the masectomized tissue of an 11-year - old female yorkshire terrier with large intestinal and abdominal tissues were obtained from a hwanggum animal medical center ( daegu , korea ) for evaluation of tumors .
a laparatomy revealed masses that were black to reddish colored and 2 - 3 mm in diameter ; they were multifocally located on the serosal membrane of the large intestine and visceral peritoneum of the sublumbar region .
the subcutaneous lesion of the right mammary gland showed a black to reddish mass with black to reddish petechia and ecchymosis .
tissues samples for light microscopy were fixed in 10% neutral buffered formalin , paraffin embedded , and stained with hematoxylin and eosin ( h&e ) .
for immunohistochemistry , tissue sections were deparaffinized in xylene , rehydrated in graded alcohol series , incubated in a solution of 0.3% hydrogen peroxide in methanol for 30 minutes and microwaved at 750w for 10 min in 10 mmol / l citrate buffer , ph6.0 .
tissue sections were washed with phosphate - buffered saline ( pbs ) and then immunostained with primary antibody .
the primary antibodies used recognized s100 protein ( diluted in 1:200 , dakocytomation , carpinteria , ca , usa ) , vimentin ( diluted in 1:100 , dakocytomation ) , protein kinase c- ( pkc- ; diluted in 1:100 , santa cruz biotechnogy , santa cruz , ca , usa ) .
the avidin - biotin - peroxidase complex ( abc ) solution of the abc kit ( vector laboratories , burlingame , ca , usa ) with 3,3-diaminobenzidine ( zymed laboratories , san francisco , ca , usa ) was used for detection .
the most extensively invaded lesions , skin and mammary glands showed abnormal hyperplasia of melanocytes in the dermal layers with hyperactivated epidermis melanocytes ( figure 1a ) .
melanocytic tumor cells had invaded into the dermal lymphatic channels ( figure 1b ) and micro vessels and were hyperpigmented in the dermal reticular layer and deeper layers .
normal mammary glands were invaded and destroyed by tumor cells ( figures 1c and 1d ) .
pleomorphic round cells were arranged in sheets or clusters . there were also myoepithelial cells exhibiting hyperplasia .
histologic evaluation of the mass in the sublumbar region revealed melanocytes intermingled with abdominal connective tissue and invasiveness of the micro - vessels ( figures 1e and 1f ) .
the neoplastic cells were fusiform and epithelioid in the peritoneum . in a section of the large intestinal mass , melanocytes had invaded the muscular layer and the metastasis of melanocytic tumor cells through the blood vessels ( figures 1 g and 1h ) .
immunohistochemically , mononuclear amelanotic cells were positive for cytoplasmic vimentin staining ( figure 2a ) and multinucleated cells containing melanin granules were positive for intranuclear and cytoplasmic s100 staining ( figure 2b ) .
the tumor cells are closely associated with the external surfaces of microvessels which expressed pkc- ( figure 2c ) and weakly positive for pkc-. our interpretation of these observations was that the multifocally invaded tumor , classified as a malignant melanoma , had at least three distinct lesions with very similar hyperplasia types and metastasis into blood vessels .
melanoma may be one of the few neoplasms in animals whose location is an important prognostic indicator in its own right .
there is no obvious relationship between histologic characteristics , including mitotic figures and pigmentation , and survival rate .
the initial definitive diagnosis of melanoma is usually done by histologic evaluation , with cytopathology used as a screen before biopsy or as an adjunct to biopsy .
morphology of the melanoma in the metastatic site is frequently similar to the primary tumor .
vascular and/or lymphatic invasion has been thought to be a direct manifestation of metastasis for progress and prognostic factors .
angiotropism in malignant melanoma has been observed in previous studies by conventional microscopy in routine tissue sections in human [ 13,16 - 19 ] . to our knowledge , there have been no reports of the origin of the potential mechanism of melanoma .
we evaluated the expression of vimentin , s100 protein and pkc-. s100 staining characteristics have also been useful in the diagnosis of canine amelanotic melanomas .
the -isozyme of pkc is widely expressed in tissues and abnormal levels are found in tissues such as proliferating pulmonary arteriolar smooth muscle cells and adventitial fibroblasts after chronic exposure to hypoxia in mammals [ 22 - 26 ] . in our study , we carefully had to distinguish angiotropism from entrapment or engulfment of vessels by the melanoma .
pkc- activation appeared in the epithelium of microvascular channels and some tumor cells at the periphery of the vessels .
there are a number of known activation signals for pkc- such as platelet - derived growth factor ( pdgf ) , vascular endothelial growth factor ( vegf ) , epidermal growth factor ( egf ) and fibroblast growth factor ( fgf ) .
although the latter feature may in fact constitute angiotropism and metastasis , we have no means at present to verify if and when such entrapment is biologically significant . from our results , there was also vascular / lymphatic invasion , which has been thought to be a direct manifestation of metastasis progress
therefore , we consider the significance of recording vascular / lymphatic invasion until further studies are better able to sort out the problems of angiotropism versus a true intraluminal tumor . although our study involved only a single case of melanoma , it is of interest that the angiotropic melanoma presented had detailed , interesting findings of histopathology and immunohistochemistry .
these results showed that black masses of the serosal membrane of the large intestine and peritoneum originated from the skin .
the metastasis of most tumors usually occurs in the lung and other solid organs in clinical cases .
this report describes the occurrence of an angiotropic metastatic melanoma in the serosal membranes of the large intestine rather than the internal regions of the solid organ .
 an eleven - year - old spayed female yorkshire terrier presented with a sublumbar mass and upon ultrasonographic examination , was revealed to have a mammary gland tumor . 
 black to reddish colored masses , located in the visceral peritoneum of the sublumbar region was observed on laparotomy with masectomy of the right side . in the laparotomy , we observed reddish masses multifocally located in the serosal membrane of the large intestine . 
 histopathologic examination of the intestinal and abdominal mass showed highly invasiveness into the muscle and metastasis of melanocytic tumor cells through the blood vessels . 
 the mammary glands showed abnormal hyperplasia of melanocytes , destruction of the normal glands by tumor cells and infiltration of some lymphocytes in the pool of melanocytic cells . 
 we have identified a malignant melanoma containing an angiotumoral complex in which tumor cells occupied a pericytic location along the microvessels with intravasation determined by immunohistochemistry for s100 protein and protein kinase c-. histologic findings in this dog lead to a diagnosis of an angiotropic metastatic malignant melanoma . 
Materials and Methods
Results
Discussion