Materials and Methods: Sixty extracted human lower premolar teeth were selected and randomly assigned to six groups (n = 10), according to the root canal sealer and technique used for obturation: (A) Single-cone technique with Cortisomol; (B) Single-cone technique with AH Plus; (C) Single-cone technique with Apexit Plus; (D) Warm vertical condensation technique with Cortisomol; (E) Warm vertical condensation technique with AH Plus; (F) Warm vertical condensation technique with Apexit Plus.