Mitsuhide and his wife had two daughters, Nobusumi ODA's wife and Tadaoki HOSOKAWA's wife Garasha (Christian name Gracia) HOSOKAWA, and a son, Mitsuyoshi (Jugoro) AKECHI.
2人の間には、織田信澄室、細川忠興室細川ガラシャ（洗礼名ガラシャ）、嫡男明智光慶（十五郎）がいる。