Results: The formulation with 5% (w / w) of ACV, 4.5% (w / w) of A vera, 5% (w / w) of glycerin and 2% (w / w) of HPMC K100M had the physico-chemical properties suitable for topical administration and the best permeability enhancement as compared to other formulations with different concentrations of A vera, and a commercial cream formulation containing 5% (w / w) of ACV.