However, high cost, need for qualified personnel and inconvenience of collecting clean-voided specimens are the among the main drawbacks. Objectives: identify the sensitivity, specificity, positive predictive value and negative predictive value of urinalysis in diagnosting asymptomatic bacteruria in pregnant women at first trimester. Methods: In this study, we compared urinalysis with standard urine culture for 281 pregnant women at SaiGon International OB & GYN Hospital between January 2010 and June 2010.Results: Of 281 urine specimens evaluated, 21 cases (7.5%) were determined to have ≥ 105 CFU / ml by the culture method.