The results showed that compared with all pigs in the control group with typical clinical manifestations of Blue-ear disease, all inoculated pigs had normal body temperature and weight gain, besides, the S / P index increased from 1.65 ± 0.1 to 2.99 ± 0.2 (the first vaccine batch) and from 1.17 ± 0.1 to 2.08 ± 0.5 (the second vaccine batch); the average antibody titer was ≥ 2560, and virus was not detected in nasal fluid by real-time RT-PCR (rRT-PCR) from 7 days post challenge.