Starting with Saichiro INOUE, who was the shita metsuke (low class inspector of foot soldiers) of the Tosa Domain, Izo assassinated, in the name of heaven's punishment, Seiichiro HONMA who was his comrade, Daigaku IKEUCHI, Magoroku MORI, Juzo OGAWARA, Kinzan WATANABE, and Jonosuke UEDA, who were government officials and yoriki (police sergeants) that belonged to the Kyoto City Magistrate, Tatewaki TADA who was the son of Kazue MURAYAMA (she was tied to a bridge and made a public display alive), a mistress of Shuzen NAGANO (who commanded Ansei no Taigoku [suppression of extremists by the Shogunate]).
土佐藩下目付けの井上佐一郎をはじめ、同志の本間精一郎や池内大学、森孫六・大川原重蔵・渡辺金三・上田助之丞などの京都町奉行の役人や与力、長野主膳（安政の大獄を指揮した）の愛人村山加寿江の子多田帯刀などを天誅と称して暗殺（村山加寿江は橋に縛りつけられ生き晒しにされた）。