Stephen Hopkins died sometime between June 6, 1644 and July 17th of that year. He made his will on June 6, 1644 and requested that he be buried next to his deceased wife, Elizabeth. The inventory was taken on July 17, 1644 and besides his deceased wife, he mentions sons Giles and Caleb, daughter Constance, wife of Nicholas Snow, daughters Deborah, Damaris, Ruth and Elizabeth.