Results: The appropriate chromatographic conditions were found out for quantitative determination of glucosamine, using an Ascentic RP C8 column (250 x 4.6 mm; 5 µm), mixture of acetonitrile and phosphate buffer pH 3.0 containing triethylamine (35: 65, v / v) as eluent in isocratic mode, flow rate of 0.5 ml / min., and detection wavelength of 195 nm.