Our study aim is exam the prevalence of seropositive and risk factors of pregnant women during first trimester at tu du hospital. Methods: A hospital based cross-sectional study conducted from July 1st, 2008 to May 30th 2009.Resulst: Conducted research on 380 pregnant women, mean age of participants was 28.31 ± 7.66 years, 3.7% case had IgM positive (95% CI: 1.8 - 5.6).