AFB and MTBC detection was determined by (1) Acid Fast Bacilli (AFB) screening by fluorecence microscopy, two conventional culture methods (gold standard) of Lowenstein-Jensen (LJ) and Mycobacterium Growth Indicator Tube (MGIT) and Drug Sensitivity Testing (DST) using MGIT method to detect INH and RIF resistance (gold standard); (2) The Genotype MTBDRplus assay for the detection of MTBC and its ability to detect resistance to Isoniazid and Rifampin.