Method: Using electrochemical biosensor based on DNA immobilization as probe for the rapid detection of Herpes Simplex Virus (HSV) genome from standard samples and its PCR productResults: Our DNA based electrochemical biosensor shown a high sensitivity, the detection limit is 0.5nM of sample and the stable response time of the biosensor is approximately 5 min. Conclusion: Our DNA based electrochemical biosensor can be developed and applied for rapid detection of one or simultaneous multi-virus genomes in Vietnam.