A 37-year-old male with a fever (38 C) presented to the fever clinics with a 2-month history of intermittent low-grade fever, chills, hunger, and headache. About a month back, he underwent root canal therapy for pulpitis without receiving antibiotic prophylaxis. He developed a fever the night after the operation and was treated with tinidazole combined with cephalosporin for empirical anti-infection. After 1 week of treatment, the temperature returned to normal but the fever recurred 2 days after stopping the medication, which was not taken seriously. At this visit to our hospital, the white blood cell (WBC) counts were 9.67 x 109/L, neutrophil counts were 7.74 x 109/L, and the C-reactive protein (CRP) level was 51.01 mg/L. The value of the N-terminal B-type natriuretic peptide precursor (NT-proBNP) was as high as 159 pg/mL, so the patient was transferred to our department for further diagnosis and treatment.
He reported eating normally, regular bowel movements. He did not suffer from hypertension, diabetes, heart disease, cerebrovascular disease, hepatitis, history of tuberculosis, history of trauma, blood transfusion, intrathoracic, intracardiac procedures, or gastrointestinal tract pathology. He also did not have any history of intravenous drug use or smoking.
On physical examination, the patient was afebrile and sane. His blood pressure was 150/45 mmHg, with a heart rate of 104 bpm. There was no bump in the precordial area, the cardiac boundary was not enlarged, the heart sound was strong, the rhythm was uniform, and the level 2 wind-blowing diastolic murmur could be understood in the precordial area without a pericardial murmur. His skin was normal without rashes or subcutaneous bleeding, and his ophthalmoscopic examination was negative. Hearing and smell were normal. Oral hygiene was good, and the oropharynx was very healthy. Auscultation of the chest revealed clear lung fields bilaterally without any adventitious sounds. Physical examination revealed no abnormality in the chest, lungs, abdomen, liver, spleen, limbs, or intestines. It may be due to the brief duration of the disease that there were no common endocarditis signs, such as Osler node, Janeway lesion, or Roth spot.
Laboratory workup was notable for a hemoglobin level of 123 g/L, hypersensitive C-reactive protein (HS-CRP) of 57.65 mg/L, and an erythrocyte sedimentation rate of 58 mm/h on the day of admission to our department. We followed the blood routine examinations and cardiac function indexes on the second and third days. The WBC counts were 9.65 x 109/L and 9.91 x 109/L, neutrophil counts were 7.81 x 109/L and 7.42 x 109/L, and CRP levels were 71.65 and 48 mg/L. The NT-proBNP levels reached as high as 2,173 pg/mL. Transthoracic echo was notable for the aortic valve bivalve malformation, abnormal echo, and severe regurgitation left atrial dilatation, moderate mitral regurgitation, and left ventricular ejection fraction (LVEF) of 66% (Figure 1). So, aortic valve vegetation was considered. The longest was about 10 cm, and IE was highly suspected. Chest computed tomography (CT) examination showed no obvious abnormality.
On pathogen examination, we timely collected blood samples from patients for culture. To obtain etiologic results quickly, we performed mNGS detection with the patient's consent. On the second day, the results of feedback on specific pathogen tests showed that the 1,3-beta-D-glucan (G) and galactomannan (GM) tests were negative. The respiratory-tract inspection was negative, and the Coxsackie virus antibody was negative. EBV and CMV were identified by multiplex polymerase chain reaction (PCR), and the results were also negative. The serological tests were negative, and the rubella virus and Toxoplasma gondii serological tests were also negative. The mNGS results from feedback within 24 h showed that 63 reads of G. morbillorum were detected in the cfDNA of blood samples (Table 1). On the fourth day, the anaerobic blood culture bottle showed Gram-positive cocci, so the possibility of G. morbillorum was high. At this point, our patient met "diagnosed" endocarditis per Duke's criteria, and the pathogen was confirmed as G. morbillorum.
A total of 200 muL of plasma was used to extract the nucleic acid for cfDNA libraries.
Nextseq CN500 was used to perform sequencing after the library was validated by the 2100 Bioanalyzer system (Agilent Technologies, Inc.) and RT-PCR.
Sequence reads were classified according to their origin by a developed bioinformatics pipeline.
According to the Illumina next-generation sequencing assay manual, blood samples were processed in a medical laboratory (Nextseq CN500). The general detection process was as follows: 
Remove low-quality reads.
Host reads were subtracted.
The remaining reads were aligned to the reference database, composed of multiple public sequence resources of bacteria, viruses, and fungi.
The bioinformatics process mainly included the following steps: 
The patient showed fever, so we checked whether it was rheumatic fever, sepsis, systemic lupus erythematosus, and autoimmune diseases. The patient was excluded for rheumatic fever because there was no history of upper respiratory tract infection, joint pain, or rheumatic heart disease. Similarly, this patient did not meet the clinical symptoms of sepsis, i.e., high fever, chills, and obvious respiratory, digestive, urinary, and other infectious symptoms. In patients with systemic lupus erythematosus, pericarditis is the most common cardiac involvement; valve involvement is less, and most patients are female and excluded. Regarding autoimmune diseases, the anti-nuclear antibody spectrum of the patients was examined and all of them were negative. Then, we improved the results of echocardiography and etiological examination as soon as possible to ascertain whether it was IE.
Once a transthoracic echo revealed suspected endocarditis, our patient was empirically treated with ceftriaxone (2 g every 24 h was initiated) and netilmicin (2 g every 24 h was initiated). This decision was supported when the mNGS resulted in G. morbillorum, and blood cultures grew Gram-positive cocci. Because the drug sensitivity information could not be obtained through culture, we continued to use ceftriaxone for anti-infection treatment in combination with the literature and the patient's situation. Simultaneously with the anti-infection, as the patient showed rapid heart rate, enlarged left atrium, and elevated NT-proBNP, we considered that the IE might involve the valve to cause heart failure, and torasemide diuresis was given to correct heart failure. Due to the increased activity of aortic vegetation in patients, we should be vigilant against systemic embolism caused by the shedding of vegetation.
After 6 days of treatment in our department, the patient's condition appeared stable without any particular discomfort. The general situation worked well. The vital signs showed that the body temperature was 36.4 C, the blood pressure was 140/60 mmHg, the heart rate was 84 bpm, and the blood routine test results showed that the fluctuation range of various indicators was small. The CRP decreased to 21.83 mg/L. After only 1 week in the hospital, the patient firmly asked to be discharged. Blood was collected and cultured the day before discharge again, and sterile growth occurred 5 days later. No antibiotics were prescribed at discharge. We repeatedly explained the necessity of continued hospitalization and suggested that the patient receive cardiac surgical treatment as soon as possible. On the second day after discharge, the patient was sent to a top-grade hospital in Beijing for surgical treatment. At the follow-up, the patient was receiving preoperative intravenous antibiotic therapy. Unfortunately, we could not obtain the antibiotic information.