Laboratory diagnosis is usually unnecessary but can often be made by Culturing the virus Detecting viral RNA using RT-PCR Less commonly, demonstrating seroconversion Enteroviruses can be detected using RT-PCR testing on a sample from the involved site (eg, throat or nasopharynx, blood, cerebrospinal fluid) or from stool, in which the organism is often present even when the clinically involved site is elsewhere; identifying the causative organism is important mainly in cases of aseptic meningitis.