Thus, real-time PCR technique was born to quantify HBV-DNA more accurately, more efficiently and faster and has been applied in many places in the country. Research Objective: the technical set up real-time PCR study with the self building standard curve to quantify HBV-DNA in the pre-S region 89bp in the patient's blood sample tested for hepatitis B Research Methodology: The descriptive study was conducted on 46 patients infected with hepatitis B. All patients were quantified HBV-DNA by real-time PCR technique with the self building standard curve of Biochemistry - Molecular Biology department of Phạm Ngọc Thạch University of Medicine.