Real-time PCR and Western blot analysis of the expression of the endoplasmic reticulum stress marker HSPA5 and of selected genes representing the three pathways of unfolded protein response ( IRE-XBP1 , PERK-ATF4 , ATF6p90-p50 ) in inflamed and uninflamed biopsies from 28 inflammatory bowel disease paediatric patients and 10 controls .