for intracellular pathogens whose proliferation requires nutritional supplies from the host cell cytosol , the death of the cell before the completion of a productive infection is disastrous .
furthermore , cells actively executing the apoptotic processes often secrete chemical signals or display specific molecules on their surface so that phagocytes can recognize and engulf them , leading to the termination of infection .
even if such engulfment did not occur , pathogens released prior to mature infection often are not primed for the second round infection and can be recognized and destroyed more easily by the immune system .
therefore , it is not unexpected to learn that hijacking host cell death pathways constitutes an important pathogenic strategy for almost all well adapted intracellular pathogens . as detailed in the several excellent articles of this review series ,
legionella pneumophila is a facultative intracellular pathogen that uses similar strategies to replicate in phylogenetically distant eukaryotic cells , ranging from amebae to human alveolar macrophages . within these evolutionarily distant host cells ,
the l. pneumophila - containing vacuole ( lcv ) undertakes a unique maturation pathway characterized by the evasion of endocytic fusion and the interception of membrane trafficking vesicles originating from the endoplasmic reticulum ( er ; isberg et al . , 2009 ; hubber and roy , 2010 ) . as the bacterium begins to multiply , active acquisition of membrane materials from the er - derived vesicles compensates the expansion of the lcv .
the result of such remodeling is the formation of a compartment morphologically and cell biologically resembling the er ( isberg et al . , 2009 ) . whereas the interaction with the host membrane trafficking pathways is probably the best understood process during intracellular l. pneumophila growth , it is becoming clear that modulation of several other pathways , including lipid metabolism , autophagy , ubiquitination , and host cell death , is also critical for successful infection ( hubber and roy , 2010 ) . to accomplish this feat , l. pneumophila delivers more than 200 bacterial proteins via the dot / icm type iv secretion system into host cells where they engage in distinct host pathways to facilitate the biogenesis of the lcv permissive for bacterial replication ( ensminger and isberg , 2009 ) . here , i will discuss recent progress in the interplays between l. pneumophila and the host cell death pathways and how such interplays contribute to successful bacterial infection in mammalian cells .
in mammalian cells , programmed cell death is divided into at least four categories : apoptosis , pyroptosis , necrosis , and necroptosis ( fink and cookson , 2005 ; vandenabeele et al . , 2010 ) .
apoptosis is the best characterized programmed cell death mode ; it plays critical roles in development , maintaining tissue homeostasis , shaping the immune repertoire , and restricting the progress of infections ( danial and korsmeyer , 2004 ) .
this cell death mode can be initiated by two distinct but partially overlapping pathways : the extrinsic , receptor - mediated pathway and the intrinsic mitochondrial pathway ( salvesen and riedl , 2008 ) . in both cases , biochemical cascades triggered by extracellular ligands or intracellular damage led to the activation of caspases , which are a family of cysteine - dependent aspartate - specific proteases .
these enzymes mediate most of the apoptotic program and some of them can be blocked by inhibitor of apoptotic proteins ( iaps ; scott et al . , 2005 ) .
the mitochondrion is the central controlling site for the intrinsic apoptotic pathway because it harbors cytochrome c and second mitochondrion - derived activator of caspase ( smac or diablo ) .
the release of cytochrome c into the cytosol leads to the assembly of a supramolecular complex known as the apoptosome , which initiates the caspase activation cascade ( riedl and salvesen , 2007 ) . in parallel , smac neutralizes the caspase - inhibitory activity of xiap , thereby indirectly contributing to the maximal activation of the caspase cascade ( riedl and salvesen , 2007 ) .
the release of these two apoptosis - initiating molecules is caused by perturbation of the integrity of the outer mitochondrial membrane ( omm ) , which is delicately regulated by members of the bcl-2 protein family . based on their roles in controlling apoptosis ,
bcl-2 family proteins can be divided into two subsets : pro- and anti - apoptotic molecules .
members of this protein family can form homo- as well as heterodimers ( chipuk et al .
, 2010 ) . indeed , the formation of heterodimers between pro- and anti - apoptotic members , a process that alters the cellular ratios between these two subsets of proteins , determines at least in part the susceptibility of cells to a death signal ( cory and adams , 2002 ) .
first , they possess up to four conserved bcl-2 homology ( bh ) domains , designated bh1 , bh2 , bh3 , and bh4 ( chipuk et al . , 2010 ) ; however , a number of pro - apoptotic bcl-2 family proteins , such as bid , bim , bad , and bnip3 contain only the bh3 domain and are classified as the 
second , most members of this protein family contain a carboxy - terminal hydrophobic domain , which in many cases is critical for their biological activities by membrane insertion and membrane remodeling ( lomonosova and chinnadurai , 2008 ) .
upon sensing cell stress caused by various insults such as dna damage , cytokine deprivation , or infection , these bh3-only proteins trigger the insertion of the two pro - apoptotic , pore - forming proteins bax and/or bak , into the omm ( chinnadurai et al . , 2008 ) , causing the release of the cytochrome c and smac .
members of the pro - survival proteins , including bcl-2 , bcl - xl , and mcl1 , inhibit apoptosis by directly sequestering bak , bax , and bh3-only proteins to prevent permeabilization of omm ( chipuk et al . , 2010 ) .
apoptosis plays an important role in the defense against pathogens on the level of both the reaction of an individual host cell to an invading microorganism and the reacting immune system ( creagh et al . ,
, successful pathogens have evolved different but often equally effective mechanisms to manipulate host cell death pathways to benefit their proliferation .
such manipulation can be achieved by targeting the activity of one or more host proteins critical in each step of the apoptotic pathways .
for example , many viruses code for proteins that specifically inhibit apoptosis of infected cells by directly interacting with pro - apoptotic members of the bh3-only proteins ( roulston et al .
similarly , some obligate intracellular bacterial pathogens such as chlamydia trachomatis and rickettsia rickettsii actively inhibit apoptosis ( clifton et al . , 1998 ; fan et al . , 1998 )
of particular interest is that c. trachomatis prevents infected cells from undergoing apoptosis by specifically degrading members of the pro - death bh3-only proteins ( dong et al . , 2005 ) , probably by the chlamydia protease - like factor ( cpaf ;
accumulating evidence indicates that l. pneumophila is able to manipulate host cell death pathways by targeting regulatory molecules with diverse mechanisms at different points of the signaling cascade .
earlier studies suggest that l. pneumophila actively induces apoptosis of infected cells via the activation of the executioner caspase , caspase-3 ( gao and abu kwaik , 1999a , b ; molmeret et al . , 2004 ) .
in permissive cell lines such as u937 or human peripheral blood monocytes , infection by l. pneumophila caused high level apoptosis within the first 3  h of bacterial uptake , and , in some cases , apoptotic cells reached 100% ( gao and abu kwaik , 1999b ) .
however , a later study found that despite vigorous bacterial replication over a time span of 13  h , the proportion of apoptotic cells did not significantly increase in permissive macrophages , suggesting that in permissive host cells , l. pneumophila actively inhibits infected cells from undergoing apoptosis ( derre and isberg , 2004 ) .
this notion was validated by the discovery of sdha and sidf , two dot / icm substrates involved in the inhibition of host cell death .
mouse bone marrow macrophages infected with a sdha mutant became apoptotic , displaying increased nuclear degradation , mitochondrial disruption , membrane permeability , and caspase activation , indicating a role for sdha in inhibiting host cell death ( laguna et al . , 2006 ) .
interestingly , the requirement for sdha is cell - type specific because the growth defect was less severe in the ameba dictyostelium discoideum or the more permissive u937 cell - derived macrophages ( laguna et al . , 2006 ) .
sdha appears to be multifunctional because it also plays a key role in the suppression of type i interferons ( ifn ) response in macrophages infected by l. pneumophila ( monroe et al . , 2009 ) .
although less severe , cells infected by mutants lacking sidf also exhibited higher levels of apoptosis , which led to marginal but detectable defects in intracellular growth ( banga et al .
sidf interacts and inhibits the cell death - inducing activity of bnip3 and bcl - rambo , two non - canonical pro - apoptotic members of bcl-2 protein family ( banga et al .
, 2007 ; figure 1 ) . both mitochondrial proteins , bnip3 and bcl - rambo appears to induce apoptosis by different mechanisms .
bnip3 appear to play a role in cellular response to stress ( chinnadurai et al . , 2008 ) .
on the other hand , bcl - rambo induces cell apoptosis specifically blocked by the caspase inhibitors , iaps ( kataoka et al .
thus , it is attempting to speculate that sidf plays a role in making infected cells less sensitive to stress caused by l. pneumophila infection .
nevertheless , the activities of sdha and sidf indicate that l. pneumophila inhibits host apoptosis by directly targeting host proteins involved in controlling the cell death pathways at the mitochondrion ( figure 1 ) .
internalized l. pneumophila translocates a large number of effectors into host cytosol via the dot / icm type iv secretion system . a yet unidentified set of effectors trigger an imbalance between the pro - death and pro - survival members of the bcl-2 protein family , leading to the insertion of bax / bak into the mitochondrial out membrane , thus the release of cytochrome c and subsequent activation of the caspases 3 and 7 .
another set of effectors , including legk1 and lnab , activate nf- b , most likely by initiating the kinase cascade that ultimately causes phosphorylation and subsequent degradation of i b , the inhibitor of nf- b , leading to nucleus translocation of nf- b and the induction of anti - apoptotic genes such as xiap and pai-2 , whose product inhibits cell death by targeting caspases 3 and 7 .
activation of the map kinase pathway by another set of unknown effectors can lead to similar effects .
a third set of proteins such as sidf and sdha , which inhibit host cell death by targeting pro - apoptotic proteins bnip3 and bcl - rambo or by unknown mechanisms . in non - permissive mouse macrophages ,
flagellin reached the cytosol probably via the dot / icm transporter is sensed by the nlr receptor naip5 , which together with ipaf and the inflammasome activates caspase-1 , leading to pyroptotic cell death .
creb , camp response element - binding protein ; lcv , legionella containing vacuole ; mapk , mitogen - activated protein kinases ; mito , mitochondrion ; n , nucleus ; naip5 , nlr family , apoptosis inhibitory protein 5 ; pai-2 , plasminogen activator inhibitor-2 ; xiap , x - linked inhibitor of apoptosis protein .
another layer of cell death inhibition mechanisms utilized by l. pneumophila is revealed by experiments designed to examine host gene expression profiles in response to low dose bacterial challenge ( abu - zant et al .
several groups of genes known to be directly or indirectly involved in regulating host cell death were significantly induced , including stress response genes such as heat shock protein genes s and pro - survival members of the bcl-2 protein family ( losick and isberg , 2006 ; abu - zant et al .
interestingly , the most striking induction was observed in a collection of anti - apoptotic genes positively regulated at a transcriptional level by the regulator nuclear factor-b ( nf-b ; losick and isberg , 2006 ; abu - zant et al .
, 2007 ; figure 1 ) . consistently , in human and permissive mouse macrophages ,
infection by wild type l. pneumophila led to nuclear translocation of nf-b ( losick and isberg , 2006 ; abu - zant et al .
thus , l. pneumophila is able to inhibit mammalian cell death by increasing the levels of anti - apoptotic proteins at transcriptional level .
two lines of evidence indicate that the induction of these anti - apoptotic genes is important for productive l. pneumophila infection .
first , bacterial challenge of macrophages lacking one such anti - apoptotic gene , the plasminogen activator inhibitor-2 ( pai-2 ) , led to significantly higher levels of apoptosis and reduction in bacterial replication ( losick and isberg , 2006 ) .
second , inhibition of nuclear translocation of nf-b by genetic or pharmaceutical agents caused extensive cell death upon low dose bacterial challenge ( losick and isberg , 2006 ) .
proteins like bcl-2 can interact and inhibit the activity of several pro - death bh3-only proteins ( chipuk et al . , 2010 ) .
on the other hand , iaps such as xiap can directly neutralize the activity of caspase-3 and -7 ( scott et al .
interestingly , the observed nf-b activation is involved in a signaling pathway independent of the toll - like receptor ( tlr ) adaptor myd88 and the cytoplasmic sensor nod1 , but is absolutely dependent upon the dot / icm secretion system ( losick and isberg , 2006 ) .
consistent with this notion , legk1 and lnab , two dot / icm substrates with such activity have been identified in screenings using nf-b responsive reporters and constructs that direct the expression of individual bacterial genes ( ge et al . , 2009 ; losick et al . , 2010 ;
whereas the biochemical mechanisms of lnab is unknown , legk1 , appears to directly target ib and other ib family of inhibitors including p100 in the non - canonical nf-b pathway by phosphorylation ( ge et al . , 2009 ) .
similar to most characterized dot / icm substrates , deletion of legk1 or lnab resulted in little or only partial reduction in nf-b activation .
consistently , such mutants did not exhibit defects in intracellular bacterial growth ( ge et al .
first , proteins such as sdha and sidf that target host cell death proteins can provide the protection in these mutants .
second , other yet unidentified effectors may contribute to the activation of nf-b during the infection by these mutants .
this is very likely because functional redundancy has been observed in effectors targeting other cellular pathways important for l. pneumophila intracellular growth ( isberg et al . , 2009 ) .
third , as documented in a recent report , nf-b activated by a non - canonical mechanism may also contribute to the induction of anti - apoptotic genes . in the study , fontana et al .
( 2011 ) found that in conjunction with classic pathogen associated molecular pattern ( pamp ) molecules , inhibition of host protein synthesis by several dot / icm substrates led to prolonged activation of nf-b , thus strong induction of a set of inflammatory cytokines such as interleukin ( il)-23 and granulocyte  macrophage colony stimulating factor .
such activation was achieved by the failure to resynthesize ib , the short - lived inhibitor of the nf-b transcription factor in the presence of these protein synthesis inhibitors ( fontana et al . , 2011 ) .
finally , some substrates of the dot / icm transporter also potently activate the map kinase pathway , which could also lead to induction of the anti - apoptotic genes such as pai-2 ( shin et al .
, 2008 ) . given the high level of conservation in the mapk pathway in eukaryotes , it is not unexpected that the activation of this pathway also occurs in the ameba d. discoideum ( li et al . , 2009 ) . clearly ,
inhibition of host cell apoptosis by l. pneumophila is achieved by collective activities of various bacterial proteins ; these proteins either directly or indirectly , reprogram the various cell death pathways to ensure maximal bacterial replication .
in contrast to the many lines of evidence directly supporting active inhibition of host cell death by l. pneumophila , evidence pointing to the induction of apoptosis by this bacterium mostly is indirect .
the apoptotic phenotypes associated with host cells infected with mutants lacking one or more cell death inhibiting dot / icm substrates such as sdha and sidf suggested the existence of effectors capable of inducing cell death ( laguna et al . , 2006 ; banga et al . , 2007 ) .
apoptosis induced by l. pneumophila is extremely apparent in specialized phagocytes such as dendritic cells . in these cells ,
infection by l. pneumophila induced a caspase-3-dependent apoptotic pathway that aborted intracellular bacterial replication in the early phase of infection ( nogueira et al . , 2009 ; figure 1 ) .
interestingly , dendritic cells from mice deficient in bak and bax ( bak bax ) or mice overexpressing the pro - survival protein bcl-2 are able to support intracellular bacterial infection without undergoing apoptosis ( nogueira et al . , 2009 ) , indicating that infection by l. pneumophila activates the mitochondrial pathway of apoptosis by inducing an imbalance between the pro - apoptotic and pro - survival members of bcl-2 protein family .
the activation of the mitochondrial apoptotic pathway also occurs in macrophages but at a much slower pace ( abu - zant et al .
the drastic differences between macrophages and dendritic cells in response to cell death stimuli such as those caused by l. pneumophila infection may be due to higher level or more active of the putative receptors or sensor proteins responsible for engaging the cell death signals from the bacterium in the latter cell type .
it has been proposed that the sensitivity to pathogens exhibited by dendritic cells serves a protective role to the host by preventing infectious agents from using these cells as vehicles to reach deep tissues of the organism ( nogueira et al . , 2009 ) .
for example , shiga toxins trigger apoptosis in many cell types , probably by inducing stress in the er through inhibition of protein synthesis ( lee et al . , 2008 ) .
although a number of dot / icm substrates toxic to both yeast and mammalian cells have been identified , none of them has been shown to specifically induce host cell death .
for example , at least five l. pneumophila proteins capable of inhibiting host protein synthesis have been reported .
when overexpressed , these proteins are highly toxic to host cells ( belyi et al . , 2006 , 2008 ; shen et al .
, 2009 ; fontana et al . , 2011 ) . however , whether these proteins play roles in the induction of host cell death under infection conditions is unknown .
it is worth noting that a mutant lacking all five genes failed to induce prolonged nf-b activation , suggesting that these toxic proteins contribute to protect host cell death during infection ( fontana et al . , 2011 ) .
such outcomes clearly are opposite to the phenotypes observed by overexpressing these proteins in host cells . nevertheless , since the dot / icm transporter but not bacterial replication is required for the activation of the apoptotic pathway ( abu - zant et al . , 2005 ;
either the activities of one or more dot / icm substrates or the  stress  caused by the collective impact of multiple effectors , or a combination of both can be the mechanism used by the bacterium for such activation .
interestingly , macrophages lacking bak and bax or overexpressing bcl-2 still undergo extensive apoptosis upon being challenged by the sdha mutant ( nogueira et al . , 2009 ) , suggesting that l. pneumophila is able to activate a cell death pathway independent of several critical components of the mitochondrial apoptotic pathway .
besides the mitochondrial apoptotic pathway , l. pneumophila also induces pyroptosis , a form of inflammatory cell death in macrophages from mice harboring a functional naip5 allele ( fortier et al . , 2005 ) .
naip5 is a member of the nod - like receptor ( nlr ) family , and an important component of the cytosolic protein complexes called inflammasomes , which induce autoactivation of caspase-1 ( figure 1 ) .
the nlrs are considered functionally equivalent to the tlrs localized on the surface or within endosomes of immune cell ( davis et al . , 2011 ) , which recognize components of the pathogen called pamps such as bacterial flagellin , peptidoglycan , and nucleic acid variants ( west et al . , 2006 ) .
caspase-1 induced pyroptosis , which is accompanied by the release of mature il-1 and il-18 and other cytokines , and is inherently proinflammatory ( fink and cookson , 2006 ) .
the observation that flagellin deficient l. pneumophila mutants gained the ability to grow productively in macrophages expressing functional naip5 without activating caspase-1-dependent cell death indicates that flagellin activates naip5 in the cytoplasm ( molofsky et al . , 2006 ; ren et al . , 2006 ) . a subsequent study using a retroviral transduction to express flagellin directly in macrophages clearly showed that flagellin is necessary and sufficient for the pyroptosis induction via a pathway controlled by the ipaf inflammasome ( lightfield et al . , 2008 ) .
consistently , macrophages from naip5-deficient mice completely failed to activate caspase-1 and were able to support robust growth of wild type l. pneumophila , further indicating that flagellin - mediated pyroptosis induction absolutely requires a functional naip5 ( lightfield et al . , 2008 ) .
thus , the induction of pyroptosis in immune cells from restrictive mice by l. pneumophila flagellin is  accidental ,  but has provided an excellent model to dissect the host immune surveillance mechanisms ( vance , 2010 ) .
clearly , in permissive mammalian cells , infection by l. pneumophila activates the classic mitochondrial apoptotic pathway and possibly other yet unrecognized cell death processes , leading to activation of several caspases , including caspases 3 and 7 .
concurrently , the bacterium employs a combination of mechanisms to inhibit infected cells from fully executing the apoptotic cascade to allow productive bacterial replication . whereas the benefit of inhibiting apoptosis by l. pneumophila is obvious , the benefit for activation this pathway is less clear .
activated caspases may participate in cellular processes in not directly related to cell death but important for the biogenesis of the lcv .
for example , active caspase-3 appears to cleave rabaptin-5 , an rab5 effector , and cell treated with caspase-3 inhibitor ( devd - fmk ) or the pan inhibitor of caspases ( z - vad - fmk ) abolished intracellular bacterial growth ( molmeret et al . , 2004 ) .
however , in contrast to this observation , macrophages from permissive mice ( a / j ) lacking caspase-3 ( casp3 ) still support robust intracellular bacterial growth ( zamboni et al .
these discrepancies may result from the non - specificity of the caspase inhibitors or the different host cells used in these studies .
2010 ) showed that cleavage of bacterial effectors by caspase-3 is important for pathogenicity of salmonella enterica serovar typhimurium .
it is possible that active caspases also participate in cellular processes that are not directly related to cell death but are important in the biogenesis of the lcv .
a number of important questions remain outstanding in our understanding of the modulation of host cell death pathways by l. pneumophila .
first , it is believed that amebae but not metazoan play a role in the evolution of l. pneumophila pathogenicity . however , nf-b signaling and many components of apoptosis have now been documented to be targeted by proteins of this bacterium are not present in amebae cells .
it is possible that l. pneumophila co - evolved with some as yet unrecognized lower metazoan . in support of this notion
, two recent studies found that l. pneumophila can infect caenorhabditis elegans ( brassinga et al .
, 2010 ; komura et al . , 2010 ) , an organism with well - defined cell death pathways ( conradt , 2009 ) .
alternatively , l. pneumophila can acquire genes specific for metazoan pathways from other more adapted mammalian pathogens which also are parasites of amebae ( moliner et al . , 2010 ) .
second , the nature of the bacterial signals that triggers the mitochondrial cell death pathway remains elusive .
particularly , the sdha mutant appears to elicit a cell death pathway independent of the canonical mitochondrial pathway controlled by the bcl-2 protein family ( nogueira et al . , 2009 ) .
research in this field aiming at characterizing both pro - survival and pro - death l. pneumophila proteins and their host targets will surely generate more exciting discoveries in years to come .
the author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest .
 programmed cell death is considered the ultimate solution for the host to eliminate infected cells , leading to the abolishment of the niche for microbial replication and the ablation of infection . 
 thus , it is not surprising that successful pathogens have evolved diverse strategies to reprogram the cell death pathways for their proliferation . 
 using effector proteins translocated by the dot / icm type iv secretion system , the facultative intracellular pathogen legionella pneumophila manipulates multiple host cellular processes to create a niche within host cells to support its replication . 
 investigation in the past decade has established that in mammalian cells this bacterium actively modulates two host cell death pathways , namely the canonical apoptotic pathway controlled by the mitochondrion and the pyroptotic pathway controlled by the nod - like receptor naip5 and the ipaf inflammasome . in this review 
 , i will discuss the recent progress in understanding the mechanisms the bacterium employs to interfere with these host cell death pathways and how such modulation contribute to the intracellular life cycle of the pathogen . 
Introduction
Host Cell Death Pathways and their Regulation
Regulation of Host Cell Death Pathways by 
Potential Benefits of 
Concluding Remarks and Prospectives
Conflict of Interest Statement