Dectecting the pattern of p53 gene mutations in breast cancer by DNA sequencing and immunohistochemistry methods may be help to assess the correlation between p53 gene mutations and p53 protein accumulation, the prognosis the survival as well as having suitable treatment for patients. Aims: - Identify the rate of p53 gene mutations (from exon 5 to exon 8) and p53 protein accumulation in the tissues of breast diagnosed adenocarcinoma by immunohistochemistry and DNA sequencing.