The percentage of bacteria identified after performing anaerobic culture, Realtime polymeraase chain reaction technique: Aggregatibacter actinomycetemcomitans 11, 3%, Porphymonas gingivalis 0%, F nucleatum 0%, Tannerella forsythia 5, 7%, Parvimonas micra 11, 4%, Veillonella parvula 45, 7%, Campylobacter showae 5, 7%, Prevotella intermedia 22, 9%, Trenponema dencota 11, 4%.