Increases in IgG binding to insulin , saliva IgA binding to insulin , or CD4 + T-cell proliferative responses to insulin were observed in 2 of 10 ( 20 % [ 95 % CI , 0.1 % -45 % ] ) placebo-treated children and in 1 of 6 ( 16.7 % [ 95 % CI , 0.1 % -46 % ] ) children treated with 2.5 mg of insulin , 1 of 6 ( 16.7 % [ 95 % CI , 0.1 % -46 % ] ) treated with 7.5 mg , 2 of 6 ( 33.3 % [ 95 % CI , 0.1 % -71 % ] ) treated with 22.5 mg , and 5 of 6 ( 83.3 % [ 95 % CI , 53 % -99.9 % ] ) treated with 67.5 mg ( P = .02 ) .