Perhaps not amongst die-hard Trump supporters, especially if they're men. But he already seems to have lost a lot of Republican women: 

"This month, about half (47 percent) of Republican female primary voters said they could not imagine themselves voting for Trump. (About 40 percent of male GOP primary voters said the same.)" Compared with 32% of women in that NBC/Wall Street Journal poll saying they couldn't imagine voting for Cruz.  That's a 15-point difference between Republican women on the two candidates.

"Examining Trump's Problem With Female Voters" [Mar 24, 2016]
http://www.nbcnews.com/politics/2016-election/examining-trump-s-problem-female-voters-n544901

And then bring in the *entire* electorate (not just Republicans), & Trump is...well. I'll just say the article is very cheerful news for supporters of Democratic candidates. For the GOP... not so much.