The synthesized chiral compounds was standardized on the criteria such as appearance; identification by melting point, infrared absorption spectrophotometry, specific optical rotation and high performance liquid chromatography method; enantiomeric impurities; related substances; organic volatile impurities; moisture; enantiomeric purity. Results: The two draft specifications of the synthesized chiral cis-N-heptyl phthalazinone and cis-N-benzyl phthalazinone derivatives were established Conclusion: The draft specifications can be applied to control the synthesized chiral cis-N-heptyl-phthalazinone and cis-N-benzyl-phthalazinone derivatives to help optimize the synthesis, and standardize the new purity bulk drug.