Fasting ( midpoint and endpoint ) and postprandial blood at the endpoint following a test meal ( 3.54 MJ , 14g protein , 85g carbohydrate and 50g fat as PO ) were collected for the measurement of C-peptide , insulin , glucose , plasma glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 , lipids and apolipoproteins ; pre-specified primary and secondary outcomes were postprandial changes in C-peptide and plasma glucose .