Mibu Roshigumi did not operate smoothly after its establishment, and on May 12, one of the initial members of Mibu Roshigumi, Yoshio TONOUCHI, died there (there has been an assassination theory).
結成当初の壬生浪士組は運営がスムーズに行かず、3月25日に壬生浪士組結成メンバーの一人である殿内義雄は客死した（暗殺説あり）。