086729ce-50ea-45ec-b89e-219ca792ac06
prompter
What are the strategies to overcome diffusion limitation in growing cells in suspension?
ed6c81af-93cd-4a41-a2ce-13fd89c4daad
086729ce-50ea-45ec-b89e-219ca792ac06
assistant
Diffusion limitation can be overcome in growing cells in suspension by implementing the following strategies:

1) Increase culture volume: By increasing the volume of the culture, the concentration of nutrients and oxygen can be kept at adequate levels, reducing the impact of diffusion limitation.

2) Stirring or agitation: Constant stirring or agitation can help to distribute nutrients and oxygen evenly throughout the culture, reducing the impact of diffusion limitation.

3) Oxygen-permeable membrane: By using an oxygen-permeable membrane, the oxygen levels can be regulated, helping to overcome diffusion limitation.

4) Microcarriers: Microcarriers can be used to support the growth of cells in suspension, providing a large surface area for cells to attach and access nutrients, which can help to overcome diffusion limitation.

5) Sparging: The process of sparging, where air or oxygen is bubbled through the culture, can help to increase the oxygen levels and reduce the impact of diffusion limitation.

6) Nutrient supplementation: Supplementing the culture with additional nutrients can help to maintain optimal nutrient levels, reducing the impact of diffusion limitation.
2c89840a-46b1-458f-b244-d84d042719f8
ed6c81af-93cd-4a41-a2ce-13fd89c4daad
prompter
Ah, and just to be sure, what mediums are best suited for culture development? I hear agar is common for petri dish growth, but what of suspension?