We hereby described and appđied immunofluorescence microscopy (IF) and combine IF with LM to obtain the final pathological diagnosis in 30 cases of glomerular diseases, including 4 cases of IgA nephropathy, 1 Henoch Schonlein nephritis, 2 membranoproliferative glomerulonephritis, 1 Alport syndrome, 1 end-staged kidney, 6 lupus glomerulonephritis, 9 minimal change nephrotic syndrome, 5 focal and segmental glomerulosclerosis, and 1 pauci-immune crescentic glomerulonephritis.