In two double-blind , randomized , crossover studies , 18 Japanese participants ( aged 20-65years ) and 24 European participants ( aged 18-65years ) with glycated haemoglobin levels 9.0 % ( 75mmol/mol ) received single subcutaneous doses of Gla-300 , 0.4 , 0.6 and 0.9 U/kg ( 0.9 U/kg in the European study only ) , and Gla-100 , 0.4 U/kg .