The highway begins in Erldunda, a roadhouse and hotel, on the Stuart Highway (N87). The Stuart Highway connects Darwin in the north and Adelaide in the south and is therefore one of Australia's major highways. From Erldunda, the Lasseter Highway leads west along the southern side of the Basedow and Kernot Ranges. After about 110 km, next to the Kernot Range, there is the junction with the Luritja Highway (S3), which leads north to Kings Canyon. Another 25 km towards the southwest is a rest area that has a view of Mount Conner. A further 22 km away is the Curtin Springs, a cattle station with a roadhouse. A further 100 km west along the highway is the tourist town Yulara. After Yulara, the road meets the Great Central Road, a dirt road that leads into Western Australia through the Petermann Ranges and across the Gibson and Great Victoria Deserts.