Around the same time as raising an army in Ishiyama, another Ikko Ikki started from Nagashima Gansho-ji Temple (Nagashima Ikko Ikki) which exhibited its hostility against Nobunaga by destroying Nobunaga's younger brother Nobuoki ODA's Kokie-jo Castle in Owari Province, and, in June 1571, Nobunaga tried to exterminate Nagashima but failed with a lot of soldiers lost.
石山挙兵とほぼ同時に長島願証寺で一向一揆が発生（長島一向一揆）し、信長の弟織田信興が守る尾張の小木江城を滅ぼすなどして公然と信長に敵対するようになると、元亀2年（1571年）5月に信長は長島殲滅を図るが失敗し、多数の兵を失った。