The study established a procedure for extracting and isolating Beauvericin (purity 98.1%) from Cordyceps cateniannulata CPA14V by using combination of different chromatographic methods such as: thin layer chromatography, normal-phase chromatogaraphy with silicagel stationary phase, reversed-phase chromatography with YMC RP 18 stationary phase and size exclusion chromatography with sephadex LH-20 stationary phase at laboratory scale.