{"action":"created","issue":{"url":"https://api.github.com/repos/kassambara/ggpubr/issues/134","repository_url":"https://api.github.com/repos/kassambara/ggpubr","labels_url":"https://api.github.com/repos/kassambara/ggpubr/issues/134/labels{/name}","comments_url":"https://api.github.com/repos/kassambara/ggpubr/issues/134/comments","events_url":"https://api.github.com/repos/kassambara/ggpubr/issues/134/events","html_url":"https://github.com/kassambara/ggpubr/issues/134","id":379706953,"node_id":"MDU6SXNzdWUzNzk3MDY5NTM=","number":134,"title":"compute p-value by gene (t-test) on qPCR data","user":{"login":"lmanchon","id":3015749,"node_id":"MDQ6VXNlcjMwMTU3NDk=","avatar_url":"https://avatars0.githubusercontent.com/u/3015749?v=4","gravatar_id":"","url":"https://api.github.com/users/lmanchon","html_url":"https://github.com/lmanchon","followers_url":"https://api.github.com/users/lmanchon/followers","following_url":"https://api.github.com/users/lmanchon/following{/other_user}","gists_url":"https://api.github.com/users/lmanchon/gists{/gist_id}","starred_url":"https://api.github.com/users/lmanchon/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/lmanchon/subscriptions","organizations_url":"https://api.github.com/users/lmanchon/orgs","repos_url":"https://api.github.com/users/lmanchon/repos","events_url":"https://api.github.com/users/lmanchon/events{/privacy}","received_events_url":"https://api.github.com/users/lmanchon/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":1,"created_at":"2018-11-12T10:16:07Z","updated_at":"2018-11-14T08:21:28Z","closed_at":null,"author_association":"NONE","body":"--Hi,\r\n\r\ni work on table shaped as follow:\r\n            group  gene normalized  calibrated relative_expression      error        lower        upper   color\r\n1   H9_NT_LSB_D16 ASCL1  10.997000  3.55566692         -3.55566692 0.20989458  0.073528048 9.836083e-02 #42c7ce\r\n2   H9_NT_LSB_D20 ASCL1  10.041000  2.59966692         -2.59966692 0.14456394  0.149246514 1.823645e-01 #42c7ce\r\n3   H9_NT_LSB_D27 ASCL1   7.441333  0.00000000          0.00000000 0.06947961  0.952981682 1.049338e+00 #42c7ce\r\n4  H9_Ri1_LSB_D16 ASCL1  14.511000  7.06966718         -7.06966718 0.21513200  0.006412936 8.641304e-03 #42c7ce\r\n5  H9_Ri1_LSB_D20 ASCL1  13.145333  5.70400048         -5.70400048 0.10920400  0.017784886 2.069181e-02 #42c7ce\r\n6  H9_Ri1_LSB_D27 ASCL1   3.259334 -4.18199920          4.18199920 9.34894129  0.027835270 1.183638e+04 #f67468\r\n7  H9_Ri3_LSB_D16 ASCL1  14.167666  6.72633330         -6.72633330 0.34238223  0.007449114 1.197400e-02 #42c7ce\r\n8  H9_Ri3_LSB_D20 ASCL1  12.073000  4.63166714         -4.63166714 0.03499479  0.039372662 4.132984e-02 #42c7ce\r\n9  H9_Ri3_LSB_D27 ASCL1   8.715000  1.27366702         -1.27366702 0.10553967  0.384430129 4.449986e-01 #42c7ce\r\n10  H9_NT_LSB_D16 FOXG1  15.478000  3.38800017         -3.38800017 0.21835466  0.082106780 1.111326e-01 #42c7ce\r\n11  H9_NT_LSB_D20 FOXG1  11.442667 -0.64733282          0.64733282 0.13629949  1.425070539 1.721460e+00 #f67468\r\n12  H9_NT_LSB_D27 FOXG1  12.090000  0.00000000          0.00000000 0.12898911  0.914471990 1.093527e+00 #42c7ce\r\n13 H9_Ri1_LSB_D16 FOXG1  14.312666  2.22266643         -2.22266643 0.31353510  0.172396032 2.662528e-01 #42c7ce\r\n14 H9_Ri1_LSB_D20 FOXG1  12.441000  0.35100047         -0.35100047 0.11337113  0.724786708 8.481378e-01 #42c7ce\r\n15 H9_Ri1_LSB_D27 FOXG1   9.196000 -2.89399973          2.89399973 9.35114100  0.011381691 4.854614e+03 #f67468\r\n16 H9_Ri3_LSB_D16 FOXG1  16.024333  3.93433349         -3.93433349 0.56043311  0.044354780 9.646167e-02 #42c7ce\r\n17 H9_Ri3_LSB_D20 FOXG1  12.318333  0.22833284         -0.22833284 0.23950700  0.723046414 1.007775e+00 #42c7ce\r\n18 H9_Ri3_LSB_D27 FOXG1  11.347667 -0.74233309          0.74233309 0.04958543  1.616358996 1.731375e+00 #f67468\r\n19  H9_NT_LSB_D16 HOXB4  16.134666  1.04566605         -1.04566605 0.30914201  0.390986834 6.001837e-01 #42c7ce\r\n20  H9_NT_LSB_D20 HOXB4  15.334667  0.24566618         -0.24566618 0.21284675  0.727735991 9.775081e-01 #42c7ce\r\n21  H9_NT_LSB_D27 HOXB4  15.089000  0.00000000          0.00000000 0.07253994  0.950962304 1.051566e+00 #42c7ce\r\n22 H9_Ri1_LSB_D16 HOXB4  15.789667  0.70066643         -0.70066643 0.23817304  0.521652340 7.257309e-01 #42c7ce\r\n23 H9_Ri1_LSB_D20 HOXB4  15.530667  0.44166628         -0.44166628 0.12390913  0.675685870 8.023162e-01 #42c7ce\r\n24 H9_Ri1_LSB_D27 HOXB4  10.275333 -4.81366730          4.81366730 9.34877326  0.043131749 1.833663e+04 #f67468\r\n25 H9_Ri3_LSB_D16 HOXB4  16.060333  0.97133223         -0.97133223 0.41698356  0.382010503 6.809644e-01 #42c7ce\r\n26 H9_Ri3_LSB_D20 HOXB4  15.033000 -0.05600008          0.05600008 0.13453065  0.947021725 1.141183e+00 #f67468\r\n27 H9_Ri3_LSB_D27 HOXB4  15.536000  0.44699923         -0.44699923 0.30776057  0.592645051 9.079982e-01 #42c7ce\r\n28  H9_NT_LSB_D16  PAX6   5.184999 -0.45766671          0.45766671 0.19210101  1.202107324 1.568916e+00 #f67468\r\n29  H9_NT_LSB_D20  PAX6   4.704000 -0.93866634          0.93866634 0.09125535  1.799269121 2.041913e+00 #f67468\r\n30  H9_NT_LSB_D27  PAX6   5.642666  0.00000000          0.00000000 0.07359626  0.950266280 1.052337e+00 #42c7ce\r\n31 H9_Ri1_LSB_D16  PAX6   2.239000 -3.40366618          3.40366618 0.24029512  8.959207100 1.250091e+01 #f67468\r\n32 H9_Ri1_LSB_D20  PAX6   3.418000 -2.22466659          2.22466659 0.03218695  4.570904404 4.779480e+00 #f67468\r\n33 H9_Ri1_LSB_D27  PAX6  -2.332000 -7.97466659          7.97466659 9.34875971  0.385795143 1.640102e+05 #f67468\r\n34 H9_Ri3_LSB_D16  PAX6   2.638333 -3.00433318          3.00433318 0.28261153  6.596595505 9.760430e+00 #f67468\r\n35 H9_Ri3_LSB_D20  PAX6   4.272999 -1.36966674          1.36966674 0.05022576  2.495693865 2.675656e+00 #f67468\r\n36 H9_Ri3_LSB_D27  PAX6   2.903334 -2.73933220          2.73933220 0.03814863  6.503352260 6.856540e+00 #f67468\r\n37  H9_NT_LSB_D16   RFX  14.000000  3.42900054         -3.42900054 0.45305080  0.067824422 1.271013e-01 #42c7ce\r\n38  H9_NT_LSB_D20   RFX   9.471334 -1.09966564          1.09966564 0.08118040  2.025790859 2.267097e+00 #f67468\r\n39  H9_NT_LSB_D27   RFX  10.570999  0.00000000          0.00000000 0.07146385  0.951671882 1.050782e+00 #42c7ce\r\n40 H9_Ri1_LSB_D16   RFX  12.638333  2.06733386         -2.06733386 0.44233702  0.175595664 3.242106e-01 #42c7ce\r\n41 H9_Ri1_LSB_D20   RFX  12.339667  1.76866722         -1.76866722 0.22879153  0.250440752 3.439151e-01 #42c7ce\r\n42 H9_Ri1_LSB_D27   RFX   5.362333 -5.20866648          5.20866648 9.34876427  0.056716103 2.411145e+04 #f67468\r\n43 H9_Ri3_LSB_D16   RFX  11.253333  0.68233331         -0.68233331 0.27621934  0.514572888 7.546534e-01 #42c7ce\r\n44 H9_Ri3_LSB_D20   RFX  11.055000  0.48400084         -0.48400084 0.20540179  0.620110561 8.243912e-01 #42c7ce\r\n45 H9_Ri3_LSB_D27   RFX   8.489000 -2.08199946          2.08199946 0.03430261  4.134454085 4.335812e+00 #f67468\r\n\r\ni want to compute p-value on each gene based on his qPCR relative-expression, i don't know how to order my data to run compare_means.\r\n\r\nWhen i run this command:\r\n compare_means(gene ~ relative_expression, data = result, ref.group = \"0\", method = \"t.test\")\r\nit returns this error:\r\nError in t.test.default(xi, xj, paired = paired, alternative = alternative,  : \r\n  not enough 'x' observations\r\n\r\nthank you for your help.\r\nL.M.\r\n\r\n\r\n\r\n\r\n"},"comment":{"url":"https://api.github.com/repos/kassambara/ggpubr/issues/comments/438576783","html_url":"https://github.com/kassambara/ggpubr/issues/134#issuecomment-438576783","issue_url":"https://api.github.com/repos/kassambara/ggpubr/issues/134","id":438576783,"node_id":"MDEyOklzc3VlQ29tbWVudDQzODU3Njc4Mw==","user":{"login":"lmanchon","id":3015749,"node_id":"MDQ6VXNlcjMwMTU3NDk=","avatar_url":"https://avatars0.githubusercontent.com/u/3015749?v=4","gravatar_id":"","url":"https://api.github.com/users/lmanchon","html_url":"https://github.com/lmanchon","followers_url":"https://api.github.com/users/lmanchon/followers","following_url":"https://api.github.com/users/lmanchon/following{/other_user}","gists_url":"https://api.github.com/users/lmanchon/gists{/gist_id}","starred_url":"https://api.github.com/users/lmanchon/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/lmanchon/subscriptions","organizations_url":"https://api.github.com/users/lmanchon/orgs","repos_url":"https://api.github.com/users/lmanchon/repos","events_url":"https://api.github.com/users/lmanchon/events{/privacy}","received_events_url":"https://api.github.com/users/lmanchon/received_events","type":"User","site_admin":false},"created_at":"2018-11-14T08:21:28Z","updated_at":"2018-11-14T08:21:28Z","author_association":"NONE","body":"--Sorry,\r\ni upload my file here:\r\n[results.txt](https://github.com/kassambara/ggpubr/files/2579756/results.txt)\r\nthank you--\r\n\r\n"}}
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