Reverse transcriptase gene amplification was successful in 128/169 ( 76 % ) individuals and 95 % of HIV-1 were infected with subtype B. Rilpivirine RAMs were detected in 41 ( 32 % ) individuals , with highest frequency for the mutations Y181C/I/V ( 18 % ) , K101E/P ( 7 % ) and E138A/G/K / Q/R/S ( 6 % ) and the association L100I + K103N/S ( 5 % ) .