Purpose: Research on knowledge, attitude and positive behaviors for eye donation is carried out in Ho Chi Minh City in order to provide a scientific foundations and pratical applications to help defining a suitable strategy to motivate eye donation behaviors to create the supply for the Eye Bank. Results: According to our research, people who are in the age of 18 - 34, being pupils, students, living in rural area, single, having income less than 1 million VND per month, and especially having blind relatives normally have acceptance attitute towards eye donation.