In this study, the authors present the characteristics of Aconitum carmichaelii flavonoid 3 '5' hydroxylase (AcF3'5'H) gene isolated from mRNA of A carmichaelii plant, and the design of construct carrying AcF3'5'H transgene in plant expression vector to enhance AcF3'5'H gene expression in transgenic A carmichaelii Debx plants.