A total of 73 subjects ( 38 males and 35 females ; aged 25-60years ) were randomly divided into two groups : Group 1 - a placebo dentifrice ( The Himalaya Drug Company Research and Development , Makali , Bangalore ) and Group 2 - ( test group ) , a commercially available herbal dentifrice ( Hi Ora K , The Himalaya Drug Company Research and Development , Makali , Bangalore ) .