After 24, 48, or 72 hours of 3-MCPD administration, brain was fixed and stained immunohistochemically with c-fos antibody. Results: 3-MCPD at the only dose of 100 mg / kg induced c-fos expression, whereas 3-MCPD at doses of 10 mg / kg or 50 mg / kg did not. Conclusion: Toxicity of 3-MCPD on brain did not exert clearly at doses and methods investigated.