The rate of negative Rh (D) is highest (14.1%) in AB group, and is lowest in O group (0.29%); A and B groups have the same rate of negative Rh (D) (0.62%); p < 0.05.Conclusion: Research on the 3, 105 volunteer blood donors shows that: the rate of ABO blood group system follows the rule O> B> A> AB.