We measured plasma and urine concentrations of both QGC001 and EC33 by liquid chromatography-tandem mass spectrometry , plasma renin concentrations ( PRC ) , plasma and free urine aldosterone ( PAldo and UAldo ) , plasma copeptine ( PCop ) , and plasma and urine cortisol ( PCort and UCort ) concentrations , and supine systolic blood pressure ( SBP ) , diastolic blood pressure ( DBP ) and heart rate ( HR ) at various time points .