Determination protein content by Lowry method and amino acids by GC-faast; HPLC by Shimadzu LC20 AD, finding phenyl isothiocyanate (PITC), columns Lichroson b C18, detecter: 254 nm. Results: Define protein and amino acids in leaves Moringa oleifera by chemical reaction is positive, quantitative amino acids by TLC and finding by reagent Ninhydrin 0.2% in aceton, solvent phenol: NH3 (3: 1) and n-butanol: acid acetic: H2O (4: 1: 1) was separation good amino acids.