How To Do Suprapubic Aspiration of the Bladder in a Child By Keara N DeCotiis, MD, Nemours / Alfred I duPont Hospital for Children Reviewed / Revised Dec 2020 | Modified Sep 2022 View Patient Education Indications Contraindications Complications Equipment Additional Considerations Relevant Anatomy Positioning Step-by-Step Description of Procedure Aftercare Warnings and Common Errors Tips and Tricks Suprapubic aspiration is obtaining a urine sample using a needle inserted into the bladder in the suprapubic region, typically to obtain an uncontaminated urine sample for culture.