Eur J Clin Microbiol Infect Dis. 2009;28:569 573. doi: 10.1007/s10096-008-0672-6. 91. Vondracek M, Sartipy U, Aufwerber E, Julander I, Lindblom D, Westling K. 16S rDNA sequencing of valve tissue improves microbiological diag- nosis in surgically treated patients with infective endocarditis. J Infect. 2011;62:472 478. doi: 10.1016/j.jinf.2011.04.010. 92. Mar n M, Mu oz P, S nchez M, del Rosal M, Alcal L, Rodr guez- Cr ixems M, Bouza E; Group for the Management of Infective Endocarditis of the Gregorio Mara n Hospital. Molecular diagnosis of infective endocarditis by real-time broad-range polymerase chain reac- tion (PCR) and sequencing directly from heart valve tissue. Medicine (Baltimore). 2007;86:195 202. doi: 10.1097/MD.0b013e31811f44ec. 93. Gauduchon V, Chalabreysse L, Etienne J, C lard M, Benito Y, Lepidi H, Thivolet-B jui F, Vandenesch F. Molecular diagnosis of infective endo- carditis by PCR amplification and direct sequencing of DNA from valve tissue. J Clin Microbiol. 2003;41:763 766. 94. Wolff TY, Moser C, Bundgaard H, H iby N, Nielsen PH, Thomsen TR. Detection of microbial diversity in endocarditis using cultivation-inde- pendent molecular techniques. Scand J Infect Dis. 2011;43:857 869. doi: 10.3109/00365548.2011.598877. 95. Lang S, Watkin RW, Lambert PA, Littler WA, Elliott TS. Detection of bacterial DNA in cardiac vegetations by PCR after the completion of anti- microbial treatment for endocarditis. Clin Microbiol Infect. 2004;10:579 581. doi: 10.1111/j.1198-743X.2004.00821.x. 96. Sukocheva OA, Marmion BP, Storm PA, Lockhart M, Turra M, Graves S. Long-term persistence after acute Q fever of non-infective Coxiella bur- netii cell components, including antigens. QJM. 2010;103:847 863. doi: 10.1093/qjmed/hcq113. 97. Voldstedlund M, N rum Pedersen L, Baandrup U, Klaaborg KE, Fuursted K. Broad-range PCR and sequencing in routine diag- nosis of infective endocarditis. APMIS. 2008;116:190 198. doi: 10.1111/j.1600-0463.2008.00942.x. 98. Barbour SI, Louie EK, O Keefe JP. 