The Miyoshi triumvirate tried to resist Nobunaga's visit to the Imperial Court in Kyoto in 1566 but failed, marking the decline of their power, and Tomomichi died in battle during the Genki Period while the others disappeared without a trace, then with the declining power of Miyoshi clan in Kinai region, the activities of Miyoshi Triumvirate signified the complete end.
1566年、織田信長の上洛に反発し、抵抗するが敵せず相次いで敗退し、三好三人衆の勢力は衰え、元亀年間には友通が戦死し、他の二人も消息不明な状態となり、畿内における三好氏の勢力の衰退と前後して三好三人衆としての活動は完全に途絶えた。