It was Naokatsu II who succeeded as head of the family, however, after Naokatsu retired due to poor health, his little brother Naotaka II took over and his descendants became the lord of the Hikone domain.
家督は長男の井伊直勝が継いだが、病弱であったため、家康の命により、直勝は隠居して直勝の弟・井伊直孝が家督を継ぐこととなり、直孝の子孫が彦根藩主を継承することとなる。