IL-10-producing B cells , CD4 ( + ) / CD25 ( high ) Foxp3 ( + ) , and CD8 ( + ) / CD28 ( - ) Foxp3 ( + ) Tregs , CCR6 ( + ) / CD123 ( + ) / IDO ( + ) DCs , as well as Th17A and Th22 cell subpopulations were quantitated by flow cytometry .