Ileal infusion of lipid , protein and carbohydrate resulted in a significant reduction in food intake compared with control ( HL : 464.390.7 kcal , P < 0.001 ; HP : 458.078.6 kcal , P < 0.005 ; HC : 399.057.0 kcal , P < 0.0001 vs control : 586.770.2 kcal , P < 0.001 , respectively ) .