About the culture results, regardless of the group of the S viridans, the detection rate of pathogens was 44.3%, lower than that of multiplex real-time PCR, in which the highest was K pneumoniae (12.2%), followed by P aeruginosa (9.6%), A baumannii (7.7%) and E coli (5.2%); No case of S pneumoniae, H influenzae and atypical bacteria was isolated.