The members from the Mito clan; Kamo SERIZAWA, Nishiki NIIMI (Iori TANAKA), Jusuke HIRAMA, Goro HIRAYAMA, Shingoro KASUYA, Jusuke HIRAMA, Kenji NOGUCHI, the members from Shieikan training hall; ABIRU, Isami KONDO, Keisuke YAMANAMI, Toshizo HIJIKATA, Shinpachi NAGAKURA, Sanosuke HARADA, Soji OKITA, the members who joined in Kyoto including Genzaburo INOUE, Hajime SAITO and Matasaburo SAEKI, and NEGISHI force including Yuzan NEGISHI and Goichi SHIMIZU came together.
芹沢鴨・新見錦（田中伊織）・平山五郎･粕谷新五郎・平間重助・野口健司ら水戸藩出身者、阿比類、近藤勇・山南敬助・土方歳三・永倉新八・原田左之助・沖田総司・井上源三郎ら試衛館道場の者、斎藤一・佐伯又三郎ら京で参加したもの、根岸友山・清水吾一ら根岸派が結集する。