We determined some main characteristics of real-time PCR including: (1) The standard curves for HBV and HCV quantitation have a correlation coefficient of 0, 989 and 0, 996 respectively, (2) The intra-assay variability coefficient is 0, 2 - 0, 5 for HBV and 0, 76 - 2, 05 for HCV, (3) The inter-assay variability coefficient is 2, 5 - 10, 9 and 2, 4 - 8, 81 for HBV and HCV respectively (4) The detection limit of the two reactions is 101 copies/ml.