Plasma glucagon levels ( AUC0-24h 1,533 656 vs. 1,120 344 ng/L/h , P = 0.027 ) and lipolysis ( free fatty acid AUC0-24h 7.5 1.6 vs. 8.9 1.9 mmol/L/h , P = 0.005 ; - OH-butyrate AUC0-24h 6.8 4.7 vs. 17.0 11.9 mmol/L/h , P = 0.005 ; glycerol , P < 0.020 ) were overall more suppressed after evening versus morning glargine administration .