obstructive sleep apnea ( osa ) is a common sleep disorder and is characterized by repeated episodes of partial ( hypopnea ) or complete ( apnea ) obstruction of the upper airway during sleep due to pharyngeal collapse , resulting in recurrent chronic intermittent hypoxia and fragmentation of sleep.1 population - based epidemiological studies indicate that osa affects at least 3%7% of middle - aged adult men , 2%5% of women , and up to 4% of children.26 osa may cause excessive daytime sleepiness , frequently altered cardiopulmonary function , independently increased risk for hypertension , insulin resistance , cardiovascular disease , and motor vehicle accidents.710 for patients , osa can result in deficits in executive function , memory , coordination of movement,11 and attention.12 although the main contributory factors are presumed to be sleep fragmentation and intermittent nocturnal hypoxemia during sleep apneas,13 the pathological mechanism of the brain deficits is still unclear . to date
, limited work has been done to investigate osa within a neuroscientific framework , and only a handful of studies have directly addressed the neural basis for the osa trait in patients .
previous neuroimaging studies have documented altered gray matter volume deficits in the hippocampus,1416 temporal lobe , frontal lobe , anterior cingulate cortex , and cerebellar regions17,18 in patients with osa using the voxel - based morphometry method . moreover ,
diffusion tensor imaging studies show a wide range of white matter integrity changes within the corpus callosum , frontal cortex , temporal cortex , parietal cortices , cingulum bundle , and cerebellum.1921 magnetic resonance spectroscopy is also a useful neuroimaging tool because it provides a measure of changes in cerebral metabolism that may reflect pathological insults to brain integrity .
several studies using magnetic resonance spectroscopy have demonstrated significant metabolic changes in osa.2224 although these studies have focused on structural and metabolic changes in the brains of patients with osa , little research has been undertaken to examine how the human brain is affected by osa , leaving the mechanisms unclear .
functional magnetic resonance imaging ( mri ) , a noninvasive , high spatial , and temporal resolution technique , can reflect the intrinsic functional organization of the human brain.25 previous functional mri studies of osa have mainly focused on task studies and more employ either respiratory or cognitive challenges , significant changes are found in brain activation levels compared with good sleepers ( gss).2628 in recent years , resting - state functional mri has become a hot topic in neuroimaging studies . regional homogeneity ( reho ) , a newly developed resting - state functional mri approach ,
can analyze the similarities and coherence of intraregional spontaneous low - frequency ( < 0.08 hz ) signal fluctuations using voxel - wise analysis across the whole brain .
reho is based on a data - driven approach , so does not require the onset time of the stimulus or prior knowledge , and has good test 
the simple calculations involved and reliable characterization make it a potentially useful tool for analysis of resting - state functional mri data , and reho could be regarded as a measure for investigating whether local synchronization of spontaneous brain activity is associated with osa trait .
reho has been used successfully to detect local abnormalities in subjects with various neurological diseases , including sleep deprivation,29 transient ischemic attacks,30 schizophrenia,31 parkinson s disease,32 and epilepsy.33 using reho , a significant pattern of abnormal local cortical and subcortical connectivity has been shown in patients with osa.34 however , resting - state functional mri data are still scarce in patients with the condition , so more research to reveal the neuropathophysiology consequences of osa would be of great significance . in this prospective study , we hypothesized that , compared with gss , patients with osa would exhibit abnormal reho activity in at least parts of the areas , based on the previous studies reviewed , including the prefrontal lobe , temporal lobe , hippocampus , and cerebellum . to test this hypothesis
, we used the reho method to investigate whether the synchrony of regional spontaneous brain activity on resting - state functional mri was altered in male patients with severe osa when compared with gss .
this comparison is important in osa studies because sleepiness and oxygen deficit increases in the supine position and is expected to be accentuated following osa .
twenty - five untreated male patients with severe osa and 25 male gss matched for age and years of education were recruited for this study from the sleep monitoring room in the respiratory department of the first affiliated hospital of nanchang university . each candidate had a detailed clinical interview and physical examination , completed a sleep questionnaire , and underwent overnight polysomnography .
inclusion criteria for the patients with osa were as follows : male gender ; age older than 22 years but younger than 60 years ; and an apnea - hypopnea index ( ahi ) > 30 .
exclusion criteria for both patients and gss were as follows : other sleep disorders , such as insomnia and a sleep - related eating disorder ; history of cardiovascular disease , hypothyroidism , or diabetes mellitus ; left - handedness ; alcohol or illicit drug abuse , or current intake of psychoactive medications ; a central nervous system disorder ( neurodegenerative disease , epilepsy , head injury , psychosis , current depression ) ; and contraindications to mri , such as claustrophobia , metallic implants , or devices in the body .
all subjects underwent a cognitive assessment using the montreal cognitive assessment administered by two independent neuropsychologists .
this study was approved by the human research ethics committee at the first affiliated hospital of nanchang university , and all participants provided their written informed consent .
we used standard overnight polysomnography with the le - series physiological monitoring system ( alice 5 le , respironics , orlando , fl , usa ) in the sleep center of our hospital , along with standard electroencephalogram , electrooculogram , chin electromyogram , and electrocardiogram checks simultaneously .
apnea was defined as continuous cessation of airflow for more than 10 seconds and hypopnea was defined as a decrease in airflow by more than 30% with arousal or oxygen desaturation more than 4%.35,36 the ahi was determined by dividing the average of the total number of apnea / hypopnea events by the estimated hours of sleep .
mri scanning was performed on a 3-tesla mr scanner ( siemens , munich , germany ) .
the scans consisted of a location image , t1-weighted sagittal images , t2wi axial / coronal images , and functional images . for the t1 sagittal images , 176 images were obtained ( repetition time 1,900 msec , echo time 2.26 msec , thickness 1.0 mm , gap 0.5 mm , acquisition matrix 256256 , field of view 250 mm 250 mm , flip angle 9 degrees ) . finally , at the same position , 240 functional images were obtained ( repetition time 2,000 msec , echo time 30 msec , thickness 4.0 mm , gap 1.2 mm , acquisition matrix 6464 , flip angle 90 degrees , field of view 230 mm 230 mm , 30 axial slices with gradient - recalled echo - planar imaging pulse sequence covering the whole brain ) .
the first ten time points for the functional images were discarded due to the possible instability of the initial mri signal and the participants adaptation to the scanning environment . on the basis of matlab2010a software ( mathworks , natick , ma , usa ) , the rest of the data preprocessing was performed using dparsfa software ( http://rfmri.org/dparsf ) , including dicom form transformation , slice timing , head motion correction , and spatial normalization .
motion time courses were obtained by estimating the values for translation ( mm ) and rotation ( degrees ) for each subject .
participants who had more than 1.5 mm maximum displacement in x , y , or z and 1.5 degrees of angular motion during whole functional mri scans were rejected .
the six friston head motion parameters was used to correct for the effects of head motion based on recent work showing that higher - order models are more effective in removing head motion effects.37,38 after correction for head motion , the functional mri images were spatially normalized to the montreal neurological institute space using the standard echo - planar imaging template in statistical parametric mapping 8 ( http://www.fil.ion.ucl.ac.uk/spm ) software and resampling the images at a resolution of 3 mm 3 mm 3 mm .
after preprocessing , the time series for each voxel were detrended and filtered ( bandpass 0.010.08 hz ) to reduce low - frequency drift and high frequency physiological respiratory and cardiac noise and time series linear detrending .
reho computation based on protocols from previous studies was performed with rest software ( http://www.resting-fmri.sourceforge.net).29 individual reho maps were generated by calculating kendall s coefficient of concordance ( kcc ) for the time series of a given voxel with those of its nearest neighbors ( 26 voxels ) in a voxel - wise analysis . the computational formula was39 

reho=(ri)2n(r)2k2(n3n)/12 , where reho is the kcc among the given voxels , ranging from 0 to 1 ; when a given cluster and its adjacent cluster in a time series is more consistent , the kcc value is more close to 1 .
k is the voxel number of time series within a measured cluster ( the smallest unit of measured reho , comprised of more adjacent clusters ) ; here , k = 27 ( one given voxel which was located in the cubic center plus its adjacent 26 voxels ) ; n is the number of ranks ; ri is the sum rank of the ith time point , where 
 r = ( n + 1)k/2 is the mean of the ri values ; the standard reho value is the reho value of each cluster / mean of the whole brain reho value ; thus , the individual reho map was generated for each dataset . to reduce the influence of individual variations in kcc value , normalization of the reho maps
was done by dividing the kcc for each voxel by the average kcc of the whole brain .
the resulting functional mri data were then spatially smoothed with a gaussian kernel of 666 mm full - width at half - maximum .
one - sample t - tests were first performed within each group to detect where the standardized kcc values were larger than the global mean kcc .
the between - conditions statistical threshold was set at a cluster size of p<0.001 , voxels at p<0.001 , and a cluster volume 1,080 mm ( a minimum continuous cluster volume of 1,080 mm ) , corrected by the false discovery rate .
group differences based on the reho method were investigated using a two - samples t - test in the rest tool kit .
the ages were included as nuisance covariates ( variables of no interest ) to remove the residual effect in the statistical models applied . a corrected significance level of individual voxel p<0.001 and cluster volume 1,080 mm , using a false discovery rate - corrected threshold of p<0.05 , was used to determine the between - conditions statistical significance .
the results were presented by xjview and rest . based on our reho findings , we identified brain regions that demonstrated significant between - group differences .
these regions were classified as regions of interest and saved as masks with rest software . for each region of interest ,
the mean reho value was extracted by averaging reho values over all voxels for each patient with osa .
finally , the mean reho values were entered into statistical package for the social sciences version 17.0 software ( spss inc , chicago , il , usa ) to calculate their correlation with behavioral performance .
twenty - five untreated male patients with severe osa and 25 male gss matched for age and years of education were recruited for this study from the sleep monitoring room in the respiratory department of the first affiliated hospital of nanchang university . each candidate had a detailed clinical interview and physical examination , completed a sleep questionnaire , and underwent overnight polysomnography .
inclusion criteria for the patients with osa were as follows : male gender ; age older than 22 years but younger than 60 years ; and an apnea - hypopnea index ( ahi ) > 30 .
exclusion criteria for both patients and gss were as follows : other sleep disorders , such as insomnia and a sleep - related eating disorder ; history of cardiovascular disease , hypothyroidism , or diabetes mellitus ; left - handedness ; alcohol or illicit drug abuse , or current intake of psychoactive medications ; a central nervous system disorder ( neurodegenerative disease , epilepsy , head injury , psychosis , current depression ) ; and contraindications to mri , such as claustrophobia , metallic implants , or devices in the body .
all subjects underwent a cognitive assessment using the montreal cognitive assessment administered by two independent neuropsychologists .
this study was approved by the human research ethics committee at the first affiliated hospital of nanchang university , and all participants provided their written informed consent .
we used standard overnight polysomnography with the le - series physiological monitoring system ( alice 5 le , respironics , orlando , fl , usa ) in the sleep center of our hospital , along with standard electroencephalogram , electrooculogram , chin electromyogram , and electrocardiogram checks simultaneously .
apnea was defined as continuous cessation of airflow for more than 10 seconds and hypopnea was defined as a decrease in airflow by more than 30% with arousal or oxygen desaturation more than 4%.35,36 the ahi was determined by dividing the average of the total number of apnea / hypopnea events by the estimated hours of sleep .
mri scanning was performed on a 3-tesla mr scanner ( siemens , munich , germany ) .
the scans consisted of a location image , t1-weighted sagittal images , t2wi axial / coronal images , and functional images . for the t1 sagittal images , 176 images were obtained ( repetition time 1,900 msec , echo time 2.26 msec , thickness 1.0 mm , gap 0.5 mm , acquisition matrix 256256 , field of view 250 mm 250 mm , flip angle 9 degrees ) . finally , at the same position , 240 functional images were obtained ( repetition time 2,000 msec , echo time 30 msec , thickness 4.0 mm , gap 1.2 mm , acquisition matrix 6464 , flip angle 90 degrees , field of view 230 mm 230 mm , 30 axial slices with gradient - recalled echo - planar imaging pulse sequence covering the whole brain ) .
the first ten time points for the functional images were discarded due to the possible instability of the initial mri signal and the participants adaptation to the scanning environment . on the basis of matlab2010a software ( mathworks , natick , ma , usa ) , the rest of the data preprocessing was performed using dparsfa software ( http://rfmri.org/dparsf ) , including dicom form transformation , slice timing , head motion correction , and spatial normalization .
motion time courses were obtained by estimating the values for translation ( mm ) and rotation ( degrees ) for each subject .
participants who had more than 1.5 mm maximum displacement in x , y , or z and 1.5 degrees of angular motion during whole functional mri scans were rejected .
the six friston head motion parameters was used to correct for the effects of head motion based on recent work showing that higher - order models are more effective in removing head motion effects.37,38 after correction for head motion , the functional mri images were spatially normalized to the montreal neurological institute space using the standard echo - planar imaging template in statistical parametric mapping 8 ( http://www.fil.ion.ucl.ac.uk/spm ) software and resampling the images at a resolution of 3 mm 3 mm 3 mm .
after preprocessing , the time series for each voxel were detrended and filtered ( bandpass 0.010.08 hz ) to reduce low - frequency drift and high frequency physiological respiratory and cardiac noise and time series linear detrending .
reho computation based on protocols from previous studies was performed with rest software ( http://www.resting-fmri.sourceforge.net).29 individual reho maps were generated by calculating kendall s coefficient of concordance ( kcc ) for the time series of a given voxel with those of its nearest neighbors ( 26 voxels ) in a voxel - wise analysis . the computational formula was39 

reho=(ri)2n(r)2k2(n3n)/12 , where reho is the kcc among the given voxels , ranging from 0 to 1 ; when a given cluster and its adjacent cluster in a time series is more consistent , the kcc value is more close to 1 .
k is the voxel number of time series within a measured cluster ( the smallest unit of measured reho , comprised of more adjacent clusters ) ; here , k = 27 ( one given voxel which was located in the cubic center plus its adjacent 26 voxels ) ; n is the number of ranks ; ri is the sum rank of the ith time point , where 
 r = ( n + 1)k/2 is the mean of the ri values ; the standard reho value is the reho value of each cluster / mean of the whole brain reho value ; thus , the individual reho map was generated for each dataset . to reduce the influence of individual variations in kcc value ,
normalization of the reho maps was done by dividing the kcc for each voxel by the average kcc of the whole brain .
the resulting functional mri data were then spatially smoothed with a gaussian kernel of 666 mm full - width at half - maximum .
one - sample t - tests were first performed within each group to detect where the standardized kcc values were larger than the global mean kcc . the between - conditions statistical threshold
was set at a cluster size of p<0.001 , voxels at p<0.001 , and a cluster volume 1,080 mm ( a minimum continuous cluster volume of 1,080 mm ) , corrected by the false discovery rate .
group differences based on the reho method were investigated using a two - samples t - test in the rest tool kit .
the ages were included as nuisance covariates ( variables of no interest ) to remove the residual effect in the statistical models applied . a corrected significance level of individual voxel p<0.001 and cluster volume 1,080 mm , using a false discovery rate - corrected threshold of p<0.05 , was used to determine the between - conditions statistical significance .
based on our reho findings , we identified brain regions that demonstrated significant between - group differences .
these regions were classified as regions of interest and saved as masks with rest software . for each region of interest ,
the mean reho value was extracted by averaging reho values over all voxels for each patient with osa .
finally , the mean reho values were entered into statistical package for the social sciences version 17.0 software ( spss inc , chicago , il , usa ) to calculate their correlation with behavioral performance .
the patients with osa had significant higher scores for body mass index ( t=6.25 , p<0.001 ) , ahi ( t=15.51 , p<0.001 ) , spo2 < 90% ( t=6.66 , p<0.001 ) , and epworth sleepiness scale ( t=7.64 , p<0.001 ) when compared with gss . compared with gss , patients with osa showed a significantly lower reho in the right medial frontal gyrus ( ba11 ) , the right superior frontal gyrus ( ba10 ) , the right cluster of the precuneus and angular gyrus ( ba39 ) , and the left superior parietal lobule ( ba7 ) , and a significantly higher reho in the right posterior lobe of the cerebellum , the right cingulate gyrus ( ba23 ) , and the bilateral cluster covering the lentiform nucleus , putamen , and insula ( ba13 ) . the details are presented in figure 1 and table 2 .
the mean reho value of these altered areas were extracted ( figure 2 ) . in patients with osa
, the ahi score showed a significant positive correlation with arousal index ( r=0.642 , p=0.001 ) and a negative correlation with percent time in rapid eye movement sleep ( r=0.429 , p=0.032 ) and montreal cognitive assessment score ( r=0.405 , p=0.045 ) .
percent time in stage 2 sleep showed a negative correlation with percent time in rapid eye movement sleep ( r=0.584 , p=0.002 ) and epworth sleepiness scale score ( r=0.531 , p=0.006 ) .
body mass index showed a significant positive correlation with arousal index ( r=0.582 , p=0.002 ) .
the observed higher mean reho signal values in the right posterior lobe of the cerebellum showed a significant positive correlation with percent time in stage 3 sleep ( r=0.458 , p=0.021 ) , the right cingulate gyrus showed a significant positive correlation with percent time in rapid eye movement sleep ( r=0.405 , p=0.045 ) , and a lower mean reho in the right cluster of the precuneus and angular gyrus showed a significant negative correlation with sleep time ( r=0.430 , p=0.032 ) .
the patients with osa had significant higher scores for body mass index ( t=6.25 , p<0.001 ) , ahi ( t=15.51 , p<0.001 ) , spo2 < 90% ( t=6.66 , p<0.001 ) , and epworth sleepiness scale ( t=7.64 , p<0.001 ) when compared with gss .
compared with gss , patients with osa showed a significantly lower reho in the right medial frontal gyrus ( ba11 ) , the right superior frontal gyrus ( ba10 ) , the right cluster of the precuneus and angular gyrus ( ba39 ) , and the left superior parietal lobule ( ba7 ) , and a significantly higher reho in the right posterior lobe of the cerebellum , the right cingulate gyrus ( ba23 ) , and the bilateral cluster covering the lentiform nucleus , putamen , and insula ( ba13 ) . the details are presented in figure 1 and table 2 .
in patients with osa , the ahi score showed a significant positive correlation with arousal index ( r=0.642 , p=0.001 ) and a negative correlation with percent time in rapid eye movement sleep ( r=0.429 , p=0.032 ) and montreal cognitive assessment score ( r=0.405 , p=0.045 ) .
percent time in stage 2 sleep showed a negative correlation with percent time in rapid eye movement sleep ( r=0.584 , p=0.002 ) and epworth sleepiness scale score ( r=0.531 , p=0.006 ) .
body mass index showed a significant positive correlation with arousal index ( r=0.582 , p=0.002 ) .
the observed higher mean reho signal values in the right posterior lobe of the cerebellum showed a significant positive correlation with percent time in stage 3 sleep ( r=0.458 , p=0.021 ) , the right cingulate gyrus showed a significant positive correlation with percent time in rapid eye movement sleep ( r=0.405 , p=0.045 ) , and a lower mean reho in the right cluster of the precuneus and angular gyrus showed a significant negative correlation with sleep time ( r=0.430 , p=0.032 ) .
our previous study using the reho method demonstrated that many brain areas show obvious sex differences , both during the rested wakeful condition and the sleep deprivation condition.29 to avoid the influence of sex differences , we chose only male patients with osa to participate in this research . to our knowledge , few resting - state functional mri studies have been conducted in patients with osa.34 the present study used reho to investigate the synchrony of regional spontaneous activity in resting - state functional mri among patients with osa .
consistent with our hypothesis , patients with osa showed significantly lower reho in the right medial frontal gyrus , right superior frontal gyrus , right cluster of the precuneus and right angular gyrus , and left superior parietal lobule , and higher reho in the right posterior lobe of the cerebellum , right cingulate gyrus , and bilateral cluster covering the lentiform nucleus , putamen , and insula compared with gss .
benedetti et al found that males had more right  left brain hemisphere differentiation than females.40 our previous sleep deprivation study found that both male and female subjects majorly represented strong unilateral between the good sleep condition and sleep deprivation condition , characterized by that altered reho areas are mainly represented in the left hemisphere , and few lower reho areas in the right hemisphere.29 from normal sleep status to sleep deprivation status , both male and female groups demonstrated a regular pattern of changes , ie , the unilateral tendency of reho regions in the brain started to disappear and these regions spread to the opposite side or bilaterally.29 in our study , the altered local coherence was mainly located in the right hemisphere , suggesting that male patients with osa had strong right  left brain hemisphere differentiation .
the most interesting finding of our study was the lower reho signals in the precuneus , angular gyrus , and medial frontal gyrus in patients with osa compared with gss .
to our knowledge , these areas have been identified as being part of a default mode network that is highly active during the resting state.41 these brain regions are thought to be involved in a group of high - level cognitive functions , variously described as visuospatial imagery , consciousness , episodic memory , executive cognitive control , and behavioral inhibition.42,43 previous studies showed decreased resting - state functional connectivity and gray matter volume in the medial prefrontal cortex of the anterior default mode network in patients with osa , indicating both structural and functional deficits in the medial prefrontal cortex which may be related to impaired cognitive function in these patients.44 moreover , intermittent hypoxia is found to have a negative correlation with cerebral activation in the bilateral frontal and left parietal regions , and may be a major factor in default mode network dysfunction in patients with osa.45 the precuneus is located in the posteromedial cortex of the parietal lobe and is a widespread network of higher association cortical and subcortical structures , and plays an important role in an array of advanced cognition functions , including visuospatial imagery , episodic memory retrieval , self - processing , and consciousness.42 a previous sleep study has shown abnormal brain function in this region.46 positron emission tomography measurements of glucose utilization indicated that metabolism was decreased in the precuneus and prefrontal cortex.47 the functional impairment of the precuneus may have been caused partly by remote effects originating from morphologically impaired areas with decreased connectivity.48 in support of these findings , we found that patients with osa had decreased reho in the precuneus compared with gss , and the altered reho in the precuneus showed a significant negative correlation with sleep time ( r=0.430 , p=0.032 ) , suggesting decreased sleep time may be an important factor for dysfunction in the precuneus .
therefore , analysis of reho may be useful for indexing the extent of sleep time and may be an early biomarker of brain injury in patients with osa .
joo et al found that patients with osa frequently showed dysregulation of respiratory control , which might be related to morphological differences in the gray matter areas of the brain , such as the superior frontal gyrus , cingulate gyrus , and left precentral gyrus.49 previous morphological mri studies showed significant gray matter loss or volume decrease in the frontal lobe,17,49 which may result in memory impairment , and executive dysfunctions .
a previous mrs study demonstrated significant lower n - acetylaspartate to creatine ratios in the frontal cortex and frontal white matter of patients with osa when compared with gss,22 suggesting the presence of cerebral damage , probably caused by chronic repeated intermittent hypoxia.23 previous task functional mri studies in individuals with osa showed decreased activation in the frontal cortex which was associated with both the duration of oxygen desaturation and the arousal index.50,51 in support of these findings , in our study we found that patients with osa showed a significantly altered pattern of local cortical and subcortical activity in the right frontal gyrus ( ba10 , ba11 ) when compared with gss .
we also found higher reho values in the right posterior lobe of the cerebellum , right cingulate gyrus , and bilateral cluster covering the lentiform nucleus , putamen , and insula in patients with osa compared with gss .
osa is characterized by repeated episodes of partial or complete obstruction of the upper airway during sleep.52 evidence has shown that during respiration some cingulate neurons discharged at pace , suggesting that the anterior cingulate cortex has a complex and indirect relationship to respiratory control mechanisms.53 moreover , it is important in the insular cortex for the perception of dyspnea.54 the insular and cingulate areas are activated by dyspnea.55,56 cerebellar structures have been associated with regulation of respiratory patterns.57 the lentiform nucleus is involved in advanced cognitive function , and is sensitive to intermittent hypoxia with abundant blood supply , because of which patients with osa , characterized by chronic intermittent hypoxia , showed higher reho in bilateral lentiform nucleus compared with gss , which may be a compensatory to adapt to exposed to long - term hypoxia condition .
interestingly , these areas displayed symmetrical distribution , which may contribute to the about equal degree of hypoxia between two hemispheres .
in conclusion , our results showed altered synchrony of regional spontaneous activity in the default mode circuit during the resting - state condition .
the reho on resting - state functional mri is a simple and noninvasive method for investigating osa , and is a promising tool for better understanding the neurobiological consequences of this condition .
although our results provide novel information with regard to our understanding of osa , several limitations need to be considered .
firstly , larger sample sizes and female groups are needed in future studies to explore the mechanisms of osa in the brain .
secondly , polysomnographic data were obtained after a single - night recording , so a first - night effect on sleep parameters could not been excluded .
thirdly , we only focused on the effect of severe osa ; other types of patients with osa were not examined in this study .
 backgroundprevious studies have demonstrated that obstructive sleep apnea ( osa ) is associated with abnormal brain structural deficits . 
 however , little is known about the changes in local synchronization of spontaneous activity in patients with osa . 
 the primary aim of the present study was to investigate spontaneous brain activity in patients with osa compared with good sleepers ( gss ) using regional homogeneity ( reho ) analysis based on resting - state functional magnetic resonance imaging ( mri).methodstwenty - five untreated male patients with severe osa and 25 male gss matched for age and years of education were included in this study . 
 the reho method was calculated to assess the strength of local signal synchrony and was compared between the two groups . 
 the observed mean reho values were entered into statistical package for the social sciences software to assess their correlation with behavioral performance.resultscompared with gss , patients with osa showed significantly lower reho in the right medial frontal gyrus ( ba11 ) , right superior frontal gyrus ( ba10 ) , right cluster of the precuneus and angular gyrus ( ba39 ) , and left superior parietal lobule ( ba7 ) , and higher reho in the right posterior lobe of the cerebellum , right cingulate gyrus ( ba23 ) , and bilateral cluster covering the lentiform nucleus , putamen , and insula ( ba13 ) . 
 the lower mean reho value in the right cluster of the precuneus and angular gyrus had a significant negative correlation with sleep time ( r=0.430 , p=0.032 ) , and higher reho in the right posterior lobe of the cerebellum showed a significant positive correlation with stage 3 sleep ( r=0.458 , p=0.021 ) and in the right cingulate gyrus showed a significant positive correlation with percent rapid eye movement sleep ( r=0.405 , p=0.045).conclusionpatients with osa showed significant regional spontaneous activity deficits in default mode network areas . 
 the reho method is a useful noninvasive imaging tool for detection of early changes in cerebral reho in patients with osa . 
Introduction
Materials and methods
Subjects
Polysomnography
MRI parameters
Functional MRI data analysis
Statistical analysis
Brain-behavior correlation analysis
Results
Demographic and clinical data
Changes in ReHo in OSA compared with GSs
Correlation analysis
Discussion
Conclusion