Further analysis revealed a significant reduction of tick infestation by permethrin treatment only for subjects wearing trousers without the cut protection lining ( I-2 : 34.2 vs. II-2 : 80.0 % , p < 0.001 ) , while users of cut protection trousers did not benefit from such treated trousers ( I-1 : 38.6 vs. II-1 : 47.6 % , n.s. ) .