{"action":"created","issue":{"url":"https://api.github.com/repos/pombase/curation/issues/1210","repository_url":"https://api.github.com/repos/pombase/curation","labels_url":"https://api.github.com/repos/pombase/curation/issues/1210/labels{/name}","comments_url":"https://api.github.com/repos/pombase/curation/issues/1210/comments","events_url":"https://api.github.com/repos/pombase/curation/issues/1210/events","html_url":"https://github.com/pombase/curation/issues/1210","id":194150380,"node_id":"MDU6SXNzdWUxOTQxNTAzODA=","number":1210,"title":"prp4 annotation check","user":{"login":"ValWood","id":7359272,"node_id":"MDQ6VXNlcjczNTkyNzI=","avatar_url":"https://avatars2.githubusercontent.com/u/7359272?v=4","gravatar_id":"","url":"https://api.github.com/users/ValWood","html_url":"https://github.com/ValWood","followers_url":"https://api.github.com/users/ValWood/followers","following_url":"https://api.github.com/users/ValWood/following{/other_user}","gists_url":"https://api.github.com/users/ValWood/gists{/gist_id}","starred_url":"https://api.github.com/users/ValWood/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ValWood/subscriptions","organizations_url":"https://api.github.com/users/ValWood/orgs","repos_url":"https://api.github.com/users/ValWood/repos","events_url":"https://api.github.com/users/ValWood/events{/privacy}","received_events_url":"https://api.github.com/users/ValWood/received_events","type":"User","site_admin":false},"labels":[{"id":241283122,"node_id":"MDU6TGFiZWwyNDEyODMxMjI=","url":"https://api.github.com/repos/pombase/curation/labels/Ask%20Jacky","name":"Ask Jacky","color":"fad8c7","default":false},{"id":241268158,"node_id":"MDU6TGFiZWwyNDEyNjgxNTg=","url":"https://api.github.com/repos/pombase/curation/labels/next%20curator%20meeting","name":"next curator meeting","color":"bfe5bf","default":false},{"id":994600159,"node_id":"MDU6TGFiZWw5OTQ2MDAxNTk=","url":"https://api.github.com/repos/pombase/curation/labels/tmp","name":"tmp","color":"bfdadc","default":false}],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":1,"created_at":"2016-12-07T19:50:13Z","updated_at":"2018-07-19T10:12:57Z","closed_at":null,"author_association":"MEMBER","body":"\r\nThe temperature-sensitive allele prp4ts caused at the restrictive temperature of 36°C a cell-cycle arrest in the G1 and G2 phases. This phenotype was also observed at the permissive temperature of 25°C when the mutant allele prp4-73ts was expressed from a multicopy plasmid [15] Schwelnus W, Richert K, Opitz F, Gross T, Habara Y, et al. (2001) Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor. EMBO Rep 2: 35–41. PMID: 11252721\r\n\r\nI created these (or will) as “delayed transitions” but existing is\r\nabnormal mitotic cell cycle arrest with unreplicated DNA\r\n\r\nI think there is some insider knowledge on how to interpret the pombe FACS (we should ask Jacky about these)\r\n\r\n\r\nHowever, it was shown that overexpression of Srp1 can suppress the splicing phenotype of the mutant allele prp4-73ts [30]\r\nGroß T, Richert K, Mierke C, Lützelberger M, Käufer NF (1998) Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors. Nucleic Acids Res 26: 505–511. PMID: 9421507. \r\n\r\nThis paper probably isn’t through Canto…\r\n"},"comment":{"url":"https://api.github.com/repos/pombase/curation/issues/comments/406226966","html_url":"https://github.com/pombase/curation/issues/1210#issuecomment-406226966","issue_url":"https://api.github.com/repos/pombase/curation/issues/1210","id":406226966,"node_id":"MDEyOklzc3VlQ29tbWVudDQwNjIyNjk2Ng==","user":{"login":"ValWood","id":7359272,"node_id":"MDQ6VXNlcjczNTkyNzI=","avatar_url":"https://avatars2.githubusercontent.com/u/7359272?v=4","gravatar_id":"","url":"https://api.github.com/users/ValWood","html_url":"https://github.com/ValWood","followers_url":"https://api.github.com/users/ValWood/followers","following_url":"https://api.github.com/users/ValWood/following{/other_user}","gists_url":"https://api.github.com/users/ValWood/gists{/gist_id}","starred_url":"https://api.github.com/users/ValWood/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/ValWood/subscriptions","organizations_url":"https://api.github.com/users/ValWood/orgs","repos_url":"https://api.github.com/users/ValWood/repos","events_url":"https://api.github.com/users/ValWood/events{/privacy}","received_events_url":"https://api.github.com/users/ValWood/received_events","type":"User","site_admin":false},"created_at":"2018-07-19T10:12:56Z","updated_at":"2018-07-19T10:12:56Z","author_association":"MEMBER","body":"\r\ncheck exisitng annotations to prp4\r\n\r\n\"abnormal mitotic cell cycle arrest with unreplicated DNA\" (you can only make this annotation if you only see a G1 peak)\r\n\r\n"}}
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