Method: The cross‐sectional descriptive study and empirical analysis on 176 strains of Escherichia coli and 170 strains of Klebsiella pneumoniae was isolated at Bac Lieu General Hospital from July 2016 to March 2017. Results: The rate of ESBL-producing E coli and K pneumoniae was 61.36% and 24.71%, carbapenemase-producing E coli and K pneumoniae was 6.82% and 4.12%.