{"action":"opened","issue":{"url":"https://api.github.com/repos/benjjneb/dada2/issues/590","repository_url":"https://api.github.com/repos/benjjneb/dada2","labels_url":"https://api.github.com/repos/benjjneb/dada2/issues/590/labels{/name}","comments_url":"https://api.github.com/repos/benjjneb/dada2/issues/590/comments","events_url":"https://api.github.com/repos/benjjneb/dada2/issues/590/events","html_url":"https://github.com/benjjneb/dada2/issues/590","id":371377079,"node_id":"MDU6SXNzdWUzNzEzNzcwNzk=","number":590,"title":"mergePairs failed for example data","user":{"login":"DaliangNing","id":12534157,"node_id":"MDQ6VXNlcjEyNTM0MTU3","avatar_url":"https://avatars2.githubusercontent.com/u/12534157?v=4","gravatar_id":"","url":"https://api.github.com/users/DaliangNing","html_url":"https://github.com/DaliangNing","followers_url":"https://api.github.com/users/DaliangNing/followers","following_url":"https://api.github.com/users/DaliangNing/following{/other_user}","gists_url":"https://api.github.com/users/DaliangNing/gists{/gist_id}","starred_url":"https://api.github.com/users/DaliangNing/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/DaliangNing/subscriptions","organizations_url":"https://api.github.com/users/DaliangNing/orgs","repos_url":"https://api.github.com/users/DaliangNing/repos","events_url":"https://api.github.com/users/DaliangNing/events{/privacy}","received_events_url":"https://api.github.com/users/DaliangNing/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2018-10-18T06:19:26Z","updated_at":"2018-10-18T06:19:26Z","closed_at":null,"author_association":"NONE","body":"mergers <- mergePairs(dadaFs, derepFs, dadaRs, derepRs, verbose=TRUE)\r\n0 paired-reads (in 0 unique pairings) successfully merged out of 7605 (in 421 pairings) input.\r\n0 paired-reads (in 0 unique pairings) successfully merged out of 5692 (in 379 pairings) input.\r\n\r\nFirst time use DADA2. Installed v1.8.0 from bioconductor.\r\nsource(\"https://bioconductor.org/biocLite.R\")\r\nbiocLite(\"dada2\")\r\n\r\ndownloaded example data via the \"example data\" link on tutorial page. use four files of two samples for test.\r\nF3D0_S188_L001_R1_001.fastq\r\nF3D0_S188_L001_R2_001.fastq\r\nF3D1_S189_L001_R1_001.fastq\r\nF3D1_S189_L001_R2_001.fastq\r\n\r\nuse exact R scripts on the tutorial page (set all multithread=FALSE, since using windows).\r\nOther steps are all good, but mergePaires returned \"0 paired-reads\".\r\nOn another computer (Windows), install DADA2 v1.6.0, got the same error.\r\nAny idea or solution for this problem?\r\n"}}
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