Main outcome measures were body composition by DXA and CT , bone marrow fat by proton MR spectroscopy , P1NP , CTX , 25 ( OH ) D , hsCRP , undercarboxylated osteocalcin ( ucOC ) , preadipocyte factor 1 ( Pref 1 ) , apolipoprotein B ( ApoB ) , and IGF-1 .