The identification of HBV genotypes are more concerned as it is HBV genotypes relating to clinical manifestation, activity of liver disease as well as treatment responses of Hepatitis B Additionally, the discovery of resistance to medicines in HBV treatment plays a very important role from which we can have an appropriate and effective choice in Hepatitis B treatment. Objective and methods: We have applied the Sequencing by OpenGene system with Trugene HBV Genotyping kit (Bayer) to identify Hepatitis B Virus Genotypes and Lamivudine - resistant mutation.