Yoshinobu TAKEDA, who was a Shingen's heir as well as a husband of Ujizane's younger sister, Reisho-in, opposed this, but in 1565, Shingen incarcerated Yoshinobu for the crime of treason, disinherited him, and annulled the marriage alliance.
信玄の嫡男で氏真の妹・嶺松院の夫である武田義信はこれに反対したが、信玄は永禄8年（1565年）に義信を反逆の罪で幽閉して廃嫡し、婚姻同盟を破棄した。