Means helping to definitely diagnose pulmonary tuberculosis AFB (-), ranked in descending order, were as follows: expert opinions 59.8%; sputum LPA 13.7%; sputum Xpert MTB / RIF 12.6%; sputum MGIT culture 4.2%; LPA of bronchial wash fluid 4.0%; and MGIT culture of bronchial wash fluid 3.8%.