Several surgical techniques were applied to deal with anal fistula, but these operations somehow damaged the anal sphincters. Objective: This study was aimed to evaluate the result of the ligation of intersphincteric fistula tract for anal fistula. Patients and Methods: The study was the study of consecutive cases in the University Medical Center at Ho Chi Minh city from Jan 2008 to May 2010.Results: There were 22 patients (18 male and 4 female) with anal fistula underwent this operation.