Objectives: This research aim was to study the permeation of scopolamine hydrobromide through rat skins with the impact of enhancer, from which the most appropriate enhancer would be considered and chosen to enhance the ability of permeation of scopolamine hydrobromide and to be function as a fundamental for the study of drug-in-adhesive patch (DIA - patch). Methods: The effect of enhancer through rat skins has been in a survey by the rubbing the saturated solution of scopolamine hydrobromide in enhancer were evaluated using Franz diffusion cell fitted with excised rat skin.