Result: There were statistical significant differences between the concentrations of glucose, LDL-cholesterol of samples centrifuged at 4000 revolutions for 3 min compared with those of specimens centrifuged at 3000 revolutions for 5 min and 3000 revolutions for 10 min (p<0.05), with different concentration values were 5.64%, 8.11%, 9.82% and 10.86%, respectively.