Recombinant strain is capable of bacillopeptidase F synthesis with fibrinolytic activity 16FU / ml and anticoagulant activity after 60 seconds at a concentration of 100ml/ml. Conclusion: The study has successfully created Bacilllus subtilis WB800N strain carrying gene expression recombinant bacillopeptidase F Recombinant strain is capable of bacillopeptidase F synthesis, in order to obtain enzymatic hydrolysis activity and activity against fibrin coagulation.