The study evaluated the in vitro antibacterial activity of HL solution against some pathogenic bacteria strains including both standard strains and clinical isolates i. e., Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus pneumoniae and Steptococcus pyogenes (Beta-hemolytic streptococci group A). The results showed that HL solution had inhibitory effect at whole concentrations (1) and some dilutions (1/2; 1/4; 1/8 and 1/16).