In March, following the bakufu order, Kondo renamed himself Takeshi OKUBO, restructured the troop as the Koyo Chinbutai troop, and led the troop to Kofu for service, but the troop lost to the new government force in the Battle of Koshu-Katsunuma and he was routed, and at the same time, Shinpachi NAGAKURA and Sanosuke HARADA left the troop because of conflicts of opinion.
3月、幕府の命を受け、大久保剛と改名した近藤は甲陽鎮撫隊として隊を再編し甲府へ出陣したが、甲州勝沼の戦いで新政府軍に敗れて敗走し、その際、意見の対立から永倉新八、原田左之助らが離別する。