This research used 4 techniques (Inversion PCR, Multiplex PCR, PCR and Gene Sequencing) to determine mutations on 103 Hemophilia A patients. The result showed that 89, 3% (92/103) of patients have F8 gene mutation included: 38.1% of patients (35/92) with intron 22 inversion mutation, 23.9% of patients (22/92) with missense mutation, 9.8% of patients (9/92) with small deletion mutation, 9.8% of patients (9/92) with nonsense mutation, 9.8% of patients (9/92) with insertion deletion, 4.4% of patients (4/92) with splicing site mutation and 4.4% of patients (4/92) with large deletion mutation.