The exclusion criteria are as follows : ( 1 ) pregnancy , ( 2 ) diabetes mellitus , ( 3 ) tobacco use , ( 4 ) alcohol abuse , ( 5 ) evidence of nonchagasic heart disease , ( 6 ) depression , ( 7 ) dysphagia with evidence of food residues in the esophagus , ( 8 ) dysphagia with weight loss higher than 15 % of usual weight in the last four months and/or ( 9 ) conditions that may result in low protocol adherence .