Methods: Evaluate the separation of 20 amino acids including: L-alanine (Ala), L-arginine (Arg), L-aspartic acid (Asp), L-cystine (Cyt), L-glutamicacid (Glu), L-glycine (Gly) L-Histidine (His), L-isoleucine (Ile), L-leucine (Leu), L-lysine (Lys), L-methionine (Met), L-phenylalanine (Phe), L-proline (Pro), L-serine (Ser), L-threonine (Thr), L-tyrosine (Tyr), L-valine (Val), L-Cystein (Cys), L-Tryptophan (Try), Taurin (Tau) using Primesep 100 colunm combine with high performance liquid chromatography (HPLC) and MS detector. After that, investigate sample processing method to eliminate interference subtances and validate the establishing method.