Diagnosis of Cytomegalovirus Detection of CMV antigen or DNA Urine culture in infants Often biopsy in immunocompromised patients Serologic testing CMV infection is suspected in Healthy people with mononucleosis-like syndromes Immunocompromised patients with gastrointestinal, CNS, or retinal symptoms Neonates with systemic disease CMV mononucleosis can be differentiated from infectious (EBV) mononucleosis by the usual lack of pharyngitis, a negative heterophile antibody test, and through CMV serologic testing.