Diagnosis of Hemolytic Anemia Peripheral smear and reticulocyte count Serum bilirubin, lactic dehydrogenase (LDH), haptoglobin, and alanine aminotransferase (ALT) Antiglobulin (Coombs) test and / or hemoglobinopathy screen Hemolysis is suspected in patients with anemia and reticulocytosis.