Results and discussion: The chromatography condition of simultaneous quantitation of L-Arginine aspartate and L-Lysine hydrochloride: Eclipse Plus C18 column (250 × 4.6 mm, 5 mm), UV detection at 338 nm Mobile phase contains acetonitrile and phosphate buffer pH 7.8, gradient, flow rate was adjusted to 1.0 ml / min and column oven temperature was kept at 40 oC, injection volume was 20ml.