HLA-A * 11, A * 02, A * 24, B * 15, B * 46, B * 07, Cw * 07, Cw * 08, Cw * 01, DRB1 * 12, DRB1 * 09, DRB3 * 03, DRB3 * 02, DRB4 * 01, DRB5 * 01, DQB1 * 03 and DQB1 * 05 were predominant in this study. Conclusion: Our study showed the application of the PCR-SSP for identifying of the HLA alleles was responded to the hematopoietic stem cell transplatation at the Blood Transfusion Hematology Hospital - Ho Chi Minh City.