Structures of them were determined by spectral data of nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS). Results & Conclusion: Structures of 2 polyoxygenated steroids were identified: (1) ergosta-3β, 5α, 6β, 11α-tetraol (sarcoaldesterol B) and (2) ergosta-1β, 3β, 5α, 6β-tetraol.