{"action":"opened","issue":{"url":"https://api.github.com/repos/marbl/canu/issues/949","repository_url":"https://api.github.com/repos/marbl/canu","labels_url":"https://api.github.com/repos/marbl/canu/issues/949/labels{/name}","comments_url":"https://api.github.com/repos/marbl/canu/issues/949/comments","events_url":"https://api.github.com/repos/marbl/canu/issues/949/events","html_url":"https://github.com/marbl/canu/issues/949","id":331081534,"node_id":"MDU6SXNzdWUzMzEwODE1MzQ=","number":949,"title":"Failed to open 'ovlStore.BUILDING/0001<001>' for writing: Protocol error","user":{"login":"taeminla","id":40155785,"node_id":"MDQ6VXNlcjQwMTU1Nzg1","avatar_url":"https://avatars1.githubusercontent.com/u/40155785?v=4","gravatar_id":"","url":"https://api.github.com/users/taeminla","html_url":"https://github.com/taeminla","followers_url":"https://api.github.com/users/taeminla/followers","following_url":"https://api.github.com/users/taeminla/following{/other_user}","gists_url":"https://api.github.com/users/taeminla/gists{/gist_id}","starred_url":"https://api.github.com/users/taeminla/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/taeminla/subscriptions","organizations_url":"https://api.github.com/users/taeminla/orgs","repos_url":"https://api.github.com/users/taeminla/repos","events_url":"https://api.github.com/users/taeminla/events{/privacy}","received_events_url":"https://api.github.com/users/taeminla/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2018-06-11T07:57:06Z","updated_at":"2018-06-11T07:57:06Z","closed_at":null,"author_association":"NONE","body":"Hello canu team, \r\n \r\ni'm using canu 1.7v to assemble approximate 2.0m bacterial genome with nanopore reads running on virtual machine.\r\n\r\nHere is my command  \r\n./canu -p lacto -d /media/sf_SF/a/ genomeSize=2.0m gnuplotTested=true  -nanopore-raw /media/sf_SF/lacto/fasta/gz\r\n\r\nafter a few minute, it said 'Overlap store failed, tried 2 times, giving up' \r\n\r\nAnd here is ovlStore.err \r\n\r\n-- SCANNING INPUTS --\r\n\r\n      Molaps\r\n------------ ----------------------------------------\r\n      11.966          1-overlapper/results/000001.ovb\r\n      10.627          1-overlapper/results/000002.ovb\r\n       1.487          1-overlapper/results/000003.ovb\r\n------------ ----------------------------------------\r\n      24.080 overlaps in inputs\r\n      48.160 overlaps to sort\r\n\r\n\r\nAllocating space for 48159706 overlaps.\r\n\r\n\r\n-- LOADING OVERLAPS --\r\n\r\n       Input       Loaded Percent\r\n      Molaps       Molaps  Loaded\r\n------------ ------------ ------- ----------------------------------------\r\n      48.160        0.000   0.00%          1-overlapper/results/000001.ovb\r\n      48.160       23.931   0.00%          1-overlapper/results/000002.ovb\r\n      48.160       30.972   0.00%\r\n      48.160       45.186   0.00%          1-overlapper/results/000003.ovb\r\n------------ ------------ ------- ----------------------------------------\r\n      48.160       48.160   0.00%\r\n\r\n-- OVERLAP FILTERING --\r\n\r\nDEDUPE OVERLAPS\r\nSaved      48159706 dedupe overlaps\r\nDiscarded  0 don't care\r\nDiscarded  0 different library\r\nDiscarded  0 obviously not duplicates\r\n\r\nTRIMMING OVERLAPS\r\nSaved      1390458 trimming overlaps\r\nDiscarded  0 don't care\r\nDiscarded  11868384 too similar\r\nDiscarded  34900864 too short\r\n\r\nUNITIGGING OVERLAPS\r\nSaved      48159706 unitigging overlaps\r\n\r\nDiscarded  0 low quality, more than 1.0000 fraction error\r\nDiscarded  0 opposite orientation\r\n\r\n\r\n-- SORT OVERLAPS --\r\n\r\n\r\n-- OUTPUT OVERLAPS --\r\n\r\nFailed to open './lacto.ovlStore.BUILDING/0001<001>' for writing: Protocol error\r\n\r\ni don't know what problem is. \r\n\r\nThank you \r\nBest Regards \r\nTaemin \r\n\r\n"}}
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