QRT-PCR reaction: Using Sybr Green and self-designed primers to operatereal-time PCR the miRNA-16, miRNA-146 - 3p, miRNA - 147b, miRNA-155, miRNA-223 8 Real-time PCRoperating condition: 500C - 2 minutes Repeat 1 cycle 950C - 10 minutes Repeat 1 cycle 950C - 15 seconds Repeat 45 cycles 580C - 1 minute - All reactions were performed on Agilent machines - Internal standard: MiRNA-16 had been selected as the internal standard - Quantitative miRNA assay was performed at the Department of Molecular Biology of 108 Military Central Hospital.