Serologic testing for anticoccidioidal antibodies includes Enzyme immunoassay, which is very sensitive and is commonly used to diagnose coccidioidomycosis Immunodiffusion kit (to detect IgM or IgG antibodies) Complement fixation (to detect IgG antibodies) Titers ≥ 1: 4 in serum are consistent with current or recent infection, and high titers (≥ 1: 32) signify an increased likelihood of extrapulmonary dissemination.