Background The number of labor employers at Binh Duong province complying with the regulations of controlling working environment and health check-up for workers is limited, and little is known of the motivation underlying these practices. Objectives To determine factors associated with the compliance with the regulations of controlling working environment and health check-up for workers among labor employers at Binh Duong province in 2009.Methods A case-control study was conducted among the employers having had a working environment measure and a health check-up for their workers by the Center for Occupational and Environmental health at Binh Duong province in 2009.