The aims of this study were to transfer and validate an HPLC-MS/MS methodology on a UPLC-MS/MS new generation platform , using an HPLC column , for urine analysis of treated and untreated pediatric and adult Fabry patients , to establish correlations between the excretion of Fabry biomarkers with gender , treatment , types of mutations , and to evaluate the biomarker reliability for early detection of pediatric Fabry patients .