Experimental methods: Construction of pAX01-bpr and transformation of B subtilis WB800N, the recombinant strain was constructed by transforming plasmid pAX01-bpr into B subtilis strain WB800N to produce strain WB800N / pAX01-bpr, induced expression and information bacillopeptidase, quantitative fibrinolytic activity on fibrin plate and casein plate, determined fibrinolytic activity, determined the anticoagulant activity in vitro and expression analysis of extracellular bacillopeptidase from B subtilis strain WB800N by SDS - PAGE.