Results: the HPLC condition was optimized as follow: Eclipse XDB-C8 (4.6 mmx150 mm, 5µm) column; mobile phase: methanol, acetonitrile and 0, 057 mM acid triflouroacetic (pH 4.5) (0: 13: 87 - 10: 20: 70 - 0: 13: 87, v / v / v) in 40 mins;, examinized wavelength: 396 nm.