The DIM extract showed good anti - inflammatory activity, inhibition of nitric oxide (NO) production with IC50 value of 16.1 g / mL; good antioxidant activity by DPPH assay with SC50 value of 20.03 µg / mL; and in vitro relatively good cytotoxic activity against two human cancer cell lines: liver cancer HepG2 cells and carcinoma KB cells with IC50 values of 17.07 µg / mL and 20.56 µg / mL, respectively.