Purpose: We study the prevalence of HPV and subtype in squamous cell carcinoma of the penis and the relationship between HPV infection and epidemiologic, clinical, phthological factors. Material and methods: Investigations were carried out on 48 penile cancer patients treated at oncology hospital of HCM city, between 08/2009 and 03/2010. The technique of PCR is used to detect the presence of HPV on penile spiecemen.