Diagnosis of Congenital and Perinatal CMV Infection Viral culture using urine, saliva, or tissue Polymerase chain reaction (PCR) testing using urine, saliva, blood, or tissue Symptomatic congenital CMV infection must be distinguished from other congenital infections, including toxoplasmosis, rubella, lymphocytic choriomeningitis virus (LCMV), and syphilis.