Technical parameters of multiplex-PCR: accuracy (AC) (dairy: 100%, aquatic products: 95% and meat: 91.67%); specificity (SP) (dairy: 100%, aquatic products: 96.67% and meat: 90%); sensitivity (SE) (dairy: 100%, aquatic products: 93.33% and meat: 93.33%). Conclusion: Application of multiplex-PCR for detection of pathogenic E coli (EPEC, ETEC, EHEC) in food and the food born outbreak.