Results: Among synthesized benzohydrazide derivatives, two compounds (HDP 3C and HDP 3F) exhibited anti-acetylcholinesterase activity more potent than that of the reference of galanthamine Conclusion: Addition of the quaternary ammonium group via the N-benzylation of pyridinohydrazone enhances significantly the anti-acetylcholinesterase activity, particularly in the compounds of 4-methyl - (2-benzoylhydrazono) methyl) - 1-benzyl - pyridium.