Background: To use a new substance (2-methoxy-1, 4-naphtoquinone) as an antifungal agent, this substance has been determined the antifungal activity in vitro and in vivo. Objective: To determine the antifungal activity in vitro and ex vivo of 2-methoxy-1, 4 naphthoquinone, a principle compound of balsam extract, extracted from Impatiens balsamina L Balsaminaceae which was synthesized against Candida spp compare with balsam extract and some other antifungal agents.