The process improvement points in the preparation of autologous stem cells from adipose tissue for the treatment of chronic obstructive pulmonary disease include: the location of fat collection which is the subcutaneous fat layer around the belly button; the minimum amount of fat collected is 50 ml; preserving stem cells with a dedicated storage bag instead of cryo tubes for faster defrosting after storage, ensuring sterility and reducing the time from thawing to stem cell infusion to reduce the number of dead cells after defrosting.