Introduction
============

Carotid atherosclerotic stenosis is one of the main risk factor for ischemic stroke and it contributes to \>20% of incidence of ischemic stroke ([@b1-etm-0-0-3360]). Ischemic stroke can be prevented by treating the carotid atherosclerotic stenosis. The carotid artery intima stripping off technique remains the gold standard for the treatment of carotid atherosclerotic stenosis ([@b1-etm-0-0-3360]). However, in recent years, the rapid development of neuroimaging techniques has led to carotid angioplasty with stenting (CAS) becoming a simple, effective and minimally invasive method used for the treatment of extracranial carotid artery stenosis and has considerable promise to become an alternative treatment to carotid endarterectomy (CEA).

A previous study evaluated the risk of perioperative stroke, myocardial infarction, and mortaltiy associated with CAS and thus a quantitative scoring system was established ([@b2-etm-0-0-3360]). However, that study focused on the assessment of high-risk patients with CEA and did not include factors that cannot be controlled, such as age, gender, history of cardiovascular disease, and characteristics of lesions. Another study showed that, CAS-associated complications such as vasospasm, low blood flow dynamics change, acute in-stent thrombosis, and plaque prolapse, increase the episodes of major adverse cardiac and cerebrovascular events (MACCE) ([@b3-etm-0-0-3360]).

In this context, the aim of the present study was to identify the independent risk factors for MACCE, and establish a model for risk scoring for CAS. Patients underwent CAS and were followed up perioperatively. By considering the baseline, disease characteristics, age, gender and postoperative complications.

Materials and methods
=====================

### Patients

A total of 403 subjects were included in the study. There were 298 males and 105 females, and the patient age range was 45--83 years, with an average of 66.73±7.03 years. The patients underwent CAS in the Department of Neurology, Daping Hospital affiliated to the Third Military Medical University (Chongqing, China) between January 2010 and June 2013 were included. The present study was approved by the Medical Ethics Committee of Daping Hospital affiliated to the Third Military Medical University. The purpose and methods of this study were explained to the patients or their family members and written informed consent was provided to undergo relevant examinations required for the current study. The carotid atherosclerotic stenosis was measured as described in the North American Symptomatic Carotid Endarterectomy Trial ([@b4-etm-0-0-3360]). Diagnosis was made using head and neck computed tomography (CT) angiography and cerebrovascular digital subtraction angiography (DSA). The diagnosed carotid atherosclerotic stenosis was stratified into symptomatic carotid atherosclerotic stenosis (≥50% stenosis) and asymptomatic atherosclerotic stenosis (≥70% stenosis).

### Inclusion and exclusion criteria

Inclusion criteria for the study were as described in the guidelines for carotid stenting ([@b5-etm-0-0-3360]): i) asymptomatic carotid atherosclerotic stenosis patients; ii) symptomatic carotid atherosclerotic stenosis patients; and iii) patients or their family members who expressed willingness to participate in the study and signed the informed consent.

Exclusion criteria for the present study were as described by Brott *et al* ([@b1-etm-0-0-3360]): i) patients with loss of ipsilateral brain function, and paralysis, who did not benefit sufficiently from CAS; ii) lesion being completely occluded and the length of the lesion being \>10 mm, and no blood flow observed at the distal DSA; iii) ipsilateral intracranial arteriovenous malformations or aneurysms with bleeding tendency; iv) the incidence of intracerebral hemorrhage in the previous 3 months, or large cerebral infarction (CI) in the previous 4 weeks or acute myocardial infarction in the previous 2 weeks; v) severe heart, liver and kidney dysfunction; vi) contrast agent allergy and other angiographic contraindication; vii) allergy to aspirin enteric-coated tablets, clopidogrel and other anti-platelet agents; viii) combination of serial lesion and anterior circulation, posterior circulation and multiple cerebral vascular stent implantations; and ix) serious sick sinus syndrome or atrioventricular block.

### Collection of relevant patient data

Information concerning gender, age, smoking status and whether the patients had diseases or disorders including hypertension, diabetes, hypercholesterolemia, coronary cardiopathy, transient ischemic attack (TIA), symptomatic carotid atherosclerosis stenosis and CI was collected from the medical record of the patients.

### Basis of diagnosis of disease or disorders

One week after patients were enrolled, blood samples were collected aseptically from the elbow vein in the morning and subjected to analyses of total cholesterol, low-density lipoprotein cholesterin and blood glucose.

### Diagnosis of hypertension

Diagnosis of hypertension was carried out in accordance with Lloyd-Jones *et al* ([@b6-etm-0-0-3360]). Hypertension was defined as systolic blood pressure (SBP) greater ≥140 mm Hg or diastolic blood pressure (DBP) greater ≥90 mm Hg, or receiving medication specifically for the indication of hypertension.

### Diabetes mellitus

The diagnostic criterion, i.e., diabetes mellitus, established by the American Diabetes Association ([@b7-etm-0-0-3360]) was adhered to in diagnosing diabetes among patients.

### Diagnosis of hypercholesterolemia

The National Cholesterol Education Program Adult Treatment Panel III ([@b8-etm-0-0-3360]) was adhered to in order to identify hypercholesterolemia among patients. Hypercholesterolemia was diagnosed as total serum cholesterolemia ≥5.20 mmol/l (or ≥200 mg/dl) or low-density lipoprotein cholesterol ≥3.40 mmol/l (or ≥130 mg/dl).

### Coronary cardiopathy

Coronary cardiopathy diagnosis was established by observing the clinical symptoms of patients and subjecting the patients to electrocardiogram (ECG), treadmill exercise, echocardiography, coronary CT angiography and coronary angiography.

### Acute myocardial infarction

The diagnosis of acute myocardial infarction was as described previously ([@b9-etm-0-0-3360]). The patients with an increased amount of troponin than normal value with any of the symptoms of myocardial ischemia were considered to have acute myocardial infarction. Myocardial ischemia symptoms are changes in ischemic ECG, pathological Q wave of ECG, abnormalities in ventricular wall movement or myocardial inactivation ([@b9-etm-0-0-3360]).

### Symptomatic and asymptomatic carotid atherosclerotic stenosis

Carotid atherosclerotic stenosis was considered symptomatic when the patients experienced TIA or CI in the previous 6 months ([@b4-etm-0-0-3360]). The carotid atherosclerotic stenosis was considered asymptomatic if it persisted without the incidence of TIA or stroke.

### Diagnosis of aortic arch calcification

Aortic arch calcification was diagnosed through chest radiography (Philips Digital Radiography, Utrecht, The Netherlands). If the aortic arch was visible and \>1 cm in size with funicular and curved calcification, then it was considered as aortic arch calcification ([@b10-etm-0-0-3360]), as confirmed by two expert radiologists.

### Carotid atherosclerotic plaque typing

Carotid atherosclerotic plaque typing was performed using head and neck CT angiography (Lightspeed 64 row spiral CT; GE Healthcare, Piscataway, NJ, USA, and Brilliance iCT, 256 row; Philips Healthcare, Cleveland, OH, USA). After a cervical plain scan, the scanned images were transferred to an AW4.2 processing workstation (64-slice CT) or EBW processing workstation (256iCT) and analysis of multiplanar reformation, maximum intensity projection, volume rendering, vessels was conducted and the carotid atherosclerotic plaque CT values were measured. If the CT was ≥120 Hounsfield units (Hu), then it was considered as calcified plaque ([@b11-etm-0-0-3360]), whereas ≤120 Hu was considered as non-calcified plaque. If the contrast agent diffused \>1 mm depth along the surface of the plaque in the arterial lumen, the plaques were considered ulcerative ([@b12-etm-0-0-3360]).

### Determination of aortic arch type

Cerebral angiography was used to identify the aortic arch type, carotid artery stenosis rate, the extent of lesion, carotid artery tortuosity and carotid artery occlusion. The total arterial diameter of the left common carotid artery was taken as a reference and the vertical distance from the top of the aortic arch to the opening of the innominate artery was measured. If the measured distance was similar to the common carotid artery, it was considered a type I aortic arch; if the distance was 1--2 times more than that of the common carotid artery, it was considered a type II aortic arch and if the distance was \>2 times that of the common carotid artery, it was considered a type III aortic arch ([@b13-etm-0-0-3360]).

### Determination of carotid stenosis rate and length of lesion

The carotid artery stenosis rate was calculated as described in NASCET ([@b4-etm-0-0-3360]). The carotid artery stenosis rate (%) was calculated as: (normal carotid artery diameter of stenosis - diameter of the most narrow section/diameter of the normal carotid artery in the stenosis) × 100. The degree of stenosis was divided into mild (stenosis rate 0--29%), moderate (stenosis rate 20--69%), severe (stenosis rate 70% and above) and occlusion (stenosis rate 100%) in which no blood flow was evident at the distal end of the occlusion. The length of the lesion was obtained by measuring the vertical scope of the plaque. If the plaque was continuous, the distance from the top of the plaque to the bottom thereof was taken to estimate the length of the lesion. If the plaque was discontinuous, the distance from the top to the bottom of each plaque was taken and a summation was made to estimate the total length of the lesion.

### Carotid artery tortuosity

Carotid artery tortuosity includes 'C'-type tortuosity, 'S'-type kinking and 'O'-type coiling. 'C'-type tortuosity is characterized by vascular wavy lines ([@b14-etm-0-0-3360]). 'S'-type kinking is characterized by vascular elongation and changed angle and 'O'-type coiling is characterized by excessive elongation of vascular tortuosity as 'O' configuration ([@b15-etm-0-0-3360],[@b16-etm-0-0-3360]).

### CAS

At least 3 days prior to surgery, clopidogrel (75 mg/l) and aspirin (100 mg-200 mg/l) were administered to the patients daily. Phenobarbital sodium (0.1 g) was administered to the patients intramuscularly 5 h prior to surgical resection. For 3 days after surgery, fasudil hydrochloride (30 mg) was administered to the patients in 250 ml of 0.9% saline.

Surgery was carried out by an experienced surgeon according to the standard procedure for carotid artery angioplasty and stenting ([@b17-etm-0-0-3360]). The patient was placed in a supine position, and after local anesthesia (1% lidocaine hydrochloride) a Seldinger puncture of the femoral artery was performed into the 8 F vessel sheath with intraoperative heparinization and additional heparin (1,000 units) was also added every 1 h. The 8 F femoral artery was replaced after cerebral angiography and CAS. The 8F guiding catheter that was exchanged for 300 mm 0.018 guiding wire was positioned in the common carotid artery. A distal protection device (DPD) was placed inside the distal internal carotid artery. The DPD comprised AngioGuard (Cordis, Miami Lakes, FL, USA) and Spider RX (ev3, Plymouth, MN, USA) and was used according to the blood vessel type. In cases of excessive vascular tortuosity, the DPD was not placed because it was unable to reach the distal internal carotid artery.

The diameter of general carotid and internal carotid arteries were measured prior to and after DPD implantation in patients who underwent balloon predilation or stenting. Subsequent to stenting, if the degree of improvement was not satisfactory (\>30%), balloon dilation was performed. Repeat radiography was performed to determine whether there was any vasospasm, vascular dissection or distal blood vessel embolization. The DPD was recycled, the lead wire withdrawn along with the catheter and the surgery completed.

In cases of bilateral carotid artery stenting, surgery was performed as described above and side bracket implantation was utilized. Intraoperative ECG, blood pressure (BP), and pulse oxygen saturation prior to and after balloon predilation or stent implantation was measured and BP was monitored every 2 min. The pre- and intraoperative rate and BP were recorded according to the National Institute of Health Stroke Scale (<https://www.ninds.nih.gov/doctors/NIH_Stroke_Scale.pdf>). Pacemakers in patients with one were monitored.

### Diagnosis and removal of common complications during surgical resection

#### Vasospasm

Vasospasm was diagnosed through angiography. The appearance of rough, jagged, wavy and irregular stenosis of the vascular wall was diagnosed as vascular spasm ([@b18-etm-0-0-3360]). Vasospasm was mostly localized in the vicinity of DPD and stent. At the end of surgery, if the vasospasm of the vascular wall was evident, papaverine hydrochloride (30 mg/20 ml of 0.9% saline) was intravenously administered. After 5 min angiography was carried out to determine whether or not the vasospasm disappeared.

#### Hemodynamic depression

The diagnostic criteria for hemodynamic depression were CAS intraoperative or postoperative symptomatic or non-symptomatic BP decrease (systolic BP \<90 mmHg) and bradycardia (heart beat rate \<60/min) ([@b3-etm-0-0-3360]), regardless of whether booster or heart rate-increasing drugs or pacemaker were used. The pacing rate of preventive use of temporary cardiac pacing in patients was set at 60/min. If intra- and postoperative ECG exhibited an explicit pacing signal, hemodynamic depression was considered to exist. In case of intraoperative hemodynamic depression, atropine sulfate (0.25--0.5 mg) or dopamine hydrochloride (150 mg in 35 ml of 0.9% saline) was administered. Atropine sulfate was administered in a discontinuous manner when no rebound of heart rate was observed.

#### Other miscellaneous complications

Acute stent thrombosis, carotid dissection, plaque prolapse, and distal vascular embolism were also observed. However, because of their extremely low incidence, they were considered for analyses in the present study.

#### Postoperative monitoring

Parameters such as ECG, BP and pulse oxygen saturation were monitored following surgery for ≥24 h. Of these, BP was monitored once every 5--30 mins. The antihypertensive drugs were discontinued for hemodialysis patients. If the heart rate was persistent at \<60/min, hydrochloric acid isopropyl epinephrine (2 mg in 500 ml of 0.9% saline) was administered to increase the heart rate. If systolic BP was \<90 mmHg, dopamine hydrochloride or a combination of dopamine hydrochloride and hydroxylamine was administered. Patient consciousness and physical activity were continuously monitored, and if abnormal, the patients\' state was evaluated through analyses of markers of myocardial injury, ECG, magnetic resonance imaging or CT examinations. The patients were treated with clopidogrel (75 mg/day) and aspirin (100--200 mg/day) for ≥3 months.

#### Postoperative follow-up

The in-patients were monitored in the hospital whereas discharged patients were monitored when they visited the outpatient facility and via telephone enquiries for a period of 30 days post-surgery. The MACCE were considered when the patients experienced TIA, ischemic or hemorrhagic stroke, acute myocardial infarction or mortality during the postoperative monitoring period. Manifestation of stroke observed was stratified into large and minor, large stroke was 4--5 points on the modified Rankin Scale (mRS) ([@b19-etm-0-0-3360]) and included patients not capable of self-care, walking alone or were bedridden, coupled with the presence of aphasia or hemianopsia. Minor stroke was 2--3 points on the mRS in which patients had neurological dysfunction, self-care and absence of hemianopsia and aphasia ([@b20-etm-0-0-3360]).

### Statistical analysis

#### Chi-square test and independent sample t-test

The patients were divided into the MACCE and non-MACCE groups. The mean values of continuous variables were compared using the independent t-test. In cases of categorical variables and ≥5 patients, the Pearson Chi-square test was applied. If the number of patients were \<5, the continuity corrected Chi-square test was used, and for \<1, the Fisher\'s exact test was used. P\<0.05 was considered to indicate a statistically significant difference.

#### Unconditional logistic regression analysis

The variables which showed a significant difference in the Chi-square test and t-test were included in the dual unconditional logistic regression analysis. The variables were evaluated for their effect and influence on MACCE. The odds ratio (OR) and 95% confidence interval (CI) were calculated for categorical variables. P\<0.05 was considered statistically significant.

#### Regression model construction

The regression equation was established following examination of the regression coefficient. The likelihood ratio test or Wald\'s Chi-square test was used for single regression coefficient hypothesis testing and the contribution of the model was determined. The test of goodness of fit of the regression model was determined using Hosmer-Lemeshow. The regression equation was expressed as: log (p) = β0 + β1 × 1 +... βn × n. By calculating the normalized regression coefficients of individual variables, the effect of the risk factors associated with the occurrence of MACCE was analyzed.

#### Establishment of the risk score table

The risk score table was established from the regression model. The score was assigned as a decimal number of the OR value of the risk factor associated with the occurrence of MACCE or 0.5 after the integer. This table was used to calculate the risk score and to draw a forecast probability chart of the risk score. The score table was also used to test the forecast effect by drawing the receiver operating characteristic curve (ROC) and to calculate the area under the curve (AUC). The forecast probability chart was created through MS Excel. ROC, AUC and any other statistical tests were carried out using SPSS 18.0 (SPSS, Inc., Chicago, IL, USA).

Results
=======

### Demographics of patients

Of the 433 patients that underwent CAS, 30 patients were excluded from the present study for various reasons, including carotid and vertebral artery stenosis in multiple sites together with stent implantation (16 patients), surgical operation failure (2 patients), loss of follow-up (12 patients). A total of 403 patients were followed up for 30 days subsequent to surgery.

The patient age range was 45--83 years with an average of 66.73±7.03 years. The patients aged 60--70 years were higher in number (40.20%, [Table I](#tI-etm-0-0-3360){ref-type="table"}). There were 298 males and 105 females. Of the various risk factors, hypertension was found in 66.00% of patients. Approximately 65.76% of patients had symptomatic carotid stenosis, 31.76% had TIA and 34.00% had CI.

### Clinical diagnoses

Of the 403 patients, 21.34% were found to have aortic arch calcification, while 23.33% of patients had calcified plaque and 25.31% had ulcerative plaque. The type II aortic arch was identified in the majority of patients (59.55%). The ipsilateral carotid artery tortuosity was observed in 21.34% of patients with aortic arch calcification. The majority of patients had severe carotid stenosis (68.24%). The carotid bifurcation region was the most frequent site of stenosis (92.06%) and most of the lesions were \<15 mm ([Table I](#tI-etm-0-0-3360){ref-type="table"}).

### Perioperative major adverse cardio- and cerebrovascular events

During the follow-up period, MACCE were observed in 33 (8.19%) patients. Of the 33 patients, 16 patients experienced any of the following MACCE events: stroke, myocardial infarction or mortality, and TIA was experienced by 17 patients. The TIA occurred during the surgery (7 patients) and 1--3 days post-surgery in the hospital ([Table II](#tII-etm-0-0-3360){ref-type="table"}).

### Comparison of baseline and clinical parameters between the MACCE and non-MACCE groups

No significant difference in the average age of patients between the MACCE and non-MACCE groups was identified. However, in the subgroup of ≥70 years, MACCE was observed in 28 (84.85%) of 33 patients, which showed its significant association with the incidence of MACCE (P\<0.001). The remaining clinical parameters significantly associated with the incidence of MACCE included diabetes mellitus, coronary cardiopathy and symptomatic carotid stenosis (P\<0.05, [Table III](#tIII-etm-0-0-3360){ref-type="table"}).

In addition to these results, ulcerative plaque, type III aortic arch, severe stenosis, longer lesion and carotid artery occlusion were significantly associated with the incidence of MACCE (P\<0.05, [Table IV](#tIV-etm-0-0-3360){ref-type="table"}).

### Intraoperative situation between MACCE and non-MACCE groups

The DPD was used in 389 (96.53%) patients but not in 14 patients because of severe tortuosity of the internal carotid artery. A majority (18.18%) of patients in the MACCE group received bilateral carotid stenting in comparison with patients in the non-MACCE group (5.41%). The incidence of intraoperative hemodynamic depression was higher in the MACCE group at 31 (93.94%) of 33 compared to that of the non-MACCE group at 210 of 370 patients (56.76%, [Table V](#tV-etm-0-0-3360){ref-type="table"}).

### Additive effect of risk factors on the incidence of MACCE

The parameters that were significantly associated with the incidence of MACCE were included in the dual non-conditional logistic regression analysis. After removing the confounding factors, only age ≥70 years, ulcerative plaque, severe stenosis, bilateral carotid stenting and hemodynamic depression were found to be independently associated with the incidence of MACCE ([Table VI](#tVI-etm-0-0-3360){ref-type="table"}).

The effect of the aforementioned risk factors on the incidence of MACCE was expressed using the regression equation: Log (MACCE incidence probability) = −8.992 + 1.609 × age ≥70 + 1.064 × ulcerative plaque + 1.245 × severe stenosis + 1.611 × bilateral carotid stenting + 1.757 × hemodynamic depression (χ^2^=163.478, P\<0.001).

These findings showed that, age, ulcerative plaque, severe stenosis, bilateral carotid stenting and hemodynamic depression were significantly associated with the incidence of MACCE. The cumulative probability plot ([Fig. 1](#f1-etm-0-0-3360){ref-type="fig"}) shows that, the scatter is basically diagonal and the Hosmer-Lemeshow goodness-of-fit test indicated the model has good fitting effect.

The standard regression coefficient analysis revealed that, hemodynamic depression was the most prominent risk factor for MACCE followed by bilateral carotid artery, age ≥70 years, severe stenosis and ulcerative plaque ([Table VII](#tVII-etm-0-0-3360){ref-type="table"}).

### Risk score table for MACCE

The risk scores of the risk factors for MACCE were: 5 points for age ≥70 years, 3 points for ulcerative plaques, 3.5 points for severe stenosis, 5 points for bilateral carotid artery stenting and 6 points for hemodynamic depression.

These points were used to calculate each of the 403 patient risk scores and create the forecast probability chart. The risk of incidence of MACCE increased with an increasing risk score ([Fig. 2](#f2-etm-0-0-3360){ref-type="fig"}).

To validate the predictive effect of risk scores, the ROC curve was drawn and AUC was found to be 0.875 (P\<0.001, 95% CI: 0.825--0.925), which indicated that a good forecast effect of this risk score model for MACCE ([Fig. 3](#f3-etm-0-0-3360){ref-type="fig"}).

Discussion
==========

Currently, stroke ranks fourth among all causes of mortality in the United States and is recognized as a leading cause of serious physical and cognitive long-term disability in adults. Almost 800,000 US residents experience an incident or recurrent stroke each year ([@b21-etm-0-0-3360]). In China, the annual incidence of stroke is approximately 2 million, of which 1.5 million patients succumb, while the majority of survivors (75%) become disabled. Additionally, the 5-year survival rate is 41% ([@b22-etm-0-0-3360]). Ischemic stroke was involved in 75--85% of all stroke incidences for which carotid atherosclerotic stenosis is one of the major contributing factors. After decades of clinical practices, CEA was considered the gold standard for the treatment of carotid atherosclerotic stenosis. Continuous advancements in the field of neuroimaging and vascular operation technology in the last decade have led to CAS potentially becoming an alternative treatment to CEA for carotid atherosclerotic stenosis ([@b23-etm-0-0-3360]). However, there is a lack of sufficient evidence to verify this hypothesis as the MACCE were not less in CAS than CEA during the perioperative period ([@b24-etm-0-0-3360]). Consequently, CAS is often recommended as an alternative treatment method to CEA only in high-risk patients ([@b25-etm-0-0-3360]).

Previous findings have suggested that, the incidence of ipsilateral stroke was extremely low in patients who underwent CAS in comparison with CEA ([@b26-etm-0-0-3360]). Therefore, we carried out risk assessment for MACCE during the perioperative period of CAS that would reduce MACCE during the perioperative period of CAS by providing intervention to complications that were significantly associated with MACCE. However, investigations pertaining to the risk of stroke, myocardial infarction and death during the CAS perioperative period are scarce ([@b2-etm-0-0-3360]).

In the present study, MACCE were observed in 8.19% of patients who underwent CAS, of which 3.97% experienced stroke, myocardial infarction or mortality. This incidence rate was similar to that reported worldwide ([@b27-etm-0-0-3360]). Although the univariate analysis revealed 11 risk factors (age, diabetes, coronary heart disease, symptomatic carotid artery stenosis, ulcerative plaque, type III aortic arch, severe carotid artery stenosis, longer lesion, carotid artery occlusion, bilateral carotid artery stenting and hypertension) associated with the incidence of MACCE, the multivariate analysis revealed the association of only five risk factors (age ≥70, ulcerative plaque, bilateral carotid artery stenting, severe stenosis and hypertension) for the incidence of MACCE and a potent risk model.

In a previous study, age was found to be an independent risk factor associated with postoperative complications ([@b28-etm-0-0-3360]), because elderly patients are more prone to artery tortuosity and vascular calcification ([@b29-etm-0-0-3360]). The instability of ulcerative plaque increases the risk of TIA and ischemic stroke caused by thromboembolism during or after surgical resection ([@b30-etm-0-0-3360]). There is a high risk of thromboembolism during endovascular surgery where the catheter, guidewire and DPD are used to reach the lesion.

The present study also showed that bilateral carotid artery stenting is a risk factor of MACCE in CAS patients. The operation time in the blood vessels was prolonged subsequent to bilateral stent release, thus, cerebral perfusion increased, which ultimately leads to increased risk of thrombosis or cerebral hemorrhage. Severe carotid stenosis was also found to be a risk factor for MACCE. Due to preoperative cerebral hypoperfusion and ascertaining adverse collateral circulation following the discharge of bracket, severe carotid stenosis is more prone to cerebral hyperperfusion leading to hemorrhagic stroke.

Previous findings have shown that, hypertension is linked with other conditions such as coronary disease ([@b31-etm-0-0-3360]), type of aortic arch ([@b32-etm-0-0-3360]), carotid artery tortuosity, calcification ([@b33-etm-0-0-3360]), balloon predilation, bilateral stent implantation and other factors ([@b34-etm-0-0-3360]). In the present study, the interaction between these factors constitutes hypertension as a potent risk factor. A possible reason for observing CI and myocardial infarction in the current study was the decreased BP and bradycardia caused by the intra- and postoperative slowdown of clearance of emboli and plaque debris that weaken the stenosis vascular tortuosity of collateral circulation, thereby increasing the risk of cerebral ischemia and myocardial infarction.

The results of the present study established a risk score model for MACCE that may be used to quantify the risk for MACCE in CAS patients through which effective measures can be taken to reduce the incidence of MACCE in CAS patients during their perioperative period.

![Standard residual cumulative probability normal P-P diagram..](etm-12-02-1039-g00){#f1-etm-0-0-3360}

![Forecast probability of the risk score.](etm-12-02-1039-g01){#f2-etm-0-0-3360}

![Forecast effect of the risk score. ROC, receiver operating characteristic.](etm-12-02-1039-g02){#f3-etm-0-0-3360}

###### 

Baseline characteristics and imaging features of CAS patients.

  Characteristics                              Numerical value (n=403)
  -------------------------------------------- -------------------------
  Baseline characteristics                     
    Demographic data                           
      Age (years old, mean ± SD)               66.73±7.03
      \~80 years old, no. (%)                  5 (1.24)
      70--80 years old, no. (%)                150 (37.22)
      60--70 years old, no. (%)                162 (40.20)
      \~60 years old, no. (%)                  86 (21.34)
      Male, no. (%)                            298 (73.95)
      Female, (%)                              105 (26.05)
    Vascular risk factors                      
      Hypertension, no. (%)                    266 (66.00)
      Diabetes mellitus, no. (%)               108 (26.80)
      Hypercholesterolemia, no. (%)            88 (21.84)
      Smoking, no. (%)                         159 (39.45)
    Coronary cardiopathy, no. (%)              102 (25.31)
    Symptomatic carotid stenosis               265 (65.76)
      TIA, no. (%)                             128 (31.76)
      Cerebral infarction, no. (%)             137 (34.00)
  Imaging features                             
    Aortic arch calcification, no. (%)         86 (21.34)
    Calcified plaque, no. (%)                  94 (23.33)
    Ulcer type plaque, no. (%)                 102 (25.31)
    Aortic arch typing                         
      Aortic arch type I, no. (%)              114 (28.29)
      Aortic arch type II, no. (%)             240 (59.55)
      Aortic arch type III, no. (%)            49 (12.16)
    Carotid artery tortuosity, no. (%)         217 (53.85)
    Carotid artery stenosis                    
      ≥70%, no. (%)                            275 (68.24)
      \<70%, no. (%)                           128 (31.76)
    Lesion length                              
      ≥15 mm, no. (%)                              121 (30.02)
      \<15 mm, no. (%)                         282 (69.98)
    Contralateral carotid occlusion, no. (%)   16 (3.97)
    Lesions carotid bifurcation, no. (%)       371 (92.06)

SD, standard deviation; TIA, transient ischemic attack.

###### 

Post-operative adverse cardiovascular and cerebrovascular events in CAS patients.

  Cerebral vascular event                                 Values, n=403
  ------------------------------------------------------- ---------------
  TIA, no. (%)                                            17 (4.22)
  Stroke of any cause, no. (%)                            15 (3.72)
    Large stroke (operative side), no. (%)                  4 (0.99)
    Large stroke (non-operative side), no. (%)              1 (0.25)
    Minor stroke (operation side), no. (%)                  9 (2.23)
    Minor stroke (non-operation side), no. (%)              1 (0.25)
  Myocardial infarction, no. (%)                            1 (0.25)
  Death of any cause, no. (%)                               3 (0.74)
  Stroke, myocardial infarction and death, no. (%)        16 (3.97)
  TIA, stroke, myocardial infarction and death, no. (%)   33 (8.19)

CAS, carotid angioplasty with stenting; TIA, transient ischemic attack.

###### 

Baseline characteristics of the MACCE and non-MACCE groups.

  Characteristics                                                    Non-MACCE group (n=370)   MACCE group (n=33)   Statistic     P-value
  ------------------------------------------------------------------ ------------------------- -------------------- ------------- -------------------------------------------------------
  Age (years old, mean ± SD)                                         69.00±6.85                66.53±7.02           t=1.939       0.053
    ≥70 years old, no. (%)                                           126 (34.05)               28 (84.85)           χ^2^=33.108   \<0.001^[a](#tfn3-etm-0-0-3360){ref-type="table-fn"}^
    \<70 years old, no. (%)                                          244 (65.95)               5 (15.15)                          
  Gender                                                                                                                          
    Male, no. (%)                                                    277 (74.86)               21 (63.64)           χ^2^=1.983    0.159
    Female, no. (%)                                                    93 (25.14)              12 (36.36)                         
  Hypertension, no. (%)                                              241 (65.14)               25 (75.76)           χ^2^=1.524    0.217
  Diabetes mellitus, no. (%)                                           93 (25.14)              15 (45.45)           χ^2^=6.377    0.012^[a](#tfn3-etm-0-0-3360){ref-type="table-fn"}^
  Smoking, no. (%)                                                   145 (39.19)               14 (42.42)           χ^2^=0.133    0.716
  Hypercholesterolemia, no. (%)                                        78 (21.08)              10 (30.30)           χ^2^=1.510    0.219
  Coronary cardiopathy, no. (%)                                        84 (22.70)              18 (54.55)           χ^2^=16.251   \<0.001^[a](#tfn3-etm-0-0-3360){ref-type="table-fn"}^
  Symptomatic carotid stenosis (TIA, cerebral infarction), no. (%)   237 (64.05)               28 (84.85)           χ^2^=5.818    0.016^[a](#tfn3-etm-0-0-3360){ref-type="table-fn"}^

P\<0.05. SD, standard deviation; TIA, transient ischemic attack; MACCE, major adverse cardiac and cerebrovascular events.

###### 

Comparison of imaging features of the MACCE and non-MACCE groups.

  Characteristics                            Non-MACCE group (n=370)   MACCE group (n=33)   Statistic     P-value
  ------------------------------------------ ------------------------- -------------------- ------------- -------------------------------------------------------
  Aortic arch calcification, no. (%)         78 (21.08)                8 (24.24)            χ^2^=0.180    0.671
  Patch properties                                                                          χ^2^=2.013    0.156
    Calcified plaque, no. (%)                83 (22.43)                11 (33.33)                         
    Non-calcified plaque, no. (%)            287 (77.57)               22 (66.67)                         
  Ulcer type plaque, no. (%)                 83 (22.43)                19 (57.58)           χ^2^=19.794   \<0.001^[a](#tfn4-etm-0-0-3360){ref-type="table-fn"}^
  Aortic arch typing                                                                        χ^2^=7.700    0.021^[a](#tfn4-etm-0-0-3360){ref-type="table-fn"}^
    I, no. (%)                               106 (28.65)               8 (24.24)                          
    II, no. (%)                              224 (60.54)               16 (48.49)                         
    III, no. (%)                             40 (10.81)                9 (27.27)                          
  Carotid artery tortuosity                  194 (52.43)               21 (63.64)           χ^2^=1.528    0.216
  Carotid artery stenosis                                                                   χ^2^=4.575    0.032^[a](#tfn4-etm-0-0-3360){ref-type="table-fn"}^
    ≥70%, no. (%)                            247 (66.76)               28 (84.85)                         
    \<70%, no. (%)                           123 (33.24)               5 (15.15)                          
  Lesion length                                                                             χ^2^=5.830    0.016^[a](#tfn4-etm-0-0-3360){ref-type="table-fn"}^
    ≥15 mm, no. (%)                          105 (28.38)               16 (48.48)                         
    \<15 mm, no. (%)                         265 (71.62)               17 (51.52)                         
  Contralateral carotid occlusion, no. (%)   12 (3.24)                 4 (12.12)            χ^2^=4.151    0.042^[a](#tfn4-etm-0-0-3360){ref-type="table-fn"}^
  Lesions cross bifurcation, no. (%)         343 (92.70)               28 (84.85)           χ^2^=2.557    0.110

P\<0.05. MACCE, major adverse cardiac and cerebrovascular events.

###### 

Comparison of intraoperative situation of the MACCE and non-MACCE groups.

  Characteristics                         Non-MACCE group (n=370)   MACCE group (n=33)   Statistic     P-value
  --------------------------------------- ------------------------- -------------------- ------------- -------------------------------------------------------
  DPD use, no. (%)                        359 (97.03)               30 (90.91)           χ^2^=1.803    0.179
  DPD type, no. (%)                                                                                    
    Angioguard                            164 (44.33)               9 (27.27)            χ^2^=2.757    0.097
    Spider                                195 (52.70)               21 (63.64)                         
  Bracket type, no. (%)                                                                  χ^2^=3.350    0.187
    Precise                               212 (57.30)               14 (42.42)                         
    Protégé                               143 (38.65)               16 (48.48)                         
    Smart Control                         15 (4.05)                 3 (9.09)                           
  Balloon pre expansion, no. (%)          47 (12.70)                8 (24.24)            χ^2^=3.423    0.064
  Bilateral carotid stenting, no. (%)     20 (5.41)                 6 (18.18)            χ^2^=8.195    0.004^[a](#tfn5-etm-0-0-3360){ref-type="table-fn"}^
  Intravascular operation time, no. (%)                                                  χ^2^=0.988    0.320
    \<30 min                              276 (74.59)               22 (66.67)                         
    ≥30 min                               94 (25.41)                11 (33.33)                         
  Vasospasm, no. (%)                      52 (14.05)                6 (18.18)            χ^2^=0.419    0.517
  Hemodynamic depression, no. (%)         210 (56.76)               31 (93.94)           χ^2^=15.912   \<0.001^[a](#tfn5-etm-0-0-3360){ref-type="table-fn"}^

P\<0.05. MACCE, major adverse cardiac and cerebrovascular events; DPD, distal protection device.

###### 

Association of risk factors with MACCE.

  Risk factor                  B       SE      Wals    OR (95% CI)             P-value
  ---------------------------- ------- ------- ------- ----------------------- ---------
  Age (≥70) years              1.609   0.571   7.949   4.997 (1.633--15.290)   0.005
  Ulcerative plaque            1.064   0.444   5.740   2.899 (1.214--6.924)    0.017
  Severe stenosis              1.245   0.568   4.807   3.472 (1.141--10.566)   0.028
  Bilateral carotid stenting   1.611   0.628   6.575   5.007 (1.462--17.151)   0.010
  HD                           1.757   0.792   4.915   5.792 (1.226--27.369)   0.027

MACCE, major adverse cardiac and cerebrovascular events; OR, odds ratio; CI, confidence interval; B, regression coefficient; SE, standard error; HD, haemodynamic depression.

###### 

MACCE in perioperative period of CAS.

  Characteristics              B *j*   S *j*   Standardized regression coefficient
  ---------------------------- ------- ------- -------------------------------------
  Age (≥70) years              1.609   0.571   0.502
  Ulcerative plaque            1.064   0.444   0.258
  Severe stenosis              1.245   0.568   0.386
  Bilateral carotid stenting   1.611   0.628   0.553
  HD                           1.757   0.792   0.760

MACCE, major adverse cardiac and cerebrovascular events; HD, haemodynamic depression.
