However, enmity of old retainers of the Tsutsui clan against him remained, and he was assassinated by the Yamanaka clan, who were former colleagues in Fushimi on April 3, 1609.
しかし、筒井氏旧臣の恨みはおさまらず、慶長14年（1609年）2月29日、伏見でかつての同僚であった山中氏に暗殺された。