there are at least 40 types of legionella bacteria , half of which are capable of producing disease in humans . the legionella pneumophila bacterium
we describe the case of a 60-year - old woman with a history of diabetes mellitus and arterial hypertension who was admitted to our hospital with fever and symptoms of respiratory infection , diarrhea , and acute renal failure .
we used real - time polymerase chain reaction ( pcr ) to detect l. pneumophila dna in peripheral blood and serum samples and urine antigen from a patient with pneumonia .
since legionella is a cause of 2% to 15% of all community - acquired pneumonias that require hospitalization , legionellosis should be taken into account in an atypical pulmonary infection and not be forgotten . moreover , real - time pcr should be considered a useful diagnostic method .
legionella species are gram - negative bacteria that are ubiquitous in both natural aquatic and moist soil and muddy environments and in artificial aquatic habitats.1,2 human infection with legionella spp .
has two distinct forms : legionnaires disease , a more severe form of infection which includes pneumonia , and pontiac fever , a milder febrile flu - like illness without pneumonia.3 legionella stands as the cause of community- acquired pneumonia ( cap ) in 2%15% of all caps that require hospitalization .
the clinical and radiological features of legionella pneumonia are nonspecific , and the diagnosis depends on laboratory tests.4 this paper reports a case of pneumonia caused by legionella pneumophila that was admitted to the general hospital of komotini in greece .
a 60-year - old female with a history of diabetes mellitus , chronic obstructive pulmonary disease , and arterial hypertension with a 4-day history of watery diarrhea and temperature was admitted to our hospital .
the findings from physical examinations on admission were as follows : temperature 38.6c , blood pressure 130/95 mm hg , heart rate 117 beats / min , and respiratory rate 28 breaths / min .
laboratory findings on admission were as follows : white blood cell ( wbc ) count was 14,280/l , creatinine was 3.4 mg / dl , the erythrocyte sedimentation rate ( esr ) was 110/h , procacitonin ( pct ) 2 ng / ml , c - reactive protein ( crp ) 14 mg / dl ( table 1 ) .
we considered the elevated creatinine levels as acute , because at her previous laboratory tests her creatinine values never surpassed 1.3 mg / dl .
arterial blood gas showed hypoxemia , as partial pressure of oxygen on air was 56 mm hg.5 the chest x - ray showed infiltration of the left lung ( figure 1 ) . with a clinical diagnosis of pneumonia ,
urine samples for l. pneumophila and streptococcus pneumoniae antigen , gram stain sputum , and blood specimens upon admission were collected from the patient for culture testing.6 the results were reported negative . the pneumonia severity index ( psi )
was evaluated as class 3 with a mortality rate of 0.9%.7 the patient was treated empirically with 1 g amoxycillin / clavulanic acid three times daily and 500 mg clarithromycin two times daily for 2 days .
empiric antibiotic treatment was added upon admission based on elevated values of crp , wbc , esr , and chest x - ray findings , since early antibiotic treatment prevents progression of the disease and these markers are known to be elevated in infectious diseases.8,9 the clinical status of the patient was deteriorating , and there was a marked progression of the infiltrates on the chest x - ray ; the left infiltrate progressed to bilateral shadows .
the laboratory values were as follows on the 2nd hospital day : wbc count 17,780/l , crp 18 mg / dl , pct 2 ng / ml , and esr 120/h .
the patient s hypoxemia increased : partial pressure of oxygen in arterial blood ( pao2 ) 47 mmhg , partial pressure of carbon dioxide in arterial blood ( paco2 ) 25.4 mm hg , and ph 7.5 ( table 1 ) .
one day after her admission to hospital ( day 2 ) ( figure 2 ) , two blood samples , one serum sample and a second urine sample were taken and tested for l. pneumophila with a real - time polymerase chain reaction ( pcr ) kit ( aqua screen l. pneumophila - detection kit for real - time pcr ; minerva biolabs , minerva , oh ) and with culture method .
a portion of 200 l of blood and an equal volume of serum were plated on buffered charcoal yeast extract ( bcye ) ( oxoid , reading , uk ) with l - cysteine , on bcye without l - cysteine , and on gvpc ( gas vesicle protein c ) .
the antibiotics were immediately changed to levofloxacin after the positive real - time pcr and legionella urinary antigen detection test .
the patient responded after therapy with levofloxacin , on day four ( figure 3 ) .
her general condition , as well as radiographic and laboratory findings , gradually improved and the patient was discharged on the 21st day of hospitalization ( figure 4 ) . to identify the source of the infection , the patient s relatives were interviewed .
exposure histories revealed that the patient had travelled abroad and visited an operating spa pool 2 weeks before the day of onset .
after she returned , she remained in her village until her admission to hospital . as there were no cooling towers or any aerosol - generating systems in an area of 2 km from the house , and there was no access to the spa pool abroad , environmental samples were taken only from the home water supplies , and standard methods were processed .
the incidence of legionnaires disease has increased in the last decade since the introduction of urinary antigen immunoassays.10,11 this test accounts for most of the diagnostics due to its high sensitivity and ease of use.12 l. pneumophila has become one of the leading causes of cap in adults , accounting for 6%14% of cases requiring hospitalization in recent studies.13,14 legionnaires disease occurs sporadically and in outbreaks , with the sporadic form representing 65%82% of the cases.10,11,15 nevertheless , the number of confirmed community outbreaks including more than 100 cases has increased in recent years due to the use of legionella antigenuria.11,15 routine testing for legionella urinary antigen has increased the number of diagnostics of legionnaires disease and has allowed earlier diagnosis and treatment , greatly improving the prognosis.16 this has been particularly true for milder cases , mainly in the outbreak setting.17 however , most of the knowledge on risk factors , clinical presentation , and outcome of community - acquired legionnaires disease is based on studies performed before routine urinary antigen testing was adopted.18,19 moreover , recent community outbreaks have contributed to the better understanding of legionnaires disease in this setting.2022 legionella pneumophila has been recognized as an important cause of both cap and nosocomial pneumonia.1,10,11,23 environmental systems , such as air conditioning cooling towers , evaporative condensers , whirlpools , and hot spring baths have hosted and transmitted the organism .
cases of legionella pneumonia presumably transmitted from contaminated hot spring spa water have been reported from greece.23 the early recognition of infection due to legionella plays a major role in its treatment and preventing mortality in patients with any underlying disease.24,25 in this case , the pcr method was lifesaving for the patient because it confirmed the infection , leading to the administration of the right treatment , whereas the first urine antigen test was misleading .
this way of diagnosing legionella has been well established in previous published studies , and it should be applied in such cases where a differential diagnostic problem exists.26,27 legionnaires disease is an acute bacterial infection generally caused by l. pneumophila , primarily involving the lower respiratory tract .
outbreaks have been described related to a common source of contamination.1 erythromycin has been the treatment of choice ever since a retrospective study of the original outbreak in philadelphia indicated a lower mortality rate with this antibiotic . because legionella is an intracellular pathogen , antibiotics that penetrate intracellularly are likely to be active against this pathogen . both fluoroquinolones and macrolides penetrate cells well , and
fluoroquimolones achieve high intracellular levels and have a lower minimum inhibitory concentration against legionella than erythromycin.28 they are more active than erythromycin in inhibiting l. pneumophila in different intracellular models . three observational studies with a total of 458 patients have indicated that fluoroquinolones ( mainly levofloxacin ) are associated with a superior clinical response when compared with that of macrolides ( erythromycin and clarithromycin ) , as evidenced by a shorter time to apyrexia , shorter hospital stay , and fewer drug - related complications.2931 however , no randomized trials have been performed yet .
although it has been traditional to add rifampin to erythromycin to treat severe legionellosis , an observational cohort study revealed that the addition of rifampin to clarithromycin was associated with more side effects and a longer hospitalization stay than erythromycin alone.30,32 in all cases of severe , life - threatening pneumonia , prompt administration of appropriate antibiotics is associated with improved outcomes .
this case should urge every clinical doctor to study carefully the medical history of a patient and consider alternative diagnosis when the patient is not responding to the initial treatment .
flouoroquinolones have established an equal if not superior therapeutic profile for legionellosis based upon published studies.2830 it should be mentioned at this point that a very important limitation of the study was that we were unable to take water samples from the spa in order to have a solid confirmation of the source of legionellosis .
pcr is a useful tool in the hands of the clinical doctor and should be used where possible in suspicious cases , such as the above .
also , early antibiotic treatment prevents the progression of the infection and should be administered upon admission .
finally , flouoroquinolones , and in this case levofloxacin , should be considered an effective and efficacious treatment for legionellosis .
written informed consent was obtained from the patient s next - of - kin for publication of this case report and any accompanying images .
 introductionthere are at least 40 types of legionella bacteria , half of which are capable of producing disease in humans . the legionella pneumophila bacterium 
 , the root cause of legionnaires disease , causes 90% of legionellosis cases.case presentationwe describe the case of a 60-year - old woman with a history of diabetes mellitus and arterial hypertension who was admitted to our hospital with fever and symptoms of respiratory infection , diarrhea , and acute renal failure . 
 we used real - time polymerase chain reaction ( pcr ) to detect l. pneumophila dna in peripheral blood and serum samples and urine antigen from a patient with pneumonia . 
 legionella dna was detected in all two sample species when first collected.conclusionsince legionella is a cause of 2% to 15% of all community - acquired pneumonias that require hospitalization , legionellosis should be taken into account in an atypical pulmonary infection and not be forgotten . 
 moreover , real - time pcr should be considered a useful diagnostic method . 
Introduction
Case presentation
Conclusion
Introduction
Case report
Discussion
Conclusion
Consent