Conclusion: The method can be potentially applied for analysis of oriental medicines for the treatment of musculoskeletal disease containing betamethasone, ketorprofen, dexamethasone acetate and indomethacin Key words: betamethasone, ketorprofen, dexamethasone acetate, indomethacin; high performance liquid chromatography (HPLC); herbal medicines for the treatment of musculoskeletal disease.