Maternal age and quality of transfered embryo also affect the number of gestational sacs of the patient, the rate of multiple pregnancies increased in the younger age group (37.4% in the group < 35 years old and 28.6% in the group ≥ 35 years old), the incidence of multiple pregnancy in the group with only 1 embryo of grade 1 or 2 decreased 0.654 times than the group with both embryos of grade 1 and 2.