The amount of scopolamine hydrobromide permeated through rat skins per time was determined by validated HPLC method. Results: Among experimented enhancers, the hydrophylic enhancers (Dimethyl sulfoxide (DMSO), methyl butanol, octanol, hexanol, decanol, transcutol P) had a tendency to increase the permeation of scopolamine hyrobromide more than the lipophylic ones (mineral oil, acid oleic, isopropyl myristat), in which DMSO generated saturated solution with the highest amount of scopolamine hydrobromide (252233.93 µg / ml) with the most target flux of scopolamine for rat skin is (28.17µg / cm2 / hour) in the most suitable lag time (5.76 hours).