ankylosing spondylitis ( as ) , an inflammatory arthritis of the spine , is the prototype hla - b27-associated seronegative spondylarthritis.1 although several studies have examined the pathogenesis of as , the disease is not yet completely understood .
paraoxonase ( pon ) , an enzyme produced by the liver , is associated with high - density lipoprotein particles and is known to have an antioxidant function.24 in humans , the pon gene family has three members ( pon1 , pon2 , and pon3 ) that are located on chromosome 7q21.322.1.5 the physiological roles and natural substrates of these enzymes are not well known.6 pon1 has three known enzymatic molecules , including pon , arylesterase ( are ) , and dyazoxonase . pon1 hydrolyzes organophosphates such as paraoxon , hydrolyzes aromatic esters such as phenylacetate , and also decreases the accumulation of lipid peroxidation products.7 are , a thiol enzyme , is reactivated by 2-mercaptoethanol and cysteine but not by reduced glutathione .
are acts on phenyl acetate to release phenol , which can produce a stable indophenol dye with 4-aminoantipyrine and potassium ferricyanide.8 human serum pon1 and are are both esterase enzymes that have lipophilic antioxidant characteristics.9 these enzymes play a role in decreasing oxidative stress .
pon1 in particular is an important endogenous free radical scavenging system in the human body.10 serum pon1 acts in conjunction with are to function as a single enzyme.6,11 recently , a number of studies investigated serum pon and are activities in response to inflammatory arthritic conditions such as rheumatoid arthritis ( ra ) , psoriasis , and systemic lupus erythematosus ( sle ) with conflicting results.2,1217 to the best of our knowledge , serum pon and are activities have not previously been assessed in patients with as .
therefore , the aim of the present study was to assess whether pon and are levels may be related to disease activity and functional capacity in as patients .
thirty - two patients with as and 25 sex- and age - matched healthy controls were enrolled in the study .
the diagnosis of as was made according to the modified new york criteria.18 patients with as consumed non - steroidal anti - inflammatory drugs , but no tnf- inhibitory drugs , during this study .
none of the participants in the present study was obese , nor were any of them taking lipid lowering agents or antioxidant drugs .
we used the bath ankylosing spondylitis disease activity index ( basdai ) and the bath ankylosing spondylitis functional index ( basfi ) questionnaires for clinical assessment of the patients with as .
these two self - limited questionnaires are the gold standard for measuring disease activity and functional capacity in as patients .
the basdai consists of a 1 through 10 scale ( one indicating no problem and 10 indicating the worst problem ) that is used to answer six questions pertaining to the five major symptoms of as ( fatigue , spinal pain , joint pain , and enthesitis , as well as morning stiffness duration and severity).19 the basfi also has a 1 through 10 scale ( 1 indicating easy and 10 indicating impossible ) and consists of ten questions , eight of which relate to the functional capacity of patients and two of which relate to the patient s ability to cope with everyday life.20 this study was performed in accordance with the ethical standards set by the declaration of helsinki and was approved by the local ethics committee .
all participants were analyzed in terms of complete blood count and erythrocyte sedimentation rate ( esr ) , as well as c - reactive protein ( crp ) , serum pon , and are levels .
the serum was then separated from blood cells by centrifugation and immediately stored at 80c until analysis .
a nephelometric method was used for measuring crp levels ( beckman array protein system , usa ) .
assays were made without additional nacl ( baseline activity ) and with 1 mol / l nacl included in the assay buffer ( salt - stimulated activity ) , by following the formation of p - nitrophenol ( assessed by the absorbance at 405 nm ) for 5 min .
the assay buffer contained 0.125 mol / l tris - hcl ( ph 8.5 ) , 1.25 mmol / l cacl2 , and , for each set of assays , 6 mmol / l of a freshly prepared paraoxon ( o , o - diethyl-0-p - nitrophenylphosphate ; sigma chemical co ) substrate solution and 1 mol / l nacl ( ph 8.5 ) .
the assay tube contained 750 l of tris buffer , 50 l of serum ( diluted 1:2 with water ) , and 200 l of 6 mmol / l paraoxon .
the enzymatic reaction was started at 37c by the addition of the substrate solution . using a techcomp 8500 ii spectrophotometer ,
one unit of pon1 activity was defined as the enzyme quantity that disintegrated 1 mol of paraoxon substrate in 1 minute.21,22 the percent stimulation of pon1 was calculated as follows : [ ( pon1 activity with 1 mol / l nacl)-(basal activity)/basal pon1 activity ] x100.23 are activity was measured using phenylacetate as the substrate .
the assay tube contained 750 l of 0.1 mol / l tris - hcl at ph 8.5 , 1 mmol / l cacl2 , 125 l of 12 mmol / l phenylacetate , and 125 l of serum ( diluted 1:10 with water ) .
all participants were analyzed in terms of complete blood count and erythrocyte sedimentation rate ( esr ) , as well as c - reactive protein ( crp ) , serum pon , and are levels .
the serum was then separated from blood cells by centrifugation and immediately stored at 80c until analysis .
a nephelometric method was used for measuring crp levels ( beckman array protein system , usa ) .
assays were made without additional nacl ( baseline activity ) and with 1 mol / l nacl included in the assay buffer ( salt - stimulated activity ) , by following the formation of p - nitrophenol ( assessed by the absorbance at 405 nm ) for 5 min .
the assay buffer contained 0.125 mol / l tris - hcl ( ph 8.5 ) , 1.25 mmol / l cacl2 , and , for each set of assays , 6 mmol / l of a freshly prepared paraoxon ( o , o - diethyl-0-p - nitrophenylphosphate ; sigma chemical co ) substrate solution and 1 mol / l nacl ( ph 8.5 ) .
the assay tube contained 750 l of tris buffer , 50 l of serum ( diluted 1:2 with water ) , and 200 l of 6 mmol / l paraoxon .
the enzymatic reaction was started at 37c by the addition of the substrate solution . using a techcomp 8500 ii spectrophotometer ,
one unit of pon1 activity was defined as the enzyme quantity that disintegrated 1 mol of paraoxon substrate in 1 minute.21,22 the percent stimulation of pon1 was calculated as follows : [ ( pon1 activity with 1 mol / l nacl)-(basal activity)/basal pon1 activity ] x100.23 are activity was measured using phenylacetate as the substrate .
the assay tube contained 750 l of 0.1 mol / l tris - hcl at ph 8.5 , 1 mmol / l cacl2 , 125 l of 12 mmol / l phenylacetate , and 125 l of serum ( diluted 1:10 with water ) .
the as group was comprised of 32 patients ; nine were female and 23 male .
the mean age of these patients was 33 years ( range 2065 ) , and the disease duration was 8.13 years ( range 125 ) . in the control group ( n=25 ) , seven healthy volunteers were female and the others male .
there was no statistically significant difference between patients and controls with regard to demographic data ( p>0.05 ) ( table 1 ) . when laboratory findings were compared between the as and control groups , the esr but not crp levels differed significantly between the as and control group ( p<0.05 ) .
when compared to the healthy controls , there was no statistically significant difference in serum pon or are levels for patients with as ( p>0.05 ) ( table 1 ) .
in addition to these findings , we found no correlations among basdai and basfi scores , serum pon , or are values in the as group in the present study .
finally , there were no statistically significant correlations between serum pon and are levels or demographic variables ( e.g. , gender or disease duration ) .
the inflammation primarily affects the axial skeleton , peripheral joints , and extra - articular structures .
the pathophysiological basis of as has not yet been well identified , but immunological mechanisms have been implicated.24 various functions of neutrophils have been shown to be enhanced in as ; these stimulated neutrophil functions mediate oxidative stress , which may have an important role in the pathogenesis of as.2527 oxidative stress describes situations in which the production of damaging oxidants exceeds the organism s capacity to neutralize them.2628 a decisive role of oxidative stress in inflammatory diseases like as has not yet been fully elucidated . however , reactive oxygen species produced by activated neutrophils during inflammatory reactions play important functions in the pathogenesis of many inflammatory diseases.24,29 inflammatory reactions trigger oxidative stress that leads to increased oxidant levels , which in turn decrease the level of antioxidants .
the antioxidant defense system is responsible for protecting cells against the potentially harmful effect of oxidizing agents 30,31 in addition to these findings , a limited number of studies investigated the association between the pathogenesis of as and oxidative stress .
for example , ozgocmen et al.32 reported that oxidative stress and lipid peroxidation were accelerated in untreated patients with active as but found no significant correlation between oxidant / antioxidant levels and disease activity . in another study , karakoc and
coworkers24 indicated that increased oxidant and decreased antioxidant capacity might be related to the pathogenesis of as , but again no significant correlation between antioxidant / oxidant parameters and disease activity was found .
we found no statistically significant difference in antioxidant enzyme levels in as patients upon comparison to healthy controls and also detected no significant correlation between antioxidant parameters and disease activity .
however , various other reports in the medical literature have indicated alterations in serum pon and are activities for patients with different rheumatic diseases , such as ra , psoriasis , sle , and behcet s disease.2,1217 in many of these studies , pon and are activities were reported to be significantly lower in patients with rheumatic diseases than in healthy controls.2,1216 in another study , kiss and coworkers 16 indicated that are activity in patients was not different from that of controls , despite reduced pon activity in patients with sle .
in contrast , toker et al.17 reported that serum pon1 and are activities were significantly higher in patients with psoriasis than in controls .
thus , the range of findings remains complicated and controversial . to the best of our knowledge ,
the present study is the first to assess serum pon and are activities in patients with as .
we found no significant difference in serum pon and are activities in patients with as when compared to those of healthy controls .
in addition , there was no significant correlation among antioxidant parameters , disease activity , or the patient s functional ability .
serum pon and are activities may be within normal ranges and may not be associated with disease activity and functional capacity in patients with as . further research is needed to provide a deeper understanding of this disease .
 objectivesthe aim of this study was to investigate the activities of serum paraoxonase and arylesterase in patients with ankylosing spondylitis with respect to those of healthy controls , to assess whether these enzyme levels are related to disease activity and functional capacity.methodsthe study included 32 patients with ankylosing spondylitis whose diagnoses were made according to the modified new york criteria as well as 25 healthy controls matched for age and sex . 
 the bath ankylosing spondylitis disease activity index and the bath ankylosing spondylitis functional index were applied to the ankylosing spondylitis patients . as laboratory parameters , 
 the erythrocyte sedimentation rate and serum c - reactive protein level were measured in patients and control subjects . 
 paraoxonase and arylesterase enzyme activities were measured using appropriate methods.resultsno statistically significant differences ( p>0.05 ) were found between the ankylosing spondylitis patients and controls in terms of serum paraoxonase or arylesterase levels . furthermore , there was no correlation between clinical and laboratory parameters in patients with ankylosing spondylitis.conclusionserum paraoxonase and arylesterase levels in ankylosing spondylitis patients may not differ from those of healthy controls , and there is no significant correlation between antioxidant parameters and the bath ankylosing spondylitis disease activity index or bath ankylosing spondylitis functional index scores in ankylosing spondylitis patients . 
 further research is needed to provide deeper understanding of this disease . 
INTRODUCTION
MATERIALS AND METHODS
Laboratory assessments
Measurement of PON1 and ARE activities
Statistical analysis
RESULTS
DISCUSSION
CONCLUSIONS