Results: The appropriate chromatographic condition were found out for simultaneous quantitative determination of methyl paraben and propyl paraben, using a Luna C18 (150 × 4.6 mm; 5 µm), mixture of methanol and phosphate buffer (55: 45, tt / tt) as eluent in isocratic mode, flow rate of 1.0 ml / min, and detection wavelength of 272 nm and column temperature of 40 0C.