When planting red beet sprouts on the substrate No. 1, concentration GLS was 23.45 mg sinigrin/100 g fresh weight, SC50 was 395.07 µg / ml and crop yield was 1021.51 g. When adding the amino acid L-Tryptophan 50 mg, additional time on day 3 for GLS content and high antioxidant ability were 28.63 mg sinigrin / 100 g fresh weight and 290.8 µg / ml, respectively.