Objectives: Study of a simultaneous quantititative method for DIOSFLON film-coated tablets containing diosmine 450 mg and hesperidine 50 mg. Method: Extraction of disomine and hesperidine by using dimethyl sulfoxyd (DMSO) prior to application to HPLC Results: The validated HPLC procedure allowed to analyze quantitatively hesperidine và diosmine in the product.