And the appropriate time to acquire carotenoids for vegetative cells of Bacillus strains was examined in order to provide additional data for achieving simple and cost-effective carotenoid production. Methods: Six strains of carotenogenic Bacillus species consisting of B marisflavi (DD1.1), B vietnamensis (HC28), B infantis (AT14), B licheniformis (AT22), B indicus (HU36) and B firmus (CG17.0) were grown in tryptic soy broth to investigate growth curves and carotenoid content by time.