On March 6 2006 the storyline had Stratus telling James that they needed time apart from each other. They wrestled as a team at the March 18, 2006 Saturday Night's Main Event. James and Stratus defeated Candice Michelle and Victoria in the match. After the match, James tried to kiss Stratus. Stratus pushed her away and James' character made a complete heel turn. She attacked Stratus in the ring. James' character later said she would destroy Stratus. The two women wrestled each other at WrestleMania 22. James won the match to become the Women's Champion for her first time. During the match, even though James was playing the heel character, the fans liked her more than Stratus. The storyline ended at Backlash with a rematch after Stratus legitimately dislocated her shoulder when James threw her out of the ring. James lost the Women's Title on August 14 to Lita. During the match, Lita hit James with the title belt when Edge distracted the referee. This causes a face turn for James' on-screen character. When Stratus retired as champion, there was no longer a Women's Championship. WWE held a tournament to name a new champion. James was a part of that tournament. She defeated Victoria and Melina in the early parts of the tournament. She made it to the finals at Cyber Sunday. In the final match, she lost to Lita. At the 2006 Survivor Series James defeated Lita to win her second Women's Championship.