Result: 68 patients were treated with laparoscopic cholecystectomy using fluorescein ICG, the age mean of the patients were 55.4 ± 16.2 years, and the male / female ratio was 1.52. Chronic cholecystitis caused by stones accounted for the majority (51.47%); using ICG fluorescence detected 7.35% of cases with anatomical changes of the extrahepatic biliary tract, clearly identifying the anatomy of the common bile duct and the cystic duct was 100% and 92.65%, the average of surgical time was 42.8 ± 14.6 minutes.