bacteria were found in dentinal tubules of root canal dentine in histological sections of infected teeth and apical portion of root canal is an area of interest that may harbor a critical level of microorganisms , which is effective on apical periodontitis .
although cleaning of recessed surfaces is based more on irrigation , mechanical debridement at all circumferences of the apical root canal region is an important goal for endodontic instrumentation .
horizontal dimension of the preparation at its most apical extend is a critical parameter that the clinician must determine for preparation . in early literature ; according to recommendations of weine , enlargement of canals three sizes larger than the first file to bind was enough for apical preparation .
the aim of this procedure was making up an apical stop to reduce the leakage and material extrusion .
however , morphologic analyses have shown that apical foramen often does not have a round shape and to achieve a more accurate estimate of the apical diameter , removal of the interferences along the coronal and middle thirds of the root canal is necessary .
coronal flaring may eliminate the coronal interferences of the narrow and irregular canals and provide a reliable apical adaptation to instruments .
recently , rotary ni - ti instrumentation systems are popular for the preparation of root canals and most of these systems suggest coronal flaring before apical shaping with finisher instruments .
apical adaptation of the first instrument can be a reference for determining the apical preparation size and it is important in terms of best possible debridement of the infected root canals .
the aim of this study was to evaluate the adaptation of first hand files ( k , h file ) that bind at working lengths ( wls ) and first finisher rotary instruments ( protaper , profile ) that reach to wl after coronal flaring .
a total of 40 freshly extracted single - rooted mandibular incisors were selected for the study .
teeth , which have complicated anatomy , external resorption or canal curvature more than 10 were excluded .
the crowns of all teeth were removed from cemento - enamel junction to get an easy access and flat reliable reference point for length measurements .
pulp tissues were removed with barbed broaches and root canal of each tooth was explored with a size 8 k - file until the tip of the file was just visible at the apical foramen .
protaper ( dentsply maillefer , ballaigues , switzerland ) , profile ( dentsply maillefer ) , k - file ( mani , tokyo , japan ) and hedstroem ( mani ) files were used to evaluate apical binding .
2 and 1 gates - glidden burs for hedstroem ( h - file ) and k - files .
after irrigation of the canals with 5 ml naocl one investigator passively inserted increasing sizes of files started with size 10 until sense of binding . when binding was occurred only a bit short of wl one balanced force motion performed without apical pressure to reach to wl .
protaper rotary instrumentation group : sx , s1 and s2 files were used to the wl respectively and f1 or f2 files were the first finisher files that reached the wl with a sense of apical resistance .
apical stop preparation was checked by the sense of apical resistance with hand usage of the last file at wl to see apical binding of the last former instrument . for profile group 6% tapered no .
30 and 25 sequence was followed with a crown down manner according to manufacturers recommendations .
apical stop preparation was checked with the hand usage of the last file and if there was no apical resistance sense aforementioned profile sequence was used backwards until sense of binding with hand usage .
each canal was irrigated with 2 ml of 2.5% naocl after each instrument for the rotary instrumentations .
the apical 1 mm of each root tip was ground on wet sandpaper in order to expose the canal and the instrument at the wl .
apical debris was removed with an ultrasonic cleaner ( e15h , elmasonic , elma hans schmidbauer co. , singen germany ) and the apical region of each tooth was examined under stereomicroscope ( leica m60 , leica microsystems gmbh , wetzlar , germany ) at a 40 magnification [ figure 1 ] .
the pixel counts of the apical images were used for calculations on adobe photoshop image analyzing program ( cs2 version 9.0 ; adobe systems , san jose , ca , usa ) .
the differences between the area of root canals and file tips were described as gap areas and calculated with subtraction of the file area from root canal area .
the discrepancy between mean gap areas were analyzed using one - way analysis of variance test ( p = 0.05 ) .
samples from stereoscopic images , ( a ) k - file , ( b ) hedstroemem , ( c ) profile , ( d ) protaper
apical areas of the first files to bind and surrounding gap areas are shown in table 1 .
there were big gap areas ( 78.23% ) on apical image sections for total of the evaluated apical stereoscopic images .
the mean gap areas and gap percentages for the instrument groups were as follows ; k - file : 9999 , 4  3176 , 537 ( 76.53% ) , h - file : 12260 , 6  4795 ( 81.25% ) , profile : 10045 , 5  4409 , 256 ( 80.25% ) , protaper : 8883 , 3  3215 , 342 ( 74.91% ) . protaper showed the lowest mean gap area rate between evaluated instruments ; however , the differences were not statistically significant ( p > 0.05 ) .
mandibular incisors have a high incidence of isthmuses and are narrow in the proximal direction . furthermore , apical root canals of mandibular incisors are oval and irregular shaped .
this was the main reason that mandibular incisor teeth have been chosen in this study .
the root canal curvature controls the direction of the file and avoids a sufficient preparation of the apical part of the canal .
canal curvature beyond 10 is classified as moderate ( 10 - 20 ) or aggressive ( 20 - 75 ) according to schneider .
preflaring of the coronal and middle - thirds of the root canal has been recommended prior to determining the initial file that binds .
the dentine layer surrounding the apex and the root canal surfaces probably have been infected and must be instrumented after determination of the first binding file .
however , the first file that binds at the root apex not properly reflects the diameter of the apical canal . in an experimental study weiger et al .
some other experimental studies determined wl by subtracting 1 mm of the length of a small file whose tip was just visible at the apical foramen .
the aim of canal preparation is to widen the apical canal enough for irrigation and obturation procedures .
however , widening and shaping processes are crucial steps for endodontic treatments because excess instrumentation can easily weaken the root and increase the risk of fracture as well as insufficient instrumentation can cause reinfection .
the first binding file generally adapted to one side of the apical root canal , therefore apical stop may be formed only at one side .
this one sided shelf may prevent the first binding file from apical movement , but it is uncertain that it can reduce leakage and material extrusion . using this information ,
the decision should be made respecting shape and diameter of the apical foramen and root canal anatomy for a successful endodontic treatment .
if the apical region is round , the first file that binds at the wl will most likely wear the entire canal perimeter with three more file diameters .
consequently , the objective of removing the infected dentin layer and preparing a round regular shaped apical stop might not be accomplished .
considering the fact that the first file to bind frequently bound at one side of canal wall , the apical stop may be created only on one side .
however , the results of this study have shown that even the first file binds the apical foramen did not reflect the apical diameter and the size of the file was relatively smaller than the exact size of apical foramen . therefore , removing the infected dentine layer at the apical third of the root canal and preparing a round regular shaped apical stop might not be achieved without reshaping the apex .
the similar and statistically insignificant results could be explained because of the non - circular and irregular shapes of the mandibular incisors root canals and the discrepancy between root canals of the mandibular incisors .
 objective : the aim of this study was to evaluate the apical root canal adaptation performance of various root canal instruments.materials and methods : a total of 40 freshly extracted single - rooted mandibular incisors were used in this study . 
 coroner parts of all teeth were removed from cemento - enamel junction and root canal of each tooth was explored with a size 8 k - file until the tip of the file was just visible at the apex . working lengths ( wls ) were determined as 1 mm short of these measurements . 
 protaper , k - file , profile and hedstroem files were inserted into the root canals of 10 teeth to the wl following the flaring of the coronal and middle thirds . 
 instruments were fixed in the root canals with acrylic resin . 
 the apical 1 mm of each root tip was ground on wet sandpaper to expose the canal and the instrument at the wl and the apical region of each tooth was examined under stereomicroscope . 
 the stereoscopic images of the teeth were digitized and analyzed with software in order to determine the differences between the areas of root canals and file tips . 
 result data were analyzed using the one - way analysis of variance test ( p = 0.05).results : there were no significant differences between apical file / root canal areas of the evaluated instruments ( p > 0.05).conclusions : none of the evaluated instruments performed a perfect adaptation with the apical root canal surface at the wl in mandibular incisors . 
 therefore , total removal of the debris from the apical canal surface may not be achieved when these filing instruments are used . 
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONCLUSION