Assessment included lesion counts in two 33-cm regions of interest ( ROIs ) , one on each side of the scalp ( ROI-1 : tUVB weeks 0-16 , ROI-2 : tUVB weeks 9-16 ) , patient self-assessment and analysis of MMP1 , MMP9 , TGFB1 and COL1A1 mRNA expression by quantitative reverse-transcription polymerase chain reaction .