The opponents, appointing Toshitsura DOI as their leader, began to conspire revocation of Agechi-rei and dismissal of Tadakuni MIZUNO from Roju, and even Tadakuni's most trusted retainers (machi-bugyo (town magistrate) Yozo TORII and kanjo bugyo (commissioner of finance) Tadamoto SAKAKIBARA) revolted to DOI's side; TORII was so resolute that he passed all of Tadakuni's confidential materials to DOI.
反対派は土井利位を盟主に担いで上知令撤回と、水野忠邦の老中免職に動き出し、水野忠邦の主だった腹心達（町奉行鳥居耀蔵、勘定奉行榊原忠職）らも土井派に寝返り、鳥居に至っては忠邦の機密資料を残らず土井に流すという徹底ぶりであった。