Red cells, Buffy Coat Removed, in Additive Solution prepared from the top-bottom (350ml) blood bags system meet the quality criterias standard, average: volume 225.4±10.6 ml, hematocrit 62.4 ± 2%, hemoglobin 46.8 ± 2.7g, white blood cells count (X109) 0.75 ± 0.12, removed leukocyte rate 72.6 ± 4.4%.