{"action":"opened","issue":{"url":"https://api.github.com/repos/jenniferlu717/Bracken/issues/61","repository_url":"https://api.github.com/repos/jenniferlu717/Bracken","labels_url":"https://api.github.com/repos/jenniferlu717/Bracken/issues/61/labels{/name}","comments_url":"https://api.github.com/repos/jenniferlu717/Bracken/issues/61/comments","events_url":"https://api.github.com/repos/jenniferlu717/Bracken/issues/61/events","html_url":"https://github.com/jenniferlu717/Bracken/issues/61","id":385571284,"node_id":"MDU6SXNzdWUzODU1NzEyODQ=","number":61,"title":"Question about Kraken2 and Bracken for Paired-end analysis","user":{"login":"gc26762524","id":10654271,"node_id":"MDQ6VXNlcjEwNjU0Mjcx","avatar_url":"https://avatars0.githubusercontent.com/u/10654271?v=4","gravatar_id":"","url":"https://api.github.com/users/gc26762524","html_url":"https://github.com/gc26762524","followers_url":"https://api.github.com/users/gc26762524/followers","following_url":"https://api.github.com/users/gc26762524/following{/other_user}","gists_url":"https://api.github.com/users/gc26762524/gists{/gist_id}","starred_url":"https://api.github.com/users/gc26762524/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/gc26762524/subscriptions","organizations_url":"https://api.github.com/users/gc26762524/orgs","repos_url":"https://api.github.com/users/gc26762524/repos","events_url":"https://api.github.com/users/gc26762524/events{/privacy}","received_events_url":"https://api.github.com/users/gc26762524/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2018-11-29T05:02:35Z","updated_at":"2018-11-29T05:02:35Z","closed_at":null,"author_association":"NONE","body":"Hi Jennifer,\r\n\r\nI had the paired reads analysis with length 140bp in Kraken2, but I wonder why only 106 bp reads were considered for Kmers classification such as below. Is there any way I could increase the length?\r\n\r\nU ST-E00600:212:HFFN7ALXX:1:1101:17164:1282 0 140|140 0:106 |:| 0:106\r\nU ST-E00600:212:HFFN7ALXX:1:1101:26928:1282 0 140|140 0:106 |:| 0:106\r\nC ST-E00600:212:HFFN7ALXX:1:1101:7193:1360 213115 140|140 28221:3 2:5 28221:2 2:7 119060:7 2:5 0:16 562:5 2:20 0:28 2:8 |:| 194924:1 2:2 194924:5 2:11 213115:6 2:1 21\r\n3115:10 194924:21 28221:2 194924:2 2:2 28221:3 2:23 0:17\r\nU ST-E00600:212:HFFN7ALXX:1:1101:18747:1360 0 140|140 0:106 |:| 0:106\r\nU ST-E00600:212:HFFN7ALXX:1:1101:20735:1360 0 140|140 0:106 |:| 0:106\r\nC ST-E00600:212:HFFN7ALXX:1:1101:18041:1548 171549 140|140 171549:106 |:| 171549:106\r\n\r\nAlso, I use Bracken for abundance estimation. Should I use 106-mers or 140-mers for Kmer distribution analysis?\r\n\r\nThanks a lot!\r\nCheng"}}
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