Recently, MLPA is described as an effective method to dectect these mutations. Objective: Evaluate the effectiveness of MLPA in detecting DMD gene deletion mutations. Method: 15 DNA samples of male affected with DMD / BMD detected with deletion mutations by multiplex PCR are tested for 79 exons with Salsa MLPA P034A2 và P035A2 kit, capillary electrophoresed with CEQ 8000 and automated analysis with Gene Marker software. Results: MLPA allows to detect the size of DNA fragment lost, the technique is easy, visual, rapid and reproducible.