In response to this, the Shiei-kan and Mito Parties, along with Yuzan NEGISHI and his group controlled by TONOUCHI, remained in Mibu Village of Kyoto, but the NEGISHI party withdrew immediately afterwards, TONOUCHI and IESATO were excluded, and in March of the same year "Mibu Roshi-gumi" (also known as "Seichu Roshi-gumi") was formed.
これに応えて試衛館派、水戸派、殿内以下、根岸友山一派などが京の壬生村に残ったが、根岸派は直後に脱退、殿内・家里は排斥され、同年3月、新選組の前身である「壬生浪士組」（「精忠浪士組」とも）が結成される。