{"action":"created","comment":{"url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/comments/207964830","pull_request_review_id":143682024,"id":207964830,"node_id":"MDI0OlB1bGxSZXF1ZXN0UmV2aWV3Q29tbWVudDIwNzk2NDgzMA==","diff_hunk":"@@ -55,3 +55,5 @@ Preparation of cell-sized water-in-oil droplets for *in vitro* reconstitution of\n \n This is a [protocol](https://www.nature.com/protocolexchange/protocols/3815#/related-articles) for the encapsulation of the TX-TL cell-free protein expression system in the liposomes based on the paper by Miyazaki, M, Chiba, M, Ishiwata, S. Preparation of cell-sized water-in-oil droplets for *in vitro* reconstitution of biological processes in cellular compartments. (2015) Nature. DOI: 10.1038/protex.2015.029\n The modified version can be found at [OpenWetWare](https://openwetware.org/wiki/Preparation_of_cell-sized_water-in-oil_droplets) The protocol was used by Malin Jonsson, Miroslav Gasparek (summer 2017 interns), and Zoila Jurado (PhD student at Caltech) to express the GFP in the liposomes in the summer of 2017 in the Murray Lab at Caltech.\n+\n+Kazhito Tabata","path":"protocol-list.md","position":5,"original_position":5,"commit_id":"4b54688f7080ed078bdbf8368ac485cf42f4cf75","original_commit_id":"4b54688f7080ed078bdbf8368ac485cf42f4cf75","user":{"login":"OneScientista","id":42150627,"node_id":"MDQ6VXNlcjQyMTUwNjI3","avatar_url":"https://avatars2.githubusercontent.com/u/42150627?v=4","gravatar_id":"","url":"https://api.github.com/users/OneScientista","html_url":"https://github.com/OneScientista","followers_url":"https://api.github.com/users/OneScientista/followers","following_url":"https://api.github.com/users/OneScientista/following{/other_user}","gists_url":"https://api.github.com/users/OneScientista/gists{/gist_id}","starred_url":"https://api.github.com/users/OneScientista/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/OneScientista/subscriptions","organizations_url":"https://api.github.com/users/OneScientista/orgs","repos_url":"https://api.github.com/users/OneScientista/repos","events_url":"https://api.github.com/users/OneScientista/events{/privacy}","received_events_url":"https://api.github.com/users/OneScientista/received_events","type":"User","site_admin":false},"body":"Add your affiliation","created_at":"2018-08-06T17:09:45Z","updated_at":"2018-08-06T17:09:45Z","html_url":"https://github.com/BuildACell/liposome-kit/pull/15#discussion_r207964830","pull_request_url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15","author_association":"CONTRIBUTOR","_links":{"self":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/comments/207964830"},"html":{"href":"https://github.com/BuildACell/liposome-kit/pull/15#discussion_r207964830"},"pull_request":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15"}}},"pull_request":{"url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15","id":206461419,"node_id":"MDExOlB1bGxSZXF1ZXN0MjA2NDYxNDE5","html_url":"https://github.com/BuildACell/liposome-kit/pull/15","diff_url":"https://github.com/BuildACell/liposome-kit/pull/15.diff","patch_url":"https://github.com/BuildACell/liposome-kit/pull/15.patch","issue_url":"https://api.github.com/repos/BuildACell/liposome-kit/issues/15","number":15,"state":"open","locked":false,"title":"Update protocol-list.md","user":{"login":"Kazuhitotabata","id":42150685,"node_id":"MDQ6VXNlcjQyMTUwNjg1","avatar_url":"https://avatars0.githubusercontent.com/u/42150685?v=4","gravatar_id":"","url":"https://api.github.com/users/Kazuhitotabata","html_url":"https://github.com/Kazuhitotabata","followers_url":"https://api.github.com/users/Kazuhitotabata/followers","following_url":"https://api.github.com/users/Kazuhitotabata/following{/other_user}","gists_url":"https://api.github.com/users/Kazuhitotabata/gists{/gist_id}","starred_url":"https://api.github.com/users/Kazuhitotabata/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/Kazuhitotabata/subscriptions","organizations_url":"https://api.github.com/users/Kazuhitotabata/orgs","repos_url":"https://api.github.com/users/Kazuhitotabata/repos","events_url":"https://api.github.com/users/Kazuhitotabata/events{/privacy}","received_events_url":"https://api.github.com/users/Kazuhitotabata/received_events","type":"User","site_admin":false},"body":"","created_at":"2018-08-06T17:06:59Z","updated_at":"2018-08-06T17:09:45Z","closed_at":null,"merged_at":null,"merge_commit_sha":"fe5301cf60ca4432d73e49983c9046d6c995a9e6","assignee":null,"assignees":[],"requested_reviewers":[],"requested_teams":[],"labels":[],"milestone":null,"commits_url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15/commits","review_comments_url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15/comments","review_comment_url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/comments{/number}","comments_url":"https://api.github.com/repos/BuildACell/liposome-kit/issues/15/comments","statuses_url":"https://api.github.com/repos/BuildACell/liposome-kit/statuses/4b54688f7080ed078bdbf8368ac485cf42f4cf75","head":{"label":"Kazuhitotabata:master","ref":"master","sha":"4b54688f7080ed078bdbf8368ac485cf42f4cf75","user":{"login":"Kazuhitotabata","id":42150685,"node_id":"MDQ6VXNlcjQyMTUwNjg1","avatar_url":"https://avatars0.githubusercontent.com/u/42150685?v=4","gravatar_id":"","url":"https://api.github.com/users/Kazuhitotabata","html_url":"https://github.com/Kazuhitotabata","followers_url":"https://api.github.com/users/Kazuhitotabata/followers","following_url":"https://api.github.com/users/Kazuhitotabata/following{/other_user}","gists_url":"https://api.github.com/users/Kazuhitotabata/gists{/gist_id}","starred_url":"https://api.github.com/users/Kazuhitotabata/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/Kazuhitotabata/subscriptions","organizations_url":"https://api.github.com/users/Kazuhitotabata/orgs","repos_url":"https://api.github.com/users/Kazuhitotabata/repos","events_url":"https://api.github.com/users/Kazuhitotabata/events{/privacy}","received_events_url":"https://api.github.com/users/Kazuhitotabata/received_events","type":"User","site_admin":false},"repo":{"id":143758196,"node_id":"MDEwOlJlcG9zaXRvcnkxNDM3NTgxOTY=","name":"liposome-kit","full_name":"Kazuhitotabata/liposome-kit","owner":{"login":"Kazuhitotabata","id":42150685,"node_id":"MDQ6VXNlcjQyMTUwNjg1","avatar_url":"https://avatars0.githubusercontent.com/u/42150685?v=4","gravatar_id":"","url":"https://api.github.com/users/Kazuhitotabata","html_url":"https://github.com/Kazuhitotabata","followers_url":"https://api.github.com/users/Kazuhitotabata/followers","following_url":"https://api.github.com/users/Kazuhitotabata/following{/other_user}","gists_url":"https://api.github.com/users/Kazuhitotabata/gists{/gist_id}","starred_url":"https://api.github.com/users/Kazuhitotabata/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/Kazuhitotabata/subscriptions","organizations_url":"https://api.github.com/users/Kazuhitotabata/orgs","repos_url":"https://api.github.com/users/Kazuhitotabata/repos","events_url":"https://api.github.com/users/Kazuhitotabata/events{/privacy}","received_events_url":"https://api.github.com/users/Kazuhitotabata/received_events","type":"User","site_admin":false},"private":false,"html_url":"https://github.com/Kazuhitotabata/liposome-kit","description":"Build-A-Cell protocol for making liposomes","fork":true,"url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit","forks_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/forks","keys_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/keys{/key_id}","collaborators_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/collaborators{/collaborator}","teams_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/teams","hooks_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/hooks","issue_events_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/issues/events{/number}","events_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/events","assignees_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/assignees{/user}","branches_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/branches{/branch}","tags_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/tags","blobs_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/git/blobs{/sha}","git_tags_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/git/tags{/sha}","git_refs_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/git/refs{/sha}","trees_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/git/trees{/sha}","statuses_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/statuses/{sha}","languages_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/languages","stargazers_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/stargazers","contributors_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/contributors","subscribers_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/subscribers","subscription_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/subscription","commits_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/commits{/sha}","git_commits_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/git/commits{/sha}","comments_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/comments{/number}","issue_comment_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/issues/comments{/number}","contents_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/contents/{+path}","compare_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/compare/{base}...{head}","merges_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/merges","archive_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/{archive_format}{/ref}","downloads_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/downloads","issues_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/issues{/number}","pulls_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/pulls{/number}","milestones_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/milestones{/number}","notifications_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/notifications{?since,all,participating}","labels_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/labels{/name}","releases_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/releases{/id}","deployments_url":"https://api.github.com/repos/Kazuhitotabata/liposome-kit/deployments","created_at":"2018-08-06T17:00:56Z","updated_at":"2018-08-06T17:06:04Z","pushed_at":"2018-08-06T17:06:02Z","git_url":"git://github.com/Kazuhitotabata/liposome-kit.git","ssh_url":"git@github.com:Kazuhitotabata/liposome-kit.git","clone_url":"https://github.com/Kazuhitotabata/liposome-kit.git","svn_url":"https://github.com/Kazuhitotabata/liposome-kit","homepage":null,"size":91,"stargazers_count":0,"watchers_count":0,"language":"Jupyter Notebook","has_issues":false,"has_projects":true,"has_downloads":true,"has_wiki":true,"has_pages":false,"forks_count":0,"mirror_url":null,"archived":false,"open_issues_count":0,"license":null,"forks":0,"open_issues":0,"watchers":0,"default_branch":"master"}},"base":{"label":"BuildACell:master","ref":"master","sha":"0352e8a1aad2191b4deacfcc86428ecb3aa6a49a","user":{"login":"BuildACell","id":23123180,"node_id":"MDEyOk9yZ2FuaXphdGlvbjIzMTIzMTgw","avatar_url":"https://avatars0.githubusercontent.com/u/23123180?v=4","gravatar_id":"","url":"https://api.github.com/users/BuildACell","html_url":"https://github.com/BuildACell","followers_url":"https://api.github.com/users/BuildACell/followers","following_url":"https://api.github.com/users/BuildACell/following{/other_user}","gists_url":"https://api.github.com/users/BuildACell/gists{/gist_id}","starred_url":"https://api.github.com/users/BuildACell/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/BuildACell/subscriptions","organizations_url":"https://api.github.com/users/BuildACell/orgs","repos_url":"https://api.github.com/users/BuildACell/repos","events_url":"https://api.github.com/users/BuildACell/events{/privacy}","received_events_url":"https://api.github.com/users/BuildACell/received_events","type":"Organization","site_admin":false},"repo":{"id":141645381,"node_id":"MDEwOlJlcG9zaXRvcnkxNDE2NDUzODE=","name":"liposome-kit","full_name":"BuildACell/liposome-kit","owner":{"login":"BuildACell","id":23123180,"node_id":"MDEyOk9yZ2FuaXphdGlvbjIzMTIzMTgw","avatar_url":"https://avatars0.githubusercontent.com/u/23123180?v=4","gravatar_id":"","url":"https://api.github.com/users/BuildACell","html_url":"https://github.com/BuildACell","followers_url":"https://api.github.com/users/BuildACell/followers","following_url":"https://api.github.com/users/BuildACell/following{/other_user}","gists_url":"https://api.github.com/users/BuildACell/gists{/gist_id}","starred_url":"https://api.github.com/users/BuildACell/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/BuildACell/subscriptions","organizations_url":"https://api.github.com/users/BuildACell/orgs","repos_url":"https://api.github.com/users/BuildACell/repos","events_url":"https://api.github.com/users/BuildACell/events{/privacy}","received_events_url":"https://api.github.com/users/BuildACell/received_events","type":"Organization","site_admin":false},"private":false,"html_url":"https://github.com/BuildACell/liposome-kit","description":"Build-A-Cell protocol for making liposomes","fork":false,"url":"https://api.github.com/repos/BuildACell/liposome-kit","forks_url":"https://api.github.com/repos/BuildACell/liposome-kit/forks","keys_url":"https://api.github.com/repos/BuildACell/liposome-kit/keys{/key_id}","collaborators_url":"https://api.github.com/repos/BuildACell/liposome-kit/collaborators{/collaborator}","teams_url":"https://api.github.com/repos/BuildACell/liposome-kit/teams","hooks_url":"https://api.github.com/repos/BuildACell/liposome-kit/hooks","issue_events_url":"https://api.github.com/repos/BuildACell/liposome-kit/issues/events{/number}","events_url":"https://api.github.com/repos/BuildACell/liposome-kit/events","assignees_url":"https://api.github.com/repos/BuildACell/liposome-kit/assignees{/user}","branches_url":"https://api.github.com/repos/BuildACell/liposome-kit/branches{/branch}","tags_url":"https://api.github.com/repos/BuildACell/liposome-kit/tags","blobs_url":"https://api.github.com/repos/BuildACell/liposome-kit/git/blobs{/sha}","git_tags_url":"https://api.github.com/repos/BuildACell/liposome-kit/git/tags{/sha}","git_refs_url":"https://api.github.com/repos/BuildACell/liposome-kit/git/refs{/sha}","trees_url":"https://api.github.com/repos/BuildACell/liposome-kit/git/trees{/sha}","statuses_url":"https://api.github.com/repos/BuildACell/liposome-kit/statuses/{sha}","languages_url":"https://api.github.com/repos/BuildACell/liposome-kit/languages","stargazers_url":"https://api.github.com/repos/BuildACell/liposome-kit/stargazers","contributors_url":"https://api.github.com/repos/BuildACell/liposome-kit/contributors","subscribers_url":"https://api.github.com/repos/BuildACell/liposome-kit/subscribers","subscription_url":"https://api.github.com/repos/BuildACell/liposome-kit/subscription","commits_url":"https://api.github.com/repos/BuildACell/liposome-kit/commits{/sha}","git_commits_url":"https://api.github.com/repos/BuildACell/liposome-kit/git/commits{/sha}","comments_url":"https://api.github.com/repos/BuildACell/liposome-kit/comments{/number}","issue_comment_url":"https://api.github.com/repos/BuildACell/liposome-kit/issues/comments{/number}","contents_url":"https://api.github.com/repos/BuildACell/liposome-kit/contents/{+path}","compare_url":"https://api.github.com/repos/BuildACell/liposome-kit/compare/{base}...{head}","merges_url":"https://api.github.com/repos/BuildACell/liposome-kit/merges","archive_url":"https://api.github.com/repos/BuildACell/liposome-kit/{archive_format}{/ref}","downloads_url":"https://api.github.com/repos/BuildACell/liposome-kit/downloads","issues_url":"https://api.github.com/repos/BuildACell/liposome-kit/issues{/number}","pulls_url":"https://api.github.com/repos/BuildACell/liposome-kit/pulls{/number}","milestones_url":"https://api.github.com/repos/BuildACell/liposome-kit/milestones{/number}","notifications_url":"https://api.github.com/repos/BuildACell/liposome-kit/notifications{?since,all,participating}","labels_url":"https://api.github.com/repos/BuildACell/liposome-kit/labels{/name}","releases_url":"https://api.github.com/repos/BuildACell/liposome-kit/releases{/id}","deployments_url":"https://api.github.com/repos/BuildACell/liposome-kit/deployments","created_at":"2018-07-20T00:52:44Z","updated_at":"2018-08-06T16:59:29Z","pushed_at":"2018-08-06T17:07:38Z","git_url":"git://github.com/BuildACell/liposome-kit.git","ssh_url":"git@github.com:BuildACell/liposome-kit.git","clone_url":"https://github.com/BuildACell/liposome-kit.git","svn_url":"https://github.com/BuildACell/liposome-kit","homepage":null,"size":91,"stargazers_count":2,"watchers_count":2,"language":"Jupyter Notebook","has_issues":true,"has_projects":true,"has_downloads":true,"has_wiki":true,"has_pages":false,"forks_count":10,"mirror_url":null,"archived":false,"open_issues_count":11,"license":null,"forks":10,"open_issues":11,"watchers":2,"default_branch":"master"}},"_links":{"self":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15"},"html":{"href":"https://github.com/BuildACell/liposome-kit/pull/15"},"issue":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/issues/15"},"comments":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/issues/15/comments"},"review_comments":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15/comments"},"review_comment":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/comments{/number}"},"commits":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/pulls/15/commits"},"statuses":{"href":"https://api.github.com/repos/BuildACell/liposome-kit/statuses/4b54688f7080ed078bdbf8368ac485cf42f4cf75"}},"author_association":"NONE"}}
{"actor":{"display_login":"OneScientista"}}