The campus was placed in the area stretching from Oaza Taishogun, Kinugasa-Village, and Kadono County (present-day, Sakata-cho, Taishogun, Kita Ward, Kyoto City), which it took over from its predecessor, Kyoto Sangyo Koshu-jo (Kyoto Training Institute of Sericulture), to Hanazono-Village (present-day, Takatsukasa-cho, Hanazono, Ukyo Ward, Kyoto City).
前身の京都蚕業講習所から引き継いだ、葛野郡衣笠村大字大将軍（現・京都市北区大将軍坂田町）から花園村（現・京都市右京区花園鷹司町）にかけての校地を使用した。