Therefore, the survey of microorganism's pollution in the air surgery room, recovery room is necessary to ensure the health of patients and communities. Objectives: Determination of pollution levels about total aerobic bacteria, mold spores total, soluble blood group A Streptococcus, Staphylococcus aureus, Pseudomonas aeruginosa in the operating room, recovery room in 13 hospitals in Ho Chi Minh City. Method: Descriptive cross - sectional design, 13 hospitals located in Ho Chi Minh City including hospitals under the Ministry of Health, the hospitals under the Department of Health and private hospitals.