This research has identified the formula of AO NIN premix (mixture of vitamins C, D, E, β-carotene), identified manufacturing recipe: 16% ashitaba sap powder, 42% AO NIN premix, 39% anhydrous lactose, 2% magnesium stearate, 1% talc and mix optimization method: mixing ashitaba sap powder with lactose anhydrous ashitaba first, then mixed with Premix and slippery.