wild - type , pigmented long - evans ( le ) rats , obtained from charles river ( seattle , wa , usa ) , were used as controls because although the retina layers thin with age , there is no appreciable cell loss or change in total retinal volume .
in addition , le rats have previously been used to back breed pigment in the rcs line and to generate the nondystrophic rcs line .
rats were housed in standard conditions under a 12/12-hour light  dark cycle ( light cycle : 200 lux ) .
all experiments were approved by the institutional animal care and use committee at ohsu and adhered to the arvo statement for the use of animals in ophthalmic and vision research .
rcs rats were imaged weekly from p17 to p60 and final images were obtained at p90 .
both right ( od ) and left ( os ) eyes were imaged from 7 to 10 different rcs rats at each time point .
both od and os were imaged from four different le rats at each time point . before imaging , pupils were dilated with 1% tropicamide and 2.5% phenylephrine .
sedation was induced by ketamine ( 80 mg / kg)/xylazine ( 5 mg / kg ) .
spectral - domain oct images were obtained by using the envisu r2200-hr sd - oct device ( bioptigen , durham , nc , usa ) with the reference arm placed at approximately 1187 mm .
single horizontal and vertical linear scans ( 2-mm preset scan width , 1500 a - scans / b - scan  20 frames / b - scan ) were obtained first while centered on the optic nerve , then with the nerve displaced either temporally / nasally or superiorly / inferiorly . to analyze interscan variability , three consecutive od and os temporal ,
nasal , superior , and inferior scans were obtained in three additional rcs rats at p30 .
axial and transverse export resolution was 1.175 m / pixel and 3.175 m / pixel , respectively .
these files were loaded into imagej ( version 1.45 ; provided in the public domain by the national institutes of health , bethesda , md , usa ) where they then were registered by using the stackreg plug - in and averaged as a z - stack .
manual segmentation and quantification of retinal layer thickness was performed with a custom - designed sd - oct segmentation program built in igor pro ( igor pro 6.12 ; wavemetrics , inc . , lake oswego , or , usa ) .
segmentation software is sharable with permission of the author ( yuquan wen ) . for segmentation analysis , total retina ( tr ) , inl , outer nuclear layer+
the onl+ , the photoreceptor layer from the outer limiting membrane ( olm ) to the inl / outer plexiform layer ( opl ) interface , was used to enable consistent measurements across ages as hyperreflectivity in the photoreceptors increased . from p30 to p90 , a hyperreflective layer ( hrl ) was observed and measured in rcs rats . to ensure our segmentation analysis was reliable and repeatable , interscan and interanimal variability
were assessed with intraclass correlation coefficients ( icc ) and 95% confidence intervals by using ibm ( armonk , ny , usa ) spss statistics version 24 ( tables 1 , 2 ) .
to calculate interscan variability , three repeated scan measurements from three different p30 rcs rats were correlated ( table 1 ) . to calculate
the variation of od and os scans , measurements from nine different p30 rcs rats were correlated ( table 2 ) .
reproducibility of first , second , and third scan retinal layer thickness measurements retinal layer thickness variation between rcs od and os scans at p30 total retina , inl , onl+ , rpe , and hrl thicknesses ( micrometers , y - axis ) were measured 40 outward in the inferior ( od and os ) , nasal ( os ) , or temporal ( od ) direction and + 40 outward in the superior ( od and os ) , nasal ( od ) , or temporal ( os ) direction from the optic nerve head ( x - axis ) . the resulting waves for tr and onl+ in the superior and inferior retina were averaged and plotted as spider graphs .
additionally , points from 40 to 25 and 25 to 40 were averaged to obtain tr , inl , onl+ , rpe , and hrl thicknesses for each eye in each quadrant .
right and left eye thicknesses were averaged and plotted against age ( days ) for all of the animals scanned .
, la jolla , ca , usa ) prism 7 software and plotted for each data set .
a 2-way anova , multiple comparisons test was used to compare average rcs retinal layer thickness between each quadrant at each time point .
once enucleated , eyes were placed immediately in prefer , a glyoxal fixative ( anatech ltd , battle creek , mi , usa ) and incubated overnight at room temperature .
orientated eyes were processed and embedded in paraffin for sectioning ( tissue - tek vip 6 , tissue - tek tec 5 ; sakura finetek usa , inc . ,
sections were cut with a microtome to a thickness of 4 m , stained with hematoxylin - eosin ( h&e ) , and viewed on a leica dmi3000 b microscope ( leica microsystems gmbh , wetzlar , germany ) .
wild - type , pigmented long - evans ( le ) rats , obtained from charles river ( seattle , wa , usa ) , were used as controls because although the retina layers thin with age , there is no appreciable cell loss or change in total retinal volume .
in addition , le rats have previously been used to back breed pigment in the rcs line and to generate the nondystrophic rcs line .
rats were housed in standard conditions under a 12/12-hour light  dark cycle ( light cycle : 200 lux ) .
all experiments were approved by the institutional animal care and use committee at ohsu and adhered to the arvo statement for the use of animals in ophthalmic and vision research .
rcs rats were imaged weekly from p17 to p60 and final images were obtained at p90 .
both right ( od ) and left ( os ) eyes were imaged from 7 to 10 different rcs rats at each time point .
both od and os were imaged from four different le rats at each time point . before imaging , pupils were dilated with 1% tropicamide and 2.5% phenylephrine .
sedation was induced by ketamine ( 80 mg / kg)/xylazine ( 5 mg / kg ) .
spectral - domain oct images were obtained by using the envisu r2200-hr sd - oct device ( bioptigen , durham , nc , usa ) with the reference arm placed at approximately 1187 mm .
single horizontal and vertical linear scans ( 2-mm preset scan width , 1500 a - scans / b - scan  20 frames / b - scan ) were obtained first while centered on the optic nerve , then with the nerve displaced either temporally / nasally or superiorly / inferiorly . to analyze interscan variability , three consecutive od and os temporal ,
nasal , superior , and inferior scans were obtained in three additional rcs rats at p30 .
axial and transverse export resolution was 1.175 m / pixel and 3.175 m / pixel , respectively .
these files were loaded into imagej ( version 1.45 ; provided in the public domain by the national institutes of health , bethesda , md , usa ) where they then were registered by using the stackreg plug - in and averaged as a z - stack .
manual segmentation and quantification of retinal layer thickness was performed with a custom - designed sd - oct segmentation program built in igor pro ( igor pro 6.12 ; wavemetrics , inc . , lake oswego , or , usa ) .
segmentation software is sharable with permission of the author ( yuquan wen ) . for segmentation analysis , total retina ( tr ) , inl ,
the onl+ , the photoreceptor layer from the outer limiting membrane ( olm ) to the inl / outer plexiform layer ( opl ) interface , was used to enable consistent measurements across ages as hyperreflectivity in the photoreceptors increased .
from p30 to p90 , a hyperreflective layer ( hrl ) was observed and measured in rcs rats . to ensure our segmentation analysis was reliable and repeatable , interscan and interanimal variability
were assessed with intraclass correlation coefficients ( icc ) and 95% confidence intervals by using ibm ( armonk , ny , usa ) spss statistics version 24 ( tables 1 , 2 ) .
to calculate interscan variability , three repeated scan measurements from three different p30 rcs rats were correlated ( table 1 ) . to calculate
the variation of od and os scans , measurements from nine different p30 rcs rats were correlated ( table 2 ) .
reproducibility of first , second , and third scan retinal layer thickness measurements retinal layer thickness variation between rcs od and os scans at p30 total retina , inl , onl+ , rpe , and hrl thicknesses ( micrometers , y - axis ) were measured 40 outward in the inferior ( od and os ) , nasal ( os ) , or temporal ( od ) direction and + 40 outward in the superior ( od and os ) , nasal ( od ) , or temporal ( os ) direction from the optic nerve head ( x - axis ) . the resulting waves for tr and onl+ in the superior and inferior retina were averaged and plotted as spider graphs .
additionally , points from 40 to 25 and 25 to 40 were averaged to obtain tr , inl , onl+ , rpe , and hrl thicknesses for each eye in each quadrant .
right and left eye thicknesses were averaged and plotted against age ( days ) for all of the animals scanned .
best - fit equations were generated by using graphpad ( graphpad software , inc . , la jolla , ca , usa ) prism 7 software and plotted for each data set .
a 2-way anova , multiple comparisons test was used to compare average rcs retinal layer thickness between each quadrant at each time point .
once enucleated , eyes were placed immediately in prefer , a glyoxal fixative ( anatech ltd , battle creek , mi , usa ) and incubated overnight at room temperature . eyes were then placed in cassettes and stored in 70% ethanol at room temperature .
orientated eyes were processed and embedded in paraffin for sectioning ( tissue - tek vip 6 , tissue - tek tec 5 ; sakura finetek usa , inc . , torrance , ca , usa ) .
sections were cut with a microtome to a thickness of 4 m , stained with hematoxylin - eosin ( h&e ) , and viewed on a leica dmi3000 b microscope ( leica microsystems gmbh , wetzlar , germany ) .
retinal layers including the ganglion cell layer ( gcl ) , inner plexiform layer ( ipl ) , inl , opl , onl , olm , photoreceptor inner and outer segments ( pr ) , and rpe were clearly visible in sd - oct images of le rats at all ages examined ( fig .
the ellipsoid zone ( ez ) , which consists of mitochondria within the ellipsoid layer of the outer portion of the inner segments and represents the inner / outer segment junction , could be identified from p17 to p23 , but the outer segments ( os ) were becoming hyperreflective , which caused them to become isoreflective with the ez .
thus , differentiation between the ez and os was less distinct in p17 to p23 rcs retinas than in le controls . by p30 , the pr and olm were indistinguishable and isoreflective .
thus , from p30 to p90 , this area of the retina was observed as a single layer and termed  the hyperreflective layer  ( hrl ) . from p30 to p60 the onl became progressively hyperreflective and thinned relative to baseline . by p45 , it became isoreflective with the opl and became difficult to distinguish , but was not detectable by p90 ( fig .
images acquired with sd - oct illustrate the longitudinal retinal degeneration in the rcs rat .
representative linear sd - oct scans from right eyes of long - evans and rcs rats at differing ages from p17 to p90 .
images are representative of the same location in both long - evans and rcs retinas , approximately 30 from the optic nerve head in each retinal quadrant . each image is approximately 2. photoreceptors were maintained in long - evans retinas and degenerated over time in rcs retinas .
is , inner segments ; onl , outer nuclear layer ; os , outer segments . to characterize the progressive retinal degeneration in the rcs rat
, manual segmentation was performed to calculate tr , inl , onl+ , hrl , rpe thickness ( see schematic for segmentation lines in figure legend ; fig .
to validate our segmentation analysis , interscan and interanimal variability were assessed with icc ( tables 1 , 2 ) .
our manual segmentation provided reproducible retinal layer thickness measurements , as most thickness measurements , obtained from three individual scans , were correlative ( icc = 0.51 ; table 1 ) .
there was also good correlation ( icc = 0.6440.944 ) between retinal layer thickness measurements obtain from right and left eyes of p30 rcs rats , suggesting that degeneration rates were consistent between eyes ( table 2 ) .
( a ) representative linear sd - oct scans of superior retina from right eyes of long - evans and rcs rats .
schematic for segmentation lines : tr , bottom purple line to the top red line ; inl , blue line to the green line that surround the inl label ; outer nuclear layer plus outer plexiform layer ( onl+ ) , red line to blue line that surround the onl+ label ; rpe , purple line to the blue line ( le p30p90 and rcs p17p23 ) or purple line to the yellow line ( rcs p30p90 ) that surround rpe label ; hrl , yellow line to the red line ( rcs p30p60 ) or yellow line to the blue line ( rcs p90 ) that surround hrl label .
( b ) right eye and left eye tr , onl+ , inl , rpe , and hrl thicknesses from long - evans and rcs retinas in each retinal quadrant were averaged and plotted against age .
rcs tr and onl+ degeneration was best fit with an exponential decay function , whereas all other data points were best fit with linear regressions .
a 2-way anova , multiple comparisons test was used to compare average rcs retinal layer thickness between each quadrant at each time point . *
thickness of the superior retina was significantly increased compared to all other quadrants at specified age ( p  0.01 ) .
* * thickness of the superior retina was significantly increased compared to temporal and inferior retina at specified age ( p  0.01 ) .
long - evans : n = 4 animals ; rcs : n = 710 animals . retinal layers and
segmentation remained consistent from p30 to p90 in le rats . in rcs rats , segmentation changed at p30 due to the appearance of the hrl and at p90 due to the loss of the onl+ ( fig .
scatter plots of average tr and onl+ thickness obtained from rcs rats over time demonstrated considerable declines that were best fit , based on r values , with exponential decay functions ( fig .
in contrast , all other retinal layers quantified in both rcs rats and le controls were best fit with linear functions ( fig . 2b ; supplementary table s1 ) .
these data demonstrated that total retinal degeneration in the rcs rat was primarily due to the loss of photoreceptors , which is highlighted in figure 3 .
spider graphs of the superior and inferior retina illustrate that average tr and onl+ thickness decreased consistently over time in the rcs rat , whereas le rats had a narrow range in average tr and onl+ thickness from p30 to p90 ( fig .
scatter plots in figure 2 also demonstrated the consistency of retinal layer thickness between the different retinal quadrants .
superior hrl thickness was significantly increased as compared to nasal , temporal , and inferior hrl thickness from p30 to p56 ( fig . 2b , supplementary table s2 ; p  0.01 ) .
at p60 , superior hrl thickness was still significantly increased as compared to temporal and inferior hrl thickness . by p90 , hrl thickness was not significantly different in any retinal quadrant ( fig .
not surprisingly , average superior tr thickness was affected and was also significantly increased as compared to nasal , temporal , and inferior tr thickness from p30 to p37 ( fig . 2b , supplementary table s2 ; p  0.01 ) .
interestingly , superior onl+ was thicker than all other retinal quadrants at p37 ( fig . 2b , supplementary table s2 ; p  0.01 ) .
rcs total retina and onl+ thicknesses obtained from right eye and left eye inferior and superior sd - oct scans were averaged and plotted versus retinal eccentricity from the optic nerve head .
gray shading represents long - evans total retina and onl+ range from p30 to p90 .
rcs onl+ is undetectable by sd - oct at p90 and thus is plotted as zero ( * ) .
histology of retinal sections from p21 , p30 , p45 , and p60 rcs rats was compared to locally matched sd - oct images ( fig .
4 ) . at p21 the gcl , inl , opl , onl , pr , and rpe were clearly observable in both histology and sd - oct images . at this age , os disorganization was only evident at the apical side of the rpe .
by p30 , os disorganization expanded and the correlation between the pr / debris layer and the hrl was evident , suggesting that both layers are comprised of is , disorganized os , and debris ( fig .
, both modalities demonstrated that this layer is thicker in the superior retina and thins over time ( fig .
the onl has significantly degenerated and less than half of the onl remained , which was clearly visible in the histology images . in the sd - oct images ,
the onl became hyperreflective , isoreflective with the opl , and was more difficult to distinguish than in the retinal sections ( fig .
although the onl resolution declined with disease progression , an onl+ layer was still measurable in sd - oct images at this time point ( fig .
when comparing the histology and sd - oct images from p21 to p60 , both demonstrated an observable decrease in tr and onl thickness ( fig .
representative histology and sd - oct images of superior and inferior quadrants of the posterior retina from right eyes of rcs rats at p21 ( a ) , p30 ( b ) , p45 ( c ) , and p60 ( d ) .
the hrl correlates with the pr / debris layer indicating a composition of is , disorganized os , and debris ( arrowheads ) .
retinal layers including the ganglion cell layer ( gcl ) , inner plexiform layer ( ipl ) , inl , opl , onl , olm , photoreceptor inner and outer segments ( pr ) , and rpe were clearly visible in sd - oct images of le rats at all ages examined ( fig .
the ellipsoid zone ( ez ) , which consists of mitochondria within the ellipsoid layer of the outer portion of the inner segments and represents the inner / outer segment junction , could be identified from p17 to p23 , but the outer segments ( os ) were becoming hyperreflective , which caused them to become isoreflective with the ez .
thus , differentiation between the ez and os was less distinct in p17 to p23 rcs retinas than in le controls . by p30 , the pr and olm were indistinguishable and isoreflective .
thus , from p30 to p90 , this area of the retina was observed as a single layer and termed  the hyperreflective layer  ( hrl ) . from p30 to p60 the onl became progressively hyperreflective and thinned relative to baseline . by p45 , it became isoreflective with the opl and became difficult to distinguish , but was not detectable by p90 ( fig .
images acquired with sd - oct illustrate the longitudinal retinal degeneration in the rcs rat .
representative linear sd - oct scans from right eyes of long - evans and rcs rats at differing ages from p17 to p90 .
images are representative of the same location in both long - evans and rcs retinas , approximately 30 from the optic nerve head in each retinal quadrant . each image is approximately 2. photoreceptors were maintained in long - evans retinas and degenerated over time in rcs retinas .
is , inner segments ; onl , outer nuclear layer ; os , outer segments .
to characterize the progressive retinal degeneration in the rcs rat , manual segmentation was performed to calculate tr , inl , onl+ , hrl , rpe thickness ( see schematic for segmentation lines in figure legend ; fig .
2a ) . to validate our segmentation analysis , interscan and interanimal variability were assessed with icc ( tables 1 , 2 ) .
our manual segmentation provided reproducible retinal layer thickness measurements , as most thickness measurements , obtained from three individual scans , were correlative ( icc = 0.51 ; table 1 ) .
there was also good correlation ( icc = 0.6440.944 ) between retinal layer thickness measurements obtain from right and left eyes of p30 rcs rats , suggesting that degeneration rates were consistent between eyes ( table 2 ) .
( a ) representative linear sd - oct scans of superior retina from right eyes of long - evans and rcs rats .
schematic for segmentation lines : tr , bottom purple line to the top red line ; inl , blue line to the green line that surround the inl label ; outer nuclear layer plus outer plexiform layer ( onl+ ) , red line to blue line that surround the onl+ label ; rpe , purple line to the blue line ( le p30p90 and rcs p17p23 ) or purple line to the yellow line ( rcs p30p90 ) that surround rpe label ; hrl , yellow line to the red line ( rcs p30p60 ) or yellow line to the blue line ( rcs p90 ) that surround hrl label .
( b ) right eye and left eye tr , onl+ , inl , rpe , and hrl thicknesses from long - evans and rcs retinas in each retinal quadrant were averaged and plotted against age .
rcs tr and onl+ degeneration was best fit with an exponential decay function , whereas all other data points were best fit with linear regressions .
a 2-way anova , multiple comparisons test was used to compare average rcs retinal layer thickness between each quadrant at each time point . *
thickness of the superior retina was significantly increased compared to all other quadrants at specified age ( p  0.01 ) . * * thickness of the superior retina was significantly increased compared to temporal and inferior retina at specified age ( p  0.01 ) .
long - evans : n = 4 animals ; rcs : n = 710 animals . retinal layers and
segmentation remained consistent from p30 to p90 in le rats . in rcs rats , segmentation changed at p30 due to the appearance of the hrl and at p90 due to the loss of the onl+ ( fig .
scatter plots of average tr and onl+ thickness obtained from rcs rats over time demonstrated considerable declines that were best fit , based on r values , with exponential decay functions ( fig .
in contrast , all other retinal layers quantified in both rcs rats and le controls were best fit with linear functions ( fig .
these data demonstrated that total retinal degeneration in the rcs rat was primarily due to the loss of photoreceptors , which is highlighted in figure 3 .
spider graphs of the superior and inferior retina illustrate that average tr and onl+ thickness decreased consistently over time in the rcs rat , whereas le rats had a narrow range in average tr and onl+ thickness from p30 to p90 ( fig .
scatter plots in figure 2 also demonstrated the consistency of retinal layer thickness between the different retinal quadrants .
superior hrl thickness was significantly increased as compared to nasal , temporal , and inferior hrl thickness from p30 to p56 ( fig . 2b , supplementary table s2 ; p  0.01 ) .
at p60 , superior hrl thickness was still significantly increased as compared to temporal and inferior hrl thickness . by p90 ,
hrl thickness was not significantly different in any retinal quadrant ( fig . 2b , supplementary table s2 ; p  0.01 ) .
not surprisingly , average superior tr thickness was affected and was also significantly increased as compared to nasal , temporal , and inferior tr thickness from p30 to p37 ( fig . 2b , supplementary table s2 ; p  0.01 ) .
interestingly , superior onl+ was thicker than all other retinal quadrants at p37 ( fig .
rcs total retina and onl+ thicknesses obtained from right eye and left eye inferior and superior sd - oct scans were averaged and plotted versus retinal eccentricity from the optic nerve head .
gray shading represents long - evans total retina and onl+ range from p30 to p90 .
rcs onl+ is undetectable by sd - oct at p90 and thus is plotted as zero ( * ) .
histology of retinal sections from p21 , p30 , p45 , and p60 rcs rats was compared to locally matched sd - oct images ( fig .
4 ) . at p21 the gcl , inl , opl , onl , pr , and rpe were clearly observable in both histology and sd - oct images . at this age , os disorganization was only evident at the apical side of the rpe . by p30 ,
os disorganization expanded and the correlation between the pr / debris layer and the hrl was evident , suggesting that both layers are comprised of is , disorganized os , and debris ( fig .
, both modalities demonstrated that this layer is thicker in the superior retina and thins over time ( fig .
4 ) . by p45 the onl has significantly degenerated and less than half of the onl remained , which was clearly visible in the histology images . in the sd - oct images , the onl became hyperreflective , isoreflective with the opl , and was more difficult to distinguish than in the retinal sections ( fig .
although the onl resolution declined with disease progression , an onl+ layer was still measurable in sd - oct images at this time point ( fig .
when comparing the histology and sd - oct images from p21 to p60 , both demonstrated an observable decrease in tr and onl thickness ( fig .
representative histology and sd - oct images of superior and inferior quadrants of the posterior retina from right eyes of rcs rats at p21 ( a ) , p30 ( b ) , p45 ( c ) , and p60 ( d ) .
the hrl correlates with the pr / debris layer indicating a composition of is , disorganized os , and debris ( arrowheads ) .
spectral - domain oct allowed for high - resolution imaging of rcs retinal structure and quantification of retinal layers including tr , inl , onl+ , rpe , and hrl .
total retina and onl+ thickness degenerated over time , and the loss of the onl+ accounted primarily for tr reduction ( fig .
thus , the comprehensive tr and onl+ thickness measurements can be used as a baseline for future studies that assess the efficacy of potential therapies for outer retinal degeneration in the rcs rat .
in addition , hrl thickness , which correlated to is , disorganized os , and debris accumulation observed in histology ( fig .
when comparing our sd - oct analysis to previous published anatomic measurements , we found that thickness values differed , but the rate of thickness changes was consistent .
for example , both sd - oct and anatomic assessments show that half the onl thickness is decreased by approximately 7 weeks of age , that onl thinning occurs at a uniform rate in all four quadrants , and that the onl becomes absent in many areas of the posterior retina . however , manual segmentation of the onl+ resulted in thicker measurements than in previous reports , which measured distinctly around photoreceptor cell bodies ( e.g. , sd - oct at p17 : onl+ 90 m , tissue at p17 : onl 50 m ) .
this difference seems reasonable for two main reasons : ( 1 ) histologic processing is known to dehydrate the tissue ; and ( 2 ) our reported onl+ measurement included opl thickness , whereas onl measurements from previous anatomic reports do not include opl thickness .
hyperreflective layer thickness , acquired from sd - oct images , and an outer segment layer ( osl ) thickness , acquired from previous anatomic assessments , were also comparable . the osl has been quantified in order to estimate the relative volume of os debris accumulation during photoreceptor degeneration . in a previous anatomic assessment ,
it is reported that the osl thickness increases with age , but after maximum thickness is reached at p35 , the osl decreases with progressive degeneration .
these results seem comparable to the hrl identified in the rcs retina with sd - oct .
hyperreflective layer thickness then decreased with progressive degeneration and was consistently thicker in the superior retina .
quantification of the hrl in sd - oct images resulted in thicker values than in the quantification of the osl in retinal sections ( e.g. , sd - oct at p37 superior : hrl 61 m , tissue at p35 superior : osl / debris layer 48 m ) .
two main reasons could account for this discrepancy : ( 1 ) the osl measured on retinal sections may not include the inner segment thickness ; and ( 2 ) as the hyperreflectivity increased and the olm became less distinct , it is possible that the hrl may include cells from the onl .
overall , the hrl correlated with the pr / debris layer in our histology images ( fig .
4 ) , thus we suggest the hrl can be used to quantify photoreceptor inner and outer segment changes longitudinally
. many histologic changes could be responsible for generating the hrl , including disorganization of os discs resulting in lamellar whorls of membranes , the expansion or swelling of apical rpe processes , or invasion of macrophages into the outer retina .
our data demonstrated a correlation between preservation of onl+ thickness and increased hrl thickness in the superior retina from p30 to p37 ( supplementary table s2 ) , which supports this hypothesis .
histologic analysis of retinal tissue has been considered the gold standard for characterizing retinal degeneration and assessing the efficacy of potential treatments for amd and inherited retinal dystrophies in the rcs rat .
however , histologic processing precludes the ability to perform longitudinal analysis , thus increasing the number of animals required for long - term studies .
in addition , artifacts from postmortem processing are unavoidable , including fixation and dehydration , which can alter layer thicknesses and mask features of in vivo disease progression .
noninvasive , high - resolution sd - oct imaging offers an alternative in vivo approach to obtaining structural measurements of disease progression .
spectral - domain oct imaging has been validated in multiple mouse ( rho , rpe65 , rd1 , rd10 ) and rat ( rho p23h ) inherited retinal degeneration models . these studies have consistently reported that sd - oct is able to capture morphologic changes during disease progression and that sd - oct thickness measurements are notably higher , but in strong correlation with anatomic thickness measurements .
in addition , sd - oct has been able to image well - characterized phenotypes , as in the complete absence of rod os in the rho mouse , and reveal novel in vivo findings that lead to discoveries about disease progression .
for example , rpe65 mice have less distinct laminar organization , leading to the conclusion that expression of cytoskeletal elements and components of the extracellular matrix may be modified and playing a role in disease progression . in a study that characterized and revealed differences in disease progression between the rd1 and rd10 mice , retinal separations , often thought to be an artifact of histologic processing , were observed in the rd10 mice by sd - oct , confirming their in vivo existence . because of these findings , multiple studies now use retinal thickness measurements obtained from sd - oct images to demonstrate the long - term efficacy of various adeno - associated virus gene therapies for various inherited retinal degeneration mouse models ( rd1 , rd10 , p23h rho , rs1h , rd12 ) . despite the existing validation of sd - oct and numerous software programs available for segmentation of the retina ,
onl counts from h&e - stained retinal sections continue to be the main methodology used to illustrate preservation of photoreceptors after therapeutic interventions in the rcs rat .
the congruency between histologic analysis and sd - oct analysis demonstrated in this study suggests that sd - oct may also be used to quantify the efficacy of various therapies longitudinally .
we demonstrated that sd - oct overcomes the challenge of longitudinal anatomic assessments ; however , some limitations of this technology remain . the sd - oct analysis performed in this study
was restricted to the posterior retina ( up to 40 from the optic nerve head ) .
as photoreceptor degeneration rates differ between posterior and peripheral retina in the rcs rat , this study should be used primarily as a reference for interpreting data from the posterior retina , while recognizing that rates of degeneration can differ in the peripheral retina .
although sd - oct offers high - resolution imaging , manual segmentation of the onl became challenging during the course of degeneration .
the onl became hyperreflective over time , slowly becoming isoreflective with the opl , olm , and hrl , which made it difficult to distinguish . to address this limitation
however , since the olm became difficult to discern , this did not account for any onl that may have been masked by the hrl .
although the rpe was observable in sd - oct images , the segmentation analysis showed appreciable variation between the animals .
the outline of the rpe was clear in some images and less distinguished in others owing to hyperreflectance of the sclera and hrl .
it is well known that the rpe is maintained in the rcs rat , but we hypothesized that due to rpe dysfunction there may be measurable changes in its thickness .
our analysis suggests that if rpe thickness is changing during photoreceptor degeneration , better resolution imaging would be required to get consistent measurements of such a thin monolayer of cells .
the rcs rat has been and will continue to be a fundamental model used to develop therapies for amd and inherited retinal dystrophies .
we used sd - oct to quantify thickness of retinal layers in the rcs rat throughout the course of degeneration .
spectral - domain oct is an important complement to histology and in many cases could be used as a surrogate when assessing the efficacy of treatments for outer retinal degeneration in the rcs rat .
 purposeprospective treatments for age - related macular degeneration and inherited retinal degenerations are commonly evaluated in the royal college of surgeons ( rcs ) rat before translation into clinical application . 
 historically , retinal thickness obtained through postmortem anatomic assessments has been a key outcome measure ; however , utility of this measurement is limited because it precludes the ability to perform longitudinal studies . to overcome this limitation , the present study was designed to provide a baseline longitudinal quantification of retinal thickness in the rcs rat by using spectral - domain optical coherence tomography ( sd - oct).methodshorizontal and vertical linear sd - oct scans centered on the optic nerve 
 were captured from long - evans control rats at p30 , p60 , p90 and from rcs rats between p17 and p90 . 
 total retina ( tr ) , outer nuclear layer+ ( onl+ ) , inner nuclear layer ( inl ) , and retinal pigment epithelium ( rpe ) thicknesses were quantified . 
 histologic sections of rcs retina obtained from p21 to p60 were compared to sd - oct images.resultsin rcs rats , tr and onl+ thickness decreased significantly as compared to long - evans controls . 
 changes in inl and rpe thickness were not significantly different between control and rcs retinas . from p30 to p90 a subretinal hyperreflective layer ( hrl ) was observed and quantified in rcs rats . 
 after correlation with histology , the hrl was identified as disorganized outer segments and the location of accumulated debris.conclusionsretinal layer thickness can be quantified longitudinally throughout the course of retinal degeneration in the rcs rat by using sd - oct . 
 thickness measurements obtained with sd - oct were consistent with previous anatomic thickness assessments . 
 this study provides baseline data for future longitudinal assessment of therapeutic agents in the rcs rat . 
Methods
Animals
Imaging
Image Processing and Segmentation
Histology
Results
Qualitative SD-OCT Analysis
Quantitative SD-OCT Analysis
Histology and SD-OCT Image Comparison
Discussion
Supplementary Material