In 1567, Darnley was murdered. James Hepburn, 4th Earl of Bothwell was put on trial for the murder but found not guilty. He was a powerful Scottish nobleman who was loyal to Mary and hated Darnley. Mary then married him. This caused her problems, because Bothwell had many enemies and many still believed he had murdered Darnley. Mary's enemies forced her off the throne and made her young son king in her place. Mary was put in prison in Loch Leven Castle. She escaped and crossed the border into England, which was ruled by her cousin, Elizabeth, Queen of England.