understanding how the vertebrate nervous system develops from the embryonic ectoderm has been one of the most intensely investigated developmental processes .
the process of  neural induction  was discovered by hans spemann and hilde mangold ; when dorsal mesoderm was transplanted adjacent to ventral ectoderm that normally would give rise to skin instead it formed a neural tube ( spemann and mangold , 1923 , reprinted 2001 ) .
this result was interpreted to mean that dorsal mesoderm releases a signaling factor that converts the adjacent ectoderm into neural tissue . while the identity of this signal was sought for decades , only in recent years
have we come to appreciate the signaling and transcription factors that mediate neural induction and control neural differentiation .
recently it has been possible to place these factors in a gene regulatory network that controls the progression of cells from newly induced neural precursors , to proliferative , multipotent neural plate stem cells , to specified neural progenitors .
these early steps of neural development are critical for producing a pool of multipotent cells that later give rise to the multitude of neurons and glia in the central nervous system that have diverse and distinct functions , and are selectively affected in a number of human neurodegenerative diseases . research to produce a population of cells in vitro that recapitulate the differentiated progeny seen in the intact nervous system has defined a  neural stem cell  ( nsc ) , which is a self - renewing cell whose progeny can produce neurons , oligodendrocytes and astrocytes ( fujita , 2003 ) . in the embryonic neural tube these cells reside in the ventricular zone ( or subventricular zone in the developing cerebral cortex ) , and in both the embryo and in nsc cultures , these cells are activated to produce new progeny by the integration of a number of signaling pathways , such as sonic hedgehog , bmp , wnt/catenin , notch , and fgf ( fig . 1 ; brault et al . , 2001 ;
, 2008 ; mizutani et al . , 2007 ; ohtsuka et al . , 2001 ; palma and ruiz i altaba , 2004 ; zechner et al . , 2003 )
the self - renewal ability of these cells is regulated by numerous transcription factors , including he s , sox , bmi1 , tlx , and gli proteins ( fig .
1 ; ahmed et al . , 2009 ; bylund et al . , 2003 ;
, 2004 ; ishibashi et al . , 1995 ; miyagi et al . , 2008 ;
the recognition of this cell has inspired numerous researchers to develop new culturing approaches to obtain specific kinds of neurons or glia for therapeutic purposes , such as striatal neurons for huntington s disease and dopamine neurons for parkinson s disease . while there has been much success ( e.g. , reviewed in chan et al .
, 2014 ; ross and akimov , 2014 ) , the potential presence of pluripotent stem cells in these cultures remains a concern because they might cause tumors if transplanted into patients .
therefore , in this review we will focus on what we know about the functions of the signaling and transcriptional factors that control the earliest steps in neural development in the vertebrate embryo .
these are the steps that control commitment to a neural fate , proliferation of immature cells , and initiation of differentiation .
if we can determine whether the molecular interactions that occur in the embryo to regulate how cells initially become committed to a neural fate are similarly employed when embryonic stem cells ( escs ) and induced pluripotent stem cells ( ipscs ) are differentiated into nscs , we will be able to eliminate tumorigenic cells .
therefore , we will relate the findings from embryos to studies of cultured mammalian stem cells in expectation that the knowledge gained from the embryo will significantly enhance in vitro stem cell approaches and thereby yield transplantable neural precursors for regenerative applications .
the vertebrate neural ectoderm forms on the dorsal side of the embryo in response to signals from the adjacent dorsal mesoderm , which is called the  organizer  in frog and fish and the  node  in chick and mouse ( reviewed in de robertis and kuroda , 2004 ; itoh and sokol , 2007 ; levine and brivanlou , 2007 ; moody et al . , 2013 ; rogers et al . ,
small diffusible proteins that are secreted from the organizer bind to either bmp or wnt ligands and prevent them from activating their receptors in the adjacent ectoderm .
embryonic ectodermal cells become epidermis ( skin ) in the presence of bmps and wnts and become neural ectodermal ( ne ) precursors in their absence ( fig .
1 ) . the newly induced ne precursors express a large number of neural transcription factors ( ntfs ) , including members of the fox , sox , zic and irx families , that are co - expressed in broad overlapping domains .
some of these factors regulate the competence of the ne precursors to respond to neural inducing signals and cause the newly induced neural cells to become refractory to bmp and wnt signals ( moody et al . , 2013 ; rogers et al .
fgf signaling is another important pathway that facilitates neural induction ( fig . 1 ; streit et al . , 1998 ; 2000 ;
one of the downstream effectors of the fgf neural promoting activity is a protein called churchill ( churc ) , which up - regulates early neural genes and down - regulates early mesodermal genes ( sheng et al . , 2003 ) .
its activity is mediated , at least in part , by a smad - interacting protein called sip1 ( verschueren et al . , 1999 ) .
sip1 is expressed in the dorsal ectoderm at the time that neural induction is occurring , converts nave ectoderm to neural ectoderm , represses mesoderm genes and inhibits bmp signaling ( eisaki et al .
the fgf - mediated switch from mesodermal to neural fates also is regulated by a pluripotency transcription factor , pou91/oct4 , upstream of churc and sip1(snir et al . , 2006 ) .
thus , these factors appear to be activated at the time cells are switching off a pluripotency program . adding neural inducing factors to esc or ipsc cultures also directs these cells to become ne precursors ( also called primitive neural stem cells ) ( devine et al . , 2011 ; tropepe et al . ,
some of the cells in esc cultures secrete bmps and wnts , and maintaining ne precursors depends on inhibiting these signals ( tropepe et al . , 2001 ) , just as in the embryo . maintaining the expression of sox1 in human esc and ipsc cultures
also requires continuous down - regulation of bmp signaling ( chaddah et al . , 2012 ; neely et al . , 2012 ) .
however , unlike in the embryo , fgf signaling inhibits human escs from forming neural tissues , at least under some culture conditions ( greber et al . , 2011 ) .
it is not clear whether this means that fgf signals have different effects in embryos versus escs , or whether fgf signaling acts to promote mesoderm formation under these culture conditions
. it will be very informative to more precisely determine the roles of fgf signaling in neural induction of cultured stem cells .
to our knowledge , no studies have been published regarding churc function in stem cell cultures .
but , sip1 plays a key role in the decision between neural ectoderm and mesendoderm in human escs and in mouse epiblast stem cells ( chng et al . , 2010 ) .
thus , the evidence so far suggests that the induction of the ne precursor state in esc and ipsc cultures relies upon some of the same signaling factors as in the embryo .
further study of the timing and effectiveness of these factors in both embryos and stem cell cultures may reveal how to drive cells towards a stabilized neural fate and prevent the formation of unwanted lineages .
once the neural ectoderm is induced and the ntfs are expressed , the tissue continues to be exposed to both bmp and wnt signals from the surrounding mesoderm and ectoderm .
it has been proposed that some of the earliest expressed ntfs somehow stabilize the neural fate program , and thereby prevent cells from reverting to a non - neural fate ( sasai , 1998 ) . since several of the ntf genes
can be directly repressed by bmp - regulated transcription factors ( rogers et al . , 2008
2006 ) , stabilizing a cell s commitment to a neural fate is important as the signaling environment changes in the embryo .
one such factor is zic1 , which causes the embryonic ectoderm to be more sensitive to neural inductive signals ( kuo et al . , 1998 ) .
to date , the mechanism by which this occurs has not been defined , but perhaps zic1 attenuates some aspect of bmp or wnt signaling .
gmnn and irx1/xiro1 reduce bmp4 mrna levels when ectopically expressed in epidermal domains ( glavic et al .
, 2001 ; gomez - skarmeta et al . , 2001 ; kroll et al . ,
sox3 down - regulates the expression of the bmp target , vent2 , which is required for epidermis formation ( rogers et al . , 2008 ; 2009b ) .
foxd4 down - regulates the expression of several genes in the bmp pathway , including their epidermal gene targets , and also reduces the nuclear localization of the phosphorylated smads that are the effectors of bmp signaling ( yan et al . , 2009 ; 2010 ) .
sox11 interacts with the map kinase nlk to antagonize wnt signaling by phosphorylating the tcf/catenin complex ( hyodo - miura et al . , 2002 ) .
ectopic expression of foxd4 mrna in the epidermal lineage , which is a field of high bmp / wnt expression , induces several other ntfs in presumptive epidermal cells , indirectly indicating that the bmp and/or wnt pathways have been repressed ( yan et al . , 2009 ) .
in fact , we recently found that mouse foxd4 has the same effects in xenopus embryos , indicating conservation of this protein s function ( unpublished ) .
these studies in embryos demonstrate that the combined anti - bmp and anti - wnt activities of several of the earliest expressed ntfs maintain a permissive neural ectoderm environment by dampening the effects of inhibitory bmp and wnt signals that persist in the embryo after neural induction .
maintenance of a bmp / wnt - free environment effectively stabilizes the neural fate of the ne precursors , which allows them to transition into neural plate stem cells and prevents them from converting back to a non - neural state . to our knowledge
, the roles of these early ntfs in stabilizing neural fates in mammalian esc cultures have not been explored .
we hypothesize that ntfs have a role because maintaining ne precursors depends on inhibiting bmp and wnt signals ( tropepe et al . , 2001 ) , and maintaining the expression of sox1 , another ntf , in human esc and ipsc cultures requires continuous down - regulation of bmp signaling ( chaddah et al . , 2012 ; neely et al . , 2012 ) .
thus , the maintenance of the ne precursor state in esc and ipsc cultures may rely upon some of the same signaling and transcription factors as in the embryo .
once the neural ectoderm has been induced , the cells become highly proliferative and take on a columnar shape , thus forming the neural plate , a morphologically distinct domain that will give rise to the central nervous system . numerous experiments in embryos
show that when the level of each ntf is experimentally increased in ne precursors the neural plate is expanded .
this phenotype may occur because the ntfs have broadened the domain in which bmp signaling is repressed , as described above
. however , neural plate expansion also appears to result from the ability of some ntfs to promote the proliferation of neural plate stem cells , and/or delay their differentiation into neural progenitors .
for example , in xenopus embryos , gmnn maintains ne precursors in a proliferative state by modulating interactions between the swi / snf complex and the bhlh transcription factors that promote neural differentiation ( seo and kroll , 2006 ; seo et al . , 2005 ) .
foxd4 also increases the number of proliferating cells , expands markers of immature neural ectoderm , and down - regulates bhlh neural differentiation genes ( moody et al . , 2013 ; sullivan et al . , 2001
interestingly , gmnn and foxd4 are down - regulated as neural plate stem cells exit the cell cycle and differentiate into neural progenitors ( kroll et al .
, 1998 ; sullivan et al . , 2001 ) , indicating that their activities must be suppressed for neural differentiation to proceed .
zic2 also represses bhlh neural differentiation genes and counteracts the formation of extra neurons when bhlh genes are ectopically expressed in the epidermis ( brewster et al . , 1998 ) .
these studies indicate that gmnn , foxd4 and zic2 cause neural plate expansion by promoting ne precursor and neural stem cell proliferation and delaying the bhlh - mediated establishment of differentiating neural progenitors .
other ntfs appear to promote the transition of neural plate stem cells to neural progenitor cells , a process that requires cells to exit the cell cycle and initiate bhlh neural differentiation gene expression ( imayoshi and kageyama , 2014 ) . in several animals , soxb1 family members ( sox1 , sox2 , sox3 ) maintain neural stem populations and must be down - regulated for neural progenitor differentiation to proceed ( bylund et al .
when expressed at high levels , each maintains neural stem cells in a proliferative state upstream of neuronal differentiation genes ( bani - yaghoub et al .
, 2006 ; ellis et al . , 2004 ; graham et al . , 2003 ; li et al . , 1998;wang et al . , 2006 ; wegner and stolt , 2005 ; zappone et al
. likewise , sox11 , a member of the soxc subfamily , is up - regulated as neural stem cells transition to neural progenitor cells , and later maintains neuronal progenitors ( bergsland et al .
thus , together these sox genes may function downstream of foxd4 , gmnn and zic2 to promote the initial step from neural plate stem cell to neural progenitor cell .
several studies show that other zic genes and the irx genes function in neural progenitors downstream of the soxb1 genes to promote the onset of bhlh neural differentiation gene expression .
zic1 is required for the expression of soxd ( a member of the soxg group ) , which causes ectopic neural masses that express bhlh neural differentiation genes ( mizuseki et al . , 1998b ) , and zic1 and zic3 promote the expansion of neural progenitors in the spinal cord and forebrain ( aruga et al . , 2002 ; nakata et al . , 1997 ; 1998 ) .
in drosophila , iroquois genes are required for the activation of the proneural bhlh genes ( gomez - skarmeta et al . , 1996 ) , and in xenopus
the homologous irx / xiro genes are expressed just prior to the earliest expressed bhlh neural differentiation genes(bellefroid et al . , 1998 ) . while irx genes promote the onset of neural differentiation ( bellefroid et al . , 1998 ;
1998 ) , they also suppress terminal differentiation into neurons ( de la calle - mustienes et al . , 2002 ) .
these studies demonstrate that the ntfs have important roles in maintaining a ne precursor state , regulating the size of the neural plate stem cell population and controlling the onset of differentiation of the neural progenitor cells .
to date , most emphasis has been put on forcing stem cells to a neural fate and then differentiating them into specific neuronal and glial types ( chan et al . , 2014 ; ross and akimov , 2014 ; wichterle et al . , 2002 ) .
however , a common problem with these protocols is the difficulty in maintaining neural precursors in an immature , undifferentiated state . for many therapeutic applications this would be advantageous because it would expand neural precursor cells without the spontaneous and premature differentiation that commonly occurs in these cultures . in the embryo ,
next , as the neural plates forms , these cells transition into neural plate stem cells which subsequently differentiate into regionally - specified neural progenitor cells . whereas in the embryo , proliferative ,
undifferentiated neural precursors can be maintained , it is rare to see this kind of cell in esc cultures .
however , similar primitive neural precursors do form in mouse esc cultures when neural inductive signaling is maintained either by adding anti - bmp factors or growing cells at a low enough density to dilute endogenously produced bmps ( tropepe et al .
, 2001 ) . the molecular mechanisms by which this primitive state is acquired and maintained , and by which these cells transition to the better characterized neural stem cell state will be important to elucidate in light of the potential to eliminate tumor - producing pluripotent cells .
the studies summarized above took a gene - by - gene approach to understand the functions of each ntf during the progressive transition from neural inductive signaling to neural progenitor differentiation . based on their sequential and overlapping activities it seems likely that the ntfs coordinately function in a gene regulatory network ( fig .
2 ) . an initial set of ntfs maintains cells in a stable , immature ne precursor state that proliferates to form an appropriately sized neural plate .
these are then down - regulated and a different set of ntfs is activated to promote the formation of the neural stem cell constituents of the neural plate .
finally , neural stem cell ntfs are down - regulated and a third set of ntfs is activated that initiate neural differentiation .
our understanding of the functional and transcriptional relationships between these proteins is woefully incomplete , yet this information is fundamental for understanding how the balance between neural precursor , neural stem and neural progenitors is molecularly regulated .
developmental events often are controlled by regulatory networks of transcription factors that control temporal and region - specific gene expression ( levine and davidson , 2005 ) .
therefore , we sought to place these ntfs in a network to determine their distinct roles in the progression from ne precursors to differentiating neural progenitors by analyzing the epistatic position of foxd4 amongst these other ntfs . using protein knock - down approaches we found that foxd4 is required for the expression of each of the other ntfs(yan et al . , 2009 ) , thus placing it upstream in the network ( fig .
2 ) . using gain - of - function approaches , we found that elevated foxd4 levels in ne precursors up - regulated gmnn and zic2 , delayed the expression of sox2 , sox3 and sox11 , which resulted in an expanded neural plate ; and down - regulated the zic and irx genes that promote neural progenitor differentiation .
we also found that foxd4 expression is down - regulated at early neural plate stages , suggesting that its repression is required for ne precursors to progress to neural plate stem cells and to neural progenitors .
additional experiments demonstrated that gmnn , zic2 and sox11 together can mediate the foxd4 effects on the remaining ntfs(yan et al . , 2009 ) .
from these results we can construct a gene regulatory network in which foxd4 is a critical upstream component .
it directly activates a transcriptional triad consisting of gmnn , zic2 and sox11 , which in turn regulates the more down - stream components that promote the transition to neural stem and neural progenitor cells ( fig .
, structure - function analyses of the foxd4 protein show that it has separate domains in the n - and c - terminal regions that account for its ability to both activate or repress targets ( klein et al .
these findings illustrate how a single transcription factor can regulate the transition of immature , ne precursors to neurally - committed stem cells , and then to neural progenitors that are beginning to differentiate .
the experiments described above demonstrate that foxd4 is a critical element in the gene regulatory network that regulates the balance between ne precursors , neural plate stem cells and regionally specified neural progenitor cells . elucidating how these different ntf genes interact to regulate this balance between expanding precursor populations and initiating differentiation should ultimately prove useful for preventing the formation of mesodermal and endodermal cells in neuronal culture protocols , expanding the number of neural precursors available and preventing their premature differentiation .
one would predict from the experiments performed in embryos that during the differentiation of escs into nscs foxd4 , gmnn , zic2 and sox11 would act early to promote immature , proliferative ne precursors and impede neural differentiation ( fig .
our preliminary work analyzing the role of foxd4 in embryoid bodies derived from mouse escs and differentiated along a neural lineage indicates that this protein is key to the transition between pluripotency and neurally committed cells , just as in the embryo ( moody et al . , 2013 , and unpublished observations ) . in the embryo
, gmnn promotes an uncommitted state by promoting polycomb mediated repressive histone marks at differentiation - promoting genes ( lim et al . , 2011 ) .
it also prevents premature neurogenesis by antagonizing the interaction between brg and bhlh neural differentiation factors ( seo et al . , 2005 ) .
just like in the embryo , in mouse escs gmnn is required for the acquisition of a neural fate , and can promote the formation of neural precursor cells by maintaining the chromatin in a hyper - acetylated state and in an open conformation that allows other ntfs access to their binding sites on the dna ( yellajoshyula et al . , 2011 ) .
subsequent work showed that gmnn promotes both activating and repressive histone modifications at neural differentiation genes , suggesting that gmnn makes the chromatin accessible to both factors that promote ne precursors as well as to factors that will subsequently activate neural differentiation genes ( yellajoshyula et al . , 2012 ) .
these results explain why gmnn - deficient neural progenitors in the mouse cortex are defective in cell proliferation and differentiation ( spella et al . , 2011 ) , whereas gmnn - deficient nscs in neurospheres can still divide and differentiate into neurons and glia ( schultz et al . , 2011 ) . whereas the role of zic2 has not been examined in esc culture ,
soxb1 family proteins ( sox1 , sox2 , sox3 ) are expressed throughout mouse and chick embryo ne precursors and neural plate stem cells , and when expressed at high levels they prevent cells from differentiating into neurons ( uwanogho et al . , 1995 ; wegner , 1999 ) .
in addition , sox2 has an earlier function in escs : maintenance of pluripotency(avilion et al . , 2003 ) .
sox11 is expressed through - out ne precursors and neural plate stem cells , overlapping completely with sox2 and sox3 expression in xenopus ( hyodo - miura et al . , 2002 ) , whereas in chick and mouse embryos , it is expressed later in neural progenitors and differentiating neurons , and does not overlap with sox2 or sox3 expression in the neural tube ( uwanogho et al . , 1995 ;
wegner , 1999 ) ; in mouse it also is expressed in several other tissues to support progenitor cell survival ( bhattaram et al . , 2010 ) .
a recent study showed that sox2 , sox3 and sox11 bind to the promoters / enhancers of the same neural genes expressed in mouse nscs , neural progenitors and differentiating neurons in a defined temporal sequence ( bergsland et al . , 2011 ) .
in escs , sox2 binds to nsc genes to mark sites for later sox3 binding .
subsequently in nscs , sox3 binds to neuronal differentiation genes to mark sites for later sox11 binding . in each case , sox protein binding results in biva - lent chromatin marks , indicating that part of their effect is making the appropriate neural genes accessible to other transcriptional factors that are expressed later .
there is a large literature that describes the ntfs that are involved in neural progenitor cell ( npc ) formation that has been extensively reviewed elsewhere ( florio and huttner , 2014 ; imayoshi and kageyama , 2014 ) . as mentioned above ,
the bhlh factors , particularly neurog and neurod are critical for npcs to exit the cell cycle and activate neuron or glial specific genes .
in addition , pattern formation genes are important in providing npcs with their spatial address within the neural tube . depending upon
whether the npc is located in the anterior or posterior , dorsal or ventral parts of the neural tube , the expression of patterning genes will impact whether it differentiates into the forebrain versus spinal cord , and sensory versus motor neuron .
once the neural ectoderm is induced and the ntfs are expressed , the tissue continues to be exposed to both bmp and wnt signals from the surrounding mesoderm and ectoderm .
it has been proposed that some of the earliest expressed ntfs somehow stabilize the neural fate program , and thereby prevent cells from reverting to a non - neural fate ( sasai , 1998 ) . since several of the ntf genes
can be directly repressed by bmp - regulated transcription factors ( rogers et al . , 2008 ; taylor et al . ,
2006 ) , stabilizing a cell s commitment to a neural fate is important as the signaling environment changes in the embryo .
one such factor is zic1 , which causes the embryonic ectoderm to be more sensitive to neural inductive signals ( kuo et al . , 1998 ) . to date , the mechanism by which this occurs has not been defined , but perhaps zic1 attenuates some aspect of bmp or wnt signaling .
gmnn and irx1/xiro1 reduce bmp4 mrna levels when ectopically expressed in epidermal domains ( glavic et al .
sox3 down - regulates the expression of the bmp target , vent2 , which is required for epidermis formation ( rogers et al . , 2008 ; 2009b ) .
foxd4 down - regulates the expression of several genes in the bmp pathway , including their epidermal gene targets , and also reduces the nuclear localization of the phosphorylated smads that are the effectors of bmp signaling ( yan et al .
sox11 interacts with the map kinase nlk to antagonize wnt signaling by phosphorylating the tcf/catenin complex ( hyodo - miura et al . , 2002 ) .
ectopic expression of foxd4 mrna in the epidermal lineage , which is a field of high bmp / wnt expression , induces several other ntfs in presumptive epidermal cells , indirectly indicating that the bmp and/or wnt pathways have been repressed ( yan et al . , 2009 ) .
in fact , we recently found that mouse foxd4 has the same effects in xenopus embryos , indicating conservation of this protein s function ( unpublished ) .
these studies in embryos demonstrate that the combined anti - bmp and anti - wnt activities of several of the earliest expressed ntfs maintain a permissive neural ectoderm environment by dampening the effects of inhibitory bmp and wnt signals that persist in the embryo after neural induction .
maintenance of a bmp / wnt - free environment effectively stabilizes the neural fate of the ne precursors , which allows them to transition into neural plate stem cells and prevents them from converting back to a non - neural state . to our knowledge
, the roles of these early ntfs in stabilizing neural fates in mammalian esc cultures have not been explored .
we hypothesize that ntfs have a role because maintaining ne precursors depends on inhibiting bmp and wnt signals ( tropepe et al . , 2001 ) , and maintaining the expression of sox1 , another ntf , in human esc and ipsc cultures requires continuous down - regulation of bmp signaling ( chaddah et al . , 2012 ; neely et al .
thus , the maintenance of the ne precursor state in esc and ipsc cultures may rely upon some of the same signaling and transcription factors as in the embryo .
once the neural ectoderm has been induced , the cells become highly proliferative and take on a columnar shape , thus forming the neural plate , a morphologically distinct domain that will give rise to the central nervous system . numerous experiments in embryos
show that when the level of each ntf is experimentally increased in ne precursors the neural plate is expanded .
this phenotype may occur because the ntfs have broadened the domain in which bmp signaling is repressed , as described above
. however , neural plate expansion also appears to result from the ability of some ntfs to promote the proliferation of neural plate stem cells , and/or delay their differentiation into neural progenitors .
for example , in xenopus embryos , gmnn maintains ne precursors in a proliferative state by modulating interactions between the swi / snf complex and the bhlh transcription factors that promote neural differentiation ( seo and kroll , 2006 ; seo et al . , 2005 ) .
foxd4 also increases the number of proliferating cells , expands markers of immature neural ectoderm , and down - regulates bhlh neural differentiation genes ( moody et al . , 2013 ; sullivan et al . , 2001
interestingly , gmnn and foxd4 are down - regulated as neural plate stem cells exit the cell cycle and differentiate into neural progenitors ( kroll et al .
, 1998 ; sullivan et al . , 2001 ) , indicating that their activities must be suppressed for neural differentiation to proceed .
zic2 also represses bhlh neural differentiation genes and counteracts the formation of extra neurons when bhlh genes are ectopically expressed in the epidermis ( brewster et al . ,
these studies indicate that gmnn , foxd4 and zic2 cause neural plate expansion by promoting ne precursor and neural stem cell proliferation and delaying the bhlh - mediated establishment of differentiating neural progenitors .
other ntfs appear to promote the transition of neural plate stem cells to neural progenitor cells , a process that requires cells to exit the cell cycle and initiate bhlh neural differentiation gene expression ( imayoshi and kageyama , 2014 ) .
in several animals , soxb1 family members ( sox1 , sox2 , sox3 ) maintain neural stem populations and must be down - regulated for neural progenitor differentiation to proceed ( bylund et al .
, 2003 ; kishi et al . , 2000;mizuseki et al . , 1998a ; penzel et al . ,
each maintains neural stem cells in a proliferative state upstream of neuronal differentiation genes ( bani - yaghoub et al .
, 2006 ; ellis et al . , 2004 ; graham et al . , 2003 ; li et al .
likewise , sox11 , a member of the soxc subfamily , is up - regulated as neural stem cells transition to neural progenitor cells , and later maintains neuronal progenitors ( bergsland et al . , 2006 ; uwanogho et al . ,
thus , together these sox genes may function downstream of foxd4 , gmnn and zic2 to promote the initial step from neural plate stem cell to neural progenitor cell .
several studies show that other zic genes and the irx genes function in neural progenitors downstream of the soxb1 genes to promote the onset of bhlh neural differentiation gene expression .
zic1 is required for the expression of soxd ( a member of the soxg group ) , which causes ectopic neural masses that express bhlh neural differentiation genes ( mizuseki et al . , 1998b ) , and zic1 and zic3 promote the expansion of neural progenitors in the spinal cord and forebrain ( aruga et al . , 2002 ; nakata et al . , 1997 ; 1998 ) .
in drosophila , iroquois genes are required for the activation of the proneural bhlh genes ( gomez - skarmeta et al . , 1996 ) , and in xenopus
the homologous irx / xiro genes are expressed just prior to the earliest expressed bhlh neural differentiation genes(bellefroid et al . , 1998 ) . while irx genes promote the onset of neural differentiation ( bellefroid et al . , 1998 ;
1998 ) , they also suppress terminal differentiation into neurons ( de la calle - mustienes et al . , 2002 ) .
these studies demonstrate that the ntfs have important roles in maintaining a ne precursor state , regulating the size of the neural plate stem cell population and controlling the onset of differentiation of the neural progenitor cells .
however , their functions in cultured stem cells has not been extensively explored . to date , most emphasis has been put on forcing stem cells to a neural fate and then differentiating them into specific neuronal and glial types ( chan et al . , 2014 ; ross and akimov , 2014 ; wichterle et al . , 2002 ) .
however , a common problem with these protocols is the difficulty in maintaining neural precursors in an immature , undifferentiated state . for many therapeutic applications this would be advantageous because it would expand neural precursor cells without the spontaneous and premature differentiation that commonly occurs in these cultures . in the embryo ,
next , as the neural plates forms , these cells transition into neural plate stem cells which subsequently differentiate into regionally - specified neural progenitor cells . whereas in the embryo , proliferative , undifferentiated neural precursors can be maintained , it is rare to see this kind of cell in esc cultures .
however , similar primitive neural precursors do form in mouse esc cultures when neural inductive signaling is maintained either by adding anti - bmp factors or growing cells at a low enough density to dilute endogenously produced bmps ( tropepe et al . , 2001 ) .
the molecular mechanisms by which this primitive state is acquired and maintained , and by which these cells transition to the better characterized neural stem cell state will be important to elucidate in light of the potential to eliminate tumor - producing pluripotent cells .
the studies summarized above took a gene - by - gene approach to understand the functions of each ntf during the progressive transition from neural inductive signaling to neural progenitor differentiation . based on their sequential and overlapping activities it seems likely that the ntfs coordinately function in a gene regulatory network ( fig .
2 ) . an initial set of ntfs maintains cells in a stable , immature ne precursor state that proliferates to form an appropriately sized neural plate .
these are then down - regulated and a different set of ntfs is activated to promote the formation of the neural stem cell constituents of the neural plate .
finally , neural stem cell ntfs are down - regulated and a third set of ntfs is activated that initiate neural differentiation .
our understanding of the functional and transcriptional relationships between these proteins is woefully incomplete , yet this information is fundamental for understanding how the balance between neural precursor , neural stem and neural progenitors is molecularly regulated .
developmental events often are controlled by regulatory networks of transcription factors that control temporal and region - specific gene expression ( levine and davidson , 2005 ) .
therefore , we sought to place these ntfs in a network to determine their distinct roles in the progression from ne precursors to differentiating neural progenitors by analyzing the epistatic position of foxd4 amongst these other ntfs . using protein knock - down approaches we found that foxd4 is required for the expression of each of the other ntfs(yan et al . , 2009 ) , thus placing it upstream in the network ( fig .
2 ) . using gain - of - function approaches , we found that elevated foxd4 levels in ne precursors up - regulated gmnn and zic2 , delayed the expression of sox2 , sox3 and sox11 , which resulted in an expanded neural plate ; and down - regulated the zic and irx genes that promote neural progenitor differentiation .
we also found that foxd4 expression is down - regulated at early neural plate stages , suggesting that its repression is required for ne precursors to progress to neural plate stem cells and to neural progenitors .
additional experiments demonstrated that gmnn , zic2 and sox11 together can mediate the foxd4 effects on the remaining ntfs(yan et al . , 2009 ) .
from these results we can construct a gene regulatory network in which foxd4 is a critical upstream component .
it directly activates a transcriptional triad consisting of gmnn , zic2 and sox11 , which in turn regulates the more down - stream components that promote the transition to neural stem and neural progenitor cells ( fig .
, structure - function analyses of the foxd4 protein show that it has separate domains in the n - and c - terminal regions that account for its ability to both activate or repress targets ( klein et al .
these findings illustrate how a single transcription factor can regulate the transition of immature , ne precursors to neurally - committed stem cells , and then to neural progenitors that are beginning to differentiate .
the experiments described above demonstrate that foxd4 is a critical element in the gene regulatory network that regulates the balance between ne precursors , neural plate stem cells and regionally specified neural progenitor cells . elucidating how these different ntf genes interact to regulate this balance between expanding precursor populations and initiating differentiation should ultimately prove useful for preventing the formation of mesodermal and endodermal cells in neuronal culture protocols , expanding the number of neural precursors available and preventing their premature differentiation .
one would predict from the experiments performed in embryos that during the differentiation of escs into nscs foxd4 , gmnn , zic2 and sox11 would act early to promote immature , proliferative ne precursors and impede neural differentiation ( fig .
our preliminary work analyzing the role of foxd4 in embryoid bodies derived from mouse escs and differentiated along a neural lineage indicates that this protein is key to the transition between pluripotency and neurally committed cells , just as in the embryo ( moody et al .
, gmnn promotes an uncommitted state by promoting polycomb mediated repressive histone marks at differentiation - promoting genes ( lim et al . , 2011 ) .
it also prevents premature neurogenesis by antagonizing the interaction between brg and bhlh neural differentiation factors ( seo et al . , 2005 ) .
just like in the embryo , in mouse escs gmnn is required for the acquisition of a neural fate , and can promote the formation of neural precursor cells by maintaining the chromatin in a hyper - acetylated state and in an open conformation that allows other ntfs access to their binding sites on the dna ( yellajoshyula et al . ,
subsequent work showed that gmnn promotes both activating and repressive histone modifications at neural differentiation genes , suggesting that gmnn makes the chromatin accessible to both factors that promote ne precursors as well as to factors that will subsequently activate neural differentiation genes ( yellajoshyula et al . , 2012 ) .
these results explain why gmnn - deficient neural progenitors in the mouse cortex are defective in cell proliferation and differentiation ( spella et al . , 2011 ) , whereas gmnn - deficient nscs in neurospheres can still divide and differentiate into neurons and glia ( schultz et al . , 2011 ) . whereas the role of zic2 has not been examined in esc culture ,
soxb1 family proteins ( sox1 , sox2 , sox3 ) are expressed throughout mouse and chick embryo ne precursors and neural plate stem cells , and when expressed at high levels they prevent cells from differentiating into neurons ( uwanogho et al . , 1995 ; wegner , 1999 ) .
in addition , sox2 has an earlier function in escs : maintenance of pluripotency(avilion et al . , 2003 ) .
sox11 is expressed through - out ne precursors and neural plate stem cells , overlapping completely with sox2 and sox3 expression in xenopus ( hyodo - miura et al . , 2002 ) , whereas in chick and mouse embryos , it is expressed later in neural progenitors and differentiating neurons , and does not overlap with sox2 or sox3 expression in the neural tube ( uwanogho et al .
, 1995 ; wegner , 1999 ) ; in mouse it also is expressed in several other tissues to support progenitor cell survival ( bhattaram et al . , 2010 ) .
a recent study showed that sox2 , sox3 and sox11 bind to the promoters / enhancers of the same neural genes expressed in mouse nscs , neural progenitors and differentiating neurons in a defined temporal sequence ( bergsland et al . , 2011 ) .
in escs , sox2 binds to nsc genes to mark sites for later sox3 binding .
subsequently in nscs , sox3 binds to neuronal differentiation genes to mark sites for later sox11 binding . in each case , sox protein binding results in biva - lent chromatin marks , indicating that part of their effect is making the appropriate neural genes accessible to other transcriptional factors that are expressed later .
there is a large literature that describes the ntfs that are involved in neural progenitor cell ( npc ) formation that has been extensively reviewed elsewhere ( florio and huttner , 2014 ; imayoshi and kageyama , 2014 ) . as mentioned above ,
the bhlh factors , particularly neurog and neurod are critical for npcs to exit the cell cycle and activate neuron or glial specific genes .
in addition , pattern formation genes are important in providing npcs with their spatial address within the neural tube . depending upon whether the npc is located in the anterior or posterior , dorsal or ventral parts of the neural tube , the expression of patterning genes will impact whether it differentiates into the forebrain versus spinal cord , and sensory versus motor neuron .
a large literature describes the signaling and transcriptional factors that are responsible for differentiating neural progenitor cells into the wide range of neurons and glia that are responsible for the complex function of the vertebrate central nervous system .
these factors are important for understanding how the brain functions , and for designing stem cell protocols to replace specific cells affected by neurodegenerative disease and injury .
in contrast , much less in know about the molecular regulation that guides a newly induced neural ectodermal cell to commit to its new fate , expand the neural plate stem cell population and transition to a neural progenitor cells . as work in the embryo reveals more details about the regulation of these earliest steps in neural development , we need to test these details in cultured stem cell paradigms to facilitate the manipulation of nscs for therapeutic uses .
it is now important to test these possibilities experimentally , and elucidate the essential gene regulatory network in mammalian esc - and ipsc - derived neural cell cultures . using the information we have obtained from the embryo
 the early steps of neural development in the vertebrate embryo are regulated by sets of transcription factors that control the induction of proliferative , pluripotent neural precursors , the expansion of neural plate stem cells , and their transition to differentiating neural progenitors . 
 these early events are critical for producing a pool of multipotent cells capable of giving rise to the multitude of neurons and glia that form the central nervous system . in this review 
 we summarize findings from gain- and loss - of - function studies in embryos that detail the gene regulatory network responsible for these early events . 
 we discuss whether this information is likely to be similar in mammalian embryonic and induced pluripotent stem cells that are cultured according to protocols designed to produce neurons . 
 the similarities and differences between the embryo and stem cells may provide important guidance to stem cell protocols designed to create immature neural cells for therapeutic uses . 
INTRODUCTION
NEURAL INDUCTIVE SIGNALING
Transitioning to neural plate stem and neural progenitor cells
Expansion of neural plate stem cells
A neural plate gene regulatory network
Neural progenitor differentiation
CONCLUSION