Background: There is a gap in health care service supply and health outcome in urban and sub-urban areas. Objectives: To determine and analyze the gap in health status and accessibility with health care service between different regional stratification in Ho Chi Minh City, Vietnam, in 2009.Methodology: The matrix of health equity in Ho Chi Minh City base on selected indicators of Urban HEART and give the response phases. Result: There are differences between urban and sub-urban areas in Ho Chi Minh City in some diseases which need to be interested.