The 5-methoxybenzimidazole-2-thione acylation reaction created the products which substituted in N positions. While the HEPG2 cancer cell strain was inhibited by 2/9 tested compounds, the MDA cancer cell strain was inhibited by 5/9 tested substances, the RD cancer cell strain was inhibited by 7/9 test substances, there were three experimental substances that have influenced the tested normal cell strain.