1 ) Cycling ( C ) , 2 ) Cycling with superimposed E ( C+E ) and 3 ) E. Interleukin-6 ( IL-6 ) , brain-derived neurotrophic factor ( BDNF ) , creatine kinase ( CK ) and myoglobin were determined before ( pre ) and 0 ' , 30 ' , 60 ' , 240 ' and 24h after each intervention .