Subjects with type 2 diabetes attended for baseline evaluation including BMI , glycaemic and lipid markers , endothelial function ( photoplethysmography ) , insulin resistance ( HOMA ) , inflammation ( sVCAM-1 and Hs-CRP ) and markers of oxidative stress [ total anti-oxidant status ( TAOS and FRAP ) , whole blood total glutathione ( GSH ) assays ] .