The Gohojo clan in the Kanto Region and the Date clan in the Tohoku Region signaled their allegiance to Nobunaga and, as a result, enemies that stood in Nobunaga's way were the Mori clan in the Chugoku Region, the Chosokabe clan in the Shikoku Region, the Uesugi clan in the Hokuriku Region and the Shimazu clan in Kyushu.
関東地方の後北条氏、東北地方の伊達氏は信長に恭順する姿勢を見せており、これで信長の目の前に立ちはだかる敵は、中国地方の毛利氏、四国の長宗我部氏、北陸地方の上杉氏、九州の島津氏となった。