Maria Magdalena of Austria or Maria Maddalena of Austria (7 October 1589 – 1 November 1631) was Grand Duchess of Tuscany from her husband's accession in 1609 until his death in 1621. With him, she had eight children, including a duchess of Parma, a grand duke of Tuscany, and an archduchess of Further Austria. She was the youngest daughter of Charles II, Archduke of Inner Austria, and his wife Maria Anna of Bavaria. During the minority of her son, Grand Duke Ferdinando II, she and her mother-in-law Christina of Lorraine acted as regents. She died in Padua.