In a randomized , parallel-design , controlled trial of 32 men and 7 women , volunteers were assigned diets providing protein at 0.8 [ Recommended Dietary Allowance ( RDA ) ] , 1.6 ( 2 RDA ) , or 2.4 ( 3 RDA ) g kg ( -1 ) d ( -1 ) for 31 d. Ten days of WM preceded 21 d of ED , during which total daily ED was 40 % , achieved by reduced dietary energy intake ( 30 % ) and increased physical activity ( 10 % ) .