people with increased levels of crp are on higher risk to develop obesity , diabetes , cardiovascular diseases , and hypertension .
it has been experienced that the smoker males had increased levels of serum crp as compared to nonsmokers .
it has been observed that people with altered blood pressure and obesity has affected vascular endothelial function ; it occurs due to hypomagnesaemia and increase in plasma levels of crp in cellular processes .
healthy smokers have more insulin resistance which is a characteristics of metabolic syndrome as compared to nonsmokers .
it is also seen that they have also increased glucose levels , decreased high density lipoproteins ( hdl ) cholesterol , and much more very low - density lipoproteins ( vldl ) cholesterol .
since these variables are not direct measures of magnesium levels in smokers even then they clearly show that smoking decreases cellular magnesium concentration .
the purpose of present study was to find out the inverse relation between serum levels of crp and magnesium in smokers .
systemic acute phase reaction occurs due to inflammation , different studies calculated the level of circulating crp which shows the severity of inflammation .
the major initiator , interlukin-6 , released by hepatocyte increases the synthesis of crp in acute phase reactants .
in this case - control study , we took 192 healthy subjects including 96 smokers and 96 nonsmokers ( age range 20 - 40 years ) .
a written consent was obtained from every subject who was involved in our study before taking blood sample .
the serum levels for crp and magnesium were determined by standard methods using nycocard and diasys kits , respectively .
we included all the patients , who were smokers but healthy , having no any associated diseases like chronic obstructive pulmonary disease ( copd ) , obesity , angina , or myocardial infarction .
spirometry was not done , and we have ruled out any pathology concerned with lungs by history and symptoms , like they have no cough , tuberculosis ( tb ) , or any associated diseases .
statistical analysis was restricted by the number of cases over a period of 18 months , but sample size was not determined .
comparisons obtained in present study were presented as mean  standard deviation ( sd ) .
comparisons of mean values between smokers and nonsmokers were done by using student 's t - test .
statistical analysis was restricted by the number of cases over a period of 18 months , but sample size was not determined .
comparisons obtained in present study were presented as mean  standard deviation ( sd ) .
comparisons of mean values between smokers and nonsmokers were done by using student 's t - test .
table 1 shows the inverse relationship between serum crp and magnesium levels in smokers and nonsmokers .
the data shows that the mean serum crp concentration in smokers ( 14.62  0.16 mg
/ l ) was significantly higher ( p < 0.001 ) [ figures 1 and 2 ] .
comparison of serum c - reactive protein and magnesium levels between smokers and nonsmokers correlation between serum c - reactive protein ( crp ) and magnesium ( mg ) in smokers correlation between serum c - reactive protein ( crp ) and magnesium in nonsmokers similarly , the mean serum magnesium concentration in nonsmokers ( 2.52  0.18 mg
/ l ) was significantly higher ( p < 0.001 ) . surprisingly , serum magnesium levels in all smokers were found to be less than 1.8 mg / dl , while serum crp levels higher than 10 mg / l . on the contrary ,
all nonsmokers had their serum magnesium concentration greater than 2.0 mg / dl and serum crp concentrations less than 7.5 mg / l .
the crp to magnesium ratio in serum samples of smokers ranged between 0.73 and 5.0 as against 0.12 and 0.29 in nonsmokers , and it was significantly higher ( p < 0.001 ) in smokers as compared to nonsmokers [ table 2 ] .
this difference in the range of ratio clearly shows that decreased serum magnesium levels are independently related to high crp levels in smokers [ figure 3 ] .
comparison for c - reactive protein ( crp ) to magnesium ( mg ) ratio between smokers and nonsmokers correlation between mean levels of serum c - reactive protein ( crp ) and magnesium ( mg ) in smokers vs nonsmokers table 3 shows the analysis of two variables , that is , mg and crp are highly negatively related with each other , which means that when one variable increases other will decrease and vice versa , the analysis also shows the strength of relation between two variables .
the analysis also shows the strength of the relation between two variables . in this table ,
the strength is ( 0.764 ) , which shows that both variables are strongly negatively related and the results are significant at p < 0.001 .
pearson correlation between serum c - reactive protein ( crp ) and magnesium levels in smokers and nonsmokers table 4 shows the normal range of body mass index ( bmi ) in both cases and control ; hence , there is no obesity seen in both the groups.table 4comparison of age and body mass index ( bmi ) between healthy adult male smokers and nonsmokers 
  comparison of age and body mass index ( bmi ) between healthy adult male smokers and nonsmokers
in our study we have found that there is significantly increased level of serum crp , which is supported by das .
, observed the early outcomes of magnesium deficiency in rats during inflammatory response ; similarly , the present study also shows the deficiency of magnesium in inflammatory response .
the finding of the present study that smokers have increased level of crp with decreased level of magnesium is fully supported by khand et al .
guerrero - romero et al . , found that activated state of immune cells is the result of hypomagnesemia in nondiabetic , nonhypertensive , obese subjects ; this also supports the present study .
, that individuals with low intake of magnesium were more likely to have increased crps .
the present study clearly demonstrated that smoking significantly decreases serum magnesium concentration and increases serum crp concentration resulting in an inverse relationship between serum crp and magnesium concentrations in smokers only .
, that magnesium intake is inversely related with the crps in plasma for the prevalence of metabolic syndrome . according to another study of mendenhall et al .
, smoking , cardiovascular disease , and bmi have a great association with crps and we also found similar findings in our results with smoking .
, justified our study in way that crp have an association with the bmi , cardiovascular diseases , and smoking ; this study favors our results , but does not explain the inverse relationship between magnesium and crps .
 background : smoking plays a key role in increasing the inflammatory marker c - reactive protein ( crp).aims : to examine inverse correlation between crp and magnesium levels in smokers and nonsmokers.materials and methods : a total of 192 healthy adult male subjects were included in the present study , out of which 96 were smokers and the remaining 96 were nonsmokers having age range from 20 to 40 years , and all the subjects belonged to district matyari of hyderabad . 
 serum crp was measured by nycocard standard kit method and magnesium levels by diasys standard kit method in smokers and nonsmokers.results:the levels of serum crp in smokers ( 14.62  0.16 mg 
 / l ) is high as compared to nonsmokers ( 4.81  0.38 mg 
 / l ) , which is highly significant ( p < 0.001 ) . 
 however , inverse results were seen for serum magnesium levels which were significantly higher ( p < 0.001 ) in nonsmokers ( 2.52  0.18 mg 
 / l ) as compared to the smokers ( 1.09  0.38 mg / dl ) . a significant ( p < 0.001 ) inverse relationship between serum crp and magnesium concentrations 
 were seen in smokers.conclusion:this result shows that smoking increases serum crp , an inflammatory marker parallel to decrease in serum magnesium levels in smokers having 20 - 40 years of age . 
Introduction
Materials and Methods
Statistical analysis
Results
Discussion