Toxicity was measured through activities, eating and drinking capacity of mice, weight, biochemical tests (AST, ALT, total cholesterol, triglyceride, creatinine, uric acid, glucose) and hematology tests (hematocrit, hemoglobin, mean hemoglobin, mean hemoglobin concentration, average red cell volume, white blood cell count, platelet count), weight of liver, weight of kidney, weight of spleen, liver and kidney microscope.