During the same year Chokei also died of an illness, which led to the weakening of the Miyoshi clan, and in 1565 Hisahide murdered Yoshiteru ASHIKAGA, the Shogun of the period, in an attempt to gain the hegemony in Kinai.
同年、長慶も病死して三好氏が弱体化すると久秀は畿内における覇権を握ろうと1565年、時の将軍・足利義輝を殺害する。