β - Thalassaemia is caused by a combination of 2 out of 12 mutations in the β-globin gene including Cd17, Cd41/42, - 28, Cd71/72, IVS1.1, IVS2.654, Cd95, Cd43G, - 29, Cd8/9, - 90 and Cd26 (HbE). In β-thalassemia mutants, Cd17 accounted for 38.43%, Cd 41.42 accounted for 36.86%, - 28 and Cd 71.72 accounted for 6.86% and 6.11%, respectively.