Comparison of A3 method and bacterial cell count method on environmental samples of bed surface, bedside cabinets of patients, surgical table, ventilator surface showed that A3 method is twice as sensitive (75% with 36.3%); With the samples of surgeons hands, endoscopic instruments: The results are almost similar between the two methods (12.5% with 10%) (45.7% with 47.8%); Conclusions: Environment cleaning surveillance is an important in all hospitals to reduce hospital association infection.