BMI and WHR were calculated for patients at the beginning and end of the study and blood samples were obtained from the patients to measure serum concentrations of aspartate aminotransferase ( AST ) , alanine aminotransferase ( ALT ) , fasting serum glucose ( FSG ) , insulin resistance ( IR ) , vaspin , chemerin , pentraxin 3 ( PTX3 ) and markers of oxidative stress including total antioxidant capacity ( TAC ) and malondialdehyde ( MDA ) .