The influencing factors were evaluated by software FormRules v2.0 and the optimal formula predicted by the INForm v3.1 was optimized. The selected optimal formulation of rotundin sulfate tablets for a drug release process lasting 8 hours contained Metholose 100.000 RS 120 mg; Avicel PH101 24.0 mg; lactose 34.5 mg; and 8 kP tablet hardness.