Objectives: This study was conducted to describe the situation of sex and substance user (alcohol, metamphetamine, popper) on MSM (men who have sex with men) and TG (transgender woman) in 4 southern province, 2019 Method: A cross-sectional study was used to anayzed data gathered from a group of 1000 MSM and TG, who selected through the support of CBOs (Community Based Organizations) in the study area.