Results: The minimum inhibitory concentrations of Alfacef 1g on 10 common standard bacteria, including three Gram-negative bacteria and seven Gram-positive bacteria: Klebsiella pneumoniae CF 104, Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, Enterococcus faecalis ATCC 51299, E coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, and Corynebacterium diphtheriae ATCC 51696; have been demonstrated the equivalence of antimicrobial activity, compared with those of brand name Fortum.