The arterial blood was withdrawn before TAES ( T0 ) , before anesthesia ( T1 ) , before cutting ( T2 ) , at 60 min after encephalic incision ( T3 ) , immediately after incisions suture ( T4 ) , at about 10 min after removing tracheal catheters ( T5 ) to detect beta-endorphin ( beta-EP ) , cortisol ( COR ) , adrenalin ( E ) , blood sugar ( Glu ) .