they have more conservative cavity designs which basically rely on the effectiveness of current dentin bonding agents . however , composite resins still have some limitations in use due to their physical properties,[3 - 5 ] polymerization shrinkage , microleakage , low wear resistance and color stability,[7 - 8 ] and lower bond strength to dentin compared with enamel .
these properties are related to each other somehow ; polymerization shrinkage can cause composite debonding , leading to gap formation , microleakage , secondary caries and so on .
thus composite resin bond strength to dentin plays an important role in restoration success since the major surfaces of a preparation to be bonded are in dentin , in different depths , sometimes without any enamel margins .
although enamel and dentin are both highly hydrophilic , bonding mechanism seems to be different in each substrate because of basic differences in their organic and inorganic contents .
therefore , bonding to dentin presents a much greater challenge than to enamel , especially in deeper dentin due to a decrease in inter - tubular dentin and an increase in tubular diameter as well as tubular fluid.[13 - 14]efforts to improve clinical performance of methacrylate - based composite resins have led to the development of new monomers such as ring - opening silorane and new filler technology such as nanofillers .
filtek p90 ( p90 ) and filtek z350 ( z350 ) are two dental composite resins on the market that have lower polymerization shrinkage and improved mechanical properties , respectively when compared with other composite resins .
ring - opening polymerization technique in the silorane system , like p90 , is a recent important development in dental composite resins .
the term silorane comes from  siloxane  and  oxirane  , which combines their two advantages , i.e. high hydrophobicity from siloxane and ring - opening monomer from oxirane , to make silorane , with a self - etch and primer adhesive system .
the ring - opening process seems to be insensitive to oxygen - inhibiting action unlike methacrylate - based composite resins , which hinders polymerization by converting free radicals to stable species .
generally , the mechanical properties of composite resins are related to their filler load ; the more filler load in the composition the higher the mechanical properties such as wear resistance , modulus of elasticity and less polymerization shrinkage .
z350 is a recently introduced nanofilled composite resin with 6575 wt% of silica and zirconia nanofillers ,   which has been claimed to have a low shrinkage due to its high filler content . 
 
one strategy is to condition the dentin with disinfectants like ozone gas , oxalate desensitizer , naocl or edta and chx . in total etch adhesive systems ,
the adhesive may not reach the entire depth of demineralized dentin , thus using a penetration enhancer may improve adhesive penetration into demineralized dentin .
recently tjderhane et al . evaluated the effect of dentin pretreatment with dimethyl sulfoxide ( dmso : ( ch3)2so ) before application of the adhesive .
this compound is one of the best known penetration enhancers for medical purposes due to its dipolar amphiphilic nature and small size.the potential for tissue penetration and excellent solvent properties make dmso an attractive solvent candidate for the dental adhesives.the few studies on dmso available in dentistry are limited to its cryoprotection ability in preservation of pulpal and periodontal ligament undifferentiated cells . to the best of our knowledge
no studies have been carried out on deep and surface dentin pretreatment with dimethyl sulfoxide for the analysis of the shear bond strength .
the aim of this in vitro study was to evaluate the effect of surface and deep dentin pretreatment with dimethyl sulfoxide on shear bond strength ( sbs ) of a silorane - based and a methacrylate - based composite resin . 

in this experimental in vitro study , 80 recently extracted human maxillary first premolars , without any caries and cracks , stored in 0.2% sodium azide , were used .
the teeth were cleaned and mounted 2 mm below the cementoenamel junction in self - polymerizing acrylic resin ( acropars , iran ) to simulate tooth position in the alveolar bone .
the teeth were randomly divided into two groups ( n=40 ) , for sections of surface dentin ( group a ) or deep dentin ( group b ) .
the occlusal surfaces of the teeth were sectioned using diamond discs ( d&z ; germany ) under water cooling to remove the occlusal enamel and reach the central groove to expose the surface dentin just beneath the dentinoenamel junction in group a and 2 mm below the central groove to meet the deep dentin in group b as in previous studies .
the exposed dentin surface of all the specimens was ground with 600-grit abrasive paper to create a standardized smear layer .
then the specimens in each group were randomly assigned to 4 subgroups ( n=10 ) , according to the type of composite resins used ( two types ) and pre - treatment ( with or without pretreatment ) ( 
 figure 1 ) .  
composite resin build - up was done by using a 33-mm cylindrical translucent plastic mold , placed on the center of each specimen . a light - curing unit ( coltolux ii ; coltene , usa ) having a light output of 600 mw / cm was used to cure the adhesive systems and composite resins .
the light cure unit has been checked frequently after preparation of 5 teeth by using a radiometer ( cm 300 - 1000 ; halogen lightmeter , china ) .
materials , manufacturers and chemical compositions 
  statistically significant difference ( p < 0.05 ) .
the mean values followed by at least one similar letter were not significantly different . 

subgroup a1 : silorane adhesive system primer ( 3 m espe , usa ) was applied with a microbrush on dentin after shaking the bottle well , gently massaged for 15 s , dried with a gentle stream of air and light - cured for 10 s. then silorane adhesive system bond ( 3 m espe , usa ) was applied , followed by a gentle stream of air and light - cured for 10 s. filtek p90 composite resin ( 3 m espe , usa ) was placed in two increments of 1.5-mm thickness and each was light - cured for 40 s. 
 
 subgroup a2:acid phosphoric etchant gel ( etchant 37 ; denfil vericom co. ltd , korea ) was applied on dentin for 15 s , rinsed with water thoroughly for 10 s and dried for 35 s with a gentle stream of air , leaving the surface slightly moist .
two consecutive coats of adper single bond ( 3 m espe , usa ) were applied and light - cured for 10 s after gentle drying .
filtek z350 ( 3 m es pe , usa ) was placed in two increments measuring 1.5 mm in thickness and each was light - cured for 40 s. 
 subgroup a3:dentin was pretreated with 0.004% dimethyl sulfoxide ( merck , germany ) , which was obtained from diluting 3.55 ml dmso with distilled water to have 100 ml 0.004% dmso , for 30 s and gently dried , followed by the steps described for subgroup 1 . 

subgroup a4:etchant gel was applied on dentin for 15 s , rinsed thoroughly for 10 s and dried with a gentle stream of air , leaving the surface slightly moist .
dentin was pretreated with 0.004% dmso for 30 s and gently dried , followed by the bonding and restoration steps described for subgroup 2 . 

the teeth were stored in distilled water for 24 h at 37c to allow for water sorption and postoperative polymerization .
the teeth were then thermocycled ( pc300 ; vafaei , iran ) in distilled water at 52/552c for 1000 cycles with a dwell time of 30 s. there was a significant interaction effect between composite resin type and dentin depth ( p= 0.001 ) , composite resin type and dmso pretreatment ( p= 0.005 ) but not between dentin depth and dmso pretreatment ( p= 0.82 ) .
each specimen was individually subjected to a shear load applied by a wedge - shaped blade with a thickness of 1 mm in a universal testing machine ( zo20 ; zwick/ roell , 
 germany ) at a crosshead speed of 1 mm / min until failure occurred ( figure 2 ) .  
shear bond strength test 
  the force ( newton ) at failure was recorded and the shear bond strength values ( mpa ) were calculated from the peak load at failure divided by the bonded surface area in mm ( a= r , 3.141.51.5=7.065 mm ) . 
 
three - way analysis of variance ( anova ) and tamhane post hoc test were conducted by spss 15 .
in this experimental in vitro study , 80 recently extracted human maxillary first premolars , without any caries and cracks , stored in 0.2% sodium azide , were used .
the teeth were cleaned and mounted 2 mm below the cementoenamel junction in self - polymerizing acrylic resin ( acropars , iran ) to simulate tooth position in the alveolar bone .
the teeth were randomly divided into two groups ( n=40 ) , for sections of surface dentin ( group a ) or deep dentin ( group b ) .
the occlusal surfaces of the teeth were sectioned using diamond discs ( d&z ; germany ) under water cooling to remove the occlusal enamel and reach the central groove to expose the surface dentin just beneath the dentinoenamel junction in group a and 2 mm below the central groove to meet the deep dentin in group b as in previous studies .
the exposed dentin surface of all the specimens was ground with 600-grit abrasive paper to create a standardized smear layer .
then the specimens in each group were randomly assigned to 4 subgroups ( n=10 ) , according to the type of composite resins used ( two types ) and pre - treatment ( with or without pretreatment ) ( 
 figure 1 ) .  
composite resin build - up was done by using a 33-mm cylindrical translucent plastic mold , placed on the center of each specimen . a light - curing unit ( coltolux ii ; coltene , usa ) having a light output of 600 mw / cm was used to cure the adhesive systems and composite resins .
the light cure unit has been checked frequently after preparation of 5 teeth by using a radiometer ( cm 300 - 1000 ; halogen lightmeter , china ) .
materials , manufacturers and chemical compositions 
  statistically significant difference ( p < 0.05 ) .
the mean values followed by at least one similar letter were not significantly different . 

subgroup a1 : silorane adhesive system primer ( 3 m espe , usa ) was applied with a microbrush on dentin after shaking the bottle well , gently massaged for 15 s , dried with a gentle stream of air and light - cured for 10 s. then silorane adhesive system bond ( 3 m espe , usa ) was applied , followed by a gentle stream of air and light - cured for 10 s. filtek p90 composite resin ( 3 m espe , usa ) was placed in two increments of 1.5-mm thickness and each was light - cured for 40 s. 
 
 subgroup a2:acid phosphoric etchant gel ( etchant 37 ; denfil vericom co. ltd , korea ) was applied on dentin for 15 s , rinsed with water thoroughly for 10 s and dried for 35 s with a gentle stream of air , leaving the surface slightly moist .
two consecutive coats of adper single bond ( 3 m espe , usa ) were applied and light - cured for 10 s after gentle drying .
filtek z350 ( 3 m es pe , usa ) was placed in two increments measuring 1.5 mm in thickness and each was light - cured for 40 s. 
 subgroup a3:dentin was pretreated with 0.004% dimethyl sulfoxide ( merck , germany ) , which was obtained from diluting 3.55 ml dmso with distilled water to have 100 ml 0.004% dmso , for 30 s and gently dried , followed by the steps described for subgroup 1 . 

subgroup a4:etchant gel was applied on dentin for 15 s , rinsed thoroughly for 10 s and dried with a gentle stream of air , leaving the surface slightly moist .
dentin was pretreated with 0.004% dmso for 30 s and gently dried , followed by the bonding and restoration steps described for subgroup 2 . 

the teeth were stored in distilled water for 24 h at 37c to allow for water sorption and postoperative polymerization .
the teeth were then thermocycled ( pc300 ; vafaei , iran ) in distilled water at 52/552c for 1000 cycles with a dwell time of 30 s. there was a significant interaction effect between composite resin type and dentin depth ( p= 0.001 ) , composite resin type and dmso pretreatment ( p= 0.005 ) but not between dentin depth and dmso pretreatment ( p= 0.82 ) .
each specimen was individually subjected to a shear load applied by a wedge - shaped blade with a thickness of 1 mm in a universal testing machine ( zo20 ; zwick/ roell , 
 germany ) at a crosshead speed of 1 mm / min until failure occurred ( figure 2 ) .  
shear bond strength test 
  the force ( newton ) at failure was recorded and the shear bond strength values ( mpa ) were calculated from the peak load at failure divided by the bonded surface area in mm ( a= r , 3.141.51.5=7.065 mm ) . 

three - way analysis of variance ( anova ) and tamhane post hoc test were conducted by spss 15 .
the mean values and standard deviations of shear bond strengths are summarized in table 2 .   the mean values and 
 standard deviations of sbs statistically significant difference ( p < 0.05 )
pretreatment with dmso increased sbs in subgroups a3 , a4 and b4 in comparison with subgroups a1 , a2 and b2 , respectively , and the increase was statistically significant in subgroups a3 ( p= 0.003 ) and b4 ( p < 0.001 ) but not significant in subgroup a4 ( p= 0.963 ) .
dmso did not affect sbs of b3 comparing with b1 ( p= 1.00 ) . 
 
asb exhibited significantly higher sbs values in subgroups a2 ( p < 0.001 ) , a4 ( p= 0.002 ) and b4 ( p < 0.001 ) than in subgroups a1 , a3 and b3 , respectively .
subgroup b2 had higher sbs than subgroup b1 , but the difference was not significant ( p= 0.09 ) . 
 
sbs was higher in all the surface subgroups compared with deep subgroups ; it was significantly different in subgroup a2 compared with subgroup b2 ( p < 0.001 ) and in subgroup a3 compared with subgroup b3 ( p < 0.001 ) but not statistically significant in subgroup a1 compared with subgroup b1 ( p= 0.99 ) and in subgroup a4 compared with subgroup b4 ( p= 0.14 ) 

dentin pretreatment with dmso had a significant effect on sbs of asb to either surface or deep dentin . 
 
one is to condition dentin surface with chemicals such 
 as polyacrylic acid , chlorhexidine digluconate 
 and antibacterial agents such as edta and naocl .
evaluated the effect of 0.004% dmso on nanoleakage and microtensile bond strength of filtek supreme xt to dentin . a methacrylate - based ( z350 ) and a silorane - based 
 ( p90 ) composite resin were used in this study with their respective adhesive systems : a total - etch system , adper single bond ( asb ) , and a self - etch system , p90 
 adhesive bond .
we used 0.004% dmso to condition the surface and deep dentin and evaluate its effect on bond strength of sa and asb . in this study dmso increased 
 sbs in the surface dmso - treated sa subgroup , a3 , and deep dmso - treated asb subgroup , b4 , compared with respective control subgroups a1 and b2 , consistent with 
 a previous study that reported dmso can improve the resindentin bond . in surface
dmso - treated asb subgroup , 
 a4 , sbs was higher than its control subgroup b2 , and lower in deep dmso - treated subgroup b3 than its control subgroup b1 , but the differences were not 
 statistically significant .
dmso is a chemical penetration enhancing34 amphiphilic solvent , fully miscible in all the solvents used in adhesive dentistry . 
 
this agent is able to penetrate biological surfaces and compete 
 with water molecules in inter - peptide hydrogen bonding of the collagen matrix .
this may explain why conditioning 
 dentin surface with dmso before adhesive application improved adhesive penetration into the exposed collagen matrix .
the increase in sbs in the surface 
 dmso - treated asb subgroup was not as much as it was expected and since this is the first time that the effect of dmso on surface and deep dentin is studied , 
 we recommend more studies with more specimens and different concentrations of dmso and sem microscopy to understand its behavior on different depths of dentin . 

on the other hand , the adhesive system of silorane is a self - etch one , exclusively used with this composite resin . 
 [
36 - 38 ] the acidic hydrophilic primer with a ph of 2.7 is considered a relatively mild primer . 
 in the sa subgroups dmso
it seems that the dmso entrapped in large intra - tubular spaces of deep dentin 
 ( subgroup b3 ) has a tendency to dilute the primer and weaken its already mild demineralization capacity , leading to lower sbs in 
 deep dmso - conditioned p90 specimens ( subgroup b3 ) than non - conditioned one ( subgroup b1 ) .
in contrast , comparison of subgroup b4 
 and b2 did not show this compromising effect since dmso was applied after acid - etching in asb subgroups , as described in a previous 
 study and this can be the reason why sbs was higher in the asb subgroups compared to the 
 sa subgroups .
changes in the steps in which dmso is applied , may lead to different results . 
 
different bond strength results have been 
 reported with the use of total - etch and self - etch adhesives , with higher bond strength for either total - etch 
 or self - etch adhesives . in the present study
, asb subgroups were restored using a 
 total - etch adhesive system with 37% phosphoric acid , a strong acid ( ph=0.030.05 ) and 
 the application of an adhesive , asb .
this technique leads to a complete removal of the smear layer , demineralization of the dentin surface 
 and exposure of collagen fibers,31 creating a demineralized zone measuring 5 to 8m in thickness 
 but sa system s acidic hydrophilic primer with a ph of 2.7 leads to dentin decalcification limited to a few hundredths of nanometers . 
 
after the primer is light - cured , a more viscous and hydrophobic adhesive resin , must be 
 applied and light - activated independently.[43 - 45 ] the 
 larger quantity and depth of tags obtained with total - etch systems may promote deeper micromechanical interlocking and greater sbs compared 
 to self - etch systems like silorane.[44 - 46 ] all the deep dentin subgroups presented lower sbs than the surface dentin subgroups , consistent with previous studies . 
 
however , the difference was not significant in two pairs ; first in the surface 
 and deep sa subgroups without dmso which might be because of lower etching potential in silorane primer that can not demineralize 
 the highly mineralized components of the surface dentin in comparison with deep dentin ; and second in surface and deep dmso - treated asb 
 subgroups due to high demineralizing potential of phosphoric acid utilized in asb subgroups .
therefore , it seems that dmso pretreatment is 
 not as much effective on surface dentin as that on deep dentin when using asb .
self - etch adhesives have a mild acid primer that partially 
 demineralizes the dentin , despite of a total etch system that demineralizes the dentin to a higher extent and this can be the reason why we 
 had higher sbs values in b2 than in b1 although the difference was not significant . 
  due to insufficient data base on the mechanism by which dmso affects different depths of dentin and dentin bond strength , further studies are 
 required to evaluate its behavior in contact with acetone- , ethanol- or water - based dentin bonding agents and in different steps of a bonded 
 restorative procedure . furthermore , sem and tem analysis are recommended for better investigation of the depth of resin penetration with or without dmso . 

within the limitations of this in vitro study , pretreatment of dentin with dmso significantly improved sbs 
 of asb in comparison with sa .
dmso appeared to enhance sbs of asb to either surface or deep dentin and the 
 increase was much higher in deep dentin than in surface dentin .
therefore , when using asb , dmso might improve 
 sbs in deep cavities with no enamel margins if the results of this study are confirmed by in vivo studies . 

in addition , dmso could not affect sbs values in deep dentin restored with sa compared with surface dentin . 

 statement of the problem : composite bond to dentin is crucial in many clinical conditions particularly in deep cavities without enamel margins due to insufficient penetration of adhesive into demineralized dentin . 
 
 purpose : the aim of this study was to assess the shear bond strength ( sbs ) of a methacrylate - based and a silorane - based composite resin to surface and deep dentin after pretreatment with dimethyl sulfoxide ( dmso ) . 
 
 materials and method : eighty extracted human premolars were randomly divided into two groups of flat occlusal dentin with different cuts as a : surface group 
 ( sections just below the dentinoenamel junction ( dej ) and b : deep group ( 2 mm below dej ) . 
 each group was randomly assigned to 4 subgroups 
 and their samples were restored with adper single bond ( asb ) and filtek z350 or silorane system adhesive ( sa ) and filtek p90 composite resins , 
 using a 33 mm cylindrical plastic mold . following these steps , 
 the subgroups were assigned as subgroupa1 : surface dentin+ silorane system 
 primer ( ssp)+ silorane system bonding ( ssb)+ p90 ; subgroup a2 : surface dentin+ 37% etchant ( e37% ) + adper single bond ( asb)+ z350 ; 
 subgroup a3 : surface dentin+ dmso+ ssp+ ssb+ p90 ; subgroup a4 : surface dentin+ e37%+ dmso+ asb+ z350 ; subgroup b1 : deep dentin+ ssp+ ssb+ p90 ; 
 subgroup b2 : deep dentin+ e37%+ asb+ z350 ; subgroup b3 : deep dentin+ dmso+ ssp+ ssb+ p90 ; subgroup b4:dentin + e37% + dmso + asb + z350 . 
 the specimens 
 were thermocycled at 5 2/55 2c for 1000 cycles and then tested for sbs . 
 
 results : using dmso as dentin conditioner increased sbs of asb to deep dentin ( p < 0.001 ) and sbs of sa to surface dentin ( p= 0.003 ) but had no effect on sbs of sa to deep dentin ( p= 1.00 ) . 
 
 conclusion : the ability of dmso to increase sbs of asb to deep dentin provides a basis for improving bonding of this composite resin in deep cavities . 
 
Introduction
Materials and Method
Preparation of specimens
Shear bond strength analysis
Statistical analysis
Results
Discussion
Conclusion