surface sampling was conducted by using swabs , wipes , and hepa vacuum socks . to compare the sampling techniques , we selected locations where the three types of samples could be collected adjacent to each other on nonporous surfaces , with an emphasis on locations believed to be still contaminated with b. anthracis spores .
17 ) , return air ducts , tops of the window boxes along the postal inspector walkways , and the tops of mail sorting bins in a secure area approximately 23 m from dbcs machine no . 17 .
the order in which the samples were collected varied in a randomized fashion from location to location ; each site was assigned a location number and sampled according to a predetermined , randomized plan .
we used the randomized sampling plan to reduce sampling biases that might be caused by nonuniform distribution of spores across surfaces and affected by the order in which samples were collected .
seven swab , six wipe , and five hepa vacuum sock samples were collected as control samples ; that is , these samples were handled in the same way as others but not used to sample any surfaces .
the purpose of these control samples was to evaluate the potential contamination of sample media , unrelated to actual sample collection .
nine additional blank hepa vacuum sock samples were collected to estimate cross - contamination by inserting them into the vacuum nozzle after a hepa vacuum sock sample had been collected and the nozzle cleaned ; these socks were then withdrawn and placed in a sterile conical tube for laboratory analysis .
investigators were given written instructions for collecting samples at each selected location ( figure 1 ) .
the surface areas sampled by each technique were intended to be comparable , but not necessarily equal . in particularly dirty areas , swabs and wipes could not cover as large a surface area as the hepa vacuum sock samples without becoming overloaded ; investigators were instructed to avoid overloading the samples by adjusting the size of the surface sampled .
sample instructions for collection of swab , wipe , and hepa vacuum sock samples , brentwood mail processing and distribution center , 2001 . for specific location
divide the selected space into three sections where each of the three types of surface samples ( swab , wipe , hepa vacuum sock ) may be collected .
follow the random key above to designate which section will be sampled by each method and in which order the samples will be collected ( follow top to bottom ) .
the surface areas need not be equal , but should be sufficient to provide adequate sample collection for each method .
sample order for location was : 1 ) collect the hepa vacuum sock sample first and record surface area .
after sampling , clean vacuum nozzle with alcohol and insert clean vacuum sock ; remove this sock without sampling to serve as  contamination blank .
4 ) collect an additional wipe sample across the entire area which had been sampled by hepa vacuum sock .
5).collect an additional hepa vacuum sock sample across the entire which had been sampled by wipe .
the following procedures , used to collect the three types of surface samples , were recommended for collecting surface environmental samples for culturing b. anthracis 
 ( 9 ) .
the surface samples were all collected after investigators had donned nonpowdered gloves over two pairs of nitrile protective gloves , as part of the personal protective gear .
the area of the surface sampled was measured with a tape measure and recorded in square centimeters .
swab samples were collected by removing a sterile , rayon ( noncotton ) swab ( environmental swab kit , cdc , atlanta , ga ) from a sterile tube , moistening it by inserting it into a second tube which contained a sponge soaked with sterile 1.5 ml of phosphate - buffered saline ( pbs ) at ph 7.2 , and then swabbing the selected surface by moving the swab back and forth across the surface with several horizontal strokes , then several vertical strokes .
the swab was rotated during sampling to ensure that the entire surface of the swab was used .
after sampling , the swab was returned to its original , prelabeled sampling tube for submission to the laboratory . at every selected location ,
approximately half the sites were also sampled again with unmoistened dry swabs to compare the sampling efficiency of dry swabs to wet swabs and other techniques .
wipe samples were collected on selected surfaces with a 7.62 x 7.62 cm sterile rayon gauze pad ( dukal corp . , syosset , ny ) premoistened with approximately 5 ml sterile water ( baxter healthcare corp . , deerfield , il ) .
the surface was thoroughly wiped back and forth by using several vertical strokes , folding the exposed side of the pad , and making several horizontal strokes over the same area with the other side of the wipe .
the pad was then placed in a prelabeled , 50-ml sterile conical tube and sealed with a cap .
hepa vacuum sock samples were collected by inserting a cone - shaped filtering trap ( dust collection filter sock ; midwest filtration co. , fairfield , oh ) into the nozzle of a hepa vacuum cleaner ( atrix international inc . ,
the vacuum had an electric motor ( 120 v , 6.6 a , 1 hp ) to provide suction of 28 cubic feet ( 792.4 l ) per min through the vacuum nozzle ( figure 2 ) .
the plastic sleeve of the dust collection trap was folded over the outside of the nozzle and held in place by hand while the vacuum nozzle was moved slowly back and forth across the sampled surface .
the dust collection trap was removed from the vacuum nozzle , placed in a prelabeled , 50-ml sterile conical tube , and sealed with a cap . before inserting a clean sock into the vacuum nozzle and collecting a subsequent sample ,
the investigator put on a new pair of gloves and wiped the inside of the vacuum nozzle thoroughly with an alcohol wipe , to physically remove contamination from the nozzle surface ( not to sterilize the surface because alcohol does not effectively kill b. anthracis spores ) . to determine whether cross - contamination of subsequent vacuum samples might occur through contamination of the vacuum nozzle during sampling ,
occasionally a filter sock was inserted into the vacuum nozzle after a sample had been collected and the nozzle cleaned , but the sock was then simply withdrawn and placed in a sterile conical tube for laboratory analysis .
swab and wipe samples were extracted in a laboratory operated by the usps contractor at the brentwood facility .
the samples were extracted by adding 2030 ml 0.3% tween 20 in pbs to a 50-ml blue falcon screw - top tube ( becton dickinson labware , franklin lakes , nj ) and vortexing the tube for 3 min .
the contents of the tube were allowed to settle for 5 min , and swabs and wipes were removed .
the tube was centrifuged at 3,0004,500 rpm , 1530 min at 10c , the supernatant removed by decanting , and the pellet was resuspended in 2 ml 0.3% tween 20 in pbs solution .
approximately half the resuspended extract was shipped to cdc bioterrorism surge capacity and anthrax laboratories for culture and confirmatory analysis .
the remaining half of the resuspended extract was retained at the laboratory at the brentwood facility for polymerase chain reaction ( pcr ) analysis ( unpub .
0.1 ml of the suspension ( approximately 10% of the extract solution ) was plated to trypticase soy agar with 5% sheep blood and streaked for quantification .
the plates were incubated at 35c37c in ambient air and examined after 24 h and 48 h. suspect colonies were screened by level a procedures for identification of b. anthracis ( 11 ) .
identification of all strains was confirmed by standard microbiologic procedures and the laboratory response network ( lrn ) testing algorithm ( 12,13 ) .
results of these samples were reported as number of cfus per plate . to estimate cfus per sampled surface area ,
the number of cfus per plate was multiplied by 20 ( 2 ml extract solution divided by 0.1 ml plated solution ) and divided by the recorded surface area in square centimeters .
when the number of colonies on the culture plates exceeded approximately 300 , they were reported as too numerous to count .
we used the average weight of five unused sock samples to estimate the presampling weight of the vacuum socks ; the average weight of the unused socks was 0.70 g ( standard deviation 0.02 g ) .
the average weight was subtracted from the postsampling weight of each sock sample to determine the weight of its contents .
approximately 2030 ml 0.3% tween 20 in pbs was added to a 50-ml cup containing the sock and its contents and placed on a shaker for 30 min .
the contents of the cup were allowed to settle for 5 min ; the supernatant then was poured into a 50-ml blue falcon screw - top tube ( becton dickinson labware ) .
the tube was centrifuged at 3,0004,500 rpm , for 1530 min at 10c ; approximately 90% of the starting volume was then removed .
the pellet in the bottom of the tube was resuspended in approximately 2 ml 0.3% tween 20 in pbs , and 0.1 ml ( two drops from pasteur pipette ) and 0.01 ml ( added by using a calibrated loop ) of the suspension were plated to two trypticase soy agar plates with 5% sheep blood and streaked for quantification .
the plates were incubated and screened , and suspect colonies were identified by using the same laboratory methods used for the swab and wipe samples .
results of these samples were reported as cfu / g of material collected ; the estimated weights of the sock contents were also reported . to estimate cfu per sampled surface area , the reported cfu /
g were multiplied by the reported weight of the sock contents and divided by the recorded surface area in square centimeters . to evaluate the effectiveness of the wipe and hepa vacuum samples for removing spores from surfaces , at some locations we collected wipe samples over the same surface area previously vacuumed , as well as hepa vacuum samples over the same surface area previously wiped .
we compared the relative difference in cfu / cm reported for the two methods to evaluate the removal efficiency of the wipe and hepa vacuum sock samples .
operations to decontaminate the brentwood facility had been done since late october 2001 by using hepa vacuums and sodium hypochlorite solutions .
swab , wipe , and hepa vacuum sock samples of dbcs machine surfaces that had been cleaned were collected to evaluate the effectiveness of clean - up operations .
sample results ( positive vs. negative ) were analyzed by using simple descriptive statistics , including counts and percents .
agreement between paired sampling methods was assessed by using cohen s kappa , a statistical method that measures agreement beyond what would be expected based on chance alone ( 15 ) .
kappa scores < 0.4 were considered poor agreement , while scores > 0.75 indicated excellent agreement ; kappa scores between these values indicated fair to good agreement .
sample levels ( cfu / cm ) were analyzed by simple descriptive statistics , including sample median and range .
spearman s rank correlation coefficient significance tests based on ranks that do not assume normality were used as a measure of the association between two paired sampling methods ( 16 ) . agreement between paired sampling methods with respect to ordered categories ( 0 , 0.11.6 , 1.715.5 , and > 15.5 cfu / cm ) was assessed by using kendall s tau - b statistic , which measures ordinal association ( 17 ) .
descriptive statistics for the culture analysis of the dry and wet swab , wipe , and hepa vacuum sock samples are shown in table 1 .
b. anthracis was cultured from 4 ( 14% ) of 28 dry swab samples , while 36 ( 54% ) of 67 wet swabs were culture positive .
fifty - eight ( 87% ) of 67 of the wipe samples and 51 ( 80% ) of 64 of hepa vacuum sock samples were culture positive .
although cfus / cm were reported for each positive sample , these results should only be considered semiquantitative ; absolute concentrations can not be directly compared across the sampling methods . however , the calculated concentrations of b. anthracis spores in the culture - positive hepa vacuum sock samples tended to be greater than in the other types of samples . level of b. anthracis ( cfu / cm ) : negative = 0 , low = 0.11.6 , medium=1.715.5 , and high=>15.5 .  positive samples only .  232.5 cfu / cm is the maximum value considered too numerous to count for a concentration ; 300 cfu is the maximum value considered too numerous to count for a culture .
of the nine blank hepa vacuum samples collected from the vacuum nozzle to estimate cross - contamination , eight were culture negative ; one b. anthracis cfu was detected in one sock .
the results of the dry swab samples are compared with results obtained by using the other types of samples ( table 2 ) .
these results indicate that when corresponding wipe and hepa vacuum sock samples were culture positive for b. anthracis spores , the dry swab samples detected b. anthracis 4 of 23 times . when the corresponding wet swabs were positive for b. anthracis spores , the dry swabs detected b. anthracis 4 of 13 times . at no time were the dry swabs positive while the other types of corresponding samples did not detect spores .
two samples from the same location are concordant if both positive or both negative for bacillus anthracis spores . 
two samples from the same location are discordant if one is positive and the other negative for b. anthracis spores . 
rs denotes spearman s correlation coefficient between level of b. anthracis ( cfu / cm ) obtained by using the dry swab method and the level of b. anthracis obtained by using the comparison sampling method . 
. a total of 58 sets of wet swab , wipe , and hepa vacuum sock samples collected side - by - side were available for comparison , and 67 sets of wet swab and wipe samples collected side - by - side were also available for comparison ( table 3 ) .
results of wet swab and wipe sample analysis were concordant in 64% of the sample comparisons ; 23 wipe samples were reported as culture positive when the wet swab samples failed to detect spores , and 1 culture - positive wet swab sample was reported when the corresponding wipe sample was culture negative .
results of the wet swab and hepa vacuum sock samples were also concordant on 64% of the sample comparisons with similar results as the wet swab and wipe comparison .
twenty - one ( 36% ) hepa vacuum samples were reported as culture positive when the wet swab samples were negative , but no culture - positive wet swab samples were ever reported when the corresponding hepa vacuum sock samples were negative .
results of hepa vacuum sock and wipe samples were concordant 84% of the time ; when they were discordant , the corresponding hepa vacuum sock and wipe samples did not detect b. anthracis spores about the same number of times ( five negative for hepa vacuum sock and four negative wipe samples ) .
only the comparison of hepa vacuum sock versus wipe sample had a cohen s kappa score > 0.4 , indicating fair to good agreement ( table 3 ) . two samples from the same location
are concordant if both positive or both negative for presence of bacillus anthracis spores . 
two samples from the same location are discordant if one is positive and the other negative for presence of b. anthracis spores .
the hepa vacuum sock samples typically collected higher concentrations of b. anthracis spores than both the wet swab and wipe samples , and the wipe samples collected higher concentrations of spores than the wet swab samples ( table 4 ) .
these comparisons indicate good agreement between the hepa vacuum sock samples and the wipe samples ( kendall s tau - b 0.66 ; spearman s rank correlation coefficient 0.81 ) .
although wet swabs were correlated with both the hepa vacuum samples and the wipe samples , the agreement was not as strong .
level of b. anthracis ( cfu / cm ) : negative = 0 , low = 011.6 , medium=1.715.5 , and high=>15.5 .  two samples from the same location agree if they are concordant and are both in the same grouping . 
the randomly selected surface areas where 13 hepa vacuum sock samples had been collected were immediately sampled again with wipe samples .
all the hepa samples were positive for b. anthracis spores ranging in concentrations from 0.5 to 310 cfu / cm .
the spore concentrations collected by the subsequent wipe samples ( 0 to 16 cfu / cm ) were usually lower than the original vacuum samples ; only two of the subsequent wipe samples were negative for b. anthracis spores .
the surface areas where 12 wipe samples were collected , corresponding to 12 of 13 hepa vacuum sock samples , were immediately sampled again with hepa vacuum sock samples .
all the wipe samples were positive for b. anthracis spores , ranging in concentrations from 1.4 to 233 cfu / cm .
only one of the subsequent hepa vacuum samples was negative for spores and the concentrations in nine of the hepa vacuum sock samples were virtually the same as on the original wipe samples .
the results of the side - by - side comparison of swab , wipe , and hepa vacuum sock samples on nonporous surfaces indicated good agreement between the hepa vacuum sock and wipe samples .
however , the hepa vacuum sock and wipe samples agreed poorly with the swab samples .
the wet swabs did not detect spores > 33% of the time when spores were detected by the wipe and vacuum sock samples .
the dry swabs performed especially poorly , failing to detect spores > 66% of the time when spores were detected by wipe and vacuum sock samples . based on these results , dry swabs should not be used to sample for b. anthracis environmental contamination .
applying wet swabs in certain circumstances may be useful , for example , to sample crevices , inside machinery , and places difficult to reach by wipe and hepa vacuum samples ; however , dry swabs should not be used to sample surfaces where wipe and hepa vacuum samples are likely to yield superior results .
sampling with wipes and hepa vacuum socks is likely to yield very similar results on nonporous surfaces ; wipes are preferable for sampling surfaces with relatively light dust , while hepa vacuum socks should be selected to sample surfaces with heavy dust .
wipes may become quickly overloaded on dusty surfaces and thus unable to cover a large surface area .
the sampling sensitivity of hepa vacuum socks may be greater because they can collect large dust loads over much larger surface areas than wipes .
the relative difference between the wipe samples and the subsequent hepa vacuum sock samples was not influenced by the initial concentration of spores collected by the wipe samples .
after especially dirty areas were sampled with both wipes and hepa vacuum sock samples , residual dirt was often still visible .
the samples were collected side by side so that the exact same surface area was not sampled by all methods .
because of nonuniform distribution , spore concentrations may have varied across the surfaces sampled by each method .
however , we also set the order of sampling as random , making it unlikely that any particular method consistently encountered fewer spores than the other methods .
strong differences in these particular results more likely resulted from the sampling technique and not to nonuniform distribution of spores on these highly contaminated surfaces , where the different types of samples were collected very close to each other . in areas likely to have been contaminated over a broad surface at high concentrations ( such as dbcs machine no .
17 ) , an adequate number of spores for detection was likely available for all three sampling techniques , but in other , less - contaminated areas , fewer spores were available for detection .
if investigators are careful to avoid contamination of the samples , the number of false - positive samples is reduced .
however , sampling all surfaces within a building is not practical , and some surfaces containing b. anthracis spores might be missed .
the measurements collected in this study were not adequate to evaluate the sampling efficiencies of wipe and hepa vacuum sock samples , particularly since the initial concentrations of spores on the sampled surfaces were unknown . however , sequential hepa vacuum sock samples indicated better collection efficiency on nonporous surfaces than wipe samples .
this efficiency is evident because wipe samples collected following vacuum samples were much lower than the initial vacuum samples , while the vacuum samples collected after wipe samples often collected a similar concentration of spores as the initial wipe samples .
care was taken after sampling to stay within the previously sampled area , but spores from outside the previously sampled area may have been inadvertently collected by the hepa vacuum samples ( e.g. , spores from surrounding unsampled areas may have been drawn into the hepa vacuum sock ) . to avoid contamination of the vacuum when
collecting samples , using disposable inserts may be more appropriate , such as cardboard sleeves , which can be placed inside the vacuum nozzle ; the sampling sock can then be inserted into the sleeve and discarded after sampling .
disposable inserts may prevent cross - contamination of the vacuum nozzle or subsequent sock samples .
care must be taken to prevent contamination of the inserts before they are used for sampling . while vacuum nozzles may not always be completely cleaned after sampling , our investigation indicated that cross - contamination could not be the reason for the high concentrations of spores detected on the numerous hepa vacuum sock samples .
the results of this investigation may be used to guide future sampling efforts and serve as a baseline for follow - up measurements after the building has been cleaned further .
the sampling and analytical techniques used in our study may provide useful reference for evaluations of other situations in the future .
this study provides additional evidence for the need to quantify sampling efficiency to develop the type of limit - of - detection data normally created for other types of sampling and analytical methods . the collection efficiency ( removing spores from the surface ) and recovery efficiency ( removing spores from the sampling media ) need to be further evaluated for these methods .
our study focused on sampling nonporous surfaces ; under these circumstances , hepa vacuum sock samples and wipe samples performed similarly .
however , this level of agreement may be difficult to achieve in sampling porous materials such as carpet and furniture , and the collection efficiency of sampling methods on other surfaces needs to be evaluated . understanding the sampling efficiency of these methods on various types of surfaces is a critical requirement for future efforts to develop numerical criteria for surface contamination and potential exposures to humans .
lack of understanding about the efficiency of various sampling methods limits our ability to determine whether an environment has been adequately cleaned .
 during an investigation conducted december 1720 , 2001 , we collected environmental samples from a u.s . postal facility in washington , d.c . , 
 known to be extensively contaminated with bacillus anthracis spores . because methods for collecting and analyzing b. anthracis spores have not yet been validated , our objective was to compare the relative effectiveness of sampling methods used for collecting spores from contaminated surfaces . 
 comparison of wipe , wet and dry swab , and hepa vacuum sock samples on nonporous surfaces indicated good agreement between results with hepa vacuum and wipe samples . 
 however , results from hepa vacuum sock and wipe samples agreed poorly with the swab samples . 
 dry swabs failed to detect spores > 75% of the time they were detected by wipe and hepa vacuum samples . 
 wipe samples collected after hepa vacuum samples and hepa vacuum samples after wipe samples indicated that neither method completely removed spores from the sampled surfaces . 
Methods
Results
Discussion