The region was under the authority of the lords of Valangin. In the 15th and 16th centuries, a second wave of colonization came from the so-called "Clos de la Franchise" (the valleys of Le Locle and La Sagne). Agriculture was the main activity but the village remained small. In 1531 there were only about 35 people living there. The first church was built in 1528. By 1530, La Chaux-de-Fonds, like the rest of the Valangin lands, converted to the new Reformed faith. The Lord of Valanginian, René de Challant, fixed the boundaries of the parish in 1550. The church and parish provided a political structure and a small community of Valanginian citizens, free farmers and peasants grew up around the church. By 1615 there were 355 people living in the village. In 1616, the low and middle jurisdiction over La Chaux-de-Fonds moved to Le Locle and La Sagne, while the high court remained in Valanginian. The agriculture, supplemented by mills on the banks of the Doubs, continued to dominate. However, at the end of the 16th century, the city became an important crossroad between Neuchâtel, Franche-Comté and the Bishopric of Basel.