
 toxoplasma gondii ( t. gondii ) is an obligate intracellular protozoan parasite that causes toxoplasmosis , a usually asymptomatic disease in most immune - competent individuals but potentially severe in immune - compromised hosts .
successful control of this pathogen depends on the early production of the proinflammatory cytokines il-12 and ifn- , required for the development of a protective cell - mediated immune response against the parasite [ 25 ] .
although neutrophils and macrophages produce il-12 in response to t. gondii infection or its soluble antigens ( stag ) [ 69 ] , in vivo studies indicate that cd8 and cd11b dendritic cell ( dc ) populations may be the most important sources of this proinflammatory cytokine during acute infection [ 1012 ] .
the strong il-12-driven response against t. gondii results in the systemic production of ifn- by natural killer ( nk ) cd4 and cd8 t cells , which promotes the effector mechanisms to control both acute and chronic infections [ 2 , 1318 ] .
mif is a pleiotropic inflammatory cytokine that plays a role in the outcome of a variety of protozoan infectious diseases [ 1923 ] .
we have previously demonstrated that balb / c mif mice were more susceptible to t. gondii infection , with the virulent rh strain and the avirulent me49 strain .
the increased susceptibility in mif mice was associated with deficient serum levels of inflammatory cytokines ( il-12 and ifn- ) , as well as impaired il-12 production and reduced expression of costimulatory molecules in cd11c cells from spleen or mesenteric lymph nodes .
these data indicated a deficient innate immune response on infected mif mice presumably due to a deficient dc maturation .
however , as macrophages are able to express cd11c   and different dcs subsets share the expression of this marker , it is difficult to discern whether mif has a role in the maturation of dc subsets involved in the resistance to t. gondii . therefore , the present study aimed to assess the role of mif in the maturation of conventional cd8 and cd11b dcs during the acute infection with avirulent t. gondii me49 strain .
we demonstrate that serum mif increased rapidly during the acute infection with t. gondii and promoted maturation of cd11b but not cd8 dcs .
adoptive transfer of bone marrow ly6c monocytes suggested that systemic mif levels are required for conversion of this monocyte subset into tipdcs .
mif mice displayed reduced ifn- natural killer ( nk ) cells compared to infected wt mice , suggesting that mif promotes the recruitment of ifn--producing nk cells . finally , administration of rmif to t. gondii - infected mif mice restored the numbers of tipdcs and inflammatory cytokines along with the resistance to the parasite .
our results uncover a new role for mif in the development of cell - mediated immunity to t. gondii by favoring tipdc differentiation during acute toxoplasmosis .
all experiments in this study were performed strictly according to the guidelines for the care and use of laboratory animals adopted by the us national institutes of health and the mexican regulation of animal care ( nom-062-zoo-1999 , 2001 ) .
protocols were also approved by the institutional animal care and use committee ( iacuc ) , the research institute at nationwide children 's hospital .
adult 6- to 8-week - old female mice ( balb / c background ) were purchased from harlan laboratory ( mxico or usa ) .
mif mice were developed as previously described   and backcrossed for more than 10 generations to a balb / c genetic background .
all animals were maintained in a pathogen - free environment and established as breeding colonies in the animal facility at fes - iztacala , universidad nacional autnoma de mxico ( unam ) , or in the transgenic mouse facility at the research institute at nationwide children 's hospital animal facility .
cysts of the avirulent t. gondii me49 strain were harvested from brains of c57bl/6 mice that had been inoculated i.p . with 40 cysts 2 months earlier . for experimental infections , mif or wt mice received via intraperitoneal ( i.p . ) 100 cysts or pbs as a control .
control inoculations with uninfected brains failed to elicit detectable inflammatory responses or significant increases in cytokine levels . to assess acute disease progression ,
mice were killed by co2 and the peritoneal fluids were recovered under sterile condition using 3  ml of warm pbs and gentle massage at days 5 and 7 after toxoplasma infection of mif and wt mice .
tachyzoites were counted by trypan blue exclusion with a neubauer hemocytometer under olympus bx51 microscope ( 100 ; olympus american , melville , ny ) equipped with a digital video camera .
serum was obtained from infected or uninfected mif and wt mice during the acute phase of infection with t. gondii ( 1 , 2 , 3 , 5 , and 7 days after infection ) .
the whole blood was centrifuged for 10  min at 2000  g at 4c .
mif levels were determined by elisa according to the manufacturer 's guidelines ( neobiolab ) .
serum ifn- , il-6 , il-12p70 , il-4 , and il-10 were measured using a bio - plex pro assay ( bio - rad ) or cytometric bead array ( cba th1/th2 , bd biosciences ) according to the manufacturer 's protocols .
single - cell suspensions were obtained from peritoneal exudate cells ( pecs ) by peritoneal wash with 5  ml ice - cold pbs .
pecs pellets were washed twice with complete rpmi 1640 media and filtered with 70  m nylon mesh ( fisher scientific ) .
two milliliters of ack lysing buffer ( gibco , life technologies ) was added to lyse red blood cells .
harvested cells were incubated in saturated doses of anti - mouse fc receptor in 100  l of ice - cold facs buffer ( 1% bovine serum albumin/0.01% nan3 in pbs ) for 15  min .
after washing , 3  10 cells were stained with various combinations of antibodies in ice - cold facs buffer for 15  min and further collected on a lsr ii cytofluorometer ( becton dickinson , bd ) .
cells were gated according to size ( ssc - a ) and forward scatter ( fsc - a ) .
blue - fluorescent reactive dye l23105 ( life technologies ) was used to discard dead cells .
absolute cell numbers were calculated with the total cell count multiplied successively by the percentages for the appropriate gates obtained through flow cytometry .
peritoneal cells free of red blood cells were washed with complete rpmi 1640 media and adjusted 3  10  cells / ml . two ml per well of the cell suspension was incubated in 24-well plate , stimulated with 5  ng / ml of phorbol 12-myristate 13-acetate ( pma ) ( sigma ) plus 500  ng / ml of ionomycin ( sigma ) and 10  g / ml of brefeldin - a ( biolegend ) at 37c , 5% co2 for 4 hours .
cells were recovered and placed into facs tubes containing 1  ml of ice - cold facs buffer and centrifuged at 1500  rpm for 5  min . after surface staining ,
cells were washed and fixed using 2% paraformaldehyde in pbs for 10  min , rt , darkness .
cells were washed with intracellular staining buffer ( pbs , 1% fcs , and 0.025% saponin ) and resuspended with 100  l of the same buffer containing the indicated mabs for cytokines for 10  mins , rt , darkness .
cells were washed , resuspended in 500  l of buffer facs , and analyzed in facsaria fusion cytometer ( becton dickinson , bd ) .
the following mabs were used : anti - cd11c alexa fluor 700 ( n418 ) or anti - dec-205 pe - cy7 ( 205yekta ) , anti - mhcii efluor 450 ( m5/114.15.2 ) , anti - cd40 pe ( 1c10 ) , anti - cd86 fitc ( ct - cd8b ) , anti - cd86 brilliant violet 605 ( gl1 ) , anti - cd11b alexa fluor 647 ( m1/70 ) , anti - ly6c alexa fluor 488 ( hk1.4 ) , anti - f480 alexa fluor 488 ( bm8 ) , anti - ly6c efluor 450 ( hk1.4 ) , and anti - inos pe ( cxnft ) from ebioscience ; anti - cd8 alexa fluor 700 or percp ( 53 - 6.7 ) , anti - cd11b alexa fluor 700 ( m1/70 ) , anti - f4/80 brilliant violet 605 ( bm8 ) , anti - ly6 g percp ( 1a8 ) , anti - cxcr4 brilliant violet 421 ( 1276f12 ) , anti - cxcr2 pe / cy7 ( 5e8/cxcr2 ) , anti - tnf- brilliant violet 650 ( mp6-xt22 ) , anti - cd3 fitc ( 145 - 2c11 ) , anti - cd49 apc ( dx5 ) , and anti - ifn- pecy7 ( xmg1.2 ) from biolegend ; and anti - mhc - ii pe ( af6 - 120.1 ) , anti - cd8 percp ( 53 - 6 ) , anti - cd11c apc ( n418 ) , anti - cd74 fitc ( in-1 ) , anti - il-12p40/70 v450 ( c15.6 ) , and anti - il-12p40/70 apc ( c15.6 ) from bd bioscience .
, bone marrow cell suspensions were isolated by flushing femurs and tibias from 6- to 8-week - old mif or wt balb / c mice with complete rpmi 1640 supplemented with 10% fetal calf serum ( fcs ) , 100 units of penicillin / streptomycin , 2  mm glutamine , 25  mm hepes buffer , and 1% nonessential amino acids ( all from gibco , brl grand island , ny , usa ) .
bone marrow monocyte cells ( bm - mncs ) were isolated using a density - gradient centrifugation method .
bm - mncs were incubated with a mixture of antibody microbeads according to the manufacturer 's protocol ( miltenyi biotec , auburn , ca ) .
the negative fraction was collected ( putative monocytes ) and stained for cell sorting with a bd influx sorter .
isolated populations of ly6c monocytes were obtained and stained with the cell trace proliferation dye efluor 450 ( cat .
number 65 - 0842 - 85 ; ebioscience ) and transferred ( 2  10 cells ) intravenously ( i.v . ) to t. gondii - infected or uninfected mif or wt balb / c mice .
a group of 13 mif and 13 wt mice were infected via i.p . with 100 cysts of t. gondii .
mice received ( via i.p . ) 0.5  g per mouse of rmif ( biolegend # 599504 ) daily during the first 5 days after t. gondii infection .
three mice of this group were selected randomly and sacrificed on days 3 and 5 after infection to analyze the presence of tipdcs and production of serum ifn- ( as previously described ) .
uninfected mice that did not receive rmif were used as a control . the rest of the group , 7 mif and 7 wt mice , were monitored until day 25 to register the mortality and number of cysts per brain .
the total number of cysts was determined by counting cysts under olympus bx51 microscope ( 100 ; olympus american , melville , ny ) equipped with a digital video camera ( 40x ) in a 10  l aliquot and multiplied by 200 .
statistical comparisons were performed by either student 's t - test or one - way anova followed by tukey 's multiple comparisons for data that had a normal distribution .
although mif plays a critical role in the resistance to t. gondii infection , it is not known whether this protective role starts during the acute phase of infection with the parasite .
therefore , we first evaluated the systemic levels of inflammatory cytokines from acutely infected wt and mif mice with the avirulent me49 strain from t. gondii .
as shown in figure 1(a ) , serum mif from infected wt mice increased as early as 1 day after infection , peaked at 3 and 5 days , and decreased at day 7 .
the absence of mif led to deficient levels of il-12p70 , ifn- , il-6 , and tnf- in sera ( figures 1(b)1(e ) ) , but the anti - inflammatory cytokine il-10 was similar between groups ( figure 1(f ) ) .
these data suggest that the early upregulation of mif favors the prompt inflammatory immune response to acute toxoplasmosis . under conditions of infection ,
dcs mature by exposure to microbial agents or inflammatory mediators and increase their antigen processing capacity as well as their expression of costimulatory molecules and cytokine production .
recent studies in our laboratory suggest a role for mif in inducing dc maturation from spleen or mesenteric lymph nodes during experimental toxoplasmosis .
because dcs are essential for resistance to acute toxoplasmosis [ 1012 , 31 ] , we evaluated the role of mif in the maturation of conventional cd8 and cd11b dc subsets in peritoneal exudate cells as the early primary response in the i.p .
t. gondii infection . in figure 2(a ) , we show the gating strategy used for identification of cd11b ( cd11c mhcii cd11b ) and cd8 dcs ( cd11c mhcii dec205 cd8 ) subsets .
the absolute numbers of both cd11b ( figure 2(b ) ) and cd8 ( figure 2(c ) ) dcs increased at the site of infection ( peritoneal cavity ) until reaching a peak by day 5 after infection . comparable numbers of these cell populations were observed between wt and mif mice .
nevertheless , mif mice displayed fewer il-12p70 cd11b dcs ( figure 2(d ) ) , compared to wt mice at 3 ( p = 0.0028 ) and 5 ( p = 0.0006 ) days after infection . similar numbers of il-12p70 cd8 dcs were observed between mif and wt infected mice ( figure 2(e ) ) .
in addition , mif cd11b dcs displayed reduced cd86 expression ( figures 3(a ) and 3(b ) ) at 3 ( p = 0.0498 ) and 5 ( p = 0.0194 ) days after infection , but no differences were observed in their expression of cd40 ( figure 3(d ) ) . on the other hand ,
the expression of costimulatory molecules in cd8 dcs was unaltered in the absence of mif ( figures 3(a ) , 3(c ) , and 3(e ) ) .
similar results were observed on dcs from the spleen ( figure s-1 in supplementary material available online at http://dx.doi.org/10.1155/2016/9101762 ) .
these findings suggest a role for mif in selectively inducing maturation of cd11b dcs , a population that has proven to be crucial in the control of parasite replication and induction of the adaptive immune response against t. gondii .
previous studies have demonstrated that both inos and tnf- are essential factors in the control of t. gondii infection .
inos - deficient mice succumb during the chronic stage of infection with t. gondii , due to uncontrolled parasite replication , while tnf-r - deficient mice are susceptible to both acute and chronic toxoplasmic encephalitis [ 4 , 3235 ] .
monocyte - derived tnf-/inos - producing cd11b dcs ( tipdcs ) can be induced during parasitic or microbial infections , and they exert potent antimicrobial functions required to control the infection [ 3639 ] .
however , the role of mif in inducing tipdcs during acute t. gondii infection has not been addressed .
therefore , using the same strategy for identification of cd11b ( cd11c mhcii cd11b ) and cd8 ( cd11c mhcii dec205 cd8 ) dcs described above , we determined tnf- and inos production on cd11b and cd8 dcs from infected wt and mif mice .
our data revealed a population of tipdcs that gradually increased in both wt and mif mice during the acute phase of infection ( figure 4(a ) ) .
however , infected mif mice displayed reduced numbers of tipdcs ( p = 0.0049 ; figures 4(a ) and 4(b ) ) and tnf- inos cd11b ( p = 0.0090 , figures 4(a ) and 4(c ) ) dcs at 5 days after infection .
no differences were observed in inos tnf- cd11b dcs ( figures 4(a ) and 4(d ) ) .
cd8 dcs showed a gradual increase in tnf- ( figures 4(e)4(h ) ) , but not inos ( figures 4(f ) and 4(h ) ) , production during acute t. gondii infection .
comparable numbers of tnf--producing cd8 dcs were observed between wt and mif mice ( figure 4(g ) ) .
these findings demonstrate a novel role for mif in inducing tipdcs during acute toxoplasmosis and the ability of cd11b but not cd8 dcs to mature into tipdcs .
the chemokine - like inflammatory mediator mif is a ligand for the single - pass type ii membrane protein cd74   and a noncognate ligand for the chemokine receptors cxcr2 , cxcr4 , and cxcr7 .
because the role of mif in promoting dc maturation was only observed on cd11b dcs , we determined whether this phenomenon was associated with a differential expression of mif receptors on cd11b and cd8 dcs from infected wt mice . in basal conditions ,
wt cd11b and cd8 dcs show similar expression of cd74 , cxcr4 , and cxcr2 ( figures 5(a ) , 5(b ) , and 5(c ) ; day 0 ) .
however , during the course of t. gondii infection , wt cd11b dcs express more intensively the receptors cd74 ( 3 days , p = 0.0013 ; 5 days , p = 0.0014 ; figure 5(b ) ) , cxcr4 ( 5 days , p = 0.0014 ; figure 5(c ) ) , and cxcr2 ( 3 days , p = 0.0241 ; 5 days , p = 0.0341 ; figure 5(d ) ) , compared to wt cd8 dcs ( figures 5(a)5(d ) ) .
these results suggest that the selective role of mif in the maturation of cd11b dcs may be related to the high expression of mif receptors on this dc subset .
two major subsets of circulating monocytes have been characterized in mice based on their phenotypical and functional characteristics .
one subset expresses a high level of ly6c and is referred to as inflammatory monocytes due to its early response and selective migration into inflamed tissues [ 42 , 43 ] . the second subset , termed 
resident  or  patrolling  monocytes , expresses a low level of ly6c and is primarily distributed in blood vessels and tissues under the steady state , where it provides surveillance functions [ 4245 ] . because inflammatory ly6c monocytes are able to differentiate into tipdcs [ 36 , 39 ] and newly recruited ly6c monocytes
infection with t. gondii [ 4650 ] , we analyzed the activation of ly6c and ly6c monocytes in acutely infected mif and wt mice .
we found that resident ly6c monocytes and f4/80 macrophages ( ms ) patrolled the peritoneal cavity in basal conditions ( figure s-2b , day 0 ) .
however , upon infection with t. gondii , newly recruited monocytes ( ly6c and ly6c ) populated the peritoneal cavity at 3 and 5 days after infection , and few or no resident ms remained ( figure s-2b , 3 and 5 days after infection ) .
no significant differences were observed in the absolute numbers of monocytes or resident ms between infected mif and wt mice ( figure s2c - e ) .  
figure 6 shows that mif  ly6c monocytes had reduced numbers of both tnf- inos ( p = 0.0330 ; figures 6(a ) and 6(b ) ) and inos tnf- ( p = 0.0473 ; figures 6(a ) and 6(c ) ) cells , when compared to wt ly6c monocytes .
no differences were observed in ly6c tnf- inos monocytes between mif and wt infected mice ( figures 6(a ) and 6(d ) ) .
interestingly , the ly6c subset ( figure 6(e ) ) showed no differences between mif and wt infected mice in tnf- inos ( figure 6(f ) ) , inos tnf- ( figure 6(g ) ) , and tnf- inos monocytes .
these data suggest that mif promotes the activation of inflammatory ly6c monocytes and possibly their differentiation into tipdcs during acute t. gondii infection .
to address whether mif influences differentiation of inflammatory ly6c monocytes into tipdcs during the acute t. gondii infection , we determined the fate of adoptively transferred bone marrow ly6c monocytes from mif or wt mice ( donors ) into t. gondii - infected ( recipients ) mif or wt mice ( figure s3 ) .
we first analyzed the numbers of transferred ly6c monocytes differentiated into cd11b dcs ( cd11c mhcii cd11b cell trace , figure 7(a ) ) in the peritoneal cavity .
no significant differences were observed in the total number of monocyte - derived cd11b dcs between wt and mif recipient mice after the transfer with wt or mif  ly6c monocytes ( figures 7(b ) and 7(c ) , resp . ) .
moreover , we sought the tipdcs subset in the population of cd11b dcs and found that tipdcs were missing in both healthy wt and mif recipient mice ( figures 7(a ) , 7(d ) , and 7(e ) , day 0 ) .
however , after 5 days of t. gondii infection , wt and mif recipient mice showed a subset of ly6c monocytes expressing markers for tipdcs ( figure 7(a ) , day 5 ) .
interestingly , wt and mif  ly6c monocytes displayed similar ability to differentiate into tipdcs after being transferred to wt recipient mice ( figures 7(d ) and 7(e ) , wt recipient mice ) .
however , both wt and mif  ly6c monocytes were less able to differentiate into tipdcs when transferred into recipient mif mice compared to recipient wt mice ( figure 7(d ) , p = 0.0049 ; figure 7(e ) , p = 0.0047 ) .
these results indicate that ly6c monocytes differentiate into cd11b dcs in mif - independent manner ( figures 7(b ) and 7(c ) ) , but endogenous mif levels could be required to induce maturation of monocyte - derived cd11b dcs into tipdcs during acute toxoplasmosis . on the other hand , ly6c monocytes displayed high expression of mif receptors cxcr4 ( figure s4 : ( a)-(b ) ) and cd74 ( figure s4 : ( c)-(d ) ) , compared to ly6c monocytes at 5 days after infection or resident ms ( f4/80 ) from uninfected mice , which could explain the selective role of mif in inducing activation and differentiation of ly6c monocytes into tipdcs .
no differences were observed in cxcr2 expression ( figure s4 : ( e ) and ( f ) ) .
early studies by goldszmid et al . have demonstrated that natural killer ( nk ) cell - derived ifn- is responsible for driving the differentiation of mononuclear phagocytes into macrophages and il-12 producing cd11b dcs during experimental toxoplasmosis . in this context , the role of mif in regulating nk cell - derived ifn- at the site of infection with t. gondii could be essential for the differentiation of ly6c monocytes into tipdcs . therefore ,
we analyzed the ifn- production in peritoneal nk cells from t. gondii - infected wt and mif mice ; in figure 8(a ) , we show the gating strategy used .
we observed that either wt or mif nk cells ( cd3 cd49b ) displayed low percentages of ifn- cells under steady state ( figure 8(b ) ) .
however , upon t. gondii infection , wt and mif nk cells ( cd3 cd49b ) drastically increased their ifn- production at 2 and 3 days after infection ( figure 8(b ) ) .
interestingly , mif nk cells showed a reduced percentage of ifn- cells , compared to wt nk cells ( figure 8(b ) ) .
this observation was in line with a higher number of tachyzoites observed in the peritoneum of mif compared with wt mice at days 5 and 7 after t. gondii infection ( figure s5 ) .
the data suggest that mif induces ifn--producing nk cells during the acute infection with t. gondii .
these findings could be related to a recent study in a murine model of skin inflammation , demonstrating a role for mif in inducing the recruitment of ifn--producing nkt cells .
therefore , mif could promote the migration and/or activation of nk cells during acute t. gondii infection , a crucial event in the differentiation of ly6c monocytes into tipdcs . because our results indicated that systemic mif levels are critical for resistance and induction of tipdcs during acute toxoplasmosis , we confirmed these data by administration ( via i.p . ) of rmif into mif mice ( 0.5  g per mouse )
the rmif administration fully abolished the susceptibility of infected mif mice , which reached a survival rate of 100% , whereas untreated mif mice displayed a survival rate of 40% ( p = 0.0114 ) ( figure 9(a ) ) . the enhanced resistance induced by rmif administration
was associated with decreased numbers of cysts in the brain from mif mice ( p = 0.0234 ; figure 9(b ) ) after 25 days after infection .
in addition , serum ifn- levels were significantly recovered in mif mice that received rmif compared to mif that did not receive rmif ( p = 0.0107 ; figure 9(c ) ) .
strikingly , rmif administration reconstituted the absolute numbers of tipdcs at 5 days after t. gondii infection ( figure 9(d ) ) . altogether , these data demonstrate that induced mif levels during acute toxoplasmosis have a crucial role in the resistance and the development of the protective cell - mediated immune response against t. gondii .
we have previously demonstrated that mif favors immunity to t. gondii by inducing inflammatory cytokines ( il-12 and ifn- ) essential to control both acute and chronic infections with this parasite .
moreover , mif has a role in inducing maturation of cd11c cells , which indicates that mif promotes cell - mediated immune responses against t. gondii infection .
however , it is not known whether this inflammatory mediator induces maturation of specific dcs subsets required to control acute toxoplasmosis .
cd8 and cd11b dcs have prominent roles as the major sources of the inflammatory cytokine il-12 , required for ifn--mediated resistance to t. gondii [ 1012 , 31 ] .
therefore , in the present study , we sought to determine the role of mif in the maturation of cd11b and cd8 dcs during acute t. gondii infection .
we found that mif was one of the first cytokines increased in serum from infected wt mice , and in agreement with previous studies , the lack of this inflammatory mediator reduced the levels of other inflammatory cytokines , such as il-12 , ifn- , and tnf- , with all of them involved in immunity against t. gondii [ 32 , 5355 ] .
interestingly , cd11b but not cd8 dcs from infected mif mice showed impaired maturation characterized by deficient il-12p70 production and low expression of the costimulatory molecule cd86 . therefore , as cd11b dcs have a crucial role in the control of acute toxoplasmosis , an important mechanism by which mif promotes resistance to t. gondii may be through inducing maturation of cd11b dcs .
additionally , cd11b dcs displayed high expression of mif receptors cxcr4 , cd74 , and cxcr2   compared to cd8 dcs , suggesting that cd11b dcs are more sensitive to paracrine / autocrine mif stimulus than cd8 dcs during acute toxoplasmosis .
parasitic or microbial infections with trypanosoma brucei [ 38 , 39 ] or listeria monocytogenes , respectively , induce potent inflammatory immune responses that generate inos / tnf--producing cd11b dcs ( tipdcs ) , which derive from inflammatory monocytes recruited in the site of infection .
t. gondii infection induces monocyte - derived il-12 producing cd11b dcs , which accumulate in the peritoneal cavity and have a crucial role in the protective immune response to the parasite .
nevertheless , the production of inos and tnf- , essential factors in the control of t. gondii infection [ 4 , 3235 ] , has not been addressed on this dc subset [ 32 , 49 , 57 , 58 ] . here
infection with t. gondii , a population of tipdcs gradually increased in the peritoneal cavity from infected wt and mif mice .
interestingly , infected mif mice displayed reduced numbers of tipdcs ( 5 days after infection ) compared to infected wt mice , indicating a novel role for mif in inducing tipdcs during experimental toxoplasmosis . however , it is important to note that although the term tipdcs has been widely accepted to designate inos / tnf--producing dcs , previous reports have argued that it does not necessarily refer to a distinct dc subset , but rather to common dcs responding to their environment with inos and tnf- production [ 36 , 37 ]
. therefore , as comparable numbers of cd11b dcs were detected in both infected mif and wt mice , but reduced numbers of tipdcs were observed in the absence of mif , these data suggest that mif induces maturation of cd11b dcs into tipdcs , rather than recruitment of tipdcs . furthermore , a small population of cd8 dcs was detected during acute t. gondii infection ( figure 4(b ) ) , corroborating a previous report .
cd8 dcs displayed mif - independent maturation , characterized by increased tnf- but not inos production , which also indicates that cd11b but not cd8 dcs are able to become tipdcs during acute toxoplasmosis .
previous studies have demonstrated that nk cell - derived ifn- is required for local differentiation of inflammatory monocytes into both macrophages and mature il-12 producing dcs .
in contrast , maturation of cd8 dcs does not require priming by ifn- [ 31 , 59 ] but is critically dependent on tlr stimuli .
interestingly , the role of mif inducing the recruitment of ifn--producing nkt cells in a murine model of psoriasis has been recently demonstrated .
therefore , we analyzed ifn--producing nk cells from t. gondii - infected wt and mif mice .
we found that t. gondii - infected mif mice displayed a lower percentage of ifn- nk cells compared to infected wt mice , which could alter the activation and local differentiation of inflammatory monocytes into tipdcs .
inflammatory monocytes are known as the main precursors of tipdcs during parasitic and microbial infections [ 36 , 39 ] .
a variety of studies indicate that ly6c monocytes are required to control cerebral toxoplasmosis , and depletion of inflammatory monocytes ( ly6c ) but not neutrophils ( ly6 g ) increases the susceptibility of c57bl/6 mice to oral t. gondii infection [ 4649 ] .
therefore , we analyzed the activation state and ability of inflammatory monocytes to differentiate into tipdcs during acute toxoplasmosis .
infection with t. gondii , newly recruited monocytes ( ly6c and ly6c ) populated the peritoneal cavity of infected mice in a mif - independent manner .
however , the absence of mif reduced the activation of ly6c monocytes as indicated by significant lower inos / tnf- production ( well - established markers for m1 polarization ) .
in contrast , this was not observed on ly6c monocytes , suggesting a role for mif in promoting the activation of ly6c monocytes into a m1 profile .
furthermore , adoptive transfer of bone marrow ly6c monocytes revealed that mif monocytes were as capable as wt monocytes of converting into tipdcs when transferred to infected wt mice .
however , this ability was reduced in both , wt and mif monocytes , when transferred into infected mif mice , demonstrating that the presence of mif in ly6c monocytes has a limited role in the conversion of this population into tipdcs , compared to systemic mif levels released / produced by other cell types during acute t. gondii infection .
finally , administration of rmif to infected mif mice over the first 5 days of infection with t. gondii fully restored the resistance of mif mice and reconstituted the absolute numbers of tipdcs as well as serum ifn- levels . enhanced resistance by rmif administration was associated with reduced parasite loads in brain , which indicated a better control of t. gondii infection .
our data demonstrate a novel role for mif promoting the resistance to t. gondii - infection by inducing classical activation of inflammatory monocytes ly6c and their conversion into tipdcs .
in addition , mif induced ifn--producing nk cells during the acute phase of infection with t. gondii , required for differentiation of inflammatory monocytes into tipdcs .
further experiments are required to detail the mechanism by which mif regulates ifn- production in nk cells .
the administration of rmif into mif mice fully restored the resistance to acute toxoplasmosis , which highlights the crucial role of this inflammatory mediator in the development of the protective cell - mediated immune response to t. gondii and indicates a potential use of rmif as a therapeutic tool on immune - compromised hosts to control toxoplasmosis and probably other protozoan infectious diseases .
 macrophage migration inhibitory factor ( mif ) mediates immunity against toxoplasma gondii infection by inducing inflammatory cytokines required to control the parasite replication . 
 however , the role of this inflammatory mediator in the cell - mediated immune response against this infection is still poorly understood . here , we used t. gondii - infected wt and mif/ mice to analyze the role of mif in the maturation of cd11b+ and cd8+ dendritic cells ( dcs ) . 
 we found that mif promotes maturation of cd11b+ but not cd8+ dcs , by inducing il-12p70 production and cd86 expression . 
 infected mif/ mice showed significantly lower numbers of tnf and inducible nitric oxide synthase- ( inos- ) producing dcs ( tipdcs ) compared to infected wt mice . 
 the adoptive transfer of ly6chigh monocytes into infected wt or mif/ mice demonstrated that mif participates in the differentiation of ly6chigh monocytes into tipdcs . 
 in addition , infected mif/ mice display a lower percentage of ifn--producing natural killer ( nk ) cells compared to wt mice , which is associated with reducing numbers of tipdcs in mif/ mice . 
 furthermore , administration of recombinant mif ( rmif ) into t. gondii - infected mif/ mice restored the numbers of tipdcs and reversed the susceptible phenotype of mif/ mice . 
 collectively , these results demonstrate an important role for mif inducing cell - mediated immunity to t. gondii infection . 
1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions