Results: This study investigated on 135 positive samples of non-fermenting Gram-negative bacilli isolated from blood, sputum, pus, urine and other body fluids samples. The rates of A baumannii is 32.6%, P aeruginosa 28.1%, B cepacia 22.2%, S maltophilia 3.7%, Burkholderia pseudomallei 2.2%, Chryseobacterium meningosepticum 1.5%, and the same rates of non-fermenting Gram-negative strains 0.7%, included Pseudomonas alcaligens, Pseudomonas fluorescens, Pseudomonas luteola, Brevundimonas vesicularis, Weeksella virosa.