Based on the television series, the game is set in both Dexter's house and laboratory. The game's single-player is divided into four levels with each contain puzzling challenges that feature various inventions Dexter has created. Each level contains two minigames which the player must find and complete to advance to the next level. The game features a total of eight minigames which can be played alone or in a two-player mode, with one person playing as Dexter and the other as Mandark. The minigames include Dee Dee's Dance Off, Cootie Call, Up N Atom, and Dexter Dodge Ball. In each level, players can also unlock a different improved invention by collecting four blueprint pieces hidden in the level. The improved inventions make it easier to complete the minigames in that level.