He was born on January 14, 1907 in the village of Chernyakhiv (now Kaharlyk district of Kyiv region). He graduated from the Kyiv Medical School (now the Kyiv Medical School). In 1928 he entered the Kharkiv Medical Institute. After graduating from the institute in 1932, he was enrolled in the graduate school of the Ukrainian Organ Therapeutic Institute in Kharkiv. In 1935, Komisarenko defended his dissertation and worked as a senior researcher at the Ukrainian Organ Therapeutic Institute, and two years later he was appointed director of the institute.