{"action":"created","issue":{"url":"https://api.github.com/repos/velocyto-team/velocyto.py/issues/51","repository_url":"https://api.github.com/repos/velocyto-team/velocyto.py","labels_url":"https://api.github.com/repos/velocyto-team/velocyto.py/issues/51/labels{/name}","comments_url":"https://api.github.com/repos/velocyto-team/velocyto.py/issues/51/comments","events_url":"https://api.github.com/repos/velocyto-team/velocyto.py/issues/51/events","html_url":"https://github.com/velocyto-team/velocyto.py/issues/51","id":302509157,"node_id":"MDU6SXNzdWUzMDI1MDkxNTc=","number":51,"title":"Compatible with C1 (Fluidigm) read data from SRA??","user":{"login":"topherconley","id":5625193,"node_id":"MDQ6VXNlcjU2MjUxOTM=","avatar_url":"https://avatars0.githubusercontent.com/u/5625193?v=4","gravatar_id":"","url":"https://api.github.com/users/topherconley","html_url":"https://github.com/topherconley","followers_url":"https://api.github.com/users/topherconley/followers","following_url":"https://api.github.com/users/topherconley/following{/other_user}","gists_url":"https://api.github.com/users/topherconley/gists{/gist_id}","starred_url":"https://api.github.com/users/topherconley/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/topherconley/subscriptions","organizations_url":"https://api.github.com/users/topherconley/orgs","repos_url":"https://api.github.com/users/topherconley/repos","events_url":"https://api.github.com/users/topherconley/events{/privacy}","received_events_url":"https://api.github.com/users/topherconley/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":8,"created_at":"2018-03-05T23:48:04Z","updated_at":"2018-10-10T18:15:34Z","closed_at":null,"author_association":"NONE","body":"Hi, \r\n\r\nI have extensively read the documentation and still have questions: \r\n\r\n+ Could you confirm alignments of C1 data (Fluidigm) is valid input to  Velocyto's `run` stage? \r\n+ Further, I have no valid cell barcodes available to me from the SRA sample FASTQs (short read archive). I don't  think that will be a problem, even though you warn against this. \r\n+ Please confirm the \"ValidatedIntrons10X\" logic option (default) is still appropriate for handling C1 data, in spite of the fact that it is not a Drop-Seq based technology? \r\n\r\nThanks!"},"comment":{"url":"https://api.github.com/repos/velocyto-team/velocyto.py/issues/comments/428676295","html_url":"https://github.com/velocyto-team/velocyto.py/issues/51#issuecomment-428676295","issue_url":"https://api.github.com/repos/velocyto-team/velocyto.py/issues/51","id":428676295,"node_id":"MDEyOklzc3VlQ29tbWVudDQyODY3NjI5NQ==","user":{"login":"benslack19","id":20425799,"node_id":"MDQ6VXNlcjIwNDI1Nzk5","avatar_url":"https://avatars0.githubusercontent.com/u/20425799?v=4","gravatar_id":"","url":"https://api.github.com/users/benslack19","html_url":"https://github.com/benslack19","followers_url":"https://api.github.com/users/benslack19/followers","following_url":"https://api.github.com/users/benslack19/following{/other_user}","gists_url":"https://api.github.com/users/benslack19/gists{/gist_id}","starred_url":"https://api.github.com/users/benslack19/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/benslack19/subscriptions","organizations_url":"https://api.github.com/users/benslack19/orgs","repos_url":"https://api.github.com/users/benslack19/repos","events_url":"https://api.github.com/users/benslack19/events{/privacy}","received_events_url":"https://api.github.com/users/benslack19/received_events","type":"User","site_admin":false},"created_at":"2018-10-10T18:15:34Z","updated_at":"2018-10-10T18:15:34Z","author_association":"NONE","body":"Hi @gioelelm and @topherconley , I'm just curious if you had any tips on my case. I'd greatly appreciate any assistance. Do you see anything weird about my environment or BAM? Thank you so much.\r\n\r\nBest,\r\nBen\r\n"}}
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