In the Muromachi period, until the generation of Yoshihisa ASHIKAGA, the House of Hino was related to the House of Ashikaga shogunate by marriage: the House of Hino produced wives of the shogun for four generations, including Nariko HINO and Yasuko HINO (a niece of Nariko), who became wives of the third shogun Yoshimitsu ASHIKAGA of the Muromachi shogunate; Eiko HINO (younger blood sister of Yasuko), who became a wife of the fourth shogun Yoshimochi ASHIKAGA; sisters Muneko HINO and Shigeko HINO, who became wives of the sixth shogun Yoshinori ASHIKAGA, and Tomiko HINO, who became a wife of the eighth shogun Yoshimasa ASHIKAGA.
また、室町時代には足利義尚の代まで足利将軍家と結縁し、室町幕府3代征夷大将軍足利義満の室となった日野業子・日野康子（業子の姪）、4代足利義持の室となった日野栄子（康子の実妹）、6代足利義教の室となった日野宗子・日野重子姉妹、8代足利義政の室となった日野富子と4代の将軍の御台所を輩出している。