The blood sample was drawn , and body temperature , routine blood test , C-reactive protein ( CRP ) , liver and kidney function , fasting glucose ( Glu ) , and serum interleukin-6 ( IL-6 ) , high mobility group protein B1 ( HMGB1 ) levels were determined in all the patients before anesthesia ( T1 ) , at the end of operation ( T2 ) , 12 hours after operation ( T3 ) , or at 5:00 am on the third day after operation ( T4 ) .