{"action":"closed","issue":{"url":"https://api.github.com/repos/mothur/mothur/issues/455","repository_url":"https://api.github.com/repos/mothur/mothur","labels_url":"https://api.github.com/repos/mothur/mothur/issues/455/labels{/name}","comments_url":"https://api.github.com/repos/mothur/mothur/issues/455/comments","events_url":"https://api.github.com/repos/mothur/mothur/issues/455/events","html_url":"https://github.com/mothur/mothur/issues/455","id":324885845,"node_id":"MDU6SXNzdWUzMjQ4ODU4NDU=","number":455,"title":"OTU tree problems","user":{"login":"juanjuanignacio","id":25993758,"node_id":"MDQ6VXNlcjI1OTkzNzU4","avatar_url":"https://avatars3.githubusercontent.com/u/25993758?v=4","gravatar_id":"","url":"https://api.github.com/users/juanjuanignacio","html_url":"https://github.com/juanjuanignacio","followers_url":"https://api.github.com/users/juanjuanignacio/followers","following_url":"https://api.github.com/users/juanjuanignacio/following{/other_user}","gists_url":"https://api.github.com/users/juanjuanignacio/gists{/gist_id}","starred_url":"https://api.github.com/users/juanjuanignacio/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/juanjuanignacio/subscriptions","organizations_url":"https://api.github.com/users/juanjuanignacio/orgs","repos_url":"https://api.github.com/users/juanjuanignacio/repos","events_url":"https://api.github.com/users/juanjuanignacio/events{/privacy}","received_events_url":"https://api.github.com/users/juanjuanignacio/received_events","type":"User","site_admin":false},"labels":[{"id":303524696,"node_id":"MDU6TGFiZWwzMDM1MjQ2OTY=","url":"https://api.github.com/repos/mothur/mothur/labels/Support","name":"Support","color":"bfe5bf","default":false}],"state":"closed","locked":false,"assignee":null,"assignees":[],"milestone":{"url":"https://api.github.com/repos/mothur/mothur/milestones/7","html_url":"https://github.com/mothur/mothur/milestone/7","labels_url":"https://api.github.com/repos/mothur/mothur/milestones/7/labels","id":1473828,"node_id":"MDk6TWlsZXN0b25lMTQ3MzgyOA==","number":7,"title":"Bug / Support Tracking","description":"Goals\r\n\r\n* Help find problem areas in the code through better bug tracking.\r\n* Improve documentation by identifying areas where additional support is requested.\r\n","creator":{"login":"mothur-westcott","id":1379958,"node_id":"MDQ6VXNlcjEzNzk5NTg=","avatar_url":"https://avatars3.githubusercontent.com/u/1379958?v=4","gravatar_id":"","url":"https://api.github.com/users/mothur-westcott","html_url":"https://github.com/mothur-westcott","followers_url":"https://api.github.com/users/mothur-westcott/followers","following_url":"https://api.github.com/users/mothur-westcott/following{/other_user}","gists_url":"https://api.github.com/users/mothur-westcott/gists{/gist_id}","starred_url":"https://api.github.com/users/mothur-westcott/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/mothur-westcott/subscriptions","organizations_url":"https://api.github.com/users/mothur-westcott/orgs","repos_url":"https://api.github.com/users/mothur-westcott/repos","events_url":"https://api.github.com/users/mothur-westcott/events{/privacy}","received_events_url":"https://api.github.com/users/mothur-westcott/received_events","type":"User","site_admin":false},"open_issues":21,"closed_issues":39,"state":"open","created_at":"2015-12-24T17:40:44Z","updated_at":"2018-11-09T15:37:48Z","due_on":null,"closed_at":null},"comments":2,"created_at":"2018-05-21T11:41:20Z","updated_at":"2018-11-09T15:37:48Z","closed_at":"2018-11-09T15:37:48Z","author_association":"NONE","body":"\r\nHello,\r\n I am using mothur for metagenomic analysis and everything is fine, until my last step that is to get an OTU tree. I already have a biom, a consensus of the taxonomy, with the command otu.rep as you told me I got the fasta of the otus (500mb), which is a msa. But, now, im trying to do a tree. I have tried all the ways that have occurred to me. I have tried to make a distance matrix (phylip) of this fasta but the matrix has 200gb since it does not support the cutoff command.\r\nUsing clearcut, the process lasts forever since it seems that my fasta is too big. I need some help or some suggestion on how I could do this. Thank you."}}
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