{"action":"created","issue":{"url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561","repository_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List","labels_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561/labels{/name}","comments_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561/comments","events_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561/events","html_url":"https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/561","id":301759950,"number":561,"title":"Experimental identification of target for Series 3","user":{"login":"holeung","id":10951570,"avatar_url":"https://avatars3.githubusercontent.com/u/10951570?v=4","gravatar_id":"","url":"https://api.github.com/users/holeung","html_url":"https://github.com/holeung","followers_url":"https://api.github.com/users/holeung/followers","following_url":"https://api.github.com/users/holeung/following{/other_user}","gists_url":"https://api.github.com/users/holeung/gists{/gist_id}","starred_url":"https://api.github.com/users/holeung/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/holeung/subscriptions","organizations_url":"https://api.github.com/users/holeung/orgs","repos_url":"https://api.github.com/users/holeung/repos","events_url":"https://api.github.com/users/holeung/events{/privacy}","received_events_url":"https://api.github.com/users/holeung/received_events","type":"User","site_admin":false},"labels":[{"id":39186424,"url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/labels/Collaboration%20Request","name":"Collaboration Request","color":"fbca04","default":false},{"id":78262189,"url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/labels/Series%203","name":"Series 3","color":"2EFE64","default":false}],"state":"open","locked":false,"assignee":{"login":"holeung","id":10951570,"avatar_url":"https://avatars3.githubusercontent.com/u/10951570?v=4","gravatar_id":"","url":"https://api.github.com/users/holeung","html_url":"https://github.com/holeung","followers_url":"https://api.github.com/users/holeung/followers","following_url":"https://api.github.com/users/holeung/following{/other_user}","gists_url":"https://api.github.com/users/holeung/gists{/gist_id}","starred_url":"https://api.github.com/users/holeung/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/holeung/subscriptions","organizations_url":"https://api.github.com/users/holeung/orgs","repos_url":"https://api.github.com/users/holeung/repos","events_url":"https://api.github.com/users/holeung/events{/privacy}","received_events_url":"https://api.github.com/users/holeung/received_events","type":"User","site_admin":false},"assignees":[{"login":"holeung","id":10951570,"avatar_url":"https://avatars3.githubusercontent.com/u/10951570?v=4","gravatar_id":"","url":"https://api.github.com/users/holeung","html_url":"https://github.com/holeung","followers_url":"https://api.github.com/users/holeung/followers","following_url":"https://api.github.com/users/holeung/following{/other_user}","gists_url":"https://api.github.com/users/holeung/gists{/gist_id}","starred_url":"https://api.github.com/users/holeung/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/holeung/subscriptions","organizations_url":"https://api.github.com/users/holeung/orgs","repos_url":"https://api.github.com/users/holeung/repos","events_url":"https://api.github.com/users/holeung/events{/privacy}","received_events_url":"https://api.github.com/users/holeung/received_events","type":"User","site_admin":false}],"milestone":null,"comments":4,"created_at":"2018-03-02T13:04:58Z","updated_at":"2018-03-05T07:16:10Z","closed_at":null,"author_association":"MEMBER","body":"I received a small grant to support experimental, biochemical testing of potential targets for Series 3 (#549) through partnerships with the wonderful core labs (NMR, mass spectrometry, X-ray crystallography, protein expression) of the University of Kansas.\r\n\r\nCurrent approaches that we discussed include:\r\n1. Recombinant expression of candidate kinase/kinase-like targets as discussed in #549. We would prefer to start with those with published expression protocols and that can be expressed in E. coli. We would test for inhibitor binding by saturation transfer difference NMR. We would start by testing our top 5 candidates.\r\n\r\n2. Screening of inhibitors against fractions of Plasmodium lysate or enriched lysate, preferably from a non-infectious Plasmodium species or sterilized P. falciparum. We would screen by STD NMR, affinity mass spectrometry, or by something like DARTS ([https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442491/](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442491)). Anyone know of a source of Plasmodium lysate?\r\n\r\n3. Your idea here! Note that target identification is considered difficult and non-routine.\r\n\r\nI will keep this sub-project open and under the umbrella and spirit of OSM."},"comment":{"url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/comments/370329728","html_url":"https://github.com/OpenSourceMalaria/OSM_To_Do_List/issues/561#issuecomment-370329728","issue_url":"https://api.github.com/repos/OpenSourceMalaria/OSM_To_Do_List/issues/561","id":370329728,"user":{"login":"drc007","id":7080516,"avatar_url":"https://avatars2.githubusercontent.com/u/7080516?v=4","gravatar_id":"","url":"https://api.github.com/users/drc007","html_url":"https://github.com/drc007","followers_url":"https://api.github.com/users/drc007/followers","following_url":"https://api.github.com/users/drc007/following{/other_user}","gists_url":"https://api.github.com/users/drc007/gists{/gist_id}","starred_url":"https://api.github.com/users/drc007/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/drc007/subscriptions","organizations_url":"https://api.github.com/users/drc007/orgs","repos_url":"https://api.github.com/users/drc007/repos","events_url":"https://api.github.com/users/drc007/events{/privacy}","received_events_url":"https://api.github.com/users/drc007/received_events","type":"User","site_admin":false},"created_at":"2018-03-05T07:16:10Z","updated_at":"2018-03-05T07:16:10Z","author_association":"NONE","body":"I suspect ICR will have access to most/all published kinase inhibitors, together with a variety of molecules that illustrate the different hinge binding motifs."}}
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