Wet mounts Wet mounts of unstained samples can be used to detect the following via darkfield microscopy: Fungi Parasites (including helminth eggs and larvae) Vaginal clue cells (present in bacterial vaginosis) Motile organisms (eg, Trichomonas) Treponema spirochetes (present in syphilis) Visibility of fungi can be increased by applying 10% potassium hydroxide (KOH) to dissolve surrounding tissues and nonfungal organisms.