After that, the medicine certification examination system was introduced in 1875, but Ine did not have qualification of candidacy for an examination because she was a woman, and she was obliged to closed her clinic in Tokyo and returned to her hometown, Nagasaki.
その後、1875年（明治8年）に医術開業試験制度が始まり、女性であったイネには受験資格がなかったため、東京の医院を閉鎖、郷里・長崎に帰郷する。