The Antibiotics Sensitivity Test (AST) was performed for detected species. Results: Sixty five of the 214 isolated (30.4%) were ESBL producers; ESBL were mainly produced by E coli (27 strains), K pneumoniae (15 strains) and P aeruginosa (12 strains); The prevalence of ESBL producing bacteria responsible for nosocomial infection was 64.6% (42/65 strains); A high percentage of pus (44.61%), urine (23.07%), blood and sputum (10.77%) isolates produced ESBL; The department with high frequency of ESBL producing bacteria: the surgical departments (49.22%) and Intensive Care Unit (23.07%); The frequency of antibiotic-resistant ESBL (+) bacteria was higer than ESBL (-) bacteria.