Subject and method: Sixteen adult Fasciola spp. were collected from buffalo (5 isolates), bovine (6 isolates), sheep (2 isolates), goats (2 isolates), and human (1 isolate) from slaughterhouse in Hanoi city (4 isolates), Vinh Phuc (1 isolate), Nghe An (2 isolates), Quang Nam (1 isolate), Ninh Thuan (4 isolates), Tay Ninh province (1 isolate), Can Tho city (1 isolate), and Dong Thap province (2 isolates), Vietnam, from 2009 to 2016.