The total effective rates were 40.0 % ( 10/25 ) , 64.0 % ( 16/25 ) and 76.0 % ( 19/25 ) in the observation group and were 59.1 % ( 13/22 ) , 68.2 % ( 15/22 ) and 77.3 % ( 17/22 ) in the control group in 30 days , 60 days and 90 days after treatment respectively .