moxifloxacin is a new 8-methoxy - quinolone with a broad spectrum of antibacterial activity against organisms gram - positive and gram - negative bacteria , anaerobes , and atypical organism such as mycoplasma and chlamydia spp .
it has some characteristics such as a wide spectrum of bactericidal activity , a large volume of distribution , low plasma protein binding , and relatively low minimal inhibitory concentrations ( mics ) against susceptible target microorganisms [ 1 , 2 ] , the mic of moxifloxacin for mycobacterium ulcerans ranged from 0.0150.5  gml   and for s. aureus from 0.030.12  gml .
all these characteristics have made the moxifloxacin a drug of choice to treat many infectious diseases which could not be treated with older fluoroquinolones and other antimicrobials .
the disposition study gives concentration versus time profile of drug which is prime step to generate pharmacokinetics data and subsequently to establish dosage regimens .
the comparative disposition of long acting and conventional moxifloxacin helps to evaluate the difference in dosage regimens of both types of drugs and their pharmacokinetic profiles in animal body .
no such kind of study has been reported to address such a comparative issue in live stock species ; hence , the present study was conducted to investigate the comparative profile of conventional and long acting moxifloxacin in sheep after single intravenous ( iv ) administration .
five healthy adult male sheep weighing 3845  kg were used in the present study .
the sheep were housed in well - ventilated appropriately spaced animal shed and fed with good quality fodder and concentration .
all animals were acclimatized for the period of 15 days and observed clinically daily to confirm any illness or disease .
the experimental protocol was approved by iaec and all the measures for the well being of experiment animal were taken as per cpcsea guideline .  
long acting moxifloxacin ( 10% moxifloxacin in solution with l - arginine , n - butyl alcohol , and benzyl alcohol ) and conventional moxifloxacin injectable solution , moxifloxacin - base powder for standardization , were obtained from intas animal health , gujarat , india .
water , acetonitrile , and tetrabutyl ammonium hydrogen sulfate of hplc grade were purchased from s. d. fine chem .
m disodium hydrogen phosphate and hydrochloric acid of analytical grade were purchased from s. d. fine chem .
five sheep were administered conventional and long acting moxifloxacin formulation at the dose rate of 5.5 and 7.5  mgkg through intravenous route via jugular vein in crossover design , respectively .
washout period of 15 days was kept in between administration of conventional and long acting moxifloxacin .
the blood samples were collected using needle ( 26  g , 0.45  mm  13  mm ) disposable syringe ( 5  ml ) .
blood samples ( approximately 5  ml ) were collected from each treated sheep in heparin containing test tubes with the help of an intravenous catheter ( venflon ) fixed into jugular vein at 0 time ( before drug administration ) and at 0.08 ( 5  min ) , 0.25 ( 15  min ) , 0.5 ( 30  min ) , 1 , 2 , 4 , 8 , 12 , 24 , 36 , 48 , 60 , 72 , and 96  h after drug administration . plasma was separated after centrifugation of blood samples at 1660 revolutions per minute ( rpm ) for 10 minutes .
the plasma samples were transferred to cryovials ( 3  ml capacity ) and stored at 40c until assayed for long acting moxifloxacin concentration using hplc procedure .
plasma concentrations of moxifloxacin were measured using a modified hplc method previously reported by siefert et al .
the hplc ( agilent-1100 ) system was well equipped with a modal lc-9a ( gradient solvent delivery pump ) , a modal rf-551 fluorescence detector , and a modal sil-6b automatic sampler and column heater ( cto-6a ) .
plasma samples were extracted in aliquots by adding 200  l of plasma to 200  l of acetonitrile .
plasma proteins were precipitated by shaking in an ultrasonic bath followed by centrifugation for 10  min at 1660  rpm speed .
supernatant was diluted four - fold with 0.067  m disodium hydrogen phosphate buffer with ph 7.5 and transferred to hplc autosampler vials .  
the hplc separation was performed with an injection a reserve phase c18 ( supelco , 5   , 4.6  150  mm ) with using an injection volume of 50  l . the mobile phase consisted of acetonitrile ( 20% ) and tetrabutyl ammonium hydrogen sulphate solution 10  g / l ( 80% ) .
mobile phase was filtered by 0.22  m filter and degassed by sonicator and then pumped into column at a flow rate of 1.00  mlmin at ambient temperature .
the fluorescence detection was performed at excitation wavelength of 296  nm excitation and an emission wavelength of 504  nm .
the mean recovery of long acting moxifloxacin from plasma was 85.14% at 25  ng / ml . the sensitivity of long acting moxifloxacin assay was 25  ngml .
the assay was sensitive , reproducible , and linearity was observed from 0.025 to 20  gml .
the mean ( se ) plasma concentrations of conventional and long acting moxifloxacin following single - dose intravenous administration are tabulated in table 1 . mean plasma concentration versus time profile plot of both formulations of moxifloxacin is illustrated in figure 1 .
the initial plasma drug concentration of long acting moxifloxacin was 7.212  0.107  gml achieved at 0.083  h ( 5  min ) .
the lowest detectable plasma concentration of long acting moxifloxacin level as 0.016  0.001  ml was found at 72  h. the therapeutically effective concentration maintained from 0.083 to 72  h. the minimum inhibitory contraction of moxifloxacin is 0.10.5  gml .
initially , the conventional moxifloxacin plasma level was 9.971  0.901  gml recorded at 0.083  h ( 5  min ) .
the initial concentration of moxifloxacin rapidly declined to 5.596  0.901  gml at 0.167  h ( 15  min ) .
thereafter , the concentration of the drug gradually diminished and after 24  h it was not detectable in plasma .
the plasma concentrations of conventional moxifloxacin at 0.5 , 1 , 2 , 4 , 8 , 12 , and 24  h were 3.887  0.278 , 2.496  0.007 , 1.529  0.111 , 0.544  0.012 , 0.224  0.019 , 0.067  0.008 , and 0.015  0 . 
gml , respectively . thereafter , plasma moxifloxacin level diminished gradually and was not detectable after 24  h of drug administration .  
the initial plasma drug concentration of long acting moxifloxacin was 7.227  0.13  gml achieved at 0.083  h ( 5  min ) , which rapidly declined to 5.646  0.044  gml at 0.166  h ( 15  min ) .
the plasma concentrations of long acting moxifloxacin at 0.5 , 1 , 2 , 4 , 8 , 12 , 24 , 36 , 48 , 60 , and 72  h were 4.283  0.22 , 2.853  0.136 , 2.230  0.055 , 1.712  0.077 , 0.772  0.022 , 0.368  0.019 , 0.150  0.002 , 0.076  0.002 , 0.043  0.001 , 0.026  0.001 , and 0.016  0.002  gml , respectively .
thereafter , plasma long acting moxifloxacin level diminished gradually and was not detectable at 96  h of drug administration .  
while conventional moxifloxacin administration resulted in rapid decline in plasma concentration and the drug was not detectable after 24 hours of administration .
the long acting moxifloxacin administration provided effective therapeutic concentration for up to 72 hours after single - dose administration .
this dose is advantageous with respect to cost of treatment and compliance to animal owner .
conversely , while applying long acting moxifloxacin in sheep , initially the levels of drug remained low ( 7.227  gml at 0.083  h ) as compared to its counter formulation ( conventional ) that reached initially to 9.917  gml ( 0.083  h ) . since moxifloxacin is a new generation fluoroquinolone antimicrobial drug , with some added quality of initial speedy high level with both types of formulations along with its low mic level , will definitely make this compound as the drug of choice for the treatment of infectious disorders in sheep .
 this study describes disposition of long - acting moxifloxacin and conventional formulations of moxifloxacin in sheep after intravenous administration in five male sheep . 
 long acting moxifloxacin solution ( 10% moxifloxacin in solution with l - arginine , n - butyl alcohol , and benzyl alcohol ) and conventional moxifloxacin ( 10% ) were injected in jugular vein . 
 blood samples were collected from contralateral jugular vein in test tubes containing 3050  iu heparin ( anticoagulant ) periodically from 0.083 to 72  h of drug administration . 
 drug concentrations in plasma were determined using high - performance liquid chromatography ( hplc ) with fluorescence detector . the mobile phase consisted of a mixture of buffer ( 10  gm of tetrabutyl ammonium hydrogen sulphate per liter - deionised water ) and acetonitrile ( 80  :  20 ) . 
 the buffer was 0.067 m of disodium hydrogen phosphate with ph of 7.5 . 
 the flow rate was 1  mlmin1 at ambient temperature . 
 the effluent was monitored at 296  nm excitation and 504  nm emissions wavelength . 
 hplc with fluorescence detector method for plasma moxifloxacin assay was standardized with specific modification for plasma of sheep in the present study . 
 after single - dose intravenous administration of long acting moxifloxacin the plasma concentration of 0.016  0.001  gml1 was maintained for up to 72  h. conventional formulation of moxifloxacin remained in body for up to 24  h of drug administration with the level of 0.015  0.005 gml1 . 
1. Introduction
2. Materials and Methods
3. Results and Discussion