The course of Line No. 25 to Uji Shako was nearly the same as that of the current Line No. 21, and its northward course from Uji Shako extended to Shinden, to Obaku (current Obaku Station,) to Kowata Yamate-cho, to Machinami-cho (later Machinami [Shodai or the first generation - currently JR Rokujizo]) and to Keihan Rokujizo.
25系統の経路は宇治車庫までは現行21系統とほぼ同一、宇治車庫以北は宇治車庫～新田～黄檗（現・黄檗駅）～木幡山手町～町並町（後の町並（初代）。現・JR六地蔵）～京阪六地蔵であった。