The mean time to perform gelcard technique was 30, 1 ± 2, 1 minutes, significantly shorter than the tube technique with 60, 4 ± 12, 3 minutes (p < 0, 05); the mean storage time for retrieval of gelcard technique was 5 ± 0, 56 days, which of tube technique was just maximally 1 day (p < 0, 05).