares borders with cameroon ( 1,690  km ) in the east , chad ( 87  km ) in the northeast , niger ( 1,497  km ) in the north , benin ( 773  km ) in the west and the atlantic ocean in the south ( fig .  1 ) .
the country is divided administratively into the federal capital territory ( abuja ) and 36 states . as a result of the large surface area occupied by nigeria
these include about 20,000 species of insects , almost 1,000 species of birds , 247 species of mammals , 123 species of reptiles , about 1,000 species of fish and about 7,895 species of plants .
consequently atm is a common and acceptable practice in nigeria as in many countries in africa and asia .
many plants from the nigerian flora which provide very useful clues for potential therapeutic compounds have been investigated by mostly university research groups .
some of the plants investigated are commonly used in the treatment of several microbial infections , a range of neglected tropical diseases including malaria , trypanosomiasis , as well as diabetes   and diverse infections .
it is therefore very important to scientifically document the available knowledge for its exploitation towards the enhancement of human health .fig . 
1map showing nigeria and her neighbours   map showing nigeria and her neighbours   several attempts towards the documenting of ethnobotanical and ethnopharmacological knowledge for medicinal plants in nigeria have been carried out .
raphael has provided a useful work on traditional medicine in nigeria , about its current status and the future , while soladoye et al .  
provided a list of 143 plant species belonging to 58 families which were found to be useful for the treatment of haemorrhoids in south western nigeria .
.   also carried out an ethnobotanical survey around ogun state , south western nigeria , in which 38 plant species belonging to 24 families were identified to be used in herbal anti - malarial recipes , while ajibesin et al .   identified a total of 114 medicinal plant species representing 102 genera and 54 families employed in the traditional medical practice of the people of akwa ibom state , nigeria .
.   carried out a similar survey of wound - healing plants , while sonibare and gbile , listed plants that are being used in the treatment of asthma in south western nigeria .
gbolade made an inventory of plants used in the treatment of diabetes in lagos state and plants used in treating hypertension in edo state of nigeria [ 36 , 37 ] .
idu et al .   conducted an ethnobotanical survey of plants among the esan tribe of edo state , and identified 32 plant species belonging to 31 genera and 23 families which have been used for oral healthcare .
one of the goals of our research centre is to document and establish knowledge bases for chemical substances , including those derived from plant use in atm .
our previous studies have been focused on central africa [ 13 , 3941 ] , including the pharmacokinetics profiling of natural products ( nps ) derived from plant materials [ 4244 ] .
it has been our aim to extend the study to different regions and eventually to cover the entire continent of africa . due to the different works carried out by different research teams on the nigerian flora ,
the aim of this survey is to identify those plants used traditionally for the treatment of various ailments in nigeria that show promising lead - like activity .
our focus has been on the nps whose measured biological activities correlate with the use of the plant in atm . in this survey , the ethnobotanical uses of the plant species from nigeria versus the bioactivities of the derived nps are presented by plant family and in alphabetical order .
the ethnobotanical uses of plants in the above families are shown in table  1 , along with the biological activities of the most remarkable isolated nps .
the biological activities which correlate with the enthnobotanical uses of the plants have been highlighted in bold . from the alliaceae family , ajoene ( 1 ) and allicin ( 2 ) ,
were isolated from the bulb of allium sativum ( commonly known as garlic ) , a plant traditionally used in nigeria for the treatment of malaria , among diverse uses .
ajeone was active against plasmodium berghei in mice , thus validating the ethnobotanical use of the plant , meanwhile allicin was reported as a p. falciparum cysteine protease inhibitor .
it should be mentioned that rodent malaria is a well - known animal model for testing new compounds and plant extracts . however , trial in humans is decisive to identify a 
hit  as  a real hit  ; and this is a good way to assess toxicity and safety .
crinumglaucum is used in the treatment of cough , asthma , and convulsions in nigeria [ 48 , 49 ] , while crinum jagus is a plant used in traditional medicine , either singly or in a combination with chromoleana odorata and emilia prateramisa ( both of asteraceae family ) in the treatment of all forms of convulsion .
( amaryllidaceae ) , which exhibit acetylcholinesterase inhibition , the most active alkaloids isolated being hamayne ( 7 , ic50 = 250  m ) and lycorine ( 5 , ic50 = 450  m ) , while the other alkaloids were comparatively inactive , with haemanthamane ( 6 ) giving 3  % inhibition and crinamine ( 8) giving 4.4  % inhibition at 50  mg  ml ( 174  m )
. these results contrast with the positive control physostigmine which gave ic50 of 0.25  m .
thus , cholinesterase activity appears to be associated with the presence of two free hydroxy groups in this structural type of amaryllidaceae alkaloids .
crinamine has also been isolated from the aerial parts of the asian subspecies crinum asiaticum var .
the compound showed potent dose dependent inhibition ( ic50  =  2.7  m ) of hif-1 in a cell - based reporter gene assay .
the other components of the asian subspecies ( from korea ) showed no significant inhibition of hif-1 induced transcriptional activity.table  1summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; alliaceae , amaryllidaceae , annonaceae , apocynaceae , asteraceae and bignoniaceae plant familiesplant familyplant nameuse in traditional medicinepart of plant studiedactive principlemeasured activityauthor and referencesalliaceae 
 allium sativum 
 treatment of malaria , seasoning food , cleansing of blood , prevention and fighting of common cold , boost testosterone levels , regulation of blood sugar levels and as an antisepticbulb 
 1 and 2 

 antiplasmodial activity 
 adebayo et al .
amaryllidaceae 
 crinum glaucum 
 used in the treatment of cough , asthma , and convulsions .
the plant extracts have also exhibited analgesic , anti - inflammatory and antianaphylactic propertieswhole plant 
 3 , 4 and 5 
 acetylcholinesterase inhibitionokpo and adeyemi ,   houghton et al .
[ 
 50 ] 
 crinum jagus 
 treatment of all forms of convulsions and some infectious diseaseswhole plant 
 5 , 6 , 7 and 8 
 acetylcholinesterase inhibition , hif-1 inhibitionazikiwe et al .
, kim et al . annonaceae 
 enantia chlorantha 
 treatment of malaria , jaundice , dysentery , hypertension , skin , gastric and duodenal ulcers , inflammation , and liver - related diseases and to make unpainted furniture and veneersstem bark 
 9 and 10 

 antiplasmodial and antiviral activitiesadebayo et al . , bhadra and kumar , bidla et al
apocynaceae 
 picralima nitida 
 treatment of malaria , diarrhea and as a painkiller 
 stem bark , seed 
 11 , 12 , 13 , 14 , 15 , 16 and 17 

 antiplasmodial activity , antipsychotic and anxiolytic properties and known potent -opioid agonists 
 adebayo et al .
, , ezeamuzie et al . , okokon et al . , elisabetsky and costa - campos asteraceae 
 struchium sparganophora 
 treatment of malaria and measles , cutaneous , subcutaneous parasitic infection , rheumatic pains , diarrhea , dysentery as well as venereal diseases , as an abortifacient , and in the treatment of inflammatory and tumor - related ailments .
also used in the preparation of soup in the south western part of nigerialeaf 
 18 , 19 and 20 

 antimicrobial and antitumour activitieskasim et al .
bignoniaceae 
 spathodea campanulata 
 extracts of bark , leaves and flowers are used to treat malaria , hiv , diabetes mellitus , oedema , dysentery , constipation , gastrointestinal disorders , ulcers , skin diseases , wounds , fever , urethral inflammation , liver complaints and as a poison antidotestem bark 
 21 

 antiplasmodial activity , cardioprotective and aromatase inhibitoradebayo et al .
treatment of diseases ( ulcers , dysentery , oedemas , skin eruptions , scabies , wound healing and urethral discharge ) and veterinary application have been attributed to the plant in different culturesflowers , fruits , leaf and stem bark 
 22 , 23 , 24 , 25 , 26 and 27 

 antioxidant activity 
 elusiyan et al .
2active principles from allium sativum , crinum glaucum , crinum jagus , enantia chlorantha , picralima nitida , struchium sparganophora , spathodea campanulata , guiera senegalensis and morinda lucida 
  summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; alliaceae , amaryllidaceae , annonaceae , apocynaceae , asteraceae and bignoniaceae plant families active principles from allium sativum , crinum glaucum , crinum jagus , enantia chlorantha , picralima nitida , struchium sparganophora , spathodea campanulata , guiera senegalensis and morinda lucida 
  enantia chlorantha ( annonaceae ) is an ornamental tree , of up to 30 meters in height , with dense foliage and spreading crown .
it s stem bark is used against fever / malaria by traditional medicine practitioners in the forest regions , in addition to its use in the treatment of jaundice , dysentery , hypertension , inflammation , and liver - related diseases .
the isolated compounds palmatine ( 9 ) and jatrorrhizine ( 10 ) are known to exhibit anti - malarial activity , while palmatine also has weak in vitro activity against flavivirus .
seven compounds with anti - malarial properties ; akuammicine ( 11 ) , akuammine ( 12 ) , alstonine ( 13 ) , picraline ( 14 ) , picratidine ( 15 ) picranitidine ( 16 ) and -akuammigine ( 17 ) have been isolated from the stem bark and seeds of picralima nitida ( apocynaceae ) , a plant used in the treatment of malaria and in the management of pains and other ailments [ 55 , 56 ] . based on these results ,
an enterprising ghanaian hospital started manufacturing standardised 250  mg capsules of the powdered p. nitida seed , and sold around the country where they became widely accepted as a safe and effective pain relief product .
the extract showed potent and dose - dependent anti - inflammatory , anti - pyretic and anti - malarial activities . given intraperitoneally , this extract inhibited carrageenan - induced rat paw oedema with ic50 of 102  mg  kg , and with the highest dose tested ( 300  mg  kg ) producing 72.2  % inhibition . on the lps - induced pyrexia in rabbits , 50  mg  kg of the extract produced a mean percentage antipyrexia of ( 38.7  % ) compared with ( 29.0  % ) by 200  mg  kg of aspirin . in a 4-day in vivo schizontocidal test in mice infected with p. berghei , up to 300  mg  kg daily for 4  days was ineffective in preventing the development of parasitaemia or the consequent mortality .
however , marked inhibitory activity was obtained on multi - drug resistant human p. falciparium parasites cultured in vitro .
the dose causing 50  % inhibition of parasite growth was 1.75  g  ml , compared with 0.14  g  ml for chloroquine .
the results justify the use of this plant by natives of west africa in the treatment of malaria .
akuammidine and -akuammigine , are known to be potent -opioid agonists , although not particularly selective .
investigated the leaves of struchium sparganophora ( asteraceae ) , a plant used traditionally to treat malaria and measles . the isolated compounds ( 1820 ) demonstrated antimicrobial activities against the bacteria staphylococcus aureus ( nctc 6571 ) , klebsiella aerogenes ( welcome res .
lab.cn 345 ) , escherichia coli ( nctc 9001 ) and proteus vulgaris ( nctc 8313 ) , as well as against the fungal strains candida albicans ( atcc10231 ) and aspergilus niger ( ncpf3149 ) .
vernodalin ( 19 ) has been also isolated from vernonia amygdalina , along with vernomygdin , and shown to demonstrate antitumour activities .
the anti - malarial activity of ursolic acid ( 21 ) , derived from spathodea campanulata ( bignoniaceae ) , popularly known as african tulip tree , has been used to endorse the use of the plant in southwestern nigeria for malaria treatment by drinking the decoction of its stem bark .
ursolic acid also has potential use as a cardioprotective compound   and was found to be a weak aromatase inhibitor ( ic50  =  32  m ) .
additionally , the antioxidant activities of compounds 2227 isolated from the flowers , fruits , leaf and stem bark of the same plant have been investigated by elusiyan et al . .
the results show that the antioxidant principles isolated from the various parts of the plant are verminoside ( 24 ) , from the leaves , stem bark and flowers ( ec50  =  2.04  g  ml ) , specioside ( 23 ) , from the flowers ( ec50  =  17.44  g  ml ) , kampeferol diglucoside ( 22 ) , from the leaves ( ec50  =  8.87  g  ml ) and caffeic acid ( 26 ) , from the leaves and fruits .
verminoside has also been isolated from kigelia africana , a plant used in africa for anti - inflammatory , anti - microbial , and anti - skin - aging effects .
the anti - inflammatory activity of the compound has been evaluated by cutaneous irritation in cell cultures and reconstituted human epidermis and revealed significant anti - inflammatory activity .
the non antioxidant components isolated in the study include ajugol ( 25 ) , from the stem bark and fruits and phytol ( 27 ) , from the leaves .
a summary of the biological activities of the compounds that mark the ethnobotanical uses of the plants of the above families is given in table  2 .
guiera senegalensis ( combretaceae ) is often used in nigeria for the treatment of malaria .
the leaf extract of the plant harvested in nigeria showed positive anti - malarial activity in vitro in plasmodium yoelii nigeriensis .
the alkaloids harman ( 28 ) and tetrahydroharman ( 29 ) and the methoxylated naphthalene derivative guieranone a ( 30 ) , fig .  3 , were shown to be the active principles from this plant species harvested in mali and so tom [ 65 , 66 ] in addition to its antifungal activity .
the leaves of tithonia diversifola ( compositae ) have also been extensively used in nigerian traditional medicine to treat malaria and fevers .
oral decoction of the leaves of tithonia diversifolia are used for treatment of hepatitis , diabetes , malaria , pain , chemoprevention and anti - helicobacter pylori [ 6870 ] , external application of dried leaves on wounds and infusion of leaves for the treatment of measles .
the anti - plasmodial compound 31 has been proven to be the active principle responsible for the use of the plant leaves in malaria treatment .
another species from the compositae family , laggera pterodonta , is often used against insect attack , athlete s foot , skin infections , pediatric malaria and wounds .
the plant is also used in the treatment of hepatitis , arthritis , bronchitis and nephritis .
five sesquiterpenes ( 3235 ) , exhibiting antimicrobial activities , have been identified from the species growing in china .table  2summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; combretaceae , compositae , connaraceae , crassulaceae and ebenaceae plant familiesplant familyplant nameuse in traditional medicinepart of plant studiedactive principlemeasured activityauthor and referencecombretaceae 
 guiera senegalensis 
 treatment of malaria , diarrhea , dysentery , venereal diseases and microbial infections 
 leaves 
 28 , 29 and 30 

 antiplasmodial and antifungal activitiesiwalewa et al .
, silva and gomez compositae 
 tithonia diversifola 
 treatment of malaria , hepatitis , diabetes , malaria , pain , measles , chemoprevention and anti - helicobacter pylori 
 leaves 
 31 

 antiplasmodial and anti - ulcer activitieskuroda et al .
, castillo - jurez et al . , adebayo et al . , goffin et al . , snchez - mendoza et al . 

laggera pterodonta 
 against insect attack , athlete s foot , skin infections , pediatric malaria and wounds .
also used in treatment of hepatitis , arthritis , bronchitis and nephritisair - dried aerial part 
 32 , 33 , 34 , 35 and 36 

 antimicrobial activity 
 egharevba et al .
connaraceae 
 byrsocarpus coccineus 
 leaf decoction is used for the treatment of venereal diseases and as antidote to arrow poisoning and as remedy for pile , while the decoction of the whole plant is applied to swelling and tumours and also to arrest bleeding , the plant has also been reported as a remedy for diarrheaair - dried leaves 
 37 , 38 and 39 
 not testedahmadu et al .
crassulaceae 
 bryophyllum pinnatum 
 treatment of ulcers , allergic inflammation and epilepsydried whole plant 
 18 , 40 and 41 

 antibacterial activity 
 ogungbamila et al .
ebenaceae 
 diospyros mespiliformis 
 leaf decoction used for whooping cough treatment and root extracts as worm expellantsroot 
 42 
 cytotoxicityadeniyi et al .
3active principles from tithonia diversifola , laggera pterodonta , byrsocarpus coccineus , bryophyllum pinnatum and diospyros mespiliformis 
  summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; combretaceae , compositae , connaraceae , crassulaceae and ebenaceae plant families active principles from tithonia diversifola , laggera pterodonta , byrsocarpus coccineus , bryophyllum pinnatum and diospyros mespiliformis 
  the leaf extract of byrsocarpus coccineus ( connaraceae ) has been used for the treatment of several venereal diseases and as an antidote to arrow poisoning .
it also acts as a remedy for piles , while the decoction of the whole plant is applied to swellings and tumours and to arrest bleeding .
the isolated flavonoid and flavonoid glycosides ( 3739 ) , have been respectively identified as quercetin 3-o--arabinoside , quercetin and quercetin 3--d - glucoside .
the flavonoid luteolin ( 18 ) , epigallocatechin 3-o - syringate ( 40 ) and gallic acid ( 41 ) have been identified as the active principles responsible for the antibacterial activity of this plant , which explains why it is used in many west african traditional medicinal recipes for the treatment of ulcers .
the main antibacterial constituent was found to be free gallic acid , which accounted for about 0.014  %  w / w of the fresh aerial part .
however , luteolin and a new acylated flavan-3-ol , epigallocatechin-3-o - syringate , were isolated as minor constituents in the active fraction .
luteolin exhibits a wide range of biological activities , including antioxidant activity promotion of carbohydrate metabolism , and immune system modulation .
other in vitro studies suggest luteolin has antiinflammatory activity [ 77 , 78 ] , and that it acts as a monoamine transporter activator , a phosphodiesterase inhibitor , and an interleukin 6 inhibitor . in vivo studies
show that luteolin affects xylazine / ketamine - induced anesthesia in mice . in vitro and in vivo experiments
also suggest the compound may inhibit the development of skin cancer [ 81 , 82 ] .
diosquinone ( 42 ) , derived from the roots of diospyros mespiliformis ( ebenaceae ) , has shown very good activity against all the cell lines tested with ed50 values ranging between 0.18  g  ml against human glioblastoma ( u373 ) and 4.5  g  ml against hormone dependent human prostate cancer ( lncap ) .
the relationship with the ethnobotanical use of this plant has not however been established ( the leaf decoction has been used for the treatment of whooping cough and root extracts used as worm expellants ) .
amongst the plants of the euphorbiaceae family , tor - anyiin et al .   have reported the use of alchornea cordifolia in the treatment of malaria / fevers in nigeria ( table  3 ) .
.   identified ellagic acid ( 43 ) , fig .  4 , to be the active ingredient from the stem of this plant , responsible for its anti - malarial activity .
the leaves of the same plant are used as a remedy for arthritis , muscle pain and other inflammatory disorders .
carried out an analysis of the volatile oil extracted from the fresh leaves of a. cordifolia and revealed the presence of high concentrations of eugenol ( 44 : 41.7  % ) , cadinol ( 45 : 2.46  % ) , caryophylene ( 56 : 1.04  % ) , linalool ( 57 : 30.59  % ) and ( e)--bergamotene ( 59 : 4.54  % ) .
these compounds could be contributing to the topical anti - inflammatory effects of a. floribunda and a. cordifolia leaf extracts .
okoye and osadebe had also carried out a bioactivity - guided fractionation of the ethyl acetate fraction of the methanol leaf extract of the plant material , leading to the isolation of a new flavonol glycoside , 3,5,7,3-tetrahydroxyflavone-3-o--l - rhamnoside ( 61 ) .
the anti - inflammatory activity ( 50  mg  kg ) of this compound was higher than that of the standard anti - inflammatory drug , aspirin ( 100  mg  kg ) .
the compound also significantly ( p  <  0.001 ) inhibited heat - induced haemolysis of human erythrocytes in vitro .
these results demonstrated that the anti - inflammatory activity of a. floribunda leaves may be , in part , a result of the flavonol glycoside , compound 61.table  3summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from euphorbiaceae plant familyplant familyplant nameuse in traditional medicinepart of plant
studiedactive principlemeasured activityreferenceeuphorbiaceae 
 alchornea cordifolia 
 the leaves or leafy stems , as an infusion or chewed fresh , are taken for their sedative , antimalarial and antispasmodic activities to treat a variety of respiratory problems including sore throat , cough and bronchitis , genital  urinary problems including venereal diseases and female sterility , and intestinal problems including gastric ulcers , diarrhoea , amoebic dysentery and wormsstem 
 43 

 antiplasmodial activity 
 tor - anyiin et al .
remedy for arthritis , muscle pain and other acute and chronic inflammatory disorders 
 leaves 
 4451 , 5360 and 62 

 anti - inflammatory activity 
 okoye et al . 

alchornea floribunda 
 leaves are traditionally used as a remedy for arthritis , muscle pain and other inflammatory disorders 
 leaves 
 6175 

 anti - inflammatory activity 
 okoye et al . , okoye and osadebe 
 jatropha gossypifolia 
 treatment of various disease conditions such as cough , tuberculosis , bacterial infections and cancerous growths .
the leaves of the plant are traditionally being applied to boils , carbuncles , eczema , itches , and veneral diseases and also used as febrifuge , while its bark is used as emmenagogue .
the leaves and seeds are considered as a purgative and are widely used to treat obstinate constipation .
roots are used to treat leprosy , and stem latex possess coagulant activityseeds 
 76 

 antifungal activityfalodun et al .
 i summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from euphorbiaceae plant family active principles from alchornea cordifolia 
i from this same family , jatropha gossypifolia has been used to treat various disease conditions such as cough , tuberculosis , bacterial infections and cancerous growths .
the phytochemical studies revealed the presence of some secondary metabolites such as alkaloids , saponins and tannins .
there was no activity against the tested bacteria ( gram positive and gram negative organisms at 2.5100  mg  ml ) .
the seed extract however showed significant antifungal activity . from the seeds of this plant falodun et al .
carried out a spectroscopic analysis ( 1d and 2d nmr ) of the colourless oil , giving 9-acetoxynerolidol ( 76 ) , which they suggested was the active ingredient   figs .  59.fig . 
6active principles from jatropha gossypifolia , cajanus cajan , ganoderma colossum , hyptis suaveolens , cassia alata , berlina grandiflora , abrus precatorius and cassia siamea 
 fig . 
9active principles from ixora coccinea , fagara zanthoxyloides , quassia amara , quassia indica and aframomum danielli 
  active principles from alchornea floribunda  ii active principles from jatropha gossypifolia , cajanus cajan , ganoderma colossum , hyptis suaveolens , cassia alata , berlina grandiflora , abrus precatorius and cassia siamea 
  active principles from loranthus micranthus , sida acuta and ixora coccinea 
  active principles from azadiracta indica , khaya grandifoliola and pavetta crassipes 
  active principles from ixora coccinea , fagara zanthoxyloides , quassia amara , quassia indica and aframomum danielli 

our survey also involved five species from the fabaceae or leguminoceae family , table  4 .
these are cajanus cajan used in cancer treatment , cassia alata used in the treatment of skin diseases such as ringworm , eczema , pruritis , itching , scabies , ulcers and other related diseases , berlina grandiflora used in the treatment of gastrointestinal disorders , as well as abrus precatorius and cassia siamea , both used in the treatment of malaria .
a dichloromethane ( ch2cl2 ) fraction of the leaves of cajanus cajan had ic50 value 510  g  ml , with the two constituent stilbenes , longistylins a ( 78 ) and c ( 79 ) , being primarily responsible , with ic50 values of 0.714.7  m against the range of cancer cell lines [ 8991].table  4summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; fabaceae , ganodermataceae , lamiaceae and loranthaceae plant familiesplant familyplant nameuse in traditional medicinepart of plant studiedactive principlemeasured activityauthor and referencefabaceae 
 cajanus cajan 

 cancer treatment , for the treatment diabetes and as an energy stimulantleaves 
 7779 

 cytotoxicity 
 ashidi et al .
ganodermataceae ( fungus ) 
 ganoderma colossum 
 mushrooms of this genus are known to possess anti - tumour , anti - cancer , immunomodulatory and immunotherapeutic qualities 
 whole fungus 
 8082 
 antimicrobial activity , activity against hiv-1 protease 
 ofodile et al . ,
lamiaceae 
 hyptis suaveolens 
 treatment of respiratory tract infections , colds , pain , fever , cramps and skin diseasesleaves 
 83 

 antiplasmodial activity 
 chukwujekwu et al .
leguminosae 
 cassia alata 
 treatment of skin diseases such as ringworm , eczema , pruritis , itching , scabies , ulcers and other related diseasewhole plant 
 84 

 antibacterial activity 
 okwu and nnamdi 
 berlina grandiflora 
 treatment of gastrointestinal disorders 
 whole plant 
 85 

 anthelmitic activity 
 enwerem et al . 

abrus precatorius 
 treatment of malaria 
 stem bark 
 86 

 antiplasmodial activity 
 muhammad et al .
stem bark is used as a mild , pleasant , safe purgative ; to treat diabetes ; a paste is used as a dressing for ringworm and chilblains ; the roots are used as an antipyretic ; and the leaves are used for the treatment of constipation , hypertension , and insomnialeaves 
 8789 

 antiplasmodial activity , vasodialator effect 
 ajaiyeoba et al . ,
loranthaceae 
 loranthus micranthus 
 treatment of diarrhea , epilepsy , hypertension and rheumatismleafy twigs 
 9095 
 antioxidant activityagbo et al .
treatment of several diseases including immune - modifying diseases 
 leaves 
 96 and 97 

 immunostimulatory activity 
 omeje et al .  
summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; fabaceae , ganodermataceae , lamiaceae and loranthaceae plant families ofodile et al .
have isolated and characterized three colossolactones ; colossolactone e ( 80 ) , colossolactone b ( 81 ) and 23-hydroxycolossolactone e ( 82 ) , from an n - hexane : dichloromethane ( 2:7 ) extract of ganoderma colossum .
the antimicrobial screening of these compounds revealed that colossolactone e and 23-hydroxycolossolactone e were active against bacillus subtilis and pseudomonas syringae .
potency of the compounds against the tested bacteria supports the use of this mushroom in therapeutic medicine , since this genus of mushrooms is known to possess anti - tumor , anti - cancer , immunomodulatory and immunotherapeutic qualities .
other colossolactones ( v  viii and g ) and schisanlactone a have also been isolated from the mushroom , ganoderma colossum , harvested from vietnam , together with colossolactone e ( 80 ) .
the isolated compounds were evaluated for inhibition of hiv-1 protease , with ic50 values for the most potent compounds ranging from 5 to 13  g / ml .
colossolactone g showed the most promising anti - viral activity , thus justifying the immunomodulatory and immunotherapeutic qualities of this mushroom .
hyptis suaveolens ( lamiaceae ) , used in the treatment of respiratory tract infections , colds , pain , fever , cramps and skin diseases has been investigated by chukwujekwu et al . .
a bioactivity - guided fractionation of the petroleum ether extract of the leaves of this plant led to the isolation of the abietane - type diterpenoid endoperoxide , 13-epi - dioxiabiet-8(14)-en-18-ol ( 83 ) , a molecule with high anti - plasmodial activity ( ic50  =  0.1  g  ml ) .
this might explain the usefulness of the plant for treating fever , which might be due to malaria . among the other plants of the leguminoceae family ,
the antibacterial activity of 4-butylamine-10-methyl-6-hydroxy cannabinoid dronabinol ( 84 ) , isolated from cassia alata , could explain the use of this plant in the treatment of ulcers , amongst other skin diseases .
the anthelmintic activity of betulinic acid ( 85 ) was also used to validate the use of berlina grandiflora for treating gastrointestinal disorders .
the investigations of muhammad and amusa have reported the use of the stem bark of abrus precatorius ( leguminosae - caesalpinioideae ) in the treatment of malaria .
isolated abruquinone b ( 86 ) , which showed anti - plasmodial activity , with ic50  =  1.5  g  ml against the k1 strain of p. falciparum , from the stem bark of this plant .
also reported the use of the leaves and stem bark of cassia siamea in the treatment of malaria .
investigation of the leaves of this plant led to the isolation of emodin ( 87 ) , lupeol ( 88 ) and cassiarin a ( 89 ) , with ic50 values of 5.0  g  ml against the k1 strain for both compounds 87 and 88 and an ic50 value of 0.02  m for compound 90 [ 101 , 102 ] . in asian traditional folk medicine ,
the stem bark of cassia siamea is used as a mild , pleasant , safe purgative ; a decoction of the bark is given to treat diabetes ; a paste is used as a dressing for ringworm and chilblains ; the roots are used as an antipyretic ; and the leaves are used for the treatment of constipation , hypertension , and insomnia .
the vasodilator effect of cassiarin a , could explain the use of this plant in the treatment of hypertension , amongst other ailments . from the leafy twigs of loranthus micranthus ( loranthaceae )
the antioxidant activities of the isolated compounds were evaluated using the 2,2-diphenyl-1-picrylhydrazyl ( dpph ) assay , giving ic50 values varying from 23.8 and 50.1  m , all being more active than the reference drug chlorogenic acid ( with ic50  =  67.9  m ) .
the immunostimulatory activities of 2,3-dimethoxy - benzo[a , b]cyclopentenyl-3,3,5-trimethyl pyran-4-carboxylic acid ( 96 ) and lupinine ( 97 ) have been used to validate the use of the leaves of this same plant in the treatment of several diseases including immune - modulating diseases .
sida acuta ( malvaceae ) is widely used for the treatment of malaria in west africa , among other diseases .
the ethanol , aqueous and chloroform extracts are known to display antiplasmodial activity at 3.90 , 0.92 and 0.87  g  ml respectively against the p. falciparum fcm29 strain [ 106108 ] .
identified cryptolepine ( 98 ) as the active ingredient from samples collected in ivory coast , west africa .
cryptolepine analogues have also been isolated from cryptolepis sanguinolenta ( periplocaceae ) , harvested from diverse locations in west africa and exhibiting antiplasmodial activity [ 109114 ] .
different parts of azadiracta indica and khaya grandifoliola ( meliaceae ) are also used in west africa for traditional preparations to treat malaria [ 115117 ] .
the limonoid gedunin ( 99 ) , isolated from the leaves of a. indica , inhibits p. falciparum at ic50 of 1.25  g  ml [ 115 , 118 ] .
gedunin was also isolated from k. grandifoliola , along with the other limonoids 2,6-dihydroxyfissinolide ( 100 ) , methylangolensate ( 101 ) , 7-deacetylkhivorin ( 102 ) , 1-deacetylkhivorin ( 103 ) and 6-acetylswietenolide ( 104 ) , exhibiting significant antiplasmodial activities .
additionally , gedunin has displayed anticancer activities   and hsp90 inhibition . among the surveyed plants of the rubiaceae family
, pavetta crassipes has been used in handling respiratory infections and abdominal disorders , while the leaves and stems of morinda lucida are used in treating malaria [ 45 , 84 ] and ixora coccinea is used to treat a variety of infections , including hypertension , menstrual irregularities , sprains , chronic ulcers and skin diseases . a bioactive flavonoid
( quercetin-3-o - rutinoside , 105 ) has been isolated from the aqueous extract of p. crassipes leaves , which showed activity against some pathogenic microorganisms , including streptococcus pyogenes , corynebacterium ulcerans , klebsiella pneumoniae , neisseria gonorrhoeae , pseudomonas aeruginosa , and escherichia coli at a concentration < 50  mg  ml .
the compound had minimum inhibitory concentration ( mic ) ranging from 6.25 to 12.5  mg  ml and minimum bactericidal concentration ( mbc ) ranging from 12.5 to 25  mg  ml .
this supports the ethnomedicinal use of the plant in the treatment of respiratory infections and abdominal disorders .
the ethanol , ch2cl2 and petroleum ether extracts of the leaves and stems of morinda lucida exhibited respective ic50 values of 5.70 , 5.20 and 3.90  g  ml .
.   identified ursolic acid ( 21 ) as the active ingredient , which exhibited antiplasmodial activity at ic50 of 3.10  g  ml against the chloroquine - sensitive strain of p. falciparum .
identified a doubly linked , a - type proanthocyanidin trimer and other constituents of ixora coccinea leaves .
the antioxidant and antibacterial properties of the identified compounds ( 93 , 106111 ) were also investigated .
all tested compounds inhibited the growth of b. subtilis , while only epicatechin ( 93 ) and quercetin-3-o--l - rhamnopyranoside ( 110 ) inhibited the growth of e. coli .
antioxidant evaluation of isolated compounds revealed that ixoratannin a-2 ( 107 ) and cinnamtannin b-1 ( 108 ) were the most active compounds in dpph , inhibition of lipid peroxidation and nitric oxide radical scavenging assays .
the presence and antiplasmodial property of the alkaloid fagaronine ( 112 ) , with an ic50 of 0.018 m against the 3d7 strain of p. falciparum , in fagara zanthoxyloides ( rutaceae ) , could explain why the roots of this plant are used in preparations against malaria , amongst other applications in atm .
the leaves and stems of plants from the quassia species ( simaroubaceae ) are generally employed in anti - malarial preparations , q. amara ( also called bitterwood tree ) having the highest anti - malarial reputation for curative and preventive purposes in the simaroubaceae family [ 45 , 126 ] .
simalikalactone d ( 113 ) , samaderine x ( 114 ) , samaderine z ( 115 ) , samaderine b ( 116 ) and samaderine e ( 117 ) have been isolated from q. amara and q. indica , displaying anti - plasmodial properties in the range 0.010 and 0.210  g  ml [ 127 , 128 ] .
the compound displayed anti - malarial activity and was shown to be less toxic than simalikalactone d ( 113 ) .
have shown that the alcohol and petrol extracts of seeds of aframomum danielli ( zingiberaceae ) , used traditionally as a food spice and also as an anti - inflammatory agent , inhibit the soya 5-lipoxygenase enzyme and thus may show anti - inflammatory activity .
compounds isolated from the active extract were shown to be long chain polypropenyl benzoquinone derivatives .
phytyl plastoquinone ( 119 ) and heptaplastoquinone ( 120 ) were respectively isolated from the petrol and alcohol extracts , both compounds inhibiting 5-lipoxygenase at 6.25 and 18.5  m , respectively , compared with the standard drug fisetin , which inhibited the enzyme at 0.92  m , table  5.table  5summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; malvaceae , meliaceae , rubiaceae , rutaceae and simaroubaceae plant familiesplant familyplant nameuse in traditional medicinepart of plant studiedactive principlemeasured activityauthor and referencemalvaceae 
 sida acuta 
 treatment of malaria , ulcer , fever , gonorrhea , abortion , breast cancer following inflammation , wound infectionsstem 
 98 

 antiplasmodial activityobute , bertani et al .
it is also known for anthelmintic , antifungal , antidiabetic , antibacterial , antiviral , contraceptive and sedative propertiesleaves 
 99 

 antiplasmodial activityisah et al . 

extracts also show anti - inflammatory and toxic effectsbark , roots and seeds 
 99104 

 antiplasmodial and anticancer activities and hsp90 inhibitionagbenanusi et al .
rubiaceae 
 pavetta crassipes 
 management of respiratory infections and abdominal disordersfresh plant 
 105 

 antimicrobial activitybello et al . 

morinda lucida 
 treatment of malaria , diabetes , leaves , stem bark 
 20 

 antiplasmodial activityawe and makinde , cimanga et al . 

ixora coccinea 
 treatment of a variety of infections ; hypertension , menstrual irregularities , sprains , chronic ulcers and skin diseasesleaves 
 93 , 106111 

 antioxidant activitiesidowu et al .
the stem and the root of the plant are both used as chewing stick in nigeria particularly among the yoruba ethnic group in the south - western part of the countryroot 
 112 

 antiplasmodial activity 
 odebiyi and sofowora simaroubaceae 
 quassia amara 
 treatment of malaria , as a digestive , treat fever , against hair parasites ( lice , fleas ) , and mosquito larvae in ponds ( and do not harm the fishes ) .
extracts of quassia wood or bark act as a natural insecticideleaves , stem 
 113 

 antiplasmodial activity 
 ajaiyeoba et al . 

quassia indica 
 treatment of malaria , rheumatism , asthma , skin diseases and as an insecticide , especially against white antsleaves , stem 
 114118 

 antiplasmodial activity 
 bertani et al .  
zingiberaceae 
 aframomum danielli 
 used as traditional food spice , as an anti - inflammatory agent and also for crop protectionseeds 
 119 and 120 
 lipoxygenase inhibitionodukoya et al
.   summary of ethnobotanical uses versus measured biological activities of isolated secondary metabolites from ; malvaceae , meliaceae , rubiaceae , rutaceae and simaroubaceae plant families
the results presented in this review represent an overview of the biological activities of selected nps isolated from plants used in traditional medicine in nigeria .
our intention has been to focus on those plants whose ethnobotanical uses correlate with the biological activities of the derived nps .
even though this report does not claim to be exhaustive , the goal of documenting the baseline knowledge , from which further investigations could be carried out , has been achieved .
this work has also led to the collection of a chemical database for plants used in traditional medicine in nigeria ( naijaplant ) , to be made available separately .
the plant sources , geographical collection sites , chemical structures of pure compounds as well as their spectroscopic data , were retrieved from literature sources comprising data collected from 176 articles from 68 peer reviewed journals , spanning the period 1971 to 2013 .
our survey consisted in collecting data from the literature sources , mainly from msc and phd theses from nigerian university libraries and also using the author queries in major natural product and medicinal chemistry journals .
the collected data includes plant sources , uses of plant material in traditional medicine , plant families , region of collection of plant material , isolated metabolites and type ( e.g. flavonoid , terpenoid , etc . ) , measured biological activities of isolated compounds , and any comments on significance of isolated metabolites on the chemotaxonomic classification of the plant species ( as commented in the literature ) .
this survey suggests that information gathered from compounds derived from african medicinal plants , in particular from nigeria , could be a suitable starting point for extensive drug discovery projects , especially for some of the most frequently mentioned diseases like malaria , since malaria is the most endemic disease in africa and plants used in the treatment of malaria have been the most investigated on the continent [ 39 , 40 , 45 , 131134 ] .
 traditional medicinal practices play a key role in health care systems in countries with developing economies . 
 the aim of this survey was to validate the use of traditional medicine within local nigerian communities . in this review , 
 we examine the ethnobotanical uses of selected plant species from the nigerian flora and attempt to correlate the activities of the isolated bioactive principles with known uses of the plant species in african traditional medicine . 
 thirty - three ( 33 ) plant species were identified and about 100 out of the 120 compounds identified with these plants matched with the ethnobotanical uses of the plants . 
Introduction
Alliaceae, Amaryllidaceae, Annonaceae, Apocynaceae, Asteraceae and Bignoniaceae
Combretaceae, Compositae, Connaraceae, Crassulaceae and Ebenaceae
Euphorbiaceae
Fabaceae, Ganodermataceae, Lamiaceae and Loranthaceae
Malvaceae, Meliaceae, Rubiaceae, Rutaceae and Simaroubaceae
Conclusions