DNA was extracted from buccal swab samples or hair-root samples and then using multiplex primers kit of Beckman Coulter special for12 STR loci (TH001, D18S51, AMOELOGEN, D13S1357, D7S820, D16S539, PENTA E, D3S1358, D8S1179, TPOX, CSF1PO, PENTA D) to amplified by PCR.