A total of 356 RhD-positive and 44 RhD-negative samples were tested for the ( C ) ce ( s ) haplotype using two allele-specific primer polymerase chain reactions that detect c. 733C > G ( p.Leu245Val ) and c. 1006G > T ( p. Gly336Cys ) substitutions in exon 5 and 7 of the RHCE gene .