 tetrazine 
 ligations have proven to be a powerful bioorthogonal 
 technique for the detection of many labeled biomolecules , but the 
 ligating nature of these reactions can limit reaction turnover in 
 templated chemistry . we have developed a transfer reaction between 
 7-azabenzonorbornadiene derivatives and fluorogenic tetrazines that 
 facilitates turnover amplification of the fluorogenic response in 
 nucleic acid - templated reactions . 
 fluorogenic tetrazine - mediated transfer 
 ( tmt ) reaction probes can be used to detect dna and microrna ( mirna ) 
 templates to 0.5 and 5 pm concentrations , respectively . 
 the endogenous 
 oncogenic mirna target mir-21 could be detected in crude cell lysates 
 and detected by imaging in live cells . 
 remarkably , the technique is 
 also able to differentiate between mirna templates bearing a single 
 mismatch with high signal to background . 
 we imagine that tmt reactions 
 could find wide application for amplified fluorescent detection of 
 clinically relevant nucleic acid templates . 
Supplementary Material