{"action":"opened","issue":{"url":"https://api.github.com/repos/CGATOxford/UMI-tools/issues/296","repository_url":"https://api.github.com/repos/CGATOxford/UMI-tools","labels_url":"https://api.github.com/repos/CGATOxford/UMI-tools/issues/296/labels{/name}","comments_url":"https://api.github.com/repos/CGATOxford/UMI-tools/issues/296/comments","events_url":"https://api.github.com/repos/CGATOxford/UMI-tools/issues/296/events","html_url":"https://github.com/CGATOxford/UMI-tools/issues/296","id":378409566,"node_id":"MDU6SXNzdWUzNzg0MDk1NjY=","number":296,"title":"umi_tools whitelist barcode and umi only in the read2, but I found that  the barcode sequence not from the first base","user":{"login":"Krascal","id":11272000,"node_id":"MDQ6VXNlcjExMjcyMDAw","avatar_url":"https://avatars2.githubusercontent.com/u/11272000?v=4","gravatar_id":"","url":"https://api.github.com/users/Krascal","html_url":"https://github.com/Krascal","followers_url":"https://api.github.com/users/Krascal/followers","following_url":"https://api.github.com/users/Krascal/following{/other_user}","gists_url":"https://api.github.com/users/Krascal/gists{/gist_id}","starred_url":"https://api.github.com/users/Krascal/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/Krascal/subscriptions","organizations_url":"https://api.github.com/users/Krascal/orgs","repos_url":"https://api.github.com/users/Krascal/repos","events_url":"https://api.github.com/users/Krascal/events{/privacy}","received_events_url":"https://api.github.com/users/Krascal/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2018-11-07T18:16:55Z","updated_at":"2018-11-07T18:16:55Z","closed_at":null,"author_association":"NONE","body":"In the data I analyzed, the paper said the barcode is the first 8 bps in read2, and the next 8 bps is the UMI, so the read1 didn't have the barcode/umi information. \r\nI used the instruction as below:\r\numi_tools whitelist -p=CCCCCCCCNNNNNNNN -L=test.exatract.log --stdin=test2.fastq > test.whitelist\r\nbut I found that the barcodes is not the first 8bps of read2, but is the 2-9th bp in read2, is this correct? And why?"}}
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