acute retinal necrosis ( arn ) syndrome is clinically characterized by peripheral necrotizing retinitis , retinal arteritis , and a prominent inflammatory reaction in the vitreous and anterior chamber.1 the pathogenesis of arn is presumed to be associated with herpes virus infection , varicella - zoster virus ( vzv ) , herpes simplex virus ( hsv ) , cyto - megalovirus ( cmv ) , or epstein  barr virus ( ebv).2 polymerase chain reaction ( pcr ) is a sensitive and specific method for detecting viral dna and is usually performed to assist in the diagnosis of arn from the vitreous or the aqueous humor.3 herpes zoster ophthalmicus has been linked to vzv reactivation within the trigeminal ganglion , and asymptomatic viral shedding of vzv from saliva and tear fluid has been reported.46 however , asymptomatic viral shedding in patients with arn has not been described . in the present case report
, we simultaneously identified the vzv and cmv genomes in the aqueous humor , tears , saliva , and swabs of the auricular and forehead skin areas in sequential samples assessed by pcr in an otherwise healthy patient with arn .
a 65-year - old otherwise healthy japanese woman presented at another hospital with blurred vision in the left eye .
the left eye showed inflammation in the anterior chamber and white patches in the posterior pole .
she was given a full medical examination , and the results of blood tests , including angiotensin converting enzyme , syphilis test , chest x - ray , tuberculin skin test , and cerebrospinal fluid examination , were all within normal limits .
her decimal visual acuity on the left eye was 0.15 and her intraocular pressure was 13 mmhg . on examination , there were 2 + aqueous cells in the anterior chamber and mutton - fat keratic precipitates , and the following were also observed : 3 + vitreous cells , vasculitis in the temporal arcade , and white patches of retinal necrosis in the temporal part of the posterior pole and peripheral retina ( figure 1 ) .
the right eye was normal . according to these findings and the rapid progression of necrosis , a clinical diagnosis of arn
serum vzv , hsv , and cmv eia - igm antibody levels were not detected .
serum eia - igg antibody levels were hsv 4.0 , vzv 35.2 , and cmv 3.7 arbitrary units ml , respectively .
antibody test was negative and she had no previous history of diabetes mellitus , leukopenia , cytotoxic chemotherapy , corticosteroid use , or non - cytotoxic immunosuppressive medication use . her full blood count , glucose , renal function , and liver function tests were within normal limits .
the patient was admitted to our hospital , and intravenous acyclovir 500 mg three times a day , oral prednisone 40 mg / day , and aspirin 100 mg / day were given .
recent reports have described that prophylactic vitrectomy may help to eliminate inflammatory factors and reduce the incidence of retinal detachment;7,8 we therefore performed prophylactic vitrectomy , entire circumferential scleral buckling , silicone oil tamponade , and cataract surgery on day 2 of admission .
the undiluted vitreous was sampled from the patient at the beginning of the vitrectomy and tested by pcr .
the results of the pcr of vitreous were positive for vzv and cmv and negative for hsv , ebv , and human herpes virus 6 and 7 ( figure 2 ) .
although cmv was positive , there were fewer copies than for vzv , and since the patient was otherwise healthy , we continued treatment with intravenous acyclovir . although the patient was given only acyclovir , the white patches gradually disappeared or cicatrized ( figure 3 ) .
an intravenous acyclovir dose of 500 mg three times a day was given for 12 days .
on day 12 of admission , the visual acuity of the left eye improved to 0.3 and the intraocular pressure was 10 mmhg .
the treatment was later changed to oral valacyclovir 1,000 mg , three times a day for 3 months , and oral prednisone was tapered slowly over 3 months . at 1 month after hospitalization , rhegmatogenous retinal detachment of the peripheral retina occurred and a reoperation was performed .
there was neither a new focus of infection nor a relapse of inflammation following the reoperation . at the end of the follow up ,
the visual acuity of the left eye was 0.1 and intraocular pressure was 12 mmhg .
swabs of the auricular and forehead skin areas and tears were collected bilaterally and sequentially , and pcr was performed at various time points during the treatment period . nucleic acid from clinical samples was extracted and purified using a qiaamp dna blood mini kit ( qiagen nv , venlo , the netherlands ) for amplification of the hsv-1 , hsv-2 , vzv , ebv , cmv , hhv ( human herpes virus)-6 , and hhv-7 gene by pcr , as described previously.6,9,10 pcr analysis was performed using the primer pairs shown in table 1 .
the vzv genomes were detected in the vitreous and saliva , and the swabs of the auricular and forehead skin areas .
the left eye was affected and the viral copy numbers were more abundant in the tears and skin taken from the ipsilateral side than in those from the contralateral side on days 25 , although the difference decreased with time .
cmv was detected in the tears , saliva , and skin from the initial samples with an increasing copy number on day 8 and later , but no corneal or skin lesions were observed over the observation period .
arn is caused by hsv and vzv in otherwise healthy patients and by cmv in immunosuppressed patients , but simultaneous detection of cmv has not been reported in patients with either hsv - induced arn or vzv - induced arn .
lau et al reported that the ebv - positive eyes of their patients were also positive for vzv.11 to our knowledge , this is the first case report of simultaneous detection of cmv in a patient with vzv - induced arn .
ocular cmv infections usually present as anterior uveitis.12 arn is usually caused by vzv and hsv , and more rarely by cmv in some immunocompetent patients.2 tajunisah et al reported the case of an immunocompetent patient with cmv arn in whom the vitreous tap was positive for cmv without detecting vzv and hsv.13 in their case , the patient had anterior chamber inflammation , marked vitritis , and anterior retinal necrosis .
initially , they treated the patient with 500 mg of intravenous acyclovir three times daily , but the necrotic retinitis lesions enlarged .
schneider et al presented five cases of cmv necrotizing retinitis in non - hiv patients.14 they described that the cases had both characteristic arn and classic cmv retinitis , in addition to panretinal occlusive vasculitis , moderate intraocular inflammation , and slowly progressive granular retinitis . these cases were treated with ganciclovir or valganciclovir , but they required prolonged antiviral therapy to achieve a complete resolution of retinitis , which contrasts with the rapid treatment response normally observed in the cases of arn caused by hsv or vzv .
in our case , the patient had inflammation in the anterior chamber , vitritis , occlusive vasculitis , and retinal necrosis in the temporal region of the posterior pole and the peripheral retina .
the retinal necrosis expanded within 1 week , unlike the slowly progressive retinitis described by schneider et al.14 although previous studies of cmv necrotizing retinitis have reported successful treatment with ganciclovir or valganciclovir , in our patient the retinal lesion was improved only by acyclovir , which elicited a rapid treatment response . in consideration of these clinical findings and the responses to acyclovir ,
we conclude that cmv did not contribute to the pathogenesis of arn in this case .
as shown in table 2 , the viral dna was detected by pcr even during antiviral therapy .
the inhibition of dna replication might not have been complete in the infected cells because the inhibition of dna synthesis depends on the ratio between synthesized guanosine triphosphate and incorporated acyclovir triphosphate .
vzv dna was detected in the vitreous , tears , saliva , and skin , indicating that vzv reactivation occurred in the tears , saliva , and skin of the auricular and forehead regions , a broad area of the skin and mucous membranes of the face , as shown in table 2 .
since the patient did not present any lesions related to vzv or cmv except in the left eye , asymptomatic shedding of vzv and cmv might have occurred in the facial area .
if so , such shedding might suggest that some stimuli caused viral reactivation in the various ganglia focusing on the left trigeminal ganglion .
we could not find any specific trigger in the patient history , but we simultaneously detected vzv and cmv in the vitreous and in the facial area in the patient with vzv - induced arn .
however , while the vzv reactivation caused arn in the left eye , it caused no skin lesions .
the cause of the vzv reactivation was unclear , although it might have been due to a breakdown of the immune system .
this suggests that asymptomatic reactivation of vzv and cmv might occur in patients with arn .
 backgroundto report a case of simultaneous detection of cytomegalovirus ( cmv ) in acute retinal necrosis ( arn ) due to varicella - zoster virus ( vzv ) in an immunocompetent adult.methodsa 65-year - old healthy woman presented with necrotizing retinitis . 
 vitreous , tears , saliva , and swabs of the auricular and forehead skin areas were collected and tested by polymerase chain reaction analysis at various time points during the treatment period.resultsvzv and cmv dna were detected in the vitreous , tears , saliva , and skin . 
 cmv was present in fewer copies than vzv in the vitreous . 
 the retinal lesion clinically improved rapidly only by acyclovir . according to the clinical findings and the clinical responses to acyclovir 
 , cmv seems not to cause arn in this case.conclusionvzv and cmv dna were detected in the vitreous of a patient with vzv arn . 
 cmv reactivation appeared to be asymptomatic . 
Introduction
Clinical course of the patient
Sample collection and DNA analyses
Discussion
Conclusion