{"action":"created","issue":{"url":"https://api.github.com/repos/nanoporetech/tombo/issues/16","repository_url":"https://api.github.com/repos/nanoporetech/tombo","labels_url":"https://api.github.com/repos/nanoporetech/tombo/issues/16/labels{/name}","comments_url":"https://api.github.com/repos/nanoporetech/tombo/issues/16/comments","events_url":"https://api.github.com/repos/nanoporetech/tombo/issues/16/events","html_url":"https://github.com/nanoporetech/tombo/issues/16","id":284372039,"number":16,"title":"RNA re-squiggle not correct","user":{"login":"haotianteng","id":11155295,"avatar_url":"https://avatars3.githubusercontent.com/u/11155295?v=4","gravatar_id":"","url":"https://api.github.com/users/haotianteng","html_url":"https://github.com/haotianteng","followers_url":"https://api.github.com/users/haotianteng/followers","following_url":"https://api.github.com/users/haotianteng/following{/other_user}","gists_url":"https://api.github.com/users/haotianteng/gists{/gist_id}","starred_url":"https://api.github.com/users/haotianteng/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/haotianteng/subscriptions","organizations_url":"https://api.github.com/users/haotianteng/orgs","repos_url":"https://api.github.com/users/haotianteng/repos","events_url":"https://api.github.com/users/haotianteng/events{/privacy}","received_events_url":"https://api.github.com/users/haotianteng/received_events","type":"User","site_admin":false},"labels":[],"state":"closed","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":4,"created_at":"2017-12-24T16:36:28Z","updated_at":"2018-02-13T05:32:34Z","closed_at":"2017-12-24T17:02:04Z","author_association":"NONE","body":"Hi, I believe that the RNA re-squiggle is not correct.\r\nI test the re-squiggle method on a bacteria dataset(so there is no splicing), and found the re-squiggle gives an abnormal output.\r\nFor example, the first several lines of the re-squiggle output.\r\n\r\n1.7656256796137295\tNaN\t0\t2216\tG\r\n1.753614457831325\tNaN\t2216\t20\tG\r\n1.6867469879518073\tNaN\t2236\t20\tT\r\n1.866265060240964\tNaN\t2256\t20\tG\r\n1.906567167013204\tNaN\t2276\t1269\tG\r\n-1.322520852641335\tNaN\t3545\t26\tT\r\n-1.8988682000730213\tNaN\t3571\t561\tC\r\n\r\nCheck out the attached file.\r\n[pass.tar.gz](https://github.com/nanoporetech/tombo/files/1584948/pass.tar.gz)\r\n\r\nIt seems that tombo didn't reverse the signal when dealing RNA sequencing data."},"comment":{"url":"https://api.github.com/repos/nanoporetech/tombo/issues/comments/365155959","html_url":"https://github.com/nanoporetech/tombo/issues/16#issuecomment-365155959","issue_url":"https://api.github.com/repos/nanoporetech/tombo/issues/16","id":365155959,"user":{"login":"haotianteng","id":11155295,"avatar_url":"https://avatars3.githubusercontent.com/u/11155295?v=4","gravatar_id":"","url":"https://api.github.com/users/haotianteng","html_url":"https://github.com/haotianteng","followers_url":"https://api.github.com/users/haotianteng/followers","following_url":"https://api.github.com/users/haotianteng/following{/other_user}","gists_url":"https://api.github.com/users/haotianteng/gists{/gist_id}","starred_url":"https://api.github.com/users/haotianteng/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/haotianteng/subscriptions","organizations_url":"https://api.github.com/users/haotianteng/orgs","repos_url":"https://api.github.com/users/haotianteng/repos","events_url":"https://api.github.com/users/haotianteng/events{/privacy}","received_events_url":"https://api.github.com/users/haotianteng/received_events","type":"User","site_admin":false},"created_at":"2018-02-13T05:32:34Z","updated_at":"2018-02-13T05:32:34Z","author_association":"NONE","body":"Yes, I am just testing it now using event resquiggle on Albacore1 reads.\nWhat subcommand you recommend to use? event-resquiggle or just resquiggle?\n\n\n\nMarcus Stoiber <notifications@github.com>于2018年2月13日 周二15:28写道：\n\n> This major version update should show much better re-squiggle performance\n> for RNA. If you have a chance to test it out do let me know if the results\n> look better on your data.\n>\n> —\n> You are receiving this because you authored the thread.\n> Reply to this email directly, view it on GitHub\n> <https://github.com/nanoporetech/tombo/issues/16#issuecomment-365155424>,\n> or mute the thread\n> <https://github.com/notifications/unsubscribe-auth/AKo3X5ILTpDkS2WsWxo9z8PHW8Wkf20fks5tUR2TgaJpZM4RL96J>\n> .\n>\n-- \n*Teng Haotian*\nUniversity of Queensland, Queensland, Australia\n+61 0426116017\n"}}
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