Subjectives and Medthod: 27 consecutive patients with macular hole of stage 2, 3, 4 with different etiologies were recruited for vitrectomy and ILM peel in Retinal Department of VNIO during the period of 1/2015 to 9/2015. ILM peeling was done with the mixture of trypan blue 0.08% and glucose 10% 1: 1 assisted.