{"action":"created","issue":{"url":"https://api.github.com/repos/satijalab/seurat/issues/804","repository_url":"https://api.github.com/repos/satijalab/seurat","labels_url":"https://api.github.com/repos/satijalab/seurat/issues/804/labels{/name}","comments_url":"https://api.github.com/repos/satijalab/seurat/issues/804/comments","events_url":"https://api.github.com/repos/satijalab/seurat/issues/804/events","html_url":"https://github.com/satijalab/seurat/issues/804","id":364711941,"node_id":"MDU6SXNzdWUzNjQ3MTE5NDE=","number":804,"title":"How to pull out OPN4-positive cells","user":{"login":"1988general","id":38145359,"node_id":"MDQ6VXNlcjM4MTQ1MzU5","avatar_url":"https://avatars0.githubusercontent.com/u/38145359?v=4","gravatar_id":"","url":"https://api.github.com/users/1988general","html_url":"https://github.com/1988general","followers_url":"https://api.github.com/users/1988general/followers","following_url":"https://api.github.com/users/1988general/following{/other_user}","gists_url":"https://api.github.com/users/1988general/gists{/gist_id}","starred_url":"https://api.github.com/users/1988general/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/1988general/subscriptions","organizations_url":"https://api.github.com/users/1988general/orgs","repos_url":"https://api.github.com/users/1988general/repos","events_url":"https://api.github.com/users/1988general/events{/privacy}","received_events_url":"https://api.github.com/users/1988general/received_events","type":"User","site_admin":false},"labels":[{"id":213735095,"node_id":"MDU6TGFiZWwyMTM3MzUwOTU=","url":"https://api.github.com/repos/satijalab/seurat/labels/question","name":"question","color":"cc317c","default":true}],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":1,"created_at":"2018-09-28T01:52:24Z","updated_at":"2018-10-02T18:12:34Z","closed_at":null,"author_association":"NONE","body":"Hi,\r\nI want to do a subclassification within OPN4-positive cells. I am using Seurat. I really don’t know how to pull out the OPN4-positive cells. My input file is filtered gene matrix from 10x cellranger count. Could you provide some code for this?\r\n\r\nThanks a lot! \r\nJing\r\n"},"comment":{"url":"https://api.github.com/repos/satijalab/seurat/issues/comments/426376243","html_url":"https://github.com/satijalab/seurat/issues/804#issuecomment-426376243","issue_url":"https://api.github.com/repos/satijalab/seurat/issues/804","id":426376243,"node_id":"MDEyOklzc3VlQ29tbWVudDQyNjM3NjI0Mw==","user":{"login":"CifICare","id":43524354,"node_id":"MDQ6VXNlcjQzNTI0MzU0","avatar_url":"https://avatars0.githubusercontent.com/u/43524354?v=4","gravatar_id":"","url":"https://api.github.com/users/CifICare","html_url":"https://github.com/CifICare","followers_url":"https://api.github.com/users/CifICare/followers","following_url":"https://api.github.com/users/CifICare/following{/other_user}","gists_url":"https://api.github.com/users/CifICare/gists{/gist_id}","starred_url":"https://api.github.com/users/CifICare/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/CifICare/subscriptions","organizations_url":"https://api.github.com/users/CifICare/orgs","repos_url":"https://api.github.com/users/CifICare/repos","events_url":"https://api.github.com/users/CifICare/events{/privacy}","received_events_url":"https://api.github.com/users/CifICare/received_events","type":"User","site_admin":false},"created_at":"2018-10-02T18:12:34Z","updated_at":"2018-10-02T18:12:34Z","author_association":"NONE","body":"You can also use the do.identify function with GenePlot or FeaturePlot to manually select the cells that express OPN4:\r\n\r\nCell.Seleceted <- GenePlot(Object, ident.1=\"OPN4\", do.identify=TRUE)\r\nCell.Seleceted <- FeaturePlot(Object, genes.use=\"OPN4\", do.identify=TRUE)"}}
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