Treatment duration ( d ) for the in vivo groups was d 60 mJ > d 100 mJ > d Ultrasound = d 160 mJ > d 250 mJ ( P 0.046 ) , while for the in vitro groups it was d 60 mJ > d 100 mJ = d Ultrasound = d 160 mJ > d 250 mJ ( P 0.046 ) .