Methods: A molecular method was applied to identify H pumilo and H taichui based on amplification of ITS-2 using forward primer, 3SF: 5'-GGTACCGGTGGATCACTCGGCTCGTG-3' and reverse primer BD2R: 5'-TATGCTTAAATTCAGCGGGT-3-BD2R) with annealing at 50oC in the polymerase chain reaction (PCR) and sequencing for analyzing the nucleotide composition.