Experimental results optimize the chromatographic conditions with a flow rate of 1.5ml. min-1 thus reducing the running time of the samples, the chromatograms obtained meet the symmetry coefficient. At the same time, optimizing the processing of samples with a high fat sample should combine ultrasound and cooling, as the recovery efficiency increased significantly from 62, 27% to 99.66% with relative standard deviation% RSD=0.96 (<2.00%).