Results: charantin content of bitter melon in Thua Thien Hue extracted with a solvent mixture of MeOH - CHCl3 (1: 1 v / v) was found to be 0.204%; antioxidant activity of FRAP content of fresh bitter melon extracted with solvent methanol was found to be 972.16 μmol Fe2+ / L; the samples exhibited the antibacterial activity for four strains of bacterial pathogens with antimicrobial diameters from 14 - 17 mm.