When YOSHIDA was executed as a result of the Ansei Purge (安政の大獄), the Choshu clan started to clearly indicate its attitude of opposition to Bakufu at all times, which resulted in the Kinmon Rebellion (禁門の変), and twice they were punitively expedited by Bakufu.
安政の大獄によって吉田が処刑されると、これを機に長州藩は終始幕府への敵対心をむき出しにし、その結果禁門の変を起こし、二度に渡る幕府からの征討を受けた。