Subject and method: A 1200ml normal plasma sample pool that was clear, light yellow, having serum indices values: < 0.25 index for hemolysis, < 30.0 index for icteria, < 0.3 index for lipemia, respectively and 3 types of plasma samples: 150ml plasma with artificial hemolysis, 150ml plasma with over 360μmol / L total bilirubin concentration, 150ml plasma adding 20% intralipid up to triglyceride concentration of 20 mmol/l.