His pupils included Emperor Goyozei's brother Prince Hachijonomiya Toshihito, and court nobles like Michikatsu NAKANOIN and Mitsuhiro KARASUMARU; Teitoku MATSUNAGA and Choshoshi KINOSHITA also received instruction from Yusai.
門人には後陽成天皇の弟・八条宮智仁親王、公家の中院通勝、烏丸光広などがおり、また松永貞徳、木下長嘯子らも幽斎の指導を受けた。