The frequency of PD-1 + CD8 T cells in all of the CHB patients was significantly correlated with both the HBV DNA level ( r = 0.45 , P = 0.01 ) and HBeAg level ( r = 0.47 , P = 0.01 ) at treatment week 24 , but the frequency of Treg cells was only significantly correlated with the HBeAg level ( r = 0.44 , P = 0.02 ) .