Results: A Standardized HPLC method was developed for quantitative determination of sparfloxacin in serum of rabbits with following chromatographic conditions: Water X Tera MS C18 (3x50 mm; 5µm); eluent - a mixture of acetonitrile and 0, 025M phosphate buffer pH 3, 0 (20: 80); flow rate of 1 ml / min; UV-detector at 297 nm.