All the subjects performed two treadmill ergoespirometry tests : first , a modified Bruce protocol to discard ischemic responses during exercise and to evaluate VO2max before the experimental phase ; and second , with an eccentric protocol ( 14 % descending ramp test ) during 10 minutes in stable state at 70-80 % VO2max , as experimental inflammatory protocol.We compared intra and inter groups to evaluate differences in the pre and post-test differences results on blood muscle damage variables .