Methods: A prospective, cross - sectional study was conducted from 2017 to 2018 in Ho Chi Minh city, a total of 183 ESBL-producing E coli isolates, in which 29 isolated from healthy individuals and 154 isolated from clinical specimens were tested for 19 virulence genes including: fimH, fyuA, traT, iutA, PAI, kpsMTII, papC, hlyA, kpsMTK1, cnf1, afa, ompT, hlyF, iroN, ireA, ibeA, sfa, iss and cvaC by a series of five multiplex-PCR sets and three monoplex - PCR using specific primer pairs.