advanced chronic periodontitis often coexists with poorly controlled diabetes , such that diabetes is considered to be a risk factor for periodontitis .
many studies conducted among adults have reported a significant positive two - way association between diabetes ( both type-1 and type-2 ) and periodontal disease that is , diabetes increases risk of developing periodontitis and worsens preexisting periodontitis and periodontitis may raise the risk of diabetes .
periodontal infection can increase systemic inflammation , which in turn may induce a chronic state of insulin resistance , contributing to the cycle of hyperglycemia and advanced glycation end product - protein binding accumulation .
therefore , it can amplify the classical pathway of connective tissue degradation , destruction and proliferation in diabetes .
moreover , studies have also shown that treating periodontitis in diabetic patients has a beneficial effect on their blood glucose control .
on the other hand , poorly controlled diabetes is an important risk factor for periodontitis , and gingivitis , and sometimes periodontitis is the first sign that a diabetic patient presents with .
the impact of periodontitis on the diabetes - related inflammatory status has also been studied .
it has been indicated that the cytokine  induced inflammatory state in periodontitis can contribute to the overall low  grade inflammation that occurs in diabetes .
a recent study investigated the relationship between chronic periodontitis , impaired fasting glucose ( ifg ) , and diabetes in the us population and found chronic periodontitis to be positively associated in a linear relation with ifg and diabetes in us adults .
studies evaluating the relationship between impaired glucose tolerance ( igt ) and periodontal disease among japanese population suggest that periodontal disease is positively associated with igt , but other studies found no such association .
obesity and impaired lipid profile are other strong risk factors for type-2 diabetes , which in turn is a risk factor for periodontal disease .
the extent of coronary atherosclerosis is positively correlated with pro - atherogenic lipids , that is , total cholesterol , low - density lipoprotein ( ldl ) cholesterol and triglycerides ( tg ) , and negatively correlated with anti - atherogenic high density lipoprotein ( hdl ) cholesterol . the high prevalence of cardiovascular disease and periodontitis in individuals with diabetes may be attributed to an increased inflammatory response leading to atherosclerosis as compared to those without diabetes .
though there are many studies that have evaluated the interrelationship between the diabetic status of an individual with his / her periodontal status ; the authors however could not find studies / research reports that evaluated relationship between various biochemical parameters like lipid profile and oral glucose tolerance test ( ogtt ) with periodontal health / disease status , in both healthy and diabetic patients .
hence , this study was designed to investigate the association between chronic periodontitis and diabetes by taking dental plaque index ( dpi ) and community periodontal index ( cpi ) score as periodontal health / disease dependent variables and lipid profile and ogtt as biochemical parameters in healthy , diabetic and igt subjects .
this study began in 2008 in the department of biochemistry and the department of periodontics at baba jaswant singh dental college hospital and research institute , ludhiana with the subjects reporting to the opd of the department of periodontics .
all patients reporting to the department of periodontics were interviewed and questioned about their diabetic / lipid profile status .
any patient reporting a known self or family history of deranged lipid / diabetic profile was provisionally selected for this study . of these ,
a group of 120 patients who were willing to form a part of the study were finally selected and were sent for blood and periodontal examination .
the subjects undertaken also fulfilled the following criteria - community periodontal index ( cpi ) score of 2 or more [ table 1 ] and cpi loss of attachment score of 0 and above [ table 2 ] . community periodontal index cpi loss of attachment score subjects with community periodontal index loss of attachment score 0 underwent a periodontal examination to determine mean periodontal pocket depth and attachment loss . for the biochemical analyses , the reports of ogtt and lipid profile tests were analyzed as per who criteria for the diagnosis of diabetes that is , normal glucose tolerance ( ngt ; fasting and 2 h postchallenge plasma glucose levels < 110 mg / dl and < 140 mg / dl , respectively ) , diabetes ( fasting or 2 h postchallenge plasma glucose levels 126 mg / dl or 200 mg / dl , respectively ) and igt ( igt ; all others with some glucose tolerance impairment including impaired fasting glucose ( ifg ) , that is , with one of the two glucose tolerance levels between normal and diabetic values and the other below the diabetic level ) .
the samples of venous blood were taken in the fasting state for ogtt and lipid profile analysis .
immediately after , 75 g glucose dissolved in 300 ml water was given to be ingested in about 5 min and sample of blood was again collected at an interval of 30 min for a period of 3 h for glucose tolerance test ( gtt ) analysis .
data obtained was tabulated , compared and statistically analyzed using z - test to know the correlation between lipid profile test values , type 2 diabetes mellitus and periodontitis .
subjects with community periodontal index loss of attachment score 0 underwent a periodontal examination to determine mean periodontal pocket depth and attachment loss . for the biochemical analyses , the reports of ogtt and lipid profile tests were analyzed as per who criteria for the diagnosis of diabetes that is , normal glucose tolerance ( ngt ; fasting and 2 h postchallenge plasma glucose levels < 110 mg / dl and < 140 mg / dl , respectively ) , diabetes ( fasting or 2 h postchallenge plasma glucose levels 126 mg / dl or 200 mg / dl , respectively ) and igt ( igt ; all others with some glucose tolerance impairment including impaired fasting glucose ( ifg ) , that is , with one of the two glucose tolerance levels between normal and diabetic values and the other below the diabetic level ) .
the samples of venous blood were taken in the fasting state for ogtt and lipid profile analysis .
immediately after , 75 g glucose dissolved in 300 ml water was given to be ingested in about 5 min and sample of blood was again collected at an interval of 30 min for a period of 3 h for glucose tolerance test ( gtt ) analysis .
data obtained was tabulated , compared and statistically analyzed using z - test to know the correlation between lipid profile test values , type 2 diabetes mellitus and periodontitis .
subjects with community periodontal index loss of attachment score 0 underwent a periodontal examination to determine mean periodontal pocket depth and attachment loss . for the biochemical analyses , the reports of ogtt and lipid profile tests were analyzed as per who criteria for the diagnosis of diabetes that is , normal glucose tolerance ( ngt ; fasting and 2 h postchallenge plasma glucose levels < 110 mg / dl and < 140 mg / dl , respectively ) , diabetes ( fasting or 2 h postchallenge plasma glucose levels 126 mg / dl or 200 mg / dl , respectively ) and igt ( igt ; all others with some glucose tolerance impairment including impaired fasting glucose ( ifg ) , that is , with one of the two glucose tolerance levels between normal and diabetic values and the other below the diabetic level ) .
the samples of venous blood were taken in the fasting state for ogtt and lipid profile analysis .
immediately after , 75 g glucose dissolved in 300 ml water was given to be ingested in about 5 min and sample of blood was again collected at an interval of 30 min for a period of 3 h for glucose tolerance test ( gtt ) analysis .
data obtained was tabulated , compared and statistically analyzed using z - test to know the correlation between lipid profile test values , type 2 diabetes mellitus and periodontitis .
of 120 subjects undertaken for study , 35 patients were found to be periodontally healthy and 85 periodontally diseased ; 48 subjects had igt , and 17 had diabetes and the rest of the subjects that is , 55 had ngt .
all subjects were assessed for their periodontal condition by taking dpi [ table 3 ] and cpi scoreas parameters . when dpi score ( suggesting periodontal condition of the patient ) and ogtt results were compared and studied , it was observed that dpi for ngt , igt and diabetic patients were 1.05  0.5 , 1.16  0.7 , and 1.65  0.8 , respectively [ table 4 ] . when cpi score ( suggesting periodontal condition of the patient ) and ogtt results were studied , it was observed that cpi score for ngt , igt , and diabetics were 1.33  0.4 , 1.67  0.5 , and 2.33  0.8 , respectively [ table 4 ] .
a similar correlation was found between ldl levels and ogtt results , that is , ldl levels in patients with ngt and igt were within normal range , while it was higher than normal in patients with diabetes [ table 5 ] .
mean total cholesterol and mean hdl values were within normal range for all ogtt patient types [ table 5 ] . in a correlation table of various biochemical analyses and periodontal parameters , [ table 6 ]
it is evident that out of 85 periodontally diseased patients 35 showed abnormal fasting blood sugar levels and 61 showed abnormal post prandial blood sugar levels which potrays a non - significant co - relation . of the 85 periodontally diseased patients 77 patients showed abnormal total serum cholesterol level , 65 showed abnormal ldl cholesterol level and 71 showed abnormal tg levels .
these findings suggest that worsening lipid profile test values can be positively correlated with increased severity of periodontal disease [ highly significant p < 0.001 ] .
dental plaque index ( sillness and loe , 1964 ) periodontal condition of subjects in relation to their biochemical status association of participants diabetic status ogtt and lipid profile tests correlation between periodontal condition and various biochemical parameters
the results of this study show that in patients having poor periodontal condition poor ogtt score was observed .
it is also clear that as the periodontal condition and ogtt scores worsen , the tg levels and ldl levels also worsen . it has been shown that diabetes is one of the predisposing factors for the development of periodontal disease .
the inter relationships between periodontitis and diabetes provide an example of systemic disease predisposing to oral infection , and once that infection is established , oral infection exacerbates the systemic disease .
it has been shown that diabetic patients are prone to elevated ldl cholesterol and tgs even when blood glucose levels were well controlled .
this is significant ; as our study indicated that hyperlipidemia may be one of the factors associated with periodontitis .
the results of the study suggest that periodontitis itself may lead to elevated ldl / tg levels . within this context
, it may be put forth that in addition to effects of diabetes , periodontitis may contribute to elevated serum lipids and potentially to systemic disease arising from chronic hyperlipidemia . in a recent study in women subjects ,
periodontal disease was shown to be associated with later development of impaired glucose metabolism with a prior history of gestational diabetes . therefore ,
treating periodontal disease may in addition to controlling and or improving the diabetic status of the patient may also improve the deranged lipid profile in a patient . when seen in this context , treating periodontal disease may also have a significant impact on improving the systemic health of the patient as both diabetes and a deranged lipid profile are known risk factors for several life - threatening diseases and conditions . as this study has demonstrated that the lipid profile can be a determinant of diabetes and periodontitis and vice versa
many cases of diabetes and deranged lipid profile may remain undiagnosed , and screening of patients in dental clinics may lead to a diagnosis of these in some cases .
it is evident from this study that abnormal levels of total serum cholesterol , ldl cholesterol , and tgs can be positively correlated with periodontally diseased conditions . as evidence of close link between periodontal disease , diabetes and deranged lipid profile
is seen , treating and preventing recurrence of periodontal disease in patients with diabetes and deranged lipid profile values should be considered an important component of the treatment and management of patients suffering from diabetes and deranged lipid profile .
 objective : a two - way relationship between diabetes and periodontal disease has been suggested ; whereas obesity and impaired lipid profile are risk factors for type-2 diabetes mellitus . 
 this study examined the relationship between lipid profile , oral glucose tolerance test ( ogtt ) with periodontal health / disease dependent variables in healthy , diabetic and impaired glucose tolerance subjects.materials and methods:120 patients were selected for the study and were determined to be periodontally healthy or diseased . 
 all these patients underwent biochemical tests for ogtt and lipid profile analysis and data was compared using z-test.results:the ogtt results deteriorated with deteriorating periodontal condition . a similar correlation 
 was also observed between worsening lipid profile test values , ogtt score , and periodontal condition.conclusion:this study indicates that hyperlipidemia may be one of the factors associated with periodontitis and that periodontitis may itself lead to abnormal serum lipid levels . 
 therefore , in addition to effects on diabetes , periodontitis may contribute to elevated serum lipid levels and therefore potentially to systemic disease arising from chronic hyperlipidemia . 
INTRODUCTION
MATERIALS AND METHODS
Criteria for selection of patients
Community periodontal index CPI 2
RESULTS
DISCUSSION
CONCLUSION