In large follicles , hCG supplementation induced a nearly 3-fold increase of estradiol ( nanomoles per liter ) [ D0 : 1496 ; D50 : 3138 ; D100 : 4338 ; D150 : 4009 ( P < .001 ) ] , a significant 3-fold increase of androstenedione , and a 5-fold increase of T ( nanomoles per liter ) [ D0 : 15 ; D50 : 38 ; D100 : 72 ; D150 : 56 ( P < .001 ) ] .