Besides, a good repeatability and intermediate precisions (RSD < 2%), a reliable accuracy (recovery efficiency between 99.4 and 101.5%, RSD < 1.73%) as well as the obtained limits of quantification in values of 2.55 content of two markers - pinoresinol 4-O-beta-D-glucopyranoside and vladinol F in Pandanus tonkinensis fruits collected from different locations (Thanh Hoa, Hoa Binh, Thai Nguyen) had been determined falling in the range of 0.0250 - 0.0435 mg / g and 0.0243 - 0.0371 mg / g, respectively.