Objective: Evaluate using Virotrol I for TTI's testing on donation at Immunology Department. Subjects and Methods: Using Virotrol I in every machine for Screening test (HBV, HCV, HIV, HTLV) with Elisa techniques and Chemiluminescence, from March 1, 2011 to August 15, 2011.Results: Using Virotrol I for 4 TTI's testing at Blood Transfusion Hematology Hospital on two machine models shown that: total of sample was Virotrol I made on the Evolis with 1477 times and Architect machine were 492 times.