There were a statistically significant differences in the level of general discomfort according to the VAS scale, in which the VAS score of the acidophilic group (8.00±0.82) hadstatistically significantlyhigher than that of the neutrophilic group (6.67±1.21) and low cells (5, 67±1, 53) with p-values of 0.032 and 0.004, respectively.