and MICs ex vivo were determined by modified model using the experimental tinea unguium to evaluate the antifungal activity against Trichophyton rubrum and T mentagrophytes. Outcome: Data received from these experiments show the antifungal activities of 2-methoxy-1, 4 naphthoquinone against Candida spp. were equivalent to balsam extract. Conclusion: 2-methoxy-1, 4 naphthoquinone could be studied further more in vivo to use as an antifungal agen