Mean changes between groups using generalized estimating equations and examined effects of weight-loss on LINE-1 methylation using stratified analyses ( gained weight/no weight-loss [ N = 84 ] ; < 5 % [ N = 45 ] ; 5 % -10 % [ N = 45 ] ; > 10 % of baseline weight-loss [ N = 126 ] ) within each arm , adjusted by blood cell counts were compared .