Peripheral blood was collected from the subjects to analyze the T lymphocyte (CD3+), TCD4 lymphocyte (CD3+CD4+CD8 -), TCD8 lymphocyte (CD3+CD4-CD8+), B lymphocyte (CD3-CD19+) and NK cell (CD3-CD16&56+) counts by flowcytometry method on BD FACS Canto-II machine using BD Multitest 6 color TBNK KIT.