Tyus continued to compete and won national championships in sprint events and a gold medal in the 200 m at the Pan-American Games. In 1968, she returned to the Olympics to defend her title in the 100 m. In the final, she set a new World Record of 11.08 seconds. She became the first woman to hold the Olympic 100 m title through a second Olympic Games. Tyus also finished sixth in the 200 m. She ran the final leg for the relay team and helped set a new World Record. Winning the relay was her third gold medal.