Recently, some Bacillus species were demonstrated to have carotenoids, a natural antioxidant which is thought to be able to prevent some kinds ofcancer. Objectives: To investigate alternative media derived from low cost materials for four carotenogenic Bacillus to replace Tryptic Soy Broth (TSB). Methods: Culture condition like pH, temperature and NaCl concentration for growing carotenogenic Bacillus strains including AT22 (Bacillus licheniformis), AT14 (Bacillus infantis), HC28 (Bacillus vietnamensis), DD1.1 (Bacillus marisflavi) were investigated.