However in 1865, navies from various overseas countries thought the cause of the exclusionism movement was due to the existence of Emperor Komei, and they sent a fleet to Osaka Bay and demanded Imperial sanction of the Emperor for the agreement, finally the Emperor realized how serious the situation was, he decided to issue an Imperial sanction of the agreement.
しかし慶応元年（1865年）、攘夷運動の最大の要因は孝明天皇の存在にあると見た諸外国海軍は艦隊を大坂湾に入れて天皇に条約の勅許を要求して、天皇も事態の深刻さを悟って条約の勅許が出される事となった。