Around this time in Kyoto in 1565, Miyoshi sanninshu (three chief retainers of the Miyoshi clan, Nagayuki MIYOSHI, Masayasu MIYOSHI and Tomomichi IWANARI), influential people in the Miyoshi clan who had power in the Kinai region (the five capital provinces surrounding the ancient capitals of Nara and Kyoto), and Hisahide MATSUNAGA all collaborated in the murder of the thirteenth seii taishogun, Yoshiteru ASHIKAGA, who had been in increasing conflict with the Miyoshi clan due to his goal of restoring power to the Muromachi bakufu, and installed his cousin Yoshihide ASHIKAGA as the fourteenth shogun to serve as their puppet (Eiroku Incident).
このころ中央では、永禄8年（1565年）、かねて京都を中心に畿内で権勢を誇っていた三好氏の有力者・三好三人衆（三好長逸、三好政康、岩成友通）と松永久秀が、室町幕府権力の復活を目指して三好氏と対立を深めていた第13代征夷大将軍・足利義輝を暗殺し、第14代将軍として義輝の従弟・足利義栄を傀儡として擁立する（永禄の変）。