membranous glomerulonephritis ( mgn ) is the most important cause of nephrotic syndrome in adults . among patients
> 60 years old , secondary mgn is more common , especially due to underlying malignancy . in 2009 ,
the m - type phospholipase a2 receptor ( pla2r ) was identified as the major podocyte antigen involved in idiopathic adult mgn , while anti - pla2r antibody is not detected in secondary mgn .
this patient did not have any underlying disease that could cause secondary mgn , and anti - pla2r antibody was detected in her serum , so idiopathic mgn was diagnosed .
this is the oldest reported case of idiopathic mgn , and it is discussed with reference to the literature .
in july 2014 , a 95-year - old japanese woman was admitted to our hospital with a 3-month history of bilateral edema of the lower extremities .
when she developed chronic diarrhea at 90 years of age ( in 2009 ) , laboratory tests revealed that serum creatinine ( cre ) was 1.6 mg / dl , albumin was 3.2 g / dl , and urinary protein excretion was 0.32 g / day .
laboratory tests revealed a white blood cell count of 4,600/l , a red blood cell count of 3.7110/l , hemoglobin of 11.7 g / dl , and platelet count of 21.310/l .
in addition , total protein was 6.3 g / dl , albumin was 2.4 g / dl , serum urea nitrogen was 26 mg / dl , serum cre was 1.55 mg / dl , c - reactive protein was 1.6 mg / dl , and lactate dehydrogenase was 257 iu / l ( normal : 119 - 229 iu / l ) .
immunological tests showed that antinuclear antibody , anti - double - stranded dna antibody , and u1-nuclear ribonucleoprotein antibody were all negative .
igg was 1,340 mg / dl , iga was 427 g / dl , and igm was 266 mg / dl .
the serum level of c3 was 123 mg / dl ( normal : > 86 mg / dl ) , c4 was 19 mg / dl ( normal : > 18 mg / dl ) , and ch50 was 38 u / ml ( normal : > 30 u / ml ) .
the patient was negative for hepatitis b virus antibody , hepatitis c virus antibody , and treponema antigen .
urinary protein excretion was 9.1 g / day , and the sediment contained 5 - 10 erythrocytes per high - power field .
no findings suggestive of malignancy were revealed by imaging studies such as computed tomography and ultrasonography .
serum anti - pla2r autoantibody was positive according to the method of akiyama et al . .
light microscopy of a renal biopsy specimen containing 36 glomeruli revealed global sclerosis in 22 of them ( fig .
2a ) , but there was no definite spike formation , bubbling , or thickening of the glomerular basement membrane ( gbm ) ( fig .
2b ) . in the tubulointerstitial region , atrophy or fibrosis affected 50 - 60% of the cortical tissue ( fig .
2c ) , while the interlobular arteries showed mild to moderate fibroelastic intimal thickening ( fig .
analysis of igg subclasses revealed dominant staining for igg4 ( igg4 > igg1 > igg3 > igg2 ; fig .
electron microscopy showed subepithelial electron - dense deposits ( edd ) in the gbm , but these subepithelial edd were not associated with spike - like protrusions arising from the basement membrane ( fig .
anasarca was treated with diuretics ( furosemide at 80 mg daily ) and dietary restriction ( 6 g of salt and 700 ml of water daily ) , achieving weight loss from 56.4 to 51.0 kg . because the patient did not want steroid therapy due to adverse effects such as bone disease , cyclosporin microemulsion ( neoral at 50 mg daily ) was added , and the level at 2 h after administration was titrated in the range of 600 - 1,000 ng / ml .
hemodialysis was started when cre was 6.0 mg / dl and urea nitrogen was 102 mg / dl because of exacerbation of pleural effusion and generalized edema along with an increase in weight to 58 kg at 2 months after diagnosis .
light microscopy of a renal biopsy specimen containing 36 glomeruli revealed global sclerosis in 22 of them ( fig .
2a ) , but there was no definite spike formation , bubbling , or thickening of the glomerular basement membrane ( gbm ) ( fig .
2b ) . in the tubulointerstitial region , atrophy or fibrosis affected 50 - 60% of the cortical tissue ( fig .
2c ) , while the interlobular arteries showed mild to moderate fibroelastic intimal thickening ( fig .
analysis of igg subclasses revealed dominant staining for igg4 ( igg4 > igg1 > igg3 > igg2 ; fig .
electron microscopy showed subepithelial electron - dense deposits ( edd ) in the gbm , but these subepithelial edd were not associated with spike - like protrusions arising from the basement membrane ( fig .
anasarca was treated with diuretics ( furosemide at 80 mg daily ) and dietary restriction ( 6 g of salt and 700 ml of water daily ) , achieving weight loss from 56.4 to 51.0 kg . because the patient did not want steroid therapy due to adverse effects such as bone disease , cyclosporin microemulsion ( neoral at 50 mg daily ) was added , and the level at 2 h after administration was titrated in the range of 600 - 1,000 ng / ml .
hemodialysis was started when cre was 6.0 mg / dl and urea nitrogen was 102 mg / dl because of exacerbation of pleural effusion and generalized edema along with an increase in weight to 58 kg at 2 months after diagnosis .
in 2009 , beck et al .   identified the m - type pla2r as the major target podocyte antigen in adult idiopathic mgn and showed that it was expressed by podocytes and colocalized with igg4 in glomerular immune deposits of patients with idiopathic mgn .
a pla2r band was detected in the serum of 26 patients ( mean age 48.1  14.9 years ; range 21 - 88 ) of 37 patients ( 70% ) with idiopathic mgn , while it was not found in 8 patients with secondary mgn , 22 patients with other diseases , and 30 healthy controls .
qin et al .   evaluated chinese mgn patients with the method of beck et al . , and they detected anti - pla2r autoantibodies in the serum of 49 patients ( mean age 49.2  14.9 years ; range 18 - 77 ) of 60 patients ( 82% ) with idiopathic mgn , in 1 of 20 patients with lupus - associated mgn , in 1 of 16 patients with hepatitis b - associated mgn , and in 3 of 10 patients with tumor - associated mgn .
.   investigated circulating anti - pla2r antibodies in japanese mgn patients using a highly sensitive western blotting method under nonreducing conditions with human glomerular extract ( at dilutions of 1:25 , 1:10 , and 1 ) as the primary antibody .
anti - pla2r antibodies were detected in 53 of 100 patients ( aged 67  9 years at diagnosis ) with idiopathic mgn compared to 0 of 31 patients ( aged 61  12 years ) with secondary mgn .
studied 309 patients with idiopathic mgn and reported that 74 were elderly ( 60 years old ) , with the oldest subject being 83 years old .
the incidence of chronic renal insufficiency was significantly higher in elderly patients than in younger patients ( < 60 years old ) .
only 33 of 74 elderly patients ( 46% ) received treatment , which was steroids alone in 76% , immunosuppressants alone in 9% , or a combination in 16% of patients .
the authors found no benefit of treatment in terms of the complete remission rate and the incidence of chronic renal insufficiency , so they suggested that steroid therapy should not be routinely offered to these elderly patients .
compared the clinical features and outcomes of mgn between 95 younger patients and 60 older patients aged > 60 years , including 2 patients aged 80 and 85 years .
there was a higher incidence of an identifiable cause for the nephropathy among the older patients , who were also more likely to have hypertension and more severe renal impairment than the younger patients .
abrass   reported on the treatment of elderly patients with mgn , stating that the only consistent recommendation is to avoid high - dose corticosteroids .
she also mentioned that drug dosages should be modified and monitored carefully and that elderly patients may be particularly prone to the side effects and infectious complications of immunosuppressants . when we investigated positivity for anti - pla2r antibodies at the diagnosis of idiopathic mgn among patients attending our hospital , we identified 7 patients aged 75.1  10.2 years ( range 65 - 95 ) including the present case .
our patient was older than those reported by previous authors , such as beck et al .  
( 48.1  14.9 years ; range 21 - 88 ) , akiyama et al .  
( 49.2  14.9 years ; range 18 - 77 ) . if nephrologists performed renal biopsy more frequently in elderly patients over 90 years of age with nephrotic range proteinuria , other such cases might be discovered . in conclusion
, we encountered a 95-year - old japanese woman with nephrotic - range proteinuria , and renal biopsy revealed stage 1 mgn with igg4 dominance .
this patient did not have any underlying disease that could have caused secondary mgn , and anti - pla2r antibody was positive , so idiopathic mgn was diagnosed .
corticosteroid therapy was avoided , but hemodialysis was required for generalized edema , and the patient is currently doing well on hemodialysis . this case reminds us that idiopathic mgn with anti - pla2r antibodies can occur even at the very advanced age of 95 years .
the present report adhered to the declaration of helsinki and these patients have given their consent for the case report to be published .
 a 95-year - old woman was admitted to our hospital for evaluation of bilateral lower - limb edema persisting for 3 months . 
 serum creatinine was 1.55 mg / dl , and urinary protein excretion was 9.1 g / day . 
 renal biopsy revealed stage 1 membranous glomerulonephritis ( mgn ) with immunoglobulin g4-dominant staining . 
 this patient did not have any underlying disease such as infection with hepatitis b or c virus or malignancy , and anti - phospholipase a2 receptor ( pla2r ) antibody was detected in the serum . 
 accordingly , idiopathic mgn was diagnosed . 
 corticosteroid therapy was avoided , but hemodialysis was required to treat generalized edema . 
 the patient is currently doing well . 
 this is the oldest reported case of idiopathic mgn with positivity for anti - pla2r antibody . 
Introduction
Case Presentation
Renal Biopsy
Clinical Course
Discussion
Statement of Ethics
Disclosure Statement