Epstein-Barr virus, was discovered in 1964, up to now was believed to be associated with many cancers such as nasopharyngeal cancer, gastric cancer and malignant lymphoma... This review focuses on analyzing the advantages and disadvantages of Epstein-Barr virus testing techniques and their clinical application in the treatment of Epstein-Barr virus - positive nasopharyngeal cancer patients according to the motto of personalized medicine.