Diagnosis of Neonatal Cholestasis Total and direct bilirubin Liver tests Tests for metabolic, infectious, and genetic causes Liver ultrasonography Hepatobiliary scan Occasionally biopsy of liver, operative cholangiography, or genetic testing (See also the North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition and the European Society for Pediatric Gastroenterology, Hepatology, and Nutrition's 2017 guideline for the evaluation of cholestatic jaundice in infants.)