At baseline and 3 hours after treatment , we assessed ( 1 ) flow-mediated dilation of brachial artery ; ( 2 ) coronary flow reserve , ejection fraction , systemic arterial compliance , and resistance by echocardiography ; ( 3 ) left ventricular global longitudinal and circumferential strain , peak twisting , untwisting velocity by speckle tracking ; and ( 4 ) interleukin-1 , nitrotyrosine , malondialdehyde , protein carbonyl , and Fas/Fas ligand levels .