Of which, there are 15 hydrophilic mutations that increase the virulence and infectivity of the virus with the highest appearing probability 48.40%, 6 hydrophobic mutations that preserve the hydrophobicity of ORF8 have the appearing probability 19.35%, 4 hydrophibic neutral mutations that do not change the functions of ORF8 have appearing probability 12.90% and 6 hydrophilic neutral mutations that do not change the functions of ORF8 have appearing probability 19.35%.