prevalence and risk of overweight were assessed in 232 children who were enrolled in the prospective german gdm offspring study between 1989 and 2000 .
all children were from singleton births , had a mother with gdm diagnosed according to criteria of the german diabetes association using an oral glucose tolerance test ( ogtt ) with a 75-g glucose load .
women were considered to have gdm if two of three capillary blood glucose values exceeded the following limits : > 5 mmol / l ( fasting ) before an oral glucose tolerance test , > 10.0 mmol / l after 60 min , and 8.6 mmol / l after 120 min .
prevalence of overweight was compared with that of a cohort of ot1d ( n = 757 ) and offspring of nondiabetic mothers ( ondm ) ( n = 431 ) followed in the babydiab study ( 6 ) . in both
the gdm and babydiab studies , mothers and their offspring were recruited between 1989 and 2000 germany - wide through the same network of collaborating obstetric departments and pediatricians and entered into the study before the offspring reached the age of 3 months .
all children were followed with similar follow - up visits until the age of 14 years . in both studies ,
> 98% of the families were caucasian . in families with more than one offspring participating in the study ,
data on weight and height were collected at birth and at age 2 , 8 , and 11 years by physicians . at birth ,
length was measured on a measuring board and weight on a calibrated scale by health care professionals .
weight and height were obtained for 215 children at age 2 years , 89 children at age 8 years , and 74 children at age 11 years .
birth weight was adjusted for sex and gestational age and is expressed as a percentile of the german reference population ( 8) .
offspring less than the 10th percentile were defined as small for gestational age , those 90th percentile as large for gestational age and those between > 10th and < 90th percentile as appropriate for gestational age . from age 2 years
, height and weight were measured by physicians at regular clinical visits using a standard protocol .
weight was measured on a calibrated scale with the subject wearing light clothing , and height was measured with a fixed tape standing barefoot .
height , weight , and bmi from age 2 years were expressed as percentiles adjusted for age and sex according to german reference data ( 9 ) .
overweight was defined as bmi percentile 90 . maternal height and weight in early pregnancy were collected by physicians at the first pregnancy visit , bmi was calculated and categorized as obese ( 30 kg / m ) , overweight ( 25.029.9 kg / m ) , or normal weight ( < 25 kg / m ) .
data on diabetes treatment ( categorized as treated by insulin injections or diet alone ) and maternal smoking behavior during pregnancy ( defined as < or 1 cigarette / day ) were obtained from a questionnaire at birth .
since 2003 , fasting blood samples were collected for the determination of insulin resistance in ogdm as well as in offspring from the babydiab study .
insulin resistance was measured by homeostasis model assessment of insulin resistance ( homa - ir ) .
a total of 751 children ( including 74 ogdm and 425 ot1d ) with data on homa - ir either at age 8 or 11 years were included in this substudy .
fasting insulin was determined centrally using an automated immunoassay analyzer ( aia 360 ; tosoh , san francisco , ca ) .
the interassay coefficient of variation of the insulin assay is 8.4% at a concentration of 22.6 u / ml and the lower limit of detection is 0.5 u / ml .
overweight prevalence in offspring at age 2 , 8 , and 11 years was compared between groups using the  test .
logistic regression analyses were performed to test the association of maternal bmi at early pregnancy , birth size , maternal smoking during pregnancy , treatment modality during pregnancy , and breast - feeding duration as univariate covariates with overweight risk in ogdm , giving the odds ratio ( or ) and 95% ci for overweight for each of the covariates .
factors that were significantly associated with overweight risk in the univariate analysis were included as covariates in the multivariate logistic regression analysis .
missing data were not considered in the univariate analyses but were included as a separate categorical value for each of the variables included in the multivariate analysis .
linear regression was used to compare homa - ir in ogdm with those in ot1d and in ondm adjusted for age and sex and to analyze factors associated with homa - ir in ogdm . for all analyses , two - tailed p <
all statistical analyses were performed using spss 17.0 ( spss , chicago , il ) .
data on weight and height were collected at birth and at age 2 , 8 , and 11 years by physicians . at birth ,
length was measured on a measuring board and weight on a calibrated scale by health care professionals .
weight and height were obtained for 215 children at age 2 years , 89 children at age 8 years , and 74 children at age 11 years .
birth weight was adjusted for sex and gestational age and is expressed as a percentile of the german reference population ( 8) .
offspring less than the 10th percentile were defined as small for gestational age , those 90th percentile as large for gestational age and those between > 10th and < 90th percentile as appropriate for gestational age . from age 2 years
, height and weight were measured by physicians at regular clinical visits using a standard protocol .
weight was measured on a calibrated scale with the subject wearing light clothing , and height was measured with a fixed tape standing barefoot .
height , weight , and bmi from age 2 years were expressed as percentiles adjusted for age and sex according to german reference data ( 9 ) .
maternal height and weight in early pregnancy were collected by physicians at the first pregnancy visit , bmi was calculated and categorized as obese ( 30 kg / m ) , overweight ( 25.029.9 kg / m ) , or normal weight ( < 25 kg / m ) .
data on diabetes treatment ( categorized as treated by insulin injections or diet alone ) and maternal smoking behavior during pregnancy ( defined as < or 1 cigarette / day ) were obtained from a questionnaire at birth . written informed consent
since 2003 , fasting blood samples were collected for the determination of insulin resistance in ogdm as well as in offspring from the babydiab study .
insulin resistance was measured by homeostasis model assessment of insulin resistance ( homa - ir ) .
a total of 751 children ( including 74 ogdm and 425 ot1d ) with data on homa - ir either at age 8 or 11 years were included in this substudy .
fasting insulin was determined centrally using an automated immunoassay analyzer ( aia 360 ; tosoh , san francisco , ca ) .
the interassay coefficient of variation of the insulin assay is 8.4% at a concentration of 22.6 u / ml and the lower limit of detection is 0.5 u / ml .
overweight prevalence in offspring at age 2 , 8 , and 11 years was compared between groups using the  test .
logistic regression analyses were performed to test the association of maternal bmi at early pregnancy , birth size , maternal smoking during pregnancy , treatment modality during pregnancy , and breast - feeding duration as univariate covariates with overweight risk in ogdm , giving the odds ratio ( or ) and 95% ci for overweight for each of the covariates .
factors that were significantly associated with overweight risk in the univariate analysis were included as covariates in the multivariate logistic regression analysis . missing data were not considered in the univariate analyses but were included as a separate categorical value for each of the variables included in the multivariate analysis .
linear regression was used to compare homa - ir in ogdm with those in ot1d and in ondm adjusted for age and sex and to analyze factors associated with homa - ir in ogdm . for all analyses , two - tailed p <
all statistical analyses were performed using spss 17.0 ( spss , chicago , il ) .
prevalence of overweight in ogdm was 17.2% at age 2 years , 20.2% at age 8 years , and 31.1% at age 11 years .
prevalence of overweight was increased in ogdm compared with ot1d ( 15.8 , 11.0 , and 15.8% at age 2 , 8 , and 11 years , respectively ; p < 0.05 , p = 0.03 , and p < 0.01 , respectively ) and with ondm ( 11.4 , 10.3 , and 15.5% ; p = 0.7 , p = 0.02 , and p = 0.005 , respectively ( fig .
1 ) . prevalence of overweight at 2 , 8 , and 11 years of age in ogdm (  ) , ot1d ( ) , and ondm (  ) . in ogdm ,
maternal obesity was a strong predictor of overweight in children at age 2 , 8 , and 11 years in the univariate and multivariate logistic regression model ( tables 1 and 2 ) .
the prevalence of overweight in children at 2 , 8 , and 11 years of age was 24.6 , 36.4 , and 45.8% in children of obese mothers compared with 9.2 , 11.3 , and 11.9% of children born to nonobese mothers ( p = 0.01 , p = 0.02 , and p = 0.003 , respectively ) ( fig .
birth size , therapy of gdm during pregnancy , and maternal smoking during pregnancy showed inconsistent associations with overweight risk in the offspring ( tables 1 and 2 ) .
associations of maternal bmi in early pregnancy , birth size , therapy modality , and smoking behavior during pregnancy on overweight - risk ( bmi  90th percentile ) in ogdm at 2 , 8 , and 11 years of age : univariate analysis aga , appropriate for gestational age ; lga , large for gestational age ; sga , small for gestational age .
associations of maternal obesity in early pregnancy , lga status , and maternal smoking during pregnancy on overweight risk in ogdm at 2 , 8 , and 11 years of age : multivariate analysis variables included in the multivariate analysis : maternal bmi before pregnancy , birth size , and maternal smoking during pregnancy .
prevalence of overweight at 2 , 8 , and 11 years of age in ogdm in relation to maternal bmi in early pregnancy : bmi < 25.0 kg / m (  ) , bmi 25.029.9 kg / m ( ) , and bmi 30 kg / m (  ) . in the substudy on insulin resistance ,
ogdm had increased homa - ir compared with that of children of ondm and of ot1d ( p = 0.04 and p = 0.03 , adjusted for age and sex ) ( fig .
homa - ir was associated with the child 's bmi ( p = 0.01 , adjusted for age and sex ) ( supplementary fig .
1a , available in an online appendix at http://care.diabetesjournals.org/cgi/content/full/dc10-0139/dc1 ) but not with maternal obesity in early pregnancy ( supplementary fig . 1b ) .
homa - ir ( mean  1 se ) at age 11 years in ogdm compared with ot1d and ondm .
prevalence of overweight in ogdm was 17.2% at age 2 years , 20.2% at age 8 years , and 31.1% at age 11 years .
prevalence of overweight was increased in ogdm compared with ot1d ( 15.8 , 11.0 , and 15.8% at age 2 , 8 , and 11 years , respectively ; p < 0.05 , p = 0.03 , and p < 0.01 , respectively ) and with ondm ( 11.4 , 10.3 , and 15.5% ; p = 0.7 , p = 0.02 , and p = 0.005 , respectively ( fig .
1 ) . prevalence of overweight at 2 , 8 , and 11 years of age in ogdm (  ) , ot1d ( ) , and ondm (  ) . in ogdm ,
maternal obesity was a strong predictor of overweight in children at age 2 , 8 , and 11 years in the univariate and multivariate logistic regression model ( tables 1 and 2 ) .
the prevalence of overweight in children at 2 , 8 , and 11 years of age was 24.6 , 36.4 , and 45.8% in children of obese mothers compared with 9.2 , 11.3 , and 11.9% of children born to nonobese mothers ( p = 0.01 , p = 0.02 , and p = 0.003 , respectively ) ( fig .
birth size , therapy of gdm during pregnancy , and maternal smoking during pregnancy showed inconsistent associations with overweight risk in the offspring ( tables 1 and 2 ) .
associations of maternal bmi in early pregnancy , birth size , therapy modality , and smoking behavior during pregnancy on overweight - risk ( bmi  90th percentile ) in ogdm at 2 , 8 , and 11 years of age : univariate analysis aga , appropriate for gestational age ; lga , large for gestational age ; sga , small for gestational age .
associations of maternal obesity in early pregnancy , lga status , and maternal smoking during pregnancy on overweight risk in ogdm at 2 , 8 , and 11 years of age : multivariate analysis variables included in the multivariate analysis : maternal bmi before pregnancy , birth size , and maternal smoking during pregnancy .
prevalence of overweight at 2 , 8 , and 11 years of age in ogdm in relation to maternal bmi in early pregnancy : bmi < 25.0 kg / m (  ) , bmi 25.029.9 kg / m ( ) , and bmi 30 kg / m (  ) .
in the substudy on insulin resistance , ogdm had increased homa - ir compared with that of children of ondm and of ot1d ( p = 0.04 and p = 0.03 , adjusted for age and sex ) ( fig .
homa - ir was associated with the child 's bmi ( p = 0.01 , adjusted for age and sex ) ( supplementary fig .
1a , available in an online appendix at http://care.diabetesjournals.org/cgi/content/full/dc10-0139/dc1 ) but not with maternal obesity in early pregnancy ( supplementary fig .
homa - ir ( mean  1 se ) at age 11 years in ogdm compared with ot1d and ondm .
this study shows an increased prevalence of overweight at 2 , 8 , and 11 years of age in ogdm compared with ot1d and ondm . in ogdm ,
overweight up to age 11 years was strongly associated with maternal obesity at early pregnancy .
birth size of the child , maternal smoking during pregnancy , or treatment modality of gdm were less consistent predictors of overweight in children of mothers with gdm .
this gdm offspring study is a prospective study from birth , in which questionnaires addressing in pregnancy - related factors were administered at birth and children 's weight and height were measured regularly by a pediatrician according to standard protocols .
recall bias and over- or underestimating of growth that may occur when data are reported by parents are therefore avoided or limited .
our results are based on the definition of overweight as bmi percentile 90 , which has been proposed by the german association of obesity during childhood and obesity .
findings were consistent when overweight was defined as bmi 85 or 95 percentile ( data not shown ) .
although not population based , this is the largest prospective study following caucasian children of mothers with gdm from birth in defined follow - up intervals , enabling a longitudinal analysis of factors affecting overweight risk .
nevertheless , the numbers of children of mothers with gdm remains relatively low at ages 8 and 11 years . as a consequence , cis for some of the risk estimates are wide , and some associations may change with a larger number of subjects .
a small number of studies have examined the impact of maternal diabetes on overweight risk in offspring , differentiating between ogdm and offspring of mothers with preexisting type 1 diabetes ( 10,11 ) .
clausen et al . ( 11 ) reported an increased prevalence of overweight in adults who were from gdm pregnancies ( 40% ) or type 1 diabetes pregnancies ( 41% ) compared with control subjects ( 24% ) .
( 10 ) found only a minor increase in the prevalence of overweight in ogdm children ( 30% ) compared with control children ( 23% ) and ot1d ( 23% ) .
the findings of lawlor et al . support our previous report that ot1d are not at higher risk for overweight compared with ondm ( 6 ) .
however , in the current study , we saw a pronounced difference between ogdm and ot1d , whereas differences are not significant in the study of lawlor et al . to reconcile this discrepancy
, it is noted that our study in germany reported a lower background prevalence of overweight in children compared with the u.s . and that the numbers of ot1d and ogdm are larger in our study .
our findings are unlikely to be biased with respect to the comparisons between ogdm and ot1d children because both cohorts were recruited over the same period and from the same region and ethnic background .
they are also supported by expected associations of maternal bmi with overweight risk in the ogdm .
however , data for potential confounders such as socioeconomic status , dietary habits during childhood , and physical activity were not collected , and we can not exclude the possibility that the associations observed could , in some cases , be due to one or more of these confounding variables .
the finding that overweight risk is significantly higher in ogdm compared with ot1d indicates that exposure to hyperglycemia during pregnancy per se can only partly explain the increased prevalence of overweight in these children .
maternal obesity in early pregnancy was the strongest predictor of overweight risk at 2 , 8 , and 11 years in ogdm .
others have shown that maternal pregravid bmi is the strongest predictor of childhood obesity independent of maternal glucose status or birth weight ( 7 ) .
it has also been shown that childhood bmi correlates more closely with maternal bmi than does paternal bmi , indicating that in addition to genetic influences an obese intrauterine environment per se may contribute to the higher overweight risk in children of obese mothers ( 12,13 ) .
this finding is strengthened by results from animal studies showing that feeding - induced obesity at conception increases the prevalence of obesity in offspring .
programming of obesity in animals was independent of changes in birth weight and was associated with significant changes in metabolic and endocrine parameters and adipose tissue cellularity independent of postnatal caloric intake ( 13 ) .
finally , the possibility that the influence of maternal obesity on childhood overweight could also be due to similar adverse dietary and physical activity behaviors in obese mothers and their offspring should be considered .
similarly , the observation that maternal smoking during pregnancy is associated with overweight in the child could further indicate an influence via an  unhealthy  postnatal environment .
the relationship between childhood obesity and both maternal obesity and smoking during pregnancy became more pronounced with age , further giving support to this hypothesis .
not unexpectedly given the increased risk for overweight in ogdm , our substudy found higher homa - ir in ogdm than in ondm and also in ot1d .
( 14 ) who also reported a relationship between offspring bmi and insulin resistance in a small group of 23 female ogdm .
a recently published study found that in offspring of mothers with pregravid obesity fetal insulin resistance strongly correlated with fetal adiposity ( 15 ) .
we could not find a significant association between insulin resistance in the offspring and maternal obesity in early pregnancy , but numbers were relatively small . in summary , our results show that prevalence of overweight and , as a consequence , homa - ir during childhood is higher in ogdm than in ot1d , indicating that maternal diabetes type affects overweight risk .
the finding that overweight risk was associated with maternal obesity and to a lesser extent with birth size suggests that a combination of genetic predisposition , fetal overnutrition and lifestyle factors are likely to contribute to childhood growth in ogdm .
 objectivegestational diabetes mellitus ( gdm ) is associated with high birth weight in the offspring . 
 this may lead to overweight and insulin resistance during childhood . 
 the aim of the study was to assess the impact of gdm on overweight risk and insulin resistance in offspring.research design and methodsbmi measurements were collected at age 2 , 8 , and 11 years from 232 offspring of mothers with gdm ( ogdm ) and compared with those from 757 offspring of mothers with type 1 diabetes ( ot1d ) and 431 offspring of nondiabetic mothers ( ondm ) born between 1989 and 2000 . 
 insulin resistance ( homeostasis model assessment of insulin resistance [ homa - ir ] ) was determined at age 8 and 11 years in 751 children ( 74 ogdm ) . 
 overweight was defined as bmi percentile 90 ; insulin resistance was defined by homa-ir.resultsoverweight prevalence was increased in ogdm compared with ot1d and to ondm throughout childhood ( age 11 years 31.1 , 15.8 , and 15.5% ; p = 0.005 ) . 
 maternal obesity was an important predictor of overweight risk in children ( age 11 years odds ratio 7.0 [ 95% ci 1.827.7 ] ; p = 0.006 ) ; birth size and maternal smoking during pregnancy were inconsistently associated with and treatment of gdm during pregnancy did not affect overweight risk . 
 homa - ir was increased in ogdm compared with offspring of ondm mothers ( p = 0.01 , adjusted for sex and age ) and was associated with the child 's bmi ( p = 0.004).conclusionsoverweight and insulin resistance in children is increased in ogdm compared with ot1d or ondm . 
 the finding that overweight risk is associated mainly with maternal obesity suggests that familial predisposition contributes to childhood growth in these offspring . 
RESEARCH DESIGN AND METHODS
Data collection
Insulin resistance measurements substudy
Statistical analysis
RESULTS
Prevalence and predictors of overweight in OGDM
Predictors of insulin resistance during childhood (substudy)
CONCLUSIONS