deranged hematological parameters , including thrombocytopenia , are features of human immunodeficiency virus ( hiv ) infection.1 thrombocytopenia , defined by platelet cell count of less than 15010/l,2,3 occurs in about 4%24% of hiv - infected cases.4,5 mechanisms for thrombocytopenia , such as platelet involvement in immune responses , cytopathic effect of antiretroviral therapy ( art ) regimens , and antigenic mimicry , are key in a setting of hiv.6,7 thrombocytopenia has been linked to adverse sequelae and is regarded as an independent predictor of morbidity and mortality among the hiv - infected group , owing to increased risk of bleeding , which may occur in the mucous membranes , skin , soft tissue , and intracranial sites.8,9 the associated bleeding may cause death if it involves critical sites.10 to avert the risk of bleeding , platelet transfusion may be indicated ; however , in uganda , component transfusion is not readily available,11 which limits success of clinical interventions in an immunologically compromised population . although cytopenias have been widely reported in hiv infection , there is little data regarding prevalence , correlates , and etiologic association of hiv - related thrombocytopenia in uganda .
this study sought to determine hiv - related thrombocytopenia in a high - hiv / acquired immune deficiency syndrome ( aids ) study population ( southwest uganda ) .
the findings from this study will form a basis for management of complications that arise from thrombocytopenia among hiv clients in this setting .
these were hiv - positive adult males and females who had been enrolled for care at the immune suppressed syndrome ( iss ) clinic in mbarara , uganda .
we sought informed consent of patients with thrombocytopenia as found in their previous full blood count ( fbc ) results , and investigated the presence of antiplatelet antibodies .
for each sample , 3 ml of blood was collected into a plain vacutainer , allowed to clot , and centrifuged , to obtain hemolysis - free serum that was kept frozen at 80c at the epicentre mbarara research centre , mbarara , uganda .
we performed indirect monoclonal antibody - specific immobilization of platelet antigens ( maipa ) for 40 serum samples from thrombocytopenic hiv clients , to screen and identify antiplatelet antibodies .
antibody screening was done using platelets from a pool of six group o donors selected for their platelet genotype ( advanced practical diagnostics bvba , turnhout , belgium ) ; these were incubated with serum , and mouse monoclonal antibodies specific for platelet glycoproteins ia / iia , ib / ix , iib / iiia , and anti -2-microglobulin .
lysates were cleared by centrifugation and transferred to microplate wells precoated with goat anti - mouse immunoglobulin g ( igg ) .
the bound complex was detected using goat peroxidase - coupled anti - human igg and revealed by peroxidase substrate o - phenylenediamine .
the reaction was stopped using sulfuric acid , and absorbance was read at 492 nm .
all positive antibody screens were identified , using a standard six - cell genotyped panel , by similar or additional methods .
a participant was regarded to have antiplatelet antibodies if one or more platelet antiglycoproteins were identified .
retrospective data from the iss database and data from laboratory analyses were entered into a statistical software package of excel 5.0 ( microsoft corp , redmond , wa , usa ) and transferred to stata 12 ( statacorp lp , college station , tx , usa ) to carry out data management and analysis , respectively .
we sought clients informed consent , and the study was approved by the institutional review committee of mbarara university of science and technology , and the uganda national council for science and technology .
these were hiv - positive adult males and females who had been enrolled for care at the immune suppressed syndrome ( iss ) clinic in mbarara , uganda .
we sought informed consent of patients with thrombocytopenia as found in their previous full blood count ( fbc ) results , and investigated the presence of antiplatelet antibodies .
blood was drawn , with minimal stasis , from the antecubital vein . for each sample ,
3 ml of blood was collected into a plain vacutainer , allowed to clot , and centrifuged , to obtain hemolysis - free serum that was kept frozen at 80c at the epicentre mbarara research centre , mbarara , uganda .
we performed indirect monoclonal antibody - specific immobilization of platelet antigens ( maipa ) for 40 serum samples from thrombocytopenic hiv clients , to screen and identify antiplatelet antibodies .
antibody screening was done using platelets from a pool of six group o donors selected for their platelet genotype ( advanced practical diagnostics bvba , turnhout , belgium ) ; these were incubated with serum , and mouse monoclonal antibodies specific for platelet glycoproteins ia / iia , ib / ix , iib / iiia , and anti -2-microglobulin .
lysates were cleared by centrifugation and transferred to microplate wells precoated with goat anti - mouse immunoglobulin g ( igg ) .
the bound complex was detected using goat peroxidase - coupled anti - human igg and revealed by peroxidase substrate o - phenylenediamine .
the reaction was stopped using sulfuric acid , and absorbance was read at 492 nm .
all positive antibody screens were identified , using a standard six - cell genotyped panel , by similar or additional methods .
a participant was regarded to have antiplatelet antibodies if one or more platelet antiglycoproteins were identified .
retrospective data from the iss database and data from laboratory analyses were entered into a statistical software package of excel 5.0 ( microsoft corp , redmond , wa , usa ) and transferred to stata 12 ( statacorp lp , college station , tx , usa ) to carry out data management and analysis , respectively .
we sought clients informed consent , and the study was approved by the institutional review committee of mbarara university of science and technology , and the uganda national council for science and technology .
records for 15,030 hiv clients at enrollment into care at the mbarara regional referral hospital iss clinic were used .
participants had a median age of 35.0 ( range 1878 ; iqr 2842 ) years .
out of 15,030 participants , 2,617 had thrombocytopenia , giving an overall prevalence of 17.4% ( 95% confidence interval [ ci ] : 16.8%18.0% ) .
the prevalence of thrombocytopenia among hiv clients who were art - nave ( n=2,675 ) was 17.8% ( 95% ci : 17.1%18.4% ) , while that for clients who were on art for up to 6 months ( n=6 ) was 13.0% ( 95% ci : 0.3%21.9% ) .
we used univariate and multivariate analyses to determine association of thrombocytopenia with clinical stage , cd4 count , art , anemia , and leucopenia , which we found significant ( p<0.05 ) ( tables 1 and 2 ) .
of the 40 thrombocytopenic samples tested for anti - platelet antibodies , two ( 5.0% ) had glycoprotein iib / iiia and iia / ia .
in this study , the overall prevalence of hiv - related thrombocytopenia was 17.4% ( 95% ci : 16.8%18.0% ) ; this is comparable to the 20% reported in iran12 and 12.7% in ethiopia.13 this is attributed to hiv - induced hemopoiesis dysfunction and platelet immunologic involvement , which aggravates their destruction.8 the prevalence of thrombocytopenia among hiv - infected , art - nave clients was 17.8% ( 95% ci : 17.1%18.4% ) ; this is similar to the 16.1% reported in nigeria14 and 13.5% in rwanda15 but higher than the 8.3% reported in kampala , uganda16 and the 5.9% reported in ethiopia.17 the high prevalence of thrombocytopenia in this group is probably attributed to already established mechanisms of hiv - related thrombocytopenia.18,19 this study showed a positive effect of regimens containing the nucleoside analog reverse - transcriptase inhibitor , zidovudine ( azt ) , which is similar to other reports;17 thus exclusive use of prophylactic co - trimoxazole among the art - nave hiv clients may be an independent predictor of accelerated thrombocytopenia .
since we detected antiglycoproteins in one of the art - nave participants , it is likely that such antibodies cause thrombocytopenia.20 the prevalence of thrombocytopenia among hiv - infected clients who were on art for up to 6 months was 13.0% ( 95% ci : 0.3%21.9% ) ; this is much higher than the 4.1% reported in ethiopia.21 this is probably due to diminished use of azt regimens , in our study population , due to associated anemia.22 we analyzed for art combinations as a predictor of thrombocytopenia , and from this , we elucidate reduced thrombocytopenia in participants on azt regimens .
the observed prevalence may as well be attributed to factors linked to induced cytopenias that vary according to the participant s profile , the etiologic basis , and art option.23 the occurrence of antiglycoprotein in one of the participants in this group shows antiplatelet antibody involvement in the etiology.8 using univariate and multivariate analyses to relate the independent predictors of thrombocytopenia , diminished cd4 counts , other cytopenias , and enrollment on art showed significant association with the occurrence of thrombocytopenia in our study participants ( p<0.05 ) , which is consistent with findings reported by other studies in the region.16,21 this may be due to defective hemopoiesis and the effect of art , as previously reported.21,24 the study found a significant association of thrombocytopenia with other cytopenias in all hiv stages ( refer to table 3 ) .
this may be due to defective bone marrow , accelerated platelet destruction , and to azt- or stavudine - containing regimens.25 there was an increasing risk of thrombocytopenia with hiv infection progression , and this is similar to findings from other studies.17,26 this may be because thrombocytopenia is greater in advanced hiv infection.15 the antiplatelet antibody prevalence of 5.0% is comparable with the 9.4% found among hiv chilean population27 but lower than the 17.0% in the italian population.28 the higher anti - human platelet antigens ( hpas ) against iib / iiia than ia / iia is due to their close association with the chronic thrombocytopenic states associated with hiv.29 anti - hpa-5 , which targets ib / ix , was missing in our study participants , as these are linked to thrombocytopenia due to neonatal alloimmunization.30 antiplatelet antibodies were seen in mild and moderate thrombocytopenic clients , which implies that severe thrombocytopenia is not associated with the production of antiplatelet antibodies , as already reported.28 the key limitation of study was the small sample size of participants enrolled for antiplatelet antibody assay owing to time and financial constraints .
the authors recommend a larger study to be carried out and that as a basis of critical care and management , immune thrombocytopenia should be considered in our study population .
the key limitation of study was the small sample size of participants enrolled for antiplatelet antibody assay owing to time and financial constraints .
the authors recommend a larger study to be carried out and that as a basis of critical care and management , immune thrombocytopenia should be considered in our study population .
this study has observed a high prevalence of thrombocytopenia , especially in the art - nave population , and the occurrence of antiglycoproteins in our sample population .
these results indicate that there is a need to monitor platelet counts and to initiate platelet component transfusion among hiv thrombocytopenic clients , in our current blood banking practice .
 aims / objectiveswe aimed to determine the prevalence and correlates of thrombocytopenia among people living with human immunodeficiency virus ( hiv)/acquired immune deficiency syndrome ( aids ) and to assess occurrence of antiplatelet antibodies , among thrombocytopenic hiv clients at mbarara regional referral hospital , southwestern uganda.materials and methodsthis was a retrospective review of hematologic results at enrollment to hiv care from 2005 to 2013 . 
 the prevalence and correlates of thrombocytopenia were estimated based on the immune suppressed syndrome ( iss ) clinic electronic database . 
 a cross - sectional study determined the occurrence of antiplatelet antibodies , using the monoclonal antibody - specific immobilization of platelet antigens ( maipa ) technique.resultswe reviewed 15,030 client records . 
 the median age was 35.0 ( range 1878 ; interquartile range [ iqr ] 2842 ) years , and there were 63.2% ( n=9,500 ) females . 
 the overall prevalence of thrombocytopenia was 17.4% ( 95% confidence interval [ ci ] : 16.8%18.0% ) . 
 the prevalence of thrombocytopenia was 17.8% ( 95% ci : 17.1%18.4% ) among antiretroviral therapy ( art)-nave clients ( n=2,675 ) and was 13.0% ( 95% ci : 0.3%21.9% ) for clients who were on art ( n=6 ) . 
 the study found a significant association between thrombocytopenia and other cytopenias , cd4 counts , art , and deteriorating hiv stage ( p<0.05 ) . 
 two of the 40 participants ( 5.0% ) had antiplatelet antibodies.conclusionthis study has showed a high prevalence of hiv - related thrombocytopenia . 
 antiplatelet antibodies were found in 5.0% of hiv - infected thrombocytopenic participants . 
 our study shows a significant association of thrombocytopenia burden in a high - hiv study population ( southwest uganda ) ; therefore , there is need to monitor platelet counts and initiate platelet transfusion in our blood banking practices , to avert possible risks of bleeding . 
Introduction
Materials and methods
Study participants
Sample collection
Laboratory analysis
Statistical analysis
Ethical considerations
Results
Discussion
Limitation of the study
Recommendations
Conclusion