The frequency of mutation in APC was 26.9%; in TP53 was 38.5%; in KRAS and PIK3CA was 11.5%; in BRAF, FBXW7, KMT2D was 3.8% Conclusion: We successful preparation of sequencing library and hybrid-capture sequence of 20 designed target genes, initially investigating genetic mutations in early stage colorectal cancer patients Keywords: colorectal cancer, next-generation sequencing, carcinoma, mutation