Touchdown PCR is another
modification of conventional PCR that may result in a reduction of nonspecific
amplification. It involves the use of an annealing temperature that is higher
than the target optimum in early PCR cycles. The annealing temperature is
decreased by 1°C every cycle or every second cycle until a specified or
'touchdown' annealing temperature is reached. The touchdown temperature is then
used for the remaining number of cycles. This allows for the enrichment of the
correct product over any non-specific product.
Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on
PCR to circumvent spurious priming during gene amplification
File Format: PDF/ Adobe Acrobat
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS.
Nucleic Acids Res. 1991 Jul 25;19(14):4008.
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