Results: A simple HPLC method was developed for quantitative determination of neferine in total alkaloidal extracts from loti embryo with following chromatographic conditions: Phenomenex Lunar RP18 column (250 x 4.6 mm; 5 µm), mobile phase in isocratic mode including mixture of acetonitrile, water, and 60 mM phosphate buffer with pH adjusted to 7 (30: 45: 25), flow rate of 1 ml / min., detection wavelength of 282 nm.