Serologies for HIV ( using parallel rapid tests ) , herpes simplex virus type 2 ( HSV-2 ; using Focus HerpeSelect IgG2 ELISA [ Focus Technologies , Cypress Hill , CA ] ) , and syphilis ( rapid plasma reagin confirmed by Treponema pallidum hemagglutination ) were determined , with plasma HIV-1 RNA and CD4 count in HIV-positive patients .