Therefore, screening for new antibiotics become a critical need Objectives: Synthesis of some 1, 4-naphthoquinone derivatives by replacing the hydrogen atom at 2-position by other chemical groups in order to improve the antibacterial activity. Methods: 1, 4-naphthoquinone structure has been modified by several reactions such as: nitrosonation followed by reduction, diazonisation, acylation and amino substitution.