Results: The suitable chromatographic conditions for quantitation of (E) - 10-hydroxy-2-decenoic acid were obtained using a stainless steel column (250 x 4.6 mm), packed with octadecylsilane bonded to porous silica (5 mm); mixture of methanol and phosphoric acid pH3 (55: 45) in isocratic mode as mobile phase; flow rate of 1 ml per minute, injection volume of 10 µl; column temperature at 30 oC and detection wavelength at 212 nm.