the prevalence of distal symmetric polyneuropathy ( dsp ) is greater than 3% and is even higher in the elderly ( 1,2 ) .
this common condition causes significant morbidity and is associated with pain , falls , and reduced quality of life ( 3,4 ) . while diabetes is by far the number one cause , enhanced glucose control is only marginally effective at reducing the incidence in patients with type 2 diabetes ( 5 ) .
one possible explanation for this small therapeutic effect is that other factors , such as the other components of the metabolic syndrome ( mets ) , may be significant contributors to nerve injury ( 6 ) .
the other mets components may also be the underlying cause for some of the 30% of dsp patients currently labeled as idiopathic ( 710 ) .
past studies have revealed an association between the mets and polyneuropathy ( 1114 ) . however , previous investigations have reported conflicting associations between the individual mets components and neuropathy ( 1521 ) .
for example , while diabetes is a well - established risk factor for neuropathy , population studies investigating the effect of prediabetes have reached different conclusions ( 16,22,23 ) .
to date , most studies investigating the association between the mets and polyneuropathy have been performed only in patients with diabetes , used cross - sectional designs , and lacked rigorous definitions of dsp such as only evaluating one confirmatory test ( 6 ) .
the goal of this study is to address these limitations . in a large cohort of older adults , we aimed to determine whether the prevalence of symptomatic dsp , utilizing a rigorous definition , increases with increasing numbers of mets components independent of glycemic status .
we also investigated the associations between the individual mets components and symptomatic dsp in cross - sectional and longitudinal analyses .
data for our analysis came from participants in the health , aging , and body composition ( health abc ) study , which is an ongoing , prospective cohort study of 7079 year olds ( n = 3,075 ; 48.4% male ; 41.6% black ) followed since the baseline evaluation in 1997 or 1998 .
the cohort consists of a random sample of age - eligible white medicare beneficiaries , all black medicare beneficiaries , and all community - dwelling black persons in pittsburgh and memphis .
participants had to self - report having no difficulty with walking 1/4 mile , climbing 10 steps , or any basic activity of daily living ; be free of any life - threatening cancers ; and plan to remain in the study area for at least 3 years .
this cohort has been followed annually for the past 17 years with questionnaires , biospecimens , anthropomorphic measures , blood pressure , and neuropathy outcome assessments at differing intervals .
participants were included in the cross - sectional analysis if they had complete neuropathy outcome data from year 4 in 20002001 .
participants were included in the longitudinal analysis if they had complete neuropathy outcome data from years 4 ( 20002001 ) and 11 ( 20072008 ) .
all study protocols were approved by institutional review boards at the university of pittsburgh and university of tennessee health science center .
diabetes ( fasting glucose 126 mg / dl or 2-h glucose 200 mg / dl ) and prediabetes ( fasting glucose 100 mg / dl or 2-h glucose 140 mg / dl ) were defined according to the guidelines of the expert committee on the diagnosis and classification of diabetes mellitus ( 24 ) . participants on diabetes medications were also considered to have diabetes .
lipid panels and blood pressure were measured at years 1 , 2 , 4 , 6 , 8 , 10 , and 11 , with blood pressure also measured at years 3 and 5 .
height , weight , and bmi were measured at years 1 , 4 , 6 , 8 , 10 , and 11 , whereas waist circumference was only measured at years 1 and 6 .
the updated national cholesterol education program / adult treatment panel iii criteria were used to define the mets and its individual components , which takes into consideration medications for the different components ( 25 ) .
specifically , the mets criteria were a waist circumference 102 cm in men and 88 cm in women , systolic blood pressure 130 mmhg or diastolic blood pressure 85 mmhg , triglycerides 150 mg / dl , and hdl < 40 mg / dl in men and < 50 mg / dl in women . in the cross - sectional analysis ,
metabolic data from year 1 were used . in the longitudinal analysis , baseline metabolic data were obtained from year 1 with the exception of hemoglobin a1c , which was only available starting in year 4 .
our primary dsp outcome measure was determined by the combination of neuropathy symptoms as identified by at least one of two questionnaire items endorsed and at least one of three confirmatory neuropathy tests abnormal ; therefore , our primary outcome construct was symptomatic dsp .
the questionnaire items were as follows :  in the past 12 months , have you ever had numbness , an 
asleep feeling, a prickly feeling or tingling in your legs or feet ?  and  in the past 12 months , have you ever had a sudden stabbing or burning pain , or a deep aching in your legs or feet ?  the three confirmatory neuropathy tests included a peroneal motor nerve conduction velocity ( cv ) < 40 m / s , a vibration threshold > 131 m , and inability to feel a 10-g ( heavy ) monofilament at the dorsum of the great toe in three out of four trials .
in addition , the peroneal compound motor action potential ( cmap ) at the ankle and a 1.4-g ( light ) monofilament at the dorsum of the great toe ( abnormal if unable to feel in three of four trials ) were secondary outcomes .
five of the six secondary dsp outcome measures did not require symptoms ; therefore , these measures had the potential to capture asymptomatic as well as symptomatic dsp .
the peroneal motor nerve conduction studies were performed on a neuromax 8 ( xltek ) .
vibration detection was performed at the bottom of the great toe using the vsa-3000 vibratory sensory analyzer ( medoc ) .
feet were warmed to 30c , and measures were performed on the right unless contraindicated owing to knee replacement , amputation , trauma , ulcer , or recent surgery , in which case the left side was tested unless also contraindicated .
one - way anova ( continuous variables ) and  tests ( categorical variables ) were used to test for differences in demographics between the three glycemic groups .
we determined the prevalence of neuropathy stratified by glycemic status and the number of mets components .
we then applied the cochran - mantel - haenszel test to investigate the effect of the number of mets components on neuropathy prevalence , controlling for glycemic status .
multivariable logistic regression was used to model the year 4 primary neuropathy outcome as a function of the year 1 mets components ( waist circumference , prediabetes , diabetes , hdl level , triglyceride level , systolic blood pressure ) after adjustment for baseline demographic factors ( age , sex , race , height , smoking , and alcohol consumption ) .
weight , hemoglobin a1c , log homa of insulin resistance , and diastolic blood pressure were also considered as mets component variables but were not used in the final model ( akaike information criterion was used in model selection ) .
secondary neuropathy outcomes including neuropathic symptoms , heavy monofilament , and light monofilament outcomes were also analyzed using multivariable logistic regression models .
multivariable linear regression was used to analyze the continuous neuropathy measurements ( peroneal cv , peroneal cmap , vibration threshold ) as a function of mets components , adjusting for the same covariates .
finally , we fit a separate multivariable logistic regression model to study the association of the number of mets components , excluding glycemic status , with our primary neuropathy outcome , adjusting for demographic factors and glycemic status .
we first estimated the longitudinal change in each mets component ( hemoglobin a1c , weight , systolic blood pressure , triglycerides , and hdl ) by fitting a simple linear regression model over time for each subject .
then , the estimated subject - specific slopes , along with the baseline measurements for each mets component , were incorporated into a multivariable logistic regression model to investigate the association of longitudinal change in mets components and incident symptomatic neuropathy ( those with neuropathy in year 11 but not at year 4 ) after adjustment for baseline demographics as described above .
data for our analysis came from participants in the health , aging , and body composition ( health abc ) study , which is an ongoing , prospective cohort study of 7079 year olds ( n = 3,075 ; 48.4% male ; 41.6% black ) followed since the baseline evaluation in 1997 or 1998 .
the cohort consists of a random sample of age - eligible white medicare beneficiaries , all black medicare beneficiaries , and all community - dwelling black persons in pittsburgh and memphis .
participants had to self - report having no difficulty with walking 1/4 mile , climbing 10 steps , or any basic activity of daily living ; be free of any life - threatening cancers ; and plan to remain in the study area for at least 3 years .
this cohort has been followed annually for the past 17 years with questionnaires , biospecimens , anthropomorphic measures , blood pressure , and neuropathy outcome assessments at differing intervals .
participants were included in the cross - sectional analysis if they had complete neuropathy outcome data from year 4 in 20002001 .
participants were included in the longitudinal analysis if they had complete neuropathy outcome data from years 4 ( 20002001 ) and 11 ( 20072008 ) .
all study protocols were approved by institutional review boards at the university of pittsburgh and university of tennessee health science center .
diabetes ( fasting glucose 126 mg / dl or 2-h glucose 200 mg / dl ) and prediabetes ( fasting glucose 100 mg / dl or 2-h glucose 140 mg / dl ) were defined according to the guidelines of the expert committee on the diagnosis and classification of diabetes mellitus ( 24 ) . participants on diabetes medications were also considered to have diabetes .
lipid panels and blood pressure were measured at years 1 , 2 , 4 , 6 , 8 , 10 , and 11 , with blood pressure also measured at years 3 and 5 .
height , weight , and bmi were measured at years 1 , 4 , 6 , 8 , 10 , and 11 , whereas waist circumference was only measured at years 1 and 6 .
the updated national cholesterol education program / adult treatment panel iii criteria were used to define the mets and its individual components , which takes into consideration medications for the different components ( 25 ) . specifically , the mets criteria were a waist circumference 102 cm in men and 88 cm in women , systolic blood pressure 130 mmhg or diastolic blood pressure 85 mmhg , triglycerides 150 mg / dl , and hdl < 40 mg / dl in men and < 50 mg / dl in women . in the cross - sectional analysis , metabolic data from year 1 were used . in the longitudinal analysis
, baseline metabolic data were obtained from year 1 with the exception of hemoglobin a1c , which was only available starting in year 4 .
our primary dsp outcome measure was determined by the combination of neuropathy symptoms as identified by at least one of two questionnaire items endorsed and at least one of three confirmatory neuropathy tests abnormal ; therefore , our primary outcome construct was symptomatic dsp .
the questionnaire items were as follows :  in the past 12 months , have you ever had numbness , an 
asleep feeling, a prickly feeling or tingling in your legs or feet ?  and  in the past 12 months , have you ever had a sudden stabbing or burning pain , or a deep aching in your legs or feet ?  the three confirmatory neuropathy tests included a peroneal motor nerve conduction velocity ( cv ) < 40 m / s , a vibration threshold > 131 m , and inability to feel a 10-g ( heavy ) monofilament at the dorsum of the great toe in three out of four trials .
in addition , the peroneal compound motor action potential ( cmap ) at the ankle and a 1.4-g ( light ) monofilament at the dorsum of the great toe ( abnormal if unable to feel in three of four trials ) were secondary outcomes .
five of the six secondary dsp outcome measures did not require symptoms ; therefore , these measures had the potential to capture asymptomatic as well as symptomatic dsp .
the peroneal motor nerve conduction studies were performed on a neuromax 8 ( xltek ) .
vibration detection was performed at the bottom of the great toe using the vsa-3000 vibratory sensory analyzer ( medoc ) .
feet were warmed to 30c , and measures were performed on the right unless contraindicated owing to knee replacement , amputation , trauma , ulcer , or recent surgery , in which case the left side was tested unless also contraindicated .
one - way anova ( continuous variables ) and  tests ( categorical variables ) were used to test for differences in demographics between the three glycemic groups .
we determined the prevalence of neuropathy stratified by glycemic status and the number of mets components .
we then applied the cochran - mantel - haenszel test to investigate the effect of the number of mets components on neuropathy prevalence , controlling for glycemic status .
multivariable logistic regression was used to model the year 4 primary neuropathy outcome as a function of the year 1 mets components ( waist circumference , prediabetes , diabetes , hdl level , triglyceride level , systolic blood pressure ) after adjustment for baseline demographic factors ( age , sex , race , height , smoking , and alcohol consumption ) .
weight , hemoglobin a1c , log homa of insulin resistance , and diastolic blood pressure were also considered as mets component variables but were not used in the final model ( akaike information criterion was used in model selection ) .
secondary neuropathy outcomes including neuropathic symptoms , heavy monofilament , and light monofilament outcomes were also analyzed using multivariable logistic regression models .
multivariable linear regression was used to analyze the continuous neuropathy measurements ( peroneal cv , peroneal cmap , vibration threshold ) as a function of mets components , adjusting for the same covariates .
finally , we fit a separate multivariable logistic regression model to study the association of the number of mets components , excluding glycemic status , with our primary neuropathy outcome , adjusting for demographic factors and glycemic status .
we first estimated the longitudinal change in each mets component ( hemoglobin a1c , weight , systolic blood pressure , triglycerides , and hdl ) by fitting a simple linear regression model over time for each subject .
then , the estimated subject - specific slopes , along with the baseline measurements for each mets component , were incorporated into a multivariable logistic regression model to investigate the association of longitudinal change in mets components and incident symptomatic neuropathy ( those with neuropathy in year 11 but not at year 4 ) after adjustment for baseline demographics as described above .
of participants in the health abc cohort , 2,382 had complete neuropathy outcome data and were included in our cross - sectional analysis . of these 2,382 , 1,263 participants also had complete neuropathy outcome data at year 11 and were included in our longitudinal analysis . in a comparison of year 4 with year 11
, 184 subjects developed symptomatic neuropathy ( incident neuropathy ) , 948 remained neuropathy free , 67 continued with neuropathy , and 64 became neuropathy free .
the mean ( sd ) age was 73.5 ( 2.9 ) , and 51.7% were female .
participants with normoglycemia accounted for 49.1% of the population , prediabetes 29.9% , and diabetes 21.0% .
demographics of the health abc cohort at year 1 stratified by glycemic status dbp , diastolic blood pressure ; sbp , systolic blood pressure .
the prevalence of symptomatic dsp was significantly higher in participants with higher number of mets components ( p = 0.03 ) , controlling for glycemic status ( fig .
the dsp prevalence was 8.5% in those with no mets components ( excluding hyperglycemia ) compared with 12.9% in those with four mets components . in those with normoglycemia , the dsp prevalence was 8.6% in those with no mets components compared with 11.4% in those with four mets components . in those with prediabetes , the dsp prevalence was 5.6% in those with no additional mets components compared with 10.6% in those with four additional mets components .
in contrast , the dsp prevalence in participants with diabetes was similar between those with no additional mets components ( 16.7% ) and those with four additional mets components ( 17.1% ) .
dsp was defined as those with neuropathic symptoms ( questionnaire ) plus at least 1 of 3 confirmatory tests ( heavy monofilament , peroneal cv , or vibration threshold ) in year 4 of the study .
glycemic status was determined by the glucose tolerance test according to the expert committee on the diagnosis and classification of diabetes mellitus in year 1 .
mets components were defined using the updated national cholesterol education program / adult treatment panel iii definition . in a multivariable logistic regression model investigating the individual mets components , diabetes ( odds ratio [ or ] 1.65 [ 95% ci 1.182.31 ] ) , height ( or 1.39 [ 95% ci 1.241.54 ] , unit = 5 cm ) , and male sex ( or 0.59 [ 95% ci 0.390.92 ] ) were significantly associated with the dsp primary outcome ( table 2 ) .
prediabetes , waist circumference , systolic blood pressure , triglycerides , and hdl were not significantly associated with dsp . in a separate multivariable logistic regression model investigating the association of the number of mets components in addition to glycemic status ,
the number of mets components ( or 1.20 [ 95% ci 1.061.37 ] ) was significantly associated with symptomatic dsp .
multivariable logistic regression evaluating the association of mets components ( year 1 ) and neuropathy ( year 4 ) ( cross - sectional ) data are or ( 95% ci ) .
sbp , systolic blood pressure . in investigation of the association of mets components and the 6 secondary neuropathy outcomes ,
increasing age and height were significantly associated with all neuropathy outcome measures with the exception of neuropathic symptoms . increasing
while systolic blood pressure and triglycerides were significantly associated with one and two neuropathy outcomes , respectively , the association was in the opposite direction of what was expected ( lower blood pressure and lower triglycerides ) .
multivariable linear regression evaluating the association of mets components ( year 1 ) and continuous outcomes of neuropathy ( year 4 ) ( cross - sectional ) sbp , systolic blood pressure . in a multivariable logistic regression model evaluating the longitudinal change of mets components ,
only the baseline hemoglobin a1c ( year 4 or 1.42 [ 95% ci 1.151.75 ] ) and height ( or 1.26 [ 95% ci 1.091.46 ] ) were significantly associated with incident symptomatic neuropathy .
baseline weight ( or 0.99 [ 95% ci 0.921.07 ] ) , systolic blood pressure ( or 1.06 [ 95% ci 0.971.17 ] ) , triglycerides ( or 0.99 [ 95% ci 0.841.16 ] ) , and hdl ( or 0.94 [ 95% ci 0.811.09 ] ) were not significantly associated with incident neuropathy .
similarly , the slopes of the mets components ( hemoglobin a1c or 1.91 [ 95% ci 0.2812.95 ] , weight or 0.71 [ 95% ci 0.202.55 ] , systolic blood pressure or 1.54 [ 95% ci 0.623.86 ] , triglyceride or 0.79 [ 95% ci 0.134.85 ] , and hdl or 0.87 [ 95% ci 0.135.88 ] ) were not significantly associated with incident neuropathy .
in a multivariable logistic regression model investigating the individual mets components , diabetes ( odds ratio [ or ] 1.65 [ 95% ci 1.182.31 ] ) , height ( or 1.39 [ 95% ci 1.241.54 ] , unit = 5 cm ) , and male sex ( or 0.59 [ 95% ci 0.390.92 ] ) were significantly associated with the dsp primary outcome ( table 2 ) .
prediabetes , waist circumference , systolic blood pressure , triglycerides , and hdl were not significantly associated with dsp . in a separate multivariable logistic regression model investigating the association of the number of mets components in addition to glycemic status ,
the number of mets components ( or 1.20 [ 95% ci 1.061.37 ] ) was significantly associated with symptomatic dsp .
multivariable logistic regression evaluating the association of mets components ( year 1 ) and neuropathy ( year 4 ) ( cross - sectional ) data are or ( 95% ci ) .
sbp , systolic blood pressure . in investigation of the association of mets components and the 6 secondary neuropathy outcomes ,
increasing age and height were significantly associated with all neuropathy outcome measures with the exception of neuropathic symptoms . increasing
while systolic blood pressure and triglycerides were significantly associated with one and two neuropathy outcomes , respectively , the association was in the opposite direction of what was expected ( lower blood pressure and lower triglycerides ) .
multivariable linear regression evaluating the association of mets components ( year 1 ) and continuous outcomes of neuropathy ( year 4 ) ( cross - sectional ) sbp , systolic blood pressure .
in a multivariable logistic regression model evaluating the longitudinal change of mets components , only the baseline hemoglobin a1c ( year 4 or 1.42 [ 95% ci 1.151.75 ] ) and height ( or 1.26 [ 95% ci 1.091.46 ] ) were significantly associated with incident symptomatic neuropathy .
baseline weight ( or 0.99 [ 95% ci 0.921.07 ] ) , systolic blood pressure ( or 1.06 [ 95% ci 0.971.17 ] ) , triglycerides ( or 0.99 [ 95% ci 0.841.16 ] ) , and hdl ( or 0.94 [ 95% ci 0.811.09 ] ) were not significantly associated with incident neuropathy .
similarly , the slopes of the mets components ( hemoglobin a1c or 1.91 [ 95% ci 0.2812.95 ] , weight or 0.71 [ 95% ci 0.202.55 ] , systolic blood pressure or 1.54 [ 95% ci 0.623.86 ] , triglyceride or 0.79 [ 95% ci 0.134.85 ] , and hdl or 0.87 [ 95% ci 0.135.88 ] ) were not significantly associated with incident neuropathy .
in our large cohort of older black and white men and women , 21.0% of the population had diabetes and 52.8% met criteria for mets at baseline .
these numbers are comparable with those in previous studies using national health and nutrition examination survey ( nhanes ) data , which have estimated a diabetes prevalence of 20% in those over 65 years of age and a mets prevalence of 4758% in those over 70 years of age ( 26,27 ) . in our well - functioning older cohort ,
a previous nhanes study revealed similar results with a symptomatic dsp prevalence of 3.1% in those over 40 years of age , which was three times higher in those age 7079 years ( 2 ) . a study in italy in those over the age of 55 years revealed a symptomatic dsp prevalence of 78% with an even higher prevalence in those > 75 years ( 1 ) .
similarly , others have estimated a clinical neuropathy prevalence of 69% in those > 7079 years old ( 28 ) . in concert with previous studies ,
we demonstrate that the prevalence of diabetes , mets , and dsp is incredibly high in even a well - functioning older population . as a result
, any causal link between mets and neuropathy , regardless of diabetes status , would have potential therapeutic implications for a large proportion of older adults .
furthermore , previous studies have demonstrated that the peripheral nerve measures used in this study are associated with important functional outcomes in older populations including mobility , quadriceps strength , and other physical performance measures ( 2931 ) .
therefore , therapeutic interventions to prevent and/or ameliorate dsp in this population may have a clinically meaningful effect .
we found a statistically significant association between the number of mets components and symptomatic dsp when adjusting for glycemic status in two separate analyses .
in fact , the dsp prevalence increased from 8.5 to 12.9% when we compared subjects with no mets components , excluding hyperglycemia , with those with all four components .
the implication is that mets components may be important risk factors for the development of dsp independent of glycemic status .
the magnitude of the effect of additional mets components on dsp prevalence ( 1.1% difference per component ) is not as high as the effect of diabetes ( 8.6% ) .
however , given the high prevalence of mets components individually and in combination , this effect may be clinically important
. our results are comparable with those of the recent prospective metabolism and islet cell evaluation ( promise ) study in patients at high risk of diabetes that demonstrated that the vibration threshold increased as the number of mets components increased ( 32 ) .
however , in contrast to our analysis , this association did not persist after adjustment for participant demographics .
interestingly , we found that the rise in dsp prevalence with additional mets components was most evident in those with normoglycemia and prediabetes .
the number of mets components did not affect the dsp prevalence in those with diabetes . of note
future studies investigating the effect of treatment of mets components on the prevention or treatment of dsp may need to target the normoglycemic populations and populations with prediabetes .
the prevalence of symptomatic dsp in those with prediabetes ( 9.3% ) was comparable with the prevalence in those with normoglycemia ( 9.7% ) .
furthermore , prediabetes was not associated with any of our neuropathic outcome measures in multivariable regression models .
these observations add to other conflicting studies on the importance of prediabetes as a cause of dsp .
the monica / kora ( multinational monitoring of trends and determinants in cardiovascular disease / cooperative health research in the region of augsburg ) , san luis valley , and promise ( all with dsp defined by neurologic examination ) studies all revealed an increased prevalence of dsp in those with impaired glucose tolerance compared with normoglycemia ( 16,23,32 ) .
however , investigators using nhanes data reported a dsp prevalence ( based on monofilament testing ) comparable with that in our population with no significant difference between those with normoglycemia ( 10.5% ) and those with impaired fasting glucose ( 11.9% ) ( 22 ) .
all of these studies used different definitions of dsp , were conducted in different populations , and included subjects of different ages .
one possible explanation for the discrepant results is that the effect of prediabetes in older adults is less than in younger adults .
alternatively , the neuropathy definition used in this study was insufficient to capture small fiber predominant neuropathy , which is typically seen in those with either prediabetes and/or mets ( 33 ) .
another possibility is that prediabetes is not as important as the other mets components that tend to cluster with this condition .
future studies are needed to further understand the role of prediabetes in dsp . a clinical trial investigating
the effects of the treatment of prediabetes on neuropathy outcomes may be the only way to definitely address this question .
diabetes was the only mets component that was significantly associated with the primary dsp outcome in both cross - sectional and longitudinal analyses .
however , waist circumference ( four of six ) and hdl ( two of six ) were associated with some of the secondary neuropathy outcome measures .
one potential reason for the lack of associations between mets components and dsp is that we did not have enough power to detect the associations , which likely have smaller effect sizes .
the power of our secondary outcomes may have been more robust because most had the potential to capture asymptomatic dsp as well as symptomatic dsp .
furthermore , we did not have adequate power to investigate interactions between glycemic status and the other mets components .
interactions are likely important , as the prevalence of dsp only increased as the number of mets components increased in those with normoglycemia and prediabetes .
another possibility is that the main drivers of dsp are not the mets components themselves but , rather , other factors that are associated with them .
for example , perhaps abdominal obesity is not one of the main drivers of dsp and , instead , adipose tissue  derived inflammatory markers , such as interleukin-6 levels or adiponectin - to - leptin ratios , are the true mediators ( 34 ) .
further studies with larger samples sizes and more precise mets and neuropathy measurements are needed to further delineate the role of mets components in the development of dsp .
limitations include the lack of a neurologic examination to determine neuropathy status and neuropathic symptoms based on a questionnaire
. however , we were able to combine questionnaire items pertaining to neuropathic symptoms in the feet with well - validated neuropathy confirmatory studies .
our sample size was not adequate to address small effect sizes or interaction between mets components and glycemic status .
similarly , the sample size in the longitudinal analysis was smaller than the cross - sectional analysis
. nevertheless , our study is one of the largest to date to investigate the association between mets components and dsp .
our analysis was confined to those well - functioning 7079 year olds at the start of the study . how our results apply to other populations
mets components are significantly associated with dsp independent of glycemic status , although the effect is less than that of the presence of diabetes .
the precise components , or combinations of components , that drive the development of dsp remain unclear .
larger studies with more precise metabolic measures are needed to guide the development of future intervention studies .
given the high prevalence of both the mets and dsp , particularly in older adults , even small causal effects have the potential for a large impact on population health .
 objectiveprevious studies demonstrate that the metabolic syndrome is associated with distal symmetric polyneuropathy ( dsp ) . 
 we aimed to determine the magnitude of this effect and the precise components involved.research design and methodswe determined the symptomatic dsp prevalence in the health , aging , and body composition ( health abc ) study ( prospective cohort study , with subjects aged 7079 years at baseline ) , stratified by glycemic status ( glucose tolerance test ) and the number of additional metabolic syndrome components ( updated national cholesterol education program / adult treatment panel iii definition ) . 
 dsp was defined as neuropathic symptoms ( questionnaire ) plus at least one of three confirmatory tests ( heavy monofilament , peroneal conduction velocity , and vibration threshold ) . 
 multivariable logistic and linear regression evaluated the association of metabolic syndrome components with dsp in cross - sectional and longitudinal analyses.resultsof 2,382 participants with neuropathy measures ( mean age 73.5  2.9 years , 38.2% black , 51.7% women ) , 21.0% had diabetes , 29.9% prediabetes , 52.8% metabolic syndrome , and 11.1% dsp . 
 stratified by glycemic status , dsp prevalence increased as the number of metabolic syndrome components increased ( p = 0.03 ) . 
 diabetes ( cross - sectional model , odds ratio [ or ] 1.65 [ 95% ci 1.182.31 ] ) and baseline hemoglobin a1c ( longitudinal model , or 1.42 [ 95% ci 1.151.75 ] ) were the only metabolic syndrome measures significantly associated with dsp . 
 waist circumference and hdl were significantly associated with multiple secondary neuropathy outcomes.conclusionsindependent of glycemic status , symptomatic dsp is more common in those with additional metabolic syndrome components . 
 however , the issue of which metabolic syndrome components drive this association , in addition to hyperglycemia , remains unclear . 
 larger waist circumference and low hdl may be associated with dsp , but larger studies with more precise metabolic measures are needed . 
Introduction
Research Design and Methods
Population
MetS Components
DSP Definition
Statistical Analysis
Cross-sectional Analyses
Longitudinal Analysis
Results
Cross-sectional Analyses
Longitudinal Analysis
Conclusions