Daigaku Nanko (the Southern College of the University, a predecessor of the University of Tokyo), Kaiseijo and Kounkan became middle schools which belonged to the 1st university district, the 4th university district and the 6th university district respectively, and Daigaku Toko (the Eastern College of the University), Osaka Igakko (Osaka Medical School) and Nagasaki Igakko (Nagasaki Medical School) became the medical schools of each university district.
大学南校、開成所、広運館がそれぞれ中学となり、それぞれ第一大学区、第四大学区、第六大学区に属され、大学東校、大阪医学校、長崎医学校が各大学区の医学校となった。