Those patients were divided into 2 groups randomly : 260 cases in intradermal injection group were administered via bilateral forearm intradermal injections for immunotherapy once 21 days , then the blocking antibody was determined after 2 ( 23 cases ) , 3 ( 73 cases ) , 4 ( 74 cases ) , 5 ( 90 cases ) times respectively , while in subcutaneous injection group , the 66 cases were administered via subcutaneous injection once 21 days , the blocking antibody measured after 3 times ; In both cases , the blocking antibody was all determined 2 weeks later .