In this research, the BALSINO ointment was further studied for the following aspects: the preformulation - dealing with the improvement of the extraction processes of Calophyllum inophyllum oil and Curcuma longa oil, the specifications of the raw material oils, the optimal combination ratio of Calophyllum inophyllum oil with Tersol (a specific fraction produced from Melaleuca leucadendron essential oil); the drug development - involving the computer-aided formulation optimization the standard of the finished product and the product stability; the in vitro and in vivo pharmacological effects - including the antimicrobial activities, the cicatrizing and anti-inflammatory effects as well as the irritatant and toxic properties; the clinical trials - starting with phase I All results were positively evaluated.