Objectives: 1) To define the difference of LDL-cholesterol level quantification from Friedewald and Vujovic indirect methods to direct methods at Hoan My Minh Hai General Hospital in 04/2021 - 07/2021. 2) To define the linear correlate regression of LDLcholesterol level quantification from Friedewald and Vujovic indirect methods to direct method LDL-C Materials and methods: A cross-sectional descriptive study was carried out of 674 blood samples in patients indicated for cholesterol, triglycerides, HDL-C and LDL-C. The level of cholesterol, triglycerides, HDL-C and LDL-C were quantified by direct method, by the way, the LDL-cholesterol level was calculated by Friedewald and Vujovic indirect methods.